Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene045655.t1 | RHN59829.1 | 99.5 | 397 | 2 | 0 | 1 | 397 | 1 | 397 | 1.20E-215 | 758.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene045655.t1 | Q8L5U0 | 84.1 | 397 | 63 | 0 | 1 | 397 | 1 | 397 | 7.4e-185 | 647.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene045655.t1 | A0A396I2I5 | 99.5 | 397 | 2 | 0 | 1 | 397 | 1 | 397 | 8.9e-216 | 758.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene017884 | MS.gene045655 | PPI |
| MS.gene018310 | MS.gene045655 | PPI |
| MS.gene39043 | MS.gene045655 | PPI |
| MS.gene045655 | MS.gene86150 | PPI |
| MS.gene42526 | MS.gene045655 | PPI |
| MS.gene014112 | MS.gene045655 | PPI |
| MS.gene018309 | MS.gene045655 | PPI |
| MS.gene37293 | MS.gene045655 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene045655.t1 | MTR_0173s0030 | 99.244 | 397 | 3 | 0 | 1 | 397 | 1 | 397 | 0.0 | 802 |
| MS.gene045655.t1 | MTR_2g034320 | 46.591 | 88 | 28 | 4 | 97 | 183 | 20 | 89 | 5.23e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene045655.t1 | AT5G42970 | 84.131 | 397 | 63 | 0 | 1 | 397 | 1 | 397 | 0.0 | 686 |
Find 76 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTATATATCAGGTGTATT+TGG | 0.162807 | 4.3:-64021308 | MS.gene045655:intron |
| TCTGCAAAAGCATCAATTTC+AGG | 0.220902 | 4.3:+64021268 | None:intergenic |
| TTTGGTTATTAGAGAGAAAT+TGG | 0.222768 | 4.3:-64024465 | MS.gene045655:CDS |
| ACTGTTCTATCTTCTGTCTT+TGG | 0.232195 | 4.3:+64026926 | None:intergenic |
| CAAATTGAGAAAAGGCAAAT+AGG | 0.242371 | 4.3:-64023692 | MS.gene045655:CDS |
| CATTAATAAAGCATCATTCT+TGG | 0.283000 | 4.3:-64023924 | MS.gene045655:CDS |
| GGTCAATACCACTGAGCATC+TGG | 0.308345 | 4.3:+64024402 | None:intergenic |
| TCAAGCTCTAAATGATGTTT+TGG | 0.311421 | 4.3:-64019786 | MS.gene045655:CDS |
| GAGAGAAACCTTCTGCAGTA+TGG | 0.365955 | 4.3:+64021396 | None:intergenic |
| AGATCTGAAGAGGAAGTTCT+TGG | 0.369986 | 4.3:-64023744 | MS.gene045655:CDS |
| AAATTGAGAAAAGGCAAATA+GGG | 0.370512 | 4.3:-64023691 | MS.gene045655:CDS |
| TCCAGACAATTTCACTGTGC+TGG | 0.378815 | 4.3:-64021139 | MS.gene045655:CDS |
| GCAAACTTTATACAAATATC+AGG | 0.384105 | 4.3:-64021080 | MS.gene045655:intron |
| TCTTGTAGCTTTGATGAGTT+AGG | 0.384222 | 4.3:-64020961 | MS.gene045655:intron |
| TGCCGTTACGTGTACAATAT+TGG | 0.385486 | 4.3:-64021581 | MS.gene045655:CDS |
| GGCAATAATCCAAGTCAAAC+AGG | 0.393367 | 4.3:+64019734 | None:intergenic |
| GCAATCTCTTTCTGTGTCTC+AGG | 0.418879 | 4.3:+64026247 | None:intergenic |
