AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35285

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35285.t1 MTR_2g084220 98.571 140 2 0 1 140 1 140 1.19e-97 277
MS.gene35285.t1 MTR_4g080090 92.754 138 10 0 3 140 5 142 2.66e-84 243
MS.gene35285.t1 MTR_6g018330 75.182 137 18 1 4 140 23 143 9.05e-64 191
MS.gene35285.t1 MTR_6g086280 52.174 138 66 0 2 139 9 146 1.48e-45 145
MS.gene35285.t1 MTR_6g086280 52.174 138 66 0 2 139 9 146 1.59e-45 145
MS.gene35285.t1 MTR_1g047000 92.683 41 3 0 100 140 16 56 5.88e-21 80.5
MS.gene35285.t1 MTR_7g117675 95.000 40 2 0 90 129 1 40 2.44e-20 79.3
MS.gene35285.t1 MTR_4g103930 55.844 77 21 1 30 93 45 121 6.40e-18 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35285.t1 AT3G58680 81.159 138 26 0 3 140 5 142 7.25e-82 237
MS.gene35285.t1 AT2G42680 82.609 138 24 0 3 140 5 142 1.99e-81 236
MS.gene35285.t1 AT3G24500 51.799 139 65 1 3 139 7 145 2.32e-44 142
MS.gene35285.t1 AT3G24500 44.944 89 47 1 3 89 7 95 3.41e-17 72.0

Find 45 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCCAATGATCTGCTGATT+TGG 0.246748 2.1:-17489299 None:intergenic
TTTCTTCATGGTTTCGATAT+CGG 0.259177 2.1:-17486012 None:intergenic
ATCCTTCCTGGCAGCGGCGG+TGG 0.288753 2.1:-17485955 None:intergenic
GACAGCTTTGTCATCCTTCC+TGG 0.316434 2.1:-17485967 None:intergenic
CAGGACCCGTTTCACAAGAT+TGG 0.366530 2.1:+17485904 MS.gene35285:CDS
GACTTGTTTGCGCAGATGAT+CGG 0.384244 2.1:+17489054 MS.gene35285:intron
GTGATCCAGGAGTACGAGTC+AGG 0.396136 2.1:+17489266 MS.gene35285:CDS
TCCTTCCTGGCAGCGGCGGT+GGG 0.408929 2.1:-17485954 None:intergenic
AGAAGCTCAACGTTTCTTCA+TGG 0.430879 2.1:-17486024 None:intergenic
TCATTTCTTCCCACGCAGTT+TGG 0.431740 2.1:-17489342 None:intergenic
GCACTTCATTGAACACTAAG+AGG 0.438741 2.1:+17487515 MS.gene35285:CDS
AGGACCCGTTTCACAAGATT+GGG 0.440017 2.1:+17485905 MS.gene35285:CDS
GGCAAGTTGGAGAGAGCTCT+TGG 0.442294 2.1:+17489317 MS.gene35285:CDS
AAGCTCCCACCGCCGCTGCC+AGG 0.442991 2.1:+17485949 MS.gene35285:CDS
GTCAACGCCGCTCGTCGTGC+CGG 0.452741 2.1:+17485987 MS.gene35285:CDS
AGTAAGCTTCTTGTCCATCC+TGG 0.456590 2.1:-17489111 None:intergenic
ACTTGTTTGCGCAGATGATC+GGG 0.459972 2.1:+17489055 MS.gene35285:intron
CTTTCCCTGACTCGTACTCC+TGG 0.461120 2.1:-17489271 None:intergenic
ATTCCAAATCAGCAGATCAT+TGG 0.464427 2.1:+17489296 MS.gene35285:CDS
GGTTCCCAATCTTGTGAAAC+GGG 0.465547 2.1:-17485909 None:intergenic
GCTTTCTTGCGGAGGACGAC+GGG 0.478528 2.1:-17485930 None:intergenic
ATAGCTTTCTTGAGTTCAGT+TGG 0.482895 2.1:-17489080 None:intergenic
TAGCTTTCTTGAGTTCAGTT+GGG 0.492776 2.1:-17489079 None:intergenic
TGAGAAGCCTCAAGTGATCC+AGG 0.497642 2.1:+17489253 MS.gene35285:CDS
TCAGCAGATCATTGGCAAGT+TGG 0.510535 2.1:+17489304 MS.gene35285:CDS
GGGTTCCCAATCTTGTGAAA+CGG 0.516848 2.1:-17485910 None:intergenic
TCCCACCGCCGCTGCCAGGA+AGG 0.517927 2.1:+17485953 MS.gene35285:CDS
TCAAATCATTCAACCATGGC+AGG 0.533957 2.1:+17485885 None:intergenic
TTTGTCATCCTTCCTGGCAG+CGG 0.537196 2.1:-17485961 None:intergenic
AGAAAGCTATAATGCAAGCC+AGG 0.541759 2.1:+17489093 MS.gene35285:CDS
AGCTTTCTTGCGGAGGACGA+CGG 0.546703 2.1:-17485931 None:intergenic
