AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene039281

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene039281.t1 MTR_2g072470 98.812 421 3 1 1 419 1 421 0.0 851
MS.gene039281.t1 MTR_4g095055 36.292 383 222 6 39 399 9 391 2.00e-76 243
MS.gene039281.t1 MTR_4g095055 35.192 287 164 6 39 303 9 295 7.81e-46 165
MS.gene039281.t1 MTR_4g095055 39.583 96 58 0 304 399 369 464 6.59e-17 82.8
MS.gene039281.t1 MTR_8g078070 37.349 249 136 7 111 346 92 333 4.26e-35 133
MS.gene039281.t1 MTR_5g019460 37.008 254 142 6 111 352 91 338 1.32e-34 132
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene039281.t1 AT1G17050 73.352 364 97 0 55 418 53 416 0.0 548
MS.gene039281.t1 AT1G78510 67.064 419 122 3 2 418 1 405 0.0 545
MS.gene039281.t1 AT1G78510 78.743 334 71 0 85 418 33 366 0.0 540
MS.gene039281.t1 AT2G34630 39.809 314 176 3 101 401 85 398 1.69e-72 234
MS.gene039281.t1 AT2G34630 40.067 297 165 3 118 401 1 297 4.26e-69 221
MS.gene039281.t1 AT4G36810 41.176 255 126 9 109 346 85 332 4.67e-38 141
MS.gene039281.t1 AT2G18620 40.152 264 137 9 111 360 66 322 5.03e-36 135
MS.gene039281.t1 AT2G18640 38.000 250 137 7 109 346 90 333 2.43e-34 131
MS.gene039281.t1 AT3G14510 39.331 239 125 6 136 360 27 259 2.94e-33 126
MS.gene039281.t1 AT3G20160 35.827 254 140 8 109 346 59 305 1.63e-31 123
MS.gene039281.t1 AT3G32040 35.955 267 150 7 109 360 75 335 3.65e-29 117
MS.gene039281.t1 AT2G23800 35.857 251 141 9 109 346 94 337 2.00e-28 115
MS.gene039281.t1 AT3G29430 35.955 267 150 7 109 360 72 332 1.10e-27 113
MS.gene039281.t1 AT3G14550 35.955 267 150 8 109 360 75 335 1.83e-27 112
MS.gene039281.t1 AT3G14550 35.955 267 150 8 109 360 75 335 1.83e-27 112
MS.gene039281.t1 AT3G14550 35.955 267 150 8 109 360 75 335 1.83e-27 112
MS.gene039281.t1 AT3G14530 36.330 267 149 8 109 360 75 335 5.55e-27 111
MS.gene039281.t1 AT3G20160 33.603 247 127 8 109 346 59 277 1.24e-26 109
MS.gene039281.t1 AT1G49530 32.444 225 130 6 137 345 78 296 1.84e-22 97.8

Find 71 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAACCTATTCGGTGAAAT+AGG 0.205067 2.2:-20265123 None:intergenic
TCAATATCGTGTTTCTTCTA+TGG 0.220495 2.2:+20263975 MS.gene039281:CDS
GAGACCAGCTCTTGTGTTCT+TGG 0.226567 2.2:+20265729 MS.gene039281:CDS
TTGATTGCTGCCAGTGTAAA+AGG 0.265491 2.2:+20267009 MS.gene039281:CDS
GGCTAAACGTCGATGCTTTA+CGG 0.286782 2.2:-20265929 None:intergenic
ACAACCTATTCGGTGAAATA+GGG 0.302959 2.2:-20265122 None:intergenic
TCACTAGCTTACCTCTTCTT+AGG 0.317476 2.2:-20266014 None:intergenic
TGGAAATGGAGCTCGTAAAT+TGG 0.324226 2.2:+20263927 MS.gene039281:CDS
GAAACTGTTCATCAAATGTT+TGG 0.338601 2.2:+20266478 MS.gene039281:CDS
CCAAGTTGTCGACGACATAT+TGG 0.347498 2.2:+20267110 MS.gene039281:CDS
TCAATGATATCCCTCAATTT+CGG 0.393056 2.2:-20267231 None:intergenic
TTCGGTGAAATAGGGGTTGC+TGG 0.399015 2.2:-20265114 None:intergenic
TGGAGCGGTCAGGTTACCTT+TGG 0.414371 2.2:-20267184 None:intergenic
CCAATATGTCGTCGACAACT+TGG 0.427937 2.2:-20267110 None:intergenic
AATTCTAAAGGGTGTGGAAA+TGG 0.428535 2.2:+20263913 MS.gene039281:CDS
GAGTCTGAATTTAGCGAGAT+TGG 0.432083 2.2:+20267252 MS.gene039281:CDS
CAACCCCTATTTCACCGAAT+AGG 0.446689 2.2:+20265118 MS.gene039281:CDS
GCAATTAAAGACCTTGCAAA+AGG 0.452757 2.2:+20265072 MS.gene039281:CDS
TCAGCTGCAGATATTAAAAC+TGG 0.453325 2.2:-20265676 None:intergenic
TATGCTAAGGTGAATTCTAA+AGG 0.455161 2.2:+20263901 MS.gene039281:CDS
TCAAGTTGGTCAAGAGTTGC+GGG 0.455657 2.2:+20267292 MS.gene039281:CDS
ACTACTTCAAACAACCTATT+CGG 0.464935 2.2:-20265132 None:intergenic
