Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044331.t1 | XP_003601522.1 | 99.3 | 411 | 3 | 0 | 1 | 411 | 1 | 411 | 3.20E-198 | 701 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044331.t1 | Q9SSE5 | 55.6 | 423 | 125 | 10 | 1 | 411 | 1 | 372 | 3.0e-80 | 300.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044331.t1 | G7J8U1 | 99.3 | 411 | 3 | 0 | 1 | 411 | 1 | 411 | 2.3e-198 | 701.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene044331.t1 | TF | C2C2-CO-like |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene43742 | MS.gene044331 | PPI |
| MS.gene70471 | MS.gene044331 | PPI |
| MS.gene44846 | MS.gene044331 | PPI |
| MS.gene25698 | MS.gene044331 | PPI |
| MS.gene044331 | MS.gene009935 | PPI |
| MS.gene88720 | MS.gene044331 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044331.t1 | MTR_3g082630 | 99.270 | 411 | 3 | 0 | 1 | 411 | 1 | 411 | 0.0 | 846 |
| MS.gene044331.t1 | MTR_5g069480 | 68.916 | 415 | 118 | 7 | 1 | 410 | 1 | 409 | 0.0 | 514 |
| MS.gene044331.t1 | MTR_5g069480 | 65.926 | 270 | 85 | 5 | 1 | 265 | 1 | 268 | 1.17e-117 | 345 |
| MS.gene044331.t1 | MTR_2g088900 | 32.308 | 455 | 224 | 11 | 1 | 405 | 97 | 517 | 1.57e-62 | 210 |
| MS.gene044331.t1 | MTR_7g108150 | 48.544 | 103 | 53 | 0 | 5 | 107 | 20 | 122 | 2.39e-29 | 117 |
| MS.gene044331.t1 | MTR_7g108150 | 62.500 | 48 | 18 | 0 | 348 | 395 | 322 | 369 | 9.63e-12 | 66.6 |
| MS.gene044331.t1 | MTR_1g109350 | 44.860 | 107 | 57 | 1 | 1 | 107 | 1 | 105 | 1.34e-24 | 103 |
| MS.gene044331.t1 | MTR_7g083540 | 35.971 | 139 | 81 | 2 | 1 | 132 | 1 | 138 | 1.21e-21 | 96.3 |
| MS.gene044331.t1 | MTR_7g083540 | 56.061 | 66 | 29 | 0 | 331 | 396 | 318 | 383 | 1.75e-14 | 75.1 |
| MS.gene044331.t1 | MTR_1g013450 | 44.048 | 84 | 46 | 1 | 4 | 86 | 5 | 88 | 6.15e-17 | 81.6 |
| MS.gene044331.t1 | MTR_3g105710 | 39.216 | 102 | 61 | 1 | 4 | 104 | 5 | 106 | 5.48e-16 | 78.2 |
| MS.gene044331.t1 | MTR_3g105710 | 45.833 | 72 | 36 | 1 | 333 | 401 | 208 | 279 | 4.50e-11 | 63.5 |
| MS.gene044331.t1 | MTR_4g128930 | 38.889 | 90 | 54 | 1 | 4 | 92 | 21 | 110 | 5.26e-15 | 76.3 |
| MS.gene044331.t1 | MTR_7g018170 | 41.758 | 91 | 52 | 1 | 5 | 94 | 26 | 116 | 1.82e-14 | 75.1 |
| MS.gene044331.t1 | MTR_4g067320 | 38.542 | 96 | 49 | 2 | 1 | 86 | 1 | 96 | 1.22e-13 | 70.5 |
| MS.gene044331.t1 | MTR_4g067320 | 38.542 | 96 | 49 | 2 | 1 | 86 | 1 | 96 | 1.24e-13 | 70.5 |
| MS.gene044331.t1 | MTR_5g021580 | 38.095 | 84 | 44 | 1 | 1 | 76 | 1 | 84 | 2.54e-11 | 62.8 |
| MS.gene044331.t1 | MTR_5g021580 | 38.095 | 84 | 44 | 1 | 1 | 76 | 1 | 84 | 3.08e-11 | 62.8 |
| MS.gene044331.t1 | MTR_1g023260 | 34.021 | 97 | 53 | 2 | 1 | 86 | 1 | 97 | 4.00e-11 | 62.4 |
| MS.gene044331.t1 | MTR_1g023260 | 34.021 | 97 | 53 | 2 | 1 | 86 | 1 | 97 | 4.03e-11 | 63.5 |
| MS.gene044331.t1 | MTR_4g071200 | 35.052 | 97 | 52 | 2 | 1 | 86 | 1 | 97 | 5.64e-11 | 62.4 |
| MS.gene044331.t1 | MTR_3g113070 | 33.673 | 98 | 53 | 2 | 1 | 86 | 1 | 98 | 8.87e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044331.t1 | AT3G07650 | 52.955 | 423 | 136 | 10 | 1 | 411 | 1 | 372 | 9.05e-143 | 411 |
| MS.gene044331.t1 | AT3G07650 | 52.955 | 423 | 136 | 10 | 1 | 411 | 1 | 372 | 9.05e-143 | 411 |
| MS.gene044331.t1 | AT3G07650 | 52.955 | 423 | 136 | 10 | 1 | 411 | 1 | 372 | 9.05e-143 | 411 |
| MS.gene044331.t1 | AT3G07650 | 52.955 | 423 | 136 | 10 | 1 | 411 | 1 | 372 | 9.05e-143 | 411 |
| MS.gene044331.t1 | AT5G48250 | 53.699 | 419 | 140 | 10 | 1 | 411 | 1 | 373 | 1.32e-140 | 406 |
