Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074354.t1 | XP_002946472.1 | 98.6 | 73 | 1 | 0 | 1 | 73 | 1 | 73 | 7.40E-34 | 152.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074354.t1 | Q9FGZ9 | 93.2 | 73 | 5 | 0 | 1 | 73 | 1 | 73 | 4.9e-34 | 144.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074354.t1 | D8TJM7 | 98.6 | 73 | 1 | 0 | 1 | 73 | 1 | 73 | 5.4e-34 | 152.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene074354 | MS.gene028709 | PPI |
| MS.gene074354 | MS.gene045369 | PPI |
| MS.gene074354 | MS.gene79884 | PPI |
| MS.gene074354 | MS.gene028711 | PPI |
| MS.gene074354 | MS.gene31284 | PPI |
| MS.gene074354 | MS.gene058936 | PPI |
| MS.gene074354 | MS.gene77522 | PPI |
| MS.gene074354 | MS.gene75680 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074354.t1 | MTR_3g084140 | 95.890 | 73 | 3 | 0 | 1 | 73 | 1 | 73 | 5.55e-47 | 144 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074354.t1 | AT3G45180 | 93.151 | 73 | 5 | 0 | 1 | 73 | 1 | 73 | 4.11e-46 | 141 |
| MS.gene074354.t1 | AT3G45180 | 93.151 | 73 | 5 | 0 | 1 | 73 | 1 | 73 | 4.11e-46 | 141 |
| MS.gene074354.t1 | AT5G42300 | 93.151 | 73 | 5 | 0 | 1 | 73 | 1 | 73 | 1.30e-45 | 140 |
Find 12 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAAAATTCGGATTCAGAAA+TGG | 0.431017 | 2.2:+6838870 | MS.gene074354:CDS |
| GTACAAGACCTGACAAAATT+CGG | 0.445316 | 2.2:+6838858 | MS.gene074354:CDS |
| GAACGATCGGTTAGGGAAGA+AGG | 0.472486 | 2.2:+6838769 | MS.gene074354:CDS |
| AAACTCGTTGCTGCACAAAC+AGG | 0.476026 | 2.2:+6838836 | MS.gene074354:CDS |
| ATTAGTTATAGTAGAGTTCG+AGG | 0.477137 | 2.2:-6838952 | None:intergenic |
| TCGATCGAAGAAGATGATAG+AGG | 0.558716 | 2.2:+6838739 | None:intergenic |
| TACGAGATTCATGATGGCAT+GGG | 0.579214 | 2.2:+6838929 | MS.gene074354:CDS |
| TTACGAGATTCATGATGGCA+TGG | 0.596991 | 2.2:+6838928 | MS.gene074354:CDS |
| TCTGATTACGAGATTCATGA+TGG | 0.607663 | 2.2:+6838923 | MS.gene074354:CDS |
| GAGTTTCTTCAAGTCACCGA+TGG | 0.637090 | 2.2:-6838819 | None:intergenic |
| ATCGAAGAAGATGATAGAGG+TGG | 0.670468 | 2.2:+6838742 | None:intergenic |
| TGCAACGAAGATGATACCAT+CGG | 0.675479 | 2.2:+6838803 | MS.gene074354:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACAAAATTCGGATTCAGAAA+TGG | + | chr2.2:6838870-6838889 | MS.gene074354:CDS | 30.0% | |
| GTACAAGACCTGACAAAATT+CGG | + | chr2.2:6838858-6838877 | MS.gene074354:CDS | 35.0% | |
| TCTGATTACGAGATTCATGA+TGG | + | chr2.2:6838923-6838942 | MS.gene074354:CDS | 35.0% | |
| ! | TTCTGAATCCGAATTTTGTC+AGG | - | chr2.2:6838869-6838888 | None:intergenic | 35.0% |
| TACGAGATTCATGATGGCAT+GGG | + | chr2.2:6838929-6838948 | MS.gene074354:CDS | 40.0% | |
| TGCAACGAAGATGATACCAT+CGG | + | chr2.2:6838803-6838822 | MS.gene074354:CDS | 40.0% | |
| TTACGAGATTCATGATGGCA+TGG | + | chr2.2:6838928-6838947 | MS.gene074354:CDS | 40.0% | |
| !!! | TAGAGGTGGTTTTGAACGAT+CGG | + | chr2.2:6838756-6838775 | MS.gene074354:CDS | 40.0% |
| !!! | TGGTTTTGAACGATCGGTTA+GGG | + | chr2.2:6838762-6838781 | MS.gene074354:CDS | 40.0% |
| AAACTCGTTGCTGCACAAAC+AGG | + | chr2.2:6838836-6838855 | MS.gene074354:CDS | 45.0% | |
| GAGTTTCTTCAAGTCACCGA+TGG | - | chr2.2:6838822-6838841 | None:intergenic | 45.0% | |
| !!! | GTGGTTTTGAACGATCGGTT+AGG | + | chr2.2:6838761-6838780 | MS.gene074354:CDS | 45.0% |
| GAACGATCGGTTAGGGAAGA+AGG | + | chr2.2:6838769-6838788 | MS.gene074354:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.2 | gene | 6838752 | 6838973 | 6838752 | ID=MS.gene074354 |
| chr2.2 | mRNA | 6838752 | 6838973 | 6838752 | ID=MS.gene074354.t1;Parent=MS.gene074354 |
| chr2.2 | exon | 6838752 | 6838973 | 6838752 | ID=MS.gene074354.t1.exon1;Parent=MS.gene074354.t1 |
| chr2.2 | CDS | 6838752 | 6838973 | 6838752 | ID=cds.MS.gene074354.t1;Parent=MS.gene074354.t1 |
| Gene Sequence |
| Protein sequence |
