AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene043260


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043260.t1 MTR_3g056705 92.347 196 15 0 6 201 872 1067 2.90e-120 373
MS.gene043260.t1 MTR_5g094830 68.367 196 51 2 6 201 825 1009 2.23e-82 267
MS.gene043260.t1 MTR_5g033030 66.837 196 60 3 6 201 814 1004 3.99e-80 260
MS.gene043260.t1 MTR_5g094810 67.005 197 52 3 6 201 810 994 9.41e-80 259
MS.gene043260.t1 MTR_5g033320 64.286 196 64 4 6 201 805 994 8.37e-75 245
MS.gene043260.t1 MTR_3g056700 50.242 207 101 2 6 212 625 829 2.25e-62 209
MS.gene043260.t1 MTR_3g056675 49.275 207 103 2 6 212 572 776 6.44e-58 197
MS.gene043260.t1 MTR_3g056645 49.515 206 102 2 7 212 1 204 6.50e-58 194
MS.gene043260.t1 MTR_7g098690 39.462 223 117 3 6 212 818 1038 1.03e-42 153
MS.gene043260.t1 MTR_1g069450 42.105 209 103 3 6 200 838 1042 3.10e-42 152
MS.gene043260.t1 MTR_1g088680 31.156 199 121 4 6 200 845 1031 9.04e-26 104
MS.gene043260.t1 MTR_8g080050 33.673 196 107 6 6 200 783 956 2.85e-25 103
MS.gene043260.t1 MTR_8g015970 31.731 208 129 4 6 212 615 810 1.58e-23 98.2
MS.gene043260.t1 MTR_8g015970 31.731 208 129 4 6 212 615 810 1.70e-23 98.2
MS.gene043260.t1 MTR_8g015970 31.731 208 129 4 6 212 619 814 1.82e-23 98.2
MS.gene043260.t1 MTR_8g015970 31.731 208 129 4 6 212 619 814 1.86e-23 98.2
MS.gene043260.t1 MTR_6g084320 28.986 207 123 4 6 212 782 964 3.13e-22 94.7
MS.gene043260.t1 MTR_6g084320 28.986 207 123 4 6 212 782 964 4.44e-22 94.0
MS.gene043260.t1 MTR_2g436710 30.102 196 124 4 6 200 836 1019 2.46e-21 92.0
MS.gene043260.t1 MTR_8g015980 27.830 212 121 4 6 212 791 975 3.12e-20 89.0
MS.gene043260.t1 MTR_8g016020 28.986 207 126 5 6 212 573 758 3.81e-20 88.6
MS.gene043260.t1 MTR_8g040170 28.571 210 133 4 6 212 444 639 4.47e-20 88.2
MS.gene043260.t1 MTR_8g040170 28.571 210 133 4 6 212 604 799 4.60e-20 88.2
MS.gene043260.t1 MTR_8g040620 29.469 207 127 4 6 212 794 981 1.03e-19 87.4
MS.gene043260.t1 MTR_6g034270 27.979 193 116 4 6 198 633 802 8.72e-19 84.7
MS.gene043260.t1 MTR_8g016010 27.536 207 139 2 6 212 146 341 1.26e-18 84.0
MS.gene043260.t1 MTR_6g034335 26.667 195 121 4 6 200 820 992 5.75e-18 82.4
MS.gene043260.t1 MTR_6g034230 27.692 195 118 4 6 200 816 987 1.08e-17 81.3
MS.gene043260.t1 MTR_8g016070 31.472 197 110 5 6 198 801 976 1.28e-17 81.3
MS.gene043260.t1 MTR_2g436730 27.778 198 121 4 6 197 834 1015 3.27e-17 80.1
MS.gene043260.t1 MTR_1g099280 29.231 195 118 4 6 200 814 988 4.05e-17 79.7
MS.gene043260.t1 MTR_8g061970 27.094 203 123 4 6 200 831 1016 4.07e-17 79.7
