Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79884.t1 | XP_003601666.1 | 100 | 73 | 0 | 0 | 1 | 73 | 1 | 73 | 5.70E-34 | 152.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79884.t1 | Q9FGZ9 | 94.5 | 73 | 4 | 0 | 1 | 73 | 1 | 73 | 3.7e-34 | 144.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79884.t1 | G7JAQ0 | 100.0 | 73 | 0 | 0 | 1 | 73 | 1 | 73 | 4.1e-34 | 152.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene79884 | 0.862711 | 4.18E-64 | -1.69E-46 |
| MS.gene049255 | MS.gene79884 | 0.815013 | 1.15E-51 | -1.69E-46 |
| MS.gene049378 | MS.gene79884 | 0.808105 | 3.65E-50 | -1.69E-46 |
| MS.gene049382 | MS.gene79884 | 0.832767 | 7.84E-56 | -1.69E-46 |
| MS.gene049424 | MS.gene79884 | 0.825778 | 3.89E-54 | -1.69E-46 |
| MS.gene049653 | MS.gene79884 | 0.806754 | 7.05E-50 | -1.69E-46 |
| MS.gene049756 | MS.gene79884 | 0.814788 | 1.29E-51 | -1.69E-46 |
| MS.gene04984 | MS.gene79884 | 0.800625 | 1.32E-48 | -1.69E-46 |
| MS.gene050277 | MS.gene79884 | 0.814813 | 1.27E-51 | -1.69E-46 |
| MS.gene050605 | MS.gene79884 | 0.805114 | 1.56E-49 | -1.69E-46 |
| MS.gene050623 | MS.gene79884 | -0.802148 | 6.44E-49 | -1.69E-46 |
| MS.gene050719 | MS.gene79884 | 0.818104 | 2.33E-52 | -1.69E-46 |
| MS.gene050721 | MS.gene79884 | 0.820437 | 6.86E-53 | -1.69E-46 |
| MS.gene050724 | MS.gene79884 | 0.827593 | 1.44E-54 | -1.69E-46 |
| MS.gene050780 | MS.gene79884 | 0.809095 | 2.24E-50 | -1.69E-46 |
| MS.gene050815 | MS.gene79884 | 0.837242 | 5.84E-57 | -1.69E-46 |
| MS.gene050987 | MS.gene79884 | 0.802028 | 6.81E-49 | -1.69E-46 |
| MS.gene051243 | MS.gene79884 | 0.872124 | 4.05E-67 | -1.69E-46 |
| MS.gene051245 | MS.gene79884 | 0.844685 | 6.49E-59 | -1.69E-46 |
| MS.gene051328 | MS.gene79884 | 0.828708 | 7.74E-55 | -1.69E-46 |
| MS.gene051349 | MS.gene79884 | 0.8362 | 1.08E-56 | -1.69E-46 |
| MS.gene05134 | MS.gene79884 | 0.820129 | 8.07E-53 | -1.69E-46 |
| MS.gene051370 | MS.gene79884 | 0.800366 | 1.49E-48 | -1.69E-46 |
| MS.gene051452 | MS.gene79884 | 0.876539 | 1.29E-68 | -1.69E-46 |
| MS.gene051757 | MS.gene79884 | 0.818735 | 1.68E-52 | -1.69E-46 |
| MS.gene052012 | MS.gene79884 | 0.816733 | 4.75E-52 | -1.69E-46 |
| MS.gene052176 | MS.gene79884 | 0.823291 | 1.50E-53 | -1.69E-46 |
| MS.gene052286 | MS.gene79884 | 0.81768 | 2.91E-52 | -1.69E-46 |
| MS.gene052302 | MS.gene79884 | 0.812065 | 5.11E-51 | -1.69E-46 |
| MS.gene052376 | MS.gene79884 | 0.825403 | 4.78E-54 | -1.69E-46 |
| MS.gene052426 | MS.gene79884 | 0.853219 | 2.76E-61 | -1.69E-46 |
| MS.gene052863 | MS.gene79884 | 0.819158 | 1.34E-52 | -1.69E-46 |