AATGATCTGCTGATTTGGAA+TGG 0.551980 2.1:-17489294 None:intergenic
AGCTATAATGCAAGCCAGGA+TGG 0.555000 2.1:+17489097 MS.gene35285:CDS
CGGCGGTGGGAGCTTTCTTG+CGG 0.555292 2.1:-17485941 None:intergenic
CATGGTTTCGATATCGGCAC+CGG 0.557535 2.1:-17486006 None:intergenic
CTCTTGGTGCCAAACTGCGT+GGG 0.558179 2.1:+17489333 MS.gene35285:CDS
GTCATCCTTCCTGGCAGCGG+CGG 0.565962 2.1:-17485958 None:intergenic
CGGTGGGAGCTTTCTTGCGG+AGG 0.580235 2.1:-17485938 None:intergenic
TTGTGAAACGGGTCCTGCCA+TGG 0.588651 2.1:-17485898 None:intergenic
ATCGGCACCGGCACGACGAG+CGG 0.589916 2.1:-17485994 None:intergenic
GCTCTTGGTGCCAAACTGCG+TGG 0.608518 2.1:+17489332 MS.gene35285:CDS
TGATCCAGGAGTACGAGTCA+GGG 0.620671 2.1:+17489267 MS.gene35285:CDS
TCGTACTCCTGGATCACTTG+AGG 0.629262 2.1:-17489260 None:intergenic
GAACACTAAGAGGCTTGATG+AGG 0.646364 2.1:+17487525 MS.gene35285:CDS
GATGGACAAGAAGCTTACTC+AGG 0.656852 2.1:+17489115 MS.gene35285:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGTTTCAATATTATAAAAT+GGG - chr2.1:17487603-17487622 None:intergenic 10.0%
!!! AAATAATAAATGTTTTTTCT+TGG + chr2.1:17486279-17486298 MS.gene35285:intron 10.0%
!!! AAATTTATTTTAATAAACGA+AGG - chr2.1:17486120-17486139 None:intergenic 10.0%
!!! TTTTTTTTTCAAATTATTGT+CGG - chr2.1:17486458-17486477 None:intergenic 10.0%
!! AAAAAAAATAATAGAGTAAC+CGG - chr2.1:17487577-17487596 None:intergenic 15.0%
!! AAAATTCATATTCTTTATCT+GGG + chr2.1:17488206-17488225 MS.gene35285:intron 15.0%
!! ATTGTTGATATTTCTTATTA+GGG + chr2.1:17486195-17486214 MS.gene35285:intron 15.0%
!! TTCTATTATAAATTATGAAG+AGG + chr2.1:17488718-17488737 MS.gene35285:intron 15.0%
!! TTGTAGAAAAATATTTGATT+AGG - chr2.1:17488665-17488684 None:intergenic 15.0%
!!! GTTGTTTCAATATTATAAAA+TGG - chr2.1:17487604-17487623 None:intergenic 15.0%
!! AAAAATATTTGATTAGGATG+GGG - chr2.1:17488659-17488678 None:intergenic 20.0%
!! AGAAAAATATTTGATTAGGA+TGG - chr2.1:17488661-17488680 None:intergenic 20.0%
!! ATATCTTCCATAATTAATTG+TGG + chr2.1:17488028-17488047 MS.gene35285:intron 20.0%
!! ATGAATTCCACAATTAATTA+TGG - chr2.1:17488038-17488057 None:intergenic 20.0%
!! GAAAAATATTTGATTAGGAT+GGG - chr2.1:17488660-17488679 None:intergenic 20.0%
!! GAAAATTCATATTCTTTATC+TGG + chr2.1:17488205-17488224 MS.gene35285:intron 20.0%
!! GATTAAAGAAATGAAAGAAA+AGG - chr2.1:17486052-17486071 None:intergenic 20.0%
!! GATTGTTGATATTTCTTATT+AGG + chr2.1:17486194-17486213 MS.gene35285:intron 20.0%
!!! TAATTTGTTAAATCACTGTT+TGG + chr2.1:17486172-17486191 MS.gene35285:intron 20.0%
!!! TATGTTTTCATATGTTATCT+TGG + chr2.1:17486640-17486659 MS.gene35285:intron 20.0%
! AAAACAGCTAATAGCTTATA+CGG - chr2.1:17486967-17486986 None:intergenic 25.0%
! ACTTATGTATTTACAAAGAG+AGG + chr2.1:17488867-17488886 MS.gene35285:intron 25.0%
! CTATTAATATGATGTGTGTA+AGG + chr2.1:17486342-17486361 MS.gene35285:intron 25.0%
! CTTATGTATTTACAAAGAGA+GGG + chr2.1:17488868-17488887 MS.gene35285:intron 25.0%
! TATAACACGAGATAAAAGAA+AGG + chr2.1:17486928-17486947 MS.gene35285:intron 25.0%
! TATAATAGAATTGCATTTGC+AGG - chr2.1:17488708-17488727 None:intergenic 25.0%
