AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene045661

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene045661.t1 MTR_4g035980 97.572 453 10 1 1 453 88 539 0.0 923
MS.gene045661.t1 MTR_3g008640 70.227 440 120 5 16 453 97 527 0.0 637
MS.gene045661.t1 MTR_3g008720 69.773 440 122 5 16 453 97 527 0.0 635
MS.gene045661.t1 MTR_3g033690 67.857 448 132 5 7 453 90 526 0.0 629
MS.gene045661.t1 MTR_3g008700 69.318 440 124 5 16 453 97 527 0.0 628
MS.gene045661.t1 MTR_7g053360 68.409 440 128 5 16 453 97 527 0.0 625
MS.gene045661.t1 MTR_4g025690 54.318 440 183 8 14 453 92 513 7.90e-161 464
MS.gene045661.t1 MTR_4g025730 51.701 441 190 9 14 453 91 509 6.85e-151 439
MS.gene045661.t1 MTR_8g104655 47.414 464 214 8 8 453 90 541 4.33e-149 435
MS.gene045661.t1 MTR_7g108905 49.213 445 204 8 20 449 115 552 2.54e-138 408
MS.gene045661.t1 MTR_8g104645 54.094 342 152 2 116 453 32 372 1.67e-134 392
MS.gene045661.t1 MTR_7g050980 47.248 436 222 5 21 453 125 555 1.55e-132 394
MS.gene045661.t1 MTR_4g025670 47.758 446 198 11 20 453 67 489 1.70e-132 391
MS.gene045661.t1 MTR_8g023310 46.336 464 225 8 11 453 129 589 2.83e-131 391
MS.gene045661.t1 MTR_3g091380 46.053 456 218 8 21 453 147 597 8.95e-129 385
MS.gene045661.t1 MTR_7g050870 46.101 436 227 5 21 453 124 554 1.48e-128 383
MS.gene045661.t1 MTR_1g008140 44.206 466 229 9 16 453 145 607 7.33e-126 378
MS.gene045661.t1 MTR_4g087980 45.598 443 216 8 21 453 89 516 3.37e-125 374
MS.gene045661.t1 MTR_2g044880 48.148 405 200 6 50 453 130 525 1.94e-124 372
MS.gene045661.t1 MTR_7g050950 43.678 435 236 6 22 453 125 553 4.84e-122 367
MS.gene045661.t1 MTR_8g104580 42.241 464 205 9 8 453 82 500 3.24e-120 360
MS.gene045661.t1 MTR_7g112580 43.545 457 231 9 11 453 99 542 6.98e-119 358
MS.gene045661.t1 MTR_7g108900 41.770 486 241 9 4 453 81 560 6.16e-118 356
MS.gene045661.t1 MTR_1g088020 43.154 482 229 10 9 453 82 555 8.35e-118 356
MS.gene045661.t1 MTR_1g054880 53.474 331 147 4 128 453 230 558 5.41e-117 354
MS.gene045661.t1 MTR_2g044810 42.105 456 249 8 1 453 91 534 1.81e-116 352
MS.gene045661.t1 MTR_8g042910 43.284 469 213 12 20 453 162 612 1.96e-116 354
MS.gene045661.t1 MTR_4g087970 42.506 447 228 8 21 453 86 517 1.12e-115 349
MS.gene045661.t1 MTR_1g083520 44.469 452 222 11 18 453 90 528 1.13e-114 347
MS.gene045661.t1 MTR_7g108890 51.964 331 152 4 128 453 234 562 3.38e-113 344
MS.gene045661.t1 MTR_3g010780 42.601 446 231 10 20 453 142 574 4.50e-113 344
MS.gene045661.t1 MTR_1g088225 42.678 478 235 11 9 453 82 553 2.96e-112 342
MS.gene045661.t1 MTR_4g124520 42.282 447 244 9 12 453 84 521 1.36e-111 339
MS.gene045661.t1 MTR_7g108875 41.505 465 238 9 20 453 103 564 2.12e-111 340
MS.gene045661.t1 MTR_3g010770 50.893 336 157 4 124 453 242 575 7.81e-111 338
MS.gene045661.t1 MTR_0458s0030 58.123 277 112 3 179 453 40 314 4.84e-110 327
MS.gene045661.t1 MTR_1g088040 58.123 277 112 3 179 453 40 314 4.84e-110 327
MS.gene045661.t1 MTR_8g042900 43.439 442 231 10 21 453 150 581 7.43e-110 336
MS.gene045661.t1 MTR_4g124550 39.738 458 256 8 12 453 140 593 5.76e-105 324
MS.gene045661.t1 MTR_0618s0020 60.526 228 89 1 226 453 2 228 2.54e-101 302
MS.gene045661.t1 MTR_8g023150 41.031 446 242 10 22 453 144 582 1.15e-100 312
MS.gene045661.t1 MTR_1g083510 39.186 467 240 13 22 452 124 582 1.41e-96 302
MS.gene045661.t1 MTR_1g086210 43.503 354 191 4 107 453 218 569 4.27e-96 300
MS.gene045661.t1 MTR_1g083470 39.659 411 229 8 52 449 162 566 4.55e-96 301
MS.gene045661.t1 MTR_1g083500 39.659 411 229 8 52 449 162 566 4.55e-96 301
MS.gene045661.t1 MTR_1g086190 37.792 471 261 10 7 453 101 563 3.59e-94 295
MS.gene045661.t1 MTR_1g083490 38.702 447 236 11 19 449 147 571 5.48e-94 295
MS.gene045661.t1 MTR_4g005170 38.255 447 238 11 19 449 147 571 1.60e-92 293
MS.gene045661.t1 MTR_7g112590 39.161 429 226 12 52 452 161 582 5.11e-92 290
MS.gene045661.t1 MTR_4g005180 37.079 445 246 9 19 449 129 553 6.89e-92 289
MS.gene045661.t1 MTR_4g120280 36.905 420 247 8 48 452 155 571 5.89e-90 285
MS.gene045661.t1 MTR_4g120290 36.105 421 249 9 48 452 155 571 2.07e-89 283
MS.gene045661.t1 MTR_2g436040 35.358 461 276 12 1 450 94 543 7.84e-89 283
MS.gene045661.t1 MTR_2g044810 52.549 255 119 2 199 453 213 465 9.16e-89 278
MS.gene045661.t1 MTR_2g436050 36.564 454 253 12 22 452 122 563 9.17e-89 281
MS.gene045661.t1 MTR_8g088610 41.141 333 191 3 119 450 45 373 1.38e-85 267