ACCGAGAAAATGTTCGAATA+CGG 0.464949 2.2:+20267066 MS.gene039281:CDS
TTGGCGAGGTCCGTGCCAGC+AGG 0.477676 2.2:-20267165 None:intergenic
TGGAGAAAGAACCGAAATTG+AGG 0.478658 2.2:+20267220 MS.gene039281:CDS
GAGCAACTTGGAAAGCCTGC+TGG 0.484511 2.2:+20267150 MS.gene039281:CDS
ACTTGGAAAGCCTGCTGGCA+CGG 0.485083 2.2:+20267155 MS.gene039281:CDS
ACAAGAGTGGCAGTGCTGGC+TGG 0.494391 2.2:+20266502 MS.gene039281:CDS
ACGCAATCGGCAGAGCAACT+TGG 0.497280 2.2:+20267138 MS.gene039281:CDS
TTCGAATACGGGAAGAATCT+CGG 0.498347 2.2:+20267078 MS.gene039281:CDS
ATGAATCATTTCAACTATCT+CGG 0.499817 2.2:-20265950 None:intergenic
ATGCTAAGGTGAATTCTAAA+GGG 0.500417 2.2:+20263902 MS.gene039281:CDS
TGGAACAAGAGTGGCAGTGC+TGG 0.503897 2.2:+20266498 MS.gene039281:CDS
ATTGAGTGTTTGAAGATCAT+CGG 0.505570 2.2:-20265157 None:intergenic
GGAGAAAGAACCGAAATTGA+GGG 0.507533 2.2:+20267221 MS.gene039281:CDS
AATTGGTTTGTTGTAAGAAG+AGG 0.509786 2.2:+20263944 MS.gene039281:CDS
CATATTGGATTTCACGCAAT+CGG 0.510526 2.2:+20267125 MS.gene039281:CDS
TCTTTAGTGCTGGTGGGAAG+AGG 0.511599 2.2:+20265704 MS.gene039281:CDS
ACTGCAGAGCCTACTTTGAA+TGG 0.516676 2.2:+20264000 MS.gene039281:CDS
TCATGTTTGCACAATCATCA+TGG 0.521316 2.2:+20266530 MS.gene039281:CDS
ATGCCTATGAAAAGAAGCAT+TGG 0.522548 2.2:-20263866 None:intergenic
GGATGCTCTTGATAGATTGT+AGG 0.526067 2.2:+20265643 MS.gene039281:intron
CCGAGAAAATGTTCGAATAC+GGG 0.527115 2.2:+20267067 MS.gene039281:CDS
ATCAAGTTGGTCAAGAGTTG+CGG 0.532769 2.2:+20267291 MS.gene039281:CDS
AGTCATTAAACTTATCAGCC+AGG 0.539916 2.2:+20266579 MS.gene039281:CDS
AAGGTGAATTCTAAAGGGTG+TGG 0.545230 2.2:+20263907 MS.gene039281:CDS
GACACCAAGAACACAAGAGC+TGG 0.548114 2.2:-20265733 None:intergenic
CAACCTATTCGGTGAAATAG+GGG 0.554963 2.2:-20265121 None:intergenic
GCTGAGCAGATCTTTAGTGC+TGG 0.564731 2.2:+20265694 MS.gene039281:CDS
GAGCAGATCTTTAGTGCTGG+TGG 0.569958 2.2:+20265697 MS.gene039281:CDS
TCGTTTAGCTCTTGAGGACA+TGG 0.571941 2.2:+20267398 MS.gene039281:CDS
AGCAGATCTTTAGTGCTGGT+GGG 0.574274 2.2:+20265698 MS.gene039281:CDS
GATGAGAGTGACCTAAGAAG+AGG 0.576816 2.2:+20266003 MS.gene039281:CDS
AATCGAACGAGCACAAGAGT+TGG 0.579042 2.2:+20267317 MS.gene039281:CDS
TGGTGACATATAATCTTCAA+AGG 0.582035 2.2:+20267418 MS.gene039281:CDS
GCTGGCACGGACCTCGCCAA+AGG 0.585708 2.2:+20267168 MS.gene039281:CDS
TGAAGTTAAGAATTATGCTA+AGG 0.586221 2.2:+20263888 MS.gene039281:CDS
CGGTCAGGTTACCTTTGGCG+AGG 0.592711 2.2:-20267179 None:intergenic
TGTGTATCGTTTAGCTCTTG+AGG 0.595996 2.2:+20267392 MS.gene039281:CDS
TATGTCGTCGACAACTTGGA+AGG 0.598026 2.2:-20267106 None:intergenic
TCAAATGTTTGGAACAAGAG+TGG 0.606800 2.2:+20266489 MS.gene039281:CDS
AGAGCGACCGCAGAACTACA+TGG 0.608016 2.2:+20265757 MS.gene039281:CDS
ATCACTGTACCATTCAAAGT+AGG 0.612430 2.2:-20264009 None:intergenic
TGCTGCCAGTGTAAAAGGAG+CGG 0.624409 2.2:+20267014 MS.gene039281:CDS
TCTTGACGAAGCGATCAAGT+TGG 0.627382 2.2:+20267278 MS.gene039281:CDS
CTCAAGGCCATGTAGTTCTG+CGG 0.632644 2.2:-20265764 None:intergenic
CAAGTTGGTCAAGAGTTGCG+GGG 0.670668 2.2:+20267293 MS.gene039281:CDS
AGAGCTAAACGATACACACT+CGG 0.671348 2.2:-20267387 None:intergenic
AAGTTGGTCAAGAGTTGCGG+GGG 0.682829 2.2:+20267294 MS.gene039281:CDS
TACACTGGCAGCAATCAAGG+AGG 0.700199 2.2:-20267004 None:intergenic
GCTAAACGATACACACTCGG+AGG 0.746443 2.2:-20267384 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATGAAAAAATCATTATTT+CGG + chr2.2:20266792-20266811 MS.gene039281:intron 10.0%
!!! TTTTTATTTTGCAAAATTTT+TGG + chr2.2:20266868-20266887 MS.gene039281:intron 10.0%