| MS.gene044331.t1 | AT3G21880 | 34.063 | 411 | 210 | 11 | 5 | 408 | 9 | 365 | 2.15e-61 | 203 |
| MS.gene044331.t1 | AT3G21880 | 34.063 | 411 | 210 | 11 | 5 | 408 | 9 | 365 | 2.15e-61 | 203 |
| MS.gene044331.t1 | AT3G21880 | 34.063 | 411 | 210 | 11 | 5 | 408 | 5 | 361 | 2.22e-61 | 202 |
| MS.gene044331.t1 | AT4G15250 | 53.153 | 111 | 50 | 2 | 5 | 115 | 5 | 113 | 6.74e-34 | 129 |
| MS.gene044331.t1 | AT4G15250 | 51.020 | 98 | 45 | 1 | 307 | 404 | 232 | 326 | 8.54e-20 | 90.1 |
| MS.gene044331.t1 | AT2G33500 | 47.170 | 106 | 55 | 1 | 5 | 109 | 12 | 117 | 2.81e-30 | 120 |
| MS.gene044331.t1 | AT2G33500 | 60.784 | 51 | 20 | 0 | 346 | 396 | 349 | 399 | 1.42e-12 | 69.3 |
| MS.gene044331.t1 | AT2G33500 | 47.170 | 106 | 55 | 1 | 5 | 109 | 12 | 117 | 2.93e-30 | 120 |
| MS.gene044331.t1 | AT2G33500 | 60.784 | 51 | 20 | 0 | 346 | 396 | 348 | 398 | 1.41e-12 | 69.3 |
| MS.gene044331.t1 | AT1G28050 | 39.041 | 146 | 86 | 2 | 5 | 147 | 9 | 154 | 5.53e-29 | 117 |
| MS.gene044331.t1 | AT1G28050 | 61.111 | 54 | 21 | 0 | 346 | 399 | 377 | 430 | 4.38e-13 | 70.9 |
| MS.gene044331.t1 | AT1G68190 | 46.847 | 111 | 57 | 2 | 1 | 110 | 10 | 119 | 5.74e-29 | 116 |
| MS.gene044331.t1 | AT1G68190 | 46.847 | 111 | 57 | 2 | 1 | 110 | 10 | 119 | 5.74e-29 | 116 |
| MS.gene044331.t1 | AT1G68190 | 46.847 | 111 | 57 | 2 | 1 | 110 | 39 | 148 | 1.01e-28 | 116 |
| MS.gene044331.t1 | AT2G47890 | 35.795 | 176 | 103 | 4 | 4 | 177 | 12 | 179 | 8.74e-26 | 105 |
| MS.gene044331.t1 | AT2G47890 | 36.420 | 162 | 95 | 3 | 4 | 165 | 12 | 165 | 3.16e-25 | 105 |
| MS.gene044331.t1 | AT2G47890 | 36.420 | 162 | 95 | 3 | 4 | 165 | 12 | 165 | 4.04e-25 | 105 |
| MS.gene044331.t1 | AT5G24930 | 43.333 | 90 | 50 | 1 | 4 | 92 | 5 | 94 | 4.07e-17 | 82.8 |
| MS.gene044331.t1 | AT5G24930 | 55.769 | 52 | 23 | 0 | 344 | 395 | 285 | 336 | 3.20e-11 | 64.7 |
| MS.gene044331.t1 | AT2G24790 | 42.222 | 90 | 51 | 1 | 4 | 92 | 7 | 96 | 8.55e-16 | 77.8 |
| MS.gene044331.t1 | AT2G24790 | 50.877 | 57 | 28 | 0 | 347 | 403 | 222 | 278 | 5.16e-11 | 63.5 |
| MS.gene044331.t1 | AT2G24790 | 42.222 | 90 | 51 | 1 | 4 | 92 | 7 | 96 | 8.64e-16 | 76.6 |
| MS.gene044331.t1 | AT5G15840 | 43.956 | 91 | 50 | 1 | 5 | 94 | 20 | 110 | 9.43e-15 | 75.9 |
| MS.gene044331.t1 | AT5G15840 | 43.956 | 91 | 50 | 1 | 5 | 94 | 20 | 110 | 1.59e-14 | 73.9 |
| MS.gene044331.t1 | AT5G15850 | 44.186 | 86 | 47 | 1 | 5 | 89 | 12 | 97 | 2.47e-14 | 74.3 |
| MS.gene044331.t1 | AT3G02380 | 40.000 | 100 | 53 | 2 | 5 | 97 | 16 | 115 | 1.43e-13 | 72.0 |
| MS.gene044331.t1 | AT3G02380 | 62.500 | 48 | 18 | 0 | 348 | 395 | 272 | 319 | 5.23e-12 | 67.0 |
| MS.gene044331.t1 | AT5G57660 | 30.216 | 139 | 92 | 2 | 5 | 142 | 22 | 156 | 2.33e-12 | 68.2 |
| MS.gene044331.t1 | AT2G31380 | 36.458 | 96 | 51 | 2 | 1 | 86 | 1 | 96 | 2.43e-12 | 66.6 |
| MS.gene044331.t1 | AT1G06040 | 37.500 | 96 | 50 | 2 | 1 | 86 | 1 | 96 | 3.90e-12 | 65.1 |
| MS.gene044331.t1 | AT1G06040 | 37.500 | 96 | 50 | 2 | 1 | 86 | 1 | 96 | 4.25e-12 | 66.2 |
| MS.gene044331.t1 | AT2G21320 | 39.286 | 84 | 43 | 1 | 1 | 76 | 1 | 84 | 5.78e-11 | 61.6 |
| MS.gene044331.t1 | AT4G38960 | 36.905 | 84 | 45 | 1 | 1 | 76 | 1 | 84 | 6.07e-11 | 61.6 |
| MS.gene044331.t1 | AT4G38960 | 36.905 | 84 | 45 | 1 | 1 | 76 | 1 | 84 | 6.07e-11 | 61.6 |
| MS.gene044331.t1 | AT1G75540 | 34.343 | 99 | 52 | 2 | 1 | 86 | 1 | 99 | 8.81e-11 | 63.2 |
Find 92 sgRNAs with CRISPR-Local
Find 144 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTGGATTTAACAGTTTATT+TGG | 0.095234 | 3.4:-77831040 | MS.gene044331:CDS |
| GTGGATTTAACAGTTTATTT+GGG | 0.284402 | 3.4:-77831039 | MS.gene044331:CDS |
