AlfalfaGEDB | MS.gene40349

M. sativa cultivar XinJiangDaYe / MS.gene40349

KEGG: K03439
GO: GO:0003674 GO:0003824 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0008168 GO:0008173 GO:0008175 GO:0008176 GO:0008757 GO:0009451 GO:0009987 GO:0016070 GO:0016423 GO:0016740 GO:0016741 GO:0034470 GO:0034641 GO:0034660 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0046483 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360
PFAM: PF02390.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40349.t1	XP_003603585.1	99.2	255	2	0	1	255	1	255	7.60E-142	513.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40349.t1	Q8GXB7	79.8	243	46	2	8	249	8	248	2.5e-109	396.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40349.t1	G7J2W7	99.2	255	2	0	1	255	1	255	5.5e-142	513.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene055453	MS.gene40349	0.960632	8.90E-119	-1.69E-46
MS.gene055454	MS.gene40349	0.978609	3.47E-146	-1.69E-46
MS.gene055455	MS.gene40349	0.969041	1.52E-129	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene40349	MS.gene61042	PPI
MS.gene40349	MS.gene50496	PPI
MS.gene018116	MS.gene40349	PPI
MS.gene038492	MS.gene40349	PPI
MS.gene40349	MS.gene048631	PPI
MS.gene40349	MS.gene40390	PPI
MS.gene40349	MS.gene71227	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40349.t1	MTR_3g109360	99.216	255	2	0	1	255	1	255	0.0	528

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40349.t1	AT5G24840	79.835	243	46	2	8	249	8	248	1.12e-146	410

