AlfalfaGEDB | MS.gene042957

M. sativa cultivar XinJiangDaYe / MS.gene042957

KEGG: K03627
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF01381.25 PF08523.13

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene042957.t1	AES76734.2	97.3	146	4	0	1	146	4	149	2.60E-70	274.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene042957.t1	Q9LV58	71.6	148	40	1	1	146	1	148	8.2e-49	194.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene042957.t1	G7KNH9	97.3	146	4	0	1	146	4	149	1.9e-70	274.6

TFs/TRs:

Gene ID	Type	Classification
MS.gene042957.t1	TR	MBF1

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene94585	MS.gene042957	PPI
MS.gene042957	MS.gene073154	PPI
MS.gene042957	MS.gene35285	PPI
MS.gene91241	MS.gene042957	PPI
MS.gene042957	MS.gene73865	PPI
MS.gene045483	MS.gene042957	PPI
MS.gene006697	MS.gene042957	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene042957.t1	MTR_6g086280	97.260	146	4	0	1	146	4	149	1.78e-100	285
MS.gene042957.t1	MTR_6g086280	97.222	144	4	0	1	144	4	147	4.12e-99	281
MS.gene042957.t1	MTR_2g084220	50.000	138	69	0	6	143	2	139	4.64e-43	139
MS.gene042957.t1	MTR_4g080090	50.725	138	68	0	6	143	4	141	9.39e-42	136
MS.gene042957.t1	MTR_6g018330	42.105	133	61	1	11	143	26	142	1.70e-26	97.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene042957.t1	AT3G24500	71.622	148	40	1	1	146	1	148	9.93e-69	204
MS.gene042957.t1	AT3G58680	52.174	138	66	0	6	143	4	141	4.19e-44	142
MS.gene042957.t1	AT2G42680	50.725	138	68	0	6	143	4	141	7.40e-43	139
MS.gene042957.t1	AT3G24500	58.947	95	37	1	1	93	1	95	8.16e-33	112