!!! TTTTTCTTTCAAATTATTAT+CGG + chr2.2:20264910-20264929 MS.gene039281:intron 10.0%
!! TAAAAACAATGTTTAGAAAA+TGG - chr2.2:20266854-20266873 None:intergenic 15.0%
!! TGATCAAATAAATTATAACA+TGG - chr2.2:20266166-20266185 None:intergenic 15.0%
!!! TAACTTGTTTTGAATTAATA+GGG + chr2.2:20266452-20266471 MS.gene039281:intron 15.0%
!! AAGTAATTGATATCCATTTA+AGG - chr2.2:20266729-20266748 None:intergenic 20.0%
!! AATATGAAAGAACATATGTT+AGG + chr2.2:20266692-20266711 MS.gene039281:intron 20.0%
!! AGTAATTGATATCCATTTAA+GGG - chr2.2:20266728-20266747 None:intergenic 20.0%
!! ATTAAAATTGTCAGATATTG+TGG + chr2.2:20264499-20264518 MS.gene039281:intron 20.0%
!! CATCAGTTAAATTAACATAA+AGG - chr2.2:20265622-20265641 None:intergenic 20.0%
!! TGGTATATACATTCATAAAA+AGG + chr2.2:20266330-20266349 MS.gene039281:intron 20.0%
!! TTATGTTAATTTAACTGATG+AGG + chr2.2:20265622-20265641 MS.gene039281:intron 20.0%
!!! AGATATTAAAACTGGATTTT+CGG - chr2.2:20265671-20265690 None:intergenic 20.0%
!!! AGTATGACAAAAATTGATTT+AGG - chr2.2:20266117-20266136 None:intergenic 20.0%
!!! CTAACTTGTTTTGAATTAAT+AGG + chr2.2:20266451-20266470 MS.gene039281:intron 20.0%
!!! GTATGACAAAAATTGATTTA+GGG - chr2.2:20266116-20266135 None:intergenic 20.0%
!!! TCAATTTTTGTCATACTTTA+CGG + chr2.2:20266120-20266139 MS.gene039281:intron 20.0%
!!! TCTGACAATTTTAATATTGT+CGG - chr2.2:20264494-20264513 None:intergenic 20.0%
!!! TGATTTTTTTGGTAAAAACT+TGG + chr2.2:20263825-20263844 MS.gene039281:CDS 20.0%
! AACTTTGGCATTAAAAGATT+TGG + chr2.2:20265028-20265047 MS.gene039281:intron 25.0%
! AAGCTAGTGATATTTAGAAA+TGG + chr2.2:20266027-20266046 MS.gene039281:intron 25.0%
! AATGGATATCAATTACTTCA+AGG + chr2.2:20266731-20266750 MS.gene039281:intron 25.0%
! ATATGTTCTTTCATATTCAC+CGG - chr2.2:20266689-20266708 None:intergenic 25.0%
! ATCTTGAAATGAATGATTTC+AGG + chr2.2:20265899-20265918 MS.gene039281:intron 25.0%
! ATGAATCATTTCAACTATCT+CGG - chr2.2:20265953-20265972 None:intergenic 25.0%
! ATGGATATCAATTACTTCAA+GGG + chr2.2:20266732-20266751 MS.gene039281:intron 25.0%
! ATGTATATACCAATTTGTCA+AGG - chr2.2:20266322-20266341 None:intergenic 25.0%
! CCAAATTAAAACAAAGTTTC+AGG - chr2.2:20266218-20266237 None:intergenic 25.0%
! GTAATTGATATCCATTTAAG+GGG - chr2.2:20266727-20266746 None:intergenic 25.0%
! TAGATGGAAAAGAAAAAACA+AGG - chr2.2:20266387-20266406 None:intergenic 25.0%
! TCCTGAAAATTCTATGTATT+TGG + chr2.2:20265855-20265874 MS.gene039281:intron 25.0%
! TCTGACAATTCTAATATTGT+CGG - chr2.2:20264610-20264629 None:intergenic 25.0%
! TCTTGAAATGAATGATTTCA+GGG + chr2.2:20265900-20265919 MS.gene039281:intron 25.0%
! TGAAGTTAAGAATTATGCTA+AGG + chr2.2:20263888-20263907 MS.gene039281:CDS 25.0%
! TGTGATATACAATTTAGTAC+AGG - chr2.2:20265455-20265474 None:intergenic 25.0%
!! ATTGTTCAGTGATTTTAAAC+AGG + chr2.2:20265337-20265356 MS.gene039281:intron 25.0%
!! GTTTAAAATCACTGAACAAT+GGG - chr2.2:20265337-20265356 None:intergenic 25.0%
!! TAATAATTTGAAGTGTGTTG+TGG + chr2.2:20264049-20264068 MS.gene039281:intron 25.0%
!! TAATGACTTCAATGTTTTCA+AGG - chr2.2:20266567-20266586 None:intergenic 25.0%
!! TGTTTAAAATCACTGAACAA+TGG - chr2.2:20265338-20265357 None:intergenic 25.0%
!! TTCATTTTGTATGTACTATG+TGG + chr2.2:20264209-20264228 MS.gene039281:intron 25.0%
!!! AGCAAAAGCATTGATTTTTT+TGG + chr2.2:20263814-20263833 MS.gene039281:CDS 25.0%
!!! ATTTGGTTTGGTTTTTGAAT+AGG + chr2.2:20265045-20265064 MS.gene039281:intron 25.0%
!!! CCTGAAACTTTGTTTTAATT+TGG + chr2.2:20266215-20266234 MS.gene039281:intron 25.0%