! TCAAGACAAAAAAACATATG+TGG - chr2.1:17489177-17489196 None:intergenic 25.0%
! TTATGTATTTACAAAGAGAG+GGG + chr2.1:17488869-17488888 MS.gene35285:intron 25.0%
! TTTCAATAATCCAGAGTTTA+GGG - chr2.1:17486791-17486810 None:intergenic 25.0%
!! ACAATATTCTTTTATTAGCC+TGG + chr2.1:17488106-17488125 MS.gene35285:intron 25.0%
!! ATGCATTTTTATAATTGCTG+TGG - chr2.1:17488759-17488778 None:intergenic 25.0%
!! TTCATATTCTTTTACTAGCT+TGG + chr2.1:17488053-17488072 MS.gene35285:intron 25.0%
!! TTTTCAATAATCCAGAGTTT+AGG - chr2.1:17486792-17486811 None:intergenic 25.0%
!!! CATTGATTTCGTATAATCTA+TGG + chr2.1:17487092-17487111 MS.gene35285:intron 25.0%
AAAGTCCTACGTCAAATTTA+GGG - chr2.1:17487266-17487285 None:intergenic 30.0%
AAATTGAGCAAAGTAAATCC+AGG - chr2.1:17488127-17488146 None:intergenic 30.0%
AACCAACAAGATATATACAC+AGG - chr2.1:17487695-17487714 None:intergenic 30.0%
AATGGATCAAAACCTAAAAC+TGG - chr2.1:17488939-17488958 None:intergenic 30.0%
AATGTCCACCAATTATCAAA+GGG - chr2.1:17488408-17488427 None:intergenic 30.0%
ACCAACAAGATATATACACA+GGG - chr2.1:17487694-17487713 None:intergenic 30.0%
AGGTATGGCTCTTATATAAA+AGG - chr2.1:17489002-17489021 None:intergenic 30.0%
CCAAAAATGTCTTCATAGTT+TGG + chr2.1:17488336-17488355 MS.gene35285:intron 30.0%
CTTAATGTGGATATAACATG+TGG + chr2.1:17488077-17488096 MS.gene35285:intron 30.0%
GAATATCGTATGAAAGTTCA+TGG + chr2.1:17488149-17488168 MS.gene35285:intron 30.0%
GGTATGGCTCTTATATAAAA+GGG - chr2.1:17489001-17489020 None:intergenic 30.0%
TAATGTCCACCAATTATCAA+AGG - chr2.1:17488409-17488428 None:intergenic 30.0%
TGGAATATCATAAGACTGTT+TGG + chr2.1:17487990-17488009 MS.gene35285:intron 30.0%
TGTTGTTAAAGGGAATACAT+GGG - chr2.1:17488572-17488591 None:intergenic 30.0%
TTACCTATAGGTGTATTACT+AGG + chr2.1:17487950-17487969 MS.gene35285:intron 30.0%
TTCCACTAAAAATTCTAGTC+AGG - chr2.1:17488970-17488989 None:intergenic 30.0%
TTGAATGTAATCACATACGA+CGG + chr2.1:17486496-17486515 MS.gene35285:intron 30.0%
TTTCTTCATGGTTTCGATAT+CGG - chr2.1:17486015-17486034 None:intergenic 30.0%
! AAAAAGTTTTACTTCCACCA+AGG - chr2.1:17487919-17487938 None:intergenic 30.0%
! AAACAACATTTTGCAACTCT+TGG + chr2.1:17487617-17487636 MS.gene35285:intron 30.0%
! AACAACATTTTGCAACTCTT+GGG + chr2.1:17487618-17487637 MS.gene35285:intron 30.0%
! CCAAACTATGAAGACATTTT+TGG - chr2.1:17488339-17488358 None:intergenic 30.0%
! GGATTTACTTTGCTCAATTT+TGG + chr2.1:17488127-17488146 MS.gene35285:intron 30.0%
! TATTACTAGGTGCTAACTTT+TGG + chr2.1:17487963-17487982 MS.gene35285:intron 30.0%
! TCTGAATCAGTGTTGTTAAA+GGG - chr2.1:17488582-17488601 None:intergenic 30.0%
! TTCTAAGTGTTGTCAAATAG+TGG + chr2.1:17487831-17487850 MS.gene35285:intron 30.0%
! TTCTTGGTGATAATCTTTCA+TGG + chr2.1:17486295-17486314 MS.gene35285:intron 30.0%
!! ATAAGCATTCAAGTTGAAAG+AGG - chr2.1:17489022-17489041 None:intergenic 30.0%
!! ATCCTGACTAGAATTTTTAG+TGG + chr2.1:17488965-17488984 MS.gene35285:intron 30.0%
AAGCACTTGTATGACTATGA+AGG - chr2.1:17487204-17487223 None:intergenic 35.0%
AATATGATGTGTGTAAGGCA+CGG + chr2.1:17486347-17486366 MS.gene35285:intron 35.0%