MS.gene043260.t1 MTR_1g099280 29.231 195 118 4 6 200 839 1013 4.07e-17 79.7
MS.gene043260.t1 MTR_1g099280 29.231 195 118 4 6 200 814 988 4.64e-17 79.7
MS.gene043260.t1 MTR_0196s0020 26.396 197 118 5 6 200 816 987 1.99e-16 77.8
MS.gene043260.t1 MTR_2g436680 27.692 195 127 3 6 200 836 1016 8.80e-16 75.9
MS.gene043260.t1 MTR_6g034265 26.667 195 120 4 6 200 814 985 1.19e-15 75.5
MS.gene043260.t1 MTR_6g034755 25.128 195 124 3 6 200 823 995 2.23e-15 74.7
MS.gene043260.t1 MTR_6g034310 26.667 195 117 4 6 200 802 970 9.72e-15 72.8
MS.gene043260.t1 MTR_0019s0020 27.179 195 119 4 6 200 766 937 9.96e-15 72.8
MS.gene043260.t1 MTR_0019s0020 27.179 195 119 4 6 200 766 937 9.97e-15 72.8
MS.gene043260.t1 MTR_0019s0020 27.179 195 119 4 6 200 766 937 1.01e-14 72.8
MS.gene043260.t1 MTR_0019s0020 27.179 195 119 4 6 200 766 937 1.01e-14 72.8
MS.gene043260.t1 MTR_6g034310 27.083 192 114 4 6 197 444 609 1.02e-14 72.8
MS.gene043260.t1 MTR_0019s0020 27.179 195 119 4 6 200 766 937 1.16e-14 72.4
MS.gene043260.t1 MTR_6g034310 26.667 195 117 4 6 200 802 970 1.22e-14 72.4
MS.gene043260.t1 MTR_8g042520 26.562 192 115 5 6 197 13 178 4.04e-14 70.9
MS.gene043260.t1 MTR_6g034220 27.692 195 118 4 6 200 815 986 7.34e-14 70.1
MS.gene043260.t1 MTR_6g034350 23.077 195 128 3 6 200 823 995 5.84e-13 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043260.t1 AT1G71330 57.005 207 86 3 6 212 107 310 2.10e-77 235
MS.gene043260.t1 AT3G13080 57.488 207 85 3 6 212 835 1038 1.63e-75 246
MS.gene043260.t1 AT3G13080 57.488 207 85 3 6 212 835 1038 1.63e-75 246
MS.gene043260.t1 AT3G13080 57.488 207 85 3 6 212 835 1038 4.42e-75 246
MS.gene043260.t1 AT3G13080 57.488 207 85 3 6 212 835 1038 4.63e-75 246
MS.gene043260.t1 AT3G13080 57.488 207 85 3 6 212 835 1038 4.85e-75 246
MS.gene043260.t1 AT3G13100 56.039 207 77 3 6 212 819 1011 8.39e-66 219
MS.gene043260.t1 AT3G13100 56.039 207 77 3 6 212 574 766 8.86e-66 219
MS.gene043260.t1 AT3G13090 52.174 207 86 3 6 212 796 989 9.37e-64 214
MS.gene043260.t1 AT1G04120 41.038 212 106 4 6 200 782 991 1.07e-39 144
MS.gene043260.t1 AT1G04120 41.038 212 106 4 6 200 817 1026 1.21e-39 144
MS.gene043260.t1 AT1G04120 41.038 212 106 4 6 200 817 1026 1.23e-39 144
MS.gene043260.t1 AT3G60160 36.410 195 118 2 6 200 825 1013 3.58e-34 129
MS.gene043260.t1 AT3G60160 36.410 195 118 2 6 200 825 1013 4.26e-34 129
MS.gene043260.t1 AT3G60160 36.410 195 118 2 6 200 789 977 4.29e-34 129
MS.gene043260.t1 AT3G60970 34.872 195 84 3 6 200 409 560 5.46e-27 108
