Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70471.t1 | XP_003601522.1 | 99.3 | 411 | 3 | 0 | 1 | 411 | 1 | 411 | 3.20E-198 | 701 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70471.t1 | Q9SSE5 | 55.6 | 423 | 125 | 10 | 1 | 411 | 1 | 372 | 3.0e-80 | 300.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70471.t1 | G7J8U1 | 99.3 | 411 | 3 | 0 | 1 | 411 | 1 | 411 | 2.3e-198 | 701.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene70471.t1 | TF | C2C2-CO-like |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050702 | MS.gene70471 | -0.875109 | 3.99E-68 | -1.69E-46 |
| MS.gene058551 | MS.gene70471 | 0.881035 | 3.35E-70 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene43742 | MS.gene70471 | PPI |
| MS.gene70471 | MS.gene044331 | PPI |
| MS.gene70471 | MS.gene009935 | PPI |
| MS.gene70471 | MS.gene25698 | PPI |
| MS.gene88720 | MS.gene70471 | PPI |
| MS.gene70471 | MS.gene44846 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70471.t1 | MTR_3g082630 | 99.270 | 411 | 3 | 0 | 1 | 411 | 1 | 411 | 0.0 | 846 |
| MS.gene70471.t1 | MTR_5g069480 | 68.916 | 415 | 118 | 7 | 1 | 410 | 1 | 409 | 0.0 | 514 |
| MS.gene70471.t1 | MTR_5g069480 | 65.926 | 270 | 85 | 5 | 1 | 265 | 1 | 268 | 1.17e-117 | 345 |
| MS.gene70471.t1 | MTR_2g088900 | 32.308 | 455 | 224 | 11 | 1 | 405 | 97 | 517 | 1.57e-62 | 210 |
| MS.gene70471.t1 | MTR_7g108150 | 48.544 | 103 | 53 | 0 | 5 | 107 | 20 | 122 | 2.39e-29 | 117 |
| MS.gene70471.t1 | MTR_7g108150 | 62.500 | 48 | 18 | 0 | 348 | 395 | 322 | 369 | 9.63e-12 | 66.6 |
| MS.gene70471.t1 | MTR_1g109350 | 44.860 | 107 | 57 | 1 | 1 | 107 | 1 | 105 | 1.34e-24 | 103 |
| MS.gene70471.t1 | MTR_7g083540 | 35.971 | 139 | 81 | 2 | 1 | 132 | 1 | 138 | 1.21e-21 | 96.3 |
| MS.gene70471.t1 | MTR_7g083540 | 56.061 | 66 | 29 | 0 | 331 | 396 | 318 | 383 | 1.75e-14 | 75.1 |
| MS.gene70471.t1 | MTR_1g013450 | 44.048 | 84 | 46 | 1 | 4 | 86 | 5 | 88 | 6.15e-17 | 81.6 |
| MS.gene70471.t1 | MTR_3g105710 | 39.216 | 102 | 61 | 1 | 4 | 104 | 5 | 106 | 5.48e-16 | 78.2 |
| MS.gene70471.t1 | MTR_3g105710 | 45.833 | 72 | 36 | 1 | 333 | 401 | 208 | 279 | 4.50e-11 | 63.5 |
| MS.gene70471.t1 | MTR_4g128930 | 38.889 | 90 | 54 | 1 | 4 | 92 | 21 | 110 | 5.26e-15 | 76.3 |
| MS.gene70471.t1 | MTR_7g018170 | 41.758 | 91 | 52 | 1 | 5 | 94 | 26 | 116 | 1.82e-14 | 75.1 |
| MS.gene70471.t1 | MTR_4g067320 | 38.542 | 96 | 49 | 2 | 1 | 86 | 1 | 96 | 1.22e-13 | 70.5 |
| MS.gene70471.t1 | MTR_4g067320 | 38.542 | 96 | 49 | 2 | 1 | 86 | 1 | 96 | 1.24e-13 | 70.5 |
| MS.gene70471.t1 | MTR_5g021580 | 38.095 | 84 | 44 | 1 | 1 | 76 | 1 | 84 | 2.54e-11 | 62.8 |
| MS.gene70471.t1 | MTR_5g021580 | 38.095 | 84 | 44 | 1 | 1 | 76 | 1 | 84 | 3.08e-11 | 62.8 |
| MS.gene70471.t1 | MTR_1g023260 | 34.021 | 97 | 53 | 2 | 1 | 86 | 1 | 97 | 4.00e-11 | 62.4 |
| MS.gene70471.t1 | MTR_1g023260 | 34.021 | 97 | 53 | 2 | 1 | 86 | 1 | 97 | 4.03e-11 | 63.5 |
| MS.gene70471.t1 | MTR_4g071200 | 35.052 | 97 | 52 | 2 | 1 | 86 | 1 | 97 | 5.64e-11 | 62.4 |
| MS.gene70471.t1 | MTR_3g113070 | 33.673 | 98 | 53 | 2 | 1 | 86 | 1 | 98 | 8.87e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70471.t1 | AT3G07650 | 52.955 | 423 | 136 | 10 | 1 | 411 | 1 | 372 | 9.05e-143 | 411 |
| MS.gene70471.t1 | AT3G07650 | 52.955 | 423 | 136 | 10 | 1 | 411 | 1 | 372 | 9.05e-143 | 411 |
| MS.gene70471.t1 | AT3G07650 | 52.955 | 423 | 136 | 10 | 1 | 411 | 1 | 372 | 9.05e-143 | 411 |