Find 44 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTTCGGAGGGCTTGGTTTA+TGG	0.142766	6.4:+59821880	None:intergenic
TTACGAAGGTCTTGTGCTTT+AGG	0.233932	6.4:+59821911	None:intergenic
GGGGAAAATGGAGAAGGTTT+TGG	0.237217	6.4:-59821591	MS.gene042957:CDS
TGTTATTAATCAAGGTGTTT+TGG	0.256794	6.4:-59821612	MS.gene042957:CDS
GTTAAACTTAGGGGTAAAAT+TGG	0.277746	6.4:-59821566	MS.gene042957:CDS
CTGCTCCGGTTCGGAGGGCT+TGG	0.299889	6.4:+59821873	None:intergenic
AGGGCTTGGTTTATGGCCTT+TGG	0.306456	6.4:+59821887	None:intergenic
TGAAAGGGTTCAGGTTGTTC+AGG	0.318932	6.4:-59821654	MS.gene042957:CDS
CGCGATACAGAAAGCGCGTT+TGG	0.332684	6.4:-59821717	MS.gene042957:CDS
ATTGATTAATGAAAGGGTTC+AGG	0.341931	6.4:-59821663	MS.gene042957:CDS
GAAGATGAGTCAGGGTGATT+TGG	0.359012	6.4:-59821690	MS.gene042957:CDS
CTGAACCGGCGGCGTTGGAG+CGG	0.395441	6.4:-59821760	MS.gene042957:CDS
GGGCTTGGTTTATGGCCTTT+GGG	0.397588	6.4:+59821888	None:intergenic
AATTTCCTCGCGTTTAAAAC+CGG	0.402472	6.4:+59821794	None:intergenic
GGCGAAATTGATTAATGAAA+GGG	0.407053	6.4:-59821669	MS.gene042957:CDS
ATTAGATGAAGCGGCTGAAC+CGG	0.407540	6.4:-59821774	MS.gene042957:CDS
AAGGTCTTGTGCTTTAGGTT+TGG	0.420759	6.4:+59821916	None:intergenic
TGAACCGGCGGCGTTGGAGC+GGG	0.428434	6.4:-59821759	MS.gene042957:CDS
GCGGCGTTGGAGCGGGTTGG+TGG	0.440938	6.4:-59821752	MS.gene042957:CDS
TAGGAACAATAAAACAAGAC+TGG	0.459948	6.4:-59821961	MS.gene042957:CDS
AGCGGCTGAACCGGCGGCGT+TGG	0.461093	6.4:-59821765	MS.gene042957:CDS
TTTAAAACCGGTCCGGAAGT+TGG	0.466419	6.4:+59821806	None:intergenic
ATAAACCAAGCCCTCCGAAC+CGG	0.471149	6.4:-59821878	MS.gene042957:CDS
TGGCGAAATTGATTAATGAA+AGG	0.476549	6.4:-59821670	MS.gene042957:CDS
TTTGGATAAGAAGATGAGTC+AGG	0.478923	6.4:-59821699	MS.gene042957:CDS
GGTAAAGCTGTTATTAATCA+AGG	0.485331	6.4:-59821620	MS.gene042957:CDS
TTAAGACCTCTGCTCCGGTT+CGG	0.505245	6.4:+59821864	None:intergenic
GACTGTTAAGACCTCTGCTC+CGG	0.505477	6.4:+59821859	None:intergenic
GACCTCTGCTCCGGTTCGGA+GGG	0.511130	6.4:+59821868	None:intergenic
AGACCTCTGCTCCGGTTCGG+AGG	0.519624	6.4:+59821867	None:intergenic
CCGGCGGCGTTGGAGCGGGT+TGG	0.528980	6.4:-59821755	MS.gene042957:CDS
CGCGAGGAAATTAGATGAAG+CGG	0.544491	6.4:-59821783	MS.gene042957:CDS
CCTCGCGTTTAAAACCGGTC+CGG	0.559526	6.4:+59821799	None:intergenic
GTTGTTCAGGAGTATGAGAA+TGG	0.565499	6.4:-59821641	MS.gene042957:CDS
AGCCCTCCGAACCGGAGCAG+AGG	0.582656	6.4:-59821870	MS.gene042957:CDS
CCAACCCGCTCCAACGCCGC+CGG	0.589439	6.4:+59821755	None:intergenic
ATTGTTCCTACGGTTCGTGT+CGG	0.592550	6.4:+59821974	None:intergenic
TTATGGCCTTTGGGTTACGA+AGG	0.594530	6.4:+59821897	None:intergenic
AGGAACAATAAAACAAGACT+GGG	0.609753	6.4:-59821960	MS.gene042957:CDS
AGATGAAGCGGCTGAACCGG+CGG	0.621947	6.4:-59821771	MS.gene042957:CDS
AAAACAAGACTGGGAACCAG+TGG	0.626445	6.4:-59821951	MS.gene042957:CDS
ACGATGCCGACACGAACCGT+AGG	0.633339	6.4:-59821980	MS.gene042957:CDS
TTGGATAAGAAGATGAGTCA+GGG	0.654305	6.4:-59821698	MS.gene042957:CDS
ACAAGACCTTCGTAACCCAA+AGG	0.679598	6.4:-59821903	MS.gene042957:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	GTTATTAATCAAGGTGTTTT+GGG	-	chr6.4:59821925-59821944	MS.gene042957:CDS	25.0%
!!!	TGTTATTAATCAAGGTGTTT+TGG	-	chr6.4:59821924-59821943	MS.gene042957:CDS	25.0%
!!!	TTATTAATCAAGGTGTTTTG+GGG	-	chr6.4:59821926-59821945	MS.gene042957:CDS	25.0%
	AGGAACAATAAAACAAGACT+GGG	-	chr6.4:59821576-59821595	MS.gene042957:CDS	30.0%
	ATTGATTAATGAAAGGGTTC+AGG	-	chr6.4:59821873-59821892	MS.gene042957:CDS	30.0%
	GGCGAAATTGATTAATGAAA+GGG	-	chr6.4:59821867-59821886	MS.gene042957:CDS	30.0%
	GGTAAAGCTGTTATTAATCA+AGG	-	chr6.4:59821916-59821935	MS.gene042957:CDS	30.0%
	GTTAAACTTAGGGGTAAAAT+TGG	-	chr6.4:59821970-59821989	MS.gene042957:CDS	30.0%
	TAGGAACAATAAAACAAGAC+TGG	-	chr6.4:59821575-59821594	MS.gene042957:CDS	30.0%
	TGGCGAAATTGATTAATGAA+AGG	-	chr6.4:59821866-59821885	MS.gene042957:CDS	30.0%
!!!	GTCTTGTTTTATTGTTCCTA+CGG	+	chr6.4:59821575-59821594	None:intergenic	30.0%
	TCGAACAAAAAACCAACTTC+CGG	-	chr6.4:59821718-59821737	MS.gene042957:CDS	35.0%
	TTGGATAAGAAGATGAGTCA+GGG	-	chr6.4:59821838-59821857	MS.gene042957:CDS	35.0%
	TTTGGATAAGAAGATGAGTC+AGG	-	chr6.4:59821837-59821856	MS.gene042957:CDS	35.0%
