AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene88462

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88462.t1 MTR_8g039590 87.755 49 4 1 324 370 26 74 1.25e-21 89.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88462.t1 AT1G72050 54.897 388 116 3 28 359 33 417 3.20e-142 410
MS.gene88462.t1 AT1G72050 54.897 388 116 3 28 359 18 402 3.37e-142 410
MS.gene88462.t1 AT1G72050 52.716 313 97 1 98 359 2 314 1.70e-108 321
MS.gene88462.t1 AT1G72050 50.000 276 87 1 98 322 2 277 5.22e-89 271

Find 79 sgRNAs with CRISPR-Local

Find 227 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCCTTCAAGTGCGGCTTTC+CGG 0.171296 8.3:+57810927 MS.gene88462:CDS
GCCTTCCCACAGCCAGTTTC+CGG 0.199994 8.3:-57810116 None:intergenic
GGTGTTTCATTGTGATTATA+AGG 0.329936 8.3:+57810499 MS.gene88462:CDS
AAGAAACTGATGAACAATTC+AGG 0.331946 8.3:+57811115 MS.gene88462:CDS
CACCTCCGTAAACATGAAAA+TGG 0.338406 8.3:+57810464 MS.gene88462:CDS
ACAATTCAGGTCAAGGAATA+GGG 0.340181 8.3:+57811128 MS.gene88462:CDS
CTTTACATGCTTACAAAGAT+TGG 0.344658 8.3:-57810884 None:intergenic
CCTTCCCACAGCCAGTTTCC+GGG 0.360391 8.3:-57810115 None:intergenic
AGGATTTAGTGTGTGCTCTA+AGG 0.367395 8.3:-57810379 None:intergenic
TAATGTTTCTTAGGTAGAAA+TGG 0.368064 8.3:+57809096 MS.gene88462:intron
CAGCACGTGTGCCCGGAAAC+TGG 0.376933 8.3:+57810104 MS.gene88462:CDS
AACAATTCAGGTCAAGGAAT+AGG 0.379074 8.3:+57811127 MS.gene88462:CDS
TTGTACTATTGATGATTGTC+AGG 0.388063 8.3:+57809902 MS.gene88462:CDS
ATTGATGTGAGAGGTAATTA+AGG 0.392920 8.3:-57807452 None:intergenic
GTCAATACTGTGGCATCTCC+AGG 0.399551 8.3:+57807419 MS.gene88462:CDS
TCATCAATAGTACAAATATA+CGG 0.420167 8.3:-57809894 None:intergenic
TAAGCAACTCTAGTTGAGTT+GGG 0.420854 8.3:-57811209 None:intergenic
TCGCGGTTGCAATTCTCAAT+TGG 0.423592 8.3:-57809993 None:intergenic
TGAAGGGTTTATCTTGCAAA+TGG 0.427534 8.3:-57810912 None:intergenic
GTACAAAATTGAATACTTGT+AGG 0.431705 8.3:+57809155 MS.gene88462:CDS
CTACAGTTGAAATGTTGGTT+AGG 0.459027 8.3:+57811172 MS.gene88462:CDS
ATCCGGAAAGCCGCACTTGA+AGG 0.468434 8.3:-57810929 None:intergenic
GACTTTGCATATGCTGCAAT+AGG 0.471700 8.3:-57809210 None:intergenic
GAATCATCCATCCCTGTGTT+CGG 0.472511 8.3:-57807373 None:intergenic
AAGTCTCACAGTTCACATAC+TGG 0.472897 8.3:-57810406 None:intergenic
ATAAGCAACTCTAGTTGAGT+TGG 0.481173 8.3:-57811210 None:intergenic
TCAGAAGCAGCACGTGTGCC+CGG 0.481855 8.3:+57810097 MS.gene88462:CDS
CGTCATCTTCTACAACATGA+TGG 0.491419 8.3:+57809954 MS.gene88462:CDS
ATGTGAACTGTGAGACTTGT+GGG 0.494220 8.3:+57810411 MS.gene88462:CDS
GCAAATCTCATGCCACAATC+CGG 0.501297 8.3:-57810946 None:intergenic
GTAAAACTCTTCATACAGCC+AGG 0.508670 8.3:-57810344 None:intergenic
TACAAAATTGAATACTTGTA+GGG 0.508830 8.3:+57809156 MS.gene88462:CDS
TGAAAGAGATCAAGAAGCAC+AGG 0.510409 8.3:+57809132 MS.gene88462:CDS
TCAACTAGAGTTGCTTATGA+AGG 0.516835 8.3:+57811215 MS.gene88462:CDS
GAGGTAGTGTGCTTATACCC+TGG 0.518727 8.3:+57810326 MS.gene88462:CDS
AGGATTTCTTACACTTGAGA+AGG 0.521556 8.3:-57810534 None:intergenic
GTCATCTTCTACAACATGAT+GGG 0.523277 8.3:+57809955 MS.gene88462:CDS
GTAGAAGATGACGATTCAAA+TGG 0.524054 8.3:-57809944 None:intergenic
CTAACCAACATTTCAACTGT+AGG 0.530712 8.3:-57811171 None:intergenic
AAGTGCGGCTTTCCGGATTG+TGG 0.534748 8.3:+57810934 MS.gene88462:CDS
CAATCTTTGTAAGCATGTAA+AGG 0.534955 8.3:+57810885 MS.gene88462:CDS
ATATGCTGCAATAGGTAGGC+AGG 0.535542 8.3:-57809202 None:intergenic
CCCGGAAACTGGCTGTGGGA+AGG 0.537986 8.3:+57810115 MS.gene88462:CDS
GCATTTCGTGATTGCTTCTG+CGG 0.544786 8.3:-57807351 None:intergenic
GAGGGTAAAGACATGCGAAC+CGG 0.547091 8.3:-57811001 None:intergenic
GTGTGCCCGGAAACTGGCTG+TGG 0.547157 8.3:+57810110 MS.gene88462:CDS
