AlfalfaGEDB | MS.gene055454

M. sativa cultivar XinJiangDaYe / MS.gene055454

KEGG: K03439
GO: GO:0003674 GO:0003824 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0008168 GO:0008173 GO:0008175 GO:0008176 GO:0008757 GO:0009451 GO:0009987 GO:0016070 GO:0016423 GO:0016740 GO:0016741 GO:0034470 GO:0034641 GO:0034660 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0046483 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360
PFAM: PF02390.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055454.t1	XP_003603585.1	99.2	255	2	0	1	255	1	255	7.60E-142	513.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055454.t1	Q8GXB7	79.8	243	46	2	8	249	8	248	2.5e-109	396.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055454.t1	G7J2W7	99.2	255	2	0	1	255	1	255	5.5e-142	513.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene055453	MS.gene055454	0.934254	5.37E-96	-1.69E-46
MS.gene055454	MS.gene055455	0.946698	2.81E-105	-1.69E-46
MS.gene055454	MS.gene066830	0.81508	1.11E-51	-1.69E-46
MS.gene055454	MS.gene40349	0.978609	3.47E-146	-1.69E-46
MS.gene055454	MS.gene75598	0.82273	2.03E-53	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene055454	MS.gene40390	PPI
MS.gene055454	MS.gene71227	PPI
MS.gene055454	MS.gene61042	PPI
MS.gene055454	MS.gene50496	PPI
MS.gene055454	MS.gene038492	PPI
MS.gene055454	MS.gene048631	PPI
MS.gene055454	MS.gene018116	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055454.t1	MTR_3g109360	99.216	255	2	0	1	255	1	255	0.0	528

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055454.t1	AT5G24840	79.835	243	46	2	8	249	8	248	1.12e-146	410