MS.gene045661.t1 MTR_2g044660 45.223 314 165 6 140 453 326 632 1.48e-85 275
MS.gene045661.t1 MTR_5g083620 39.481 347 198 5 113 453 16 356 4.15e-85 265
MS.gene045661.t1 MTR_7g110490 38.228 429 232 8 50 448 144 569 1.98e-83 268
MS.gene045661.t1 MTR_7g110510 38.228 429 232 8 50 448 144 569 1.98e-83 268
MS.gene045661.t1 MTR_8g093460 40.000 415 223 11 50 445 136 543 2.33e-83 267
MS.gene045661.t1 MTR_7g110560 41.279 344 195 3 114 453 209 549 6.29e-82 263
MS.gene045661.t1 MTR_7g110440 38.106 433 237 9 50 453 144 574 1.21e-81 263
MS.gene045661.t1 MTR_3g095920 37.500 472 244 15 13 452 111 563 1.45e-81 262
MS.gene045661.t1 MTR_2g105560 40.000 315 173 5 140 453 40 339 9.72e-75 238
MS.gene045661.t1 MTR_7g107180 39.557 316 177 5 139 453 5 307 3.03e-74 235
MS.gene045661.t1 MTR_7g110420 38.701 354 202 5 114 453 226 578 4.31e-71 236
MS.gene045661.t1 MTR_7g110430 35.328 351 180 5 114 453 226 540 5.19e-60 205
MS.gene045661.t1 MTR_7g111560 32.230 453 266 14 20 452 124 555 9.04e-59 202
MS.gene045661.t1 MTR_0458s0010 32.292 480 247 14 9 453 82 518 1.36e-58 201
MS.gene045661.t1 MTR_6g012800 36.140 285 159 5 141 415 6 277 5.01e-55 186
MS.gene045661.t1 MTR_6g088810 34.483 348 193 10 113 442 47 377 4.35e-52 180
MS.gene045661.t1 MTR_7g011530 37.762 286 149 7 143 415 8 277 5.91e-52 178
MS.gene045661.t1 MTR_1g086540 35.404 322 181 7 131 442 40 344 8.18e-52 178
MS.gene045661.t1 MTR_2g039530 35.897 312 182 8 112 420 23 319 1.22e-51 177
MS.gene045661.t1 MTR_6g024165 34.395 314 174 8 141 442 41 334 2.00e-51 177
MS.gene045661.t1 MTR_8g102380 33.693 371 199 14 86 442 40 377 7.02e-51 177
MS.gene045661.t1 MTR_7g092780 33.750 320 188 8 133 442 82 387 1.37e-45 163
MS.gene045661.t1 MTR_5g083590 30.408 319 169 5 136 453 2 268 1.59e-44 157
MS.gene045661.t1 MTR_7g102600 34.091 308 180 8 143 442 101 393 4.32e-43 156
MS.gene045661.t1 MTR_5g093970 31.908 304 188 6 141 442 43 329 1.64e-42 153
MS.gene045661.t1 MTR_5g095490 34.416 308 173 8 147 442 67 357 3.32e-42 153
MS.gene045661.t1 MTR_4g124740 32.344 337 181 12 121 442 35 339 3.55e-42 152
MS.gene045661.t1 MTR_8g102380 33.648 318 168 12 86 389 40 328 1.74e-41 150
MS.gene045661.t1 MTR_5g012600 32.278 316 187 8 141 445 63 362 4.81e-41 150
MS.gene045661.t1 MTR_6g042660 34.286 280 159 8 141 415 37 296 1.57e-39 145
MS.gene045661.t1 MTR_5g088300 33.443 305 175 11 141 442 37 316 1.07e-38 143
MS.gene045661.t1 MTR_5g088280 31.613 310 182 10 137 442 35 318 1.12e-36 137
MS.gene045661.t1 MTR_4g051810 33.096 281 167 7 136 415 32 292 2.26e-36 136
MS.gene045661.t1 MTR_2g033970 31.250 320 187 9 134 444 53 348 1.07e-35 135
MS.gene045661.t1 MTR_6g042690 34.495 287 154 11 141 415 39 303 1.13e-35 135
MS.gene045661.t1 MTR_1g033050 33.099 284 163 9 143 415 17 284 2.10e-35 134
MS.gene045661.t1 MTR_5g088290 33.935 277 161 7 141 415 48 304 6.79e-35 133
MS.gene045661.t1 MTR_4g074290 29.377 337 205 13 122 442 42 361 1.17e-33 130
MS.gene045661.t1 MTR_2g073390 29.167 336 207 10 122 442 42 361 5.03e-33 128
MS.gene045661.t1 MTR_8g036225 32.168 286 163 8 141 415 33 298 1.46e-32 126
MS.gene045661.t1 MTR_0009s0350 29.412 340 201 14 122 442 42 361 2.37e-31 124
MS.gene045661.t1 MTR_0009s0340 29.412 340 201 14 122 442 42 361 2.37e-31 124
MS.gene045661.t1 MTR_2g436600 30.154 325 175 12 134 444 52 338 1.37e-29 118
MS.gene045661.t1 MTR_6g025590 35.196 179 107 3 143 313 51 228 3.90e-27 109
MS.gene045661.t1 MTR_4g130790 32.727 165 97 5 170 325 53 212 5.78e-17 80.9
MS.gene045661.t1 MTR_0408s0030 38.776 98 50 2 318 415 36 123 6.33e-15 72.4
MS.gene045661.t1 MTR_6g024200 32.203 177 91 6 270 442 125 276 6.52e-14 72.4
MS.gene045661.t1 MTR_2g436070 28.986 207 125 8 22 213 122 321 7.15e-14 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene045661.t1 AT5G51490 60.307 456 169 6 4 453 87 536 0.0 565
MS.gene045661.t1 AT3G47400 61.283 452 166 4 9 453 145 594 0.0 564
MS.gene045661.t1 AT5G51500 61.937 444 162 5 14 453 100 540 0.0 563
MS.gene045661.t1 AT2G45220 53.288 441 184 11 14 453 92 511 1.53e-152 443
MS.gene045661.t1 AT1G23200 46.091 486 212 10 1 453 86 554 2.16e-136 404
MS.gene045661.t1 AT3G60730 45.572 463 208 10 21 453 71 519 1.56e-132 392
MS.gene045661.t1 AT1G11580 48.655 409 198 6 50 453 156 557 1.19e-127 381
MS.gene045661.t1 AT1G11580 48.655 409 198 6 50 453 177 578 3.92e-127 380
MS.gene045661.t1 AT4G00190 45.786 439 211 9 16 453 62 474 6.83e-127 376
