AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene94585

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94585.t1 MTR_2g084220 98.571 140 2 0 1 140 1 140 1.19e-97 277
MS.gene94585.t1 MTR_4g080090 92.754 138 10 0 3 140 5 142 2.66e-84 243
MS.gene94585.t1 MTR_6g018330 75.182 137 18 1 4 140 23 143 9.05e-64 191
MS.gene94585.t1 MTR_6g086280 52.174 138 66 0 2 139 9 146 1.48e-45 145
MS.gene94585.t1 MTR_6g086280 52.174 138 66 0 2 139 9 146 1.59e-45 145
MS.gene94585.t1 MTR_1g047000 92.683 41 3 0 100 140 16 56 5.88e-21 80.5
MS.gene94585.t1 MTR_7g117675 95.000 40 2 0 90 129 1 40 2.44e-20 79.3
MS.gene94585.t1 MTR_4g103930 55.844 77 21 1 30 93 45 121 6.40e-18 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94585.t1 AT3G58680 81.159 138 26 0 3 140 5 142 7.25e-82 237
MS.gene94585.t1 AT2G42680 82.609 138 24 0 3 140 5 142 1.99e-81 236
MS.gene94585.t1 AT3G24500 51.799 139 65 1 3 139 7 145 2.32e-44 142
MS.gene94585.t1 AT3G24500 44.944 89 47 1 3 89 7 95 3.41e-17 72.0

Find 45 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCCAATGATCTGCTGATT+TGG 0.246748 2.4:-15384686 None:intergenic
TTTCTTCATGGTTTCGATAT+CGG 0.259177 2.4:-15381401 None:intergenic
ATCCTTCCTGGCAGCGGCGG+TGG 0.288753 2.4:-15381344 None:intergenic
GACAGCTTTGTCATCCTTCC+TGG 0.316434 2.4:-15381356 None:intergenic
CAGGACCCGTTTCACAAGAT+TGG 0.366530 2.4:+15381293 MS.gene94585:CDS
GACTTGTTTGCGCAGATGAT+CGG 0.384244 2.4:+15384440 MS.gene94585:intron
GTGATCCAGGAGTACGAGTC+AGG 0.396136 2.4:+15384653 MS.gene94585:CDS
TCCTTCCTGGCAGCGGCGGT+GGG 0.408929 2.4:-15381343 None:intergenic
GGCTCCCAATCTTGTGAAAC+GGG 0.430225 2.4:-15381298 None:intergenic
AGAAGCTCAACGTTTCTTCA+TGG 0.430879 2.4:-15381413 None:intergenic
TCATTTCTTCCCACGCAGTT+TGG 0.431740 2.4:-15384729 None:intergenic
GCACTTCATTGAACACTAAG+AGG 0.438741 2.4:+15382899 MS.gene94585:CDS
GGCAAGTTGGAGAGAGCTCT+TGG 0.442294 2.4:+15384704 MS.gene94585:CDS
AAGCTCCCACCGCCGCTGCC+AGG 0.442991 2.4:+15381338 MS.gene94585:CDS
AGGACCCGTTTCACAAGATT+GGG 0.445033 2.4:+15381294 MS.gene94585:CDS
GTCAACGCCGCTCGTCGTGC+CGG 0.452741 2.4:+15381376 MS.gene94585:CDS
AGTAAGCTTCTTGTCCATCC+TGG 0.456590 2.4:-15384497 None:intergenic
GCTTTCTTGCGGAGGACGAC+GGG 0.457580 2.4:-15381319 None:intergenic
ACTTGTTTGCGCAGATGATC+GGG 0.459972 2.4:+15384441 MS.gene94585:intron
CTTTCCCTGACTCGTACTCC+TGG 0.461120 2.4:-15384658 None:intergenic
ATTCCAAATCAGCAGATCAT+TGG 0.464427 2.4:+15384683 MS.gene94585:CDS
ATAGCTTTCTTGAGTTCAGT+TGG 0.482895 2.4:-15384466 None:intergenic
TAGCTTTCTTGAGTTCAGTT+GGG 0.492776 2.4:-15384465 None:intergenic
TGAGAAGCCTCAAGTGATCC+AGG 0.497642 2.4:+15384640 MS.gene94585:CDS
TCAGCAGATCATTGGCAAGT+TGG 0.510535 2.4:+15384691 MS.gene94585:CDS
GGGCTCCCAATCTTGTGAAA+CGG 0.514365 2.4:-15381299 None:intergenic
TCCCACCGCCGCTGCCAGGA+AGG 0.517927 2.4:+15381342 MS.gene94585:CDS
AGCTTTCTTGCGGAGGACGA+CGG 0.519594 2.4:-15381320 None:intergenic
TTTGTCATCCTTCCTGGCAG+CGG 0.537196 2.4:-15381350 None:intergenic
AGAAAGCTATAATGCAAGCC+AGG 0.541759 2.4:+15384479 MS.gene94585:CDS