| CGCGGTTCTTTGCTTGATTC+GGG | 0.298118 | 3.4:+77831485 | None:intergenic |
| AGCTACTGATCTACCTGATT+TGG | 0.310422 | 3.4:-77831537 | MS.gene044331:CDS |
| TCATCATCATCACTTGCTTC+AGG | 0.322324 | 3.4:+77831169 | None:intergenic |
| ACGCGGTTCTTTGCTTGATT+CGG | 0.327280 | 3.4:+77831484 | None:intergenic |
| CGGCCGAACTTTCTTCGATA+TGG | 0.346888 | 3.4:-77831688 | MS.gene044331:CDS |
| GGATGAAATGGATATAAATA+TGG | 0.347846 | 3.4:-77831122 | MS.gene044331:CDS |
| CGATTTCGATATGGATGAAA+TGG | 0.355447 | 3.4:-77831134 | MS.gene044331:CDS |
| TACCTTTGGTACACATTCAT+TGG | 0.361210 | 3.4:+77831436 | None:intergenic |
| GATGCTGCAGTGCTGCATGC+TGG | 0.368884 | 3.4:+77830538 | None:intergenic |
| TATGTTCAGGGTTCATCAAT+TGG | 0.370264 | 3.4:-77830580 | MS.gene044331:intron |
| ATTATACACTCAGTAGCTTC+TGG | 0.377897 | 3.4:+77829773 | None:intergenic |
| ACCGGCCCTTCACGCGCCTT+CGG | 0.385614 | 3.4:+77829841 | None:intergenic |
| GATCGTTTGCCTCTTATGAT+TGG | 0.395417 | 3.4:+77831736 | None:intergenic |
| CACTTTGTCAGAATTGTGAT+TGG | 0.398736 | 3.4:-77831784 | MS.gene044331:CDS |
| TTTGCTTGATTCGGGCTTTG+AGG | 0.401934 | 3.4:+77831493 | None:intergenic |
| ATGCAGTTCACAACCTGCTT+TGG | 0.404101 | 3.4:-77831831 | MS.gene044331:CDS |
| GTTCACAACCTGCTTTGGTT+AGG | 0.412214 | 3.4:-77831826 | MS.gene044331:CDS |
| TAGCTTCTGGTTTGGCTCAA+AGG | 0.415587 | 3.4:+77829786 | None:intergenic |
| ACACTCAGTAGCTTCTGGTT+TGG | 0.416653 | 3.4:+77829778 | None:intergenic |
| CATTCATTGGTAGATCCATC+TGG | 0.421154 | 3.4:+77831449 | None:intergenic |
| AGCTTCTTCAATGCTTCTCA+TGG | 0.422785 | 3.4:-77830389 | MS.gene044331:CDS |
| AGCTTCTGGTTTGGCTCAAA+GGG | 0.425533 | 3.4:+77829787 | None:intergenic |
| CTACATCTTCCAATCATAAG+AGG | 0.437225 | 3.4:-77831745 | MS.gene044331:CDS |
| CAGAAAAGGAAAGGCTCGAT+TGG | 0.437549 | 3.4:+77830455 | None:intergenic |
| GCTCAAAGGGTCATAATCAT+AGG | 0.437780 | 3.4:+77829800 | None:intergenic |
| AGCATTAGCATCTTGACAAT+TGG | 0.444014 | 3.4:+77830991 | None:intergenic |
| TGTCAGAATTGTGATTGGTT+AGG | 0.444655 | 3.4:-77831779 | MS.gene044331:CDS |
| GCGAAAGACTTATCCAAATC+AGG | 0.451210 | 3.4:+77831524 | None:intergenic |
| TGTAGTTGTAGCTCATTCTC+AGG | 0.468722 | 3.4:+77830340 | None:intergenic |
| CATCATCATCACTTGCTTCA+GGG | 0.475636 | 3.4:+77831170 | None:intergenic |
| TCCGAAGGCGCGTGAAGGGC+CGG | 0.483385 | 3.4:-77829842 | MS.gene044331:CDS |
| TTTGCAGTAATACAAAGATT+CGG | 0.484517 | 3.4:+77830484 | None:intergenic |
| GGGGCAAAAGCCATGTCTGC+TGG | 0.484909 | 3.4:-77831019 | MS.gene044331:CDS |
| TAATGAGAATAGGAGTGCTT+GGG | 0.485947 | 3.4:-77831597 | MS.gene044331:CDS |
| TTCTCATGGGAGAACCTCCT+TGG | 0.487490 | 3.4:-77830375 | MS.gene044331:CDS |
| TCATGAGTATTAATGAGAAT+AGG | 0.491713 | 3.4:-77831607 | MS.gene044331:CDS |
| TCTGTATGGCGATTTCGATA+TGG | 0.492638 | 3.4:-77831143 | MS.gene044331:CDS |
| AGACCATATCGAAGAAAGTT+CGG | 0.494435 | 3.4:+77831685 | None:intergenic |
| GCTTCAGGGCAATTTGTAGC+AGG | 0.495109 | 3.4:+77831184 | None:intergenic |
| AGAAAAGGAAAGGCTCGATT+GGG | 0.501130 | 3.4:+77830456 | None:intergenic |
| TTAATGAGAATAGGAGTGCT+TGG | 0.501567 | 3.4:-77831598 | MS.gene044331:CDS |
| ATTAATGAAGACGGTGGTGC+TGG | 0.505210 | 3.4:-77830430 | MS.gene044331:CDS |
| GTCTTCATTAATACCAGAAA+AGG | 0.513355 | 3.4:+77830441 | None:intergenic |
| ATATGCCTCTCGCAAGGAAA+GGG | 0.514733 | 3.4:-77829871 | MS.gene044331:CDS |
| CCCTTGCTCGAAGACATTCA+AGG | 0.516832 | 3.4:-77831871 | MS.gene044331:CDS |
| TGATGTCCGAAGGCGCGTGA+AGG | 0.519956 | 3.4:-77829847 | MS.gene044331:CDS |