MS.gene045661.t1 AT3G14310 44.864 477 226 9 11 453 119 592 4.23e-125 376
MS.gene045661.t1 AT5G53370 46.341 451 226 8 16 453 138 585 4.94e-125 375
MS.gene045661.t1 AT3G49220 45.916 453 227 8 14 453 149 596 1.25e-124 375
MS.gene045661.t1 AT1G53830 45.860 471 222 10 11 453 122 587 1.79e-124 374
MS.gene045661.t1 AT5G53370 46.341 451 226 8 16 453 174 621 2.60e-124 375
MS.gene045661.t1 AT4G02320 46.050 443 225 8 21 453 80 518 2.62e-124 371
MS.gene045661.t1 AT3G49220 45.916 453 227 8 14 453 194 641 5.75e-124 374
MS.gene045661.t1 AT3G43270 44.519 447 230 7 21 453 83 525 1.02e-121 365
MS.gene045661.t1 AT3G59010 46.501 443 209 10 21 453 104 528 3.20e-121 363
MS.gene045661.t1 AT4G02300 42.291 454 244 8 11 453 86 532 1.14e-118 357
MS.gene045661.t1 AT4G33220 43.860 456 230 7 21 453 71 523 4.87e-118 355
MS.gene045661.t1 AT4G02330 41.598 488 241 9 8 453 86 571 4.32e-117 355
MS.gene045661.t1 AT4G33220 51.585 347 165 3 107 453 59 402 4.73e-117 348
MS.gene045661.t1 AT2G43050 45.227 440 213 9 20 453 99 516 2.04e-116 351
MS.gene045661.t1 AT3G05620 43.939 462 223 12 11 453 97 541 2.20e-116 352
MS.gene045661.t1 AT5G04960 45.023 442 217 12 22 451 135 562 5.32e-116 352
MS.gene045661.t1 AT5G27870 40.406 443 248 8 22 453 125 562 4.81e-112 346
MS.gene045661.t1 AT3G05610 40.674 445 246 8 22 453 126 565 7.77e-112 344
MS.gene045661.t1 AT1G02810 41.718 489 225 11 20 453 94 577 3.58e-110 337
MS.gene045661.t1 AT5G20860 40.879 455 248 9 7 453 97 538 5.97e-110 335
MS.gene045661.t1 AT3G10710 45.023 442 217 12 22 453 136 561 5.10e-109 333
MS.gene045661.t1 AT2G47550 43.290 462 230 9 20 453 101 558 7.87e-109 333
MS.gene045661.t1 AT3G10720 52.484 322 149 3 134 453 296 615 3.36e-107 330
MS.gene045661.t1 AT5G49180 42.029 414 222 8 50 449 157 566 2.64e-106 327
MS.gene045661.t1 AT2G26450 41.176 408 230 7 50 453 210 611 5.57e-105 325
MS.gene045661.t1 AT2G26440 41.037 463 246 8 8 453 95 547 7.60e-104 320
MS.gene045661.t1 AT4G33230 41.247 417 226 7 50 453 196 606 1.01e-103 321
MS.gene045661.t1 AT3G27980 43.921 403 214 8 52 453 106 497 1.35e-102 315
MS.gene045661.t1 AT3G06830 39.762 420 231 10 50 451 151 566 1.36e-102 317
MS.gene045661.t1 AT1G11590 43.735 407 217 7 50 453 127 524 6.16e-102 314
MS.gene045661.t1 AT1G53840 40.733 464 231 10 13 447 133 581 8.27e-102 316
MS.gene045661.t1 AT5G04970 49.068 322 160 3 134 453 301 620 8.33e-102 317
MS.gene045661.t1 AT4G03930 43.210 405 218 8 52 453 129 524 7.62e-101 311
MS.gene045661.t1 AT3G10720 56.322 261 112 2 193 453 1 259 3.56e-100 300
MS.gene045661.t1 AT3G14300 40.177 453 242 11 16 448 523 966 8.21e-93 301
MS.gene045661.t1 AT4G15980 46.087 345 174 8 114 451 361 700 1.99e-88 284
MS.gene045661.t1 AT4G15980 46.087 345 174 8 114 451 371 710 3.57e-88 283
MS.gene045661.t1 AT5G20860 37.530 421 215 7 7 419 97 477 8.95e-87 275
MS.gene045661.t1 AT5G09760 37.500 472 240 15 13 441 87 546 1.07e-83 268
MS.gene045661.t1 AT5G09760 37.500 472 240 15 13 441 113 572 2.83e-83 267
MS.gene045661.t1 AT2G47040 41.620 358 190 8 109 453 244 595 3.16e-81 262
MS.gene045661.t1 AT1G11370 49.393 247 124 1 138 384 43 288 8.62e-81 252
MS.gene045661.t1 AT2G47030 40.111 359 197 6 109 453 234 588 3.50e-79 257
MS.gene045661.t1 AT5G64640 39.243 423 222 13 50 446 189 602 2.09e-77 253
MS.gene045661.t1 AT3G62170 37.875 367 202 6 109 453 226 588 1.17e-72 239
MS.gene045661.t1 AT2G36700 34.810 316 184 7 133 442 34 333 6.92e-52 178
MS.gene045661.t1 AT3G29090 36.396 283 157 6 143 415 9 278 2.80e-51 176
MS.gene045661.t1 AT5G19730 33.233 331 189 9 123 442 72 381 1.31e-50 176
MS.gene045661.t1 AT2G36710 32.288 319 193 8 138 447 86 390 1.37e-47 169
MS.gene045661.t1 AT2G21610 33.993 303 178 7 141 442 51 332 4.57e-47 166
MS.gene045661.t1 AT2G21610 33.993 303 178 7 141 442 56 337 7.56e-47 166
MS.gene045661.t1 AT1G05310 33.754 317 180 8 139 442 89 388 1.11e-45 163
MS.gene045661.t1 AT5G47500 31.402 328 196 8 126 442 48 357 3.10e-41 150
MS.gene045661.t1 AT3G17060 32.797 311 182 9 141 443 46 337 1.72e-40 148
MS.gene045661.t1 AT5G07430 31.454 337 200 10 120 442 36 355 3.23e-40 148
MS.gene045661.t1 AT3G24130 33.667 300 163 9 132 415 25 304 4.22e-38 141
MS.gene045661.t1 AT5G55590 32.410 361 210 12 96 442 38 378 8.49e-38 142
MS.gene045661.t1 AT1G44980 42.105 190 106 3 120 306 54 242 1.54e-37 137
MS.gene045661.t1 AT5G55590 33.882 304 171 10 96 385 38 325 2.62e-36 136