MS.gene043260.t1 AT2G47800 29.808 208 128 4 3 200 833 1032 8.11e-23 96.3
MS.gene043260.t1 AT3G62700 29.018 224 128 4 6 200 834 1055 1.34e-20 90.1
MS.gene043260.t1 AT3G21250 30.542 203 109 4 5 198 787 966 3.48e-17 80.1
MS.gene043260.t1 AT3G21250 30.542 203 109 4 5 198 781 960 3.86e-17 79.7
MS.gene043260.t1 AT3G21250 29.630 216 120 4 6 212 793 985 4.85e-17 79.7
MS.gene043260.t1 AT3G21250 29.630 216 120 4 6 212 799 991 5.00e-17 79.7
MS.gene043260.t1 AT3G21250 29.630 216 120 4 6 212 377 569 5.57e-17 79.3
MS.gene043260.t1 AT3G21250 29.630 216 120 4 6 212 377 569 5.57e-17 79.3
MS.gene043260.t1 AT3G59140 26.667 195 117 4 6 200 797 965 1.81e-16 77.8

Find 32 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAAATTGGAAGCAACTAT+TGG 0.238628 7.2:+87810777 MS.gene043260:CDS
ATGTTTCAACTTCTTCAAAT+TGG 0.277828 7.2:+87810764 MS.gene043260:CDS
GAAAGAGAGCAAGGTAAAGT+TGG 0.353443 7.2:+87810665 MS.gene043260:CDS
TCTTGAACAAGTTGTCGTTT+TGG 0.370037 7.2:-87810638 None:intergenic
AAAGAGAGCAAGGTAAAGTT+GGG 0.433227 7.2:+87810666 MS.gene043260:CDS
CCTGTTGTGATGTACTTATC+TGG 0.444623 7.2:-87810529 None:intergenic
AAATATATCACGACAGCATA+TGG 0.450488 7.2:+87810704 MS.gene043260:CDS
CCAGATAAGTACATCACAAC+AGG 0.482922 7.2:+87810529 MS.gene043260:CDS
AGGAGTGTAGTTCCTCGAAC+AGG 0.499646 7.2:-87810839 None:intergenic
TCTGCACTTGATTCATCAGA+TGG 0.504417 7.2:+87810488 MS.gene043260:CDS
AATTGGAAGCAACTATTGGA+TGG 0.509516 7.2:+87810781 MS.gene043260:CDS
CAACAAGAGTTGCTCTAGAT+AGG 0.511129 7.2:-87810904 None:intergenic
CCATGTGTCTCTCTTCCTCT+CGG 0.512974 7.2:+87810554 MS.gene043260:CDS
GCAACTCTTGTTGTCACCGT+TGG 0.514424 7.2:+87810914 MS.gene043260:CDS
GACACATAAAGAACTTCCTA+TGG 0.530280 7.2:-87810882 None:intergenic
GAGAAGAAAGAAGTACAGAA+TGG 0.536785 7.2:+87810593 MS.gene043260:CDS
GATGTGGAGCCACCTGTTCG+AGG 0.554467 7.2:+87810827 MS.gene043260:CDS
CCACACCTACATAGACAACT+AGG 0.557019 7.2:-87810859 None:intergenic
AACTCCCATCTCAAAAGATG+TGG 0.570549 7.2:+87810811 MS.gene043260:CDS
CAAGAAGAAGAAAGAGAGCA+AGG 0.570892 7.2:+87810656 MS.gene043260:CDS
CCTAGTTGTCTATGTAGGTG+TGG 0.574400 7.2:+87810859 MS.gene043260:CDS
ACACTCCTAGTTGTCTATGT+AGG 0.580169 7.2:+87810854 MS.gene043260:CDS
GATGGGGAAATTACTCAATG+TGG 0.583724 7.2:+87810395 MS.gene043260:CDS
AAGAAAGAAGTACAGAATGG+TGG 0.595879 7.2:+87810596 MS.gene043260:CDS
TATAATGATCTACTCAACTC+AGG 0.598290 7.2:+87810422 MS.gene043260:CDS
CTTGATTCATCAGATGGAGA+TGG 0.618017 7.2:+87810494 MS.gene043260:CDS
GTCTATGTAGGTGTGGCCAT+AGG 0.623766 7.2:+87810866 MS.gene043260:CDS
