AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene073154

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073154.t1 MTR_2g084220 97.857 140 3 0 1 140 1 140 1.06e-96 275
MS.gene073154.t1 MTR_4g080090 92.029 138 11 0 3 140 5 142 2.63e-83 241
MS.gene073154.t1 MTR_6g018330 75.182 137 18 1 4 140 23 143 6.44e-64 192
MS.gene073154.t1 MTR_6g086280 52.174 138 66 0 2 139 9 146 2.69e-45 145
MS.gene073154.t1 MTR_6g086280 52.555 137 65 0 3 139 10 146 2.77e-45 145
MS.gene073154.t1 MTR_1g047000 92.683 41 3 0 100 140 16 56 6.70e-21 80.1
MS.gene073154.t1 MTR_7g117675 95.000 40 2 0 90 129 1 40 2.94e-20 79.0
MS.gene073154.t1 MTR_4g103930 54.545 77 22 1 30 93 45 121 6.45e-17 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073154.t1 AT3G58680 80.435 138 27 0 3 140 5 142 6.64e-81 234
MS.gene073154.t1 AT2G42680 81.884 138 25 0 3 140 5 142 1.43e-80 234
MS.gene073154.t1 AT3G24500 51.079 139 66 1 3 139 7 145 2.25e-43 140
MS.gene073154.t1 AT3G24500 43.820 89 48 1 3 89 7 95 2.38e-16 69.7

Find 45 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCCAATGATCTGCTGATT+TGG 0.246748 2.2:-13548594 None:intergenic
TTTCTTCATGGTTTCGATAT+CGG 0.259177 2.2:-13545305 None:intergenic
ATCCTTCCTGGCAGCGGCGG+TGG 0.288753 2.2:-13545248 None:intergenic
GACAGCTTTGTCATCCTTCC+TGG 0.316434 2.2:-13545260 None:intergenic
GCACTTCATTGAACATTAAG+AGG 0.360745 2.2:+13546808 MS.gene073154:CDS
CAGGACCCGTTTCACAAGAT+TGG 0.366530 2.2:+13545197 MS.gene073154:CDS
GACTTGTTTGCGCAGATGAT+CGG 0.384244 2.2:+13548349 MS.gene073154:intron
GTGATCCAGGAGTACGAGTC+AGG 0.396136 2.2:+13548561 MS.gene073154:CDS
TCCTTCCTGGCAGCGGCGGT+GGG 0.408929 2.2:-13545247 None:intergenic
AGAAGCTCAACGTTTCTTCA+TGG 0.430879 2.2:-13545317 None:intergenic
TCATTTCTTCCCACGCAGTT+TGG 0.431740 2.2:-13548637 None:intergenic
AGGACCCGTTTCACAAGATT+GGG 0.440017 2.2:+13545198 MS.gene073154:CDS
GGCAAGTTGGAGAGAGCTCT+TGG 0.442294 2.2:+13548612 MS.gene073154:CDS
AAGCTCCCACCGCCGCTGCC+AGG 0.442991 2.2:+13545242 MS.gene073154:CDS
GTCAACGCCGCTCGTCGTGC+CGG 0.452741 2.2:+13545280 MS.gene073154:CDS
AGTAAGCTTCTTGTCCATCC+TGG 0.456590 2.2:-13548406 None:intergenic
ACTTGTTTGCGCAGATGATC+GGG 0.459972 2.2:+13548350 MS.gene073154:intron
CTTTCCCTGACTCGTACTCC+TGG 0.461120 2.2:-13548566 None:intergenic
ATTCCAAATCAGCAGATCAT+TGG 0.464427 2.2:+13548591 MS.gene073154:CDS
GGTTCCCAATCTTGTGAAAC+GGG 0.465547 2.2:-13545202 None:intergenic
GCTTTCTTGCGGAGGACGAC+GGG 0.478528 2.2:-13545223 None:intergenic
ATAGCTTTCTTGAGTTCAGT+TGG 0.482895 2.2:-13548375 None:intergenic
TAGCTTTCTTGAGTTCAGTT+GGG 0.492776 2.2:-13548374 None:intergenic
TGAGAAGCCTCAAGTGATCC+AGG 0.497642 2.2:+13548548 MS.gene073154:CDS
TCAGCAGATCATTGGCAAGT+TGG 0.510535 2.2:+13548599 MS.gene073154:CDS
GGGTTCCCAATCTTGTGAAA+CGG 0.516848 2.2:-13545203 None:intergenic
TCCCACCGCCGCTGCCAGGA+AGG 0.517927 2.2:+13545246 MS.gene073154:CDS
TTTGTCATCCTTCCTGGCAG+CGG 0.537196 2.2:-13545254 None:intergenic
AGAAAGCTATAATGCAAGCC+AGG 0.541759 2.2:+13548388 MS.gene073154:CDS
AGCTTTCTTGCGGAGGACGA+CGG 0.546703 2.2:-13545224 None:intergenic
