AlfalfaGEDB | MS.gene75680

M. sativa cultivar XinJiangDaYe / MS.gene75680

KEGG: K13113
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00240.26

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75680.t1	XP_003601666.1	100	73	0	0	1	73	1	73	5.70E-34	152.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75680.t1	Q9FGZ9	94.5	73	4	0	1	73	1	73	3.7e-34	144.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75680.t1	G7JAQ0	100.0	73	0	0	1	73	1	73	4.1e-34	152.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene75680	0.816598	5.09E-52	-1.69E-46
MS.gene049255	MS.gene75680	0.819667	1.03E-52	-1.69E-46
MS.gene049424	MS.gene75680	0.815049	1.13E-51	-1.69E-46
MS.gene049653	MS.gene75680	0.848559	5.67E-60	-1.69E-46
MS.gene050277	MS.gene75680	0.830026	3.70E-55	-1.69E-46
MS.gene050384	MS.gene75680	0.803704	3.07E-49	-1.69E-46
MS.gene050386	MS.gene75680	0.80839	3.17E-50	-1.69E-46
MS.gene050437	MS.gene75680	0.826514	2.60E-54	-1.69E-46
MS.gene050689	MS.gene75680	0.808778	2.62E-50	-1.69E-46
MS.gene050724	MS.gene75680	0.80219	6.31E-49	-1.69E-46
MS.gene050807	MS.gene75680	0.804072	2.57E-49	-1.69E-46
MS.gene050815	MS.gene75680	0.835231	1.89E-56	-1.69E-46
MS.gene050987	MS.gene75680	0.831855	1.32E-55	-1.69E-46
MS.gene051241	MS.gene75680	0.8202	7.78E-53	-1.69E-46
MS.gene051243	MS.gene75680	0.859048	5.41E-63	-1.69E-46
MS.gene051245	MS.gene75680	0.847441	1.15E-59	-1.69E-46
MS.gene051328	MS.gene75680	0.841457	4.70E-58	-1.69E-46
MS.gene051349	MS.gene75680	0.811747	6.00E-51	-1.69E-46
MS.gene05134	MS.gene75680	0.844045	9.64E-59	-1.69E-46
MS.gene051452	MS.gene75680	0.840706	7.40E-58	-1.69E-46
MS.gene051577	MS.gene75680	0.801195	1.01E-48	-1.69E-46
MS.gene051757	MS.gene75680	0.811711	6.11E-51	-1.69E-46
MS.gene052012	MS.gene75680	0.805495	1.30E-49	-1.69E-46
MS.gene052176	MS.gene75680	0.802068	6.69E-49	-1.69E-46
MS.gene052286	MS.gene75680	0.824704	6.99E-54	-1.69E-46
MS.gene052302	MS.gene75680	0.802035	6.79E-49	-1.69E-46
MS.gene052376	MS.gene75680	0.809422	1.91E-50	-1.69E-46
MS.gene052426	MS.gene75680	0.835643	1.49E-56	-1.69E-46
MS.gene052954	MS.gene75680	0.822745	2.01E-53	-1.69E-46
MS.gene053008	MS.gene75680	0.83779	4.22E-57	-1.69E-46
MS.gene054657	MS.gene75680	0.845519	3.86E-59	-1.69E-46
MS.gene05510	MS.gene75680	0.834369	3.12E-56	-1.69E-46
MS.gene05517	MS.gene75680	0.818314	2.09E-52	-1.69E-46
MS.gene055512	MS.gene75680	0.816425	5.57E-52	-1.69E-46
MS.gene055623	MS.gene75680	0.86056	1.90E-63	-1.69E-46
MS.gene055818	MS.gene75680	0.804594	2.00E-49	-1.69E-46
MS.gene056631	MS.gene75680	0.825889	3.66E-54	-1.69E-46
MS.gene056760	MS.gene75680	0.856003	4.31E-62	-1.69E-46
MS.gene057057	MS.gene75680	0.801543	8.57E-49	-1.69E-46
MS.gene057140	MS.gene75680	0.852213	5.35E-61	-1.69E-46
MS.gene057234	MS.gene75680	0.816333	5.84E-52	-1.69E-46
MS.gene057392	MS.gene75680	0.826812	2.21E-54	-1.69E-46
MS.gene057693	MS.gene75680	0.803147	4.00E-49	-1.69E-46
MS.gene05795	MS.gene75680	0.837077	6.43E-57	-1.69E-46
MS.gene058055	MS.gene75680	0.808055	3.74E-50	-1.69E-46
MS.gene058088	MS.gene75680	0.807969	3.90E-50	-1.69E-46
MS.gene058146	MS.gene75680	0.806804	6.88E-50	-1.69E-46
MS.gene058238	MS.gene75680	0.819898	9.12E-53	-1.69E-46
MS.gene058595	MS.gene75680	0.811534	6.67E-51	-1.69E-46
MS.gene058906	MS.gene75680	0.846398	2.23E-59	-1.69E-46
MS.gene059317	MS.gene75680	0.826211	3.07E-54	-1.69E-46
MS.gene05979	MS.gene75680	0.807593	4.69E-50	-1.69E-46
MS.gene059830	MS.gene75680	0.824606	7.37E-54	-1.69E-46
MS.gene059842	MS.gene75680	0.82578	3.89E-54	-1.69E-46
MS.gene059898	MS.gene75680	0.803753	3.00E-49	-1.69E-46
MS.gene060297	MS.gene75680	0.814687	1.36E-51	-1.69E-46
MS.gene060322	MS.gene75680	0.841914	3.56E-58	-1.69E-46
MS.gene060683	MS.gene75680	0.812186	4.81E-51	-1.69E-46
MS.gene060685	MS.gene75680	0.811288	7.55E-51	-1.69E-46
MS.gene060803	MS.gene75680	0.803843	2.87E-49	-1.69E-46
MS.gene060998	MS.gene75680	0.820616	6.24E-53	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene75680	MS.gene045369	PPI
MS.gene75680	MS.gene028709	PPI
MS.gene75680	MS.gene79884	PPI
MS.gene75680	MS.gene31284	PPI
MS.gene074354	MS.gene75680	PPI
MS.gene75680	MS.gene028711	PPI
MS.gene75680	MS.gene77522	PPI
MS.gene75680	MS.gene058936	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75680.t1	MTR_3g084140	100.000	73	0	0	1	73	1	73	6.86e-49	148