| MS.gene052896 | MS.gene79884 | 0.812401 | 4.32E-51 | -1.69E-46 |
| MS.gene053006 | MS.gene79884 | 0.808841 | 2.54E-50 | -1.69E-46 |
| MS.gene053008 | MS.gene79884 | 0.862448 | 5.03E-64 | -1.69E-46 |
| MS.gene053216 | MS.gene79884 | 0.800443 | 1.44E-48 | -1.69E-46 |
| MS.gene054472 | MS.gene79884 | 0.819768 | 9.76E-53 | -1.69E-46 |
| MS.gene054930 | MS.gene79884 | 0.800198 | 1.61E-48 | -1.69E-46 |
| MS.gene05510 | MS.gene79884 | 0.844523 | 7.17E-59 | -1.69E-46 |
| MS.gene055162 | MS.gene79884 | 0.803358 | 3.62E-49 | -1.69E-46 |
| MS.gene05517 | MS.gene79884 | 0.824873 | 6.38E-54 | -1.69E-46 |
| MS.gene055623 | MS.gene79884 | 0.84125 | 5.33E-58 | -1.69E-46 |
| MS.gene055701 | MS.gene79884 | 0.820419 | 6.93E-53 | -1.69E-46 |
| MS.gene055818 | MS.gene79884 | 0.813414 | 2.59E-51 | -1.69E-46 |
| MS.gene056069 | MS.gene79884 | -0.800234 | 1.59E-48 | -1.69E-46 |
| MS.gene056506 | MS.gene79884 | 0.829956 | 3.85E-55 | -1.69E-46 |
| MS.gene056631 | MS.gene79884 | 0.813384 | 2.63E-51 | -1.69E-46 |
| MS.gene056760 | MS.gene79884 | 0.833605 | 4.84E-56 | -1.69E-46 |
| MS.gene057140 | MS.gene79884 | 0.86713 | 1.72E-65 | -1.69E-46 |
| MS.gene057234 | MS.gene79884 | 0.809221 | 2.11E-50 | -1.69E-46 |
| MS.gene057392 | MS.gene79884 | 0.809447 | 1.88E-50 | -1.69E-46 |
| MS.gene057693 | MS.gene79884 | 0.830952 | 2.20E-55 | -1.69E-46 |
| MS.gene057754 | MS.gene79884 | 0.805448 | 1.33E-49 | -1.69E-46 |
| MS.gene05795 | MS.gene79884 | 0.814212 | 1.73E-51 | -1.69E-46 |
| MS.gene058055 | MS.gene79884 | 0.801914 | 7.19E-49 | -1.69E-46 |
| MS.gene058084 | MS.gene79884 | 0.80669 | 7.28E-50 | -1.69E-46 |
| MS.gene058146 | MS.gene79884 | 0.835764 | 1.39E-56 | -1.69E-46 |
| MS.gene058182 | MS.gene79884 | 0.812987 | 3.21E-51 | -1.69E-46 |
| MS.gene058238 | MS.gene79884 | 0.801715 | 7.90E-49 | -1.69E-46 |
| MS.gene058399 | MS.gene79884 | 0.827186 | 1.80E-54 | -1.69E-46 |
| MS.gene058595 | MS.gene79884 | 0.840566 | 8.05E-58 | -1.69E-46 |
| MS.gene058906 | MS.gene79884 | 0.842798 | 2.08E-58 | -1.69E-46 |
| MS.gene058979 | MS.gene79884 | 0.811081 | 8.37E-51 | -1.69E-46 |
| MS.gene059249 | MS.gene79884 | 0.811232 | 7.76E-51 | -1.69E-46 |
| MS.gene059830 | MS.gene79884 | 0.841417 | 4.81E-58 | -1.69E-46 |
| MS.gene059842 | MS.gene79884 | 0.808508 | 2.99E-50 | -1.69E-46 |
| MS.gene059898 | MS.gene79884 | 0.82726 | 1.73E-54 | -1.69E-46 |
| MS.gene060310 | MS.gene79884 | 0.823306 | 1.49E-53 | -1.69E-46 |
| MS.gene060322 | MS.gene79884 | 0.862984 | 3.44E-64 | -1.69E-46 |
| MS.gene060586 | MS.gene79884 | 0.808667 | 2.77E-50 | -1.69E-46 |
| MS.gene060683 | MS.gene79884 | 0.849235 | 3.68E-60 | -1.69E-46 |