| MS.gene70471.t1 | AT3G07650 | 52.955 | 423 | 136 | 10 | 1 | 411 | 1 | 372 | 9.05e-143 | 411 |
| MS.gene70471.t1 | AT5G48250 | 53.699 | 419 | 140 | 10 | 1 | 411 | 1 | 373 | 1.32e-140 | 406 |
| MS.gene70471.t1 | AT3G21880 | 34.063 | 411 | 210 | 11 | 5 | 408 | 9 | 365 | 2.15e-61 | 203 |
| MS.gene70471.t1 | AT3G21880 | 34.063 | 411 | 210 | 11 | 5 | 408 | 9 | 365 | 2.15e-61 | 203 |
| MS.gene70471.t1 | AT3G21880 | 34.063 | 411 | 210 | 11 | 5 | 408 | 5 | 361 | 2.22e-61 | 202 |
| MS.gene70471.t1 | AT4G15250 | 53.153 | 111 | 50 | 2 | 5 | 115 | 5 | 113 | 6.74e-34 | 129 |
| MS.gene70471.t1 | AT4G15250 | 51.020 | 98 | 45 | 1 | 307 | 404 | 232 | 326 | 8.54e-20 | 90.1 |
| MS.gene70471.t1 | AT2G33500 | 47.170 | 106 | 55 | 1 | 5 | 109 | 12 | 117 | 2.81e-30 | 120 |
| MS.gene70471.t1 | AT2G33500 | 60.784 | 51 | 20 | 0 | 346 | 396 | 349 | 399 | 1.42e-12 | 69.3 |
| MS.gene70471.t1 | AT2G33500 | 47.170 | 106 | 55 | 1 | 5 | 109 | 12 | 117 | 2.93e-30 | 120 |
| MS.gene70471.t1 | AT2G33500 | 60.784 | 51 | 20 | 0 | 346 | 396 | 348 | 398 | 1.41e-12 | 69.3 |
| MS.gene70471.t1 | AT1G28050 | 39.041 | 146 | 86 | 2 | 5 | 147 | 9 | 154 | 5.53e-29 | 117 |
| MS.gene70471.t1 | AT1G28050 | 61.111 | 54 | 21 | 0 | 346 | 399 | 377 | 430 | 4.38e-13 | 70.9 |
| MS.gene70471.t1 | AT1G68190 | 46.847 | 111 | 57 | 2 | 1 | 110 | 10 | 119 | 5.74e-29 | 116 |
| MS.gene70471.t1 | AT1G68190 | 46.847 | 111 | 57 | 2 | 1 | 110 | 10 | 119 | 5.74e-29 | 116 |
| MS.gene70471.t1 | AT1G68190 | 46.847 | 111 | 57 | 2 | 1 | 110 | 39 | 148 | 1.01e-28 | 116 |
| MS.gene70471.t1 | AT2G47890 | 35.795 | 176 | 103 | 4 | 4 | 177 | 12 | 179 | 8.74e-26 | 105 |
| MS.gene70471.t1 | AT2G47890 | 36.420 | 162 | 95 | 3 | 4 | 165 | 12 | 165 | 3.16e-25 | 105 |
| MS.gene70471.t1 | AT2G47890 | 36.420 | 162 | 95 | 3 | 4 | 165 | 12 | 165 | 4.04e-25 | 105 |
| MS.gene70471.t1 | AT5G24930 | 43.333 | 90 | 50 | 1 | 4 | 92 | 5 | 94 | 4.07e-17 | 82.8 |
| MS.gene70471.t1 | AT5G24930 | 55.769 | 52 | 23 | 0 | 344 | 395 | 285 | 336 | 3.20e-11 | 64.7 |
| MS.gene70471.t1 | AT2G24790 | 42.222 | 90 | 51 | 1 | 4 | 92 | 7 | 96 | 8.55e-16 | 77.8 |
| MS.gene70471.t1 | AT2G24790 | 50.877 | 57 | 28 | 0 | 347 | 403 | 222 | 278 | 5.16e-11 | 63.5 |
| MS.gene70471.t1 | AT2G24790 | 42.222 | 90 | 51 | 1 | 4 | 92 | 7 | 96 | 8.64e-16 | 76.6 |
| MS.gene70471.t1 | AT5G15840 | 43.956 | 91 | 50 | 1 | 5 | 94 | 20 | 110 | 9.43e-15 | 75.9 |
| MS.gene70471.t1 | AT5G15840 | 43.956 | 91 | 50 | 1 | 5 | 94 | 20 | 110 | 1.59e-14 | 73.9 |
| MS.gene70471.t1 | AT5G15850 | 44.186 | 86 | 47 | 1 | 5 | 89 | 12 | 97 | 2.47e-14 | 74.3 |
| MS.gene70471.t1 | AT3G02380 | 40.000 | 100 | 53 | 2 | 5 | 97 | 16 | 115 | 1.43e-13 | 72.0 |
| MS.gene70471.t1 | AT3G02380 | 62.500 | 48 | 18 | 0 | 348 | 395 | 272 | 319 | 5.23e-12 | 67.0 |
| MS.gene70471.t1 | AT5G57660 | 30.216 | 139 | 92 | 2 | 5 | 142 | 22 | 156 | 2.33e-12 | 68.2 |
| MS.gene70471.t1 | AT2G31380 | 36.458 | 96 | 51 | 2 | 1 | 86 | 1 | 96 | 2.43e-12 | 66.6 |
| MS.gene70471.t1 | AT1G06040 | 37.500 | 96 | 50 | 2 | 1 | 86 | 1 | 96 | 3.90e-12 | 65.1 |
| MS.gene70471.t1 | AT1G06040 | 37.500 | 96 | 50 | 2 | 1 | 86 | 1 | 96 | 4.25e-12 | 66.2 |
| MS.gene70471.t1 | AT2G21320 | 39.286 | 84 | 43 | 1 | 1 | 76 | 1 | 84 | 5.78e-11 | 61.6 |
| MS.gene70471.t1 | AT4G38960 | 36.905 | 84 | 45 | 1 | 1 | 76 | 1 | 84 | 6.07e-11 | 61.6 |
| MS.gene70471.t1 | AT4G38960 | 36.905 | 84 | 45 | 1 | 1 | 76 | 1 | 84 | 6.07e-11 | 61.6 |
| MS.gene70471.t1 | AT1G75540 | 34.343 | 99 | 52 | 2 | 1 | 86 | 1 | 99 | 8.81e-11 | 63.2 |