GTGTCCTACAGTTGAAATGT+TGG 0.550000 8.3:+57811167 MS.gene88462:CDS
TCCGGAAAGCCGCACTTGAA+GGG 0.553092 8.3:-57810928 None:intergenic
AGTTAAGCTGGAATCAATAG+AGG 0.553788 8.3:+57810307 MS.gene88462:intron
ATCACGAAATGCCGAACACA+GGG 0.569190 8.3:+57807362 MS.gene88462:CDS
TCAGGCAAGTTACAGAAGAA+AGG 0.570386 8.3:+57809920 MS.gene88462:CDS
AATCACGAAATGCCGAACAC+AGG 0.575106 8.3:+57807361 MS.gene88462:CDS
CTGATGAACAATTCAGGTCA+AGG 0.577968 8.3:+57811121 MS.gene88462:CDS
ACAGTTCACATACTGGTGGC+AGG 0.578620 8.3:-57810399 None:intergenic
AAGCAACTCTAGTTGAGTTG+GGG 0.587740 8.3:-57811208 None:intergenic
CAACTAGAGTTGCTTATGAA+GGG 0.588443 8.3:+57811216 MS.gene88462:CDS
CAATTCAGGTCAAGGAATAG+GGG 0.594620 8.3:+57811129 MS.gene88462:CDS
TTCAGGTCAAGGAATAGGGG+AGG 0.598888 8.3:+57811132 MS.gene88462:CDS
CAAGATAAACCCTTCAAGTG+CGG 0.599296 8.3:+57810919 MS.gene88462:CDS
CGAAATGCCGAACACAGGGA+TGG 0.600088 8.3:+57807366 MS.gene88462:CDS
TTTGCTTCGAGTTCTTCCGA+TGG 0.602164 8.3:-57807328 None:intergenic
TTGCATATGCTGCAATAGGT+AGG 0.603520 8.3:-57809206 None:intergenic
AAACATGACAAGACATGTCA+AGG 0.604864 8.3:+57810031 MS.gene88462:CDS
GAGAAGCACAAAGATTCTCA+TGG 0.608696 8.3:+57810161 MS.gene88462:CDS
TAACAAACTCAGAATGCCAT+CGG 0.620391 8.3:+57807312 None:intergenic
TGTCAAGGAGTTTCATAACA+AGG 0.621209 8.3:+57810046 MS.gene88462:CDS
TTCCCTTCAATGAAAAGTCG+CGG 0.629861 8.3:-57810010 None:intergenic
TGAAAATGGTAGTTCATCAG+AGG 0.635036 8.3:+57810478 MS.gene88462:CDS
TCATCTTCTACAACATGATG+GGG 0.636389 8.3:+57809956 MS.gene88462:CDS
AGCAACTCTAGTTGAGTTGG+GGG 0.637296 8.3:-57811207 None:intergenic
TATGCAAAGTCATTCACTTG+AGG 0.637403 8.3:+57809222 MS.gene88462:CDS
TTGAATACTTGTAGGGAATG+TGG 0.640544 8.3:+57809163 MS.gene88462:CDS
TAAAACTCTTCATACAGCCA+GGG 0.647065 8.3:-57810343 None:intergenic
TCAGGTCAAGGAATAGGGGA+GGG 0.649781 8.3:+57811133 MS.gene88462:CDS
TGTGCCCGGAAACTGGCTGT+GGG 0.676199 8.3:+57810111 MS.gene88462:CDS
TCTCACAGTTCACATACTGG+TGG 0.715768 8.3:-57810403 None:intergenic
GAAAGAGATCAAGAAGCACA+GGG 0.736934 8.3:+57809133 MS.gene88462:CDS
TATGTGAACTGTGAGACTTG+TGG 0.750818 8.3:+57810410 MS.gene88462:CDS
ATGATGAGAATTGATGTGAG+AGG 0.763772 8.3:-57807461 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATATAATGAGATAAAT+CGG - chr8.3:57809296-57809315 None:intergenic 10.0%
!! GTATAGATAAAAAAAAAAAA+GGG + chr8.3:57809397-57809416 MS.gene88462:intron 10.0%
!! TGTATAGATAAAAAAAAAAA+AGG + chr8.3:57809396-57809415 MS.gene88462:intron 10.0%
!! TATCTCATTATATATTTCTT+TGG + chr8.3:57809299-57809318 MS.gene88462:intron 15.0%
!! TATTTATACTATTATCCATT+AGG + chr8.3:57808032-57808051 MS.gene88462:intron 15.0%
!!! CTTATTAAGTTATTAGTTTA+TGG + chr8.3:57809833-57809852 MS.gene88462:intron 15.0%
!!! TTTTTTGAAATTCATTTCAA+TGG + chr8.3:57809353-57809372 MS.gene88462:intron 15.0%
!! AATGAATGAATTAGATGTAA+TGG - chr8.3:57807787-57807806 None:intergenic 20.0%
!! ATACTGAATTTACAAAATAG+AGG - chr8.3:57808863-57808882 None:intergenic 20.0%
!! TAAATATGATTTGATATCTC+CGG + chr8.3:57807696-57807715 MS.gene88462:intron 20.0%
!! TAATATAACAGATTCTTTAC+AGG - chr8.3:57811041-57811060 None:intergenic 20.0%
!! TACAAAATTGAATACTTGTA+GGG + chr8.3:57809156-57809175 MS.gene88462:CDS 20.0%
!! TACTGAATTTACAAAATAGA+GGG - chr8.3:57808862-57808881 None:intergenic 20.0%
!! TCATCAATAGTACAAATATA+CGG - chr8.3:57809897-57809916 None:intergenic 20.0%
!! TGTAATTGTATACAAATTTG+TGG + chr8.3:57808115-57808134 MS.gene88462:intron 20.0%
!! TTATAATTAGTTCATGCTTT+AGG + chr8.3:57809423-57809442 MS.gene88462:intron 20.0%
!! TTATCTATACAGAAATTTCA+AGG - chr8.3:57809387-57809406 None:intergenic 20.0%
!!! CAGCAATTTGTATTTTATTT+TGG - chr8.3:57807595-57807614 None:intergenic 20.0%