MS.gene045661.t1 AT5G61680 30.794 315 188 10 135 442 67 358 1.75e-34 132
MS.gene045661.t1 AT5G26810 31.973 294 162 9 141 415 34 308 2.75e-33 128
MS.gene045661.t1 AT5G18990 32.069 290 165 8 140 415 28 299 3.27e-33 128
MS.gene045661.t1 AT5G07410 30.124 322 193 11 110 413 22 329 3.71e-33 129
MS.gene045661.t1 AT1G69940 29.938 324 195 11 110 415 22 331 6.26e-33 128
MS.gene045661.t1 AT5G07420 29.204 339 207 11 119 442 35 355 7.44e-32 125
MS.gene045661.t1 AT2G19150 29.483 329 196 9 134 449 34 339 3.11e-31 123
MS.gene045661.t1 AT2G19150 30.943 265 151 7 134 385 34 279 1.18e-28 114
MS.gene045661.t1 AT3G49220 32.735 223 133 7 14 223 194 412 1.65e-23 102
MS.gene045661.t1 AT2G47280 30.272 294 169 10 137 415 31 303 3.15e-23 100

Find 115 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAATTGGTACAATGAATTT+TGG 0.141708 4.3:-64164183 None:intergenic
ATGTTTGCTTGACCGTTTAA+AGG 0.191682 4.3:-64165067 None:intergenic
TTCTACCGTTGTGCCTTTAA+AGG 0.221697 4.3:+64164914 MS.gene045661:CDS
ATGTTTATAACTTTCATAAT+TGG 0.257834 4.3:-64164198 None:intergenic
CCGACAACGGGCTTGAAATC+TGG 0.267143 4.3:-64165166 None:intergenic
GGAACCTTCAAGGGTTATTA+AGG 0.287555 4.3:+64164353 MS.gene045661:CDS
GGTTCGAGAGAGGAAATTAT+TGG 0.287986 4.3:+64164332 MS.gene045661:CDS
GTACAAGAATGATGCAGTTT+TGG 0.293323 4.3:-64165039 None:intergenic
TACAGAGAATGTTACATCTA+TGG 0.298252 4.3:+64164977 MS.gene045661:CDS
GGCACCGTTGACTTCATCTT+TGG 0.305498 4.3:+64164998 MS.gene045661:CDS
CATCTTGTACTCTCTTAAAA+TGG 0.314188 4.3:-64164407 None:intergenic
CAATTCACAACTCTCAGATT+AGG 0.315294 4.3:+64165137 MS.gene045661:CDS
GGTGTCTTGGTAGCCTTTAA+AGG 0.325796 4.3:-64164927 None:intergenic
GCAATGGTTTATTCGTCAAT+TGG 0.333480 4.3:+64164235 MS.gene045661:CDS
AATTCACAACTCTCAGATTA+GGG 0.336514 4.3:+64165138 MS.gene045661:CDS
TTGTGTACTATAGGAATTGA+TGG 0.340716 4.3:+64164794 MS.gene045661:intron
ACTGTTGCTACCCTCCTTGC+TGG 0.366831 4.3:+64165430 MS.gene045661:CDS
TTGCCAGGGCCGGCCCAAAA+AGG 0.367122 4.3:-64165200 None:intergenic
CTATTTCTTGTTGATGATCC+AGG 0.370812 4.3:-64165352 None:intergenic
GTCCAATGGGGTGGTCTCCA+TGG 0.375821 4.3:+64165272 MS.gene045661:CDS
AACATCGAAACATGTCGAAA+TGG 0.379750 4.3:+64164141 MS.gene045661:CDS
CAATGGTTTATTCGTCAATT+GGG 0.384937 4.3:+64164236 MS.gene045661:CDS
TCCAATGGGGTGGTCTCCAT+GGG 0.393186 4.3:+64165273 MS.gene045661:CDS
ACATGTTTCGATGTTTGTAT+AGG 0.395429 4.3:-64164133 None:intergenic
ACATCGAAACATGTCGAAAT+GGG 0.395879 4.3:+64164142 MS.gene045661:CDS
GTGTTGAATTTCCCGACAAC+GGG 0.400235 4.3:-64165178 None:intergenic
TATGGAGAATACAAGAACTT+TGG 0.406134 4.3:+64165328 MS.gene045661:CDS
AAATGGGTCATCTCGACCTT+GGG 0.407983 4.3:-64165098 None:intergenic
GGAAATTATTGGAACCTTCA+AGG 0.413393 4.3:+64164343 MS.gene045661:CDS
GAAATCATTCATTGATAGCT+TGG 0.423252 4.3:+64165246 MS.gene045661:CDS
TGCGCCTTAATAACCCTTGA+AGG 0.430775 4.3:-64164357 None:intergenic
ATAATTTCGGACAAAATAGT+TGG 0.431028 4.3:+64163968 MS.gene045661:CDS
GATAGCTTGGTCAGTCCAAT+GGG 0.431876 4.3:+64165259 MS.gene045661:CDS
CATATCATTGTCGCGAAAGA+TGG 0.433386 4.3:+64164378 MS.gene045661:CDS
CTGAAGAAGGGTTTCCAAGA+TGG 0.434790 4.3:+64164304 MS.gene045661:CDS
AACTCTGGAGTATCTTTGCC+AGG 0.437542 4.3:-64165215 None:intergenic
TCTCGAACCGAAAACCATCT+TGG 0.449598 4.3:-64164318 None:intergenic
CATTGTCGCGAAAGATGGTT+CGG 0.450110 4.3:+64164383 MS.gene045661:CDS
CCAGATTTCAAGCCCGTTGT+CGG 0.455066 4.3:+64165166 MS.gene045661:CDS
TTAAACGTGATGGCCGTGTT+CGG 0.459371 4.3:+64164265 MS.gene045661:CDS
ACTGGTGTACCATTCACTTC+TGG 0.465852 4.3:+64165472 MS.gene045661:CDS
GAAATGGGTCATCTCGACCT+TGG 0.467978 4.3:-64165099 None:intergenic
CAGATTTCAAGCCCGTTGTC+GGG 0.471943 4.3:+64165167 MS.gene045661:CDS
ACCTGCAGTGGCAGAACTAT+AGG 0.476593 4.3:-64164607 None:intergenic
CTTGTACGAAGGCCTTTAAA+CGG 0.479605 4.3:+64165055 MS.gene045661:CDS
GAAATTATTGGAACCTTCAA+GGG 0.482132 4.3:+64164344 MS.gene045661:CDS
ATAGAGAGAATATTGTGGTT+GGG 0.482521 4.3:+64164496 MS.gene045661:CDS
CAAGATGCTATAAATGCTGC+TGG 0.484980 4.3:+64164423 MS.gene045661:CDS
AATATTATGTTGGTTGGTGA+TGG 0.489232 4.3:+64164531 MS.gene045661:CDS
TATAGAGAGAATATTGTGGT+TGG 0.491777 4.3:+64164495 MS.gene045661:CDS
TGATAGCTTGGTCAGTCCAA+TGG 0.494017 4.3:+64165258 MS.gene045661:CDS
CCCTCCTTGCTGGCACCACA+TGG 0.495961 4.3:+64165440 MS.gene045661:CDS
ACCAACGATGTAACTGAAGA+AGG 0.504546 4.3:+64164291 MS.gene045661:CDS
AGAAATAGAGTAAGATGGCC+TGG 0.507966 4.3:+64165367 MS.gene045661:CDS