CCGAGAGGAAGAGAGACACA+TGG 0.627029 7.2:-87810554 None:intergenic
AGTGTAGTTCCTCGAACAGG+TGG 0.627973 7.2:-87810836 None:intergenic
ATTTCAGCTATCAATATGAG+TGG 0.654447 7.2:-87810740 None:intergenic
AGTAGCTGTCTTATAACCAA+CGG 0.661479 7.2:-87810930 None:intergenic
TATATCACGACAGCATATGG+AGG 0.683585 7.2:+87810707 MS.gene043260:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACAAATTTTATGGAACTTAT+TGG + chr7.2:87810446-87810465 MS.gene043260:CDS 20.0%
! ATGTTTCAACTTCTTCAAAT+TGG + chr7.2:87810764-87810783 MS.gene043260:CDS 25.0%
!!! GTTTTAAGGTTATGAAAGAT+GGG + chr7.2:87810378-87810397 MS.gene043260:CDS 25.0%
!!! TGTTTTAAGGTTATGAAAGA+TGG + chr7.2:87810377-87810396 MS.gene043260:CDS 25.0%
!!! TTTTAAGGTTATGAAAGATG+GGG + chr7.2:87810379-87810398 MS.gene043260:CDS 25.0%
AAATATATCACGACAGCATA+TGG + chr7.2:87810704-87810723 MS.gene043260:CDS 30.0%
ATTTCAGCTATCAATATGAG+TGG - chr7.2:87810743-87810762 None:intergenic 30.0%
TATAATGATCTACTCAACTC+AGG + chr7.2:87810422-87810441 MS.gene043260:CDS 30.0%
TTCAAATTGGAAGCAACTAT+TGG + chr7.2:87810777-87810796 MS.gene043260:CDS 30.0%
AAAGAGAGCAAGGTAAAGTT+GGG + chr7.2:87810666-87810685 MS.gene043260:CDS 35.0%
AATTGGAAGCAACTATTGGA+TGG + chr7.2:87810781-87810800 MS.gene043260:CDS 35.0%
AGTAGCTGTCTTATAACCAA+CGG - chr7.2:87810933-87810952 None:intergenic 35.0%
GACACATAAAGAACTTCCTA+TGG - chr7.2:87810885-87810904 None:intergenic 35.0%
! AAGAAAGAAGTACAGAATGG+TGG + chr7.2:87810596-87810615 MS.gene043260:CDS 35.0%
! CAACTCAGGCACAAATTTTA+TGG + chr7.2:87810436-87810455 MS.gene043260:CDS 35.0%
! GAGAAGAAAGAAGTACAGAA+TGG + chr7.2:87810593-87810612 MS.gene043260:CDS 35.0%
!! TCTTGAACAAGTTGTCGTTT+TGG - chr7.2:87810641-87810660 None:intergenic 35.0%
AACTCCCATCTCAAAAGATG+TGG + chr7.2:87810811-87810830 MS.gene043260:CDS 40.0%
ACACTCCTAGTTGTCTATGT+AGG + chr7.2:87810854-87810873 MS.gene043260:CDS 40.0%
CAAGAAGAAGAAAGAGAGCA+AGG + chr7.2:87810656-87810675 MS.gene043260:CDS 40.0%
CCAGATAAGTACATCACAAC+AGG + chr7.2:87810529-87810548 MS.gene043260:CDS 40.0%
CCTGTTGTGATGTACTTATC+TGG - chr7.2:87810532-87810551 None:intergenic 40.0%
CTTGATTCATCAGATGGAGA+TGG + chr7.2:87810494-87810513 MS.gene043260:CDS 40.0%
GAAAGAGAGCAAGGTAAAGT+TGG + chr7.2:87810665-87810684 MS.gene043260:CDS 40.0%
GATGGGGAAATTACTCAATG+TGG + chr7.2:87810395-87810414 MS.gene043260:CDS 40.0%
TATATCACGACAGCATATGG+AGG + chr7.2:87810707-87810726 MS.gene043260:CDS 40.0%
TCTGCACTTGATTCATCAGA+TGG + chr7.2:87810488-87810507 MS.gene043260:CDS 40.0%
