Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25698.t1 | XP_003601522.1 | 99 | 411 | 4 | 0 | 1 | 411 | 1 | 411 | 3.20E-198 | 701 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25698.t1 | Q9SSE5 | 55.1 | 423 | 127 | 9 | 1 | 411 | 1 | 372 | 3.9e-80 | 300.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25698.t1 | G7J8U1 | 99.0 | 411 | 4 | 0 | 1 | 411 | 1 | 411 | 2.3e-198 | 701.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene25698.t1 | TF | C2C2-CO-like |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050702 | MS.gene25698 | -0.911462 | 5.92E-83 | -1.69E-46 |
| MS.gene050743 | MS.gene25698 | 0.805575 | 1.25E-49 | -1.69E-46 |
| MS.gene058551 | MS.gene25698 | 0.911934 | 3.46E-83 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene88720 | MS.gene25698 | PPI |
| MS.gene25698 | MS.gene044331 | PPI |
| MS.gene70471 | MS.gene25698 | PPI |
| MS.gene43742 | MS.gene25698 | PPI |
| MS.gene25698 | MS.gene009935 | PPI |
| MS.gene44846 | MS.gene25698 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25698.t1 | MTR_3g082630 | 99.027 | 411 | 4 | 0 | 1 | 411 | 1 | 411 | 0.0 | 845 |
| MS.gene25698.t1 | MTR_5g069480 | 68.675 | 415 | 119 | 7 | 1 | 410 | 1 | 409 | 0.0 | 513 |
| MS.gene25698.t1 | MTR_5g069480 | 65.556 | 270 | 86 | 5 | 1 | 265 | 1 | 268 | 3.63e-117 | 343 |
| MS.gene25698.t1 | MTR_2g088900 | 33.630 | 449 | 226 | 13 | 1 | 405 | 97 | 517 | 6.74e-62 | 208 |
| MS.gene25698.t1 | MTR_7g108150 | 50.000 | 100 | 50 | 0 | 5 | 104 | 20 | 119 | 2.86e-29 | 117 |
| MS.gene25698.t1 | MTR_7g108150 | 62.500 | 48 | 18 | 0 | 348 | 395 | 322 | 369 | 9.04e-12 | 66.6 |
| MS.gene25698.t1 | MTR_1g109350 | 44.860 | 107 | 57 | 1 | 1 | 107 | 1 | 105 | 1.38e-24 | 103 |
| MS.gene25698.t1 | MTR_7g083540 | 35.971 | 139 | 81 | 2 | 1 | 132 | 1 | 138 | 1.38e-21 | 95.9 |
| MS.gene25698.t1 | MTR_7g083540 | 56.061 | 66 | 29 | 0 | 331 | 396 | 318 | 383 | 1.66e-14 | 75.1 |
| MS.gene25698.t1 | MTR_1g013450 | 44.048 | 84 | 46 | 1 | 4 | 86 | 5 | 88 | 6.57e-17 | 81.3 |
| MS.gene25698.t1 | MTR_3g105710 | 39.216 | 102 | 61 | 1 | 4 | 104 | 5 | 106 | 4.94e-16 | 78.6 |
| MS.gene25698.t1 | MTR_3g105710 | 45.833 | 72 | 36 | 1 | 333 | 401 | 208 | 279 | 4.15e-11 | 63.9 |
| MS.gene25698.t1 | MTR_4g128930 | 38.889 | 90 | 54 | 1 | 4 | 92 | 21 | 110 | 4.79e-15 | 76.6 |
| MS.gene25698.t1 | MTR_7g018170 | 41.758 | 91 | 52 | 1 | 5 | 94 | 26 | 116 | 2.05e-14 | 74.7 |
| MS.gene25698.t1 | MTR_4g067320 | 38.542 | 96 | 49 | 2 | 1 | 86 | 1 | 96 | 1.32e-13 | 70.5 |
| MS.gene25698.t1 | MTR_4g067320 | 38.542 | 96 | 49 | 2 | 1 | 86 | 1 | 96 | 1.38e-13 | 70.5 |
| MS.gene25698.t1 | MTR_5g021580 | 38.095 | 84 | 44 | 1 | 1 | 76 | 1 | 84 | 2.82e-11 | 62.8 |
| MS.gene25698.t1 | MTR_5g021580 | 38.095 | 84 | 44 | 1 | 1 | 76 | 1 | 84 | 3.42e-11 | 62.4 |
| MS.gene25698.t1 | MTR_1g023260 | 34.021 | 97 | 53 | 2 | 1 | 86 | 1 | 97 | 3.78e-11 | 62.4 |
| MS.gene25698.t1 | MTR_1g023260 | 34.021 | 97 | 53 | 2 | 1 | 86 | 1 | 97 | 3.96e-11 | 63.5 |
| MS.gene25698.t1 | MTR_4g071200 | 35.052 | 97 | 52 | 2 | 1 | 86 | 1 | 97 | 5.43e-11 | 62.4 |
| MS.gene25698.t1 | MTR_3g113070 | 33.673 | 98 | 53 | 2 | 1 | 86 | 1 | 98 | 8.79e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25698.t1 | AT3G07650 | 52.719 | 423 | 137 | 10 | 1 | 411 | 1 | 372 | 1.31e-142 | 411 |
| MS.gene25698.t1 | AT3G07650 | 52.719 | 423 | 137 | 10 | 1 | 411 | 1 | 372 | 1.31e-142 | 411 |
| MS.gene25698.t1 | AT3G07650 | 52.719 | 423 | 137 | 10 | 1 | 411 | 1 | 372 | 1.31e-142 | 411 |