Find 65 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGATTAAGTTTGCCGATAT+TGG	0.221557	3.3:+89698178	MS.gene055454:CDS
TCCGCACCACAGAAACATTT+TGG	0.250656	3.3:-89698342	None:intergenic
ATTCCGATCATCAATGTTTC+TGG	0.252188	3.3:-89698244	None:intergenic
GCCGATATTGGCTGTGGTTT+TGG	0.274610	3.3:+89698190	MS.gene055454:CDS
CCTCGTTTCTAGCAACCTTT+TGG	0.326676	3.3:-89698672	None:intergenic
TAAAATGAGGATCGGGAAAC+AGG	0.368009	3.3:-89698426	None:intergenic
TTCTCTTTAAAATGAGGATC+GGG	0.398927	3.3:-89698433	None:intergenic
GCATACTCATCTAGTAAGAA+TGG	0.400409	3.3:-89698481	None:intergenic
AAACGAGGGACAAACTTTCC+AGG	0.404412	3.3:+89698687	MS.gene055454:CDS
TTGTGCTTTCTCAAAGTGAT+TGG	0.404689	3.3:-89698386	None:intergenic
GGACTGGATGAAATCTTGTT+TGG	0.409104	3.3:+89698561	MS.gene055454:CDS
TCCAAAACCACAGCCAATAT+CGG	0.417152	3.3:-89698191	None:intergenic
TTAAGCTCTGCCACTGAAGA+AGG	0.428496	3.3:+89698649	MS.gene055454:CDS
TGTGCTTTCTCAAAGTGATT+GGG	0.444654	3.3:-89698385	None:intergenic
TCAACATTCAGTAAGTCAAC+AGG	0.452366	3.3:+89698004	MS.gene055454:CDS
GTGATTGGGAATGTACTTCA+TGG	0.454613	3.3:-89698371	None:intergenic
GTTGAATTTCCACCAGTCTC+AGG	0.455044	3.3:-89697986	None:intergenic
TGGTTCTTCTCTTTAAAATG+AGG	0.455252	3.3:-89698439	None:intergenic
CAGTCAAAGGTTCAAACAAC+GGG	0.464877	3.3:-89698591	None:intergenic
TAAAGTGTCCGAGTATGTTA+AGG	0.471708	3.3:+89698279	MS.gene055454:CDS
TCAAGTTCTTTCTCAGTCAA+AGG	0.473439	3.3:-89698604	None:intergenic
ATAACAGATGTGGAAGAACT+TGG	0.476880	3.3:+89698538	MS.gene055454:CDS
GCTCGATAGAAGCGCTTTCG+AGG	0.477207	3.3:-89698031	None:intergenic
TCTTTAAGGGTAGCAAATCC+AGG	0.486768	3.3:+89698313	MS.gene055454:CDS
GAAAGAACTTGAAGCAGATC+CGG	0.487319	3.3:+89698615	MS.gene055454:CDS
TTCCGATCATCAATGTTTCT+GGG	0.488618	3.3:-89698243	None:intergenic
AGAGAAGAACCATCGCCGTC+GGG	0.492041	3.3:+89698450	MS.gene055454:CDS
GAGGGAATAGTCAACATGAC+TGG	0.493842	3.3:-89698107	None:intergenic
CTTCTCTTTAAAATGAGGAT+CGG	0.494124	3.3:-89698434	None:intergenic
CATACTCATCTAGTAAGAAT+GGG	0.503167	3.3:-89698480	None:intergenic
TGCCACTGAAGAAGGCCAAA+AGG	0.507812	3.3:+89698657	MS.gene055454:CDS
TCAACATGACTGGGTGATAA+CGG	0.513146	3.3:-89698097	None:intergenic
AGGGAAAGAACTGAGGGTAA+TGG	0.514591	3.3:-89698129	None:intergenic
AAGTTTGCCGATATTGGCTG+TGG	0.514722	3.3:+89698184	MS.gene055454:CDS
GAAAGAACTGAGGGTAATGG+AGG	0.517145	3.3:-89698126	None:intergenic
TAACAGATGTGGAAGAACTT+GGG	0.526686	3.3:+89698539	MS.gene055454:CDS
AAGAGAAGAACCATCGCCGT+CGG	0.534336	3.3:+89698449	MS.gene055454:CDS
CTATCAGCTTTCTCAGACGA+GGG	0.534942	3.3:-89698148	None:intergenic
CAATGCGTCTGTATACAGCC+TGG	0.550730	3.3:-89698705	None:intergenic
GGCTGATAACCCGACGGCGA+TGG	0.555787	3.3:-89698459	None:intergenic
TAAACAGACATGCCTGAGAC+TGG	0.565306	3.3:+89697974	None:intergenic
ACCAAAATGTTTCTGTGGTG+CGG	0.567556	3.3:+89698341	MS.gene055454:CDS
AATTCGTTCCTTAACATACT+CGG	0.570956	3.3:-89698287	None:intergenic
TTCCCAGAAACATTGATGAT+CGG	0.572410	3.3:+89698241	MS.gene055454:CDS
GAGCTTAAAAGCTTAACAGC+CGG	0.573038	3.3:-89698634	None:intergenic
ATGTGGAAGAACTTGGGGAC+TGG	0.574117	3.3:+89698545	MS.gene055454:CDS
TGACTGGGTGATAACGGCAC+AGG	0.576851	3.3:-89698091	None:intergenic
AGAAACATTGATGATCGGAA+TGG	0.582107	3.3:+89698246	MS.gene055454:CDS
AAAGAACTGAGGGTAATGGA+GGG	0.599329	3.3:-89698125	None:intergenic
TATATACACAATAACAGATG+TGG	0.600775	3.3:+89698528	MS.gene055454:CDS
AACAGATGTGGAAGAACTTG+GGG	0.605004	3.3:+89698540	MS.gene055454:CDS