!!! CTTTAGATTTTGTTAAAATC+TGG + chr8.3:57809449-57809468 MS.gene88462:intron 20.0%
!!! TAACAACATTGTTTTAACTA+CGG + chr8.3:57810785-57810804 MS.gene88462:intron 20.0%
!!! TATGTTTTTCTATCAAAATG+TGG + chr8.3:57808167-57808186 MS.gene88462:intron 20.0%
!!! TCATTTTCTTAAATTATCAC+TGG + chr8.3:57807802-57807821 MS.gene88462:intron 20.0%
!!! TTTACATTTTTCACATTATC+TGG + chr8.3:57807649-57807668 MS.gene88462:intron 20.0%
! AAATTCACAAATCATGTCAA+AGG - chr8.3:57808544-57808563 None:intergenic 25.0%
! AAGAGCGATTATAATTCTAA+TGG + chr8.3:57809057-57809076 MS.gene88462:intron 25.0%
! AATTTACAAAATAGAGGGAA+AGG - chr8.3:57808857-57808876 None:intergenic 25.0%
! AGTGTTTAGAAACAAAATGA+AGG - chr8.3:57807510-57807529 None:intergenic 25.0%
! CAAGATAAACTCATCAAAAT+TGG + chr8.3:57811073-57811092 MS.gene88462:intron 25.0%
! GTACAAAATTGAATACTTGT+AGG + chr8.3:57809155-57809174 MS.gene88462:CDS 25.0%
! TAAAGATTCATGCTTACAAA+TGG - chr8.3:57808659-57808678 None:intergenic 25.0%
! TAATATCAACAAGTGTTACA+GGG - chr8.3:57809547-57809566 None:intergenic 25.0%
! TAATCGTCAAAACTTAATGT+GGG + chr8.3:57808765-57808784 MS.gene88462:intron 25.0%
! TAATGTTTCTTAGGTAGAAA+TGG + chr8.3:57809096-57809115 MS.gene88462:intron 25.0%
! TATTGGAAATATAGGATTAG+TGG - chr8.3:57808067-57808086 None:intergenic 25.0%
! TGGATTACTATTGGAAATAT+AGG - chr8.3:57808075-57808094 None:intergenic 25.0%
! TTAATCGTCAAAACTTAATG+TGG + chr8.3:57808764-57808783 MS.gene88462:intron 25.0%
! TTAGAAACAAAATGAAGGAA+AGG - chr8.3:57807505-57807524 None:intergenic 25.0%
! TTCTACCTAAGAAACATTAT+TGG - chr8.3:57809095-57809114 None:intergenic 25.0%
! TTGATAGAAAAACATATCTG+TGG - chr8.3:57808163-57808182 None:intergenic 25.0%
! TTGTAGTAAATTGTGTATCA+TGG + chr8.3:57808983-57809002 MS.gene88462:intron 25.0%
!! AACTACCATTTTCATGTTTA+CGG - chr8.3:57810472-57810491 None:intergenic 25.0%
!! ATCAATCACAATCAGTTTTA+AGG - chr8.3:57810563-57810582 None:intergenic 25.0%
!! GTTTTCAGAACTCTAAATAA+AGG - chr8.3:57810250-57810269 None:intergenic 25.0%
!! TTGAAGTTTGACTTTCTAAA+CGG - chr8.3:57808619-57808638 None:intergenic 25.0%
!!! ATTTAAGTAGCCTTATATAG+AGG - chr8.3:57809329-57809348 None:intergenic 25.0%
!!! CAAAAAGTTGTATTTTGAAC+TGG - chr8.3:57808268-57808287 None:intergenic 25.0%
!!! TAGGTAAAATGGATTACTAT+TGG - chr8.3:57808084-57808103 None:intergenic 25.0%
AAGAAACTGATGAACAATTC+AGG + chr8.3:57811115-57811134 MS.gene88462:CDS 30.0%
AATGAGATAAATCGGTAGTT+AGG - chr8.3:57809288-57809307 None:intergenic 30.0%
AGAGATAACCATGAAAAATC+CGG + chr8.3:57810982-57811001 MS.gene88462:CDS 30.0%
ATTGATGTGAGAGGTAATTA+AGG - chr8.3:57807455-57807474 None:intergenic 30.0%
CAACCTATGAAACATCAATA+CGG - chr8.3:57807869-57807888 None:intergenic 30.0%
CAATCTTTGTAAGCATGTAA+AGG + chr8.3:57810885-57810904 MS.gene88462:CDS 30.0%
CAATTACACTTACAAGAGTT+AGG - chr8.3:57808103-57808122 None:intergenic 30.0%
CTAATATCAACAAGTGTTAC+AGG - chr8.3:57809548-57809567 None:intergenic 30.0%
CTTACAAGAGTTAGGTAAAA+TGG - chr8.3:57808095-57808114 None:intergenic 30.0%
CTTTACATGCTTACAAAGAT+TGG - chr8.3:57810887-57810906 None:intergenic 30.0%
GGTGTTTCATTGTGATTATA+AGG + chr8.3:57810499-57810518 MS.gene88462:CDS 30.0%
GTTGACCAATAATGTTTCTT+AGG + chr8.3:57809087-57809106 MS.gene88462:intron 30.0%
TTAAACTCACTTGTCAATCA+CGG + chr8.3:57810612-57810631 MS.gene88462:intron 30.0%
! ACTTTGAGTTATCATGATGT+AGG - chr8.3:57808448-57808467 None:intergenic 30.0%
! AGAGGTAATTAAGGTCTTTT+TGG - chr8.3:57807446-57807465 None:intergenic 30.0%
! ATCTGAATAATTTTCGCCAT+CGG + chr8.3:57808703-57808722 MS.gene88462:intron 30.0%
! CTTTTCTCTTATTCCTTCTA+TGG - chr8.3:57807936-57807955 None:intergenic 30.0%
! TATTTTGTACTAGCATATCG+TGG + chr8.3:57810706-57810725 MS.gene88462:intron 30.0%