Find 65 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGATTAAGTTTGCCGATAT+TGG	0.221557	3.3:+89525784	MS.gene40349:CDS
TCCGCACCACAGAAACATTT+TGG	0.250656	3.3:-89525948	None:intergenic
ATTCCGATCATCAATGTTTC+TGG	0.252188	3.3:-89525850	None:intergenic
GCCGATATTGGCTGTGGTTT+TGG	0.274610	3.3:+89525796	MS.gene40349:CDS
CCTCGTTTCTAGCAACCTTT+TGG	0.326676	3.3:-89526278	None:intergenic
TAAAATGAGGATCGGGAAAC+AGG	0.368009	3.3:-89526032	None:intergenic
TTCTCTTTAAAATGAGGATC+GGG	0.398927	3.3:-89526039	None:intergenic
GCATACTCATCTAGTAAGAA+TGG	0.400409	3.3:-89526087	None:intergenic
AAACGAGGGACAAACTTTCC+AGG	0.404412	3.3:+89526293	MS.gene40349:CDS
TTGTGCTTTCTCAAAGTGAT+TGG	0.404689	3.3:-89525992	None:intergenic
GGACTGGATGAAATCTTGTT+TGG	0.409104	3.3:+89526167	MS.gene40349:CDS
TCCAAAACCACAGCCAATAT+CGG	0.417152	3.3:-89525797	None:intergenic
TTAAGCTCTGCCACTGAAGA+AGG	0.428496	3.3:+89526255	MS.gene40349:CDS
TGTGCTTTCTCAAAGTGATT+GGG	0.444654	3.3:-89525991	None:intergenic
TCAACATTCAGTAAGTCAAC+AGG	0.452366	3.3:+89525610	MS.gene40349:CDS
GTGATTGGGAATGTACTTCA+TGG	0.454613	3.3:-89525977	None:intergenic
GTTGAATTTCCACCAGTCTC+AGG	0.455044	3.3:-89525592	None:intergenic
TGGTTCTTCTCTTTAAAATG+AGG	0.455252	3.3:-89526045	None:intergenic
CAGTCAAAGGTTCAAACAAC+GGG	0.464877	3.3:-89526197	None:intergenic
TAAAGTGTCCGAGTATGTTA+AGG	0.471708	3.3:+89525885	MS.gene40349:CDS
TCAAGTTCTTTCTCAGTCAA+AGG	0.473439	3.3:-89526210	None:intergenic
ATAACAGATGTGGAAGAACT+TGG	0.476880	3.3:+89526144	MS.gene40349:CDS
GCTCGATAGAAGCGCTTTCG+AGG	0.477207	3.3:-89525637	None:intergenic
TCTTTAAGGGTAGCAAATCC+AGG	0.486768	3.3:+89525919	MS.gene40349:CDS
GAAAGAACTTGAAGCAGATC+CGG	0.487319	3.3:+89526221	MS.gene40349:CDS
TTCCGATCATCAATGTTTCT+GGG	0.488618	3.3:-89525849	None:intergenic
AGAGAAGAACCATCGCCGTC+GGG	0.492041	3.3:+89526056	MS.gene40349:CDS
GAGGGAATAGTCAACATGAC+TGG	0.493842	3.3:-89525713	None:intergenic
CTTCTCTTTAAAATGAGGAT+CGG	0.494124	3.3:-89526040	None:intergenic
CATACTCATCTAGTAAGAAT+GGG	0.503167	3.3:-89526086	None:intergenic
TGCCACTGAAGAAGGCCAAA+AGG	0.507812	3.3:+89526263	MS.gene40349:CDS
TCAACATGACTGGGTGATAA+CGG	0.513146	3.3:-89525703	None:intergenic
AGGGAAAGAACTGAGGGTAA+TGG	0.514591	3.3:-89525735	None:intergenic
AAGTTTGCCGATATTGGCTG+TGG	0.514722	3.3:+89525790	MS.gene40349:CDS
GAAAGAACTGAGGGTAATGG+AGG	0.517145	3.3:-89525732	None:intergenic
TAACAGATGTGGAAGAACTT+GGG	0.526686	3.3:+89526145	MS.gene40349:CDS
AAGAGAAGAACCATCGCCGT+CGG	0.534336	3.3:+89526055	MS.gene40349:CDS
CTATCAGCTTTCTCAGACGA+GGG	0.534942	3.3:-89525754	None:intergenic
CAATGCGTCTGTATACAGCC+TGG	0.550730	3.3:-89526311	None:intergenic
GGCTGATAACCCGACGGCGA+TGG	0.555787	3.3:-89526065	None:intergenic
TAAACAGACATGCCTGAGAC+TGG	0.565306	3.3:+89525580	None:intergenic
ACCAAAATGTTTCTGTGGTG+CGG	0.567556	3.3:+89525947	MS.gene40349:CDS
AATTCGTTCCTTAACATACT+CGG	0.570956	3.3:-89525893	None:intergenic
TTCCCAGAAACATTGATGAT+CGG	0.572410	3.3:+89525847	MS.gene40349:CDS
GAGCTTAAAAGCTTAACAGC+CGG	0.573038	3.3:-89526240	None:intergenic
ATGTGGAAGAACTTGGGGAC+TGG	0.574117	3.3:+89526151	MS.gene40349:CDS
TGACTGGGTGATAACGGCAC+AGG	0.576851	3.3:-89525697	None:intergenic
AGAAACATTGATGATCGGAA+TGG	0.582107	3.3:+89525852	MS.gene40349:CDS
AAAGAACTGAGGGTAATGGA+GGG	0.599329	3.3:-89525731	None:intergenic
TATATACACAATAACAGATG+TGG	0.600775	3.3:+89526134	MS.gene40349:CDS
AACAGATGTGGAAGAACTTG+GGG	0.605004	3.3:+89526146	MS.gene40349:CDS