Find 92 sgRNAs with CRISPR-Local
Find 138 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTGGATTTAACAGTTTATT+TGG | 0.095234 | 3.1:+66676030 | MS.gene70471:CDS |
| AGCTACTGATCTGCCTGATT+TGG | 0.283928 | 3.1:+66675533 | MS.gene70471:CDS |
| GTGGATTTAACAGTTTATTT+GGG | 0.284402 | 3.1:+66676031 | MS.gene70471:CDS |
| CGCGGTTCTTTGCTTGATTC+GGG | 0.298118 | 3.1:-66675585 | None:intergenic |
| TCATCATCATCACTTGCTTC+AGG | 0.322324 | 3.1:-66675901 | None:intergenic |
| ACGCGGTTCTTTGCTTGATT+CGG | 0.327280 | 3.1:-66675586 | None:intergenic |
| CGGCCGAACTTTCTTCGATA+TGG | 0.346888 | 3.1:+66675382 | MS.gene70471:CDS |
| GGATGAAATGGATATAAATA+TGG | 0.347846 | 3.1:+66675948 | MS.gene70471:CDS |
| CGATTTCGATATGGATGAAA+TGG | 0.355447 | 3.1:+66675936 | MS.gene70471:CDS |
| TACCTTTGGTACACATTCAT+TGG | 0.361210 | 3.1:-66675634 | None:intergenic |
| GATGCTGCAGTGCTGCATGC+TGG | 0.368884 | 3.1:-66676540 | None:intergenic |
| TATGTTCAGGGTTCATCAAT+TGG | 0.370264 | 3.1:+66676498 | MS.gene70471:intron |
| ATTATACACTCAGTAGCTTC+TGG | 0.377897 | 3.1:-66677224 | None:intergenic |
| ACCGGCCCTTCACGCGCCTT+CGG | 0.385614 | 3.1:-66677156 | None:intergenic |
| GATCGTTTGCCTCTTATGAT+TGG | 0.395417 | 3.1:-66675334 | None:intergenic |
| CACTTTGTCAGAATTGTGAT+TGG | 0.398736 | 3.1:+66675286 | MS.gene70471:CDS |
| TTTGCTTGATTCGGGCTTTG+AGG | 0.401934 | 3.1:-66675577 | None:intergenic |
| ATGCAGTTCACAACCTGCTT+TGG | 0.404101 | 3.1:+66675239 | MS.gene70471:CDS |
| CCTCAGCAGCATTAGCATCT+TGG | 0.411307 | 3.1:-66676086 | None:intergenic |
| GTTCACAACCTGCTTTGGTT+AGG | 0.412214 | 3.1:+66675244 | MS.gene70471:CDS |
| TAGCTTCTGGTTTGGCTCAA+AGG | 0.415587 | 3.1:-66677211 | None:intergenic |
| ACACTCAGTAGCTTCTGGTT+TGG | 0.416653 | 3.1:-66677219 | None:intergenic |
| CATTCATTGGTAGATCCATC+TGG | 0.421154 | 3.1:-66675621 | None:intergenic |
| AGCTTCTTCAATGCTTCTCA+TGG | 0.422785 | 3.1:+66676689 | MS.gene70471:CDS |
| AGCTTCTGGTTTGGCTCAAA+GGG | 0.425533 | 3.1:-66677210 | None:intergenic |
| CTACATCTTCCAATCATAAG+AGG | 0.437225 | 3.1:+66675325 | MS.gene70471:CDS |
| CAGAAAAGGAAAGGCTCGAT+TGG | 0.437549 | 3.1:-66676623 | None:intergenic |
| GCGAAAGACTTATCCAAATC+AGG | 0.442332 | 3.1:-66675546 | None:intergenic |
| AGCATTAGCATCTTGGCAAT+TGG | 0.442849 | 3.1:-66676079 | None:intergenic |
| TGTCAGAATTGTGATTGGTT+AGG | 0.444655 | 3.1:+66675291 | MS.gene70471:CDS |
| TGTAGTTGTAGCTCATTCTC+AGG | 0.468722 | 3.1:-66676738 | None:intergenic |
| CATCATCATCACTTGCTTCA+GGG | 0.475636 | 3.1:-66675900 | None:intergenic |
| GTCATAATCGTAGGCCTCGC+CGG | 0.482944 | 3.1:-66677188 | None:intergenic |
| TCCGAAGGCGCGTGAAGGGC+CGG | 0.483385 | 3.1:+66677155 | MS.gene70471:CDS |
| TTTGCAGTAATACAAAGATT+CGG | 0.484517 | 3.1:-66676594 | None:intergenic |
| GGGGCAAAAGCCATGTCTGC+TGG | 0.484909 | 3.1:+66676051 | MS.gene70471:CDS |
| TAATGAGAATAGGAGTGCTT+GGG | 0.485947 | 3.1:+66675473 | MS.gene70471:CDS |
| TTCTCATGGGAGAACCTCCT+TGG | 0.487490 | 3.1:+66676703 | MS.gene70471:CDS |
| TCATGAGTATTAATGAGAAT+AGG | 0.491713 | 3.1:+66675463 | MS.gene70471:CDS |
| TCTGTATGGCGATTTCGATA+TGG | 0.492638 | 3.1:+66675927 | MS.gene70471:CDS |
| AGACCATATCGAAGAAAGTT+CGG | 0.494435 | 3.1:-66675385 | None:intergenic |
| GCTTCAGGGCAATTTGTAGC+AGG | 0.495109 | 3.1:-66675886 | None:intergenic |
| AGAAAAGGAAAGGCTCGATT+GGG | 0.501130 | 3.1:-66676622 | None:intergenic |
| TTAATGAGAATAGGAGTGCT+TGG | 0.501567 | 3.1:+66675472 | MS.gene70471:CDS |