! TCTCTCTGAAAGTAATTGTT+AGG + chr8.3:57807899-57807918 MS.gene88462:intron 30.0%
! TGTGTATCATGGATTAATAG+TGG + chr8.3:57808994-57809013 MS.gene88462:intron 30.0%
! TTGTACTATTGATGATTGTC+AGG + chr8.3:57809902-57809921 MS.gene88462:CDS 30.0%
! TTTTCGACTTTGGTAAATCA+AGG + chr8.3:57807569-57807588 MS.gene88462:intron 30.0%
!! ATGAAAAGTACCTACTAATG+GGG + chr8.3:57807998-57808017 MS.gene88462:intron 30.0%
!! CTGAAGATACATATTTTTGC+AGG + chr8.3:57809580-57809599 MS.gene88462:intron 30.0%
!! CTGAATTTTAACATCAACAG+AGG - chr8.3:57810081-57810100 None:intergenic 30.0%
!! GATGAAAAGTACCTACTAAT+GGG + chr8.3:57807997-57808016 MS.gene88462:intron 30.0%
!! GTTGATTGATTTTTCGACTT+TGG + chr8.3:57807559-57807578 MS.gene88462:intron 30.0%
!! TACAAAGTGTTGTCAAATAG+CGG - chr8.3:57808968-57808987 None:intergenic 30.0%
!! TAGAGTTCTGAAAACATGTT+AGG + chr8.3:57810255-57810274 MS.gene88462:intron 30.0%
!! TGATGAAAAGTACCTACTAA+TGG + chr8.3:57807996-57808015 MS.gene88462:intron 30.0%
!!! CATTTTTTTACTTCTTCCAG+AGG + chr8.3:57809871-57809890 MS.gene88462:intron 30.0%
!!! CTTTTAATTTACCTCTCTTG+TGG + chr8.3:57810843-57810862 MS.gene88462:intron 30.0%
AAACATGACAAGACATGTCA+AGG + chr8.3:57810031-57810050 MS.gene88462:CDS 35.0%
AACAATTCAGGTCAAGGAAT+AGG + chr8.3:57811127-57811146 MS.gene88462:CDS 35.0%
ACAATTCAGGTCAAGGAATA+GGG + chr8.3:57811128-57811147 MS.gene88462:CDS 35.0%
AGGATTTCTTACACTTGAGA+AGG - chr8.3:57810537-57810556 None:intergenic 35.0%
AGTTAAGCTGGAATCAATAG+AGG + chr8.3:57810307-57810326 MS.gene88462:intron 35.0%
ATAAGCAACTCTAGTTGAGT+TGG - chr8.3:57811213-57811232 None:intergenic 35.0%
ATGATGAGAATTGATGTGAG+AGG - chr8.3:57807464-57807483 None:intergenic 35.0%
ATGTCATTGCATGTCTAAAC+TGG - chr8.3:57808293-57808312 None:intergenic 35.0%
CAACTAGAGTTGCTTATGAA+GGG + chr8.3:57811216-57811235 MS.gene88462:CDS 35.0%
CTAACCAACATTTCAACTGT+AGG - chr8.3:57811174-57811193 None:intergenic 35.0%
GTAGAAGATGACGATTCAAA+TGG - chr8.3:57809947-57809966 None:intergenic 35.0%
GTCATCTTCTACAACATGAT+GGG + chr8.3:57809955-57809974 MS.gene88462:CDS 35.0%
TAAAACTCTTCATACAGCCA+GGG - chr8.3:57810346-57810365 None:intergenic 35.0%
TAAGCAACTCTAGTTGAGTT+GGG - chr8.3:57811212-57811231 None:intergenic 35.0%
TAGGTAGAAATGGAAAAAGC+AGG + chr8.3:57809106-57809125 MS.gene88462:intron 35.0%
TAGTGGTATACTAGTCCTAA+TGG - chr8.3:57808050-57808069 None:intergenic 35.0%
TAGTGTGATACCTCATGATT+TGG + chr8.3:57808417-57808436 MS.gene88462:intron 35.0%
TATGCAAAGTCATTCACTTG+AGG + chr8.3:57809222-57809241 MS.gene88462:CDS 35.0%
TCAACTAGAGTTGCTTATGA+AGG + chr8.3:57811215-57811234 MS.gene88462:CDS 35.0%
TCACATTATCTGGTCATGTA+CGG + chr8.3:57807659-57807678 MS.gene88462:intron 35.0%
TCATCTTCTACAACATGATG+GGG + chr8.3:57809956-57809975 MS.gene88462:CDS 35.0%
TGAAAATGGTAGTTCATCAG+AGG + chr8.3:57810478-57810497 MS.gene88462:CDS 35.0%
TGAAGGGTTTATCTTGCAAA+TGG - chr8.3:57810915-57810934 None:intergenic 35.0%
TGTCAAGGAGTTTCATAACA+AGG + chr8.3:57810046-57810065 MS.gene88462:CDS 35.0%
TTCACAAATCATGTCAAAGG+TGG - chr8.3:57808541-57808560 None:intergenic 35.0%
TTGAATACTTGTAGGGAATG+TGG + chr8.3:57809163-57809182 MS.gene88462:CDS 35.0%
TTGAATCATAACCACAAGAG+AGG - chr8.3:57810857-57810876 None:intergenic 35.0%
TTGACTTTCTAAACGGTGTT+TGG - chr8.3:57808612-57808631 None:intergenic 35.0%
! AATTAAGGTCTTTTTGGACC+TGG - chr8.3:57807440-57807459 None:intergenic 35.0%
! CTACAGTTGAAATGTTGGTT+AGG + chr8.3:57811172-57811191 MS.gene88462:CDS 35.0%
! CTTTGGAAGACCTCTATATA+AGG + chr8.3:57809316-57809335 MS.gene88462:intron 35.0%
! GTAGGATTTTCCAAATCATG+AGG - chr8.3:57808430-57808449 None:intergenic 35.0%
! TACCATTTTCATGTTTACGG+AGG - chr8.3:57810469-57810488 None:intergenic 35.0%