AATGATCTGCTGATTTGGAA+TGG 0.551980 2.2:-13548589 None:intergenic
AGCTATAATGCAAGCCAGGA+TGG 0.555000 2.2:+13548392 MS.gene073154:CDS
CGGCGGTGGGAGCTTTCTTG+CGG 0.555292 2.2:-13545234 None:intergenic
CATGGTTTCGATATCGGCAC+CGG 0.557535 2.2:-13545299 None:intergenic
CTCTTGGTGCCAAACTGCGT+GGG 0.558179 2.2:+13548628 MS.gene073154:CDS
TCAAACCATTCAACCATGGC+AGG 0.558509 2.2:+13545178 None:intergenic
GTCATCCTTCCTGGCAGCGG+CGG 0.565962 2.2:-13545251 None:intergenic
CGGTGGGAGCTTTCTTGCGG+AGG 0.580235 2.2:-13545231 None:intergenic
TTGTGAAACGGGTCCTGCCA+TGG 0.588651 2.2:-13545191 None:intergenic
ATCGGCACCGGCACGACGAG+CGG 0.589916 2.2:-13545287 None:intergenic
GCTCTTGGTGCCAAACTGCG+TGG 0.608518 2.2:+13548627 MS.gene073154:CDS
GAACATTAAGAGGCTTGATG+AGG 0.616822 2.2:+13546818 MS.gene073154:CDS
TGATCCAGGAGTACGAGTCA+GGG 0.620671 2.2:+13548562 MS.gene073154:CDS
TCGTACTCCTGGATCACTTG+AGG 0.629262 2.2:-13548555 None:intergenic
GATGGACAAGAAGCTTACTC+AGG 0.656852 2.2:+13548410 MS.gene073154:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGTTTCAATATTATAAAAT+GGG - chr2.2:13546896-13546915 None:intergenic 10.0%
!!! AAATAATAAATGTTTTTTCT+TGG + chr2.2:13545569-13545588 MS.gene073154:intron 10.0%
!!! AAATTTATTTTAATAAACGA+AGG - chr2.2:13545413-13545432 None:intergenic 10.0%
!! AAAATTCATATTCTTTATCT+GGG + chr2.2:13547499-13547518 MS.gene073154:intron 15.0%
!! ATTGTTGATATTTCTTATTA+GGG + chr2.2:13545488-13545507 MS.gene073154:intron 15.0%
!! TTCTATTATAAATTATGAAG+AGG + chr2.2:13548013-13548032 MS.gene073154:intron 15.0%
!! TTGTAGAAAAATATTTGATT+AGG - chr2.2:13547960-13547979 None:intergenic 15.0%
!!! GTTGTTTCAATATTATAAAA+TGG - chr2.2:13546897-13546916 None:intergenic 15.0%
!! AAAAAAAATAGTAGAGTAAC+CGG - chr2.2:13546870-13546889 None:intergenic 20.0%
!! AAAAATATTTGATTAGGATG+GGG - chr2.2:13547954-13547973 None:intergenic 20.0%
!! AGAAAAATATTTGATTAGGA+TGG - chr2.2:13547956-13547975 None:intergenic 20.0%
!! ATATCTTCCATAATTAATTG+TGG + chr2.2:13547321-13547340 MS.gene073154:intron 20.0%
!! ATGAATTCCACAATTAATTA+TGG - chr2.2:13547331-13547350 None:intergenic 20.0%
!! CTATTAATATGATGTGTTTA+AGG + chr2.2:13545632-13545651 MS.gene073154:intron 20.0%
!! GAAAAATATTTGATTAGGAT+GGG - chr2.2:13547955-13547974 None:intergenic 20.0%
!! GAAAATTCATATTCTTTATC+TGG + chr2.2:13547498-13547517 MS.gene073154:intron 20.0%
!! GATTAAAGAAATGAAAGAAA+AGG - chr2.2:13545345-13545364 None:intergenic 20.0%
!! GATTGTTGATATTTCTTATT+AGG + chr2.2:13545487-13545506 MS.gene073154:intron 20.0%
!!! TAATTTGTTAAATCACTGTT+TGG + chr2.2:13545465-13545484 MS.gene073154:intron 20.0%
!!! TATGTTTTCATATGTTATCT+TGG + chr2.2:13545932-13545951 MS.gene073154:intron 20.0%
! ACTTATGTATTTACAAAGAG+AGG + chr2.2:13548162-13548181 MS.gene073154:intron 25.0%
! CATTGAAGCATAACATAATT+TGG + chr2.2:13547164-13547183 MS.gene073154:intron 25.0%
! CTTATGTATTTACAAAGAGA+GGG + chr2.2:13548163-13548182 MS.gene073154:intron 25.0%
! TATAACACGAGATAAAAGAA+AGG + chr2.2:13546220-13546239 MS.gene073154:intron 25.0%
! TATAATAGAATTGCATTTGC+AGG - chr2.2:13548003-13548022 None:intergenic 25.0%