GGAAATGAGAGTCACTGAGC+GGG	0.605858	3.3:-89525675	None:intergenic
GACTGGGTGATAACGGCACA+GGG	0.611699	3.3:-89525696	None:intergenic
TCAGTCAAAGGTTCAAACAA+CGG	0.617042	3.3:-89526198	None:intergenic
TCAATACCAAAATGTTTCTG+TGG	0.618646	3.3:+89525942	MS.gene40349:CDS
CCAAAAGGTTGCTAGAAACG+AGG	0.621682	3.3:+89526278	MS.gene40349:CDS
AGGGAATAGTCAACATGACT+GGG	0.631622	3.3:-89525712	None:intergenic
ACAGACATGCCTGAGACTGG+TGG	0.638512	3.3:+89525583	None:intergenic
GGGAAATGAGAGTCACTGAG+CGG	0.640601	3.3:-89525676	None:intergenic
CAAAAGGTTGCTAGAAACGA+GGG	0.641444	3.3:+89526279	MS.gene40349:CDS
ACTATCAGCTTTCTCAGACG+AGG	0.648094	3.3:-89525755	None:intergenic
CAATGTTTCTGGGAACAGAG+TGG	0.655902	3.3:-89525839	None:intergenic
CAGACGAGGGAAAGAACTGA+GGG	0.659441	3.3:-89525741	None:intergenic
TCAGACGAGGGAAAGAACTG+AGG	0.695327	3.3:-89525742	None:intergenic
AGAATGGGCTGATAACCCGA+CGG	0.724299	3.3:-89526071	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TATATACACAATAACAGATG+TGG	+	chr3.3:89526134-89526153	MS.gene40349:CDS	25.0%
!!	AGGAACGAATTTTATCTTTA+AGG	+	chr3.3:89525905-89525924	MS.gene40349:CDS	25.0%
!!	GGAACGAATTTTATCTTTAA+GGG	+	chr3.3:89525906-89525925	MS.gene40349:CDS	25.0%
	AAGATTAAGTTTGCCGATAT+TGG	+	chr3.3:89525784-89525803	MS.gene40349:CDS	30.0%
	AATTCGTTCCTTAACATACT+CGG	-	chr3.3:89525896-89525915	None:intergenic	30.0%
	CATACTCATCTAGTAAGAAT+GGG	-	chr3.3:89526089-89526108	None:intergenic	30.0%
!	TATGCTTTTGTTCTTGAGAT+TGG	+	chr3.3:89526105-89526124	MS.gene40349:CDS	30.0%
!	TCAATACCAAAATGTTTCTG+TGG	+	chr3.3:89525942-89525961	MS.gene40349:CDS	30.0%
!!	CTTCTCTTTAAAATGAGGAT+CGG	-	chr3.3:89526043-89526062	None:intergenic	30.0%
!!	TGGTTCTTCTCTTTAAAATG+AGG	-	chr3.3:89526048-89526067	None:intergenic	30.0%
!!	TTCTCTTTAAAATGAGGATC+GGG	-	chr3.3:89526042-89526061	None:intergenic	30.0%
	ATAACAGATGTGGAAGAACT+TGG	+	chr3.3:89526144-89526163	MS.gene40349:CDS	35.0%
	ATTCCGATCATCAATGTTTC+TGG	-	chr3.3:89525853-89525872	None:intergenic	35.0%
	GCATACTCATCTAGTAAGAA+TGG	-	chr3.3:89526090-89526109	None:intergenic	35.0%
	TAACAGATGTGGAAGAACTT+GGG	+	chr3.3:89526145-89526164	MS.gene40349:CDS	35.0%
	TCAACATTCAGTAAGTCAAC+AGG	+	chr3.3:89525610-89525629	MS.gene40349:CDS	35.0%
	TCAAGTTCTTTCTCAGTCAA+AGG	-	chr3.3:89526213-89526232	None:intergenic	35.0%
	TCAGTCAAAGGTTCAAACAA+CGG	-	chr3.3:89526201-89526220	None:intergenic	35.0%
	TGTGCTTTCTCAAAGTGATT+GGG	-	chr3.3:89525994-89526013	None:intergenic	35.0%
	TTCCGATCATCAATGTTTCT+GGG	-	chr3.3:89525852-89525871	None:intergenic	35.0%
	TTGTGCTTTCTCAAAGTGAT+TGG	-	chr3.3:89525995-89526014	None:intergenic	35.0%
!	AGAAACATTGATGATCGGAA+TGG	+	chr3.3:89525852-89525871	MS.gene40349:CDS	35.0%
!	TAAAGTGTCCGAGTATGTTA+AGG	+	chr3.3:89525885-89525904	MS.gene40349:CDS	35.0%
!	TTCCCAGAAACATTGATGAT+CGG	+	chr3.3:89525847-89525866	MS.gene40349:CDS	35.0%
!!!	GAAACATTTTGGTATTGACC+TGG	-	chr3.3:89525940-89525959	None:intergenic	35.0%
	AACAGATGTGGAAGAACTTG+GGG	+	chr3.3:89526146-89526165	MS.gene40349:CDS	40.0%
	AGGGAATAGTCAACATGACT+GGG	-	chr3.3:89525715-89525734	None:intergenic	40.0%
	CAAAAGGTTGCTAGAAACGA+GGG	+	chr3.3:89526279-89526298	MS.gene40349:CDS	40.0%
	CAGTCAAAGGTTCAAACAAC+GGG	-	chr3.3:89526200-89526219	None:intergenic	40.0%
	GAAAGAACTTGAAGCAGATC+CGG	+	chr3.3:89526221-89526240	MS.gene40349:CDS	40.0%
	GAGCTTAAAAGCTTAACAGC+CGG	-	chr3.3:89526243-89526262	None:intergenic	40.0%