!!! TTGTTTTGAATTAATAGGGA+AGG + chr2.2:20266456-20266475 MS.gene039281:intron 25.0%
AAATTGTCAGATATTGTGGA+AGG + chr2.2:20264503-20264522 MS.gene039281:intron 30.0%
ACAATATCTGTCAATTCTGT+TGG - chr2.2:20264844-20264863 None:intergenic 30.0%
ACTACTTCAAACAACCTATT+CGG - chr2.2:20265135-20265154 None:intergenic 30.0%
AGAAAAGCTGAAAAGTAAGA+AGG - chr2.2:20265565-20265584 None:intergenic 30.0%
ATCAATTACTTCAAGGGATT+GGG + chr2.2:20266738-20266757 MS.gene039281:intron 30.0%
ATGCTAAGGTGAATTCTAAA+GGG + chr2.2:20263902-20263921 MS.gene039281:CDS 30.0%
ATTGAGTGTTTGAAGATCAT+CGG - chr2.2:20265160-20265179 None:intergenic 30.0%
CTTTCAAATTATTATCGGTC+TGG + chr2.2:20264915-20264934 MS.gene039281:intron 30.0%
GAAACTGTTCATCAAATGTT+TGG + chr2.2:20266478-20266497 MS.gene039281:CDS 30.0%
GAAAGAACATATGTTAGGAT+AGG + chr2.2:20266697-20266716 MS.gene039281:intron 30.0%
GACACAATTATAAGCAACTA+AGG - chr2.2:20265247-20265266 None:intergenic 30.0%
GAGATTTGTATACTTACTCA+AGG - chr2.2:20265783-20265802 None:intergenic 30.0%
GATTGAATGTTACATATGTC+TGG - chr2.2:20264304-20264323 None:intergenic 30.0%
GTATCAGAATTATCTGATAC+CGG + chr2.2:20264155-20264174 MS.gene039281:intron 30.0%
TATCAATTACTTCAAGGGAT+TGG + chr2.2:20266737-20266756 MS.gene039281:intron 30.0%
TATGCTAAGGTGAATTCTAA+AGG + chr2.2:20263901-20263920 MS.gene039281:CDS 30.0%
TATGTATCACCTTGACAAAT+TGG + chr2.2:20266310-20266329 MS.gene039281:intron 30.0%
TATGTTCTTTCATATTCACC+GGG - chr2.2:20266688-20266707 None:intergenic 30.0%
TCAATATCGTGTTTCTTCTA+TGG + chr2.2:20263975-20263994 MS.gene039281:CDS 30.0%
TCAATGATATCCCTCAATTT+CGG - chr2.2:20267234-20267253 None:intergenic 30.0%
TGGTGACATATAATCTTCAA+AGG + chr2.2:20267418-20267437 MS.gene039281:CDS 30.0%
TTCTCCAATGCAAAAATTAC+TGG - chr2.2:20267207-20267226 None:intergenic 30.0%
TTGTATGAGTCATTACATGT+CGG + chr2.2:20266257-20266276 MS.gene039281:intron 30.0%
TTTCAAATTCTGCTATGCTA+TGG + chr2.2:20265490-20265509 MS.gene039281:intron 30.0%
TTTCTGCTTATGATCATGTT+TGG + chr2.2:20266079-20266098 MS.gene039281:intron 30.0%
! ACAAAAATTGATTTAGGGCA+TGG - chr2.2:20266111-20266130 None:intergenic 30.0%
! GCCAAATACATAGAATTTTC+AGG - chr2.2:20265859-20265878 None:intergenic 30.0%
! GTGATTAAAGATTTTGCTAG+TGG + chr2.2:20266901-20266920 MS.gene039281:CDS 30.0%
! TATCTTATTGAAAGTGTGTC+TGG + chr2.2:20264123-20264142 MS.gene039281:intron 30.0%
! TGGCATTAAAAGATTTGGTT+TGG + chr2.2:20265033-20265052 MS.gene039281:intron 30.0%
! TTCATTTTGTACGTACTATG+TGG + chr2.2:20264667-20264686 MS.gene039281:intron 30.0%
!! AATTGGTTTGTTGTAAGAAG+AGG + chr2.2:20263944-20263963 MS.gene039281:CDS 30.0%
!! TGATTTTAGAAGTGGAACTT+TGG + chr2.2:20265013-20265032 MS.gene039281:intron 30.0%
!!! GTTCGTATTGATTTTAGAAG+TGG + chr2.2:20265005-20265024 MS.gene039281:intron 30.0%
!!! TGTAGTACTGATACTTTTGA+TGG + chr2.2:20264227-20264246 MS.gene039281:intron 30.0%
AATACTCAAACAGACAAGAC+AGG - chr2.2:20265269-20265288 None:intergenic 35.0%
ACCGAGAAAATGTTCGAATA+CGG + chr2.2:20267066-20267085 MS.gene039281:CDS 35.0%
AGTCATTAAACTTATCAGCC+AGG + chr2.2:20266579-20266598 MS.gene039281:CDS 35.0%
ATCACTGTACCATTCAAAGT+AGG - chr2.2:20264012-20264031 None:intergenic 35.0%
CAATGCAAAAATTACTGGAG+CGG - chr2.2:20267202-20267221 None:intergenic 35.0%
CATATTGGATTTCACGCAAT+CGG + chr2.2:20267125-20267144 MS.gene039281:CDS 35.0%
CTTTCAACGTTGTATGATGA+TGG - chr2.2:20266611-20266630 None:intergenic 35.0%
GAATTGTCAGATATTGTCGA+AGG + chr2.2:20264619-20264638 MS.gene039281:intron 35.0%