| MS.gene25698.t1 | AT3G07650 | 52.719 | 423 | 137 | 10 | 1 | 411 | 1 | 372 | 1.31e-142 | 411 |
| MS.gene25698.t1 | AT5G48250 | 53.810 | 420 | 138 | 11 | 1 | 411 | 1 | 373 | 8.29e-141 | 406 |
| MS.gene25698.t1 | AT3G21880 | 34.063 | 411 | 210 | 11 | 5 | 408 | 9 | 365 | 2.29e-61 | 203 |
| MS.gene25698.t1 | AT3G21880 | 34.063 | 411 | 210 | 11 | 5 | 408 | 9 | 365 | 2.29e-61 | 203 |
| MS.gene25698.t1 | AT3G21880 | 34.063 | 411 | 210 | 11 | 5 | 408 | 5 | 361 | 2.69e-61 | 202 |
| MS.gene25698.t1 | AT4G15250 | 53.153 | 111 | 50 | 2 | 5 | 115 | 5 | 113 | 6.47e-34 | 129 |
| MS.gene25698.t1 | AT4G15250 | 51.020 | 98 | 45 | 1 | 307 | 404 | 232 | 326 | 7.61e-20 | 90.1 |
| MS.gene25698.t1 | AT2G33500 | 47.170 | 106 | 55 | 1 | 5 | 109 | 12 | 117 | 2.93e-30 | 120 |
| MS.gene25698.t1 | AT2G33500 | 60.784 | 51 | 20 | 0 | 346 | 396 | 348 | 398 | 1.50e-12 | 69.3 |
| MS.gene25698.t1 | AT2G33500 | 47.170 | 106 | 55 | 1 | 5 | 109 | 12 | 117 | 2.98e-30 | 120 |
| MS.gene25698.t1 | AT2G33500 | 60.784 | 51 | 20 | 0 | 346 | 396 | 349 | 399 | 1.50e-12 | 69.3 |
| MS.gene25698.t1 | AT1G68190 | 31.395 | 258 | 141 | 6 | 1 | 227 | 10 | 262 | 3.31e-29 | 117 |
| MS.gene25698.t1 | AT1G68190 | 31.395 | 258 | 141 | 6 | 1 | 227 | 10 | 262 | 3.31e-29 | 117 |
| MS.gene25698.t1 | AT1G68190 | 31.395 | 258 | 141 | 6 | 1 | 227 | 39 | 291 | 3.72e-29 | 117 |
| MS.gene25698.t1 | AT1G28050 | 39.041 | 146 | 86 | 2 | 5 | 147 | 9 | 154 | 6.35e-29 | 117 |
| MS.gene25698.t1 | AT1G28050 | 61.111 | 54 | 21 | 0 | 346 | 399 | 377 | 430 | 4.50e-13 | 70.9 |
| MS.gene25698.t1 | AT2G47890 | 36.478 | 159 | 93 | 3 | 4 | 162 | 12 | 162 | 2.42e-25 | 103 |
| MS.gene25698.t1 | AT2G47890 | 36.478 | 159 | 93 | 3 | 4 | 162 | 12 | 162 | 7.36e-25 | 103 |
| MS.gene25698.t1 | AT2G47890 | 36.478 | 159 | 93 | 3 | 4 | 162 | 12 | 162 | 9.44e-25 | 104 |
| MS.gene25698.t1 | AT5G24930 | 43.333 | 90 | 50 | 1 | 4 | 92 | 5 | 94 | 4.11e-17 | 82.8 |
| MS.gene25698.t1 | AT5G24930 | 55.769 | 52 | 23 | 0 | 344 | 395 | 285 | 336 | 3.11e-11 | 65.1 |
| MS.gene25698.t1 | AT2G24790 | 42.222 | 90 | 51 | 1 | 4 | 92 | 7 | 96 | 8.81e-16 | 76.6 |
| MS.gene25698.t1 | AT2G24790 | 40.187 | 107 | 62 | 2 | 4 | 108 | 7 | 113 | 9.40e-16 | 77.8 |
| MS.gene25698.t1 | AT2G24790 | 50.877 | 57 | 28 | 0 | 347 | 403 | 222 | 278 | 4.75e-11 | 63.9 |
| MS.gene25698.t1 | AT5G15840 | 43.956 | 91 | 50 | 1 | 5 | 94 | 20 | 110 | 9.78e-15 | 75.5 |
| MS.gene25698.t1 | AT5G15840 | 43.956 | 91 | 50 | 1 | 5 | 94 | 20 | 110 | 1.59e-14 | 73.9 |
| MS.gene25698.t1 | AT5G15850 | 44.186 | 86 | 47 | 1 | 5 | 89 | 12 | 97 | 2.34e-14 | 74.3 |
| MS.gene25698.t1 | AT3G02380 | 40.000 | 100 | 53 | 2 | 5 | 97 | 16 | 115 | 1.35e-13 | 72.0 |
| MS.gene25698.t1 | AT3G02380 | 62.500 | 48 | 18 | 0 | 348 | 395 | 272 | 319 | 5.23e-12 | 67.0 |
| MS.gene25698.t1 | AT5G57660 | 30.216 | 139 | 92 | 2 | 5 | 142 | 22 | 156 | 2.57e-12 | 68.2 |
| MS.gene25698.t1 | AT2G31380 | 36.458 | 96 | 51 | 2 | 1 | 86 | 1 | 96 | 2.65e-12 | 66.6 |
| MS.gene25698.t1 | AT1G06040 | 37.500 | 96 | 50 | 2 | 1 | 86 | 1 | 96 | 3.68e-12 | 65.1 |
| MS.gene25698.t1 | AT1G06040 | 37.500 | 96 | 50 | 2 | 1 | 86 | 1 | 96 | 4.37e-12 | 66.2 |
| MS.gene25698.t1 | AT4G38960 | 36.905 | 84 | 45 | 1 | 1 | 76 | 1 | 84 | 6.94e-11 | 61.6 |
| MS.gene25698.t1 | AT4G38960 | 36.905 | 84 | 45 | 1 | 1 | 76 | 1 | 84 | 6.94e-11 | 61.6 |
| MS.gene25698.t1 | AT2G21320 | 39.286 | 84 | 43 | 1 | 1 | 76 | 1 | 84 | 6.94e-11 | 61.2 |
| MS.gene25698.t1 | AT1G75540 | 34.343 | 99 | 52 | 2 | 1 | 86 | 1 | 99 | 8.57e-11 | 63.2 |