| ATTAATGAAGACGGTGGTGC+TGG | 0.505210 | 3.1:+66676648 | MS.gene70471:CDS |
| GTCTTCATTAATACCAGAAA+AGG | 0.513355 | 3.1:-66676637 | None:intergenic |
| CCCTTGCTCGAAGACATTCA+AGG | 0.516832 | 3.1:+66675199 | MS.gene70471:CDS |
| ATATGCCTCTCGCAAGGAAA+GGG | 0.519345 | 3.1:+66677126 | MS.gene70471:CDS |
| CAAACGATCAATTGCTACTC+AGG | 0.524349 | 3.1:+66675348 | MS.gene70471:CDS |
| CCTCGCCGGCTTTAACAAAC+CGG | 0.526175 | 3.1:-66677174 | None:intergenic |
| ACTACATGTGAGCAAGAACT+AGG | 0.526382 | 3.1:+66675438 | MS.gene70471:CDS |
| TTGTGGAGATCAACGGTCCA+TGG | 0.528039 | 3.1:+66675110 | MS.gene70471:CDS |
| TCCTTGAATGTCTTCGAGCA+AGG | 0.533062 | 3.1:-66675200 | None:intergenic |
| TTCGGGCTTTGAGGATGTAC+CGG | 0.533096 | 3.1:-66675568 | None:intergenic |
| AAGGGCCGGTTTGTTAAAGC+CGG | 0.538044 | 3.1:+66677169 | MS.gene70471:CDS |
| CGTATGCTCGACCGCCCAGA+TGG | 0.548257 | 3.1:+66675606 | MS.gene70471:CDS |
| AGATGTCCGAAGGCGCGTGA+AGG | 0.558015 | 3.1:+66677150 | MS.gene70471:CDS |
| AATTGTGATTGGTTAGGTCA+TGG | 0.558365 | 3.1:+66675297 | MS.gene70471:CDS |
| CTACTCAGGTTGCCCTTCGT+CGG | 0.563208 | 3.1:+66675362 | MS.gene70471:CDS |
| ATCTGCCCTTTCCTTGCGAG+AGG | 0.563504 | 3.1:-66677131 | None:intergenic |
| ATTGGAATCTCCAGCAGACA+TGG | 0.566425 | 3.1:-66676061 | None:intergenic |
| GCTTCTTCAATGCTTCTCAT+GGG | 0.566506 | 3.1:+66676690 | MS.gene70471:CDS |
| TCAGAACACCTAACCAAAGC+AGG | 0.567607 | 3.1:-66675252 | None:intergenic |
| GATGTCCGAAGGCGCGTGAA+GGG | 0.569243 | 3.1:+66677151 | MS.gene70471:CDS |
| AAGAAAGTTCGGCCGACGAA+GGG | 0.569995 | 3.1:-66675374 | None:intergenic |
| CATGTAGTTTCACTCAGAGA+AGG | 0.576596 | 3.1:-66675423 | None:intergenic |
| TAGTGCTGTTATGCGATACA+AGG | 0.579883 | 3.1:+66676776 | MS.gene70471:CDS |
| TACCAATGAATGTGTACCAA+AGG | 0.580738 | 3.1:+66675632 | MS.gene70471:CDS |
| CCTTGAATGTCTTCGAGCAA+GGG | 0.583830 | 3.1:-66675199 | None:intergenic |
| GATTTGGATAAGTCTTTCGC+CGG | 0.584307 | 3.1:+66675549 | MS.gene70471:CDS |
| GATATGCCTCTCGCAAGGAA+AGG | 0.586037 | 3.1:+66677125 | MS.gene70471:CDS |
| TGGATTTAACAGTTTATTTG+GGG | 0.588596 | 3.1:+66676032 | MS.gene70471:CDS |
| CATTAATACCAGAAAAGGAA+AGG | 0.592524 | 3.1:-66676632 | None:intergenic |
| GAAGAAAGTTCGGCCGACGA+AGG | 0.593920 | 3.1:-66675375 | None:intergenic |
| ATGTAGTTTCACTCAGAGAA+GGG | 0.599442 | 3.1:-66675422 | None:intergenic |
| CCAAGATGCTAATGCTGCTG+AGG | 0.599606 | 3.1:+66676086 | MS.gene70471:CDS |
| AGGAAAGGGCAGATGTCCGA+AGG | 0.608225 | 3.1:+66677140 | MS.gene70471:CDS |
| GCTCAAAGGGTCATAATCGT+AGG | 0.609995 | 3.1:-66677197 | None:intergenic |
| ATTCATTGGTAGATCCATCT+GGG | 0.610627 | 3.1:-66675620 | None:intergenic |
| AAAGCTCTTCAGAATGAGTG+AGG | 0.617795 | 3.1:-66675999 | None:intergenic |
| AGTGAGATATGCCTCTCGCA+AGG | 0.618457 | 3.1:+66677120 | MS.gene70471:CDS |
| GCTGGAGACTATCAAGATTG+TGG | 0.619737 | 3.1:+66676666 | MS.gene70471:CDS |
| TCTGGTATTAATGAAGACGG+TGG | 0.629157 | 3.1:+66676642 | MS.gene70471:CDS |
| TCAGGACATGTGTTTAACCA+AGG | 0.635746 | 3.1:-66676720 | None:intergenic |
| CCGGTTTGTTAAAGCCGGCG+AGG | 0.638136 | 3.1:+66677174 | MS.gene70471:CDS |
| GGTGTACTGTCGCTCAGATG+CGG | 0.641096 | 3.1:+66675131 | MS.gene70471:CDS |
| TCTGGGCGGTCGAGCATACG+CGG | 0.641340 | 3.1:-66675603 | None:intergenic |
| AAGCTCTTCAGAATGAGTGA+GGG | 0.667176 | 3.1:-66675998 | None:intergenic |
| GGACATGTGTTTAACCAAGG+AGG | 0.698616 | 3.1:-66676717 | None:intergenic |
| ATCGCATAACAGCACTACAG+CGG | 0.734007 | 3.1:-66676770 | None:intergenic |