! TATGTTTTCTGCAGTTAAGC+TGG + chr8.3:57810295-57810314 MS.gene88462:intron 35.0%
! TTCTGAAAACATGTTAGGCA+CGG + chr8.3:57810260-57810279 MS.gene88462:intron 35.0%
!! ATCAAAATTGGTTTGTGCAG+GGG + chr8.3:57811085-57811104 MS.gene88462:intron 35.0%
!! CATCAAAATTGGTTTGTGCA+GGG + chr8.3:57811084-57811103 MS.gene88462:intron 35.0%
!! TCATCAAAATTGGTTTGTGC+AGG + chr8.3:57811083-57811102 MS.gene88462:intron 35.0%
!!! CACAATCAGTTTTAAGGCTA+AGG - chr8.3:57810557-57810576 None:intergenic 35.0%
!!! CGCTATTTTTGTCAATACTG+TGG + chr8.3:57807409-57807428 MS.gene88462:CDS 35.0%
AAGTCTCACAGTTCACATAC+TGG - chr8.3:57810409-57810428 None:intergenic 40.0%
AATTCACGGTTCTCTAGCAA+GGG + chr8.3:57808492-57808511 MS.gene88462:intron 40.0%
ACAGGACATAGAAACTTACG+AGG - chr8.3:57811023-57811042 None:intergenic 40.0%
AGGATTTAGTGTGTGCTCTA+AGG - chr8.3:57810382-57810401 None:intergenic 40.0%
ATGACTTCCATCAGCAATGT+TGG - chr8.3:57807727-57807746 None:intergenic 40.0%
ATGTGAACTGTGAGACTTGT+GGG + chr8.3:57810411-57810430 MS.gene88462:CDS 40.0%
CAAGATAAACCCTTCAAGTG+CGG + chr8.3:57810919-57810938 MS.gene88462:CDS 40.0%
CAATTCAGGTCAAGGAATAG+GGG + chr8.3:57811129-57811148 MS.gene88462:CDS 40.0%
CACCTCCGTAAACATGAAAA+TGG + chr8.3:57810464-57810483 MS.gene88462:CDS 40.0%
CACTCATGGCTTATTCTTGT+TGG + chr8.3:57809797-57809816 MS.gene88462:intron 40.0%
CAGGACATAGAAACTTACGA+GGG - chr8.3:57811022-57811041 None:intergenic 40.0%
CATATTCTGCTACACTACAG+TGG + chr8.3:57808926-57808945 MS.gene88462:intron 40.0%
CCTATGAAACATCAATACGG+AGG - chr8.3:57807866-57807885 None:intergenic 40.0%
CGTCATCTTCTACAACATGA+TGG + chr8.3:57809954-57809973 MS.gene88462:CDS 40.0%
CTGATGAACAATTCAGGTCA+AGG + chr8.3:57811121-57811140 MS.gene88462:CDS 40.0%
GACTTTGCATATGCTGCAAT+AGG - chr8.3:57809213-57809232 None:intergenic 40.0%
GGAAACATGACATCCATAGA+AGG + chr8.3:57807920-57807939 MS.gene88462:intron 40.0%
GTAAAACTCTTCATACAGCC+AGG - chr8.3:57810347-57810366 None:intergenic 40.0%
GTGTCCTACAGTTGAAATGT+TGG + chr8.3:57811167-57811186 MS.gene88462:CDS 40.0%
TAGTACAAATATACGGCCTC+TGG - chr8.3:57809890-57809909 None:intergenic 40.0%
TATGTGAACTGTGAGACTTG+TGG + chr8.3:57810410-57810429 MS.gene88462:CDS 40.0%
TCAGGCAAGTTACAGAAGAA+AGG + chr8.3:57809920-57809939 MS.gene88462:CDS 40.0%
TTCCCTTCAATGAAAAGTCG+CGG - chr8.3:57810013-57810032 None:intergenic 40.0%
TTGCATATGCTGCAATAGGT+AGG - chr8.3:57809209-57809228 None:intergenic 40.0%
! AACCGCGACTTTTCATTGAA+GGG + chr8.3:57810008-57810027 MS.gene88462:CDS 40.0%
! AAGCAACTCTAGTTGAGTTG+GGG - chr8.3:57811211-57811230 None:intergenic 40.0%
! AAGGTTTTCATGTGGCAATC+AGG - chr8.3:57808216-57808235 None:intergenic 40.0%
! CCTCCGTATTGATGTTTCAT+AGG + chr8.3:57807863-57807882 MS.gene88462:intron 40.0%
! GAAAGAGATCAAGAAGCACA+GGG + chr8.3:57809133-57809152 MS.gene88462:CDS 40.0%
! GAGAAGCACAAAGATTCTCA+TGG + chr8.3:57810161-57810180 MS.gene88462:CDS 40.0%
! TCTTATGGTTAAGAGCGTGA+TGG + chr8.3:57808799-57808818 MS.gene88462:intron 40.0%
! TGAAAGAGATCAAGAAGCAC+AGG + chr8.3:57809132-57809151 MS.gene88462:CDS 40.0%
! TGACTTGATGGATCAGTATC+AGG + chr8.3:57809698-57809717 MS.gene88462:intron 40.0%
! TGATGGATCAGTATCAGGTA+GGG + chr8.3:57809703-57809722 MS.gene88462:intron 40.0%
! TTGATGGATCAGTATCAGGT+AGG + chr8.3:57809702-57809721 MS.gene88462:intron 40.0%
!! AAAAGTACCTACTAATGGGG+TGG + chr8.3:57808001-57808020 MS.gene88462:intron 40.0%
!! TCAAAATTGGTTTGTGCAGG+GGG + chr8.3:57811086-57811105 MS.gene88462:intron 40.0%
!!! TTGGCAGTTTTGTTCACTCA+TGG + chr8.3:57809783-57809802 MS.gene88462:intron 40.0%
AATCACGAAATGCCGAACAC+AGG + chr8.3:57807361-57807380 MS.gene88462:CDS 45.0%
ATATGCTGCAATAGGTAGGC+AGG - chr8.3:57809205-57809224 None:intergenic 45.0%