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75680.t1	AT5G42300	94.521	73	4	1	73	1	73	1.07e-45	140
MS.gene75680.t1	AT3G45180	90.411	73	7	1	73	1	73	2.19e-44	137
MS.gene75680.t1	AT3G45180	90.411	73	7	1	73	1	73	2.19e-44	137

Find 22 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GATCTTGTCAGCCCTGGTTC+CGG	0.386948	3.3:-70561219	None:intergenic
TGGTGTACCACTTCTGAATT+CGG	0.396732	3.3:-70561241	None:intergenic
TGGCTGCTCAGACCGGAACC+AGG	0.440186	3.3:+70561207	MS.gene75680:CDS
GTTGTTCTGAACGATCGATT+GGG	0.442659	3.3:+70561125	MS.gene75680:CDS
AATTCGGATCTTGTCAGCCC+TGG	0.475569	3.3:-70561225	None:intergenic
GGTTGTTCTGAACGATCGAT+TGG	0.506700	3.3:+70561124	MS.gene75680:CDS
GAACGATCGATTGGGGAAGA+AGG	0.524636	3.3:+70561133	MS.gene75680:CDS
AAGCTGGTGGCTGCTCAGAC+CGG	0.532945	3.3:+70561200	MS.gene75680:CDS
AGTGATGTGATCCTTGTAGA+TGG	0.540415	3.3:-70561261	None:intergenic
GTTCAAGACCCATGCCATCA+TGG	0.543744	3.3:-70561301	None:intergenic
GATCGGTGATTTGAAGAAGC+TGG	0.556890	3.3:+70561184	MS.gene75680:CDS
TACAAGGATCACATCACTCT+CGG	0.558076	3.3:+70561266	MS.gene75680:CDS
GGCTGCTCAGACCGGAACCA+GGG	0.582375	3.3:+70561208	MS.gene75680:CDS
TACGAGATCCATGATGGCAT+GGG	0.593857	3.3:+70561293	MS.gene75680:CDS
CTACGCTACGAAGATGATTG+AGG	0.594624	3.3:+70561103	None:intergenic
TTACGAGATCCATGATGGCA+TGG	0.599697	3.3:+70561292	MS.gene75680:CDS
TTGTTCTGAACGATCGATTG+GGG	0.606269	3.3:+70561126	MS.gene75680:CDS
GGTGATTACGAGATCCATGA+TGG	0.610211	3.3:+70561287	MS.gene75680:CDS
GAAGTGGTACACCATCTACA+AGG	0.616793	3.3:+70561250	MS.gene75680:CDS
ACAAGATCCGAATTCAGAAG+TGG	0.640248	3.3:+70561234	MS.gene75680:CDS
TGCAACTCAGATGACACGAT+CGG	0.661294	3.3:+70561167	MS.gene75680:CDS
CGGTGATTTGAAGAAGCTGG+TGG	0.708874	3.3:+70561187	MS.gene75680:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ACAAGATCCGAATTCAGAAG+TGG	+	chr3.3:70561234-70561253	MS.gene75680:CDS	40.0%
	AGTGATGTGATCCTTGTAGA+TGG	-	chr3.3:70561264-70561283	None:intergenic	40.0%
	TACAAGGATCACATCACTCT+CGG	+	chr3.3:70561266-70561285	MS.gene75680:CDS	40.0%