Find 90 sgRNAs with CRISPR-Local
Find 143 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTGGATTTAACAGTTTATT+TGG | 0.095234 | 3.3:-69375035 | MS.gene25698:CDS |
| GTGGATTTAACAGTTTATTT+GGG | 0.284402 | 3.3:-69375034 | MS.gene25698:CDS |
| CGCGGTTCTTTGCTTGATTC+GGG | 0.309949 | 3.3:+69375486 | None:intergenic |
| AGCTACTGATCTACCTGATT+TGG | 0.310422 | 3.3:-69375538 | MS.gene25698:CDS |
| ACGCGGTTCTTTGCTTGATT+CGG | 0.339112 | 3.3:+69375485 | None:intergenic |
| GGATGAAATGGATATAAATA+TGG | 0.347846 | 3.3:-69375117 | MS.gene25698:CDS |
| CGATTTCGATATGGATGAAA+TGG | 0.355447 | 3.3:-69375129 | MS.gene25698:CDS |
| TACCTTTGGTACACATTCAT+TGG | 0.361210 | 3.3:+69375437 | None:intergenic |
| GATGCTGCAGTGCTGCATGC+TGG | 0.368884 | 3.3:+69374525 | None:intergenic |
| TATGTTCAGGGTTCATCAAT+TGG | 0.370264 | 3.3:-69374567 | MS.gene25698:intron |
| ATTATACACTCAGTAGCTTC+TGG | 0.377897 | 3.3:+69373760 | None:intergenic |
| CAGCCGAACTTTCTTCGATA+TGG | 0.382675 | 3.3:-69375689 | MS.gene25698:CDS |
| ACCGGCCCTTCACGCGCCTT+CGG | 0.385614 | 3.3:+69373828 | None:intergenic |
| TCATCATCATCGCTTGCGTC+AGG | 0.392076 | 3.3:+69375164 | None:intergenic |
| GATCGTTTGCCTCTTATGAT+TGG | 0.395417 | 3.3:+69375737 | None:intergenic |
| CACTTTGTCAGAATTGTGAT+TGG | 0.398736 | 3.3:-69375785 | MS.gene25698:CDS |
| ATGCAGTTCACAACCTGCTT+TGG | 0.404101 | 3.3:-69375832 | MS.gene25698:CDS |
| CCTCAGCAGCATTAGCATCT+TGG | 0.411307 | 3.3:+69374979 | None:intergenic |
| GTTCACAACCTGCTTTGGTT+AGG | 0.412214 | 3.3:-69375827 | MS.gene25698:CDS |
| TAGCTTCTGGTTTGGCTCAA+AGG | 0.415587 | 3.3:+69373773 | None:intergenic |
| ACACTCAGTAGCTTCTGGTT+TGG | 0.416653 | 3.3:+69373765 | None:intergenic |
| CATTCATTGGTAGATCCATC+TGG | 0.421154 | 3.3:+69375450 | None:intergenic |
| AGCTTCTTCAATGCTTCTCA+TGG | 0.422785 | 3.3:-69374376 | MS.gene25698:CDS |
| AGCTTCTGGTTTGGCTCAAA+GGG | 0.425533 | 3.3:+69373774 | None:intergenic |
| CTACATCTTCCAATCATAAG+AGG | 0.437225 | 3.3:-69375746 | MS.gene25698:CDS |
| CAGAAAAGGAAAGGCTCGAT+TGG | 0.437549 | 3.3:+69374442 | None:intergenic |
| GCTCAAAGGGTCATAATCAT+AGG | 0.437780 | 3.3:+69373787 | None:intergenic |
| AGCATTAGCATCTTGGCAAT+TGG | 0.442849 | 3.3:+69374986 | None:intergenic |
| TGTCAGAATTGTGATTGGTT+AGG | 0.444655 | 3.3:-69375780 | MS.gene25698:CDS |
| GCGAAAGACTTATCCAAATC+AGG | 0.451210 | 3.3:+69375525 | None:intergenic |
| TGTAGTTGTAGCTCATTCTC+AGG | 0.468722 | 3.3:+69374327 | None:intergenic |
| AGACCATATCGAAGAAAGTT+CGG | 0.481106 | 3.3:+69375686 | None:intergenic |
| TCCGAAGGCGCGTGAAGGGC+CGG | 0.483385 | 3.3:-69373829 | MS.gene25698:CDS |
| TTTGCAGTAATACAAAGATT+CGG | 0.484517 | 3.3:+69374471 | None:intergenic |
| GGGGCAAAAGCCATGTCTGC+TGG | 0.484909 | 3.3:-69375014 | MS.gene25698:CDS |
| TTCTCATGGGAGAACCTCCT+TGG | 0.487490 | 3.3:-69374362 | MS.gene25698:CDS |
| TCATGAGTATTAATGAGAAT+AGG | 0.491713 | 3.3:-69375608 | MS.gene25698:CDS |
| TCTGTATGGCGATTTCGATA+TGG | 0.492638 | 3.3:-69375138 | MS.gene25698:CDS |
| AGAAAAGGAAAGGCTCGATT+GGG | 0.501130 | 3.3:+69374443 | None:intergenic |
| ATTAATGAAGACGGTGGTGC+TGG | 0.505210 | 3.3:-69374417 | MS.gene25698:CDS |
| GCGTCAGGGCAATTTGTAGC+AGG | 0.511320 | 3.3:+69375179 | None:intergenic |
| GTCTTCATTAATACCAGAAA+AGG | 0.513355 | 3.3:+69374428 | None:intergenic |
| ATATGCCTCTCGCAAGGAAA+GGG | 0.514733 | 3.3:-69373858 | MS.gene25698:CDS |
| TAATGAGAATAGGAGTGCTT+GGG | 0.516509 | 3.3:-69375598 | MS.gene25698:CDS |
| CCCTTGCTCGAAGACATTCA+AGG | 0.516832 | 3.3:-69375872 | MS.gene25698:CDS |