ATCACGAAATGCCGAACACA+GGG + chr8.3:57807362-57807381 MS.gene88462:CDS 45.0%
CAATTCACGGTTCTCTAGCA+AGG + chr8.3:57808491-57808510 MS.gene88462:intron 45.0%
CATTATCTGGTCATGTACGG+CGG + chr8.3:57807662-57807681 MS.gene88462:intron 45.0%
CCGCACATCGTGGAAAATAA+CGG + chr8.3:57808897-57808916 MS.gene88462:intron 45.0%
CTCTTGAGAAGCTCCATCAA+GGG + chr8.3:57809730-57809749 MS.gene88462:intron 45.0%
CTTGCTAGAGAACCGTGAAT+TGG - chr8.3:57808493-57808512 None:intergenic 45.0%
GAAGTTGTAGCGTGAAGCAT+TGG + chr8.3:57809640-57809659 MS.gene88462:intron 45.0%
GAATCATCCATCCCTGTGTT+CGG - chr8.3:57807376-57807395 None:intergenic 45.0%
GCAAATCTCATGCCACAATC+CGG - chr8.3:57810949-57810968 None:intergenic 45.0%
GCATTTCGTGATTGCTTCTG+CGG - chr8.3:57807354-57807373 None:intergenic 45.0%
GGTTCGCACAAACCAATTCA+CGG + chr8.3:57808478-57808497 MS.gene88462:intron 45.0%
GTCAGATATGACACTGACAC+TGG - chr8.3:57807844-57807863 None:intergenic 45.0%
TCGCGGTTGCAATTCTCAAT+TGG - chr8.3:57809996-57810015 None:intergenic 45.0%
TCTCACAGTTCACATACTGG+TGG - chr8.3:57810406-57810425 None:intergenic 45.0%
TGATCCAGTGTCGTATACTG+TGG + chr8.3:57809491-57809510 MS.gene88462:intron 45.0%
TGCAGTATTTGTGGCAGCTA+AGG - chr8.3:57808235-57808254 None:intergenic 45.0%
TGGGAGTTTGAGAGCTCTTA+TGG + chr8.3:57808784-57808803 MS.gene88462:intron 45.0%
! CAACCGCGACTTTTCATTGA+AGG + chr8.3:57810007-57810026 MS.gene88462:CDS 45.0%
! CATGCGAACCGGATTTTTCA+TGG - chr8.3:57810993-57811012 None:intergenic 45.0%
! TGGCAGCTAAGGTTTTCATG+TGG - chr8.3:57808224-57808243 None:intergenic 45.0%
! TTTGCTTCGAGTTCTTCCGA+TGG - chr8.3:57807331-57807350 None:intergenic 45.0%
!! ACTGCCAACCTCGTTTTGAA+CGG - chr8.3:57809771-57809790 None:intergenic 45.0%
!! AGCAACTCTAGTTGAGTTGG+GGG - chr8.3:57811210-57811229 None:intergenic 45.0%
!! ATCAGCAATGTTGGTGCTAC+CGG - chr8.3:57807718-57807737 None:intergenic 45.0%
!! CCGTTATTTTCCACGATGTG+CGG - chr8.3:57808900-57808919 None:intergenic 45.0%
!! GTGAATTGGTTTGTGCGAAC+CGG - chr8.3:57808479-57808498 None:intergenic 45.0%
!! TAACTCAAAGTACCGCGAAC+CGG + chr8.3:57808457-57808476 MS.gene88462:intron 45.0%
!!! GTGCTGTTTTCTCGGTCTTT+GGG + chr8.3:57808725-57808744 MS.gene88462:intron 45.0%
!!! TGCTGTTTTCTCGGTCTTTG+GGG + chr8.3:57808726-57808745 MS.gene88462:intron 45.0%
!! ATATTAAAATCATAAAATTA+TGG + chr8.3:57809618-57809637 MS.gene88462:intron 5.0%
ACAGTTCACATACTGGTGGC+AGG - chr8.3:57810402-57810421 None:intergenic 50.0%
CACAAATACTGCACGCACTG+AGG + chr8.3:57808242-57808261 MS.gene88462:intron 50.0%
CAGTCCACAGTATACGACAC+TGG - chr8.3:57809498-57809517 None:intergenic 50.0%
CCTCTTGAGAAGCTCCATCA+AGG + chr8.3:57809729-57809748 MS.gene88462:intron 50.0%
CCTTGATGGAGCTTCTCAAG+AGG - chr8.3:57809732-57809751 None:intergenic 50.0%
CTCAGACTCCAAAATGCGTG+AGG + chr8.3:57809665-57809684 MS.gene88462:intron 50.0%
CTCAGTGCGTGCAGTATTTG+TGG - chr8.3:57808244-57808263 None:intergenic 50.0%
CTTGCAACCACCCCATTAGT+AGG - chr8.3:57808011-57808030 None:intergenic 50.0%
GAGGGTAAAGACATGCGAAC+CGG - chr8.3:57811004-57811023 None:intergenic 50.0%
GAGGTAGTGTGCTTATACCC+TGG + chr8.3:57810326-57810345 MS.gene88462:CDS 50.0%
GGTAGCACCAACATTGCTGA+TGG + chr8.3:57807717-57807736 MS.gene88462:intron 50.0%
GTCAATACTGTGGCATCTCC+AGG + chr8.3:57807419-57807438 MS.gene88462:CDS 50.0%
TCAGGTCAAGGAATAGGGGA+GGG + chr8.3:57811133-57811152 MS.gene88462:CDS 50.0%
TTCAGGTCAAGGAATAGGGG+AGG + chr8.3:57811132-57811151 MS.gene88462:CDS 50.0%
! TTTTGAACGGAGCGTGACTG+AGG - chr8.3:57809758-57809777 None:intergenic 50.0%
!! GGCAGCATGGTTTGACTTGA+TGG + chr8.3:57809686-57809705 MS.gene88462:intron 50.0%
!!! GGTGCTGTTTTCTCGGTCTT+TGG + chr8.3:57808724-57808743 MS.gene88462:intron 50.0%