! TCAAGACAAAAAAACATATG+TGG - chr2.2:13548472-13548491 None:intergenic 25.0%
! TTATGTATTTACAAAGAGAG+GGG + chr2.2:13548164-13548183 MS.gene073154:intron 25.0%
! TTTAATGTGGATATAACATG+TGG + chr2.2:13547370-13547389 MS.gene073154:intron 25.0%
! TTTCAATAATCCAGAGTTTA+GGG - chr2.2:13546083-13546102 None:intergenic 25.0%
!! ACAATATTCTTTTATTAGCC+TGG + chr2.2:13547399-13547418 MS.gene073154:intron 25.0%
!! ATGCATTTTTATAATTGCTG+TGG - chr2.2:13548054-13548073 None:intergenic 25.0%
!! TTCATATTCTTTTACTAGCT+TGG + chr2.2:13547346-13547365 MS.gene073154:intron 25.0%
!! TTTTCAATAATCCAGAGTTT+AGG - chr2.2:13546084-13546103 None:intergenic 25.0%
!!! GTTGTTTTCATAATCAATCT+TGG + chr2.2:13546407-13546426 MS.gene073154:intron 25.0%
AAAGTCCTACGTCAAATTTA+GGG - chr2.2:13546559-13546578 None:intergenic 30.0%
AAATTGAGCAAAGTAAATCC+AGG - chr2.2:13547420-13547439 None:intergenic 30.0%
AACCAACAAGATATATACAC+AGG - chr2.2:13546988-13547007 None:intergenic 30.0%
AATGGATCAAAACCTAAAAC+TGG - chr2.2:13548234-13548253 None:intergenic 30.0%
AATGTCCACCAATTATCAAA+GGG - chr2.2:13547701-13547720 None:intergenic 30.0%
ACCAACAAGATATATACACA+GGG - chr2.2:13546987-13547006 None:intergenic 30.0%
AGGTATGGCTCTTATATAAA+AGG - chr2.2:13548297-13548316 None:intergenic 30.0%
CCAAAAATGTCTTCATAGTT+TGG + chr2.2:13547629-13547648 MS.gene073154:intron 30.0%
GAATATCGTATGAAAGTTCA+TGG + chr2.2:13547442-13547461 MS.gene073154:intron 30.0%
GGTATGGCTCTTATATAAAA+GGG - chr2.2:13548296-13548315 None:intergenic 30.0%
TAATGTCCACCAATTATCAA+AGG - chr2.2:13547702-13547721 None:intergenic 30.0%
TGGAATATCATAAGACTGTT+TGG + chr2.2:13547283-13547302 MS.gene073154:intron 30.0%
TGTTGTTAAAGGGAATACAT+GGG - chr2.2:13547867-13547886 None:intergenic 30.0%
TTACCTATAGGTGTATTACT+AGG + chr2.2:13547243-13547262 MS.gene073154:intron 30.0%
TTCCACTAAAAATTCTAGTC+AGG - chr2.2:13548265-13548284 None:intergenic 30.0%
TTGAATGTAATCACATACGA+CGG + chr2.2:13545788-13545807 MS.gene073154:intron 30.0%
TTTCTTCATGGTTTCGATAT+CGG - chr2.2:13545308-13545327 None:intergenic 30.0%
! AAAAAGTTTTACTTCCACCA+AGG - chr2.2:13547212-13547231 None:intergenic 30.0%
! AAACAACATTTTGCAACTCT+TGG + chr2.2:13546910-13546929 MS.gene073154:intron 30.0%
! AACAACATTTTGCAACTCTT+GGG + chr2.2:13546911-13546930 MS.gene073154:intron 30.0%
! AATATGATGTGTTTAAGGCA+CGG + chr2.2:13545637-13545656 MS.gene073154:intron 30.0%
! CCAAACTATGAAGACATTTT+TGG - chr2.2:13547632-13547651 None:intergenic 30.0%
! GGATTTACTTTGCTCAATTT+TGG + chr2.2:13547420-13547439 MS.gene073154:intron 30.0%
! TATTACTAGGTGCTAACTTT+TGG + chr2.2:13547256-13547275 MS.gene073154:intron 30.0%
! TCTGAATCAGTGTTGTTAAA+GGG - chr2.2:13547877-13547896 None:intergenic 30.0%
! TTACTAGCTTGGATTTAATG+TGG + chr2.2:13547357-13547376 MS.gene073154:intron 30.0%
! TTCTAAGTGTTGTCAAATAG+TGG + chr2.2:13547124-13547143 MS.gene073154:intron 30.0%
! TTCTTGGTGATAATCTTTCA+TGG + chr2.2:13545585-13545604 MS.gene073154:intron 30.0%
!! ATAAGCATTCAAGTTGAAAG+AGG - chr2.2:13548317-13548336 None:intergenic 30.0%
!! ATCCTGACTAGAATTTTTAG+TGG + chr2.2:13548260-13548279 MS.gene073154:intron 30.0%