| GTCATAATCATAGGCCTCGC+CGG | 0.520189 | 3.4:+77829809 | None:intergenic |
| CAAACGATCAATTGCTACTC+AGG | 0.524349 | 3.4:-77831722 | MS.gene044331:CDS |
| CCTCGCCGGCTTTAACAAAC+CGG | 0.526175 | 3.4:+77829823 | None:intergenic |
| ACTACATGTGAGCAAGAACT+AGG | 0.526382 | 3.4:-77831632 | MS.gene044331:CDS |
| TTGTGGAGATCAACGGTCCA+TGG | 0.528039 | 3.4:-77831960 | MS.gene044331:CDS |
| TCCTTGAATGTCTTCGAGCA+AGG | 0.533062 | 3.4:+77831870 | None:intergenic |
| TTCGGGCTTTGAGGATGTAC+CGG | 0.533096 | 3.4:+77831502 | None:intergenic |
| GATGTCCGAAGGCGCGTGAA+GGG | 0.533693 | 3.4:-77829846 | MS.gene044331:CDS |
| AAGGGCCGGTTTGTTAAAGC+CGG | 0.538044 | 3.4:-77829828 | MS.gene044331:CDS |
| CGTATGCTCGACCGCCCAGA+TGG | 0.548257 | 3.4:-77831464 | MS.gene044331:CDS |
| AATTGTGATTGGTTAGGTCA+TGG | 0.558365 | 3.4:-77831773 | MS.gene044331:CDS |
| CTACTCAGGTTGCCCTTCGT+CGG | 0.563208 | 3.4:-77831708 | MS.gene044331:CDS |
| GCTTCTTCAATGCTTCTCAT+GGG | 0.566506 | 3.4:-77830388 | MS.gene044331:CDS |
| TCAGAACACCTAACCAAAGC+AGG | 0.567607 | 3.4:+77831818 | None:intergenic |
| ATTGGAATCTCCAGCAGACA+TGG | 0.567887 | 3.4:+77831009 | None:intergenic |
| AAGAAAGTTCGGCCGACGAA+GGG | 0.569995 | 3.4:+77831696 | None:intergenic |
| CATGTAGTTTCACTCAGAGA+AGG | 0.576596 | 3.4:+77831647 | None:intergenic |
| CAAACATAGACACGCTGCAT+CGG | 0.578296 | 3.4:+77831925 | None:intergenic |
| TAGTGCTGTTATGCGATACA+AGG | 0.579883 | 3.4:-77830302 | MS.gene044331:CDS |
| TACCAATGAATGTGTACCAA+AGG | 0.580738 | 3.4:-77831438 | MS.gene044331:intron |
| CCTTGAATGTCTTCGAGCAA+GGG | 0.583830 | 3.4:+77831871 | None:intergenic |
| GATATGCCTCTCGCAAGGAA+AGG | 0.583865 | 3.4:-77829872 | MS.gene044331:CDS |
| TGGATTTAACAGTTTATTTG+GGG | 0.588596 | 3.4:-77831038 | MS.gene044331:CDS |
| GATTTGGATAAGTCTTTCGC+CGG | 0.589605 | 3.4:-77831521 | MS.gene044331:CDS |
| CATTAATACCAGAAAAGGAA+AGG | 0.592524 | 3.4:+77830446 | None:intergenic |
| GAAGAAAGTTCGGCCGACGA+AGG | 0.593920 | 3.4:+77831695 | None:intergenic |
| AGGAAAGGGCTGATGTCCGA+AGG | 0.595717 | 3.4:-77829857 | MS.gene044331:CDS |
| ATGTAGTTTCACTCAGAGAA+GGG | 0.599442 | 3.4:+77831648 | None:intergenic |
| ATCAGCCCTTTCCTTGCGAG+AGG | 0.604664 | 3.4:+77829866 | None:intergenic |
| ATTCATTGGTAGATCCATCT+GGG | 0.610627 | 3.4:+77831450 | None:intergenic |
| AAAGCTCTTCAGAATGAGTG+AGG | 0.617795 | 3.4:+77831071 | None:intergenic |
| GCTGGAGACTATCAAGATTG+TGG | 0.619737 | 3.4:-77830412 | MS.gene044331:CDS |
| AGTGAGATATGCCTCTCGCA+AGG | 0.625277 | 3.4:-77829877 | MS.gene044331:CDS |
| TCTGGTATTAATGAAGACGG+TGG | 0.629157 | 3.4:-77830436 | MS.gene044331:CDS |
| CCGGTTTGTTAAAGCCGGCG+AGG | 0.638136 | 3.4:-77829823 | MS.gene044331:CDS |
| TCAAGATGCTAATGCTGCTG+AGG | 0.640291 | 3.4:-77830984 | MS.gene044331:intron |
| TCTGGGCGGTCGAGCATACG+CGG | 0.641340 | 3.4:+77831467 | None:intergenic |
| TCAGGACAAGTGTTTAACCA+AGG | 0.666255 | 3.4:+77830358 | None:intergenic |
| AAGCTCTTCAGAATGAGTGA+GGG | 0.667176 | 3.4:+77831072 | None:intergenic |
| GGACAAGTGTTTAACCAAGG+AGG | 0.698616 | 3.4:+77830361 | None:intergenic |
| ATCGGAGCGGCAGTACACCA+TGG | 0.702100 | 3.4:+77831943 | None:intergenic |
| ATCGCATAACAGCACTACAG+CGG | 0.703445 | 3.4:+77830308 | None:intergenic |
| ATAGACACGCTGCATCGGAG+CGG | 0.713055 | 3.4:+77831930 | None:intergenic |
| CATTGGTAGATCCATCTGGG+CGG | 0.761162 | 3.4:+77831453 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTAATTTGATAAAAATGTA+AGG | + | chr3.4:77830522-77830541 | None:intergenic | 15.0% |
| !!! | ATTGATTGTTATTATCTTTT+AGG | - | chr3.4:77831799-77831818 | MS.gene044331:CDS | 15.0% |