ACACCTATAGGTAACTTCTA+TGG - chr2.1:17487944-17487963 None:intergenic 35.0%
ATAGCTTTCTTGAGTTCAGT+TGG - chr2.1:17489083-17489102 None:intergenic 35.0%
ATGTCCACCAATTATCAAAG+GGG - chr2.1:17488407-17488426 None:intergenic 35.0%
ATTATGAAGAGGTAGTAGTG+TGG + chr2.1:17488729-17488748 MS.gene35285:intron 35.0%
ATTCCAAATCAGCAGATCAT+TGG + chr2.1:17489296-17489315 MS.gene35285:CDS 35.0%
CAAAGTCCTACGTCAAATTT+AGG - chr2.1:17487267-17487286 None:intergenic 35.0%
CATATTCTTTATCTGGGCTT+GGG + chr2.1:17488212-17488231 MS.gene35285:intron 35.0%
CTAAATTTGACGTAGGACTT+TGG + chr2.1:17487265-17487284 MS.gene35285:intron 35.0%
GAACGAATTTCGAAATCCTA+GGG - chr2.1:17486098-17486117 None:intergenic 35.0%
GAATGCTTATACCTAGTAAC+TGG + chr2.1:17489032-17489051 MS.gene35285:intron 35.0%
GTTGTTAAAGGGAATACATG+GGG - chr2.1:17488571-17488590 None:intergenic 35.0%
TAGCTTTCTTGAGTTCAGTT+GGG - chr2.1:17489082-17489101 None:intergenic 35.0%
TCACCATAGAAGTTACCTAT+AGG + chr2.1:17487938-17487957 MS.gene35285:intron 35.0%
TCATATTCTTTATCTGGGCT+TGG + chr2.1:17488211-17488230 MS.gene35285:intron 35.0%
TCCCTGTGTATATATCTTGT+TGG + chr2.1:17487690-17487709 MS.gene35285:intron 35.0%
TCTAGTCAGGATAGAGAAAA+TGG - chr2.1:17488957-17488976 None:intergenic 35.0%
TGTATGTATTCCTAATGTCC+TGG + chr2.1:17487663-17487682 MS.gene35285:intron 35.0%
TTCTAAATATCGCTAACCCT+AGG + chr2.1:17486079-17486098 MS.gene35285:intron 35.0%
TTGAATGTAACCACATGTGT+TGG + chr2.1:17486822-17486841 MS.gene35285:intron 35.0%
! AAGCACTAGTCTGACTATTA+AGG - chr2.1:17486729-17486748 None:intergenic 35.0%
! AATTTAGGGCATGTTTGGAT+TGG - chr2.1:17487252-17487271 None:intergenic 35.0%
! ACAACATTTTGCAACTCTTG+GGG + chr2.1:17487619-17487638 MS.gene35285:intron 35.0%
! AGGTTAGTGCTGTTAAATAG+CGG + chr2.1:17487736-17487755 MS.gene35285:intron 35.0%
! CTACTATTTTCTGCAATCCT+TGG + chr2.1:17487899-17487918 MS.gene35285:intron 35.0%
! CTCTGAATCAGTGTTGTTAA+AGG - chr2.1:17488583-17488602 None:intergenic 35.0%
! GTGTTGTTAAAGGGAATACA+TGG - chr2.1:17488573-17488592 None:intergenic 35.0%
! TCTGCTGTTATACCAGTTTT+AGG + chr2.1:17488924-17488943 MS.gene35285:intron 35.0%
! TTACTAGCTTGGACTTAATG+TGG + chr2.1:17488064-17488083 MS.gene35285:intron 35.0%
! TTGAAATTTTTGACAGCCTC+CGG - chr2.1:17488540-17488559 None:intergenic 35.0%
!! AATGATCTGCTGATTTGGAA+TGG - chr2.1:17489297-17489316 None:intergenic 35.0%
!! CATTCAAGTTGAAAGAGGTA+TGG - chr2.1:17489017-17489036 None:intergenic 35.0%
!! GTTGTTTTCGTAAGCAATCT+TGG + chr2.1:17487114-17487133 MS.gene35285:intron 35.0%
!!! AACTTACTGTTGTCGTTTTG+TGG + chr2.1:17487796-17487815 MS.gene35285:intron 35.0%
!!! AGGTGCTAACTTTTGGAATT+TGG + chr2.1:17487970-17487989 MS.gene35285:intron 35.0%
!!! GCTGTTTTTGTAAGCTATCT+TGG + chr2.1:17486979-17486998 MS.gene35285:intron 35.0%
AAAGGGAATACATGGGGAAA+TGG - chr2.1:17488565-17488584 None:intergenic 40.0%
ACATATGTGGTGAGTGAAGT+CGG - chr2.1:17489164-17489183 None:intergenic 40.0%
ACATGCCCTAAATTTGACGT+AGG + chr2.1:17487258-17487277 MS.gene35285:intron 40.0%
AGAAAGCTATAATGCAAGCC+AGG + chr2.1:17489093-17489112 MS.gene35285:CDS 40.0%
