Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77522.t1 | XP_003601666.1 | 100 | 73 | 0 | 0 | 1 | 73 | 1 | 73 | 5.70E-34 | 152.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77522.t1 | Q9FGZ9 | 94.5 | 73 | 4 | 0 | 1 | 73 | 1 | 73 | 3.7e-34 | 144.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77522.t1 | G7JAQ0 | 100.0 | 73 | 0 | 0 | 1 | 73 | 1 | 73 | 4.1e-34 | 152.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053006 | MS.gene77522 | 0.808619 | 2.83E-50 | -1.69E-46 |
| MS.gene053008 | MS.gene77522 | 0.815589 | 8.56E-52 | -1.69E-46 |
| MS.gene05415 | MS.gene77522 | 0.829903 | 3.97E-55 | -1.69E-46 |
| MS.gene054657 | MS.gene77522 | 0.801345 | 9.41E-49 | -1.69E-46 |
| MS.gene05510 | MS.gene77522 | 0.812511 | 4.09E-51 | -1.69E-46 |
| MS.gene055623 | MS.gene77522 | 0.812024 | 5.22E-51 | -1.69E-46 |
| MS.gene055818 | MS.gene77522 | 0.821915 | 3.13E-53 | -1.69E-46 |
| MS.gene057060 | MS.gene77522 | 0.802489 | 5.48E-49 | -1.69E-46 |
| MS.gene057392 | MS.gene77522 | 0.805609 | 1.23E-49 | -1.69E-46 |
| MS.gene057693 | MS.gene77522 | 0.809089 | 2.25E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene77522 | 0.810138 | 1.34E-50 | -1.69E-46 |
| MS.gene060572 | MS.gene77522 | 0.822557 | 2.22E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene77522 | MS.gene045369 | PPI |
| MS.gene77522 | MS.gene31284 | PPI |
| MS.gene074354 | MS.gene77522 | PPI |
| MS.gene028709 | MS.gene77522 | PPI |
| MS.gene75680 | MS.gene77522 | PPI |
| MS.gene79884 | MS.gene77522 | PPI |
| MS.gene77522 | MS.gene028711 | PPI |
| MS.gene77522 | MS.gene058936 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77522.t1 | MTR_3g084140 | 100.000 | 73 | 0 | 0 | 1 | 73 | 1 | 73 | 6.86e-49 | 148 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77522.t1 | AT5G42300 | 94.521 | 73 | 4 | 0 | 1 | 73 | 1 | 73 | 1.07e-45 | 140 |
| MS.gene77522.t1 | AT3G45180 | 90.411 | 73 | 7 | 0 | 1 | 73 | 1 | 73 | 2.19e-44 | 137 |
| MS.gene77522.t1 | AT3G45180 | 90.411 | 73 | 7 | 0 | 1 | 73 | 1 | 73 | 2.19e-44 | 137 |
Find 21 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATCTTGTCAGCCCTGGTTC+CGG | 0.386948 | 3.2:-72553818 | None:intergenic |
| TGGTGTACCACTTCTGAATT+CGG | 0.396732 | 3.2:-72553840 | None:intergenic |
| TGGCTGCTCAGACCGGAACC+AGG | 0.440186 | 3.2:+72553806 | MS.gene77522:CDS |
| GTTGTTCTGAACGATCGATT+GGG | 0.442659 | 3.2:+72553724 | MS.gene77522:CDS |
| AATTCGGATCTTGTCAGCCC+TGG | 0.475569 | 3.2:-72553824 | None:intergenic |
| GGTTGTTCTGAACGATCGAT+TGG | 0.506700 | 3.2:+72553723 | MS.gene77522:CDS |
| GAACGATCGATTGGGGAAGA+AGG | 0.524636 | 3.2:+72553732 | MS.gene77522:CDS |
| AAGCTGGTGGCTGCTCAGAC+CGG | 0.532945 | 3.2:+72553799 | MS.gene77522:CDS |
| AGTGATGTGATCCTTGTAGA+TGG | 0.540415 | 3.2:-72553860 | None:intergenic |
| GATCGGTGATTTGAAGAAGC+TGG | 0.556890 | 3.2:+72553783 | MS.gene77522:CDS |
| TACAAGGATCACATCACTCT+CGG | 0.558076 | 3.2:+72553865 | MS.gene77522:CDS |
| GGCTGCTCAGACCGGAACCA+GGG | 0.582375 | 3.2:+72553807 | MS.gene77522:CDS |