| MS.gene060685 | MS.gene79884 | 0.851462 | 8.74E-61 | -1.69E-46 |
| MS.gene060802 | MS.gene79884 | 0.800749 | 1.25E-48 | -1.69E-46 |
| MS.gene060803 | MS.gene79884 | 0.838358 | 3.02E-57 | -1.69E-46 |
| MS.gene060942 | MS.gene79884 | 0.80296 | 4.38E-49 | -1.69E-46 |
| MS.gene060998 | MS.gene79884 | 0.859014 | 5.54E-63 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene79884 | MS.gene045369 | PPI |
| MS.gene79884 | MS.gene028709 | PPI |
| MS.gene074354 | MS.gene79884 | PPI |
| MS.gene028709 | MS.gene79884 | PPI |
| MS.gene79884 | MS.gene31284 | PPI |
| MS.gene75680 | MS.gene79884 | PPI |
| MS.gene79884 | MS.gene028711 | PPI |
| MS.gene79884 | MS.gene058936 | PPI |
| MS.gene79884 | MS.gene77522 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79884.t1 | MTR_3g084140 | 100.000 | 73 | 0 | 0 | 1 | 73 | 1 | 73 | 6.86e-49 | 148 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79884.t1 | AT5G42300 | 94.521 | 73 | 4 | 0 | 1 | 73 | 1 | 73 | 1.07e-45 | 140 |
| MS.gene79884.t1 | AT3G45180 | 90.411 | 73 | 7 | 0 | 1 | 73 | 1 | 73 | 2.19e-44 | 137 |
| MS.gene79884.t1 | AT3G45180 | 90.411 | 73 | 7 | 0 | 1 | 73 | 1 | 73 | 2.19e-44 | 137 |
Find 22 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATCTTGTCAGCCCTGGTTC+CGG | 0.386948 | 3.1:+65492442 | None:intergenic |
| TGGTGTACCACTTCTGAATT+CGG | 0.396732 | 3.1:+65492420 | None:intergenic |
| TGGCTGCTCAGACCGGAACC+AGG | 0.440186 | 3.1:-65492454 | MS.gene79884:CDS |
| GTTGTTCTGAACGATCGATT+GGG | 0.442659 | 3.1:-65492536 | MS.gene79884:CDS |
| AATTCGGATCTTGTCAGCCC+TGG | 0.475569 | 3.1:+65492436 | None:intergenic |
| GGTTGTTCTGAACGATCGAT+TGG | 0.506700 | 3.1:-65492537 | MS.gene79884:CDS |
| GAACGATCGATTGGGGAAGA+AGG | 0.524636 | 3.1:-65492528 | MS.gene79884:CDS |
| AAGCTGGTGGCTGCTCAGAC+CGG | 0.532945 | 3.1:-65492461 | MS.gene79884:CDS |
| AGTGATGTGATCCTTGTAGA+TGG | 0.540415 | 3.1:+65492400 | None:intergenic |
| GTTCAAGACCCATGCCATCA+TGG | 0.543744 | 3.1:+65492360 | None:intergenic |
| GATCGGTGATTTGAAGAAGC+TGG | 0.556890 | 3.1:-65492477 | MS.gene79884:CDS |
| TACAAGGATCACATCACTCT+CGG | 0.558076 | 3.1:-65492395 | MS.gene79884:CDS |
| GGCTGCTCAGACCGGAACCA+GGG | 0.582375 | 3.1:-65492453 | MS.gene79884:CDS |
| TACGAGATCCATGATGGCAT+GGG | 0.593857 | 3.1:-65492368 | MS.gene79884:CDS |
| CTACGCTACGAAGATGATTG+AGG | 0.594624 | 3.1:-65492558 | None:intergenic |
| TTACGAGATCCATGATGGCA+TGG | 0.599697 | 3.1:-65492369 | MS.gene79884:CDS |
| TTGTTCTGAACGATCGATTG+GGG | 0.606269 | 3.1:-65492535 | MS.gene79884:CDS |
| GGTGATTACGAGATCCATGA+TGG | 0.610211 | 3.1:-65492374 | MS.gene79884:CDS |
| GAAGTGGTACACCATCTACA+AGG | 0.616793 | 3.1:-65492411 | MS.gene79884:CDS |