AGAAGCTCAACGTTTCTTCA+TGG - chr2.1:17486027-17486046 None:intergenic 40.0%
ATGCCTTCGATCATAAGTGT+TGG + chr2.1:17486880-17486899 MS.gene35285:intron 40.0%
ATTTAACAGCACTAACCTCC+AGG - chr2.1:17487734-17487753 None:intergenic 40.0%
GACGTAGGACTTTGGATTAT+TGG + chr2.1:17487273-17487292 MS.gene35285:intron 40.0%
GCACCTAGTAATACACCTAT+AGG - chr2.1:17487956-17487975 None:intergenic 40.0%
GCACTTCATTGAACACTAAG+AGG + chr2.1:17487515-17487534 MS.gene35285:CDS 40.0%
GCCATAGCACTATAGTATAG+CGG + chr2.1:17487768-17487787 MS.gene35285:intron 40.0%
GGAACGAATTTCGAAATCCT+AGG - chr2.1:17486099-17486118 None:intergenic 40.0%
GTCTAGATTAAGAGCAGTGT+TGG + chr2.1:17488429-17488448 MS.gene35285:intron 40.0%
GTGTCAGTGCTAGAAAATGT+CGG + chr2.1:17486848-17486867 MS.gene35285:intron 40.0%
TATATACACAGGGAAACACC+AGG - chr2.1:17487684-17487703 None:intergenic 40.0%
TCCGCTATACTATAGTGCTA+TGG - chr2.1:17487772-17487791 None:intergenic 40.0%
TGTCCACCAATTATCAAAGG+GGG - chr2.1:17488406-17488425 None:intergenic 40.0%
! AACATTTTGCAACTCTTGGG+GGG + chr2.1:17487621-17487640 MS.gene35285:intron 40.0%
! AGTTTAGGGCATGATTGGAT+TGG - chr2.1:17486777-17486796 None:intergenic 40.0%
! ATACCCCCTTTGATAATTGG+TGG + chr2.1:17488400-17488419 MS.gene35285:intron 40.0%
! CAACATTTTGCAACTCTTGG+GGG + chr2.1:17487620-17487639 MS.gene35285:intron 40.0%
! CCAATACCCCCTTTGATAAT+TGG + chr2.1:17488397-17488416 MS.gene35285:intron 40.0%
! CCAATTATCAAAGGGGGTAT+TGG - chr2.1:17488400-17488419 None:intergenic 40.0%
! CGTCAAATTTAGGGCATGTT+TGG - chr2.1:17487257-17487276 None:intergenic 40.0%
! CTATTTTCTGCAATCCTTGG+TGG + chr2.1:17487902-17487921 MS.gene35285:intron 40.0%
! TTATGATCGAAGGCATGTCT+GGG - chr2.1:17486876-17486895 None:intergenic 40.0%
! TTGCCAATGATCTGCTGATT+TGG - chr2.1:17489302-17489321 None:intergenic 40.0%
!! ATTAAGAGCAGTGTTGGCAA+TGG + chr2.1:17488435-17488454 MS.gene35285:intron 40.0%
!!! TGTTGTCGTTTTGTGGTACA+TGG + chr2.1:17487803-17487822 MS.gene35285:intron 40.0%
ACTTGTTTGCGCAGATGATC+GGG + chr2.1:17489055-17489074 MS.gene35285:intron 45.0%
AGCTATAATGCAAGCCAGGA+TGG + chr2.1:17489097-17489116 MS.gene35285:CDS 45.0%
AGGACCCGTTTCACAAGATT+GGG + chr2.1:17485905-17485924 MS.gene35285:CDS 45.0%
AGTAAGCTTCTTGTCCATCC+TGG - chr2.1:17489114-17489133 None:intergenic 45.0%
ATTCCTTCTCAAATTGCCGG+AGG + chr2.1:17488521-17488540 MS.gene35285:intron 45.0%
CGCAAACAAGTCCAGTTACT+AGG - chr2.1:17489046-17489065 None:intergenic 45.0%
GAACACTAAGAGGCTTGATG+AGG + chr2.1:17487525-17487544 MS.gene35285:CDS 45.0%
GATGGACAAGAAGCTTACTC+AGG + chr2.1:17489115-17489134 MS.gene35285:CDS 45.0%
GCACCAACACTTATGATCGA+AGG - chr2.1:17486886-17486905 None:intergenic 45.0%
GCATTTGCAGGCAACAAGAT+AGG - chr2.1:17488696-17488715 None:intergenic 45.0%
GGCATTCCTTCTCAAATTGC+CGG + chr2.1:17488518-17488537 MS.gene35285:intron 45.0%
GGGTTCCCAATCTTGTGAAA+CGG - chr2.1:17485913-17485932 None:intergenic 45.0%
GGTTCCCAATCTTGTGAAAC+GGG - chr2.1:17485912-17485931 None:intergenic 45.0%
TCAGCACTCTGACCAGAAAA+TGG - chr2.1:17487434-17487453 None:intergenic 45.0%
TCAGCAGATCATTGGCAAGT+TGG + chr2.1:17489304-17489323 MS.gene35285:CDS 45.0%