| TGATGTCCGAAGGCGCGTGA+AGG | 0.519956 | 3.3:-69373834 | MS.gene25698:CDS |
| GTCATAATCATAGGCCTCGC+CGG | 0.520189 | 3.3:+69373796 | None:intergenic |
| TTAATGAGAATAGGAGTGCT+TGG | 0.522192 | 3.3:-69375599 | MS.gene25698:CDS |
| CAAACGATCAATTGCTACTC+AGG | 0.524349 | 3.3:-69375723 | MS.gene25698:CDS |
| CCTCGCCGGCTTTAACAAAC+CGG | 0.526175 | 3.3:+69373810 | None:intergenic |
| ACTACATGTGAGCAAGAACT+AGG | 0.526382 | 3.3:-69375633 | MS.gene25698:CDS |
| AAGAAAGTTCGGCTGACGAA+GGG | 0.532257 | 3.3:+69375697 | None:intergenic |
| TCCTTGAATGTCTTCGAGCA+AGG | 0.533062 | 3.3:+69375871 | None:intergenic |
| GATGTCCGAAGGCGCGTGAA+GGG | 0.533693 | 3.3:-69373833 | MS.gene25698:CDS |
| AAGGGCCGGTTTGTTAAAGC+CGG | 0.538044 | 3.3:-69373815 | MS.gene25698:CDS |
| CGTATGCTCGACCGCCCAGA+TGG | 0.548257 | 3.3:-69375465 | MS.gene25698:CDS |
| AATTGTGATTGGTTAGGTCA+TGG | 0.558365 | 3.3:-69375774 | MS.gene25698:CDS |
| GAAGAAAGTTCGGCTGACGA+AGG | 0.562664 | 3.3:+69375696 | None:intergenic |
| ATTGGAATCTCCAGCAGACA+TGG | 0.566425 | 3.3:+69375004 | None:intergenic |
| GCTTCTTCAATGCTTCTCAT+GGG | 0.566506 | 3.3:-69374375 | MS.gene25698:CDS |
| TCAGAACACCTAACCAAAGC+AGG | 0.567607 | 3.3:+69375819 | None:intergenic |
| CATGTAGTTTCACTCAGAGA+AGG | 0.576596 | 3.3:+69375648 | None:intergenic |
| CAAACATAGACACGCTGCAT+CGG | 0.578296 | 3.3:+69375926 | None:intergenic |
| TAGTGCTGTTATGCGATACA+AGG | 0.579883 | 3.3:-69374289 | MS.gene25698:CDS |
| TACCAATGAATGTGTACCAA+AGG | 0.580738 | 3.3:-69375439 | MS.gene25698:intron |
| CCTTGAATGTCTTCGAGCAA+GGG | 0.583830 | 3.3:+69375872 | None:intergenic |
| GATATGCCTCTCGCAAGGAA+AGG | 0.583865 | 3.3:-69373859 | MS.gene25698:CDS |
| TTCGGGCATTGAGAATGTAC+CGG | 0.588538 | 3.3:+69375503 | None:intergenic |
| TGGATTTAACAGTTTATTTG+GGG | 0.588596 | 3.3:-69375033 | MS.gene25698:CDS |
| TTGTGGAGATCAGAGGTCGA+TGG | 0.588789 | 3.3:-69375961 | MS.gene25698:CDS |
| GATTTGGATAAGTCTTTCGC+CGG | 0.589605 | 3.3:-69375522 | MS.gene25698:CDS |
| CATTAATACCAGAAAAGGAA+AGG | 0.592524 | 3.3:+69374433 | None:intergenic |
| AGGAAAGGGCTGATGTCCGA+AGG | 0.595717 | 3.3:-69373844 | MS.gene25698:CDS |
| ATGTAGTTTCACTCAGAGAA+GGG | 0.599442 | 3.3:+69375649 | None:intergenic |
| CCAAGATGCTAATGCTGCTG+AGG | 0.599606 | 3.3:-69374979 | MS.gene25698:intron |
| CATCATCATCGCTTGCGTCA+GGG | 0.601417 | 3.3:+69375165 | None:intergenic |
| ATCAGCCCTTTCCTTGCGAG+AGG | 0.604664 | 3.3:+69373853 | None:intergenic |
| ATTCATTGGTAGATCCATCT+GGG | 0.610627 | 3.3:+69375451 | None:intergenic |
| AAAGCTCTTCAGAATGAGTG+AGG | 0.617795 | 3.3:+69375066 | None:intergenic |
| GCTGGAGACTATCAAGATTG+TGG | 0.619737 | 3.3:-69374399 | MS.gene25698:CDS |
| AGTGAGATATGCCTCTCGCA+AGG | 0.625277 | 3.3:-69373864 | MS.gene25698:CDS |
| TCTGGTATTAATGAAGACGG+TGG | 0.629157 | 3.3:-69374423 | MS.gene25698:CDS |
| CCGGTTTGTTAAAGCCGGCG+AGG | 0.638136 | 3.3:-69373810 | MS.gene25698:CDS |
| TCTGGGCGGTCGAGCATACG+CGG | 0.641340 | 3.3:+69375468 | None:intergenic |
| TCAGGACAAGTGTTTAACCA+AGG | 0.666255 | 3.3:+69374345 | None:intergenic |
| AAGCTCTTCAGAATGAGTGA+GGG | 0.667176 | 3.3:+69375067 | None:intergenic |
| GGACAAGTGTTTAACCAAGG+AGG | 0.698616 | 3.3:+69374348 | None:intergenic |
| ATCGCATAACAGCACTACAG+CGG | 0.703445 | 3.3:+69374295 | None:intergenic |
| ATAGACACGCTGCATCGGAG+CGG | 0.713055 | 3.3:+69375931 | None:intergenic |
| CATTGGTAGATCCATCTGGG+CGG | 0.761162 | 3.3:+69375454 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTGATTGTTATTATCTTTT+AGG | - | chr3.3:69375800-69375819 | MS.gene25698:CDS | 15.0% |