| ATCTGAGCGACAGTACACCA+TGG | 0.755518 | 3.1:-66675127 | None:intergenic |
| CATTGGTAGATCCATCTGGG+CGG | 0.761162 | 3.1:-66675617 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTAATTTGATAAAAATGTA+AGG | - | chr3.1:66675830-66675849 | None:intergenic | 15.0% |
| !!! | ATTGATTGTTATTATCTTTT+AGG | + | chr3.1:66677086-66677105 | MS.gene70471:intron | 15.0% |
| !!! | AATAAGTTGAAAACAACTTA+TGG | - | chr3.1:66676253-66676272 | None:intergenic | 20.0% |
| !!! | ACATCTAATTTTTATGTTCA+GGG | + | chr3.1:66676486-66676505 | MS.gene70471:intron | 20.0% |
| !!! | TACATCTAATTTTTATGTTC+AGG | + | chr3.1:66676485-66676504 | MS.gene70471:intron | 20.0% |
| !!! | TCAGATCTTATATCTTTTAA+GGG | - | chr3.1:66676911-66676930 | None:intergenic | 20.0% |
| !!! | TTCAGATCTTATATCTTTTA+AGG | - | chr3.1:66676912-66676931 | None:intergenic | 20.0% |
| ! | AACATTCAGATATATACCTT+TGG | - | chr3.1:66675651-66675670 | None:intergenic | 25.0% |
| ! | ATACAAATTGTTTACACCTT+CGG | + | chr3.1:66676435-66676454 | MS.gene70471:intron | 25.0% |
| ! | GGATGAAATGGATATAAATA+TGG | + | chr3.1:66675948-66675967 | MS.gene70471:CDS | 25.0% |
| ! | GTGGATTTAACAGTTTATTT+GGG | + | chr3.1:66676031-66676050 | MS.gene70471:CDS | 25.0% |
| ! | TCATGAGTATTAATGAGAAT+AGG | + | chr3.1:66675463-66675482 | MS.gene70471:CDS | 25.0% |
| ! | TGGATTTAACAGTTTATTTG+GGG | + | chr3.1:66676032-66676051 | MS.gene70471:CDS | 25.0% |
| ! | TTATATATTAGCATAAGCAC+TGG | + | chr3.1:66676184-66676203 | MS.gene70471:intron | 25.0% |
| ! | TTTGCAGTAATACAAAGATT+CGG | - | chr3.1:66676597-66676616 | None:intergenic | 25.0% |
| !!! | TTTTCTGGTATTAATGAAGA+CGG | + | chr3.1:66676639-66676658 | MS.gene70471:CDS | 25.0% |
| ACGCAATCAAATGATAGAAA+AGG | - | chr3.1:66676150-66676169 | None:intergenic | 30.0% | |
| AGAGGAAAAACACATTCATT+AGG | - | chr3.1:66676874-66676893 | None:intergenic | 30.0% | |
| ATGATTAAGCTATTGCTCTA+TGG | - | chr3.1:66675725-66675744 | None:intergenic | 30.0% | |
| CATTAATACCAGAAAAGGAA+AGG | - | chr3.1:66676635-66676654 | None:intergenic | 30.0% | |
| GGATTTCAATTATAAACCTC+AGG | - | chr3.1:66675809-66675828 | None:intergenic | 30.0% | |
| GTCTTCATTAATACCAGAAA+AGG | - | chr3.1:66676640-66676659 | None:intergenic | 30.0% | |
| TAGAGCAATAGCTTAATCAT+TGG | + | chr3.1:66675725-66675744 | MS.gene70471:intron | 30.0% | |
| TGAAAAGAGTTTCTAAACAG+TGG | + | chr3.1:66676928-66676947 | MS.gene70471:intron | 30.0% | |
| TTTCTATCATTTGATTGCGT+TGG | + | chr3.1:66676150-66676169 | MS.gene70471:intron | 30.0% | |
| ! | ATGAATGTGTTTTTCCTCTT+TGG | + | chr3.1:66676875-66676894 | MS.gene70471:intron | 30.0% |
| ! | CTGTTTTCATAAGCTTATCT+TGG | - | chr3.1:66676288-66676307 | None:intergenic | 30.0% |
| ! | GAGAACTATGATGAACTTTT+TGG | + | chr3.1:66675970-66675989 | MS.gene70471:CDS | 30.0% |
| ! | GGTGGATTTAACAGTTTATT+TGG | + | chr3.1:66676030-66676049 | MS.gene70471:CDS | 30.0% |
| ! | GGTTACATATGTGATTTTTG+TGG | + | chr3.1:66675093-66675112 | MS.gene70471:CDS | 30.0% |
| ! | TGAATGTGTTTTTCCTCTTT+GGG | + | chr3.1:66676876-66676895 | MS.gene70471:intron | 30.0% |
| !! | CTATGATGAACTTTTTGGTA+TGG | + | chr3.1:66675975-66675994 | MS.gene70471:CDS | 30.0% |
| !! | TCTGAAGAGCTTTTTGAAAA+TGG | + | chr3.1:66676009-66676028 | MS.gene70471:CDS | 30.0% |
| !! | TTCAGTTTTAGTGCTCAATA+TGG | - | chr3.1:66676574-66676593 | None:intergenic | 30.0% |
| ACAGAGCTTATGTCTACTAT+TGG | + | chr3.1:66676397-66676416 | MS.gene70471:intron | 35.0% | |