| ACAAGATCCGAATTCAGAAG+TGG | 0.640248 | 3.1:-65492427 | MS.gene79884:CDS |
| TGCAACTCAGATGACACGAT+CGG | 0.661294 | 3.1:-65492494 | MS.gene79884:CDS |
| CGGTGATTTGAAGAAGCTGG+TGG | 0.708874 | 3.1:-65492474 | MS.gene79884:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACAAGATCCGAATTCAGAAG+TGG | - | chr3.1:65492464-65492483 | MS.gene79884:CDS | 40.0% | |
| AGTGATGTGATCCTTGTAGA+TGG | + | chr3.1:65492494-65492513 | None:intergenic | 40.0% | |
| TACAAGGATCACATCACTCT+CGG | - | chr3.1:65492496-65492515 | MS.gene79884:CDS | 40.0% | |
| TGGTGTACCACTTCTGAATT+CGG | + | chr3.1:65492474-65492493 | None:intergenic | 40.0% | |
| ! | GTTGTTCTGAACGATCGATT+GGG | - | chr3.1:65492355-65492374 | MS.gene79884:CDS | 40.0% |
| ! | TTGTTCTGAACGATCGATTG+GGG | - | chr3.1:65492356-65492375 | MS.gene79884:CDS | 40.0% |
| GAAGTGGTACACCATCTACA+AGG | - | chr3.1:65492480-65492499 | MS.gene79884:CDS | 45.0% | |
| GATCGGTGATTTGAAGAAGC+TGG | - | chr3.1:65492414-65492433 | MS.gene79884:CDS | 45.0% | |
| GGTGATTACGAGATCCATGA+TGG | - | chr3.1:65492517-65492536 | MS.gene79884:CDS | 45.0% | |
| TACGAGATCCATGATGGCAT+GGG | - | chr3.1:65492523-65492542 | MS.gene79884:CDS | 45.0% | |
| TGCAACTCAGATGACACGAT+CGG | - | chr3.1:65492397-65492416 | MS.gene79884:CDS | 45.0% | |
| TTACGAGATCCATGATGGCA+TGG | - | chr3.1:65492522-65492541 | MS.gene79884:CDS | 45.0% | |
| ! | GGTTGTTCTGAACGATCGAT+TGG | - | chr3.1:65492354-65492373 | MS.gene79884:CDS | 45.0% |
| AATTCGGATCTTGTCAGCCC+TGG | + | chr3.1:65492458-65492477 | None:intergenic | 50.0% | |
| CGGTGATTTGAAGAAGCTGG+TGG | - | chr3.1:65492417-65492436 | MS.gene79884:CDS | 50.0% | |
| GAACGATCGATTGGGGAAGA+AGG | - | chr3.1:65492363-65492382 | MS.gene79884:CDS | 50.0% | |
| GTTCAAGACCCATGCCATCA+TGG | + | chr3.1:65492534-65492553 | None:intergenic | 50.0% | |
| GATCTTGTCAGCCCTGGTTC+CGG | + | chr3.1:65492452-65492471 | None:intergenic | 55.0% | |
| AAGCTGGTGGCTGCTCAGAC+CGG | - | chr3.1:65492430-65492449 | MS.gene79884:CDS | 60.0% | |
| GGCTGCTCAGACCGGAACCA+GGG | - | chr3.1:65492438-65492457 | MS.gene79884:CDS | 65.0% | |
| TGGCTGCTCAGACCGGAACC+AGG | - | chr3.1:65492437-65492456 | MS.gene79884:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 65492346 | 65492567 | 65492346 | ID=MS.gene79884 |
| chr3.1 | mRNA | 65492346 | 65492567 | 65492346 | ID=MS.gene79884.t1;Parent=MS.gene79884 |
| chr3.1 | exon | 65492346 | 65492567 | 65492346 | ID=MS.gene79884.t1.exon1;Parent=MS.gene79884.t1 |
| chr3.1 | CDS | 65492346 | 65492567 | 65492346 | ID=cds.MS.gene79884.t1;Parent=MS.gene79884.t1 |
| Gene Sequence |
| Protein sequence |