| !! | TCTAATTGGATAAAAATGTA+AGG | + | chr3.3:69374515-69374534 | None:intergenic | 20.0% |
| !!! | AATAAGTTGAAAACAACTTA+TGG | + | chr3.3:69374938-69374957 | None:intergenic | 20.0% |
| !!! | ACATCTAATTTTTATGTTCA+GGG | - | chr3.3:69375171-69375190 | MS.gene25698:CDS | 20.0% |
| !!! | TACATCTAATTTTTATGTTC+AGG | - | chr3.3:69375170-69375189 | MS.gene25698:CDS | 20.0% |
| ! | AACATTCAGATATATACCTT+TGG | + | chr3.3:69374330-69374349 | None:intergenic | 25.0% |
| ! | ACTTTCCCTTAAAAGATATA+AGG | - | chr3.3:69375616-69375635 | MS.gene25698:CDS | 25.0% |
| ! | ATACAAATTGTTTACACCTT+CGG | - | chr3.3:69375120-69375139 | MS.gene25698:CDS | 25.0% |
| ! | GGATGAAATGGATATAAATA+TGG | - | chr3.3:69374633-69374652 | MS.gene25698:intron | 25.0% |
| ! | GGTAACATGATAATCAAATT+AGG | + | chr3.3:69375700-69375719 | None:intergenic | 25.0% |
| ! | GTGGATTTAACAGTTTATTT+GGG | - | chr3.3:69374716-69374735 | MS.gene25698:intron | 25.0% |
| ! | TCATGAGTATTAATGAGAAT+AGG | - | chr3.3:69374142-69374161 | MS.gene25698:intron | 25.0% |
| ! | TGGATTTAACAGTTTATTTG+GGG | - | chr3.3:69374717-69374736 | MS.gene25698:intron | 25.0% |
| ! | TTATATATTAGCATAAGCAC+TGG | - | chr3.3:69374869-69374888 | MS.gene25698:intron | 25.0% |
| ! | TTTGCAGTAATACAAAGATT+CGG | + | chr3.3:69375282-69375301 | None:intergenic | 25.0% |
| !! | TCAGACCTTATATCTTTTAA+GGG | + | chr3.3:69375624-69375643 | None:intergenic | 25.0% |
| !! | TTCAGACCTTATATCTTTTA+AGG | + | chr3.3:69375625-69375644 | None:intergenic | 25.0% |
| !!! | TTTTCTGGTATTAATGAAGA+CGG | - | chr3.3:69375324-69375343 | MS.gene25698:intron | 25.0% |
| ACGCAATCAAATGATAGAAA+AGG | + | chr3.3:69374835-69374854 | None:intergenic | 30.0% | |
| AGAGGAAAAACACATTCAAT+AGG | + | chr3.3:69375559-69375578 | None:intergenic | 30.0% | |
| ATGAGAGTTAGCTATCTAAT+TGG | + | chr3.3:69374529-69374548 | None:intergenic | 30.0% | |
| ATGATTAAGCTATTGCTCTA+TGG | + | chr3.3:69374410-69374429 | None:intergenic | 30.0% | |
| CATTAATACCAGAAAAGGAA+AGG | + | chr3.3:69375320-69375339 | None:intergenic | 30.0% | |
| GGATTTCAATTATAAACCTC+AGG | + | chr3.3:69374494-69374513 | None:intergenic | 30.0% | |
| GTCTTCATTAATACCAGAAA+AGG | + | chr3.3:69375325-69375344 | None:intergenic | 30.0% | |
| TAGAGCAATAGCTTAATCAT+TGG | - | chr3.3:69374410-69374429 | MS.gene25698:CDS | 30.0% | |
| TGAAAAGAGTTTCTAAACAG+TGG | - | chr3.3:69375641-69375660 | MS.gene25698:CDS | 30.0% | |
| TGCTAACAAGTTAGGAATTA+CGG | - | chr3.3:69375725-69375744 | MS.gene25698:CDS | 30.0% | |
| TTTCTATCATTTGATTGCGT+TGG | - | chr3.3:69374835-69374854 | MS.gene25698:intron | 30.0% | |
| ! | CTGTTTTCATAAGCTTATCT+TGG | + | chr3.3:69374973-69374992 | None:intergenic | 30.0% |
| ! | GAGAACTATGATGAACTTTT+TGG | - | chr3.3:69374655-69374674 | MS.gene25698:intron | 30.0% |
| ! | GCGTTTTTCATTCAAAAAAG+AGG | + | chr3.3:69375577-69375596 | None:intergenic | 30.0% |
| ! | GGTGGATTTAACAGTTTATT+TGG | - | chr3.3:69374715-69374734 | MS.gene25698:intron | 30.0% |
| ! | GGTTACATATGTGATTTTTG+TGG | - | chr3.3:69373772-69373791 | MS.gene25698:CDS | 30.0% |
| !! | CTATGATGAACTTTTTGGTA+TGG | - | chr3.3:69374660-69374679 | MS.gene25698:intron | 30.0% |
| !! | TCTGAAGAGCTTTTTGAAAA+TGG | - | chr3.3:69374694-69374713 | MS.gene25698:intron | 30.0% |
| !! | TTCAGTTTTAGTGCTCAATA+TGG | + | chr3.3:69375259-69375278 | None:intergenic | 30.0% |
| ACAGAGCTTATGTCTACTAT+TGG | - | chr3.3:69375082-69375101 | MS.gene25698:CDS | 35.0% | |
| AGACCATATCGAAGAAAGTT+CGG | + | chr3.3:69374067-69374086 | None:intergenic | 35.0% | |