| AGACCATATCGAAGAAAGTT+CGG | - | chr3.1:66675388-66675407 | None:intergenic | 35.0% | |
| AGCTTATCTTGGAGAACTTA+TGG | - | chr3.1:66676277-66676296 | None:intergenic | 35.0% | |
| ATGTAGTTTCACTCAGAGAA+GGG | - | chr3.1:66675425-66675444 | None:intergenic | 35.0% | |
| ATTCATTGGTAGATCCATCT+GGG | - | chr3.1:66675623-66675642 | None:intergenic | 35.0% | |
| CACTTTGTCAGAATTGTGAT+TGG | + | chr3.1:66675286-66675305 | MS.gene70471:CDS | 35.0% | |
| CGATTTCGATATGGATGAAA+TGG | + | chr3.1:66675936-66675955 | MS.gene70471:CDS | 35.0% | |
| CTACATCTTCCAATCATAAG+AGG | + | chr3.1:66675325-66675344 | MS.gene70471:CDS | 35.0% | |
| CTCTACTTAAAATCAACCGA+AGG | - | chr3.1:66676454-66676473 | None:intergenic | 35.0% | |
| GCATTACTCACTTTACTAAC+TGG | + | chr3.1:66676950-66676969 | MS.gene70471:intron | 35.0% | |
| TACCAATGAATGTGTACCAA+AGG | + | chr3.1:66675632-66675651 | MS.gene70471:CDS | 35.0% | |
| TACCTTTGGTACACATTCAT+TGG | - | chr3.1:66675637-66675656 | None:intergenic | 35.0% | |
| TAGTAGACATAAGCTCTGTT+TGG | - | chr3.1:66676396-66676415 | None:intergenic | 35.0% | |
| TATGTTCAGGGTTCATCAAT+TGG | + | chr3.1:66676498-66676517 | MS.gene70471:intron | 35.0% | |
| TGCTAACAAGCTAGGAATTA+CGG | + | chr3.1:66677012-66677031 | MS.gene70471:intron | 35.0% | |
| ! | AATTGTGATTGGTTAGGTCA+TGG | + | chr3.1:66675297-66675316 | MS.gene70471:CDS | 35.0% |
| ! | GATGATGATGATGATCTGTA+TGG | + | chr3.1:66675913-66675932 | MS.gene70471:CDS | 35.0% |
| ! | GTGATTTTTGTGGAGATCAA+CGG | + | chr3.1:66675103-66675122 | MS.gene70471:CDS | 35.0% |
| ! | TCAGACACGTTCTGATTTTT+CGG | - | chr3.1:66675504-66675523 | None:intergenic | 35.0% |
| ! | TGTCAGAATTGTGATTGGTT+AGG | + | chr3.1:66675291-66675310 | MS.gene70471:CDS | 35.0% |
| !! | AACTCTCATTGTTGATTTCC+AGG | + | chr3.1:66675855-66675874 | MS.gene70471:intron | 35.0% |
| !! | GAAGAGCTTTTTGAAAATGG+TGG | + | chr3.1:66676012-66676031 | MS.gene70471:CDS | 35.0% |
| !! | TAATGAGAATAGGAGTGCTT+GGG | + | chr3.1:66675473-66675492 | MS.gene70471:CDS | 35.0% |
| !! | TTAATGAGAATAGGAGTGCT+TGG | + | chr3.1:66675472-66675491 | MS.gene70471:CDS | 35.0% |
| AAAGCTCTTCAGAATGAGTG+AGG | - | chr3.1:66676002-66676021 | None:intergenic | 40.0% | |
| AAGCTCTTCAGAATGAGTGA+GGG | - | chr3.1:66676001-66676020 | None:intergenic | 40.0% | |
| ACTACATGTGAGCAAGAACT+AGG | + | chr3.1:66675438-66675457 | MS.gene70471:CDS | 40.0% | |
| AGAAAAGGAAAGGCTCGATT+GGG | - | chr3.1:66676625-66676644 | None:intergenic | 40.0% | |
| AGCATTAGCATCTTGGCAAT+TGG | - | chr3.1:66676082-66676101 | None:intergenic | 40.0% | |
| AGCTTCTTCAATGCTTCTCA+TGG | + | chr3.1:66676689-66676708 | MS.gene70471:CDS | 40.0% | |
| CAAACGATCAATTGCTACTC+AGG | + | chr3.1:66675348-66675367 | MS.gene70471:CDS | 40.0% | |
| CATCATCATCACTTGCTTCA+GGG | - | chr3.1:66675903-66675922 | None:intergenic | 40.0% | |
| CATGTAGTTTCACTCAGAGA+AGG | - | chr3.1:66675426-66675445 | None:intergenic | 40.0% | |
| CATTCATTGGTAGATCCATC+TGG | - | chr3.1:66675624-66675643 | None:intergenic | 40.0% | |
| GATCGTTTGCCTCTTATGAT+TGG | - | chr3.1:66675337-66675356 | None:intergenic | 40.0% | |
| GATTTGGATAAGTCTTTCGC+CGG | + | chr3.1:66675549-66675568 | MS.gene70471:CDS | 40.0% | |
| GCGAAAGACTTATCCAAATC+AGG | - | chr3.1:66675549-66675568 | None:intergenic | 40.0% | |
| GCTAACAAGCTAGGAATTAC+GGG | + | chr3.1:66677013-66677032 | MS.gene70471:intron | 40.0% | |
| GCTTCTTCAATGCTTCTCAT+GGG | + | chr3.1:66676690-66676709 | MS.gene70471:CDS | 40.0% | |