| !!! | AATAAGTTGAAAACAACTTA+TGG | + | chr3.4:77830945-77830964 | None:intergenic | 20.0% |
| !!! | ACATCTAATTTTTATGTTCA+GGG | - | chr3.4:77831170-77831189 | MS.gene044331:CDS | 20.0% |
| !!! | TACATCTAATTTTTATGTTC+AGG | - | chr3.4:77831169-77831188 | MS.gene044331:CDS | 20.0% |
| ! | AACATTCAGATATATACCTT+TGG | + | chr3.4:77830343-77830362 | None:intergenic | 25.0% |
| ! | ACTTTCCCTTAAAAGATATA+AGG | - | chr3.4:77831615-77831634 | MS.gene044331:CDS | 25.0% |
| ! | ATACAAATTGTTTACACCTT+CGG | - | chr3.4:77831119-77831138 | MS.gene044331:CDS | 25.0% |
| ! | GGATGAAATGGATATAAATA+TGG | - | chr3.4:77830640-77830659 | MS.gene044331:intron | 25.0% |
| ! | GGTAACATGATAATCAAATT+AGG | + | chr3.4:77831699-77831718 | None:intergenic | 25.0% |
| ! | GTGGATTTAACAGTTTATTT+GGG | - | chr3.4:77830723-77830742 | MS.gene044331:intron | 25.0% |
| ! | TCATGAGTATTAATGAGAAT+AGG | - | chr3.4:77830155-77830174 | MS.gene044331:intron | 25.0% |
| ! | TGGATTTAACAGTTTATTTG+GGG | - | chr3.4:77830724-77830743 | MS.gene044331:intron | 25.0% |
| ! | TTATATATTAGCATAAGCAC+TGG | - | chr3.4:77830876-77830895 | MS.gene044331:intron | 25.0% |
| ! | TTTGCAGTAATACAAAGATT+CGG | + | chr3.4:77831281-77831300 | None:intergenic | 25.0% |
| !! | TCAGACCTTATATCTTTTAA+GGG | + | chr3.4:77831623-77831642 | None:intergenic | 25.0% |
| !! | TTCAGACCTTATATCTTTTA+AGG | + | chr3.4:77831624-77831643 | None:intergenic | 25.0% |
| !!! | TTTTCTGGTATTAATGAAGA+CGG | - | chr3.4:77831323-77831342 | MS.gene044331:intron | 25.0% |
| ACGCAATCAAATGATAGAAA+AGG | + | chr3.4:77830842-77830861 | None:intergenic | 30.0% | |
| AGAGGAAAAACACATTCAAT+AGG | + | chr3.4:77831558-77831577 | None:intergenic | 30.0% | |
| ATGATTAAGCTATTGCTCTA+TGG | + | chr3.4:77830417-77830436 | None:intergenic | 30.0% | |
| CATTAATACCAGAAAAGGAA+AGG | + | chr3.4:77831319-77831338 | None:intergenic | 30.0% | |
| GGATTTCAATTATAAACCTC+AGG | + | chr3.4:77830501-77830520 | None:intergenic | 30.0% | |
| GTCTTCATTAATACCAGAAA+AGG | + | chr3.4:77831324-77831343 | None:intergenic | 30.0% | |
| TAGAGCAATAGCTTAATCAT+TGG | - | chr3.4:77830417-77830436 | MS.gene044331:CDS | 30.0% | |
| TGAAAAGAGTTTCTAAACAG+TGG | - | chr3.4:77831640-77831659 | MS.gene044331:CDS | 30.0% | |
| TGCTAACAAGTTAGGAATTA+CGG | - | chr3.4:77831724-77831743 | MS.gene044331:CDS | 30.0% | |
| TTTCTATCATTTGATTGCGT+TGG | - | chr3.4:77830842-77830861 | MS.gene044331:intron | 30.0% | |
| ! | CTGTTTTCATAAGCTTATCT+TGG | + | chr3.4:77830980-77830999 | None:intergenic | 30.0% |
| ! | GAGAACTATGATGAACTTTT+TGG | - | chr3.4:77830662-77830681 | MS.gene044331:intron | 30.0% |
| ! | GCGTTTTTCATTCAAAAAAG+AGG | + | chr3.4:77831576-77831595 | None:intergenic | 30.0% |
| ! | GGTGGATTTAACAGTTTATT+TGG | - | chr3.4:77830722-77830741 | MS.gene044331:intron | 30.0% |
| ! | GGTTACATATGTGATTTTTG+TGG | - | chr3.4:77829785-77829804 | MS.gene044331:CDS | 30.0% |
| !! | CTATGATGAACTTTTTGGTA+TGG | - | chr3.4:77830667-77830686 | MS.gene044331:intron | 30.0% |
| !! | TCTGAAGAGCTTTTTGAAAA+TGG | - | chr3.4:77830701-77830720 | MS.gene044331:intron | 30.0% |
| !! | TTCAGTTTTAGTGCTCAATA+TGG | + | chr3.4:77831258-77831277 | None:intergenic | 30.0% |
| ACAGAGCTTATGTCTACTAT+TGG | - | chr3.4:77831081-77831100 | MS.gene044331:CDS | 35.0% | |
| AGACCATATCGAAGAAAGTT+CGG | + | chr3.4:77830080-77830099 | None:intergenic | 35.0% | |
| AGCATTAGCATCTTGACAAT+TGG | + | chr3.4:77830774-77830793 | None:intergenic | 35.0% | |
| AGCTTATCTTGGAGAACTTA+TGG | + | chr3.4:77830969-77830988 | None:intergenic | 35.0% | |