GGAAATGAGAGTCACTGAGC+GGG	0.605858	3.3:-89698069	None:intergenic
GACTGGGTGATAACGGCACA+GGG	0.611699	3.3:-89698090	None:intergenic
TCAGTCAAAGGTTCAAACAA+CGG	0.617042	3.3:-89698592	None:intergenic
TCAATACCAAAATGTTTCTG+TGG	0.618646	3.3:+89698336	MS.gene055454:CDS
CCAAAAGGTTGCTAGAAACG+AGG	0.621682	3.3:+89698672	MS.gene055454:CDS
AGGGAATAGTCAACATGACT+GGG	0.631622	3.3:-89698106	None:intergenic
ACAGACATGCCTGAGACTGG+TGG	0.638512	3.3:+89697977	None:intergenic
GGGAAATGAGAGTCACTGAG+CGG	0.640601	3.3:-89698070	None:intergenic
CAAAAGGTTGCTAGAAACGA+GGG	0.641444	3.3:+89698673	MS.gene055454:CDS
ACTATCAGCTTTCTCAGACG+AGG	0.648094	3.3:-89698149	None:intergenic
CAATGTTTCTGGGAACAGAG+TGG	0.655902	3.3:-89698233	None:intergenic
CAGACGAGGGAAAGAACTGA+GGG	0.659441	3.3:-89698135	None:intergenic
TCAGACGAGGGAAAGAACTG+AGG	0.695327	3.3:-89698136	None:intergenic
AGAATGGGCTGATAACCCGA+CGG	0.724299	3.3:-89698465	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TATATACACAATAACAGATG+TGG	+	chr3.3:89698528-89698547	MS.gene055454:CDS	25.0%
!!	AGGAACGAATTTTATCTTTA+AGG	+	chr3.3:89698299-89698318	MS.gene055454:CDS	25.0%
!!	GGAACGAATTTTATCTTTAA+GGG	+	chr3.3:89698300-89698319	MS.gene055454:CDS	25.0%
	AAGATTAAGTTTGCCGATAT+TGG	+	chr3.3:89698178-89698197	MS.gene055454:CDS	30.0%
	AATTCGTTCCTTAACATACT+CGG	-	chr3.3:89698290-89698309	None:intergenic	30.0%
	CATACTCATCTAGTAAGAAT+GGG	-	chr3.3:89698483-89698502	None:intergenic	30.0%
!	TATGCTTTTGTTCTTGAGAT+TGG	+	chr3.3:89698499-89698518	MS.gene055454:CDS	30.0%
!	TCAATACCAAAATGTTTCTG+TGG	+	chr3.3:89698336-89698355	MS.gene055454:CDS	30.0%
!!	CTTCTCTTTAAAATGAGGAT+CGG	-	chr3.3:89698437-89698456	None:intergenic	30.0%
!!	TGGTTCTTCTCTTTAAAATG+AGG	-	chr3.3:89698442-89698461	None:intergenic	30.0%
!!	TTCTCTTTAAAATGAGGATC+GGG	-	chr3.3:89698436-89698455	None:intergenic	30.0%
	ATAACAGATGTGGAAGAACT+TGG	+	chr3.3:89698538-89698557	MS.gene055454:CDS	35.0%
	ATTCCGATCATCAATGTTTC+TGG	-	chr3.3:89698247-89698266	None:intergenic	35.0%
	GCATACTCATCTAGTAAGAA+TGG	-	chr3.3:89698484-89698503	None:intergenic	35.0%
	TAACAGATGTGGAAGAACTT+GGG	+	chr3.3:89698539-89698558	MS.gene055454:CDS	35.0%
	TCAACATTCAGTAAGTCAAC+AGG	+	chr3.3:89698004-89698023	MS.gene055454:CDS	35.0%
	TCAAGTTCTTTCTCAGTCAA+AGG	-	chr3.3:89698607-89698626	None:intergenic	35.0%
	TCAGTCAAAGGTTCAAACAA+CGG	-	chr3.3:89698595-89698614	None:intergenic	35.0%
	TGTGCTTTCTCAAAGTGATT+GGG	-	chr3.3:89698388-89698407	None:intergenic	35.0%
	TTCCGATCATCAATGTTTCT+GGG	-	chr3.3:89698246-89698265	None:intergenic	35.0%
	TTGTGCTTTCTCAAAGTGAT+TGG	-	chr3.3:89698389-89698408	None:intergenic	35.0%
!	AGAAACATTGATGATCGGAA+TGG	+	chr3.3:89698246-89698265	MS.gene055454:CDS	35.0%
!	TAAAGTGTCCGAGTATGTTA+AGG	+	chr3.3:89698279-89698298	MS.gene055454:CDS	35.0%
!	TTCCCAGAAACATTGATGAT+CGG	+	chr3.3:89698241-89698260	MS.gene055454:CDS	35.0%
!!!	GAAACATTTTGGTATTGACC+TGG	-	chr3.3:89698334-89698353	None:intergenic	35.0%
	AACAGATGTGGAAGAACTTG+GGG	+	chr3.3:89698540-89698559	MS.gene055454:CDS	40.0%
	AGGGAATAGTCAACATGACT+GGG	-	chr3.3:89698109-89698128	None:intergenic	40.0%
	CAAAAGGTTGCTAGAAACGA+GGG	+	chr3.3:89698673-89698692	MS.gene055454:CDS	40.0%
	CAGTCAAAGGTTCAAACAAC+GGG	-	chr3.3:89698594-89698613	None:intergenic	40.0%
	GAAAGAACTTGAAGCAGATC+CGG	+	chr3.3:89698615-89698634	MS.gene055454:CDS	40.0%
	GAGCTTAAAAGCTTAACAGC+CGG	-	chr3.3:89698637-89698656	None:intergenic	40.0%
