AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene047236

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene047236.t1 MTR_2g005570 98.565 418 6 0 1 418 1 418 0.0 854
MS.gene047236.t1 MTR_2g005570 98.325 418 7 0 1 418 1 418 0.0 852
MS.gene047236.t1 MTR_3g058940 89.021 419 42 3 3 418 2 419 0.0 769
MS.gene047236.t1 MTR_4g134770 83.055 419 67 3 3 418 2 419 0.0 678
MS.gene047236.t1 MTR_4g134770 84.896 384 55 2 38 418 1 384 0.0 637
MS.gene047236.t1 MTR_3g064700 30.233 172 107 6 42 203 40 208 3.29e-13 68.9
MS.gene047236.t1 MTR_1g088850 27.014 211 125 5 13 205 11 210 8.45e-12 64.7
MS.gene047236.t1 MTR_3g450790 25.714 175 113 4 42 204 39 208 1.08e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene047236.t1 AT1G09640 76.555 418 91 4 3 418 2 414 0.0 641
MS.gene047236.t1 AT1G57720 74.341 417 101 5 3 418 2 413 0.0 627
MS.gene047236.t1 AT1G57720 74.341 417 101 5 3 418 2 413 0.0 627
MS.gene047236.t1 AT1G09640 69.608 204 60 2 3 205 2 204 7.07e-97 291
MS.gene047236.t1 AT1G09640 88.732 71 7 1 349 418 195 265 1.00e-35 132
MS.gene047236.t1 AT2G30870 30.075 133 84 3 38 162 35 166 7.78e-13 67.8
MS.gene047236.t1 AT2G30860 29.371 143 94 2 27 162 24 166 1.70e-12 67.0
MS.gene047236.t1 AT3G62760 28.141 199 128 6 16 203 14 208 2.43e-12 66.6
MS.gene047236.t1 AT5G17220 28.655 171 113 3 42 204 40 209 1.30e-11 64.3

Find 117 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCTAATATCTTGCGGAAAT+TGG 0.099297 2.2:+73756162 None:intergenic
CTTTGGTTCATCTTTGGTTT+TGG 0.104573 2.2:+73756072 None:intergenic
CATTCATCCATGATAAATTT+TGG 0.113279 2.2:+73755452 None:intergenic
GTGTTTGATTTGCAGGATTC+TGG 0.117283 2.2:-73755688 MS.gene047236:intron
CACTTTCTTTGGTTCATCTT+TGG 0.187717 2.2:+73756066 None:intergenic
TCCTCCAAGTAAGATGATTT+TGG 0.192577 2.2:-73755960 MS.gene047236:CDS
TGGATCAGAGCCTCCATTTA+AGG 0.199866 2.2:-73755510 MS.gene047236:CDS
TTTGGAAAGATGCTTATAAT+TGG 0.211636 2.2:-73755530 MS.gene047236:CDS
TCTGATTTAGCCACTTTCTT+TGG 0.237195 2.2:+73756055 None:intergenic
AGGATTCTTGGGTTTGGGCT+TGG 0.251916 2.2:+73755994 None:intergenic
ACAACATCCAACTTGTATAT+TGG 0.252389 2.2:-73756265 MS.gene047236:CDS
CTCCTCTTCAACTTCTACTT+TGG 0.254637 2.2:+73756021 None:intergenic
GGATTCTTGGGTTTGGGCTT+GGG 0.262060 2.2:+73755995 None:intergenic
CCCAATTTCCGCAAGATATT+AGG 0.262248 2.2:-73756163 MS.gene047236:CDS
TTTCTTTCAGGTTCCGGTCT+TGG 0.279784 2.2:-73757615 MS.gene047236:intron
CACAACCCCTTCACCTTAAA+TGG 0.280661 2.2:+73755497 None:intergenic
CTGGCTCGCAAGTATGCTTT+TGG 0.282861 2.2:-73755548 MS.gene047236:CDS
ATTTGAGGGAGAGGCTCTTT+TGG 0.299039 2.2:-73755336 MS.gene047236:CDS
TTTATCAACATGAACCCTAT+TGG 0.305444 2.2:-73757714 MS.gene047236:CDS
TCCTCTTCAACTTCTACTTT+GGG 0.315309 2.2:+73756022 None:intergenic
TCAAGAGGATTCTTGGGTTT+GGG 0.320569 2.2:+73755989 None:intergenic