| TACGAGATTCATGATGGCAT+GGG | 0.593857 | 3.2:+72553892 | MS.gene77522:CDS |
| CTACGCTACGAAGATGATTG+AGG | 0.594624 | 3.2:+72553702 | None:intergenic |
| TTACGAGATTCATGATGGCA+TGG | 0.597401 | 3.2:+72553891 | MS.gene77522:CDS |
| TTGTTCTGAACGATCGATTG+GGG | 0.606269 | 3.2:+72553725 | MS.gene77522:CDS |
| GAAGTGGTACACCATCTACA+AGG | 0.616793 | 3.2:+72553849 | MS.gene77522:CDS |
| ACAAGATCCGAATTCAGAAG+TGG | 0.640248 | 3.2:+72553833 | MS.gene77522:CDS |
| GGTGATTACGAGATTCATGA+TGG | 0.642859 | 3.2:+72553886 | MS.gene77522:CDS |
| TGCAACTCAGATGACACGAT+CGG | 0.661294 | 3.2:+72553766 | MS.gene77522:CDS |
| CGGTGATTTGAAGAAGCTGG+TGG | 0.708874 | 3.2:+72553786 | MS.gene77522:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACAAGATCCGAATTCAGAAG+TGG | + | chr3.2:72553833-72553852 | MS.gene77522:CDS | 40.0% | |
| AGTGATGTGATCCTTGTAGA+TGG | - | chr3.2:72553863-72553882 | None:intergenic | 40.0% | |
| GGTGATTACGAGATTCATGA+TGG | + | chr3.2:72553886-72553905 | MS.gene77522:CDS | 40.0% | |
| TACAAGGATCACATCACTCT+CGG | + | chr3.2:72553865-72553884 | MS.gene77522:CDS | 40.0% | |
| TACGAGATTCATGATGGCAT+GGG | + | chr3.2:72553892-72553911 | MS.gene77522:CDS | 40.0% | |
| TGGTGTACCACTTCTGAATT+CGG | - | chr3.2:72553843-72553862 | None:intergenic | 40.0% | |
| TTACGAGATTCATGATGGCA+TGG | + | chr3.2:72553891-72553910 | MS.gene77522:CDS | 40.0% | |
| ! | GTTGTTCTGAACGATCGATT+GGG | + | chr3.2:72553724-72553743 | MS.gene77522:CDS | 40.0% |
| ! | TTGTTCTGAACGATCGATTG+GGG | + | chr3.2:72553725-72553744 | MS.gene77522:CDS | 40.0% |
| GAAGTGGTACACCATCTACA+AGG | + | chr3.2:72553849-72553868 | MS.gene77522:CDS | 45.0% | |
| GATCGGTGATTTGAAGAAGC+TGG | + | chr3.2:72553783-72553802 | MS.gene77522:CDS | 45.0% | |
| TGCAACTCAGATGACACGAT+CGG | + | chr3.2:72553766-72553785 | MS.gene77522:CDS | 45.0% | |
| ! | GGTTGTTCTGAACGATCGAT+TGG | + | chr3.2:72553723-72553742 | MS.gene77522:CDS | 45.0% |
| AATTCGGATCTTGTCAGCCC+TGG | - | chr3.2:72553827-72553846 | None:intergenic | 50.0% | |
| CGGTGATTTGAAGAAGCTGG+TGG | + | chr3.2:72553786-72553805 | MS.gene77522:CDS | 50.0% | |
| GAACGATCGATTGGGGAAGA+AGG | + | chr3.2:72553732-72553751 | MS.gene77522:CDS | 50.0% | |
| GATCTTGTCAGCCCTGGTTC+CGG | - | chr3.2:72553821-72553840 | None:intergenic | 55.0% | |
| AAGCTGGTGGCTGCTCAGAC+CGG | + | chr3.2:72553799-72553818 | MS.gene77522:CDS | 60.0% | |
| GGCTGCTCAGACCGGAACCA+GGG | + | chr3.2:72553807-72553826 | MS.gene77522:CDS | 65.0% | |
| TGGCTGCTCAGACCGGAACC+AGG | + | chr3.2:72553806-72553825 | MS.gene77522:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 72553715 | 72553936 | 72553715 | ID=MS.gene77522 |
| chr3.2 | mRNA | 72553715 | 72553936 | 72553715 | ID=MS.gene77522.t1;Parent=MS.gene77522 |
| chr3.2 | exon | 72553715 | 72553936 | 72553715 | ID=MS.gene77522.t1.exon1;Parent=MS.gene77522.t1 |
| chr3.2 | CDS | 72553715 | 72553936 | 72553715 | ID=cds.MS.gene77522.t1;Parent=MS.gene77522.t1 |
| Gene Sequence |
| Protein sequence |