GACTGAATGAAACATGTGTA+TGG - chr2.2:20264187-20264206 None:intergenic 35.0%
GATATTGTTGAACACCAAAC+AGG + chr2.2:20264856-20264875 MS.gene039281:intron 35.0%
GCAATTAAAGACCTTGCAAA+AGG + chr2.2:20265072-20265091 MS.gene039281:CDS 35.0%
GGTAAAAACTTGGTTTCTTG+TGG + chr2.2:20263835-20263854 MS.gene039281:CDS 35.0%
TCAGCTGCAGATATTAAAAC+TGG - chr2.2:20265679-20265698 None:intergenic 35.0%
TCATGTTTGCACAATCATCA+TGG + chr2.2:20266530-20266549 MS.gene039281:CDS 35.0%
TGTGGTACTAATACTTCTGA+TGG + chr2.2:20264685-20264704 MS.gene039281:intron 35.0%
! AACAACCTATTCGGTGAAAT+AGG - chr2.2:20265126-20265145 None:intergenic 35.0%
! AATTCTAAAGGGTGTGGAAA+TGG + chr2.2:20263913-20263932 MS.gene039281:CDS 35.0%
! ACAACCTATTCGGTGAAATA+GGG - chr2.2:20265125-20265144 None:intergenic 35.0%
! AGTGTAGCGGAATTCAAAAA+TGG - chr2.2:20265393-20265412 None:intergenic 35.0%
! ATGCCTATGAAAAGAAGCAT+TGG - chr2.2:20263869-20263888 None:intergenic 35.0%
! CTATGTACTGATACTTCTGA+TGG + chr2.2:20264452-20264471 MS.gene039281:intron 35.0%
! CTTCCAATGCTTCTTTTCAT+AGG + chr2.2:20263863-20263882 MS.gene039281:CDS 35.0%
! CTTCTGATCAAAAGTGTGTA+TGG + chr2.2:20264357-20264376 MS.gene039281:intron 35.0%
! TCAAATGTTTGGAACAAGAG+TGG + chr2.2:20266489-20266508 MS.gene039281:CDS 35.0%
! TCAAGTTCAATCCTTTTGCA+AGG - chr2.2:20265086-20265105 None:intergenic 35.0%
! TTCATTTTGTACGTGCTATG+TGG + chr2.2:20264551-20264570 MS.gene039281:intron 35.0%
!! GACTTCAATGTTTTCAAGGT+TGG - chr2.2:20266563-20266582 None:intergenic 35.0%
!!! CAAAATTTTTGGTGTGTGAC+AGG + chr2.2:20266879-20266898 MS.gene039281:intron 35.0%
!!! TGGTTTGGTTTTTGAATAGG+TGG + chr2.2:20265048-20265067 MS.gene039281:intron 35.0%
ACCACATCGAGTTTGAATAG+AGG - chr2.2:20266429-20266448 None:intergenic 40.0%
ACTATAGCGCTATAGTGTAG+CGG - chr2.2:20265406-20265425 None:intergenic 40.0%
AGAGCTAAACGATACACACT+CGG - chr2.2:20267390-20267409 None:intergenic 40.0%
CCGAGAAAATGTTCGAATAC+GGG + chr2.2:20267067-20267086 MS.gene039281:CDS 40.0%
CGAGTTTGAATAGAGGCAAA+TGG - chr2.2:20266422-20266441 None:intergenic 40.0%
GAACTGTCAGATATTGTCGA+AGG + chr2.2:20264278-20264297 MS.gene039281:intron 40.0%
GAGTCTGAATTTAGCGAGAT+TGG + chr2.2:20267252-20267271 MS.gene039281:CDS 40.0%
GCAGATGCAAGTGAATGATA+AGG - chr2.2:20266637-20266656 None:intergenic 40.0%
GGAGAAAGAACCGAAATTGA+GGG + chr2.2:20267221-20267240 MS.gene039281:CDS 40.0%
GGATGCTCTTGATAGATTGT+AGG + chr2.2:20265643-20265662 MS.gene039281:intron 40.0%
GTTACATGTGTTTGCCTGTT+TGG - chr2.2:20264873-20264892 None:intergenic 40.0%
TAGCTGCTATTGGACAATAC+TGG + chr2.2:20265518-20265537 MS.gene039281:intron 40.0%
TCCTTTACACCTCACACATA+TGG + chr2.2:20266192-20266211 MS.gene039281:intron 40.0%
TGGAAATGGAGCTCGTAAAT+TGG + chr2.2:20263927-20263946 MS.gene039281:CDS 40.0%
TGGAGAAAGAACCGAAATTG+AGG + chr2.2:20267220-20267239 MS.gene039281:CDS 40.0%
TTCGAATACGGGAAGAATCT+CGG + chr2.2:20267078-20267097 MS.gene039281:CDS 40.0%
TTGATTGCTGCCAGTGTAAA+AGG + chr2.2:20267009-20267028 MS.gene039281:CDS 40.0%
TTGTCAAATAGCCGCTATTG+CGG - chr2.2:20265430-20265449 None:intergenic 40.0%
! AAGGTGAATTCTAAAGGGTG+TGG + chr2.2:20263907-20263926 MS.gene039281:CDS 40.0%
! CAACCTATTCGGTGAAATAG+GGG - chr2.2:20265124-20265143 None:intergenic 40.0%
! CCCGTATTCGAACATTTTCT+CGG - chr2.2:20267070-20267089 None:intergenic 40.0%
! CGCTCCAGTAATTTTTGCAT+TGG + chr2.2:20267200-20267219 MS.gene039281:CDS 40.0%
! GGGTATCATTCACGTTTTTC+TGG - chr2.2:20265317-20265336 None:intergenic 40.0%
! TCACTAGCTTACCTCTTCTT+AGG - chr2.2:20266017-20266036 None:intergenic 40.0%