| TGCTGAAGAGTTGGGAAGGT+TGG | 0.443905 | 4.3:-64026272 | MS.gene045655:CDS |
| TCCAGCACAGTGAAATTGTC+TGG | 0.443989 | 4.3:+64021138 | None:intergenic |
| AATCCAAGTCAAACAGGAAC+AGG | 0.449263 | 4.3:+64019740 | None:intergenic |
| TGTACAATATTGGCAGGTGC+AGG | 0.482785 | 4.3:-64021571 | MS.gene045655:CDS |
| AGCATCAAGAATGATTTATG+AGG | 0.485288 | 4.3:-64020505 | MS.gene045655:CDS |
| CAGTGAAATTGTCTGGAAGA+AGG | 0.485991 | 4.3:+64021145 | None:intergenic |
| AGTATTGAATTTGAAGTACA+AGG | 0.490159 | 4.3:-64023882 | MS.gene045655:intron |
| TGTATTTGGAAAGAATTCTT+CGG | 0.492392 | 4.3:-64021294 | MS.gene045655:CDS |
| AGTGGTATTGACCTAGATTC+AGG | 0.492697 | 4.3:-64024392 | MS.gene045655:CDS |
| GATGAGTTAGGCACACTGTT+GGG | 0.493284 | 4.3:-64020949 | MS.gene045655:CDS |
| GATTTATGAGGATAGGATGA+AGG | 0.501314 | 4.3:-64020493 | MS.gene045655:CDS |
| GGGTAATTGATGATAAGTTC+AGG | 0.503152 | 4.3:-64024254 | MS.gene045655:CDS |
| AAATTATGTACCTTGGGAGG+TGG | 0.506288 | 4.3:+64020922 | None:intergenic |
| GAAATTAATGAGGAAGCGCT+TGG | 0.507602 | 4.3:-64021619 | MS.gene045655:CDS |
| ACACTGAAGAGTTGCAAAGA+TGG | 0.510433 | 4.3:-64020319 | MS.gene045655:CDS |
| ACATATCTCAAATTGAGAAA+AGG | 0.510694 | 4.3:-64023700 | MS.gene045655:CDS |
| GATGAAGGGATCTATTGATC+AGG | 0.510987 | 4.3:-64020478 | MS.gene045655:intron |
| AGAAGAATTGAAACCACATC+AGG | 0.513961 | 4.3:-64021248 | MS.gene045655:intron |
| TGTTCTTGCCACACTGTACA+AGG | 0.521177 | 4.3:-64021533 | MS.gene045655:intron |
| GAGACCTCATTCCTGAATCT+AGG | 0.525371 | 4.3:+64024381 | None:intergenic |
| AGTGAAATTGTCTGGAAGAA+GGG | 0.526038 | 4.3:+64021146 | None:intergenic |
| AGAGAAACCTTCTGCAGTAT+GGG | 0.529187 | 4.3:+64021397 | None:intergenic |
| TTGGATAGCATGGGCAAGAA+GGG | 0.533962 | 4.3:-64019767 | MS.gene045655:CDS |
| CAAACTTTAATAACCTGATG+TGG | 0.534249 | 4.3:+64021235 | None:intergenic |
| AAATTAATGAGGAAGCGCTT+GGG | 0.534615 | 4.3:-64021618 | MS.gene045655:CDS |
| CACTGAAGAGTTGCAAAGAT+GGG | 0.536188 | 4.3:-64020318 | MS.gene045655:CDS |
| AATTGCTTGTTTATACCTTG+AGG | 0.536370 | 4.3:-64024214 | MS.gene045655:intron |
| TCAAATTCAATACTTCATGC+TGG | 0.537152 | 4.3:+64023891 | None:intergenic |
| GTTGCAAAGATGGGACCAGC+AGG | 0.537433 | 4.3:-64020309 | MS.gene045655:intron |
| TTTGGATAGCATGGGCAAGA+AGG | 0.544420 | 4.3:-64019768 | MS.gene045655:CDS |
| TGATGAGTTAGGCACACTGT+TGG | 0.554252 | 4.3:-64020950 | MS.gene045655:CDS |
| CAAATGACATTCTTCAAGCT+AGG | 0.557041 | 4.3:-64026873 | MS.gene045655:CDS |
| GGCTGAGCTTTATGAATCTG+AGG | 0.561921 | 4.3:-64024444 | MS.gene045655:CDS |