!	AATTTCCTCGCGTTTAAAAC+CGG	+	chr6.4:59821745-59821764	None:intergenic	35.0%
!!!	AGTTGGTTTTTTGTTCGAAC+CGG	+	chr6.4:59821716-59821735	None:intergenic	35.0%
	ACAGTCAAAAAACCAACAGC+CGG	-	chr6.4:59821694-59821713	MS.gene042957:CDS	40.0%
!	AAGGTCTTGTGCTTTAGGTT+TGG	+	chr6.4:59821623-59821642	None:intergenic	40.0%
!	GGAAAATGGAGAAGGTTTTG+GGG	-	chr6.4:59821947-59821966	MS.gene042957:CDS	40.0%
!	GGGAAAATGGAGAAGGTTTT+GGG	-	chr6.4:59821946-59821965	MS.gene042957:CDS	40.0%
!	GTTGTTCAGGAGTATGAGAA+TGG	-	chr6.4:59821895-59821914	MS.gene042957:CDS	40.0%
!	TTACGAAGGTCTTGTGCTTT+AGG	+	chr6.4:59821628-59821647	None:intergenic	40.0%
!!	GAAAATGGAGAAGGTTTTGG+GGG	-	chr6.4:59821948-59821967	MS.gene042957:CDS	40.0%
!!!	AGGTTTTGGGGGTTAAACTT+AGG	-	chr6.4:59821959-59821978	MS.gene042957:CDS	40.0%
!!!	GGTTTTGGGGGTTAAACTTA+GGG	-	chr6.4:59821960-59821979	MS.gene042957:CDS	40.0%
!!!	GTGCTTTAGGTTTGGTTTTG+TGG	+	chr6.4:59821615-59821634	None:intergenic	40.0%
!!!	GTTTTGGGGGTTAAACTTAG+GGG	-	chr6.4:59821961-59821980	MS.gene042957:CDS	40.0%
!!!	TCAAGGTGTTTTGGGGAAAA+TGG	-	chr6.4:59821933-59821952	MS.gene042957:CDS	40.0%
!!!	TGTTTTGGGGAAAATGGAGA+AGG	-	chr6.4:59821939-59821958	MS.gene042957:CDS	40.0%
	AAAACAAGACTGGGAACCAG+TGG	-	chr6.4:59821585-59821604	MS.gene042957:CDS	45.0%
	ACAAGACCTTCGTAACCCAA+AGG	-	chr6.4:59821633-59821652	MS.gene042957:CDS	45.0%
	ATTAGATGAAGCGGCTGAAC+CGG	-	chr6.4:59821762-59821781	MS.gene042957:CDS	45.0%
	ATTGTTCCTACGGTTCGTGT+CGG	+	chr6.4:59821565-59821584	None:intergenic	45.0%
	CAAAAAACCAACTTCCGGAC+CGG	-	chr6.4:59821723-59821742	MS.gene042957:CDS	45.0%
	CGCGAGGAAATTAGATGAAG+CGG	-	chr6.4:59821753-59821772	MS.gene042957:CDS	45.0%
	GAAGATGAGTCAGGGTGATT+TGG	-	chr6.4:59821846-59821865	MS.gene042957:CDS	45.0%
!	GGGGAAAATGGAGAAGGTTT+TGG	-	chr6.4:59821945-59821964	MS.gene042957:CDS	45.0%
!	TTATGGCCTTTGGGTTACGA+AGG	+	chr6.4:59821642-59821661	None:intergenic	45.0%
!	TTTAAAACCGGTCCGGAAGT+TGG	+	chr6.4:59821733-59821752	None:intergenic	45.0%
!	TTTTTGTTCGAACCGGCTGT+TGG	+	chr6.4:59821709-59821728	None:intergenic	45.0%
!!	TGAAAGGGTTCAGGTTGTTC+AGG	-	chr6.4:59821882-59821901	MS.gene042957:CDS	45.0%
!!!	TTGGTTTTGTGGAGAACCAC+TGG	+	chr6.4:59821604-59821623	None:intergenic	45.0%
	ATAAACCAAGCCCTCCGAAC+CGG	-	chr6.4:59821658-59821677	MS.gene042957:CDS	50.0%
	GACTGTTAAGACCTCTGCTC+CGG	+	chr6.4:59821680-59821699	None:intergenic	50.0%
	TTAAGACCTCTGCTCCGGTT+CGG	+	chr6.4:59821675-59821694	None:intergenic	50.0%
!	AGGGCTTGGTTTATGGCCTT+TGG	+	chr6.4:59821652-59821671	None:intergenic	50.0%
!	GGGCTTGGTTTATGGCCTTT+GGG	+	chr6.4:59821651-59821670	None:intergenic	50.0%
!	CCTCGCGTTTAAAACCGGTC+CGG	+	chr6.4:59821740-59821759	None:intergenic	55.0%
!	CGCGATACAGAAAGCGCGTT+TGG	-	chr6.4:59821819-59821838	MS.gene042957:CDS	55.0%
!	GGTTCGGAGGGCTTGGTTTA+TGG	+	chr6.4:59821659-59821678	None:intergenic	55.0%
	AGATGAAGCGGCTGAACCGG+CGG	-	chr6.4:59821765-59821784	MS.gene042957:CDS	60.0%
!!!	CCGGACCGGTTTTAAACGCG+AGG	-	chr6.4:59821737-59821756	MS.gene042957:CDS	60.0%
!!	AGACCTCTGCTCCGGTTCGG+AGG	+	chr6.4:59821672-59821691	None:intergenic	65.0%
!!	GACCTCTGCTCCGGTTCGGA+GGG	+	chr6.4:59821671-59821690	None:intergenic	65.0%
	AGCCCTCCGAACCGGAGCAG+AGG	-	chr6.4:59821666-59821685	MS.gene042957:CDS	70.0%
!	CTGAACCGGCGGCGTTGGAG+CGG	-	chr6.4:59821776-59821795	MS.gene042957:CDS	70.0%
!	TGAACCGGCGGCGTTGGAGC+GGG	-	chr6.4:59821777-59821796	MS.gene042957:CDS	70.0%
!!	CTGCTCCGGTTCGGAGGGCT+TGG	+	chr6.4:59821666-59821685	None:intergenic	70.0%
	AGCGGCTGAACCGGCGGCGT+TGG	-	chr6.4:59821771-59821790	MS.gene042957:CDS	75.0%
	CCAACCCGCTCCAACGCCGC+CGG	+	chr6.4:59821784-59821803	None:intergenic	75.0%
!!	GCGGCGTTGGAGCGGGTTGG+TGG	-	chr6.4:59821784-59821803	MS.gene042957:CDS	75.0%
!!	CCGGCGGCGTTGGAGCGGGT+TGG	-	chr6.4:59821781-59821800	MS.gene042957:CDS	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.4	gene	59821559	59821999	59821559	ID=MS.gene042957
chr6.4	mRNA	59821559	59821999	59821559	ID=MS.gene042957.t1;Parent=MS.gene042957
chr6.4	exon	59821559	59821999	59821559	ID=MS.gene042957.t1.exon1;Parent=MS.gene042957.t1
chr6.4	CDS	59821559	59821999	59821559	ID=cds.MS.gene042957.t1;Parent=MS.gene042957.t1