AATGATCTGCTGATTTGGAA+TGG 0.551980 2.4:-15384681 None:intergenic
AGCTATAATGCAAGCCAGGA+TGG 0.555000 2.4:+15384483 MS.gene94585:CDS
CGGCGGTGGGAGCTTTCTTG+CGG 0.555292 2.4:-15381330 None:intergenic
CATGGTTTCGATATCGGCAC+CGG 0.557535 2.4:-15381395 None:intergenic
CTCTTGGTGCCAAACTGCGT+GGG 0.558179 2.4:+15384720 MS.gene94585:CDS
TCAAACCATTCAACCATGGC+AGG 0.558509 2.4:+15381274 None:intergenic
GTCATCCTTCCTGGCAGCGG+CGG 0.565962 2.4:-15381347 None:intergenic
CGGTGGGAGCTTTCTTGCGG+AGG 0.580235 2.4:-15381327 None:intergenic
TTGTGAAACGGGTCCTGCCA+TGG 0.588651 2.4:-15381287 None:intergenic
ATCGGCACCGGCACGACGAG+CGG 0.589916 2.4:-15381383 None:intergenic
GCTCTTGGTGCCAAACTGCG+TGG 0.608518 2.4:+15384719 MS.gene94585:CDS
TGATCCAGGAGTACGAGTCA+GGG 0.620671 2.4:+15384654 MS.gene94585:CDS
TCGTACTCCTGGATCACTTG+AGG 0.629262 2.4:-15384647 None:intergenic
GAACACTAAGAGGCTTGATG+AGG 0.646364 2.4:+15382909 MS.gene94585:CDS
GATGGACAAGAAGCTTACTC+AGG 0.656852 2.4:+15384501 MS.gene94585:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGTTTCAATATTATAAAAT+GGG - chr2.4:15382987-15383006 None:intergenic 10.0%
!!! AAATAATAAATGTTTTTTCT+TGG + chr2.4:15381669-15381688 MS.gene94585:intron 10.0%
!!! AATTTATTTTTAATAAACGA+AGG - chr2.4:15381509-15381528 None:intergenic 10.0%
!!! TTTTTTTTTCAAATTATTGT+CGG - chr2.4:15381846-15381865 None:intergenic 10.0%
!! AAAATTCATATTCTTTATCT+GGG + chr2.4:15383590-15383609 MS.gene94585:intron 15.0%
!! ATTGTTGATATTTCTTATTA+GGG + chr2.4:15381585-15381604 MS.gene94585:intron 15.0%
!! TTCTATTATAAATTATGAAG+AGG + chr2.4:15384104-15384123 MS.gene94585:intron 15.0%
!! TTGTAGAAAAATATTTGATT+AGG - chr2.4:15384051-15384070 None:intergenic 15.0%
!!! GTTGTTTCAATATTATAAAA+TGG - chr2.4:15382988-15383007 None:intergenic 15.0%
!! AAAAATATTTGATTAGGATG+GGG - chr2.4:15384045-15384064 None:intergenic 20.0%
!! AGAAAAATATTTGATTAGGA+TGG - chr2.4:15384047-15384066 None:intergenic 20.0%
!! ATATCTTCCATAATTAATTG+TGG + chr2.4:15383412-15383431 MS.gene94585:intron 20.0%
!! ATGAATTCCACAATTAATTA+TGG - chr2.4:15383422-15383441 None:intergenic 20.0%
!! CTATTAATATGATGTGTTTA+AGG + chr2.4:15381732-15381751 MS.gene94585:intron 20.0%
!! GAAAAAAATAATAGAGTAAC+CGG - chr2.4:15382961-15382980 None:intergenic 20.0%
!! GAAAAATATTTGATTAGGAT+GGG - chr2.4:15384046-15384065 None:intergenic 20.0%
!! GAAAATTCATATTCTTTATC+TGG + chr2.4:15383589-15383608 MS.gene94585:intron 20.0%
!! GATTAAAGAAATGAAAGAAA+AGG - chr2.4:15381441-15381460 None:intergenic 20.0%
!! GATTGTTGATATTTCTTATT+AGG + chr2.4:15381584-15381603 MS.gene94585:intron 20.0%
!! TTATCTATAGATGTATTACT+AGG + chr2.4:15383334-15383353 MS.gene94585:intron 20.0%
!! TTCAATATTATAAAATGGGA+AGG - chr2.4:15382983-15383002 None:intergenic 20.0%
!!! TAATTTGTTAAATCACTGTT+TGG + chr2.4:15381562-15381581 MS.gene94585:intron 20.0%
!!! TATGTTTTCATATGTTATCT+TGG + chr2.4:15382020-15382039 MS.gene94585:intron 20.0%
! ACATCTATAGATAACTTCTA+TGG - chr2.4:15383328-15383347 None:intergenic 25.0%
! ACTTATGTATTTACAAAGAG+AGG + chr2.4:15384253-15384272 MS.gene94585:intron 25.0%
! CAAGACAAAAAAAACATATG+TGG - chr2.4:15384563-15384582 None:intergenic 25.0%