| AGCTGACGCGACACTACTAA+AGG | 0.562961 | 4.3:+64026307 | None:intergenic |
| CAAGAATGATTTATGAGGAT+AGG | 0.564825 | 4.3:-64020500 | MS.gene045655:CDS |
| GATCTATCCCATACTGCAGA+AGG | 0.565134 | 4.3:-64021404 | MS.gene045655:intron |
| ATATTACAGGGAAATTAATG+AGG | 0.565776 | 4.3:-64021629 | MS.gene045655:intron |
| AATTATGTACCTTGGGAGGT+GGG | 0.571748 | 4.3:+64020923 | None:intergenic |
| GTCAATACCACTGAGCATCT+GGG | 0.574024 | 4.3:+64024403 | None:intergenic |
| ATTTATGAGGATAGGATGAA+GGG | 0.574286 | 4.3:-64020492 | MS.gene045655:CDS |
| CTAGGATCTTAGATCTGAAG+AGG | 0.577929 | 4.3:-64023754 | MS.gene045655:CDS |
| GTTACGTGTACAATATTGGC+AGG | 0.588398 | 4.3:-64021577 | MS.gene045655:CDS |
| TGTACAGTGTGGCAAGAACA+CGG | 0.592521 | 4.3:+64021536 | None:intergenic |
| CACTACTAAAGGAACATCAT+CGG | 0.592738 | 4.3:+64026318 | None:intergenic |
| TGCCAATATTGTACACGTAA+CGG | 0.598388 | 4.3:+64021579 | None:intergenic |
| TTTATGAATCTGAGGAGCAA+TGG | 0.600846 | 4.3:-64024436 | MS.gene045655:CDS |
| TTGACCTAGATTCAGGAATG+AGG | 0.609769 | 4.3:-64024385 | MS.gene045655:intron |
| GTTGGGAATCCCACCTCCCA+AGG | 0.613288 | 4.3:-64020932 | MS.gene045655:intron |
| GTACAATATTGGCAGGTGCA+GGG | 0.613304 | 4.3:-64021570 | MS.gene045655:CDS |
| GATTATGCTCAATCATAGCT+CGG | 0.614554 | 4.3:+64021115 | None:intergenic |
| AATTGAGAAAAGGCAAATAG+GGG | 0.620222 | 4.3:-64023690 | MS.gene045655:CDS |
| AGATATGTCATAATATCGCA+AGG | 0.635346 | 4.3:+64023715 | None:intergenic |
| AATCTTTACCTTGTACAGTG+TGG | 0.647262 | 4.3:+64021525 | None:intergenic |
| ATGAAATTATGTACCTTGGG+AGG | 0.648793 | 4.3:+64020919 | None:intergenic |
| GTACAGTGTGGCAAGAACAC+GGG | 0.653612 | 4.3:+64021537 | None:intergenic |
| GCAAGAACACGGGAACGTTG+AGG | 0.664537 | 4.3:+64021547 | None:intergenic |
| AAAGCTGCCCAGATGCTCAG+TGG | 0.700066 | 4.3:-64024410 | MS.gene045655:CDS |
| TGATGTGAAACAGTGATGGA+GGG | 0.716084 | 4.3:-64026979 | None:intergenic |
| TCTTCAAAAGACACAACTCG+AGG | 0.728488 | 4.3:+64026199 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 64019748 | 64026986 | 64019748 | ID=MS.gene045655 |
| chr4.3 | mRNA | 64019748 | 64026986 | 64019748 | ID=MS.gene045655.t1;Parent=MS.gene045655 |
| chr4.3 | exon | 64026857 | 64026986 | 64026857 | ID=MS.gene045655.t1.exon1;Parent=MS.gene045655.t1 |
| chr4.3 | CDS | 64026857 | 64026986 | 64026857 | ID=cds.MS.gene045655.t1;Parent=MS.gene045655.t1 |
| chr4.3 | exon | 64026195 | 64026346 | 64026195 | ID=MS.gene045655.t1.exon2;Parent=MS.gene045655.t1 |
| chr4.3 | CDS | 64026195 | 64026346 | 64026195 | ID=cds.MS.gene045655.t1;Parent=MS.gene045655.t1 |