AAGCACTAGTCAGACTATTA+AGG - chr2.2:13546021-13546040 None:intergenic 35.0%
AAGCACTTGTATGACTATGA+AGG - chr2.2:13546497-13546516 None:intergenic 35.0%
ACACCTATAGGTAACTTCTA+TGG - chr2.2:13547237-13547256 None:intergenic 35.0%
ATAGCTTTCTTGAGTTCAGT+TGG - chr2.2:13548378-13548397 None:intergenic 35.0%
ATGTCCACCAATTATCAAAG+GGG - chr2.2:13547700-13547719 None:intergenic 35.0%
ATTATGAAGAGGTAGTAGTG+TGG + chr2.2:13548024-13548043 MS.gene073154:intron 35.0%
ATTCCAAATCAGCAGATCAT+TGG + chr2.2:13548591-13548610 MS.gene073154:CDS 35.0%
CAAAGTCCTACGTCAAATTT+AGG - chr2.2:13546560-13546579 None:intergenic 35.0%
CATATTCTTTATCTGGGCTT+GGG + chr2.2:13547505-13547524 MS.gene073154:intron 35.0%
CTAAATTTGACGTAGGACTT+TGG + chr2.2:13546558-13546577 MS.gene073154:intron 35.0%
GAACGAATTTCGAAATCCTA+GGG - chr2.2:13545391-13545410 None:intergenic 35.0%
GAATGCTTATACCTAGTAAC+TGG + chr2.2:13548327-13548346 MS.gene073154:intron 35.0%
GCACTTCATTGAACATTAAG+AGG + chr2.2:13546808-13546827 MS.gene073154:CDS 35.0%
GTTGTTAAAGGGAATACATG+GGG - chr2.2:13547866-13547885 None:intergenic 35.0%
TAGCTTTCTTGAGTTCAGTT+GGG - chr2.2:13548377-13548396 None:intergenic 35.0%
TCACCATAGAAGTTACCTAT+AGG + chr2.2:13547231-13547250 MS.gene073154:intron 35.0%
TCATATTCTTTATCTGGGCT+TGG + chr2.2:13547504-13547523 MS.gene073154:intron 35.0%
TCCCTGTGTATATATCTTGT+TGG + chr2.2:13546983-13547002 MS.gene073154:intron 35.0%
TCTAGTCAGGATAGAGAAAA+TGG - chr2.2:13548252-13548271 None:intergenic 35.0%
TGTATGTATTCCTAATGTCC+TGG + chr2.2:13546956-13546975 MS.gene073154:intron 35.0%
TTGAATGTAACCACATGTGT+TGG + chr2.2:13546114-13546133 MS.gene073154:intron 35.0%
! AATTTAGGGCATGTTTGGAT+TGG - chr2.2:13546545-13546564 None:intergenic 35.0%
! ACAACATTTTGCAACTCTTG+GGG + chr2.2:13546912-13546931 MS.gene073154:intron 35.0%
! AGGTTAGTGCTGTTAAATAG+CGG + chr2.2:13547029-13547048 MS.gene073154:intron 35.0%
! CTACTATTTTCTGCAATCCT+TGG + chr2.2:13547192-13547211 MS.gene073154:intron 35.0%
! CTCTGAATCAGTGTTGTTAA+AGG - chr2.2:13547878-13547897 None:intergenic 35.0%
! GTGTTGTTAAAGGGAATACA+TGG - chr2.2:13547868-13547887 None:intergenic 35.0%
! TCTGCTGTTATACCAGTTTT+AGG + chr2.2:13548219-13548238 MS.gene073154:intron 35.0%
! TTCTAAGTATCGTTAACCCT+AGG + chr2.2:13545372-13545391 MS.gene073154:intron 35.0%
!! AATGATCTGCTGATTTGGAA+TGG - chr2.2:13548592-13548611 None:intergenic 35.0%
!! CATTCAAGTTGAAAGAGGTA+TGG - chr2.2:13548312-13548331 None:intergenic 35.0%
!!! AACTTACTGTTGTCGTTTTG+TGG + chr2.2:13547089-13547108 MS.gene073154:intron 35.0%
!!! AGGTGCTAACTTTTGGAATT+TGG + chr2.2:13547263-13547282 MS.gene073154:intron 35.0%
!!! GAAATTTTTTTGACAGCCTC+CGG - chr2.2:13547833-13547852 None:intergenic 35.0%
!!! GCTGTTTTTGTAAGCTATCT+TGG + chr2.2:13546271-13546290 MS.gene073154:intron 35.0%
AAAGGGAATACATGGGGAAA+TGG - chr2.2:13547860-13547879 None:intergenic 40.0%
ACATATGTGGTGAGTGAAGT+CGG - chr2.2:13548459-13548478 None:intergenic 40.0%
ACATGCCCTAAATTTGACGT+AGG + chr2.2:13546551-13546570 MS.gene073154:intron 40.0%
AGAAAGCTATAATGCAAGCC+AGG + chr2.2:13548388-13548407 MS.gene073154:CDS 40.0%