! CTTATGTATTTACAAAGAGA+GGG + chr2.4:15384254-15384273 MS.gene94585:intron 25.0%
! TATAACACGAGATAAAAGAA+AGG + chr2.4:15382308-15382327 MS.gene94585:intron 25.0%
! TATAATAGAATTGCATTTGC+AGG - chr2.4:15384094-15384113 None:intergenic 25.0%
! TTATGTATTTACAAAGAGAG+GGG + chr2.4:15384255-15384274 MS.gene94585:intron 25.0%
! TTTCAATAATCCAGAGTTTA+GGG - chr2.4:15382171-15382190 None:intergenic 25.0%
!! ACAATATTCTTTTATTAGCC+TGG + chr2.4:15383490-15383509 MS.gene94585:intron 25.0%
!! ATGCATTTTTATAATTGCTG+TGG - chr2.4:15384145-15384164 None:intergenic 25.0%
!! TTCATATTCTTTTACTAGCT+TGG + chr2.4:15383437-15383456 MS.gene94585:intron 25.0%
!! TTTTCAATAATCCAGAGTTT+AGG - chr2.4:15382172-15382191 None:intergenic 25.0%
AAAGTCCTACGTCAAATTTA+GGG - chr2.4:15382650-15382669 None:intergenic 30.0%
AAATTGAGCAAAGTAAATCC+AGG - chr2.4:15383511-15383530 None:intergenic 30.0%
AACCAACAAGATATATACAC+AGG - chr2.4:15383079-15383098 None:intergenic 30.0%
AATGGATCAAAACCTAAAAC+TGG - chr2.4:15384325-15384344 None:intergenic 30.0%
AATGTCCACCAATTATCAAA+GGG - chr2.4:15383792-15383811 None:intergenic 30.0%
ACCAACAAGATATATACACA+GGG - chr2.4:15383078-15383097 None:intergenic 30.0%
AGGTATGGCTCTTATATAAA+AGG - chr2.4:15384388-15384407 None:intergenic 30.0%
CCAAAAATGTCTTCATAGTT+TGG + chr2.4:15383720-15383739 MS.gene94585:intron 30.0%
CTTAATGTGGATATAACATG+TGG + chr2.4:15383461-15383480 MS.gene94585:intron 30.0%
GAATATCGTATGAAAGTTCA+TGG + chr2.4:15383533-15383552 MS.gene94585:intron 30.0%
GGTATGGCTCTTATATAAAA+GGG - chr2.4:15384387-15384406 None:intergenic 30.0%
TAATGTCCACCAATTATCAA+AGG - chr2.4:15383793-15383812 None:intergenic 30.0%
TGGAATATCATAAGACTGTT+TGG + chr2.4:15383374-15383393 MS.gene94585:intron 30.0%
TGTTGTTAAAGGGAATACAT+GGG - chr2.4:15383958-15383977 None:intergenic 30.0%
TTCCACTAAAAATTCTAGTC+AGG - chr2.4:15384356-15384375 None:intergenic 30.0%
TTGAATGTAATCACATACCA+CGG + chr2.4:15381876-15381895 MS.gene94585:intron 30.0%
TTTCTTCATGGTTTCGATAT+CGG - chr2.4:15381404-15381423 None:intergenic 30.0%
! AAACAACATTTTGCAACTCT+TGG + chr2.4:15383001-15383020 MS.gene94585:intron 30.0%
! AACAACATTTTGCAACTCTT+GGG + chr2.4:15383002-15383021 MS.gene94585:intron 30.0%
! AATATGATGTGTTTAAGGCA+CGG + chr2.4:15381737-15381756 MS.gene94585:intron 30.0%
! ATAAAGTTTTACTTCCACCA+AGG - chr2.4:15383303-15383322 None:intergenic 30.0%
! CCAAACTATGAAGACATTTT+TGG - chr2.4:15383723-15383742 None:intergenic 30.0%
! GGATTTACTTTGCTCAATTT+TGG + chr2.4:15383511-15383530 MS.gene94585:intron 30.0%
! TATTACTAGGTGCTAACTTT+TGG + chr2.4:15383347-15383366 MS.gene94585:intron 30.0%
! TCTGAATCAGTGTTGTTAAA+GGG - chr2.4:15383968-15383987 None:intergenic 30.0%
! TTCTAAGTGTTGTCAAATAG+TGG + chr2.4:15383215-15383234 MS.gene94585:intron 30.0%
! TTCTTGGTGATAATCTTTCA+TGG + chr2.4:15381685-15381704 MS.gene94585:intron 30.0%
!! ATAAGCATTCAAGTTGAAAG+AGG - chr2.4:15384408-15384427 None:intergenic 30.0%
!! ATCCTGACTAGAATTTTTAG+TGG + chr2.4:15384351-15384370 MS.gene94585:intron 30.0%