TGGAGTATCTTTGCCAGGGC+CGG 0.508397 4.3:-64165210 None:intergenic
AGTAATAGAAATGTTCAAGC+TGG 0.512268 4.3:+64164576 MS.gene045661:CDS
GTGAATGGTGATGCTTGAGC+TGG 0.512449 4.3:-64165409 None:intergenic
AATGATAATATTATGTTGGT+TGG 0.513930 4.3:+64164525 MS.gene045661:CDS
TAATTTCGGACAAAATAGTT+GGG 0.519031 4.3:+64163969 MS.gene045661:CDS
CCCCATGGAGACCACCCCAT+TGG 0.520496 4.3:-64165274 None:intergenic
TGATTTCATGACCACAACTC+TGG 0.520918 4.3:-64165230 None:intergenic
GATGAATGATAATATTATGT+TGG 0.524956 4.3:+64164521 MS.gene045661:CDS
ACCTATAGTTCTGCCACTGC+AGG 0.525895 4.3:+64164606 MS.gene045661:CDS
TAAATGCTGCTGGGAGGAGA+AGG 0.527517 4.3:+64164433 MS.gene045661:CDS
AACATGTCGAAATGGGGCAT+TGG 0.527557 4.3:+64164149 MS.gene045661:CDS
CCCTTCTTCAGTTACATCGT+TGG 0.528030 4.3:-64164292 None:intergenic
CCATGTGGTGCCAGCAAGGA+GGG 0.530412 4.3:-64165440 None:intergenic
ACCACATGGTTACCTGCCAC+TGG 0.533118 4.3:+64165454 MS.gene045661:CDS
AGGAGGGTAGCAACAGTGAA+TGG 0.537056 4.3:-64165424 None:intergenic
ATGGGGTGGTCTCCATGGGG+AGG 0.545885 4.3:+64165277 MS.gene045661:CDS
AAGAATATCATTACCTGCAG+TGG 0.552651 4.3:-64164619 None:intergenic
AATTAAAGGCCAGAAGTGAA+TGG 0.553145 4.3:-64165481 None:intergenic
CAACAAGAAATAGAGTAAGA+TGG 0.559275 4.3:+64165362 MS.gene045661:CDS
CCAACGATGTAACTGAAGAA+GGG 0.560246 4.3:+64164292 MS.gene045661:CDS
CGTGAACCATGAGGGTGTCT+TGG 0.562307 4.3:-64164940 None:intergenic
TGGGAATTCGTTAAACGTGA+TGG 0.567493 4.3:+64164255 MS.gene045661:CDS
GGTGTTGAATTTCCCGACAA+CGG 0.567667 4.3:-64165179 None:intergenic
GATGACCCATTTCAAAACAC+TGG 0.574517 4.3:+64165109 MS.gene045661:CDS
GTGAATGGTACACCAGTGGC+AGG 0.575851 4.3:-64165466 None:intergenic
ATGACACAAATCATTAGCAA+TGG 0.576321 4.3:+64164219 MS.gene045661:CDS
ATTGTCGCGAAAGATGGTTC+GGG 0.578990 4.3:+64164384 MS.gene045661:CDS
TGCACAAACGTGGCTAAGCA+CGG 0.583362 4.3:+64164110 MS.gene045661:CDS
TGAATGGTGATGCTTGAGCT+GGG 0.585698 4.3:-64165408 None:intergenic
CATCGTTGGTGTACCGAACA+CGG 0.586715 4.3:-64164278 None:intergenic
GCAAACATGATCACGGCCCA+AGG 0.588675 4.3:+64165082 MS.gene045661:CDS
TTTGTTATACGTGTGAAGAG+AGG 0.590375 4.3:+64164468 MS.gene045661:CDS
ACCACGTCGTGGTCAAGCAG+TGG 0.603947 4.3:+64164862 MS.gene045661:CDS
ACTGCATCATTCTTGTACGA+AGG 0.614280 4.3:+64165044 MS.gene045661:CDS
GCTTGGTCAGTCCAATGGGG+TGG 0.614700 4.3:+64165263 MS.gene045661:CDS
TGTTTATAGAGAGAATATTG+TGG 0.617943 4.3:+64164491 MS.gene045661:CDS
CCAATGGGGTGGTCTCCATG+GGG 0.626856 4.3:+64165274 MS.gene045661:CDS
CATCGAAACATGTCGAAATG+GGG 0.628859 4.3:+64164143 MS.gene045661:CDS
GTAACCATGTGGTGCCAGCA+AGG 0.628912 4.3:-64165444 None:intergenic
GCAAAGATACTCCAGAGTTG+TGG 0.635768 4.3:+64165219 MS.gene045661:CDS
GAATACAAGAACTTTGGACC+TGG 0.637921 4.3:+64165334 MS.gene045661:CDS
CGGTCAAGCAAACATGATCA+CGG 0.638793 4.3:+64165075 MS.gene045661:CDS
AAGATGCTATAAATGCTGCT+GGG 0.644279 4.3:+64164424 MS.gene045661:CDS
GTTGCCAAAGATGAAGTCAA+CGG 0.646875 4.3:-64165002 None:intergenic
AGGCTACCAAGACACCCTCA+TGG 0.652925 4.3:+64164934 MS.gene045661:CDS
AACGGGCTTGAAATCTGGAG+CGG 0.657191 4.3:-64165161 None:intergenic
CATCATTCGATGCACAAACG+TGG 0.658365 4.3:+64164100 MS.gene045661:CDS
CACAACGGTAGAAAACAGAT+AGG 0.659204 4.3:-64164904 None:intergenic
ACTCTGGAGTATCTTTGCCA+GGG 0.660708 4.3:-64165214 None:intergenic
ACCATGTGGTGCCAGCAAGG+AGG 0.665765 4.3:-64165441 None:intergenic
GCAAAATTAGTACCTCCCCA+TGG 0.675248 4.3:-64165289 None:intergenic
AACACGGCTGGACCACGTCG+TGG 0.685767 4.3:+64164851 MS.gene045661:CDS
ATGCTATAAATGCTGCTGGG+AGG 0.686085 4.3:+64164427 MS.gene045661:CDS
AGAAGTGAATGGTACACCAG+TGG 0.693790 4.3:-64165470 None:intergenic
GCCACTGCTTGACCACGACG+TGG 0.699801 4.3:-64164863 None:intergenic
GACGTTGTGCGTGAACCATG+AGG 0.708175 4.3:-64164949 None:intergenic
GTAGAAAACAGATAGGTCAG+AGG 0.708320 4.3:-64164897 None:intergenic
ACGTTGTGCGTGAACCATGA+GGG 0.708924 4.3:-64164948 None:intergenic
GGTAGCCTTTAAAGGCACAA+CGG 0.723796 4.3:-64164919 None:intergenic
ACCAGTGGCAGGTAACCATG+TGG 0.747350 4.3:-64165455 None:intergenic
ATAGCTTGGTCAGTCCAATG+GGG 0.769302 4.3:+64165260 MS.gene045661:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGTTTATAACTTTCATAAT+TGG - chr4.3:64164201-64164220 None:intergenic 15.0%
!!! CAACTAAATTTTTTTTCTTT+AGG + chr4.3:64164669-64164688 MS.gene045661:intron 15.0%
!!! TAATTTTTTTTGTGTACTAT+AGG + chr4.3:64164785-64164804 MS.gene045661:intron 15.0%