! TTCTTTTCCATCTACTCTGC+TGG + chr2.2:20266393-20266412 MS.gene039281:intron 40.0%
! TTTTACACTGGCAGCAATCA+AGG - chr2.2:20267010-20267029 None:intergenic 40.0%
!! ATCAAGTTGGTCAAGAGTTG+CGG + chr2.2:20267291-20267310 MS.gene039281:CDS 40.0%
!! CTTTTGATGGAAAGTGTGTC+TGG + chr2.2:20264240-20264259 MS.gene039281:intron 40.0%
!! TGTGGTACTGATACTTCTGA+TGG + chr2.2:20264569-20264588 MS.gene039281:intron 40.0%
!! TGTGTATCGTTTAGCTCTTG+AGG + chr2.2:20267392-20267411 MS.gene039281:CDS 40.0%
AAAGTGTGTCTGGTGTCTGA+CGG + chr2.2:20264250-20264269 MS.gene039281:intron 45.0%
AACATGTGTATGGAGTTCGC+CGG - chr2.2:20264177-20264196 None:intergenic 45.0%
AATCGAACGAGCACAAGAGT+TGG + chr2.2:20267317-20267336 MS.gene039281:CDS 45.0%
ACTGCAGAGCCTACTTTGAA+TGG + chr2.2:20264000-20264019 MS.gene039281:CDS 45.0%
AGTTTCAGGCCATATGTGTG+AGG - chr2.2:20266204-20266223 None:intergenic 45.0%
CAAAAATTACTGGAGCGGTC+AGG - chr2.2:20267197-20267216 None:intergenic 45.0%
CAACCCCTATTTCACCGAAT+AGG + chr2.2:20265118-20265137 MS.gene039281:CDS 45.0%
CCAAGTTGTCGACGACATAT+TGG + chr2.2:20267110-20267129 MS.gene039281:CDS 45.0%
CCAATATGTCGTCGACAACT+TGG - chr2.2:20267113-20267132 None:intergenic 45.0%
GAAAAGTAAGAAGGCTCAGC+AGG - chr2.2:20265556-20265575 None:intergenic 45.0%
GATGAGAGTGACCTAAGAAG+AGG + chr2.2:20266003-20266022 MS.gene039281:CDS 45.0%
GCCATATGTGTGAGGTGTAA+AGG - chr2.2:20266196-20266215 None:intergenic 45.0%
GCCTCTATTCAAACTCGATG+TGG + chr2.2:20266425-20266444 MS.gene039281:intron 45.0%
GGATAGGTCAACCCCTTAAA+TGG + chr2.2:20266713-20266732 MS.gene039281:intron 45.0%
GGCTAAACGTCGATGCTTTA+CGG - chr2.2:20265932-20265951 None:intergenic 45.0%
TATGGCGCTATAGCTGCTAT+TGG + chr2.2:20265508-20265527 MS.gene039281:intron 45.0%
TATGTCGTCGACAACTTGGA+AGG - chr2.2:20267109-20267128 None:intergenic 45.0%
TCTTGACGAAGCGATCAAGT+TGG + chr2.2:20267278-20267297 MS.gene039281:CDS 45.0%
TCTTTCATATTCACCGGGCA+GGG - chr2.2:20266683-20266702 None:intergenic 45.0%
TTCTTTCATATTCACCGGGC+AGG - chr2.2:20266684-20266703 None:intergenic 45.0%
! ACTTGCGTGCAGGGTTATTT+TGG + chr2.2:20266767-20266786 MS.gene039281:intron 45.0%
! CTTCTGATGGAAAGTGTGTC+TGG + chr2.2:20264465-20264484 MS.gene039281:intron 45.0%
! GACATGTAAGTGTGTCACTG+CGG + chr2.2:20264937-20264956 MS.gene039281:intron 45.0%
!! AGCAGATCTTTAGTGCTGGT+GGG + chr2.2:20265698-20265717 MS.gene039281:CDS 45.0%
!! GTATGATGATGGAACTCACC+TGG - chr2.2:20266600-20266619 None:intergenic 45.0%
!! TCAAGTTGGTCAAGAGTTGC+GGG + chr2.2:20267292-20267311 MS.gene039281:CDS 45.0%
!! TCGTTTAGCTCTTGAGGACA+TGG + chr2.2:20267398-20267417 MS.gene039281:CDS 45.0%
AATAACCCTGCACGCAAGTG+TGG - chr2.2:20266766-20266785 None:intergenic 50.0%
ATGGAGACCAGCAGAGTAGA+TGG - chr2.2:20266403-20266422 None:intergenic 50.0%
GACACCAAGAACACAAGAGC+TGG - chr2.2:20265736-20265755 None:intergenic 50.0%
GAGACCAGCTCTTGTGTTCT+TGG + chr2.2:20265729-20265748 MS.gene039281:CDS 50.0%
GCGCTATAGTACCGCAATAG+CGG + chr2.2:20265416-20265435 MS.gene039281:intron 50.0%
GCTAAACGATACACACTCGG+AGG - chr2.2:20267387-20267406 None:intergenic 50.0%
GCTGAGCAGATCTTTAGTGC+TGG + chr2.2:20265694-20265713 MS.gene039281:CDS 50.0%
TACACTGGCAGCAATCAAGG+AGG - chr2.2:20267007-20267026 None:intergenic 50.0%
TGCTGCCAGTGTAAAAGGAG+CGG + chr2.2:20267014-20267033 MS.gene039281:CDS 50.0%
TTCCAAGATTCAACCCTGCC+CGG + chr2.2:20266667-20266686 MS.gene039281:intron 50.0%
TTCGGTGAAATAGGGGTTGC+TGG - chr2.2:20265117-20265136 None:intergenic 50.0%