AAGCACTTGTATGACTATGA+AGG - chr2.4:15382588-15382607 None:intergenic 35.0%
ATAGCTTTCTTGAGTTCAGT+TGG - chr2.4:15384469-15384488 None:intergenic 35.0%
ATGTCCACCAATTATCAAAG+GGG - chr2.4:15383791-15383810 None:intergenic 35.0%
ATTATGAAGAGGTAGTAGTG+TGG + chr2.4:15384115-15384134 MS.gene94585:intron 35.0%
ATTCCAAATCAGCAGATCAT+TGG + chr2.4:15384683-15384702 MS.gene94585:CDS 35.0%
CAAAGTCCTACGTCAAATTT+AGG - chr2.4:15382651-15382670 None:intergenic 35.0%
CATATTCTTTATCTGGGCTT+GGG + chr2.4:15383596-15383615 MS.gene94585:intron 35.0%
CTAAATTTGACGTAGGACTT+TGG + chr2.4:15382649-15382668 MS.gene94585:intron 35.0%
GAACGAATTTCGAAATCCTA+GGG - chr2.4:15381487-15381506 None:intergenic 35.0%
GAATGCTTATACCTAGTAAC+TGG + chr2.4:15384418-15384437 MS.gene94585:intron 35.0%
GTTGTTAAAGGGAATACATG+GGG - chr2.4:15383957-15383976 None:intergenic 35.0%
TAGCTTTCTTGAGTTCAGTT+GGG - chr2.4:15384468-15384487 None:intergenic 35.0%
TCATATTCTTTATCTGGGCT+TGG + chr2.4:15383595-15383614 MS.gene94585:intron 35.0%
TCCCTGTGTATATATCTTGT+TGG + chr2.4:15383074-15383093 MS.gene94585:intron 35.0%
TCTAGTCAGGATAGAGAAAA+TGG - chr2.4:15384343-15384362 None:intergenic 35.0%
TGTATGTATTCCTAATGTCC+TGG + chr2.4:15383047-15383066 MS.gene94585:intron 35.0%
TTGAATGTAACCACATGTGT+TGG + chr2.4:15382202-15382221 MS.gene94585:intron 35.0%
! AAGCACTAGTCTGACTATTA+AGG - chr2.4:15382109-15382128 None:intergenic 35.0%
! AATTTAGGGCATGTTTGGAT+TGG - chr2.4:15382636-15382655 None:intergenic 35.0%
! ACAACATTTTGCAACTCTTG+GGG + chr2.4:15383003-15383022 MS.gene94585:intron 35.0%
! AGGTTAGTGCTGTTAAATAG+CGG + chr2.4:15383120-15383139 MS.gene94585:intron 35.0%
! CTACTATTTTCTGCAATCCT+TGG + chr2.4:15383283-15383302 MS.gene94585:intron 35.0%
! CTCTGAATCAGTGTTGTTAA+AGG - chr2.4:15383969-15383988 None:intergenic 35.0%
! GTGTTGTTAAAGGGAATACA+TGG - chr2.4:15383959-15383978 None:intergenic 35.0%
! TCTGCTGTTATACCAGTTTT+AGG + chr2.4:15384310-15384329 MS.gene94585:intron 35.0%
! TTACTAGCTTGGACTTAATG+TGG + chr2.4:15383448-15383467 MS.gene94585:intron 35.0%
! TTCTAAGTATCGTTAACCCT+AGG + chr2.4:15381468-15381487 MS.gene94585:intron 35.0%
!! AATGATCTGCTGATTTGGAA+TGG - chr2.4:15384684-15384703 None:intergenic 35.0%
!! CATTCAAGTTGAAAGAGGTA+TGG - chr2.4:15384403-15384422 None:intergenic 35.0%
!! GTTGTTTTCGTAAGCAATCT+TGG + chr2.4:15382498-15382517 MS.gene94585:intron 35.0%
!!! AGGTGCTAACTTTTGGAATT+TGG + chr2.4:15383354-15383373 MS.gene94585:intron 35.0%
!!! GCTGTTTTTGTAAGCTATCT+TGG + chr2.4:15382362-15382381 MS.gene94585:intron 35.0%
!!! TGTTGTCGTTTTGTGATACA+TGG + chr2.4:15383187-15383206 MS.gene94585:intron 35.0%
AAAGGGAATACATGGGGAAA+TGG - chr2.4:15383951-15383970 None:intergenic 40.0%
ACATATGTGGTGAGTGAAGT+CGG - chr2.4:15384550-15384569 None:intergenic 40.0%
ACATGCCCTAAATTTGACGT+AGG + chr2.4:15382642-15382661 MS.gene94585:intron 40.0%
AGAAAGCTATAATGCAAGCC+AGG + chr2.4:15384479-15384498 MS.gene94585:CDS 40.0%
AGAAGCTCAACGTTTCTTCA+TGG - chr2.4:15381416-15381435 None:intergenic 40.0%
ATGCCTTCGATCATAAGTGT+TGG + chr2.4:15382260-15382279 MS.gene94585:intron 40.0%