!! AATGATAATATTATGTTGGT+TGG + chr4.3:64164525-64164544 MS.gene045661:CDS 20.0%
!! ATATATAACAATAAGGAAAG+AGG - chr4.3:64164719-64164738 None:intergenic 20.0%
!! GATGAATGATAATATTATGT+TGG + chr4.3:64164521-64164540 MS.gene045661:CDS 20.0%
!! GTTAGAGATATATAACAATA+AGG - chr4.3:64164726-64164745 None:intergenic 20.0%
!!! ATAATTGGTACAATGAATTT+TGG - chr4.3:64164186-64164205 None:intergenic 20.0%
! ATAATTTCGGACAAAATAGT+TGG + chr4.3:64163968-64163987 MS.gene045661:CDS 25.0%
! TAATTTCGGACAAAATAGTT+GGG + chr4.3:64163969-64163988 MS.gene045661:CDS 25.0%
! TGTTTATAGAGAGAATATTG+TGG + chr4.3:64164491-64164510 MS.gene045661:CDS 25.0%
!!! AAATTCCAGTGTTTTGAAAT+GGG - chr4.3:64165117-64165136 None:intergenic 25.0%
!!! ATGAACATGATTTTTTCTTG+TGG - chr4.3:64164083-64164102 None:intergenic 25.0%
!!! TGACACTGATTTTTTCTTTT+GGG - chr4.3:64163998-64164017 None:intergenic 25.0%
!!! TGACATTACTTTTGAAAACA+CGG + chr4.3:64164835-64164854 MS.gene045661:CDS 25.0%
!!! TTCTTAGCTATTTTTCCTTA+TGG + chr4.3:64164637-64164656 MS.gene045661:intron 25.0%
AATTCACAACTCTCAGATTA+GGG + chr4.3:64165138-64165157 MS.gene045661:CDS 30.0%
ACATGTTTCGATGTTTGTAT+AGG - chr4.3:64164136-64164155 None:intergenic 30.0%
AGTAATAGAAATGTTCAAGC+TGG + chr4.3:64164576-64164595 MS.gene045661:CDS 30.0%
ATAGAGAGAATATTGTGGTT+GGG + chr4.3:64164496-64164515 MS.gene045661:CDS 30.0%
ATGACACAAATCATTAGCAA+TGG + chr4.3:64164219-64164238 MS.gene045661:CDS 30.0%
CAACAAGAAATAGAGTAAGA+TGG + chr4.3:64165362-64165381 MS.gene045661:CDS 30.0%
CAATGGTTTATTCGTCAATT+GGG + chr4.3:64164236-64164255 MS.gene045661:CDS 30.0%
CATCTTGTACTCTCTTAAAA+TGG - chr4.3:64164410-64164429 None:intergenic 30.0%
GAAATTATTGGAACCTTCAA+GGG + chr4.3:64164344-64164363 MS.gene045661:CDS 30.0%
TACAGAGAATGTTACATCTA+TGG + chr4.3:64164977-64164996 MS.gene045661:CDS 30.0%
TAGTTGAATTTGACTCCATA+AGG - chr4.3:64164655-64164674 None:intergenic 30.0%
TATAGAGAGAATATTGTGGT+TGG + chr4.3:64164495-64164514 MS.gene045661:CDS 30.0%
TATGGAGAATACAAGAACTT+TGG + chr4.3:64165328-64165347 MS.gene045661:CDS 30.0%
TTGTGTACTATAGGAATTGA+TGG + chr4.3:64164794-64164813 MS.gene045661:intron 30.0%
! CTTTTCATAACATGATAACC+AGG - chr4.3:64165388-64165407 None:intergenic 30.0%
! GAAATCATTCATTGATAGCT+TGG + chr4.3:64165246-64165265 MS.gene045661:CDS 30.0%
!! AATATTATGTTGGTTGGTGA+TGG + chr4.3:64164531-64164550 MS.gene045661:CDS 30.0%
!!! AACATGATTTTTTCTTGTGG+AGG - chr4.3:64164080-64164099 None:intergenic 30.0%
!!! GAAATTCCAGTGTTTTGAAA+TGG - chr4.3:64165118-64165137 None:intergenic 30.0%
!!! GTGACACTGATTTTTTCTTT+TGG - chr4.3:64163999-64164018 None:intergenic 30.0%
AACATCGAAACATGTCGAAA+TGG + chr4.3:64164141-64164160 MS.gene045661:CDS 35.0%
AAGAATATCATTACCTGCAG+TGG - chr4.3:64164622-64164641 None:intergenic 35.0%
AAGATGCTATAAATGCTGCT+GGG + chr4.3:64164424-64164443 MS.gene045661:CDS 35.0%
ACATCGAAACATGTCGAAAT+GGG + chr4.3:64164142-64164161 MS.gene045661:CDS 35.0%
ATGTTTGCTTGACCGTTTAA+AGG - chr4.3:64165070-64165089 None:intergenic 35.0%
CAATTCACAACTCTCAGATT+AGG + chr4.3:64165137-64165156 MS.gene045661:CDS 35.0%
CTAAAAATGTACGGTTGAGA+TGG - chr4.3:64164056-64164075 None:intergenic 35.0%
GCAATGGTTTATTCGTCAAT+TGG + chr4.3:64164235-64164254 MS.gene045661:CDS 35.0%
GGAAATTATTGGAACCTTCA+AGG + chr4.3:64164343-64164362 MS.gene045661:CDS 35.0%
TGTGGAGGTCTAAAAATGTA+CGG - chr4.3:64164065-64164084 None:intergenic 35.0%
! GGGAAATTCAACACCTTTTT+GGG + chr4.3:64165187-64165206 MS.gene045661:CDS 35.0%
! GTACAAGAATGATGCAGTTT+TGG - chr4.3:64165042-64165061 None:intergenic 35.0%
! TTTGTTATACGTGTGAAGAG+AGG + chr4.3:64164468-64164487 MS.gene045661:CDS 35.0%
!! ATTACTTTTGAAAACACGGC+TGG + chr4.3:64164839-64164858 MS.gene045661:CDS 35.0%
!! CTATTTCTTGTTGATGATCC+AGG - chr4.3:64165355-64165374 None:intergenic 35.0%
!! GCTCAAAGCACTCTATTTTA+TGG + chr4.3:64165310-64165329 MS.gene045661:CDS 35.0%
!!! GATGCAGTTTTGGAAAACTA+CGG - chr4.3:64165032-64165051 None:intergenic 35.0%
ACCAACGATGTAACTGAAGA+AGG + chr4.3:64164291-64164310 MS.gene045661:CDS 40.0%
ACTGCATCATTCTTGTACGA+AGG + chr4.3:64165044-64165063 MS.gene045661:CDS 40.0%
AGAAATAGAGTAAGATGGCC+TGG + chr4.3:64165367-64165386 MS.gene045661:CDS 40.0%
CAAGATGCTATAAATGCTGC+TGG + chr4.3:64164423-64164442 MS.gene045661:CDS 40.0%
CACAACGGTAGAAAACAGAT+AGG - chr4.3:64164907-64164926 None:intergenic 40.0%
CATATCATTGTCGCGAAAGA+TGG + chr4.3:64164378-64164397 MS.gene045661:CDS 40.0%
CATCGAAACATGTCGAAATG+GGG + chr4.3:64164143-64164162 MS.gene045661:CDS 40.0%