	GGACTGGATGAAATCTTGTT+TGG	+	chr3.3:89698561-89698580	MS.gene055454:CDS	40.0%
	GTGATTGGGAATGTACTTCA+TGG	-	chr3.3:89698374-89698393	None:intergenic	40.0%
	TAAAATGAGGATCGGGAAAC+AGG	-	chr3.3:89698429-89698448	None:intergenic	40.0%
	TCAACATGACTGGGTGATAA+CGG	-	chr3.3:89698100-89698119	None:intergenic	40.0%
	TCCAAAACCACAGCCAATAT+CGG	-	chr3.3:89698194-89698213	None:intergenic	40.0%
!	ACCAAAATGTTTCTGTGGTG+CGG	+	chr3.3:89698341-89698360	MS.gene055454:CDS	40.0%
!	GCTTTTGTTCTTGAGATTGG+TGG	+	chr3.3:89698502-89698521	MS.gene055454:CDS	40.0%
!	TCTTTAAGGGTAGCAAATCC+AGG	+	chr3.3:89698313-89698332	MS.gene055454:CDS	40.0%
!!	AAAGAACTGAGGGTAATGGA+GGG	-	chr3.3:89698128-89698147	None:intergenic	40.0%
!!!	ATATTGGCTGTGGTTTTGGA+GGG	+	chr3.3:89698194-89698213	MS.gene055454:CDS	40.0%
	AAACGAGGGACAAACTTTCC+AGG	+	chr3.3:89698687-89698706	MS.gene055454:CDS	45.0%
	ACTATCAGCTTTCTCAGACG+AGG	-	chr3.3:89698152-89698171	None:intergenic	45.0%
	AGGGAAAGAACTGAGGGTAA+TGG	-	chr3.3:89698132-89698151	None:intergenic	45.0%
	CCAAAAGGTTGCTAGAAACG+AGG	+	chr3.3:89698672-89698691	MS.gene055454:CDS	45.0%
	CTATCAGCTTTCTCAGACGA+GGG	-	chr3.3:89698151-89698170	None:intergenic	45.0%
	GAGGGAATAGTCAACATGAC+TGG	-	chr3.3:89698110-89698129	None:intergenic	45.0%
	GTTGAATTTCCACCAGTCTC+AGG	-	chr3.3:89697989-89698008	None:intergenic	45.0%
	TTAAGCTCTGCCACTGAAGA+AGG	+	chr3.3:89698649-89698668	MS.gene055454:CDS	45.0%
!	AAGTTTGCCGATATTGGCTG+TGG	+	chr3.3:89698184-89698203	MS.gene055454:CDS	45.0%
!	GAAAGAACTGAGGGTAATGG+AGG	-	chr3.3:89698129-89698148	None:intergenic	45.0%
!	TCCGCACCACAGAAACATTT+TGG	-	chr3.3:89698345-89698364	None:intergenic	45.0%
!!	AACCTTTTGGCCTTCTTCAG+TGG	-	chr3.3:89698662-89698681	None:intergenic	45.0%
!!	CAATGTTTCTGGGAACAGAG+TGG	-	chr3.3:89698236-89698255	None:intergenic	45.0%
!!!	CCTCGTTTCTAGCAACCTTT+TGG	-	chr3.3:89698675-89698694	None:intergenic	45.0%
!!!	GATATTGGCTGTGGTTTTGG+AGG	+	chr3.3:89698193-89698212	MS.gene055454:CDS	45.0%
	AAGAGAAGAACCATCGCCGT+CGG	+	chr3.3:89698449-89698468	MS.gene055454:CDS	50.0%
	ATGTGGAAGAACTTGGGGAC+TGG	+	chr3.3:89698545-89698564	MS.gene055454:CDS	50.0%
	CAATGCGTCTGTATACAGCC+TGG	-	chr3.3:89698708-89698727	None:intergenic	50.0%
	CAGACGAGGGAAAGAACTGA+GGG	-	chr3.3:89698138-89698157	None:intergenic	50.0%
	GGAAATGAGAGTCACTGAGC+GGG	-	chr3.3:89698072-89698091	None:intergenic	50.0%
	GGGAAATGAGAGTCACTGAG+CGG	-	chr3.3:89698073-89698092	None:intergenic	50.0%
	TCAGACGAGGGAAAGAACTG+AGG	-	chr3.3:89698139-89698158	None:intergenic	50.0%
	TGCCACTGAAGAAGGCCAAA+AGG	+	chr3.3:89698657-89698676	MS.gene055454:CDS	50.0%
!!	AGAATGGGCTGATAACCCGA+CGG	-	chr3.3:89698468-89698487	None:intergenic	50.0%
!!	GCCGATATTGGCTGTGGTTT+TGG	+	chr3.3:89698190-89698209	MS.gene055454:CDS	50.0%
	AGAGAAGAACCATCGCCGTC+GGG	+	chr3.3:89698450-89698469	MS.gene055454:CDS	55.0%
!!	GACTGGGTGATAACGGCACA+GGG	-	chr3.3:89698093-89698112	None:intergenic	55.0%
!!	GCTCGATAGAAGCGCTTTCG+AGG	-	chr3.3:89698034-89698053	None:intergenic	55.0%
!!	TGACTGGGTGATAACGGCAC+AGG	-	chr3.3:89698094-89698113	None:intergenic	55.0%
	GGCTGATAACCCGACGGCGA+TGG	-	chr3.3:89698462-89698481	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	89697983	89698750	89697983	ID=MS.gene055454
chr3.3	mRNA	89697983	89698750	89697983	ID=MS.gene055454.t1;Parent=MS.gene055454
chr3.3	exon	89697983	89698750	89697983	ID=MS.gene055454.t1.exon1;Parent=MS.gene055454.t1
chr3.3	CDS	89697983	89698750	89697983	ID=cds.MS.gene055454.t1;Parent=MS.gene055454.t1