ATTTAACAGCACTAACCTCC+AGG - chr2.4:15383118-15383137 None:intergenic 40.0%
GACGTAGGACTTTGGATTAT+TGG + chr2.4:15382657-15382676 MS.gene94585:intron 40.0%
GCACTTCATTGAACACTAAG+AGG + chr2.4:15382899-15382918 MS.gene94585:CDS 40.0%
GCCATAGCACTAAAGTATAG+CGG + chr2.4:15383152-15383171 MS.gene94585:intron 40.0%
GGAACGAATTTCGAAATCCT+AGG - chr2.4:15381488-15381507 None:intergenic 40.0%
GTCTAGATTAAGAGCAGTGT+TGG + chr2.4:15383813-15383832 MS.gene94585:intron 40.0%
GTGTCAGTGCTAGAAATTGT+CGG + chr2.4:15382228-15382247 MS.gene94585:intron 40.0%
TATATACACAGGGAAACACC+AGG - chr2.4:15383068-15383087 None:intergenic 40.0%
TCCGCTATACTTTAGTGCTA+TGG - chr2.4:15383156-15383175 None:intergenic 40.0%
TGTCCACCAATTATCAAAGG+GGG - chr2.4:15383790-15383809 None:intergenic 40.0%
! AACATTTTGCAACTCTTGGG+GGG + chr2.4:15383005-15383024 MS.gene94585:intron 40.0%
! AGTTTAGGGCATGATTGGAT+TGG - chr2.4:15382157-15382176 None:intergenic 40.0%
! ATACCCCCTTTGATAATTGG+TGG + chr2.4:15383784-15383803 MS.gene94585:intron 40.0%
! CAACATTTTGCAACTCTTGG+GGG + chr2.4:15383004-15383023 MS.gene94585:intron 40.0%
! CCAATACCCCCTTTGATAAT+TGG + chr2.4:15383781-15383800 MS.gene94585:intron 40.0%
! CCAATTATCAAAGGGGGTAT+TGG - chr2.4:15383784-15383803 None:intergenic 40.0%
! CGTCAAATTTAGGGCATGTT+TGG - chr2.4:15382641-15382660 None:intergenic 40.0%
! CTATTTTCTGCAATCCTTGG+TGG + chr2.4:15383286-15383305 MS.gene94585:intron 40.0%
! TTATGATCGAAGGCATGTCT+GGG - chr2.4:15382256-15382275 None:intergenic 40.0%
! TTGCCAATGATCTGCTGATT+TGG - chr2.4:15384689-15384708 None:intergenic 40.0%
!! ATTAAGAGCAGTGTTGGCAA+TGG + chr2.4:15383819-15383838 MS.gene94585:intron 40.0%
!! TGGGGGGTTTAACTTTTACA+AGG + chr2.4:15383021-15383040 MS.gene94585:intron 40.0%
!!! GCAAATTTTTTGACAGCCTC+CGG - chr2.4:15383924-15383943 None:intergenic 40.0%
ACTTGTTTGCGCAGATGATC+GGG + chr2.4:15384441-15384460 MS.gene94585:intron 45.0%
AGCTATAATGCAAGCCAGGA+TGG + chr2.4:15384483-15384502 MS.gene94585:CDS 45.0%
AGGACCCGTTTCACAAGATT+GGG + chr2.4:15381294-15381313 MS.gene94585:CDS 45.0%
AGTAAGCTTCTTGTCCATCC+TGG - chr2.4:15384500-15384519 None:intergenic 45.0%
ATTCCTTCTCAAATTGCCGG+AGG + chr2.4:15383905-15383924 MS.gene94585:intron 45.0%
CGCAAACAAGTCCAGTTACT+AGG - chr2.4:15384432-15384451 None:intergenic 45.0%
GAACACTAAGAGGCTTGATG+AGG + chr2.4:15382909-15382928 MS.gene94585:CDS 45.0%
GATGGACAAGAAGCTTACTC+AGG + chr2.4:15384501-15384520 MS.gene94585:CDS 45.0%
GCACCAACACTTATGATCGA+AGG - chr2.4:15382266-15382285 None:intergenic 45.0%
GCATTTGCAGGCAACAAGAT+AGG - chr2.4:15384082-15384101 None:intergenic 45.0%
GGCATTCCTTCTCAAATTGC+CGG + chr2.4:15383902-15383921 MS.gene94585:intron 45.0%
TCAGCACTCTGACCAGAAAA+TGG - chr2.4:15382818-15382837 None:intergenic 45.0%
TCAGCAGATCATTGGCAAGT+TGG + chr2.4:15384691-15384710 MS.gene94585:CDS 45.0%
TCATTTCTTCCCACGCAGTT+TGG - chr2.4:15384732-15384751 None:intergenic 45.0%
TCCAATCATGCCCTAAACTC+TGG + chr2.4:15382158-15382177 MS.gene94585:intron 45.0%
! ATGCCTTCGATCAGAAGTGT+TGG + chr2.4:15382402-15382421 MS.gene94585:intron 45.0%
! CTTATGATCGAAGGCATGTC+TGG - chr2.4:15382257-15382276 None:intergenic 45.0%