AGAAGCTCAACGTTTCTTCA+TGG - chr2.2:13545320-13545339 None:intergenic 40.0%
ATGCCTTCGATCATAAGTGT+TGG + chr2.2:13546172-13546191 MS.gene073154:intron 40.0%
ATTTAACAGCACTAACCTCC+AGG - chr2.2:13547027-13547046 None:intergenic 40.0%
GAACATTAAGAGGCTTGATG+AGG + chr2.2:13546818-13546837 MS.gene073154:CDS 40.0%
GACGTAGGACTTTGGATTAT+TGG + chr2.2:13546566-13546585 MS.gene073154:intron 40.0%
GCACCTAGTAATACACCTAT+AGG - chr2.2:13547249-13547268 None:intergenic 40.0%
GCCATAGCACTATAGTATAG+CGG + chr2.2:13547061-13547080 MS.gene073154:intron 40.0%
GGAACGAATTTCGAAATCCT+AGG - chr2.2:13545392-13545411 None:intergenic 40.0%
GTCTAGATTAAGAGCAGTGT+TGG + chr2.2:13547722-13547741 MS.gene073154:intron 40.0%
GTGTCAGTGCTAGAAAATGT+TGG + chr2.2:13546140-13546159 MS.gene073154:intron 40.0%
TATATACACAGGGAAACACC+AGG - chr2.2:13546977-13546996 None:intergenic 40.0%
TCCGCTATACTATAGTGCTA+TGG - chr2.2:13547065-13547084 None:intergenic 40.0%
TGTCCACCAATTATCAAAGG+GGG - chr2.2:13547699-13547718 None:intergenic 40.0%
! AACATTTTGCAACTCTTGGG+GGG + chr2.2:13546914-13546933 MS.gene073154:intron 40.0%
! AGTTTAGGGCATGATTGGAT+TGG - chr2.2:13546069-13546088 None:intergenic 40.0%
! ATACCCCCTTTGATAATTGG+TGG + chr2.2:13547693-13547712 MS.gene073154:intron 40.0%
! CAACATTTTGCAACTCTTGG+GGG + chr2.2:13546913-13546932 MS.gene073154:intron 40.0%
! CCAATACCCCCTTTGATAAT+TGG + chr2.2:13547690-13547709 MS.gene073154:intron 40.0%
! CCAATTATCAAAGGGGGTAT+TGG - chr2.2:13547693-13547712 None:intergenic 40.0%
! CGTCAAATTTAGGGCATGTT+TGG - chr2.2:13546550-13546569 None:intergenic 40.0%
! CTATTTTCTGCAATCCTTGG+TGG + chr2.2:13547195-13547214 MS.gene073154:intron 40.0%
! TTATGATCGAAGGCATGTCT+GGG - chr2.2:13546168-13546187 None:intergenic 40.0%
! TTGCCAATGATCTGCTGATT+TGG - chr2.2:13548597-13548616 None:intergenic 40.0%
!! ATTAAGAGCAGTGTTGGCAA+TGG + chr2.2:13547728-13547747 MS.gene073154:intron 40.0%
!!! TGTTGTCGTTTTGTGGTACA+TGG + chr2.2:13547096-13547115 MS.gene073154:intron 40.0%
ACTTGTTTGCGCAGATGATC+GGG + chr2.2:13548350-13548369 MS.gene073154:intron 45.0%
AGCTATAATGCAAGCCAGGA+TGG + chr2.2:13548392-13548411 MS.gene073154:CDS 45.0%
AGGACCCGTTTCACAAGATT+GGG + chr2.2:13545198-13545217 MS.gene073154:CDS 45.0%
AGTAAGCTTCTTGTCCATCC+TGG - chr2.2:13548409-13548428 None:intergenic 45.0%
ATTCCTTCTCAAATTGCCGG+AGG + chr2.2:13547814-13547833 MS.gene073154:intron 45.0%
CGCAAACAAGTCCAGTTACT+AGG - chr2.2:13548341-13548360 None:intergenic 45.0%
GATGGACAAGAAGCTTACTC+AGG + chr2.2:13548410-13548429 MS.gene073154:CDS 45.0%
GCACCAACACTTATGATCGA+AGG - chr2.2:13546178-13546197 None:intergenic 45.0%
GCATTTGCAGGCAACAAGAT+AGG - chr2.2:13547991-13548010 None:intergenic 45.0%
GGCATTCCTTCTCAAATTGC+CGG + chr2.2:13547811-13547830 MS.gene073154:intron 45.0%
GGGTTCCCAATCTTGTGAAA+CGG - chr2.2:13545206-13545225 None:intergenic 45.0%
GGTTCCCAATCTTGTGAAAC+GGG - chr2.2:13545205-13545224 None:intergenic 45.0%
TCAGCACTCTGACCAGAAAA+TGG - chr2.2:13546727-13546746 None:intergenic 45.0%
TCAGCAGATCATTGGCAAGT+TGG + chr2.2:13548599-13548618 MS.gene073154:CDS 45.0%
TCATTTCTTCCCACGCAGTT+TGG - chr2.2:13548640-13548659 None:intergenic 45.0%