CCAACGATGTAACTGAAGAA+GGG + chr4.3:64164292-64164311 MS.gene045661:CDS 40.0%
CTTGTACGAAGGCCTTTAAA+CGG + chr4.3:64165055-64165074 MS.gene045661:CDS 40.0%
GAATACAAGAACTTTGGACC+TGG + chr4.3:64165334-64165353 MS.gene045661:CDS 40.0%
GATGACCCATTTCAAAACAC+TGG + chr4.3:64165109-64165128 MS.gene045661:CDS 40.0%
GGAACCTTCAAGGGTTATTA+AGG + chr4.3:64164353-64164372 MS.gene045661:CDS 40.0%
GGTTCGAGAGAGGAAATTAT+TGG + chr4.3:64164332-64164351 MS.gene045661:CDS 40.0%
GTAGAAAACAGATAGGTCAG+AGG - chr4.3:64164900-64164919 None:intergenic 40.0%
GTTGCCAAAGATGAAGTCAA+CGG - chr4.3:64165005-64165024 None:intergenic 40.0%
TGATTTCATGACCACAACTC+TGG - chr4.3:64165233-64165252 None:intergenic 40.0%
TGGGAATTCGTTAAACGTGA+TGG + chr4.3:64164255-64164274 MS.gene045661:CDS 40.0%
TTCTACCGTTGTGCCTTTAA+AGG + chr4.3:64164914-64164933 MS.gene045661:CDS 40.0%
! AGGGTTTCCAAGATGGTTTT+CGG + chr4.3:64164311-64164330 MS.gene045661:CDS 40.0%
! CGGGAAATTCAACACCTTTT+TGG + chr4.3:64165186-64165205 MS.gene045661:CDS 40.0%
!! AATTCAACACCTTTTTGGGC+CGG + chr4.3:64165191-64165210 MS.gene045661:CDS 40.0%
AAATGGGTCATCTCGACCTT+GGG - chr4.3:64165101-64165120 None:intergenic 45.0%
AACATGTCGAAATGGGGCAT+TGG + chr4.3:64164149-64164168 MS.gene045661:CDS 45.0%
ACTGGTGTACCATTCACTTC+TGG + chr4.3:64165472-64165491 MS.gene045661:CDS 45.0%
AGAAGTGAATGGTACACCAG+TGG - chr4.3:64165473-64165492 None:intergenic 45.0%
ATAGCTTGGTCAGTCCAATG+GGG + chr4.3:64165260-64165279 MS.gene045661:CDS 45.0%
ATGCTATAAATGCTGCTGGG+AGG + chr4.3:64164427-64164446 MS.gene045661:CDS 45.0%
ATTGTCGCGAAAGATGGTTC+GGG + chr4.3:64164384-64164403 MS.gene045661:CDS 45.0%
CATCATTCGATGCACAAACG+TGG + chr4.3:64164100-64164119 MS.gene045661:CDS 45.0%
CATTGTCGCGAAAGATGGTT+CGG + chr4.3:64164383-64164402 MS.gene045661:CDS 45.0%
CCCTTCTTCAGTTACATCGT+TGG - chr4.3:64164295-64164314 None:intergenic 45.0%
CGGTCAAGCAAACATGATCA+CGG + chr4.3:64165075-64165094 MS.gene045661:CDS 45.0%
CTGAAGAAGGGTTTCCAAGA+TGG + chr4.3:64164304-64164323 MS.gene045661:CDS 45.0%
GATAGCTTGGTCAGTCCAAT+GGG + chr4.3:64165259-64165278 MS.gene045661:CDS 45.0%
GCAAAATTAGTACCTCCCCA+TGG - chr4.3:64165292-64165311 None:intergenic 45.0%
GCAAAGATACTCCAGAGTTG+TGG + chr4.3:64165219-64165238 MS.gene045661:CDS 45.0%
GTGTTGAATTTCCCGACAAC+GGG - chr4.3:64165181-64165200 None:intergenic 45.0%
TCTCGAACCGAAAACCATCT+TGG - chr4.3:64164321-64164340 None:intergenic 45.0%
TGATAGCTTGGTCAGTCCAA+TGG + chr4.3:64165258-64165277 MS.gene045661:CDS 45.0%
TGCGCCTTAATAACCCTTGA+AGG - chr4.3:64164360-64164379 None:intergenic 45.0%
TTAAACGTGATGGCCGTGTT+CGG + chr4.3:64164265-64164284 MS.gene045661:CDS 45.0%
! GGTAGCCTTTAAAGGCACAA+CGG - chr4.3:64164922-64164941 None:intergenic 45.0%
! GGTGTCTTGGTAGCCTTTAA+AGG - chr4.3:64164930-64164949 None:intergenic 45.0%
! GGTGTTGAATTTCCCGACAA+CGG - chr4.3:64165182-64165201 None:intergenic 45.0%
! TGAATGGTGATGCTTGAGCT+GGG - chr4.3:64165411-64165430 None:intergenic 45.0%
!! AACTCTGGAGTATCTTTGCC+AGG - chr4.3:64165218-64165237 None:intergenic 45.0%
!! ACTCTGGAGTATCTTTGCCA+GGG - chr4.3:64165217-64165236 None:intergenic 45.0%
ACCTGCAGTGGCAGAACTAT+AGG - chr4.3:64164610-64164629 None:intergenic 50.0%
ACGTTGTGCGTGAACCATGA+GGG - chr4.3:64164951-64164970 None:intergenic 50.0%
AGGAGGGTAGCAACAGTGAA+TGG - chr4.3:64165427-64165446 None:intergenic 50.0%
CAGATTTCAAGCCCGTTGTC+GGG + chr4.3:64165167-64165186 MS.gene045661:CDS 50.0%
CATCGTTGGTGTACCGAACA+CGG - chr4.3:64164281-64164300 None:intergenic 50.0%
CCAGATTTCAAGCCCGTTGT+CGG + chr4.3:64165166-64165185 MS.gene045661:CDS 50.0%
GAAATGGGTCATCTCGACCT+TGG - chr4.3:64165102-64165121 None:intergenic 50.0%
TAAATGCTGCTGGGAGGAGA+AGG + chr4.3:64164433-64164452 MS.gene045661:CDS 50.0%
TGCACAAACGTGGCTAAGCA+CGG + chr4.3:64164110-64164129 MS.gene045661:CDS 50.0%
! AACGGGCTTGAAATCTGGAG+CGG - chr4.3:64165164-64165183 None:intergenic 50.0%
! ACCTATAGTTCTGCCACTGC+AGG + chr4.3:64164606-64164625 MS.gene045661:CDS 50.0%
! GTGAATGGTGATGCTTGAGC+TGG - chr4.3:64165412-64165431 None:intergenic 50.0%
!! GATGGTTTTCGGTTCGAGAG+AGG + chr4.3:64164322-64164341 MS.gene045661:CDS 50.0%
!! GGCACCGTTGACTTCATCTT+TGG + chr4.3:64164998-64165017 MS.gene045661:CDS 50.0%
ACCACATGGTTACCTGCCAC+TGG + chr4.3:64165454-64165473 MS.gene045661:CDS 55.0%
ACCAGTGGCAGGTAACCATG+TGG - chr4.3:64165458-64165477 None:intergenic 55.0%