	TGGTGTACCACTTCTGAATT+CGG	-	chr3.3:70561244-70561263	None:intergenic	40.0%
!	GTTGTTCTGAACGATCGATT+GGG	+	chr3.3:70561125-70561144	MS.gene75680:CDS	40.0%
!	TTGTTCTGAACGATCGATTG+GGG	+	chr3.3:70561126-70561145	MS.gene75680:CDS	40.0%
	GAAGTGGTACACCATCTACA+AGG	+	chr3.3:70561250-70561269	MS.gene75680:CDS	45.0%
	GATCGGTGATTTGAAGAAGC+TGG	+	chr3.3:70561184-70561203	MS.gene75680:CDS	45.0%
	GGTGATTACGAGATCCATGA+TGG	+	chr3.3:70561287-70561306	MS.gene75680:CDS	45.0%
	TACGAGATCCATGATGGCAT+GGG	+	chr3.3:70561293-70561312	MS.gene75680:CDS	45.0%
	TGCAACTCAGATGACACGAT+CGG	+	chr3.3:70561167-70561186	MS.gene75680:CDS	45.0%
	TTACGAGATCCATGATGGCA+TGG	+	chr3.3:70561292-70561311	MS.gene75680:CDS	45.0%
!	GGTTGTTCTGAACGATCGAT+TGG	+	chr3.3:70561124-70561143	MS.gene75680:CDS	45.0%
	AATTCGGATCTTGTCAGCCC+TGG	-	chr3.3:70561228-70561247	None:intergenic	50.0%
	CGGTGATTTGAAGAAGCTGG+TGG	+	chr3.3:70561187-70561206	MS.gene75680:CDS	50.0%
	GAACGATCGATTGGGGAAGA+AGG	+	chr3.3:70561133-70561152	MS.gene75680:CDS	50.0%
	GTTCAAGACCCATGCCATCA+TGG	-	chr3.3:70561304-70561323	None:intergenic	50.0%
	GATCTTGTCAGCCCTGGTTC+CGG	-	chr3.3:70561222-70561241	None:intergenic	55.0%
	AAGCTGGTGGCTGCTCAGAC+CGG	+	chr3.3:70561200-70561219	MS.gene75680:CDS	60.0%
	GGCTGCTCAGACCGGAACCA+GGG	+	chr3.3:70561208-70561227	MS.gene75680:CDS	65.0%
	TGGCTGCTCAGACCGGAACC+AGG	+	chr3.3:70561207-70561226	MS.gene75680:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	70561116	70561337	70561116	ID=MS.gene75680
chr3.3	mRNA	70561116	70561337	70561116	ID=MS.gene75680.t1;Parent=MS.gene75680
chr3.3	exon	70561116	70561337	70561116	ID=MS.gene75680.t1.exon1;Parent=MS.gene75680.t1
chr3.3	CDS	70561116	70561337	70561116	ID=cds.MS.gene75680.t1;Parent=MS.gene75680.t1

Gene Sequence	>MS.gene75680 ATGATTGAGGTTGTTCTGAACGATCGATTGGGGAAGAAGGTTCGCGTGAAGTGCAACTCAGATGACACGATCGGTGATTTGAAGAAGCTGGTGGCTGCTCAGACCGGAACCAGGGCTGACAAGATCCGAATTCAGAAGTGGTACACCATCTACAAGGATCACATCACTCTCGGTGATTACGAGATCCATGATGGCATGGGTCTTGAACTCTACTACAACTAA
Protein sequence	>MS.gene75680.t1 MIEVVLNDRLGKKVRVKCNSDDTIGDLKKLVAAQTGTRADKIRIQKWYTIYKDHITLGDYEIHDGMGLELYYN