	GGACTGGATGAAATCTTGTT+TGG	+	chr3.3:89526167-89526186	MS.gene40349:CDS	40.0%
	GTGATTGGGAATGTACTTCA+TGG	-	chr3.3:89525980-89525999	None:intergenic	40.0%
	TAAAATGAGGATCGGGAAAC+AGG	-	chr3.3:89526035-89526054	None:intergenic	40.0%
	TCAACATGACTGGGTGATAA+CGG	-	chr3.3:89525706-89525725	None:intergenic	40.0%
	TCCAAAACCACAGCCAATAT+CGG	-	chr3.3:89525800-89525819	None:intergenic	40.0%
!	ACCAAAATGTTTCTGTGGTG+CGG	+	chr3.3:89525947-89525966	MS.gene40349:CDS	40.0%
!	GCTTTTGTTCTTGAGATTGG+TGG	+	chr3.3:89526108-89526127	MS.gene40349:CDS	40.0%
!	TCTTTAAGGGTAGCAAATCC+AGG	+	chr3.3:89525919-89525938	MS.gene40349:CDS	40.0%
!!	AAAGAACTGAGGGTAATGGA+GGG	-	chr3.3:89525734-89525753	None:intergenic	40.0%
!!!	ATATTGGCTGTGGTTTTGGA+GGG	+	chr3.3:89525800-89525819	MS.gene40349:CDS	40.0%
	AAACGAGGGACAAACTTTCC+AGG	+	chr3.3:89526293-89526312	MS.gene40349:CDS	45.0%
	ACTATCAGCTTTCTCAGACG+AGG	-	chr3.3:89525758-89525777	None:intergenic	45.0%
	AGGGAAAGAACTGAGGGTAA+TGG	-	chr3.3:89525738-89525757	None:intergenic	45.0%
	CCAAAAGGTTGCTAGAAACG+AGG	+	chr3.3:89526278-89526297	MS.gene40349:CDS	45.0%
	CTATCAGCTTTCTCAGACGA+GGG	-	chr3.3:89525757-89525776	None:intergenic	45.0%
	GAGGGAATAGTCAACATGAC+TGG	-	chr3.3:89525716-89525735	None:intergenic	45.0%
	GTTGAATTTCCACCAGTCTC+AGG	-	chr3.3:89525595-89525614	None:intergenic	45.0%
	TTAAGCTCTGCCACTGAAGA+AGG	+	chr3.3:89526255-89526274	MS.gene40349:CDS	45.0%
!	AAGTTTGCCGATATTGGCTG+TGG	+	chr3.3:89525790-89525809	MS.gene40349:CDS	45.0%
!	GAAAGAACTGAGGGTAATGG+AGG	-	chr3.3:89525735-89525754	None:intergenic	45.0%
!	TCCGCACCACAGAAACATTT+TGG	-	chr3.3:89525951-89525970	None:intergenic	45.0%
!!	AACCTTTTGGCCTTCTTCAG+TGG	-	chr3.3:89526268-89526287	None:intergenic	45.0%
!!	CAATGTTTCTGGGAACAGAG+TGG	-	chr3.3:89525842-89525861	None:intergenic	45.0%
!!!	CCTCGTTTCTAGCAACCTTT+TGG	-	chr3.3:89526281-89526300	None:intergenic	45.0%
!!!	GATATTGGCTGTGGTTTTGG+AGG	+	chr3.3:89525799-89525818	MS.gene40349:CDS	45.0%
	AAGAGAAGAACCATCGCCGT+CGG	+	chr3.3:89526055-89526074	MS.gene40349:CDS	50.0%
	ATGTGGAAGAACTTGGGGAC+TGG	+	chr3.3:89526151-89526170	MS.gene40349:CDS	50.0%
	CAATGCGTCTGTATACAGCC+TGG	-	chr3.3:89526314-89526333	None:intergenic	50.0%
	CAGACGAGGGAAAGAACTGA+GGG	-	chr3.3:89525744-89525763	None:intergenic	50.0%
	GGAAATGAGAGTCACTGAGC+GGG	-	chr3.3:89525678-89525697	None:intergenic	50.0%
	GGGAAATGAGAGTCACTGAG+CGG	-	chr3.3:89525679-89525698	None:intergenic	50.0%
	TCAGACGAGGGAAAGAACTG+AGG	-	chr3.3:89525745-89525764	None:intergenic	50.0%
	TGCCACTGAAGAAGGCCAAA+AGG	+	chr3.3:89526263-89526282	MS.gene40349:CDS	50.0%
!!	AGAATGGGCTGATAACCCGA+CGG	-	chr3.3:89526074-89526093	None:intergenic	50.0%
!!	GCCGATATTGGCTGTGGTTT+TGG	+	chr3.3:89525796-89525815	MS.gene40349:CDS	50.0%
	AGAGAAGAACCATCGCCGTC+GGG	+	chr3.3:89526056-89526075	MS.gene40349:CDS	55.0%
!!	GACTGGGTGATAACGGCACA+GGG	-	chr3.3:89525699-89525718	None:intergenic	55.0%
!!	GCTCGATAGAAGCGCTTTCG+AGG	-	chr3.3:89525640-89525659	None:intergenic	55.0%
!!	TGACTGGGTGATAACGGCAC+AGG	-	chr3.3:89525700-89525719	None:intergenic	55.0%
	GGCTGATAACCCGACGGCGA+TGG	-	chr3.3:89526068-89526087	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	89525589	89526356	89525589	ID=MS.gene40349
chr3.3	mRNA	89525589	89526356	89525589	ID=MS.gene40349.t1;Parent=MS.gene40349
chr3.3	exon	89525589	89526356	89525589	ID=MS.gene40349.t1.exon1;Parent=MS.gene40349.t1
chr3.3	CDS	89525589	89526356	89525589	ID=cds.MS.gene40349.t1;Parent=MS.gene40349.t1