| TAAACGAGTTTCACTACCTG+AGG | + | chr3.1:66675790-66675809 | MS.gene70471:intron | 40.0% | |
| TCAGGACATGTGTTTAACCA+AGG | - | chr3.1:66676723-66676742 | None:intergenic | 40.0% | |
| TCATCATCATCACTTGCTTC+AGG | - | chr3.1:66675904-66675923 | None:intergenic | 40.0% | |
| TCTGGTATTAATGAAGACGG+TGG | + | chr3.1:66676642-66676661 | MS.gene70471:CDS | 40.0% | |
| TCTGTATGGCGATTTCGATA+TGG | + | chr3.1:66675927-66675946 | MS.gene70471:CDS | 40.0% | |
| TGTAGTTGTAGCTCATTCTC+AGG | - | chr3.1:66676741-66676760 | None:intergenic | 40.0% | |
| ! | TAGTGCTGTTATGCGATACA+AGG | + | chr3.1:66676776-66676795 | MS.gene70471:CDS | 40.0% |
| !! | TCCTAGCTTGTTAGCAAAGA+CGG | - | chr3.1:66677008-66677027 | None:intergenic | 40.0% |
| ACCGTCTTTGCTAACAAGCT+AGG | + | chr3.1:66677004-66677023 | MS.gene70471:intron | 45.0% | |
| ACGCGGTTCTTTGCTTGATT+CGG | - | chr3.1:66675589-66675608 | None:intergenic | 45.0% | |
| AGACTGTTTGGGAGAGCTTA+TGG | + | chr3.1:66676209-66676228 | MS.gene70471:intron | 45.0% | |
| AGCTACTGATCTGCCTGATT+TGG | + | chr3.1:66675533-66675552 | MS.gene70471:CDS | 45.0% | |
| AGCTTAATCATTGGCGAAGC+AGG | + | chr3.1:66675734-66675753 | MS.gene70471:intron | 45.0% | |
| AGGGAAGTTCATTCCCAAAG+AGG | - | chr3.1:66676892-66676911 | None:intergenic | 45.0% | |
| ATATGCCTCTCGCAAGGAAA+GGG | + | chr3.1:66677126-66677145 | MS.gene70471:CDS | 45.0% | |
| ATCGCATAACAGCACTACAG+CGG | - | chr3.1:66676773-66676792 | None:intergenic | 45.0% | |
| ATGCAGTTCACAACCTGCTT+TGG | + | chr3.1:66675239-66675258 | MS.gene70471:CDS | 45.0% | |
| ATTAATGAAGACGGTGGTGC+TGG | + | chr3.1:66676648-66676667 | MS.gene70471:CDS | 45.0% | |
| ATTGGAATCTCCAGCAGACA+TGG | - | chr3.1:66676064-66676083 | None:intergenic | 45.0% | |
| CAGAAAAGGAAAGGCTCGAT+TGG | - | chr3.1:66676626-66676645 | None:intergenic | 45.0% | |
| CCTTGAATGTCTTCGAGCAA+GGG | - | chr3.1:66675202-66675221 | None:intergenic | 45.0% | |
| GCTCAAAGGGTCATAATCGT+AGG | - | chr3.1:66677200-66677219 | None:intergenic | 45.0% | |
| GCTGGAGACTATCAAGATTG+TGG | + | chr3.1:66676666-66676685 | MS.gene70471:CDS | 45.0% | |
| GGACATGTGTTTAACCAAGG+AGG | - | chr3.1:66676720-66676739 | None:intergenic | 45.0% | |
| GTTCACAACCTGCTTTGGTT+AGG | + | chr3.1:66675244-66675263 | MS.gene70471:CDS | 45.0% | |
| TCAGAACACCTAACCAAAGC+AGG | - | chr3.1:66675255-66675274 | None:intergenic | 45.0% | |
| TCCTTGAATGTCTTCGAGCA+AGG | - | chr3.1:66675203-66675222 | None:intergenic | 45.0% | |
| TTGTAGCAGGACAGTATACC+TGG | - | chr3.1:66675876-66675895 | None:intergenic | 45.0% | |
| ! | CAATCGAGCCTTTCCTTTTC+TGG | + | chr3.1:66676624-66676643 | MS.gene70471:CDS | 45.0% |
| !! | AAGCACTGGTGAGACTGTTT+GGG | + | chr3.1:66676198-66676217 | MS.gene70471:intron | 45.0% |
| !! | AGCTTCTGGTTTGGCTCAAA+GGG | - | chr3.1:66677213-66677232 | None:intergenic | 45.0% |
| !! | TAAGCACTGGTGAGACTGTT+TGG | + | chr3.1:66676197-66676216 | MS.gene70471:intron | 45.0% |
| !! | TAGCTTCTGGTTTGGCTCAA+AGG | - | chr3.1:66677214-66677233 | None:intergenic | 45.0% |
| !! | TTTGCTTGATTCGGGCTTTG+AGG | - | chr3.1:66675580-66675599 | None:intergenic | 45.0% |
| AAGAAAGTTCGGCCGACGAA+GGG | - | chr3.1:66675377-66675396 | None:intergenic | 50.0% | |
| AGTGAGATATGCCTCTCGCA+AGG | + | chr3.1:66677120-66677139 | MS.gene70471:CDS | 50.0% | |
| ATCTGAGCGACAGTACACCA+TGG | - | chr3.1:66675130-66675149 | None:intergenic | 50.0% | |
| CATTGGTAGATCCATCTGGG+CGG | - | chr3.1:66675620-66675639 | None:intergenic | 50.0% | |
