Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene045369.t1 | XP_003601666.1 | 100 | 73 | 0 | 0 | 1 | 73 | 1 | 73 | 5.70E-34 | 152.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene045369.t1 | Q9FGZ9 | 94.5 | 73 | 4 | 0 | 1 | 73 | 1 | 73 | 3.7e-34 | 144.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene045369.t1 | G7JAQ0 | 100.0 | 73 | 0 | 0 | 1 | 73 | 1 | 73 | 4.1e-34 | 152.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene045369 | MS.gene058936 | PPI |
| MS.gene058936 | MS.gene045369 | PPI |
| MS.gene31284 | MS.gene045369 | PPI |
| MS.gene79884 | MS.gene045369 | PPI |
| MS.gene074354 | MS.gene045369 | PPI |
| MS.gene028709 | MS.gene045369 | PPI |
| MS.gene75680 | MS.gene045369 | PPI |
| MS.gene028711 | MS.gene045369 | PPI |
| MS.gene77522 | MS.gene045369 | PPI |
| MS.gene045369 | MS.gene31284 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene045369.t1 | MTR_3g084140 | 100.000 | 73 | 0 | 0 | 1 | 73 | 1 | 73 | 6.86e-49 | 148 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene045369.t1 | AT5G42300 | 94.521 | 73 | 4 | 0 | 1 | 73 | 1 | 73 | 1.07e-45 | 140 |
| MS.gene045369.t1 | AT3G45180 | 90.411 | 73 | 7 | 0 | 1 | 73 | 1 | 73 | 2.19e-44 | 137 |
| MS.gene045369.t1 | AT3G45180 | 90.411 | 73 | 7 | 0 | 1 | 73 | 1 | 73 | 2.19e-44 | 137 |
Find 22 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATCTTGTCAGCCCTGGTTC+CGG | 0.386948 | 3.4:-78882763 | None:intergenic |
| TGGTGTACCACTTCTGAATT+CGG | 0.396732 | 3.4:-78882785 | None:intergenic |
| TGGCTGCTCAGACCGGAACC+AGG | 0.440186 | 3.4:+78882751 | MS.gene045369:CDS |
| GTTGTTCTGAACGATCGATT+GGG | 0.442659 | 3.4:+78882669 | MS.gene045369:CDS |
| AATTCGGATCTTGTCAGCCC+TGG | 0.475569 | 3.4:-78882769 | None:intergenic |
| GGTTGTTCTGAACGATCGAT+TGG | 0.506700 | 3.4:+78882668 | MS.gene045369:CDS |
| GAACGATCGATTGGGGAAGA+AGG | 0.524636 | 3.4:+78882677 | MS.gene045369:CDS |
| AAGCTGGTGGCTGCTCAGAC+CGG | 0.532945 | 3.4:+78882744 | MS.gene045369:CDS |
| AGTGATGTGATCCTTGTAGA+TGG | 0.540415 | 3.4:-78882805 | None:intergenic |
| GTTCAAGACCCATGCCATCA+TGG | 0.543744 | 3.4:-78882845 | None:intergenic |
| GATCGGTGATTTGAAGAAGC+TGG | 0.556890 | 3.4:+78882728 | MS.gene045369:CDS |
| TACAAGGATCACATCACTCT+CGG | 0.558076 | 3.4:+78882810 | MS.gene045369:CDS |
| GGCTGCTCAGACCGGAACCA+GGG | 0.582375 | 3.4:+78882752 | MS.gene045369:CDS |
| TACGAGATCCATGATGGCAT+GGG | 0.593857 | 3.4:+78882837 | MS.gene045369:CDS |
| CTACGCTACGAAGATGATTG+AGG | 0.594624 | 3.4:+78882647 | None:intergenic |
| TTACGAGATCCATGATGGCA+TGG | 0.599697 | 3.4:+78882836 | MS.gene045369:CDS |
| TTGTTCTGAACGATCGATTG+GGG | 0.606269 | 3.4:+78882670 | MS.gene045369:CDS |
| GGTGATTACGAGATCCATGA+TGG | 0.610211 | 3.4:+78882831 | MS.gene045369:CDS |