Gene Sequence

>MS.gene40349

ATGCCTGAGACTGGTGGAAATTCAACATTCAGTAAGTCAACAGGATTGCCTCGAAAGCGCTTCTATCGAGCACGAGCACACAGCAACCCGCTCAGTGACTCTCATTTCCCTGTGCCGTTATCACCCAGTCATGTTGACTATTCCCTCCATTACCCTCAGTTCTTTCCCTCGTCTGAGAAAGCTGATAGTTGCAAAAAGATTAAGTTTGCCGATATTGGCTGTGGTTTTGGAGGGTTGCTCATCAGTCTTTCCACTCTGTTCCCAGAAACATTGATGATCGGAATGGAACTTAGAGATAAAGTGTCCGAGTATGTTAAGGAACGAATTTTATCTTTAAGGGTAGCAAATCCAGGTCAATACCAAAATGTTTCTGTGGTGCGGACTAACTCCATGAAGTACATTCCCAATCACTTTGAGAAAGCACAACTCTCAAAGATGTTTTTCCTGTTTCCCGATCCTCATTTTAAAGAGAAGAACCATCGCCGTCGGGTTATCAGCCCATTCTTACTAGATGAGTATGCTTTTGTTCTTGAGATTGGTGGAATTATATACACAATAACAGATGTGGAAGAACTTGGGGACTGGATGAAATCTTGTTTGGAGAATCACCCGTTGTTTGAACCTTTGACTGAGAAAGAACTTGAAGCAGATCCGGCTGTTAAGCTTTTAAGCTCTGCCACTGAAGAAGGCCAAAAGGTTGCTAGAAACGAGGGACAAACTTTCCAGGCTGTATACAGACGCATTGCAGCATCTGATCAAATATCCTAG

Protein sequence

>MS.gene40349.t1

MPETGGNSTFSKSTGLPRKRFYRARAHSNPLSDSHFPVPLSPSHVDYSLHYPQFFPSSEKADSCKKIKFADIGCGFGGLLISLSTLFPETLMIGMELRDKVSEYVKERILSLRVANPGQYQNVSVVRTNSMKYIPNHFEKAQLSKMFFLFPDPHFKEKNHRRRVISPFLLDEYAFVLEIGGIIYTITDVEELGDWMKSCLENHPLFEPLTEKELEADPAVKLLSSATEEGQKVARNEGQTFQAVYRRIAASDQIS