| ATGTAGTTTCACTCAGAGAA+GGG | + | chr3.4:77830117-77830136 | None:intergenic | 35.0% | |
| ATTCATTGGTAGATCCATCT+GGG | + | chr3.4:77830315-77830334 | None:intergenic | 35.0% | |
| CACTTTGTCAGAATTGTGAT+TGG | - | chr3.4:77829978-77829997 | MS.gene044331:intron | 35.0% | |
| CGATTTCGATATGGATGAAA+TGG | - | chr3.4:77830628-77830647 | MS.gene044331:intron | 35.0% | |
| CTACATCTTCCAATCATAAG+AGG | - | chr3.4:77830017-77830036 | MS.gene044331:intron | 35.0% | |
| CTCTACTTAAAATCAACCGA+AGG | + | chr3.4:77831138-77831157 | None:intergenic | 35.0% | |
| GAATGAAAAACGCCTAACAA+GGG | - | chr3.4:77831583-77831602 | MS.gene044331:CDS | 35.0% | |
| GCATTACTCACTTTACAAAC+TGG | - | chr3.4:77831662-77831681 | MS.gene044331:CDS | 35.0% | |
| GCTAACAAGTTAGGAATTAC+GGG | - | chr3.4:77831725-77831744 | MS.gene044331:CDS | 35.0% | |
| TACCAATGAATGTGTACCAA+AGG | - | chr3.4:77830324-77830343 | MS.gene044331:CDS | 35.0% | |
| TACCTTTGGTACACATTCAT+TGG | + | chr3.4:77830329-77830348 | None:intergenic | 35.0% | |
| TAGTAGACATAAGCTCTGTT+TGG | + | chr3.4:77831080-77831099 | None:intergenic | 35.0% | |
| TATGTTCAGGGTTCATCAAT+TGG | - | chr3.4:77831182-77831201 | MS.gene044331:CDS | 35.0% | |
| TGAATGAAAAACGCCTAACA+AGG | - | chr3.4:77831582-77831601 | MS.gene044331:CDS | 35.0% | |
| ! | AATTGTGATTGGTTAGGTCA+TGG | - | chr3.4:77829989-77830008 | MS.gene044331:intron | 35.0% |
| ! | GATGATGATGATGATCTGTA+TGG | - | chr3.4:77830605-77830624 | MS.gene044331:intron | 35.0% |
| ! | GTGATTTTTGTGGAGATCAA+CGG | - | chr3.4:77829795-77829814 | MS.gene044331:CDS | 35.0% |
| ! | TCAGACACGTTCTGATTTTT+CGG | + | chr3.4:77830196-77830215 | None:intergenic | 35.0% |
| ! | TGTCAGAATTGTGATTGGTT+AGG | - | chr3.4:77829983-77830002 | MS.gene044331:intron | 35.0% |
| !! | AACTCTCATTGTTGATTTCC+AGG | - | chr3.4:77830547-77830566 | MS.gene044331:CDS | 35.0% |
| !! | GAAGAGCTTTTTGAAAATGG+TGG | - | chr3.4:77830704-77830723 | MS.gene044331:intron | 35.0% |
| !! | TAATGAGAATAGGAGTGCTT+GGG | - | chr3.4:77830165-77830184 | MS.gene044331:intron | 35.0% |
| !! | TCCTAACTTGTTAGCAAAGA+CGG | + | chr3.4:77831720-77831739 | None:intergenic | 35.0% |
| !! | TTAATGAGAATAGGAGTGCT+TGG | - | chr3.4:77830164-77830183 | MS.gene044331:intron | 35.0% |
| AAAGCTCTTCAGAATGAGTG+AGG | + | chr3.4:77830694-77830713 | None:intergenic | 40.0% | |
| AAGCTCTTCAGAATGAGTGA+GGG | + | chr3.4:77830693-77830712 | None:intergenic | 40.0% | |
| ACCGTCTTTGCTAACAAGTT+AGG | - | chr3.4:77831716-77831735 | MS.gene044331:CDS | 40.0% | |
| ACTACATGTGAGCAAGAACT+AGG | - | chr3.4:77830130-77830149 | MS.gene044331:intron | 40.0% | |
| AGAAAAGGAAAGGCTCGATT+GGG | + | chr3.4:77831309-77831328 | None:intergenic | 40.0% | |
| AGCTACTGATCTACCTGATT+TGG | - | chr3.4:77830225-77830244 | MS.gene044331:intron | 40.0% | |
| AGCTTCTTCAATGCTTCTCA+TGG | - | chr3.4:77831373-77831392 | MS.gene044331:intron | 40.0% | |
| CAAACGATCAATTGCTACTC+AGG | - | chr3.4:77830040-77830059 | MS.gene044331:intron | 40.0% | |
| CATCATCATCACTTGCTTCA+GGG | + | chr3.4:77830595-77830614 | None:intergenic | 40.0% | |
| CATGTAGTTTCACTCAGAGA+AGG | + | chr3.4:77830118-77830137 | None:intergenic | 40.0% | |
| CATTCATTGGTAGATCCATC+TGG | + | chr3.4:77830316-77830335 | None:intergenic | 40.0% | |
| GATCGTTTGCCTCTTATGAT+TGG | + | chr3.4:77830029-77830048 | None:intergenic | 40.0% | |
| GATTTGGATAAGTCTTTCGC+CGG | - | chr3.4:77830241-77830260 | MS.gene044331:intron | 40.0% | |
| GCGAAAGACTTATCCAAATC+AGG | + | chr3.4:77830241-77830260 | None:intergenic | 40.0% | |