| GAAGTGGTACACCATCTACA+AGG | 0.616793 | 3.4:+78882794 | MS.gene045369:CDS |
| ACAAGATCCGAATTCAGAAG+TGG | 0.640248 | 3.4:+78882778 | MS.gene045369:CDS |
| TGCAACTCAGATGACACGAT+CGG | 0.661294 | 3.4:+78882711 | MS.gene045369:CDS |
| CGGTGATTTGAAGAAGCTGG+TGG | 0.708874 | 3.4:+78882731 | MS.gene045369:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACAAGATCCGAATTCAGAAG+TGG | + | chr3.4:78882778-78882797 | MS.gene045369:CDS | 40.0% | |
| AGTGATGTGATCCTTGTAGA+TGG | - | chr3.4:78882808-78882827 | None:intergenic | 40.0% | |
| TACAAGGATCACATCACTCT+CGG | + | chr3.4:78882810-78882829 | MS.gene045369:CDS | 40.0% | |
| TGGTGTACCACTTCTGAATT+CGG | - | chr3.4:78882788-78882807 | None:intergenic | 40.0% | |
| ! | GTTGTTCTGAACGATCGATT+GGG | + | chr3.4:78882669-78882688 | MS.gene045369:CDS | 40.0% |
| ! | TTGTTCTGAACGATCGATTG+GGG | + | chr3.4:78882670-78882689 | MS.gene045369:CDS | 40.0% |
| GAAGTGGTACACCATCTACA+AGG | + | chr3.4:78882794-78882813 | MS.gene045369:CDS | 45.0% | |
| GATCGGTGATTTGAAGAAGC+TGG | + | chr3.4:78882728-78882747 | MS.gene045369:CDS | 45.0% | |
| GGTGATTACGAGATCCATGA+TGG | + | chr3.4:78882831-78882850 | MS.gene045369:CDS | 45.0% | |
| TACGAGATCCATGATGGCAT+GGG | + | chr3.4:78882837-78882856 | MS.gene045369:CDS | 45.0% | |
| TGCAACTCAGATGACACGAT+CGG | + | chr3.4:78882711-78882730 | MS.gene045369:CDS | 45.0% | |
| TTACGAGATCCATGATGGCA+TGG | + | chr3.4:78882836-78882855 | MS.gene045369:CDS | 45.0% | |
| ! | GGTTGTTCTGAACGATCGAT+TGG | + | chr3.4:78882668-78882687 | MS.gene045369:CDS | 45.0% |
| AATTCGGATCTTGTCAGCCC+TGG | - | chr3.4:78882772-78882791 | None:intergenic | 50.0% | |
| CGGTGATTTGAAGAAGCTGG+TGG | + | chr3.4:78882731-78882750 | MS.gene045369:CDS | 50.0% | |
| GAACGATCGATTGGGGAAGA+AGG | + | chr3.4:78882677-78882696 | MS.gene045369:CDS | 50.0% | |
| GTTCAAGACCCATGCCATCA+TGG | - | chr3.4:78882848-78882867 | None:intergenic | 50.0% | |
| GATCTTGTCAGCCCTGGTTC+CGG | - | chr3.4:78882766-78882785 | None:intergenic | 55.0% | |
| AAGCTGGTGGCTGCTCAGAC+CGG | + | chr3.4:78882744-78882763 | MS.gene045369:CDS | 60.0% | |
| GGCTGCTCAGACCGGAACCA+GGG | + | chr3.4:78882752-78882771 | MS.gene045369:CDS | 65.0% | |
| TGGCTGCTCAGACCGGAACC+AGG | + | chr3.4:78882751-78882770 | MS.gene045369:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 78882660 | 78882881 | 78882660 | ID=MS.gene045369 |
| chr3.4 | mRNA | 78882660 | 78882881 | 78882660 | ID=MS.gene045369.t1;Parent=MS.gene045369 |
| chr3.4 | exon | 78882660 | 78882881 | 78882660 | ID=MS.gene045369.t1.exon1;Parent=MS.gene045369.t1 |
| chr3.4 | CDS | 78882660 | 78882881 | 78882660 | ID=cds.MS.gene045369.t1;Parent=MS.gene045369.t1 |
| Gene Sequence |
| Protein sequence |