AAGTGCGGCTTTCCGGATTG+TGG + chr8.3:57810934-57810953 MS.gene88462:CDS 55.0%
ACCCTTCAAGTGCGGCTTTC+CGG + chr8.3:57810927-57810946 MS.gene88462:CDS 55.0%
ATCCGGAAAGCCGCACTTGA+AGG - chr8.3:57810932-57810951 None:intergenic 55.0%
CCAAAATGCGTGAGGCAGCA+TGG + chr8.3:57809673-57809692 MS.gene88462:intron 55.0%
CGAAATGCCGAACACAGGGA+TGG + chr8.3:57807366-57807385 MS.gene88462:CDS 55.0%
CGCTCCGTTCAAAACGAGGT+TGG + chr8.3:57809764-57809783 MS.gene88462:intron 55.0%
GTCACGCTCCGTTCAAAACG+AGG + chr8.3:57809760-57809779 MS.gene88462:intron 55.0%
TCCGGAAAGCCGCACTTGAA+GGG - chr8.3:57810931-57810950 None:intergenic 55.0%
! CCATGCTGCCTCACGCATTT+TGG - chr8.3:57809676-57809695 None:intergenic 55.0%
! GACCGAGAAAACAGCACCGA+TGG - chr8.3:57808722-57808741 None:intergenic 55.0%
!!! CGCCATCGGTGCTGTTTTCT+CGG + chr8.3:57808717-57808736 MS.gene88462:intron 55.0%
AGTGCTGTCACCGCACATCG+TGG + chr8.3:57808887-57808906 MS.gene88462:intron 60.0%
CCTTCCCACAGCCAGTTTCC+GGG - chr8.3:57810118-57810137 None:intergenic 60.0%
GCCTTCCCACAGCCAGTTTC+CGG - chr8.3:57810119-57810138 None:intergenic 60.0%
TCAGAAGCAGCACGTGTGCC+CGG + chr8.3:57810097-57810116 MS.gene88462:CDS 60.0%
TGTGCCCGGAAACTGGCTGT+GGG + chr8.3:57810111-57810130 MS.gene88462:CDS 60.0%
! CGTGACTGAGGCACCCTTGA+TGG - chr8.3:57809746-57809765 None:intergenic 60.0%
!! GGTTTGTGCGAACCGGTTCG+CGG - chr8.3:57808472-57808491 None:intergenic 60.0%
CAGCACGTGTGCCCGGAAAC+TGG + chr8.3:57810104-57810123 MS.gene88462:CDS 65.0%
CCCGGAAACTGGCTGTGGGA+AGG + chr8.3:57810115-57810134 MS.gene88462:CDS 65.0%
GTGTGCCCGGAAACTGGCTG+TGG + chr8.3:57810110-57810129 MS.gene88462:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 57807325 57811266 57807325 ID=MS.gene88462
chr8.3 mRNA 57807325 57811266 57807325 ID=MS.gene88462.t1;Parent=MS.gene88462
chr8.3 exon 57807325 57807486 57807325 ID=MS.gene88462.t1.exon1;Parent=MS.gene88462.t1
chr8.3 CDS 57807325 57807486 57807325 ID=cds.MS.gene88462.t1;Parent=MS.gene88462.t1
chr8.3 exon 57809109 57809243 57809109 ID=MS.gene88462.t1.exon2;Parent=MS.gene88462.t1
chr8.3 CDS 57809109 57809243 57809109 ID=cds.MS.gene88462.t1;Parent=MS.gene88462.t1
chr8.3 exon 57809891 57810182 57809891 ID=MS.gene88462.t1.exon3;Parent=MS.gene88462.t1
chr8.3 CDS 57809891 57810182 57809891 ID=cds.MS.gene88462.t1;Parent=MS.gene88462.t1
chr8.3 exon 57810309 57810544 57810309 ID=MS.gene88462.t1.exon4;Parent=MS.gene88462.t1
chr8.3 CDS 57810309 57810544 57810309 ID=cds.MS.gene88462.t1;Parent=MS.gene88462.t1
chr8.3 exon 57810880 57811023 57810880 ID=MS.gene88462.t1.exon5;Parent=MS.gene88462.t1
chr8.3 CDS 57810880 57811023 57810880 ID=cds.MS.gene88462.t1;Parent=MS.gene88462.t1
chr8.3 exon 57811105 57811266 57811105 ID=MS.gene88462.t1.exon6;Parent=MS.gene88462.t1
chr8.3 CDS 57811105 57811266 57811105 ID=cds.MS.gene88462.t1;Parent=MS.gene88462.t1
Gene Sequence

>MS.gene88462

ATGCCATCGGAAGAACTCGAAGCAAACCGCAGAAGCAATCACGAAATGCCGAACACAGGGATGGATGATTCATTTGAAAGTAGACGCTATTTTTGTCAATACTGTGGCATCTCCAGGTCCAAAAAGACCTTAATTACCTCTCACATCAATTCTCATCATCAAGTATATTTTTCTATTCCTTTCCTTCATTTTGTTTCTAAACACTTCGTCTGAATTGATAGATTTTCTAATTGAGTTGATTGATTTTTCGACTTTGGTAAATCAAGGCCAAAATAAAATACAAATTGCTGATTGCTTGAATGCAAATAATCAAAATCAAAATACTTTACATTTTTCACATTATCTGGTCATGTACGGCGGCGATATGTGTTTAAATATGATTTGATATCTCCGGTAGCACCAACATTGCTGATGGAAGTCATGCGTGTTTGATCGTGTCTGACACAACACTCACACATGCCATTACATCTAATTCATTCATTTTCTTAAATTATCACTGGTATTGACATGTCAATGCCAGTGTCAGTGTCATATCTGACCTCCGTATTGATGTTTCATAGGTTGACATACGATTTCTCTCTGAAAGTAATTGTTAGGAAACATGACATCCATAGAAGGAATAAGAGAAAAGTAATGAACTCTTTGTATTGATGAGAATAAAAAGTTACAAATGATGAAAAGTACCTACTAATGGGGTGGTTGCAAGTTATTTATACTATTATCCATTAGGACTAGTATACCACTAATCCTATATTTCCAATAGTAATCCATTTTACCTAACTCTTGTAAGTGTAATTGTATACAAATTTGTGGCAAATGTGCATACTCAATTTCTCCACAGATATGTTTTTCTATCAAAATGTGGTAATAATATGATGCAATGAAACACCTGATTGCCACATGAAAACCTTAGCTGCCACAAATACTGCACGCACTGAGGCCAGTTCAAAATACAACTTTTTGAACCAGTTTAGACATGCAATGACATTTGTATACACTGACATTTTTAAGAACTAATCTACACAAGTTATCGATTTTAGTAACTAAATTTTACATTGATTCATTTCAAAGTTTAGTTAATTTTGCGAGTGATAGTGTGATACCTCATGATTTGGAAAATCCTACATCATGATAACTCAAAGTACCGCGAACCGGTTCGCACAAACCAATTCACGGTTCTCTAGCAAGGGTAGCAAATTCTGTGACTATGCTACCACCTTTGACATGATTTGTGAATTTTTTCATTTCAAATGCATTTTTTTCAATTATGAAGTTTCAAATATTCCAAACACCGTTTAGAAAGTCAAACTTCAAATTCATGAAAAGAAAAACCATTTGTAAGCATGAATCTTTATATCTAGCTATAAGAGAGCTGCATATCTGAATAATTTTCGCCATCGGTGCTGTTTTCTCGGTCTTTGGGGTGATAGTTCTATTTATTAATCGTCAAAACTTAATGTGGGAGTTTGAGAGCTCTTATGGTTAAGAGCGTGATGGATAAACACACAAGACATTTTCTAACTTTTTGACCTTTCCCTCTATTTTGTAAATTCAGTATAATCAGTGCTGTCACCGCACATCGTGGAAAATAACGGTTTCTTCATATTCTGCTACACTACAGTGGTATAGCACTGCTACAGCCGCTATTTGACAACACTTTGTAGTAAATTGTGTATCATGGATTAATAGTGGTTTATTGAAATTTCACTACACTATGAGATCGCTATTTGACAAGAGCGATTATAATTCTAATGGTTATGTTGTTGACCAATAATGTTTCTTAGGTAGAAATGGAAAAAGCAGGACTTGAAAGAGATCAAGAAGCACAGGGTACAAAATTGAATACTTGTAGGGAATGTGGTGCGAGTTTTAAAAAACCTGCCTACCTATTGCAGCATATGCAAAGTCATTCACTTGAGGTATTTACTGACGATATAGTAAGATGACAGCATTCTAATGTCCTAACTACCGATTTATCTCATTATATATTTCTTTGGAAGACCTCTATATAAGGCTACTTAAATTGTGTTTTTTGAAATTCATTTCAATGGATTATGTTCCTTGAAATTTCTGTATAGATAAAAAAAAAAAAGGGAGATTATAATTAGTTCATGCTTTAGGTATCTTTAGATTTTGTTAAAATCTGGTTACATTTGTCTTTATGTTTGATCCAGTGTCGTATACTGTGGACTGTCTTAGTCAGCTCAATGAATTACGATCCCTGTAACACTTGTTGATATTAGTTTTTACATCAACTGAAGATACATATTTTTGCAGGAAAGTATATTCTATGATATTAAAATCATAAAATTATGGAAGTTGTAGCGTGAAGCATTGGATCTCAGACTCCAAAATGCGTGAGGCAGCATGGTTTGACTTGATGGATCAGTATCAGGTAGGGAGACCTCTTGAGAAGCTCCATCAAGGGTGCCTCAGTCACGCTCCGTTCAAAACGAGGTTGGCAGTTTTGTTCACTCATGGCTTATTCTTGTTGGAGCACATTTAATGCTTATTAAGTTATTAGTTTATGGTTTTCTAGCTTTTAACATTTTTTTACTTCTTCCAGAGGCCGTATATTTGTACTATTGATGATTGTCAGGCAAGTTACAGAAGAAAGGACCATTTGAATCGTCATCTTCTACAACATGATGGGGAAAAATTTAAATGTCCAATTGAGAATTGCAACCGCGACTTTTCATTGAAGGGAAACATGACAAGACATGTCAAGGAGTTTCATAACAAGGATTCTTCAACCTCTGTTGATGTTAAAATTCAGAAGCAGCACGTGTGCCCGGAAACTGGCTGTGGGAAGGCTTTCAAATTTGCATCAAAGCTAGAGAAGCACAAAGATTCTCATGGTAAACTTCATTTCAATATTTGATATTTATGAGCAAGTTCTTCATGTGTTGTACATAATTTGATCCTTTATTTAGAGTTCTGAAAACATGTTAGGCACGGTTTTGAAGAATGTATGTTTTCTGCAGTTAAGCTGGAATCAATAGAGGTAGTGTGCTTATACCCTGGCTGTATGAAGAGTTTTACTAATCTTCAGTGCCTTAGAGCACACACTAAATCCTGCCACCAGTATGTGAACTGTGAGACTTGTGGGAAAAAGCAACTGCGTAAGAACATTAAAAGACACCTCCGTAAACATGAAAATGGTAGTTCATCAGAGGTGTTTCATTGTGATTATAAGGATTGTAGCAGCACCTTCTCAAGTGTAAGAAATCCTTAGCCTTAAAACTGATTGTGATTGATGCACATTTTAATTAAAGACTTAGAATTTTTTAAACTCACTTGTCAATCACGGATTGTGAAAAATAGCAGTTTGTTTAAATTCAGCTGCATGACAGTGCTATAGTGCTGCTATAGATTTGACAATATTTTGTACTAGCATATCGTGGAACAATTGCGATCTGTTCAAATTTCGCTACACTATAGTGCTGCTGTAATCACTATTTAACAACATTGTTTTAACTACGGTTCAATTTAAAACATTTGTACATAAATTGTTCTTGCTTTTAATTTACCTCTCTTGTGGTTATGATTCAACAGAAATCCAATCTTTGTAAGCATGTAAAGGCTGTCCATTTGCAAGATAAACCCTTCAAGTGCGGCTTTCCGGATTGTGGCATGAGATTTGCTTATAAACATGTGAGAGATAACCATGAAAAATCCGGTTCGCATGTCTTTACCCTCGTAAGTTTCTATGTCCTGTAAAGAATCTGTTATATTATCTACATACATGCAAGATAAACTCATCAAAATTGGTTTGTGCAGGGGGATTTTGAAGAAACTGATGAACAATTCAGGTCAAGGAATAGGGGAGGGAGAAAGAGAAAGTGTCCTACAGTTGAAATGTTGGTTAGGAAGAGAGTTGCTCCCCCAACTCAACTAGAGTTGCTTATGAAGGGTGTAGAGTTTTTTAATTTTATGCAGTAA
Protein sequence

>MS.gene88462.t1

MPSEELEANRRSNHEMPNTGMDDSFESRRYFCQYCGISRSKKTLITSHINSHHQVEMEKAGLERDQEAQGTKLNTCRECGASFKKPAYLLQHMQSHSLERPYICTIDDCQASYRRKDHLNRHLLQHDGEKFKCPIENCNRDFSLKGNMTRHVKEFHNKDSSTSVDVKIQKQHVCPETGCGKAFKFASKLEKHKDSHVKLESIEVVCLYPGCMKSFTNLQCLRAHTKSCHQYVNCETCGKKQLRKNIKRHLRKHENGSSSEVFHCDYKDCSSTFSSKSNLCKHVKAVHLQDKPFKCGFPDCGMRFAYKHVRDNHEKSGSHVFTLGDFEETDEQFRSRNRGGRKRKCPTVEMLVRKRVAPPTQLELLMKGVEFFNFMQ