| CCAAGATGCTAATGCTGCTG+AGG | + | chr3.1:66676086-66676105 | MS.gene70471:CDS | 50.0% | |
| CCCTTGCTCGAAGACATTCA+AGG | + | chr3.1:66675199-66675218 | MS.gene70471:CDS | 50.0% | |
| CCTCAGCAGCATTAGCATCT+TGG | - | chr3.1:66676089-66676108 | None:intergenic | 50.0% | |
| CGCGGTTCTTTGCTTGATTC+GGG | - | chr3.1:66675588-66675607 | None:intergenic | 50.0% | |
| CGGCCGAACTTTCTTCGATA+TGG | + | chr3.1:66675382-66675401 | MS.gene70471:CDS | 50.0% | |
| GATATGCCTCTCGCAAGGAA+AGG | + | chr3.1:66677125-66677144 | MS.gene70471:CDS | 50.0% | |
| GCTTCAGGGCAATTTGTAGC+AGG | - | chr3.1:66675889-66675908 | None:intergenic | 50.0% | |
| TTCTCATGGGAGAACCTCCT+TGG | + | chr3.1:66676703-66676722 | MS.gene70471:CDS | 50.0% | |
| TTGTGGAGATCAACGGTCCA+TGG | + | chr3.1:66675110-66675129 | MS.gene70471:CDS | 50.0% | |
| !! | AAGGGCCGGTTTGTTAAAGC+CGG | + | chr3.1:66677169-66677188 | MS.gene70471:CDS | 50.0% |
| !! | TTCGGGCTTTGAGGATGTAC+CGG | - | chr3.1:66675571-66675590 | None:intergenic | 50.0% |
| AGGAAAGGGCAGATGTCCGA+AGG | + | chr3.1:66677140-66677159 | MS.gene70471:CDS | 55.0% | |
| ATCTGCCCTTTCCTTGCGAG+AGG | - | chr3.1:66677134-66677153 | None:intergenic | 55.0% | |
| GAAGAAAGTTCGGCCGACGA+AGG | - | chr3.1:66675378-66675397 | None:intergenic | 55.0% | |
| GGTGTACTGTCGCTCAGATG+CGG | + | chr3.1:66675131-66675150 | MS.gene70471:CDS | 55.0% | |
| GTCATAATCGTAGGCCTCGC+CGG | - | chr3.1:66677191-66677210 | None:intergenic | 55.0% | |
| ! | CTACTCAGGTTGCCCTTCGT+CGG | + | chr3.1:66675362-66675381 | MS.gene70471:CDS | 55.0% |
| !! | CCTCGCCGGCTTTAACAAAC+CGG | - | chr3.1:66677177-66677196 | None:intergenic | 55.0% |
| GATGCTGCAGTGCTGCATGC+TGG | - | chr3.1:66676543-66676562 | None:intergenic | 60.0% | |
| GGGGCAAAAGCCATGTCTGC+TGG | + | chr3.1:66676051-66676070 | MS.gene70471:CDS | 60.0% | |
| ! | AGATGTCCGAAGGCGCGTGA+AGG | + | chr3.1:66677150-66677169 | MS.gene70471:CDS | 60.0% |
| ! | GATGTCCGAAGGCGCGTGAA+GGG | + | chr3.1:66677151-66677170 | MS.gene70471:CDS | 60.0% |
| !! | CCGGTTTGTTAAAGCCGGCG+AGG | + | chr3.1:66677174-66677193 | MS.gene70471:CDS | 60.0% |
| CGTATGCTCGACCGCCCAGA+TGG | + | chr3.1:66675606-66675625 | MS.gene70471:CDS | 65.0% | |
| TCTGGGCGGTCGAGCATACG+CGG | - | chr3.1:66675606-66675625 | None:intergenic | 65.0% | |
| ACCGGCCCTTCACGCGCCTT+CGG | - | chr3.1:66677159-66677178 | None:intergenic | 70.0% | |
| ! | TCCGAAGGCGCGTGAAGGGC+CGG | + | chr3.1:66677155-66677174 | MS.gene70471:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 66675090 | 66677237 | 66675090 | ID=MS.gene70471 |
| chr3.1 | mRNA | 66675090 | 66677237 | 66675090 | ID=MS.gene70471.t1;Parent=MS.gene70471 |
| chr3.1 | exon | 66675090 | 66675653 | 66675090 | ID=MS.gene70471.t1.exon1;Parent=MS.gene70471.t1 |
| chr3.1 | CDS | 66675090 | 66675653 | 66675090 | ID=cds.MS.gene70471.t1;Parent=MS.gene70471.t1 |
| chr3.1 | exon | 66675877 | 66676107 | 66675877 | ID=MS.gene70471.t1.exon2;Parent=MS.gene70471.t1 |
| chr3.1 | CDS | 66675877 | 66676107 | 66675877 | ID=cds.MS.gene70471.t1;Parent=MS.gene70471.t1 |
| chr3.1 | exon | 66676507 | 66676817 | 66676507 | ID=MS.gene70471.t1.exon3;Parent=MS.gene70471.t1 |
| chr3.1 | CDS | 66676507 | 66676817 | 66676507 | ID=cds.MS.gene70471.t1;Parent=MS.gene70471.t1 |
| chr3.1 | exon | 66677108 | 66677237 | 66677108 | ID=MS.gene70471.t1.exon4;Parent=MS.gene70471.t1 |
| chr3.1 | CDS | 66677108 | 66677237 | 66677108 | ID=cds.MS.gene70471.t1;Parent=MS.gene70471.t1 |
| Gene Sequence |
| Protein sequence |