! AAAGGAGCGGCAGTTTTCAG+CGG + chr2.2:20267027-20267046 MS.gene039281:CDS 50.0%
! AACTGCCGCTCCTTTTACAC+TGG - chr2.2:20267022-20267041 None:intergenic 50.0%
! AAGTTGGTCAAGAGTTGCGG+GGG + chr2.2:20267294-20267313 MS.gene039281:CDS 50.0%
! CTCAAGGCCATGTAGTTCTG+CGG - chr2.2:20265767-20265786 None:intergenic 50.0%
! GAGCAGATCTTTAGTGCTGG+TGG + chr2.2:20265697-20265716 MS.gene039281:CDS 50.0%
!! CAAGTTGGTCAAGAGTTGCG+GGG + chr2.2:20267293-20267312 MS.gene039281:CDS 50.0%
!! TCTTTAGTGCTGGTGGGAAG+AGG + chr2.2:20265704-20265723 MS.gene039281:CDS 50.0%
ACGCAATCGGCAGAGCAACT+TGG + chr2.2:20267138-20267157 MS.gene039281:CDS 55.0%
ACTTGGAAAGCCTGCTGGCA+CGG + chr2.2:20267155-20267174 MS.gene039281:CDS 55.0%
AGAGCGACCGCAGAACTACA+TGG + chr2.2:20265757-20265776 MS.gene039281:CDS 55.0%
GAGCAACTTGGAAAGCCTGC+TGG + chr2.2:20267150-20267169 MS.gene039281:CDS 55.0%
TGGAACAAGAGTGGCAGTGC+TGG + chr2.2:20266498-20266517 MS.gene039281:CDS 55.0%
TGGAGCGGTCAGGTTACCTT+TGG - chr2.2:20267187-20267206 None:intergenic 55.0%
CACCGGGCAGGGTTGAATCT+TGG - chr2.2:20266672-20266691 None:intergenic 60.0%
CGGTCAGGTTACCTTTGGCG+AGG - chr2.2:20267182-20267201 None:intergenic 60.0%
!! ACAAGAGTGGCAGTGCTGGC+TGG + chr2.2:20266502-20266521 MS.gene039281:CDS 60.0%
GGGCTCCACACTTGCGTGCA+GGG + chr2.2:20266758-20266777 MS.gene039281:intron 65.0%
TGGGCTCCACACTTGCGTGC+AGG + chr2.2:20266757-20266776 MS.gene039281:intron 65.0%
GCTGGCACGGACCTCGCCAA+AGG + chr2.2:20267168-20267187 MS.gene039281:CDS 70.0%
TTGGCGAGGTCCGTGCCAGC+AGG - chr2.2:20267168-20267187 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 20263799 20267449 20263799 ID=MS.gene039281
chr2.2 mRNA 20263799 20267449 20263799 ID=MS.gene039281.t1;Parent=MS.gene039281
chr2.2 exon 20263799 20264021 20263799 ID=MS.gene039281.t1.exon1;Parent=MS.gene039281.t1
chr2.2 CDS 20263799 20264021 20263799 ID=cds.MS.gene039281.t1;Parent=MS.gene039281.t1
chr2.2 exon 20265067 20265194 20265067 ID=MS.gene039281.t1.exon2;Parent=MS.gene039281.t1
chr2.2 CDS 20265067 20265194 20265067 ID=cds.MS.gene039281.t1;Parent=MS.gene039281.t1
chr2.2 exon 20265658 20265785 20265658 ID=MS.gene039281.t1.exon3;Parent=MS.gene039281.t1
chr2.2 CDS 20265658 20265785 20265658 ID=cds.MS.gene039281.t1;Parent=MS.gene039281.t1
chr2.2 exon 20265921 20266024 20265921 ID=MS.gene039281.t1.exon4;Parent=MS.gene039281.t1
chr2.2 CDS 20265921 20266024 20265921 ID=cds.MS.gene039281.t1;Parent=MS.gene039281.t1
chr2.2 exon 20266473 20266600 20266473 ID=MS.gene039281.t1.exon5;Parent=MS.gene039281.t1
chr2.2 CDS 20266473 20266600 20266473 ID=cds.MS.gene039281.t1;Parent=MS.gene039281.t1
chr2.2 exon 20266901 20267449 20266901 ID=MS.gene039281.t1.exon6;Parent=MS.gene039281.t1
chr2.2 CDS 20266901 20267449 20266901 ID=cds.MS.gene039281.t1;Parent=MS.gene039281.t1
Gene Sequence

>MS.gene039281

ATGATGTCTGTCACTAGCAAAAGCATTGATTTTTTTGGTAAAAACTTGGTTTCTTGTGGCTGTTCTTCCAATGCTTCTTTTCATAGGCATGAAGTTAAGAATTATGCTAAGGTGAATTCTAAAGGGTGTGGAAATGGAGCTCGTAAATTGGTTTGTTGTAAGAAGAGGAATACTCATCAATATCGTGTTTCTTCTATGGATACTGCAGAGCCTACTTTGAATGGTACAGTGATATTTTATTTTATTTTTTTAATAATTTGAAGTGTGTTGTGGAGTTACTTCATGTTACTAGTTTGCATGAATGATTTTGTCTGTACTACGTAGTATCTTATTGAAAGTGTGTCTGGTGTTCGTCAGTATCAGAATTATCTGATACCGGCGAACTCCATACACATGTTTCATTCAGTCATTTCATTTTGTATGTACTATGTAGTACTGATACTTTTGATGGAAAGTGTGTCTGGTGTCTGACGGTATCAGAACTGTCAGATATTGTCGAAGGCTAGACATATGTAACATTCAATCATTTCATTTTGTATGTACTATGTAGCACTGATACTTCTGATCAAAAGTGTGTATGGTGTCTGACAGTATCAGAATTGTTCTGTCAAACGTCAACACATGTAACATTCAATCATTTCATTTTGTATGTACTATGTACTGATACTTCTGATGGAAAGTGTGTCTGGTTTCCGACAATATTAAAATTGTCAGATATTGTGGAAGGCCAGACATATGTAACATTCAATCATTTCATTTTGTACGTGCTATGTGGTACTGATACTTCTGATGGAAAGTGTGTCTGGTGCCGACAATATTAGAATTGTCAGATATTGTCGAAGGCCAGACATATGTAACATTCAATCATTTCATTTTGTACGTACTATGTGGTACTAATACTTCTGATGGAAAGTGTGTCTGGTGTCCGACAATATTAGAATTGTCAGATATTGTCGAACGTCAGACATATGTAACATTCAATCATTTCATTTTGTATGTACTATGTGGTACTGATACTTCTGATGGAAAGTGTGTCTGGTGTCCAACAGAATTGACAGATATTGTTGAACACCAAACAGGCAAACACATGTAACATTCAATTATTTCATTTTTTTTCTTTCAAATTATTATCGGTCTGGACATGTAAGTGTGTCACTGCGGTGTGAGTATTTGTGTCAGTGTTTTATAGCGAATGAAGTAATATATGTTCGTATTGATTTTAGAAGTGGAACTTTGGCATTAAAAGATTTGGTTTGGTTTTTGAATAGGTGGAGCAATTAAAGACCTTGCAAAAGGATTGAACTTGAAGAGTTATCCAGCAACCCCTATTTCACCGAATAGGTTGTTTGAAGTAGTCTCCGATGATCTTCAAACACTCAATAAAAATCTTCAGTCTGTAAGTATTGCTTTCTATATTCATTTTGTTTGTTGTTTTTTACTATCTGCCTTAGTTGCTTATAATTGTGTCCTGTCTTGTCTGTTTGAGTATTTCTTTGTTCTATTGACTTCAACAGACCAGAAAAACGTGAATGATACCCATTGTTCAGTGATTTTAAACAGGACATTCTTTGCTTATTGTTCTTTTAATAATCCATTTTTGAATTCCGCTACACTATAGCGCTATAGTACCGCAATAGCGGCTATTTGACAACACCTGTACTAAATTGTATATCACAAAACAATATTGATTTTTTCAAATTCTGCTATGCTATGGCGCTATAGCTGCTATTGGACAATACTGGTCATTCTTATTGCCTGCTGAGCCTTCTTACTTTTCAGCTTTTCTTACACTGCAAACAGTAATGATTAGATTTTTGTTGCCTTTATGTTAATTTAACTGATGAGGATGCTCTTGATAGATTGTAGGTGCCGAAAATCCAGTTTTAATATCTGCAGCTGAGCAGATCTTTAGTGCTGGTGGGAAGAGGATGAGACCAGCTCTTGTGTTCTTGGTGTCAAGAGCGACCGCAGAACTACATGGCCTTGAGTAAGTATACAAATCTCTCTTTATCTCTCTCACATGCGCGCGCACACGCAAAGAGTAGTCATTTGCTTGTCCTGAAAATTCTATGTATTTGGCTATAAAAAATTGAAAGCTTCATCTTGAAATGAATGATTTCAGGGATCTTACCGTAAAGCATCGACGTTTAGCCGAGATAGTTGAAATGATTCATACTGCAAGTTTGATACATGATGATGTGTTAGATGAGAGTGACCTAAGAAGAGGTAAGCTAGTGATATTTAGAAATGGCTGTTTAATGTTCTTGACATGACTTTATTTTTCTGCTTATGATCATGTTTGGCAACGTCCATGCCCTAAATCAATTTTTGTCATACTTTACGGTCGTTTTGATTTTAATCTTACCATGTTATAATTTATTTGATCAAGAATTTCCTTTACACCTCACACATATGGCCTGAAACTTTGTTTTAATTTGGAATTAAACATTATTGCTCGTTGTATGAGTCATTACATGTCGGTGTTAAGTACAAGTCTAAAGATACATCTGCTATGTATCACCTTGACAAATTGGTATATACATTCATAAAAAGGCTTCAAGTTTTCAAACTGTGACATTTGATTTCCTTGTTTTTTCTTTTCCATCTACTCTGCTGGTCTCCATTTGCCTCTATTCAAACTCGATGTGGTTGCTAACTTGTTTTGAATTAATAGGGAAGGAAACTGTTCATCAAATGTTTGGAACAAGAGTGGCAGTGCTGGCTGGAGACTTCATGTTTGCACAATCATCATGGTATCTCGCCAACCTTGAAAACATTGAAGTCATTAAACTTATCAGCCAGGTGAGTTCCATCATCATACAACGTTGAAAGTATCCTTATCATTCACTTGCATCTGCAAAATCATCATTCCAAGATTCAACCCTGCCCGGTGAATATGAAAGAACATATGTTAGGATAGGTCAACCCCTTAAATGGATATCAATTACTTCAAGGGATTGGGCTCCACACTTGCGTGCAGGGTTATTTTGGTCTAATGAAAAAATCATTATTTCGGAAGTGCTTAGCATAGTGTATGAAAAGATGCATTAGTCCATTTTCTAAACATTGTTTTTATTTTGCAAAATTTTTGGTGTGTGACAGGTGATTAAAGATTTTGCTAGTGGAGAAATAAAGCAAGCTTCTAGCTTGTTTGATTGTGATGTTCAGCTCGACGAGTATCTAATCAAAAGTTATTTTAAGACAGCCTCCTTGATTGCTGCCAGTGTAAAAGGAGCGGCAGTTTTCAGCGGTTCTGACAGCAGCATGACCGAGAAAATGTTCGAATACGGGAAGAATCTCGGTCTCTCCTTCCAAGTTGTCGACGACATATTGGATTTCACGCAATCGGCAGAGCAACTTGGAAAGCCTGCTGGCACGGACCTCGCCAAAGGTAACCTGACCGCTCCAGTAATTTTTGCATTGGAGAAAGAACCGAAATTGAGGGATATCATTGAGTCTGAATTTAGCGAGATTGGTTCTCTTGACGAAGCGATCAAGTTGGTCAAGAGTTGCGGGGGAATCGAACGAGCACAAGAGTTGGCAAAAGAGAAAGCTGATCTTGCTATTCAAAGTCTTCAATGTCTTCCTCCGAGTGTGTATCGTTTAGCTCTTGAGGACATGGTGACATATAATCTTCAAAGGATTGCATAA
Protein sequence

>MS.gene039281.t1

MMSVTSKSIDFFGKNLVSCGCSSNASFHRHEVKNYAKVNSKGCGNGARKLVCCKKRNTHQYRVSSMDTAEPTLNGGAIKDLAKGLNLKSYPATPISPNRLFEVVSDDLQTLNKNLQSIVGAENPVLISAAEQIFSAGGKRMRPALVFLVSRATAELHGLEDLTVKHRRLAEIVEMIHTASLIHDDVLDESDLRRGKETVHQMFGTRVAVLAGDFMFAQSSWYLANLENIEVIKLISQVIKDFASGEIKQASSLFDCDVQLDEYLIKSYFKTASLIAASVKGAAVFSGSDSSMTEKMFEYGKNLGLSFQVVDDILDFTQSAEQLGKPAGTDLAKGNLTAPVIFALEKEPKLRDIIESEFSEIGSLDEAIKLVKSCGGIERAQELAKEKADLAIQSLQCLPPSVYRLALEDMVTYNLQRIA