Gene Sequence

>MS.gene055454

ATGCCTGAGACTGGTGGAAATTCAACATTCAGTAAGTCAACAGGATTGCCTCGAAAGCGCTTCTATCGAGCACGAGCACACAGCAACCCGCTCAGTGACTCTCATTTCCCTGTGCCGTTATCACCCAGTCATGTTGACTATTCCCTCCATTACCCTCAGTTCTTTCCCTCGTCTGAGAAAGCTGATAGTTGCAAAAAGATTAAGTTTGCCGATATTGGCTGTGGTTTTGGAGGGTTGCTCATCAGTCTTTCCACTCTGTTCCCAGAAACATTGATGATCGGAATGGAACTTAGAGATAAAGTGTCCGAGTATGTTAAGGAACGAATTTTATCTTTAAGGGTAGCAAATCCAGGTCAATACCAAAATGTTTCTGTGGTGCGGACTAACTCCATGAAGTACATTCCCAATCACTTTGAGAAAGCACAACTCTCAAAGATGTTTTTCCTGTTTCCCGATCCTCATTTTAAAGAGAAGAACCATCGCCGTCGGGTTATCAGCCCATTCTTACTAGATGAGTATGCTTTTGTTCTTGAGATTGGTGGAATTATATACACAATAACAGATGTGGAAGAACTTGGGGACTGGATGAAATCTTGTTTGGAGAATCACCCGTTGTTTGAACCTTTGACTGAGAAAGAACTTGAAGCAGATCCGGCTGTTAAGCTTTTAAGCTCTGCCACTGAAGAAGGCCAAAAGGTTGCTAGAAACGAGGGACAAACTTTCCAGGCTGTATACAGACGCATTGCAGCATCTGATCAAATATCCTAG

Protein sequence

>MS.gene055454.t1

MPETGGNSTFSKSTGLPRKRFYRARAHSNPLSDSHFPVPLSPSHVDYSLHYPQFFPSSEKADSCKKIKFADIGCGFGGLLISLSTLFPETLMIGMELRDKVSEYVKERILSLRVANPGQYQNVSVVRTNSMKYIPNHFEKAQLSKMFFLFPDPHFKEKNHRRRVISPFLLDEYAFVLEIGGIIYTITDVEELGDWMKSCLENHPLFEPLTEKELEADPAVKLLSSATEEGQKVARNEGQTFQAVYRRIAASDQIS