TCCAATCATGCCCTAAACTC+TGG + chr2.2:13546070-13546089 MS.gene073154:intron 45.0%
! ATGCCTTCGATCAGAAGTGT+TGG + chr2.2:13546311-13546330 MS.gene073154:intron 45.0%
! CTTATGATCGAAGGCATGTC+TGG - chr2.2:13546169-13546188 None:intergenic 45.0%
! GAATTTAGCTCGTGAGTCTC+CGG + chr2.2:13546848-13546867 MS.gene073154:intron 45.0%
! GACTTGTTTGCGCAGATGAT+CGG + chr2.2:13548349-13548368 MS.gene073154:intron 45.0%
! GATGTGTTTAAGGCACGGTA+TGG + chr2.2:13545642-13545661 MS.gene073154:intron 45.0%
! TATGATCGAAGGCATGTCTG+GGG - chr2.2:13546167-13546186 None:intergenic 45.0%
! TCCAGAGTTTAGGGCATGAT+TGG - chr2.2:13546074-13546093 None:intergenic 45.0%
!! GTCAGAGTGCTGATTGTGTT+GGG + chr2.2:13546734-13546753 MS.gene073154:intron 45.0%
!! GTGTTCATATGGCGGAGTTT+TGG - chr2.2:13547761-13547780 None:intergenic 45.0%
!!! CACTGATTCGCTCCATTTTC+TGG + chr2.2:13546712-13546731 MS.gene073154:intron 45.0%
!!! ATTTTTTTTTAAATTATTGT+CGG - chr2.2:13545746-13545765 None:intergenic 5.0%
ATCTGGGCTTGGGATTACTG+TGG + chr2.2:13547515-13547534 MS.gene073154:intron 50.0%
CAGGACCCGTTTCACAAGAT+TGG + chr2.2:13545197-13545216 MS.gene073154:CDS 50.0%
CAGGGAAACACCAGGACATT+AGG - chr2.2:13546969-13546988 None:intergenic 50.0%
CATGGTTTCGATATCGGCAC+CGG - chr2.2:13545302-13545321 None:intergenic 50.0%
CTGACACACACCAACACATG+TGG - chr2.2:13546127-13546146 None:intergenic 50.0%
GCTCCAACACTTCTGATCGA+AGG - chr2.2:13546317-13546336 None:intergenic 50.0%
TCGTACTCCTGGATCACTTG+AGG - chr2.2:13548558-13548577 None:intergenic 50.0%
TGAGAAGCCTCAAGTGATCC+AGG + chr2.2:13548548-13548567 MS.gene073154:CDS 50.0%
TTTGTCATCCTTCCTGGCAG+CGG - chr2.2:13545257-13545276 None:intergenic 50.0%
! ACGCCTTCGATCAGAAGTGT+CGG + chr2.2:13545841-13545860 MS.gene073154:intron 50.0%
! GACAGCTTTGTCATCCTTCC+TGG - chr2.2:13545263-13545282 None:intergenic 50.0%
! TGATCCAGGAGTACGAGTCA+GGG + chr2.2:13548562-13548581 MS.gene073154:CDS 50.0%
! TTGAAGCATGCGAGTTGACC+TGG + chr2.2:13547006-13547025 MS.gene073154:intron 50.0%
!! AAGAGCAGTGTTGGCAATGG+TGG + chr2.2:13547731-13547750 MS.gene073154:intron 50.0%
!! GGTCAGAGTGCTGATTGTGT+TGG + chr2.2:13546733-13546752 MS.gene073154:intron 50.0%
AGCTTTCTTGCGGAGGACGA+CGG - chr2.2:13545227-13545246 None:intergenic 55.0%
CAGCCTCCGGCAATTTGAGA+AGG - chr2.2:13547820-13547839 None:intergenic 55.0%
GCCATATGAACACGCCATGG+CGG + chr2.2:13547768-13547787 MS.gene073154:intron 55.0%
GCCATGGCGTGTTCATATGG+CGG - chr2.2:13547769-13547788 None:intergenic 55.0%
TCCGCCATATGAACACGCCA+TGG + chr2.2:13547765-13547784 MS.gene073154:intron 55.0%
! AAGCATGCGAGTTGACCTGG+AGG + chr2.2:13547009-13547028 MS.gene073154:intron 55.0%
! CTTCTGATCGAAGGCGTGTC+TGG - chr2.2:13545838-13545857 None:intergenic 55.0%
! CTTTCCCTGACTCGTACTCC+TGG - chr2.2:13548569-13548588 None:intergenic 55.0%
! GCACCGACACTTCTGATCGA+AGG - chr2.2:13545847-13545866 None:intergenic 55.0%
! GGCAAGTTGGAGAGAGCTCT+TGG + chr2.2:13548612-13548631 MS.gene073154:CDS 55.0%
! GTGATCCAGGAGTACGAGTC+AGG + chr2.2:13548561-13548580 MS.gene073154:CDS 55.0%
!! CTCTTGGTGCCAAACTGCGT+GGG + chr2.2:13548628-13548647 MS.gene073154:CDS 55.0%