| AGCTTATCTTGGAGAACTTA+TGG | + | chr3.3:69374962-69374981 | None:intergenic | 35.0% | |
| ATGTAGTTTCACTCAGAGAA+GGG | + | chr3.3:69374104-69374123 | None:intergenic | 35.0% | |
| ATTCATTGGTAGATCCATCT+GGG | + | chr3.3:69374302-69374321 | None:intergenic | 35.0% | |
| CACTTTGTCAGAATTGTGAT+TGG | - | chr3.3:69373965-69373984 | MS.gene25698:intron | 35.0% | |
| CGATTTCGATATGGATGAAA+TGG | - | chr3.3:69374621-69374640 | MS.gene25698:intron | 35.0% | |
| CTACATCTTCCAATCATAAG+AGG | - | chr3.3:69374004-69374023 | MS.gene25698:intron | 35.0% | |
| CTCTACTTAAAATCAACCGA+AGG | + | chr3.3:69375139-69375158 | None:intergenic | 35.0% | |
| GAATGAAAAACGCCTAACAA+GGG | - | chr3.3:69375584-69375603 | MS.gene25698:CDS | 35.0% | |
| GCATTACTCACTTTACAAAC+TGG | - | chr3.3:69375663-69375682 | MS.gene25698:CDS | 35.0% | |
| GCTAACAAGTTAGGAATTAC+GGG | - | chr3.3:69375726-69375745 | MS.gene25698:CDS | 35.0% | |
| TACCAATGAATGTGTACCAA+AGG | - | chr3.3:69374311-69374330 | MS.gene25698:CDS | 35.0% | |
| TACCTTTGGTACACATTCAT+TGG | + | chr3.3:69374316-69374335 | None:intergenic | 35.0% | |
| TAGTAGACATAAGCTCTGTT+TGG | + | chr3.3:69375081-69375100 | None:intergenic | 35.0% | |
| TATGTTCAGGGTTCATCAAT+TGG | - | chr3.3:69375183-69375202 | MS.gene25698:CDS | 35.0% | |
| TGAATGAAAAACGCCTAACA+AGG | - | chr3.3:69375583-69375602 | MS.gene25698:CDS | 35.0% | |
| ! | AATTGTGATTGGTTAGGTCA+TGG | - | chr3.3:69373976-69373995 | MS.gene25698:intron | 35.0% |
| ! | GATGATGATGATGATCTGTA+TGG | - | chr3.3:69374598-69374617 | MS.gene25698:intron | 35.0% |
| ! | TCAGACACGTTCTGATTTTT+CGG | + | chr3.3:69374183-69374202 | None:intergenic | 35.0% |
| ! | TGTCAGAATTGTGATTGGTT+AGG | - | chr3.3:69373970-69373989 | MS.gene25698:intron | 35.0% |
| !! | AACTCTCATTGTTGATTTCC+AGG | - | chr3.3:69374540-69374559 | MS.gene25698:CDS | 35.0% |
| !! | GAAGAGCTTTTTGAAAATGG+TGG | - | chr3.3:69374697-69374716 | MS.gene25698:intron | 35.0% |
| !! | TAATGAGAATAGGAGTGCTT+GGG | - | chr3.3:69374152-69374171 | MS.gene25698:intron | 35.0% |
| !! | TCCTAACTTGTTAGCAAAGA+CGG | + | chr3.3:69375721-69375740 | None:intergenic | 35.0% |
| !! | TTAATGAGAATAGGAGTGCT+TGG | - | chr3.3:69374151-69374170 | MS.gene25698:intron | 35.0% |
| AAAGCTCTTCAGAATGAGTG+AGG | + | chr3.3:69374687-69374706 | None:intergenic | 40.0% | |
| AAGCTCTTCAGAATGAGTGA+GGG | + | chr3.3:69374686-69374705 | None:intergenic | 40.0% | |
| ACCGTCTTTGCTAACAAGTT+AGG | - | chr3.3:69375717-69375736 | MS.gene25698:CDS | 40.0% | |
| ACTACATGTGAGCAAGAACT+AGG | - | chr3.3:69374117-69374136 | MS.gene25698:intron | 40.0% | |
| AGAAAAGGAAAGGCTCGATT+GGG | + | chr3.3:69375310-69375329 | None:intergenic | 40.0% | |
| AGCATTAGCATCTTGGCAAT+TGG | + | chr3.3:69374767-69374786 | None:intergenic | 40.0% | |
| AGCTACTGATCTACCTGATT+TGG | - | chr3.3:69374212-69374231 | MS.gene25698:intron | 40.0% | |
| AGCTTCTTCAATGCTTCTCA+TGG | - | chr3.3:69375374-69375393 | MS.gene25698:intron | 40.0% | |
| CAAACGATCAATTGCTACTC+AGG | - | chr3.3:69374027-69374046 | MS.gene25698:intron | 40.0% | |
| CATGTAGTTTCACTCAGAGA+AGG | + | chr3.3:69374105-69374124 | None:intergenic | 40.0% | |
| CATTCATTGGTAGATCCATC+TGG | + | chr3.3:69374303-69374322 | None:intergenic | 40.0% | |
| GATCGTTTGCCTCTTATGAT+TGG | + | chr3.3:69374016-69374035 | None:intergenic | 40.0% | |
| GATTTGGATAAGTCTTTCGC+CGG | - | chr3.3:69374228-69374247 | MS.gene25698:intron | 40.0% | |
| GCGAAAGACTTATCCAAATC+AGG | + | chr3.3:69374228-69374247 | None:intergenic | 40.0% | |