! CAACAAGAGTTGCTCTAGAT+AGG - chr7.2:87810907-87810926 None:intergenic 40.0%
! GCAAGGTAAAGTTGGGTTTT+CGG + chr7.2:87810673-87810692 MS.gene043260:CDS 40.0%
! TCTATTTTGTCAACACCGAG+AGG - chr7.2:87810572-87810591 None:intergenic 40.0%
CCACACCTACATAGACAACT+AGG - chr7.2:87810862-87810881 None:intergenic 45.0%
CCTAGTTGTCTATGTAGGTG+TGG + chr7.2:87810859-87810878 MS.gene043260:CDS 45.0%
!! AAGTTGGGTTTTCGGTGTAC+TGG + chr7.2:87810681-87810700 MS.gene043260:CDS 45.0%
!! TGGCTCCACATCTTTTGAGA+TGG - chr7.2:87810819-87810838 None:intergenic 45.0%
!!! GGCTCCACATCTTTTGAGAT+GGG - chr7.2:87810818-87810837 None:intergenic 45.0%
AGTGTAGTTCCTCGAACAGG+TGG - chr7.2:87810839-87810858 None:intergenic 50.0%
CCATGTGTCTCTCTTCCTCT+CGG + chr7.2:87810554-87810573 MS.gene043260:CDS 50.0%
GTCTATGTAGGTGTGGCCAT+AGG + chr7.2:87810866-87810885 MS.gene043260:CDS 50.0%
TTGGAGCGCGAAAAATGCAG+AGG - chr7.2:87810977-87810996 None:intergenic 50.0%
! AGGAGTGTAGTTCCTCGAAC+AGG - chr7.2:87810842-87810861 None:intergenic 50.0%
! GCAACTCTTGTTGTCACCGT+TGG + chr7.2:87810914-87810933 MS.gene043260:CDS 50.0%
CCGAGAGGAAGAGAGACACA+TGG - chr7.2:87810557-87810576 None:intergenic 55.0%
GATGTGGAGCCACCTGTTCG+AGG + chr7.2:87810827-87810846 MS.gene043260:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 87810371 87811009 87810371 ID=MS.gene043260
chr7.2 mRNA 87810371 87811009 87810371 ID=MS.gene043260.t1;Parent=MS.gene043260
chr7.2 exon 87810371 87811009 87810371 ID=MS.gene043260.t1.exon1;Parent=MS.gene043260.t1
chr7.2 CDS 87810371 87811009 87810371 ID=cds.MS.gene043260.t1;Parent=MS.gene043260.t1
Gene Sequence

>MS.gene043260

ATGCATTGTTTTAAGGTTATGAAAGATGGGGAAATTACTCAATGTGGAAAGTATAATGATCTACTCAACTCAGGCACAAATTTTATGGAACTTATTGGTGCTCATAGAGACGCATTGTCTGCACTTGATTCATCAGATGGAGATGGTACAATATCTGTCCAGATAAGTACATCACAACAGGATCCATGTGTCTCTCTTCCTCTCGGTGTTGACAAAATAGAAGAGAAGAAAGAAGTACAGAATGGTGGAACAAATGACGAGATTGAGCCAAAACGACAACTTGTTCAAGAAGAAGAAAGAGAGCAAGGTAAAGTTGGGTTTTCGGTGTACTGGAAATATATCACGACAGCATATGGAGGTGCTCTTGTGCCACTCATATTGATAGCTGAAATCATGTTTCAACTTCTTCAAATTGGAAGCAACTATTGGATGGCTTCTTCAACTCCCATCTCAAAAGATGTGGAGCCACCTGTTCGAGGAACTACACTCCTAGTTGTCTATGTAGGTGTGGCCATAGGAAGTTCTTTATGTGTCCTATCTAGAGCAACTCTTGTTGTCACCGTTGGTTATAAGACAGCTACTCTACTCTTCAACAAAATGCATCCTCTGCATTTTTCGCGCTCCAATGTCATTTTTTGA

Protein sequence

>MS.gene043260.t1

MHCFKVMKDGEITQCGKYNDLLNSGTNFMELIGAHRDALSALDSSDGDGTISVQISTSQQDPCVSLPLGVDKIEEKKEVQNGGTNDEIEPKRQLVQEEEREQGKVGFSVYWKYITTAYGGALVPLILIAEIMFQLLQIGSNYWMASSTPISKDVEPPVRGTTLLVVYVGVAIGSSLCVLSRATLVVTVGYKTATLLFNKMHPLHFSRSNVIF