! GAATTTAGCTCGTGAGTCTC+CGG + chr2.4:15382939-15382958 MS.gene94585:intron 45.0%
! GACTTGTTTGCGCAGATGAT+CGG + chr2.4:15384440-15384459 MS.gene94585:intron 45.0%
! GATGTGTTTAAGGCACGGTA+TGG + chr2.4:15381742-15381761 MS.gene94585:intron 45.0%
! TATGATCGAAGGCATGTCTG+GGG - chr2.4:15382255-15382274 None:intergenic 45.0%
! TCCAGAGTTTAGGGCATGAT+TGG - chr2.4:15382162-15382181 None:intergenic 45.0%
!! GTCAGAGTGCTGATTGTGTT+GGG + chr2.4:15382825-15382844 MS.gene94585:intron 45.0%
!! GTGTTCATATGGCGGAGTTT+TGG - chr2.4:15383852-15383871 None:intergenic 45.0%
!!! CGTTGATTCGCTCCATTTTC+TGG + chr2.4:15382803-15382822 MS.gene94585:intron 45.0%
ATCTGGGCTTGGGATTACTG+TGG + chr2.4:15383606-15383625 MS.gene94585:intron 50.0%
CAGGACCCGTTTCACAAGAT+TGG + chr2.4:15381293-15381312 MS.gene94585:CDS 50.0%
CAGGGAAACACCAGGACATT+AGG - chr2.4:15383060-15383079 None:intergenic 50.0%
CATGGTTTCGATATCGGCAC+CGG - chr2.4:15381398-15381417 None:intergenic 50.0%
CTGACACACACCAACACATG+TGG - chr2.4:15382215-15382234 None:intergenic 50.0%
GCTCCAACACTTCTGATCGA+AGG - chr2.4:15382408-15382427 None:intergenic 50.0%
GGCTCCCAATCTTGTGAAAC+GGG - chr2.4:15381301-15381320 None:intergenic 50.0%
GGGCTCCCAATCTTGTGAAA+CGG - chr2.4:15381302-15381321 None:intergenic 50.0%
TCGTACTCCTGGATCACTTG+AGG - chr2.4:15384650-15384669 None:intergenic 50.0%
TGAGAAGCCTCAAGTGATCC+AGG + chr2.4:15384640-15384659 MS.gene94585:CDS 50.0%
TTGAGAAGGAATGCCCACCA+TGG - chr2.4:15383897-15383916 None:intergenic 50.0%
TTTGTCATCCTTCCTGGCAG+CGG - chr2.4:15381353-15381372 None:intergenic 50.0%
! ACGCCTTCGATCAGAAGTGT+CGG + chr2.4:15381929-15381948 MS.gene94585:intron 50.0%
! GACAGCTTTGTCATCCTTCC+TGG - chr2.4:15381359-15381378 None:intergenic 50.0%
! TGATCCAGGAGTACGAGTCA+GGG + chr2.4:15384654-15384673 MS.gene94585:CDS 50.0%
! TTGAAGCATGCGAGTTGACC+TGG + chr2.4:15383097-15383116 MS.gene94585:intron 50.0%
!! AAGAGCAGTGTTGGCAATGG+TGG + chr2.4:15383822-15383841 MS.gene94585:intron 50.0%
!! GGTCAGAGTGCTGATTGTGT+TGG + chr2.4:15382824-15382843 MS.gene94585:intron 50.0%
AGCTTTCTTGCGGAGGACGA+CGG - chr2.4:15381323-15381342 None:intergenic 55.0%
CAGCCTCCGGCAATTTGAGA+AGG - chr2.4:15383911-15383930 None:intergenic 55.0%
GCCATATGAACACGCCATGG+CGG + chr2.4:15383859-15383878 MS.gene94585:intron 55.0%
GCCATGGCGTGTTCATATGG+CGG - chr2.4:15383860-15383879 None:intergenic 55.0%
TCCGCCATATGAACACGCCA+TGG + chr2.4:15383856-15383875 MS.gene94585:intron 55.0%
! AAGCATGCGAGTTGACCTGG+AGG + chr2.4:15383100-15383119 MS.gene94585:intron 55.0%
! CTTCTGATCGAAGGCGTGTC+TGG - chr2.4:15381926-15381945 None:intergenic 55.0%
! CTTTCCCTGACTCGTACTCC+TGG - chr2.4:15384661-15384680 None:intergenic 55.0%
! GCACCGACACTTCTGATCGA+AGG - chr2.4:15381935-15381954 None:intergenic 55.0%
! GGCAAGTTGGAGAGAGCTCT+TGG + chr2.4:15384704-15384723 MS.gene94585:CDS 55.0%
! GTGATCCAGGAGTACGAGTC+AGG + chr2.4:15384653-15384672 MS.gene94585:CDS 55.0%
! TTTTGCAGCATCACACGCCG+TGG - chr2.4:15381896-15381915 None:intergenic 55.0%
!! CTCTTGGTGCCAAACTGCGT+GGG + chr2.4:15384720-15384739 MS.gene94585:CDS 55.0%