Gene Sequence

>MS.gene042957

ATGCCGACACGAACCGTAGGAACAATAAAACAAGACTGGGAACCAGTGGTTCTCCACAAAACCAAACCTAAAGCACAAGACCTTCGTAACCCAAAGGCCATAAACCAAGCCCTCCGAACCGGAGCAGAGGTCTTAACAGTCAAAAAACCAACAGCCGGTTCGAACAAAAAACCAACTTCCGGACCGGTTTTAAACGCGAGGAAATTAGATGAAGCGGCTGAACCGGCGGCGTTGGAGCGGGTTGGTGGAGAAGTGAGACACGCGATACAGAAAGCGCGTTTGGATAAGAAGATGAGTCAGGGTGATTTGGCGAAATTGATTAATGAAAGGGTTCAGGTTGTTCAGGAGTATGAGAATGGTAAAGCTGTTATTAATCAAGGTGTTTTGGGGAAAATGGAGAAGGTTTTGGGGGTTAAACTTAGGGGTAAAATTGGAAAATAA

Protein sequence

>MS.gene042957.t1

MPTRTVGTIKQDWEPVVLHKTKPKAQDLRNPKAINQALRTGAEVLTVKKPTAGSNKKPTSGPVLNARKLDEAAEPAALERVGGEVRHAIQKARLDKKMSQGDLAKLINERVQVVQEYENGKAVINQGVLGKMEKVLGVKLRGKIGK