!! GCAGTGTTGGCAATGGTGGA+AGG + chr2.2:13547735-13547754 MS.gene073154:intron 55.0%
!! GCCTATGGTGCTGCTATGGT+GGG + chr2.2:13547790-13547809 MS.gene073154:intron 55.0%
GCCCACCATAGCAGCACCAT+AGG - chr2.2:13547794-13547813 None:intergenic 60.0%
GCCGCCATGGCGTGTTCATA+TGG - chr2.2:13547772-13547791 None:intergenic 60.0%
GCTTTCTTGCGGAGGACGAC+GGG - chr2.2:13545226-13545245 None:intergenic 60.0%
!! GCTCTTGGTGCCAAACTGCG+TGG + chr2.2:13548627-13548646 MS.gene073154:CDS 60.0%
!! GGCCTATGGTGCTGCTATGG+TGG + chr2.2:13547789-13547808 MS.gene073154:intron 60.0%
AGCAGCACCATAGGCCGCCA+TGG - chr2.2:13547785-13547804 None:intergenic 65.0%
CGGCGGTGGGAGCTTTCTTG+CGG - chr2.2:13545237-13545256 None:intergenic 65.0%
CGGTGGGAGCTTTCTTGCGG+AGG - chr2.2:13545234-13545253 None:intergenic 65.0%
GAACACGCCATGGCGGCCTA+TGG + chr2.2:13547775-13547794 MS.gene073154:intron 65.0%
GTCATCCTTCCTGGCAGCGG+CGG - chr2.2:13545254-13545273 None:intergenic 65.0%
!! GGCGGCCTATGGTGCTGCTA+TGG + chr2.2:13547786-13547805 MS.gene073154:intron 65.0%
ATCCTTCCTGGCAGCGGCGG+TGG - chr2.2:13545251-13545270 None:intergenic 70.0%
GTCAACGCCGCTCGTCGTGC+CGG + chr2.2:13545280-13545299 MS.gene073154:CDS 70.0%
TCCTTCCTGGCAGCGGCGGT+GGG - chr2.2:13545250-13545269 None:intergenic 70.0%
!! ATCGGCACCGGCACGACGAG+CGG - chr2.2:13545290-13545309 None:intergenic 70.0%
AAGCTCCCACCGCCGCTGCC+AGG + chr2.2:13545242-13545261 MS.gene073154:CDS 75.0%
TCCCACCGCCGCTGCCAGGA+AGG + chr2.2:13545246-13545265 MS.gene073154:CDS 75.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 13545193 13548659 13545193 ID=MS.gene073154
chr2.2 mRNA 13545193 13548659 13545193 ID=MS.gene073154.t1;Parent=MS.gene073154
chr2.2 exon 13545193 13545328 13545193 ID=MS.gene073154.t1.exon1;Parent=MS.gene073154.t1
chr2.2 CDS 13545193 13545328 13545193 ID=cds.MS.gene073154.t1;Parent=MS.gene073154.t1
chr2.2 exon 13546775 13546858 13546775 ID=MS.gene073154.t1.exon2;Parent=MS.gene073154.t1
chr2.2 CDS 13546775 13546858 13546775 ID=cds.MS.gene073154.t1;Parent=MS.gene073154.t1
chr2.2 exon 13548364 13548446 13548364 ID=MS.gene073154.t1.exon3;Parent=MS.gene073154.t1
chr2.2 CDS 13548364 13548446 13548364 ID=cds.MS.gene073154.t1;Parent=MS.gene073154.t1
chr2.2 exon 13548540 13548659 13548540 ID=MS.gene073154.t1.exon4;Parent=MS.gene073154.t1
chr2.2 CDS 13548540 13548659 13548540 ID=cds.MS.gene073154.t1;Parent=MS.gene073154.t1
Gene Sequence

>MS.gene073154

ATGGCAGGACCCGTTTCACAAGATTGGGAACCCGTCGTCCTCCGCAAGAAAGCTCCCACCGCCGCTGCCAGGAAGGATGACAAAGCTGTCAACGCCGCTCGTCGTGCCGGTGCCGATATCGAAACCATGAAGAAACGTTGAGCTTCTTTCCTTTTCTTTCATTTCTTTAATCATTCGTTTTCTAAGTATCGTTAACCCTAGGATTTCGAAATTCGTTCCTTCGTTTATTAAAATAAATTTCATGTTATCTTTGTGCTTTCGTTCAAAGCGTTTAATTTGTTAAATCACTGTTTGGATTGTTGATATTTCTTATTAGGGTTTGTGTTAATTTTTTTTATATTGCATTTTTTTATTTGTTATATCTATGTTAAATTTGAAATAATAAATGTTTTTTCTTGGTGATAATCTTTCATGGTTTTTCATTTCTGTTGTTGATTTTCTATTAATATGATGTGTTTAAGGCACGGTATGGATAAACAGCTTAATTTACAGCTTATAGCACAAACACATCTCATGATAAGCACTTATGTTTAAACAACGACAAGTGACACCGACAATAATTTAAAAAAAAATAAAATAAAAGAAGAAGTGATAATTGAATGTAATCACATACGACGGCGTGTGATGCTGCAAAAACTGACACCAGACACGCCTTCGATCAGAAGTGTCGGTGCTATGTAGTGTGTAAGCTATTTCTATAACACGAGATAAAATAAAGTCACATTGTTTTCGTATAAGCTATGTTTTCATATGTTATCTTGGAGAGCTTATGAAAATAAGCTGAAACAACTTATGAACAAGTCATCAGCTGTTTTTATAAGCTCTCCTTAATAGTCTGACTAGTGCTTATGCTATTATATAAGCTCAAATAAGCCAATCCAATCATGCCCTAAACTCTGGATTATTGAAAAAAAATGAGAATTGAATGTAACCACATGTGTTGGTGTGTGTCAGTGCTAGAAAATGTTGGACCCCAGACATGCCTTCGATCATAAGTGTTGGTGCTACATATTGTATAAGCTAGTTCTATAACACGAGATAAAAGAAAGGCACATTTTTTTTTTCGTATAAGCTATTAGCTGTTTTTGTAAGCTATCTTGGAGAGTTTATGAAAATAAATGCCTTCGATCAGAAGTGTTGGAGCTACATAGTGTATAAGCTATTTCTATAACACGAGATAAATTAAATTCACATTGATTTCGTATAATCTATGAGTTGTTTTCATAATCAATCTTGGATAGCTTATGAAAATAAGCTGAAAACAACTCATGAACAAGTCATCAGCTGTTTTCATAAGTTCTCCTTCATAGTCATACAAGTGCTTATGCTATTATATAAGCTCAAATAAGCCAATCCAAACATGCCCTAAATTTGACGTAGGACTTTGGATTATTGGATTTTCAAGACTCTAACACAAATAGTTTATGAGCAGTTTTCTGCAAGTTGTTTTTGTAATTATGAGCAGTTTTTTTCGTTGATTGATCTTTTACTTGTATAATTGAATGTTTCTCATTTTTATCACTGATTCGCTCCATTTTCTGGTCAGAGTGCTGATTGTGTTGGGTTGTTCTATACATTACAGATAATGCTGCGACAAACAAAGCTGCTTCTAGCAGCACTTCATTGAACATTAAGAGGCTTGATGAGGATACAGAGAATTTAGCTCGTGAGTCTCCGGTTACTCTACTATTTTTTTTCTTCCCATTTTATAATATTGAAACAACATTTTGCAACTCTTGGGGGGTTTAACTTTTACAATGTATTGTATGTATTCCTAATGTCCTGGTGTTTCCCTGTGTATATATCTTGTTGGTTGAAGCATGCGAGTTGACCTGGAGGTTAGTGCTGTTAAATAGCGGTATAGTAGTGCCATAGCACTATAGTATAGCGGAATTTAACTTACTGTTGTCGTTTTGTGGTACATGGTTTAGTTCTAAGTGTTGTCAAATAGTGGCTATAGCTGTGACATAACATTGAAGCATAACATAATTTGGACAAACTACTATTTTCTGCAATCCTTGGTGGAAGTAAAACTTTTTCACCATAGAAGTTACCTATAGGTGTATTACTAGGTGCTAACTTTTGGAATTTGGAATATCATAAGACTGTTTGGAAGTGCTATATGTATATATCTTCCATAATTAATTGTGGAATTCATATTCTTTTACTAGCTTGGATTTAATGTGGATATAACATGTGGTTTTTTACAATATTCTTTTATTAGCCTGGATTTACTTTGCTCAATTTTGGAATATCGTATGAAAGTTCATGGTATTGTTATCTGCAATTATTACATAATTACTGAGAAAATTCATATTCTTTATCTGGGCTTGGGATTACTGTGGACATGTGTACTCTTTGTTGTTTGATTTCATATCTCAGATTTTTGTTATATTAATATTATATATGTTTACAATAATTCTTTTTATCACAATGCCAAAAATGTCTTCATAGTTTGGACATGAGAATGTGTGACTGTACTGTGTTCTAGTGTAGTCCAATACCCCCTTTGATAATTGGTGGACATTAGTCTAGATTAAGAGCAGTGTTGGCAATGGTGGAAGGCCAAAACTCCGCCATATGAACACGCCATGGCGGCCTATGGTGCTGCTATGGTGGGCATTCCTTCTCAAATTGCCGGAGGCTGTCAAAAAAATTTCAATGCCATTTCCCCATGTATTCCCTTTAACAACACTGATTCAGAGATCAGAGCTCTCGAGACTGATTCAGATCTGATTTAGAGCTTTATAATTGTAATCCCCATCCTAATCAAATATTTTTCTACAAATTGATATCCTATCTTGTTGCCTGCAAATGCAATTCTATTATAAATTATGAAGAGGTAGTAGTGTGGTCTACCACAGCAATTATAAAAATGCATCTCAGTTTACAATGTAGAATCTGTTGCTTGTGCTTTATTTCTTTTTGACGAATGACAAAATAGAAATTTGTAGTGTTCAGCAATTCAAACTTATGTATTTACAAAGAGAGGGGCTTTGTTTAACAGAGCAGTGTAGCATGCTAGCTCTGCTGTTATACCAGTTTTAGGTTTTGATCCATTTTCTCTATCCTGACTAGAATTTTTAGTGGAACATCAACTCCCTTTTATATAAGAGCCATACCTCTTTCAACTTGAATGCTTATACCTAGTAACTGGACTTGTTTGCGCAGATGATCGGGTCCCAACTGAACTCAAGAAAGCTATAATGCAAGCCAGGATGGACAAGAAGCTTACTCAGGCACAACTTGCTCAAGTATTGCTGCCGACTTCACTCACCACATATGTTTTTTTGTCTTGATTTCATTATTTGAAGTTTGATATTAATAACGAGCATGTTTGATTGCAGATTATCAATGAGAAGCCTCAAGTGATCCAGGAGTACGAGTCAGGGAAAGCCATTCCAAATCAGCAGATCATTGGCAAGTTGGAGAGAGCTCTTGGTGCCAAACTGCGTGGGAAGAAATGA
Protein sequence

>MS.gene073154.t1

MAGPVSQDWEPVVLRKKAPTAAARKDDKAVNAARRAGADIETMKKHNAATNKAASSSTSLNIKRLDEDTENLAHDRVPTELKKAIMQARMDKKLTQAQLAQIINEKPQVIQEYESGKAIPNQQIIGKLERALGAKLRGKK