| GCTCAAAGGGTCATAATCAT+AGG | + | chr3.3:69375966-69375985 | None:intergenic | 40.0% | |
| GCTTCTTCAATGCTTCTCAT+GGG | - | chr3.3:69375375-69375394 | MS.gene25698:intron | 40.0% | |
| TAAACGAGTTTCACTACCTG+AGG | - | chr3.3:69374475-69374494 | MS.gene25698:CDS | 40.0% | |
| TCAGGACAAGTGTTTAACCA+AGG | + | chr3.3:69375408-69375427 | None:intergenic | 40.0% | |
| TCTGGTATTAATGAAGACGG+TGG | - | chr3.3:69375327-69375346 | MS.gene25698:intron | 40.0% | |
| TCTGTATGGCGATTTCGATA+TGG | - | chr3.3:69374612-69374631 | MS.gene25698:intron | 40.0% | |
| TGTAGTTGTAGCTCATTCTC+AGG | + | chr3.3:69375426-69375445 | None:intergenic | 40.0% | |
| ! | GTGATTTTTGTGGAGATCAG+AGG | - | chr3.3:69373782-69373801 | MS.gene25698:CDS | 40.0% |
| ! | TAGTGCTGTTATGCGATACA+AGG | - | chr3.3:69375461-69375480 | MS.gene25698:CDS | 40.0% |
| AAAAACGCCTAACAAGGGTG+GGG | - | chr3.3:69375589-69375608 | MS.gene25698:CDS | 45.0% | |
| AAGAAAGTTCGGCTGACGAA+GGG | + | chr3.3:69374056-69374075 | None:intergenic | 45.0% | |
| ACGCGGTTCTTTGCTTGATT+CGG | + | chr3.3:69374268-69374287 | None:intergenic | 45.0% | |
| AGACTGTTTGGGAGAGCTTA+TGG | - | chr3.3:69374894-69374913 | MS.gene25698:intron | 45.0% | |
| AGCTTAATCATTGGCGAAGC+AGG | - | chr3.3:69374419-69374438 | MS.gene25698:CDS | 45.0% | |
| ATATGCCTCTCGCAAGGAAA+GGG | - | chr3.3:69375892-69375911 | MS.gene25698:CDS | 45.0% | |
| ATCGCATAACAGCACTACAG+CGG | + | chr3.3:69375458-69375477 | None:intergenic | 45.0% | |
| ATGCAGTTCACAACCTGCTT+TGG | - | chr3.3:69373918-69373937 | MS.gene25698:intron | 45.0% | |
| ATTAATGAAGACGGTGGTGC+TGG | - | chr3.3:69375333-69375352 | MS.gene25698:intron | 45.0% | |
| ATTGGAATCTCCAGCAGACA+TGG | + | chr3.3:69374749-69374768 | None:intergenic | 45.0% | |
| CAAACATAGACACGCTGCAT+CGG | + | chr3.3:69373827-69373846 | None:intergenic | 45.0% | |
| CAGAAAAGGAAAGGCTCGAT+TGG | + | chr3.3:69375311-69375330 | None:intergenic | 45.0% | |
| CAGCCGAACTTTCTTCGATA+TGG | - | chr3.3:69374061-69374080 | MS.gene25698:intron | 45.0% | |
| CCTTGAATGTCTTCGAGCAA+GGG | + | chr3.3:69373881-69373900 | None:intergenic | 45.0% | |
| GAAAAACGCCTAACAAGGGT+GGG | - | chr3.3:69375588-69375607 | MS.gene25698:CDS | 45.0% | |
| GCTGGAGACTATCAAGATTG+TGG | - | chr3.3:69375351-69375370 | MS.gene25698:intron | 45.0% | |
| GGACAAGTGTTTAACCAAGG+AGG | + | chr3.3:69375405-69375424 | None:intergenic | 45.0% | |
| GTTCACAACCTGCTTTGGTT+AGG | - | chr3.3:69373923-69373942 | MS.gene25698:intron | 45.0% | |
| TCAGAACACCTAACCAAAGC+AGG | + | chr3.3:69373934-69373953 | None:intergenic | 45.0% | |
| TCCTTGAATGTCTTCGAGCA+AGG | + | chr3.3:69373882-69373901 | None:intergenic | 45.0% | |
| TGAAAAACGCCTAACAAGGG+TGG | - | chr3.3:69375587-69375606 | MS.gene25698:CDS | 45.0% | |
| TTGTAGCAGGACAGTATACC+TGG | + | chr3.3:69374561-69374580 | None:intergenic | 45.0% | |
| ! | CAATCGAGCCTTTCCTTTTC+TGG | - | chr3.3:69375309-69375328 | MS.gene25698:intron | 45.0% |
| !! | AAGCACTGGTGAGACTGTTT+GGG | - | chr3.3:69374883-69374902 | MS.gene25698:intron | 45.0% |
| !! | AGCTTCTGGTTTGGCTCAAA+GGG | + | chr3.3:69375979-69375998 | None:intergenic | 45.0% |
| !! | TAAGCACTGGTGAGACTGTT+TGG | - | chr3.3:69374882-69374901 | MS.gene25698:intron | 45.0% |
| !! | TAGCTTCTGGTTTGGCTCAA+AGG | + | chr3.3:69375980-69375999 | None:intergenic | 45.0% |
| !! | TTCGGGCATTGAGAATGTAC+CGG | + | chr3.3:69374250-69374269 | None:intergenic | 45.0% |
| AGTGAGATATGCCTCTCGCA+AGG | - | chr3.3:69375886-69375905 | MS.gene25698:CDS | 50.0% | |
| CATCATCATCGCTTGCGTCA+GGG | + | chr3.3:69374588-69374607 | None:intergenic | 50.0% | |