GTCAAGAGGATTCTTGGGTT+TGG 0.321823 2.2:+73755988 None:intergenic
ACAATACCTACCTTTCCAAT+AGG 0.324030 2.2:+73757699 None:intergenic
AAGAAGGTCAAGAGGATTCT+TGG 0.332287 2.2:+73755982 None:intergenic
TTGGGTCACAACAATTTGTT+TGG 0.342164 2.2:-73757441 MS.gene047236:CDS
GTTGGAAGCCATGGCGGCCT+TGG 0.343555 2.2:-73757949 None:intergenic
TTGTGACCCTGAACAAGGTT+GGG 0.356898 2.2:-73755592 MS.gene047236:CDS
CAGGTACAACTTCATGATAT+TGG 0.358542 2.2:+73756917 None:intergenic
CAATACCTACCTTTCCAATA+GGG 0.369972 2.2:+73757700 None:intergenic
CCTAATATCTTGCGGAAATT+GGG 0.374931 2.2:+73756163 None:intergenic
TGGATCATCTCTTATTGATC+AGG 0.386602 2.2:-73757421 MS.gene047236:intron
CTTTCCTGCAGTTGCCCGAT+TGG 0.389371 2.2:-73757460 MS.gene047236:intron
CTCATGTTGAGAGATACTTT+TGG 0.389806 2.2:-73756201 MS.gene047236:CDS
CATTTAAGGTGAAGGGGTTG+TGG 0.403530 2.2:-73755496 MS.gene047236:CDS
TGTTTGATTTGCAGGATTCT+GGG 0.410419 2.2:-73755687 MS.gene047236:intron
GAAACACCCATCTGAAAATC+AGG 0.417209 2.2:+73757753 None:intergenic
TTTCCTGCAGTTGCCCGATT+GGG 0.425435 2.2:-73757459 MS.gene047236:intron
AGAAGGTCAAGAGGATTCTT+GGG 0.433602 2.2:+73755983 None:intergenic
AGAGATTCCAAAATTTATCA+TGG 0.435888 2.2:-73755459 MS.gene047236:CDS
TATTGGTTTCGCTAAACTCT+TGG 0.436845 2.2:-73756248 MS.gene047236:CDS
TTTGTGACCCTGAACAAGGT+TGG 0.442505 2.2:-73755593 MS.gene047236:CDS
ATCCAGAGGGATACTCTCTC+TGG 0.443260 2.2:-73755655 MS.gene047236:CDS
CAAATTGTTGTGACCCAATC+GGG 0.445051 2.2:+73757446 None:intergenic
GGGTTCATGTTGATAAATTG+AGG 0.453213 2.2:+73757720 None:intergenic
GACCTCACGGAAGTTCCTCT+TGG 0.467302 2.2:+73755910 None:intergenic
AAAAGAGCCTCTCCCTCAAA+TGG 0.468428 2.2:+73755338 None:intergenic
ATTTCTGCAGAGGATGGATC+TGG 0.469227 2.2:-73755567 MS.gene047236:CDS
TAGCCTCAGTCTGCTTAACC+TGG 0.481958 2.2:+73756140 None:intergenic
TCATCCAAAATCATCTTACT+TGG 0.482283 2.2:+73755956 None:intergenic
CTCTCAAAGACAGGACCATC+AGG 0.483996 2.2:+73757587 None:intergenic
ACAAATTGTTGTGACCCAAT+CGG 0.484795 2.2:+73757445 None:intergenic
TGCTGAACCATTTGAGGGAG+AGG 0.491080 2.2:-73755345 MS.gene047236:CDS
TCCAAAATCATCTTACTTGG+AGG 0.494526 2.2:+73755959 None:intergenic
TGTGACCCTGAACAAGGTTG+GGG 0.494527 2.2:-73755591 MS.gene047236:CDS
GAAGATGCTGAACCATTTGA+GGG 0.499250 2.2:-73755350 MS.gene047236:CDS
ATCTGCTAAGCAGCAGCCTA+AGG 0.499718 2.2:-73756104 MS.gene047236:CDS
TGAAGATGCTGAACCATTTG+AGG 0.504616 2.2:-73755351 MS.gene047236:CDS
TTTCCGCAAGATATTAGGCC+AGG 0.506830 2.2:-73756158 MS.gene047236:CDS
GAGCCTCCATTTAAGGTGAA+GGG 0.508653 2.2:-73755503 MS.gene047236:CDS
GGTTCATGTTGATAAATTGA+GGG 0.510474 2.2:+73757721 None:intergenic
TGCTGCTTAGCAGATGGAAT+AGG 0.510683 2.2:+73756112 None:intergenic
ATATCTTGCGGAAATTGGGC+TGG 0.518090 2.2:+73756167 None:intergenic
TCATCTTACTTGGAGGAAGA+AGG 0.519375 2.2:+73755966 None:intergenic