| chr4.3 | exon | 64024386 | 64024489 | 64024386 | ID=MS.gene045655.t1.exon3;Parent=MS.gene045655.t1 |
| chr4.3 | CDS | 64024386 | 64024489 | 64024386 | ID=cds.MS.gene045655.t1;Parent=MS.gene045655.t1 |
| chr4.3 | exon | 64024215 | 64024275 | 64024215 | ID=MS.gene045655.t1.exon4;Parent=MS.gene045655.t1 |
| chr4.3 | CDS | 64024215 | 64024275 | 64024215 | ID=cds.MS.gene045655.t1;Parent=MS.gene045655.t1 |
| chr4.3 | exon | 64023883 | 64023975 | 64023883 | ID=MS.gene045655.t1.exon5;Parent=MS.gene045655.t1 |
| chr4.3 | CDS | 64023883 | 64023975 | 64023883 | ID=cds.MS.gene045655.t1;Parent=MS.gene045655.t1 |
| chr4.3 | exon | 64023686 | 64023786 | 64023686 | ID=MS.gene045655.t1.exon6;Parent=MS.gene045655.t1 |
| chr4.3 | CDS | 64023686 | 64023786 | 64023686 | ID=cds.MS.gene045655.t1;Parent=MS.gene045655.t1 |
| chr4.3 | exon | 64021534 | 64021642 | 64021534 | ID=MS.gene045655.t1.exon7;Parent=MS.gene045655.t1 |
| chr4.3 | CDS | 64021534 | 64021642 | 64021534 | ID=cds.MS.gene045655.t1;Parent=MS.gene045655.t1 |
| chr4.3 | exon | 64021405 | 64021449 | 64021405 | ID=MS.gene045655.t1.exon8;Parent=MS.gene045655.t1 |
| chr4.3 | CDS | 64021405 | 64021449 | 64021405 | ID=cds.MS.gene045655.t1;Parent=MS.gene045655.t1 |
| chr4.3 | exon | 64021249 | 64021317 | 64021249 | ID=MS.gene045655.t1.exon9;Parent=MS.gene045655.t1 |
| chr4.3 | CDS | 64021249 | 64021317 | 64021249 | ID=cds.MS.gene045655.t1;Parent=MS.gene045655.t1 |
| chr4.3 | exon | 64021081 | 64021172 | 64021081 | ID=MS.gene045655.t1.exon10;Parent=MS.gene045655.t1 |
| chr4.3 | CDS | 64021081 | 64021172 | 64021081 | ID=cds.MS.gene045655.t1;Parent=MS.gene045655.t1 |
| chr4.3 | exon | 64020933 | 64020975 | 64020933 | ID=MS.gene045655.t1.exon11;Parent=MS.gene045655.t1 |
| chr4.3 | CDS | 64020933 | 64020975 | 64020933 | ID=cds.MS.gene045655.t1;Parent=MS.gene045655.t1 |
| chr4.3 | exon | 64020479 | 64020538 | 64020479 | ID=MS.gene045655.t1.exon12;Parent=MS.gene045655.t1 |
| chr4.3 | CDS | 64020479 | 64020538 | 64020479 | ID=cds.MS.gene045655.t1;Parent=MS.gene045655.t1 |
| chr4.3 | exon | 64020310 | 64020369 | 64020310 | ID=MS.gene045655.t1.exon13;Parent=MS.gene045655.t1 |
| chr4.3 | CDS | 64020310 | 64020369 | 64020310 | ID=cds.MS.gene045655.t1;Parent=MS.gene045655.t1 |
| chr4.3 | exon | 64019748 | 64019822 | 64019748 | ID=MS.gene045655.t1.exon14;Parent=MS.gene045655.t1 |
| chr4.3 | CDS | 64019748 | 64019822 | 64019748 | ID=cds.MS.gene045655.t1;Parent=MS.gene045655.t1 |
| Gene Sequence |
| Protein sequence |