| CATTGGTAGATCCATCTGGG+CGG | + | chr3.3:69374299-69374318 | None:intergenic | 50.0% | |
| CCAAGATGCTAATGCTGCTG+AGG | - | chr3.3:69374771-69374790 | MS.gene25698:intron | 50.0% | |
| CCCTTGCTCGAAGACATTCA+AGG | - | chr3.3:69373878-69373897 | MS.gene25698:CDS | 50.0% | |
| CCTCAGCAGCATTAGCATCT+TGG | + | chr3.3:69374774-69374793 | None:intergenic | 50.0% | |
| CGCGGTTCTTTGCTTGATTC+GGG | + | chr3.3:69374267-69374286 | None:intergenic | 50.0% | |
| GAAGAAAGTTCGGCTGACGA+AGG | + | chr3.3:69374057-69374076 | None:intergenic | 50.0% | |
| GATATGCCTCTCGCAAGGAA+AGG | - | chr3.3:69375891-69375910 | MS.gene25698:CDS | 50.0% | |
| GTCATAATCATAGGCCTCGC+CGG | + | chr3.3:69375957-69375976 | None:intergenic | 50.0% | |
| TCATCATCATCGCTTGCGTC+AGG | + | chr3.3:69374589-69374608 | None:intergenic | 50.0% | |
| TTCTCATGGGAGAACCTCCT+TGG | - | chr3.3:69375388-69375407 | MS.gene25698:intron | 50.0% | |
| TTGTGGAGATCAGAGGTCGA+TGG | - | chr3.3:69373789-69373808 | MS.gene25698:CDS | 50.0% | |
| !! | AAGGGCCGGTTTGTTAAAGC+CGG | - | chr3.3:69375935-69375954 | MS.gene25698:CDS | 50.0% |
| AGGAAAGGGCTGATGTCCGA+AGG | - | chr3.3:69375906-69375925 | MS.gene25698:CDS | 55.0% | |
| AGTTCGTCCCCACCCTTGTT+AGG | + | chr3.3:69375599-69375618 | None:intergenic | 55.0% | |
| ATAGACACGCTGCATCGGAG+CGG | + | chr3.3:69373822-69373841 | None:intergenic | 55.0% | |
| ATCAGCCCTTTCCTTGCGAG+AGG | + | chr3.3:69375900-69375919 | None:intergenic | 55.0% | |
| GCGTCAGGGCAATTTGTAGC+AGG | + | chr3.3:69374574-69374593 | None:intergenic | 55.0% | |
| !! | CCTCGCCGGCTTTAACAAAC+CGG | + | chr3.3:69375943-69375962 | None:intergenic | 55.0% |
| GATGCTGCAGTGCTGCATGC+TGG | + | chr3.3:69375228-69375247 | None:intergenic | 60.0% | |
| GGGGCAAAAGCCATGTCTGC+TGG | - | chr3.3:69374736-69374755 | MS.gene25698:intron | 60.0% | |
| ! | GATGTCCGAAGGCGCGTGAA+GGG | - | chr3.3:69375917-69375936 | MS.gene25698:CDS | 60.0% |
| ! | TGATGTCCGAAGGCGCGTGA+AGG | - | chr3.3:69375916-69375935 | MS.gene25698:CDS | 60.0% |
| !! | CCGGTTTGTTAAAGCCGGCG+AGG | - | chr3.3:69375940-69375959 | MS.gene25698:CDS | 60.0% |
| CGTATGCTCGACCGCCCAGA+TGG | - | chr3.3:69374285-69374304 | MS.gene25698:CDS | 65.0% | |
| TCTGGGCGGTCGAGCATACG+CGG | + | chr3.3:69374285-69374304 | None:intergenic | 65.0% | |
| ACCGGCCCTTCACGCGCCTT+CGG | + | chr3.3:69375925-69375944 | None:intergenic | 70.0% | |
| ! | TCCGAAGGCGCGTGAAGGGC+CGG | - | chr3.3:69375921-69375940 | MS.gene25698:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 69373769 | 69376003 | 69373769 | ID=MS.gene25698 |
| chr3.3 | mRNA | 69373769 | 69376003 | 69373769 | ID=MS.gene25698.t1;Parent=MS.gene25698 |
| chr3.3 | exon | 69375440 | 69376003 | 69375440 | ID=MS.gene25698.t1.exon1;Parent=MS.gene25698.t1 |
| chr3.3 | CDS | 69375440 | 69376003 | 69375440 | ID=cds.MS.gene25698.t1;Parent=MS.gene25698.t1 |
| chr3.3 | exon | 69374980 | 69375210 | 69374980 | ID=MS.gene25698.t1.exon2;Parent=MS.gene25698.t1 |
| chr3.3 | CDS | 69374980 | 69375210 | 69374980 | ID=cds.MS.gene25698.t1;Parent=MS.gene25698.t1 |
| chr3.3 | exon | 69374270 | 69374580 | 69374270 | ID=MS.gene25698.t1.exon3;Parent=MS.gene25698.t1 |
| chr3.3 | CDS | 69374270 | 69374580 | 69374270 | ID=cds.MS.gene25698.t1;Parent=MS.gene25698.t1 |
| chr3.3 | exon | 69373769 | 69373898 | 69373769 | ID=MS.gene25698.t1.exon4;Parent=MS.gene25698.t1 |
| chr3.3 | CDS | 69373769 | 69373898 | 69373769 | ID=cds.MS.gene25698.t1;Parent=MS.gene25698.t1 |
| Gene Sequence |
| Protein sequence |