TCATTTCTTCCCACGCAGTT+TGG - chr2.1:17489345-17489364 None:intergenic 45.0%
TCCAATCATGCCCTAAACTC+TGG + chr2.1:17486778-17486797 MS.gene35285:intron 45.0%
TGTGTGTAAGGCACGGTATA+TGG + chr2.1:17486354-17486373 MS.gene35285:intron 45.0%
! ATGCCTTCGATCAGAAGTGT+TGG + chr2.1:17487019-17487038 MS.gene35285:intron 45.0%
! CTTATGATCGAAGGCATGTC+TGG - chr2.1:17486877-17486896 None:intergenic 45.0%
! GAATTTAGCTCGTGAGTCTC+CGG + chr2.1:17487555-17487574 MS.gene35285:intron 45.0%
! GACTTGTTTGCGCAGATGAT+CGG + chr2.1:17489054-17489073 MS.gene35285:intron 45.0%
! TATGATCGAAGGCATGTCTG+GGG - chr2.1:17486875-17486894 None:intergenic 45.0%
! TCCAGAGTTTAGGGCATGAT+TGG - chr2.1:17486782-17486801 None:intergenic 45.0%
!! GTCAGAGTGCTGATTGTGTT+GGG + chr2.1:17487441-17487460 MS.gene35285:intron 45.0%
!! GTGTTCATATGGCGGAGTTT+TGG - chr2.1:17488468-17488487 None:intergenic 45.0%
!!! CACTGATTCGCTCCATTTTC+TGG + chr2.1:17487419-17487438 MS.gene35285:intron 45.0%
ATCTGGGCTTGGGATTACTG+TGG + chr2.1:17488222-17488241 MS.gene35285:intron 50.0%
CAGGACCCGTTTCACAAGAT+TGG + chr2.1:17485904-17485923 MS.gene35285:CDS 50.0%
CAGGGAAACACCAGGACATT+AGG - chr2.1:17487676-17487695 None:intergenic 50.0%
CATGGTTTCGATATCGGCAC+CGG - chr2.1:17486009-17486028 None:intergenic 50.0%
CTGACACACACCAACACATG+TGG - chr2.1:17486835-17486854 None:intergenic 50.0%
GCTCCAACACTTCTGATCGA+AGG - chr2.1:17487025-17487044 None:intergenic 50.0%
TCGTACTCCTGGATCACTTG+AGG - chr2.1:17489263-17489282 None:intergenic 50.0%
TGAGAAGCCTCAAGTGATCC+AGG + chr2.1:17489253-17489272 MS.gene35285:CDS 50.0%
TTGAGAAGGAATGCCCACCA+TGG - chr2.1:17488513-17488532 None:intergenic 50.0%
TTTGTCATCCTTCCTGGCAG+CGG - chr2.1:17485964-17485983 None:intergenic 50.0%
! ACGCCTTCGATCAGAAGTGT+CGG + chr2.1:17486549-17486568 MS.gene35285:intron 50.0%
! GACAGCTTTGTCATCCTTCC+TGG - chr2.1:17485970-17485989 None:intergenic 50.0%
! TGATCCAGGAGTACGAGTCA+GGG + chr2.1:17489267-17489286 MS.gene35285:CDS 50.0%
! TTGAAGCATGCGAGTTGACC+TGG + chr2.1:17487713-17487732 MS.gene35285:intron 50.0%
!! AAGAGCAGTGTTGGCAATGG+TGG + chr2.1:17488438-17488457 MS.gene35285:intron 50.0%
!! GGTCAGAGTGCTGATTGTGT+TGG + chr2.1:17487440-17487459 MS.gene35285:intron 50.0%
AGCTTTCTTGCGGAGGACGA+CGG - chr2.1:17485934-17485953 None:intergenic 55.0%
CAGCCTCCGGCAATTTGAGA+AGG - chr2.1:17488527-17488546 None:intergenic 55.0%
GCCATATGAACACGCCATGG+CGG + chr2.1:17488475-17488494 MS.gene35285:intron 55.0%
GCCATGGCGTGTTCATATGG+CGG - chr2.1:17488476-17488495 None:intergenic 55.0%
TCCGCCATATGAACACGCCA+TGG + chr2.1:17488472-17488491 MS.gene35285:intron 55.0%
! AAGCATGCGAGTTGACCTGG+AGG + chr2.1:17487716-17487735 MS.gene35285:intron 55.0%
! CTTCTGATCGAAGGCGTGTC+TGG - chr2.1:17486546-17486565 None:intergenic 55.0%
! CTTTCCCTGACTCGTACTCC+TGG - chr2.1:17489274-17489293 None:intergenic 55.0%
! GCACCGACACTTCTGATCGA+AGG - chr2.1:17486555-17486574 None:intergenic 55.0%
! GGCAAGTTGGAGAGAGCTCT+TGG + chr2.1:17489317-17489336 MS.gene35285:CDS 55.0%
! GTGATCCAGGAGTACGAGTC+AGG + chr2.1:17489266-17489285 MS.gene35285:CDS 55.0%