| GCTCAAAGGGTCATAATCAT+AGG | + | chr3.4:77831965-77831984 | None:intergenic | 40.0% | |
| GCTTCTTCAATGCTTCTCAT+GGG | - | chr3.4:77831374-77831393 | MS.gene044331:intron | 40.0% | |
| TAAACGAGTTTCACTACCTG+AGG | - | chr3.4:77830482-77830501 | MS.gene044331:CDS | 40.0% | |
| TCAGGACAAGTGTTTAACCA+AGG | + | chr3.4:77831407-77831426 | None:intergenic | 40.0% | |
| TCATCATCATCACTTGCTTC+AGG | + | chr3.4:77830596-77830615 | None:intergenic | 40.0% | |
| TCTGGTATTAATGAAGACGG+TGG | - | chr3.4:77831326-77831345 | MS.gene044331:intron | 40.0% | |
| TCTGTATGGCGATTTCGATA+TGG | - | chr3.4:77830619-77830638 | MS.gene044331:intron | 40.0% | |
| TGTAGTTGTAGCTCATTCTC+AGG | + | chr3.4:77831425-77831444 | None:intergenic | 40.0% | |
| ! | TAGTGCTGTTATGCGATACA+AGG | - | chr3.4:77831460-77831479 | MS.gene044331:CDS | 40.0% |
| AAAAACGCCTAACAAGGGTG+GGG | - | chr3.4:77831588-77831607 | MS.gene044331:CDS | 45.0% | |
| ACGCGGTTCTTTGCTTGATT+CGG | + | chr3.4:77830281-77830300 | None:intergenic | 45.0% | |
| AGACTGTTTGGGAGAGCTTA+TGG | - | chr3.4:77830901-77830920 | MS.gene044331:intron | 45.0% | |
| AGCTTAATCATTGGCGAAGC+AGG | - | chr3.4:77830426-77830445 | MS.gene044331:CDS | 45.0% | |
| ATATGCCTCTCGCAAGGAAA+GGG | - | chr3.4:77831891-77831910 | MS.gene044331:CDS | 45.0% | |
| ATCGCATAACAGCACTACAG+CGG | + | chr3.4:77831457-77831476 | None:intergenic | 45.0% | |
| ATGCAGTTCACAACCTGCTT+TGG | - | chr3.4:77829931-77829950 | MS.gene044331:intron | 45.0% | |
| ATTAATGAAGACGGTGGTGC+TGG | - | chr3.4:77831332-77831351 | MS.gene044331:intron | 45.0% | |
| ATTGGAATCTCCAGCAGACA+TGG | + | chr3.4:77830756-77830775 | None:intergenic | 45.0% | |
| CAAACATAGACACGCTGCAT+CGG | + | chr3.4:77829840-77829859 | None:intergenic | 45.0% | |
| CAGAAAAGGAAAGGCTCGAT+TGG | + | chr3.4:77831310-77831329 | None:intergenic | 45.0% | |
| CCTTGAATGTCTTCGAGCAA+GGG | + | chr3.4:77829894-77829913 | None:intergenic | 45.0% | |
| GAAAAACGCCTAACAAGGGT+GGG | - | chr3.4:77831587-77831606 | MS.gene044331:CDS | 45.0% | |
| GCTGGAGACTATCAAGATTG+TGG | - | chr3.4:77831350-77831369 | MS.gene044331:intron | 45.0% | |
| GGACAAGTGTTTAACCAAGG+AGG | + | chr3.4:77831404-77831423 | None:intergenic | 45.0% | |
| GTTCACAACCTGCTTTGGTT+AGG | - | chr3.4:77829936-77829955 | MS.gene044331:intron | 45.0% | |
| TCAAGATGCTAATGCTGCTG+AGG | - | chr3.4:77830778-77830797 | MS.gene044331:intron | 45.0% | |
| TCAGAACACCTAACCAAAGC+AGG | + | chr3.4:77829947-77829966 | None:intergenic | 45.0% | |
| TCCTTGAATGTCTTCGAGCA+AGG | + | chr3.4:77829895-77829914 | None:intergenic | 45.0% | |
| TGAAAAACGCCTAACAAGGG+TGG | - | chr3.4:77831586-77831605 | MS.gene044331:CDS | 45.0% | |
| TTGTAGCAGGACAGTATACC+TGG | + | chr3.4:77830568-77830587 | None:intergenic | 45.0% | |
| ! | CAATCGAGCCTTTCCTTTTC+TGG | - | chr3.4:77831308-77831327 | MS.gene044331:intron | 45.0% |
| !! | AAGCACTGGTGAGACTGTTT+GGG | - | chr3.4:77830890-77830909 | MS.gene044331:intron | 45.0% |
| !! | AGCTTCTGGTTTGGCTCAAA+GGG | + | chr3.4:77831978-77831997 | None:intergenic | 45.0% |
| !! | TAAGCACTGGTGAGACTGTT+TGG | - | chr3.4:77830889-77830908 | MS.gene044331:intron | 45.0% |
| !! | TAGCTTCTGGTTTGGCTCAA+AGG | + | chr3.4:77831979-77831998 | None:intergenic | 45.0% |
| !! | TTTGCTTGATTCGGGCTTTG+AGG | + | chr3.4:77830272-77830291 | None:intergenic | 45.0% |
| AAGAAAGTTCGGCCGACGAA+GGG | + | chr3.4:77830069-77830088 | None:intergenic | 50.0% | |
| AGTGAGATATGCCTCTCGCA+AGG | - | chr3.4:77831885-77831904 | MS.gene044331:CDS | 50.0% | |
| CATTGGTAGATCCATCTGGG+CGG | + | chr3.4:77830312-77830331 | None:intergenic | 50.0% | |