ACTGTTGCTACCCTCCTTGC+TGG + chr4.3:64165430-64165449 MS.gene045661:CDS 55.0%
AGGCTACCAAGACACCCTCA+TGG + chr4.3:64164934-64164953 MS.gene045661:CDS 55.0%
GACGTTGTGCGTGAACCATG+AGG - chr4.3:64164952-64164971 None:intergenic 55.0%
GCAAACATGATCACGGCCCA+AGG + chr4.3:64165082-64165101 MS.gene045661:CDS 55.0%
GTGAATGGTACACCAGTGGC+AGG - chr4.3:64165469-64165488 None:intergenic 55.0%
TCCAATGGGGTGGTCTCCAT+GGG + chr4.3:64165273-64165292 MS.gene045661:CDS 55.0%
! CCGACAACGGGCTTGAAATC+TGG - chr4.3:64165169-64165188 None:intergenic 55.0%
! GTAACCATGTGGTGCCAGCA+AGG - chr4.3:64165447-64165466 None:intergenic 55.0%
! TGGAGTATCTTTGCCAGGGC+CGG - chr4.3:64165213-64165232 None:intergenic 55.0%
!! CGTGAACCATGAGGGTGTCT+TGG - chr4.3:64164943-64164962 None:intergenic 55.0%
ACCACGTCGTGGTCAAGCAG+TGG + chr4.3:64164862-64164881 MS.gene045661:CDS 60.0%
CCAATGGGGTGGTCTCCATG+GGG + chr4.3:64165274-64165293 MS.gene045661:CDS 60.0%
GCTTGGTCAGTCCAATGGGG+TGG + chr4.3:64165263-64165282 MS.gene045661:CDS 60.0%
GTCCAATGGGGTGGTCTCCA+TGG + chr4.3:64165272-64165291 MS.gene045661:CDS 60.0%
! ACCATGTGGTGCCAGCAAGG+AGG - chr4.3:64165444-64165463 None:intergenic 60.0%
! CCATGTGGTGCCAGCAAGGA+GGG - chr4.3:64165443-64165462 None:intergenic 60.0%
AACACGGCTGGACCACGTCG+TGG + chr4.3:64164851-64164870 MS.gene045661:CDS 65.0%
ATGGGGTGGTCTCCATGGGG+AGG + chr4.3:64165277-64165296 MS.gene045661:CDS 65.0%
CCCCATGGAGACCACCCCAT+TGG - chr4.3:64165277-64165296 None:intergenic 65.0%
CCCTCCTTGCTGGCACCACA+TGG + chr4.3:64165440-64165459 MS.gene045661:CDS 65.0%
GCCACTGCTTGACCACGACG+TGG - chr4.3:64164866-64164885 None:intergenic 65.0%
TTGCCAGGGCCGGCCCAAAA+AGG - chr4.3:64165203-64165222 None:intergenic 65.0%
!! ACACCTTTTTGGGCCGGCCC+TGG + chr4.3:64165197-64165216 MS.gene045661:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 64163961 64165501 64163961 ID=MS.gene045661
chr4.3 mRNA 64163961 64165501 64163961 ID=MS.gene045661.t1;Parent=MS.gene045661
chr4.3 exon 64163961 64164627 64163961 ID=MS.gene045661.t1.exon1;Parent=MS.gene045661.t1
chr4.3 CDS 64163961 64164627 64163961 ID=cds.MS.gene045661.t1;Parent=MS.gene045661.t1
chr4.3 exon 64164807 64165501 64164807 ID=MS.gene045661.t1.exon2;Parent=MS.gene045661.t1
chr4.3 CDS 64164807 64165501 64164807 ID=cds.MS.gene045661.t1;Parent=MS.gene045661.t1
Gene Sequence

>MS.gene045661

ATGGCGCATAATTTCGGACAAAATAGTTGGGTCACCCAAAAGAAAAAATCAGTGTCACGTGATTGTTTAAAACTCTTCGACAACACAGTTTTCCATCTCAACCGTACATTTTTAGACCTCCACAAGAAAAAATCATGTTCATCATTCGATGCACAAACGTGGCTAAGCACGGCCTATACAAACATCGAAACATGTCGAAATGGGGCATTGGAATTGAACATTCCAAAATTCATTGTACCAATTATGAAAGTTATAAACATGACACAAATCATTAGCAATGGTTTATTCGTCAATTGGGAATTCGTTAAACGTGATGGCCGTGTTCGGTACACCAACGATGTAACTGAAGAAGGGTTTCCAAGATGGTTTTCGGTTCGAGAGAGGAAATTATTGGAACCTTCAAGGGTTATTAAGGCGCATATCATTGTCGCGAAAGATGGTTCGGGCCATTTTAAGAGAGTACAAGATGCTATAAATGCTGCTGGGAGGAGAAGGTTTAAAACAAGATTTGTTATACGTGTGAAGAGAGGTGTTTATAGAGAGAATATTGTGGTTGGGAAGATGAATGATAATATTATGTTGGTTGGTGATGGAAGTAGATATACAGTGATAACAAGTAATAGAAATGTTCAAGCTGGTTTTACTACCTATAGTTCTGCCACTGCAGGTAATGATATTCTTAGCTATTTTTCCTTATGGAGTCAAATTCAACTAAATTTTTTTTCTTTAGGAATATGAATTCTATTCATTCTTCTCCTCTTTCCTTATTGTTATATATCTCTAACTATTATTATCATAACAAATTCAAATCAACACATTCTAAATAATTTTTTTTGTGTACTATAGGAATTGATGGTCTTCACTTCATTGCACGTGACATTACTTTTGAAAACACGGCTGGACCACGTCGTGGTCAAGCAGTGGCGCTTCGATCAGCCTCTGACCTATCTGTTTTCTACCGTTGTGCCTTTAAAGGCTACCAAGACACCCTCATGGTTCACGCACAACGTCAATTTTACAGAGAATGTTACATCTATGGCACCGTTGACTTCATCTTTGGCAACGCAGCCGTAGTTTTCCAAAACTGCATCATTCTTGTACGAAGGCCTTTAAACGGTCAAGCAAACATGATCACGGCCCAAGGTCGAGATGACCCATTTCAAAACACTGGAATTTCAATTCACAACTCTCAGATTAGGGCCGCTCCAGATTTCAAGCCCGTTGTCGGGAAATTCAACACCTTTTTGGGCCGGCCCTGGCAAAGATACTCCAGAGTTGTGGTCATGAAATCATTCATTGATAGCTTGGTCAGTCCAATGGGGTGGTCTCCATGGGGAGGTACTAATTTTGCTCAAAGCACTCTATTTTATGGAGAATACAAGAACTTTGGACCTGGATCATCAACAAGAAATAGAGTAAGATGGCCTGGTTATCATGTTATGAAAAGCCCAGCTCAAGCATCACCATTCACTGTTGCTACCCTCCTTGCTGGCACCACATGGTTACCTGCCACTGGTGTACCATTCACTTCTGGCCTTTAA
Protein sequence

>MS.gene045661.t1

MAHNFGQNSWVTQKKKSVSRDCLKLFDNTVFHLNRTFLDLHKKKSCSSFDAQTWLSTAYTNIETCRNGALELNIPKFIVPIMKVINMTQIISNGLFVNWEFVKRDGRVRYTNDVTEEGFPRWFSVRERKLLEPSRVIKAHIIVAKDGSGHFKRVQDAINAAGRRRFKTRFVIRVKRGVYRENIVVGKMNDNIMLVGDGSRYTVITSNRNVQAGFTTYSSATAGIDGLHFIARDITFENTAGPRRGQAVALRSASDLSVFYRCAFKGYQDTLMVHAQRQFYRECYIYGTVDFIFGNAAVVFQNCIILVRRPLNGQANMITAQGRDDPFQNTGISIHNSQIRAAPDFKPVVGKFNTFLGRPWQRYSRVVVMKSFIDSLVSPMGWSPWGGTNFAQSTLFYGEYKNFGPGSSTRNRVRWPGYHVMKSPAQASPFTVATLLAGTTWLPATGVPFTSGL