!! CTCTTGGTGCCAAACTGCGT+GGG + chr2.1:17489333-17489352 MS.gene35285:CDS 55.0%
!! GCAGTGTTGGCAATGGTGGA+AGG + chr2.1:17488442-17488461 MS.gene35285:intron 55.0%
GCCGCCATGGCGTGTTCATA+TGG - chr2.1:17488479-17488498 None:intergenic 60.0%
GCTTTCTTGCGGAGGACGAC+GGG - chr2.1:17485933-17485952 None:intergenic 60.0%
!! GCCTATGGTGCTGCCATGGT+GGG + chr2.1:17488497-17488516 MS.gene35285:intron 60.0%
!! GCTCTTGGTGCCAAACTGCG+TGG + chr2.1:17489332-17489351 MS.gene35285:CDS 60.0%
CGGCGGTGGGAGCTTTCTTG+CGG - chr2.1:17485944-17485963 None:intergenic 65.0%
CGGTGGGAGCTTTCTTGCGG+AGG - chr2.1:17485941-17485960 None:intergenic 65.0%
GAACACGCCATGGCGGCCTA+TGG + chr2.1:17488482-17488501 MS.gene35285:intron 65.0%
GCCCACCATGGCAGCACCAT+AGG - chr2.1:17488501-17488520 None:intergenic 65.0%
GTCATCCTTCCTGGCAGCGG+CGG - chr2.1:17485961-17485980 None:intergenic 65.0%
!! GGCCTATGGTGCTGCCATGG+TGG + chr2.1:17488496-17488515 MS.gene35285:intron 65.0%
ATCCTTCCTGGCAGCGGCGG+TGG - chr2.1:17485958-17485977 None:intergenic 70.0%
GGCAGCACCATAGGCCGCCA+TGG - chr2.1:17488492-17488511 None:intergenic 70.0%
GTCAACGCCGCTCGTCGTGC+CGG + chr2.1:17485987-17486006 MS.gene35285:CDS 70.0%
TCCTTCCTGGCAGCGGCGGT+GGG - chr2.1:17485957-17485976 None:intergenic 70.0%
!! ATCGGCACCGGCACGACGAG+CGG - chr2.1:17485997-17486016 None:intergenic 70.0%
!! GGCGGCCTATGGTGCTGCCA+TGG + chr2.1:17488493-17488512 MS.gene35285:intron 70.0%
AAGCTCCCACCGCCGCTGCC+AGG + chr2.1:17485949-17485968 MS.gene35285:CDS 75.0%
TCCCACCGCCGCTGCCAGGA+AGG + chr2.1:17485953-17485972 MS.gene35285:CDS 75.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 17485900 17489364 17485900 ID=MS.gene35285
chr2.1 mRNA 17485900 17489364 17485900 ID=MS.gene35285.t1;Parent=MS.gene35285
chr2.1 exon 17485900 17486035 17485900 ID=MS.gene35285.t1.exon1;Parent=MS.gene35285.t1
chr2.1 CDS 17485900 17486035 17485900 ID=cds.MS.gene35285.t1;Parent=MS.gene35285.t1
chr2.1 exon 17487482 17487565 17487482 ID=MS.gene35285.t1.exon2;Parent=MS.gene35285.t1
chr2.1 CDS 17487482 17487565 17487482 ID=cds.MS.gene35285.t1;Parent=MS.gene35285.t1
chr2.1 exon 17489069 17489151 17489069 ID=MS.gene35285.t1.exon3;Parent=MS.gene35285.t1
chr2.1 CDS 17489069 17489151 17489069 ID=cds.MS.gene35285.t1;Parent=MS.gene35285.t1
chr2.1 exon 17489245 17489364 17489245 ID=MS.gene35285.t1.exon4;Parent=MS.gene35285.t1
chr2.1 CDS 17489245 17489364 17489245 ID=cds.MS.gene35285.t1;Parent=MS.gene35285.t1
Gene Sequence

>MS.gene35285

ATGGCAGGACCCGTTTCACAAGATTGGGAACCCGTCGTCCTCCGCAAGAAAGCTCCCACCGCCGCTGCCAGGAAGGATGACAAAGCTGTCAACGCCGCTCGTCGTGCCGGTGCCGATATCGAAACCATGAAGAAACGTTGAGCTTCTTTCCTTTTCTTTCATTTCTTTAATCATTCGTTTTCTAAATATCGCTAACCCTAGGATTTCGAAATTCGTTCCTTCGTTTATTAAAATAAATTTCATGTTATCTTTTTGCTTTCGATCAAAGCGTTTAATTTGTTAAATCACTGTTTGGATTGTTGATATTTCTTATTAGGGTTTGTGTTGTTAATTTTTTTTATATTGCATTTTTTTATTTGTTATATCTATGTTAAATTTGAAATAATAAATGTTTTTTCTTGGTGATAATCTTTCATGGTTTTTCATTTCTGTTGTTGATTTTCTATTAATATGATGTGTGTAAGGCACGGTATATGGATAAACAGCTTAATTTACAGCTTATAGCACAAACACGTCTCATGATAAGCGCTTATGTTTAAACAACGACAAGTGACACCGACAATAATTTGAAAAAAAAAAAAAAAAAGAAGTGATAATTGAATGTAATCACATACGACGGCGTGTGATGCTGCAAAAACTGACACCAGACACGCCTTCGATCAGAAGTGTCGGTGCTATGTAGTGTGTAAGCTATTTCTATAACACGAGATAAAATAAAGTCACATTGTTTTCGTATAAGCTATGTTTTCATATGTTATCTTGGAGAGCTTATGAAAATAAGCTGAAACAACTTATGAACAAGTCATCAGCTGTTTTTATAAGCTCTCCTTAATAGTCAGACTAGTGCTTATGCTATTATATAAGCTCAAATAAGCCAATCCAATCATGCCCTAAACTCTGGATTATTGAAAAAAAATGAGAATTGAATGTAACCACATGTGTTGGTGTGTGTCAGTGCTAGAAAATGTCGGACCCCAGACATGCCTTCGATCATAAGTGTTGGTGCTACATATTGTATAAGCTAGTTCTATAACACGAGATAAAAGAAAGGCACATTTTTTTTTCCGTATAAGCTATTAGCTGTTTTTGTAAGCTATCTTGGAGAGTTTATGAAAATAAATGCCTTCGATCAGAAGTGTTGGAGCTACATAGTGTATAAGCTATTTCTATAACACGAGATAAATTAAATTCACATTGATTTCGTATAATCTATGGTTGTTTTCGTAAGCAATCTTGGATAGCTTATGAAAATAAGCTGAAAACAACTCATGAACAAGTCATCAGCTGTTTTCATAAGTTCTCCTTCATAGTCATACAAGTGCTTATGCTATTATATAAGCTCAAATAAGCCAATCCAAACATGCCCTAAATTTGACGTAGGACTTTGGATTATTGGATTTTCAAGACTCTAACACAAATAGTTTATGAGCAGTTTTCTGCAAGTTGTTTTTGTAATTATGAGCAGTTTTTTTCGTTGATTGATCTTTTACTTGTATAATTGAATGTTTCTCATTTTTATCACTGATTCGCTCCATTTTCTGGTCAGAGTGCTGATTGTGTTGGGTTGTTCTATACATTACAGATAATGCTGCGACAAACAAAGCTGCTTCTAGCAGCACTTCATTGAACACTAAGAGGCTTGATGAGGATACAGAGAATTTAGCTCGTGAGTCTCCGGTTACTCTATTATTTTTTTTCTTCCCATTTTATAATATTGAAACAACATTTTGCAACTCTTGGGGGGTTTAACTTTTACAATGTATTGTATGTATTCCTAATGTCCTGGTGTTTCCCTGTGTATATATCTTGTTGGTTGAAGCATGCGAGTTGACCTGGAGGTTAGTGCTGTTAAATAGCGGTATAGTAGTGCCATAGCACTATAGTATAGCGGAATTTAACTTACTGTTGTCGTTTTGTGGTACATGGTTTAGTTCTAAGTGTTGTCAAATAGTGGCTATAGCTGTGACATAACATTGAAGCATAACATGATTTGAACAAACTACTATTTTCTGCAATCCTTGGTGGAAGTAAAACTTTTTCACCATAGAAGTTACCTATAGGTGTATTACTAGGTGCTAACTTTTGGAATTTGGAATATCATAAGACTGTTTGGAAGTGCTATATGTATATATCTTCCATAATTAATTGTGGAATTCATATTCTTTTACTAGCTTGGACTTAATGTGGATATAACATGTGGTTTTTTACAATATTCTTTTATTAGCCTGGATTTACTTTGCTCAATTTTGGAATATCGTATGAAAGTTCATGGTATTGTTATCTGCAATTATTACATAATTACTGAGAAAATTCATATTCTTTATCTGGGCTTGGGATTACTGTGGACATGTGTACTCTTTGTTGTTTGATTTCATATCTCAGATTTTTGTTATATTAATATTATATATGTTTACAATAATTCTTTTTATCACAATGCCAAAAATGTCTTCATAGTTTGGACATGAGAATGTGTGACTGTACTGTGTTCTAGTGTAGTCCAATACCCCCTTTGATAATTGGTGGACATTAGTCTAGATTAAGAGCAGTGTTGGCAATGGTGGAAGGCCAAAACTCCGCCATATGAACACGCCATGGCGGCCTATGGTGCTGCCATGGTGGGCATTCCTTCTCAAATTGCCGGAGGCTGTCAAAAATTTCAATGCCATTTCCCCATGTATTCCCTTTAACAACACTGATTCAGAGATCAGAGCTCTCGAGACTGATTCAGATCTGATTTAGAGCTTTATAATAGTAATCCCCATCCTAATCAAATATTTTTCTACAAAATGATATCCTATCTTGTTGCCTGCAAATGCAATTCTATTATAAATTATGAAGAGGTAGTAGTGTGGTCTACCACAGCAATTATAAAAATGCATCTCAGTTTACAATGTAGAATCTGTTGCTTGTGCTTTATTTCTGTTTGACGAATGACAAAATAGAAATTTGTAGTGTTCAGCAATTCAAACTTATGTATTTACAAAGAGAGGGGCTTTGTTTAACAGAGCAGTGTAGCATGCTAGCTCTGCTGTTATACCAGTTTTAGGTTTTGATCCATTTTCTCTATCCTGACTAGAATTTTTAGTGGAACATCAACTCCCTTTTATATAAGAGCCATACCTCTTTCAACTTGAATGCTTATACCTAGTAACTGGACTTGTTTGCGCAGATGATCGGGTCCCAACTGAACTCAAGAAAGCTATAATGCAAGCCAGGATGGACAAGAAGCTTACTCAGGCACAACTTGCTCAAGTATTGCTGCCGACTTCACTCACCACATATGTTTTTTTGTCTTGATTTCATTATTTGAAGTTTGATATTAATAACGAGCATGTTTGATTGCAGATTATCAATGAGAAGCCTCAAGTGATCCAGGAGTACGAGTCAGGGAAAGCCATTCCAAATCAGCAGATCATTGGCAAGTTGGAGAGAGCTCTTGGTGCCAAACTGCGTGGGAAGAAATGA
Protein sequence

>MS.gene35285.t1

MAGPVSQDWEPVVLRKKAPTAAARKDDKAVNAARRAGADIETMKKHNAATNKAASSSTSLNTKRLDEDTENLAHDRVPTELKKAIMQARMDKKLTQAQLAQIINEKPQVIQEYESGKAIPNQQIIGKLERALGAKLRGKK