| CCCTTGCTCGAAGACATTCA+AGG | - | chr3.4:77829891-77829910 | MS.gene044331:CDS | 50.0% | |
| CGCGGTTCTTTGCTTGATTC+GGG | + | chr3.4:77830280-77830299 | None:intergenic | 50.0% | |
| CGGCCGAACTTTCTTCGATA+TGG | - | chr3.4:77830074-77830093 | MS.gene044331:intron | 50.0% | |
| GATATGCCTCTCGCAAGGAA+AGG | - | chr3.4:77831890-77831909 | MS.gene044331:CDS | 50.0% | |
| GCTTCAGGGCAATTTGTAGC+AGG | + | chr3.4:77830581-77830600 | None:intergenic | 50.0% | |
| GTCATAATCATAGGCCTCGC+CGG | + | chr3.4:77831956-77831975 | None:intergenic | 50.0% | |
| TTCTCATGGGAGAACCTCCT+TGG | - | chr3.4:77831387-77831406 | MS.gene044331:intron | 50.0% | |
| TTGTGGAGATCAACGGTCCA+TGG | - | chr3.4:77829802-77829821 | MS.gene044331:CDS | 50.0% | |
| !! | AAGGGCCGGTTTGTTAAAGC+CGG | - | chr3.4:77831934-77831953 | MS.gene044331:CDS | 50.0% |
| !! | TTCGGGCTTTGAGGATGTAC+CGG | + | chr3.4:77830263-77830282 | None:intergenic | 50.0% |
| AGGAAAGGGCTGATGTCCGA+AGG | - | chr3.4:77831905-77831924 | MS.gene044331:CDS | 55.0% | |
| AGTTCGTCCCCACCCTTGTT+AGG | + | chr3.4:77831598-77831617 | None:intergenic | 55.0% | |
| ATAGACACGCTGCATCGGAG+CGG | + | chr3.4:77829835-77829854 | None:intergenic | 55.0% | |
| ATCAGCCCTTTCCTTGCGAG+AGG | + | chr3.4:77831899-77831918 | None:intergenic | 55.0% | |
| GAAGAAAGTTCGGCCGACGA+AGG | + | chr3.4:77830070-77830089 | None:intergenic | 55.0% | |
| ! | CTACTCAGGTTGCCCTTCGT+CGG | - | chr3.4:77830054-77830073 | MS.gene044331:intron | 55.0% |
| !! | CCTCGCCGGCTTTAACAAAC+CGG | + | chr3.4:77831942-77831961 | None:intergenic | 55.0% |
| ATCGGAGCGGCAGTACACCA+TGG | + | chr3.4:77829822-77829841 | None:intergenic | 60.0% | |
| GATGCTGCAGTGCTGCATGC+TGG | + | chr3.4:77831227-77831246 | None:intergenic | 60.0% | |
| GGGGCAAAAGCCATGTCTGC+TGG | - | chr3.4:77830743-77830762 | MS.gene044331:intron | 60.0% | |
| ! | GATGTCCGAAGGCGCGTGAA+GGG | - | chr3.4:77831916-77831935 | MS.gene044331:CDS | 60.0% |
| ! | TGATGTCCGAAGGCGCGTGA+AGG | - | chr3.4:77831915-77831934 | MS.gene044331:CDS | 60.0% |
| !! | CCGGTTTGTTAAAGCCGGCG+AGG | - | chr3.4:77831939-77831958 | MS.gene044331:CDS | 60.0% |
| CGTATGCTCGACCGCCCAGA+TGG | - | chr3.4:77830298-77830317 | MS.gene044331:CDS | 65.0% | |
| TCTGGGCGGTCGAGCATACG+CGG | + | chr3.4:77830298-77830317 | None:intergenic | 65.0% | |
| ACCGGCCCTTCACGCGCCTT+CGG | + | chr3.4:77831924-77831943 | None:intergenic | 70.0% | |
| ! | TCCGAAGGCGCGTGAAGGGC+CGG | - | chr3.4:77831920-77831939 | MS.gene044331:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 77829782 | 77832002 | 77829782 | ID=MS.gene044331 |
| chr3.4 | mRNA | 77829782 | 77832002 | 77829782 | ID=MS.gene044331.t1;Parent=MS.gene044331 |
| chr3.4 | exon | 77831439 | 77832002 | 77831439 | ID=MS.gene044331.t1.exon1;Parent=MS.gene044331.t1 |
| chr3.4 | CDS | 77831439 | 77832002 | 77831439 | ID=cds.MS.gene044331.t1;Parent=MS.gene044331.t1 |
| chr3.4 | exon | 77830985 | 77831215 | 77830985 | ID=MS.gene044331.t1.exon2;Parent=MS.gene044331.t1 |
| chr3.4 | CDS | 77830985 | 77831215 | 77830985 | ID=cds.MS.gene044331.t1;Parent=MS.gene044331.t1 |
| chr3.4 | exon | 77830283 | 77830593 | 77830283 | ID=MS.gene044331.t1.exon3;Parent=MS.gene044331.t1 |
| chr3.4 | CDS | 77830283 | 77830593 | 77830283 | ID=cds.MS.gene044331.t1;Parent=MS.gene044331.t1 |
| chr3.4 | exon | 77829782 | 77829911 | 77829782 | ID=MS.gene044331.t1.exon4;Parent=MS.gene044331.t1 |
| chr3.4 | CDS | 77829782 | 77829911 | 77829782 | ID=cds.MS.gene044331.t1;Parent=MS.gene044331.t1 |
| Gene Sequence |
| Protein sequence |