!! GCAGTGTTGGCAATGGTGGA+AGG + chr2.4:15383826-15383845 MS.gene94585:intron 55.0%
GCCGCCATGGCGTGTTCATA+TGG - chr2.4:15383863-15383882 None:intergenic 60.0%
GCTTTCTTGCGGAGGACGAC+GGG - chr2.4:15381322-15381341 None:intergenic 60.0%
!! GCCTATGGTGCTGCCATGGT+GGG + chr2.4:15383881-15383900 MS.gene94585:intron 60.0%
!! GCTCTTGGTGCCAAACTGCG+TGG + chr2.4:15384719-15384738 MS.gene94585:CDS 60.0%
CGGCGGTGGGAGCTTTCTTG+CGG - chr2.4:15381333-15381352 None:intergenic 65.0%
CGGTGGGAGCTTTCTTGCGG+AGG - chr2.4:15381330-15381349 None:intergenic 65.0%
GAACACGCCATGGCGGCCTA+TGG + chr2.4:15383866-15383885 MS.gene94585:intron 65.0%
GCCCACCATGGCAGCACCAT+AGG - chr2.4:15383885-15383904 None:intergenic 65.0%
GTCATCCTTCCTGGCAGCGG+CGG - chr2.4:15381350-15381369 None:intergenic 65.0%
!! GGCCTATGGTGCTGCCATGG+TGG + chr2.4:15383880-15383899 MS.gene94585:intron 65.0%
ATCCTTCCTGGCAGCGGCGG+TGG - chr2.4:15381347-15381366 None:intergenic 70.0%
GGCAGCACCATAGGCCGCCA+TGG - chr2.4:15383876-15383895 None:intergenic 70.0%
GTCAACGCCGCTCGTCGTGC+CGG + chr2.4:15381376-15381395 MS.gene94585:CDS 70.0%
TCCTTCCTGGCAGCGGCGGT+GGG - chr2.4:15381346-15381365 None:intergenic 70.0%
!! ATCGGCACCGGCACGACGAG+CGG - chr2.4:15381386-15381405 None:intergenic 70.0%
!! GGCGGCCTATGGTGCTGCCA+TGG + chr2.4:15383877-15383896 MS.gene94585:intron 70.0%
AAGCTCCCACCGCCGCTGCC+AGG + chr2.4:15381338-15381357 MS.gene94585:CDS 75.0%
TCCCACCGCCGCTGCCAGGA+AGG + chr2.4:15381342-15381361 MS.gene94585:CDS 75.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 15381289 15384751 15381289 ID=MS.gene94585
chr2.4 mRNA 15381289 15384751 15381289 ID=MS.gene94585.t1;Parent=MS.gene94585
chr2.4 exon 15381289 15381424 15381289 ID=MS.gene94585.t1.exon1;Parent=MS.gene94585.t1
chr2.4 CDS 15381289 15381424 15381289 ID=cds.MS.gene94585.t1;Parent=MS.gene94585.t1
chr2.4 exon 15382866 15382949 15382866 ID=MS.gene94585.t1.exon2;Parent=MS.gene94585.t1
chr2.4 CDS 15382866 15382949 15382866 ID=cds.MS.gene94585.t1;Parent=MS.gene94585.t1
chr2.4 exon 15384455 15384537 15384455 ID=MS.gene94585.t1.exon3;Parent=MS.gene94585.t1
chr2.4 CDS 15384455 15384537 15384455 ID=cds.MS.gene94585.t1;Parent=MS.gene94585.t1
chr2.4 exon 15384632 15384751 15384632 ID=MS.gene94585.t1.exon4;Parent=MS.gene94585.t1
chr2.4 CDS 15384632 15384751 15384632 ID=cds.MS.gene94585.t1;Parent=MS.gene94585.t1
Gene Sequence

>MS.gene94585

ATGGCAGGACCCGTTTCACAAGATTGGGAGCCCGTCGTCCTCCGCAAGAAAGCTCCCACCGCCGCTGCCAGGAAGGATGACAAAGCTGTCAACGCCGCTCGTCGTGCCGGTGCCGATATCGAAACCATGAAGAAACGTTGAGCTTCTTTCCTTTTCTTTCATTTCTTTAATCATTCGTTTTCTAAGTATCGTTAACCCTAGGATTTCGAAATTCGTTCCTTCGTTTATTAAAAATAAATTTCATGTTATCTTTGTGCTTTCGATCAAAGCGTTTAATTTGTTAAATCACTGTTTGGATTGTTGATATTTCTTATTAGGGTTTGTGTTGTTAATTTTTTTTATATTGCATTTTTTTATTTGTTATATCTATGTTAAATTTGAAATAATAAATGTTTTTTCTTGGTGATAATCTTTCATGGTTTTTCATTTCTGTTGTTGATTTTCTATTAATATGATGTGTTTAAGGCACGGTATGGATAAACAGCTTAATTTACAGCTTATAGCACAAACATGTCTCATGATAAGTGCTTATGTTTAAACAACGACAAGTGACACCGACAATAATTTGAAAAAAAAAAAAGTGATAATTGAATGTAATCACATACCACGGCGTGTGATGCTGCAAAAACTGACACCAGACACGCCTTCGATCAGAAGTGTCGGTGCTATGTAGTGTGTAAGCTATTTCTATAACACGAGATAAAATAAAGTCACATTGTTTTCGTATAAGCTATGTTTTCATATGTTATCTTGGAGAGCTTATGAAAATAAGCTGAAACAACTTATGAACAAGTCATCAGCTGTTTTTATAAGCTCTCCTTAATAGTCAGACTAGTGCTTATGCTATTATATAAGCTCAAATAAGCCAATCCAATCATGCCCTAAACTCTGGATTATTGAAAAAAAATGAGAATTGAATGTAACCACATGTGTTGGTGTGTGTCAGTGCTAGAAATTGTCGGACCCCAGACATGCCTTCGATCATAAGTGTTGGTGCTACATATTGTATAAGCTAGTTCTATAACACGAGATAAAAGAAAGGCACATTTTTTTTTTCTTCGTATAAGCTATTAGCTGTTTTTGTAAGCTATCTTGGAGAGTTTATGAAAATAAATGCCTTCGATCAGAAGTGTTGGAGCTACATAGTGTATAAGCTATTTCTATAACACGAGATAAATTAAATTCACATTGATTTCGTATAATCTATGAGTTGTTTTCGTAAGCAATCTTGGATAGCTTATGAAAATAAGCTGAAAACAACTCATGAACAAGTCATCAGCTGTTTTCATAAGTTCTCCTTCATAGTCATACAAGTGCTTATGCTATTATATAAGCTCAAATAAGCCAATCCAAACATGCCCTAAATTTGACGTAGGACTTTGGATTATTGGATTTTCAAGACTCTAACACAAATAGTTTATGAGCAGTTTTCTGCAAGTTGTTTTTGTAATTATGAGCAGTTTTTTTCATTGATTGATCTTTTACTTGTATAATTGAATGTTTCTCATTTTTATCGTTGATTCGCTCCATTTTCTGGTCAGAGTGCTGATTGTGTTGGGTTGTTCTATACATTACAGATAATGCTGCGACAAACAAAGCTGCTTCTAGCAGCACTTCATTGAACACTAAGAGGCTTGATGAGGATACAGAGAATTTAGCTCGTGAGTCTCCGGTTACTCTATTATTTTTTTCCTTCCCATTTTATAATATTGAAACAACATTTTGCAACTCTTGGGGGGTTTAACTTTTACAAGGTATTGTATGTATTCCTAATGTCCTGGTGTTTCCCTGTGTATATATCTTGTTGGTTGAAGCATGCGAGTTGACCTGGAGGTTAGTGCTGTTAAATAGCGGTATAGTAGTGCCATAGCACTAAAGTATAGCGGAATTTAACTTACTGTTGTCGTTTTGTGATACATGGTTTAGTTCTAAGTGTTGTCAAATAGTGGCTATAGCTGTGACATAACATTGAAGCATAACATAATTTGAACAAACTACTATTTTCTGCAATCCTTGGTGGAAGTAAAACTTTATCACCATAGAAGTTATCTATAGATGTATTACTAGGTGCTAACTTTTGGAATTTGGAATATCATAAGACTGTTTGGAAGTGCTATATGTATATATCTTCCATAATTAATTGTGGAATTCATATTCTTTTACTAGCTTGGACTTAATGTGGATATAACATGTGGTTTTTTACAATATTCTTTTATTAGCCTGGATTTACTTTGCTCAATTTTGGAATATCGTATGAAAGTTCATGGTATTATTATCTGCAATTATTACATAATTACTGAGAAAATTCATATTCTTTATCTGGGCTTGGGATTACTGTGGACATGTGTACTCTTTGTTGTTTGATTTCATATCTCAGATTTTTGTTATATTAATATTATTTATGTTTACAATAATTCTTTTTATCACAATGCCAAAAATGTCTTCATAGTTTGGACATGAGAATGTGTGACTGTACTGTGTTCTAGTGTAGTCCAATACCCCCTTTGATAATTGGTGGACATTAGTCTAGATTAAGAGCAGTGTTGGCAATGGTGGAAGGCCAAAACTCCGCCATATGAACACGCCATGGCGGCCTATGGTGCTGCCATGGTGGGCATTCCTTCTCAAATTGCCGGAGGCTGTCAAAAAATTTGCAATGCCATTTCCCCATGTATTCCCTTTAACAACACTGATTCAGAGATCAGAGCTCTCGAGACTGATTCAGATCTGATTTAGAGCTCTATAATAGTAATCCCCATCCTAATCAAATATTTTTCTACAAATTGATATCCTATCTTGTTGCCTGCAAATGCAATTCTATTATAAATTATGAAGAGGTAGTAGTGTGGTCTACCACAGCAATTATAAAAATGCATCTCAGTTTACAATGTAGAATCTGTTGCTTGTGCTTTATTTCTGTTTGACGAATGACAAAATAGAAATTTGTAGTGTTCAGCAATTCAAACTTATGTATTTACAAAGAGAGGGGCTTTGTTTAACAGAGCAGTGTAGCATGCTAGCTCTGCTGTTATACCAGTTTTAGGTTTTGATCCATTTTCTCTATCCTGACTAGAATTTTTAGTGGAACATCAACTCCCTTTTATATAAGAGCCATACCTCTTTCAACTTGAATGCTTATACCTAGTAACTGGACTTGTTTGCGCAGATGATCGGGTCCCAACTGAACTCAAGAAAGCTATAATGCAAGCCAGGATGGACAAGAAGCTTACTCAGGCACAACTTGCTCAAGTATTGCTGCCGACTTCACTCACCACATATGTTTTTTTTGTCTTGATTTCATTATTTGAAGTTTGATATTAATAACGAGCATGTTTGATTGCAGATTATCAATGAGAAGCCTCAAGTGATCCAGGAGTACGAGTCAGGGAAAGCCATTCCAAATCAGCAGATCATTGGCAAGTTGGAGAGAGCTCTTGGTGCCAAACTGCGTGGGAAGAAATGA
Protein sequence

>MS.gene94585.t1

MAGPVSQDWEPVVLRKKAPTAAARKDDKAVNAARRAGADIETMKKHNAATNKAASSSTSLNTKRLDEDTENLAHDRVPTELKKAIMQARMDKKLTQAQLAQIINEKPQVIQEYESGKAIPNQQIIGKLERALGAKLRGKK