TTCCGTGAGGTCGCAATCAA+AGG 0.519400 2.2:-73755899 MS.gene047236:intron
CTCACAATACCTACTTGGTC+GGG 0.524651 2.2:-73756315 MS.gene047236:CDS
AGCGAAACCAATATACAAGT+TGG 0.525950 2.2:+73756258 None:intergenic
CAAAGATGAACCAAAGAAAG+TGG 0.527852 2.2:-73756065 MS.gene047236:CDS
CGGCATATCCAAGTCTTGGC+AGG 0.531508 2.2:+73756898 None:intergenic
TTCGCTCAAGAGAGCACTTG+AGG 0.540956 2.2:-73756356 MS.gene047236:CDS
AACCAGAGAGAGTATCCCTC+TGG 0.541067 2.2:+73755653 None:intergenic
GCTCACAATACCTACTTGGT+CGG 0.543458 2.2:-73756316 MS.gene047236:CDS
TAGGCGGCATATCCAAGTCT+TGG 0.546133 2.2:+73756894 None:intergenic
TACCTTTGATTGCGACCTCA+CGG 0.549156 2.2:+73755897 None:intergenic
AGTAATGCCATTGCTCGCTA+TGG 0.549245 2.2:-73757566 MS.gene047236:intron
ACAACTTACTGGAGGAAAGT+AGG 0.550453 2.2:+73756875 None:intergenic
TTAGGCTGCTGCTTAGCAGA+TGG 0.550675 2.2:+73756106 None:intergenic
GCCCAAAGTAGAAGTTGAAG+AGG 0.556725 2.2:-73756023 MS.gene047236:CDS
AGCATCTTCAATCATCTGAC+TGG 0.558457 2.2:+73755364 None:intergenic
CTGATTGCTGCTGAATATGT+TGG 0.559330 2.2:-73757795 MS.gene047236:CDS
AAGTTGTACCTGCCAAGACT+TGG 0.560946 2.2:-73756906 MS.gene047236:CDS
AGAGCCTCCATTTAAGGTGA+AGG 0.562476 2.2:-73755504 MS.gene047236:CDS
TCTTGCTCACAATACCTACT+TGG 0.565718 2.2:-73756320 MS.gene047236:CDS
AATCAATCCACTGGTCAATG+TGG 0.571450 2.2:+73756961 None:intergenic
CAAAGTAGAAGTTGAAGAGG+AGG 0.579683 2.2:-73756020 MS.gene047236:CDS
TATACTAACCAAGGCCGCCA+TGG 0.580060 2.2:+73757941 None:intergenic
GACTCTACTCTAACACCAAG+AGG 0.583602 2.2:-73755925 MS.gene047236:CDS
CCATCAGGAGTTTCCAAGAC+CGG 0.583824 2.2:+73757602 None:intergenic
ATCAATCCACTGGTCAATGT+GGG 0.585165 2.2:+73756962 None:intergenic
CTTGGAGGAAGAAGGTCAAG+AGG 0.586796 2.2:+73755974 None:intergenic
CAGCATACCATAGCGAGCAA+TGG 0.587213 2.2:+73757559 None:intergenic
TTCATCAGAGATGTCAACCT+TGG 0.587509 2.2:+73755403 None:intergenic
TTCCGTTGTTGGGAGCGTGA+AGG 0.588156 2.2:+73757838 None:intergenic
CCGGTCTTGGAAACTCCTGA+TGG 0.589673 2.2:-73757602 MS.gene047236:CDS
GTTCATGTTGATAAATTGAG+GGG 0.590470 2.2:+73757722 None:intergenic
ATGGCATTACTCTCAAAGAC+AGG 0.591005 2.2:+73757578 None:intergenic
AGCCTCCATTTAAGGTGAAG+GGG 0.592740 2.2:-73755502 MS.gene047236:CDS
CAACATGAACCCTATTGGAA+AGG 0.595932 2.2:-73757709 MS.gene047236:intron
CTGGGACATGTATGATCCAG+AGG 0.600612 2.2:-73755669 MS.gene047236:CDS
ATCCTTCACGCTCCCAACAA+CGG 0.601253 2.2:-73757840 MS.gene047236:CDS
TTCAGGCCCACATTGACCAG+TGG 0.607287 2.2:-73756968 MS.gene047236:intron
TGCTTAGCAGATGGAATAGG+TGG 0.613551 2.2:+73756115 None:intergenic
CAAAAGTATCTCTCAACATG+AGG 0.614280 2.2:+73756202 None:intergenic
ATGTTGTTATAATGTCAGCA+AGG 0.622569 2.2:+73756281 None:intergenic
TAACCTGGCCTAATATCTTG+CGG 0.626332 2.2:+73756155 None:intergenic
TGTTGTTATAATGTCAGCAA+GGG 0.629008 2.2:+73756282 None:intergenic
CATGGATGAATGCTATGACA+TGG 0.631015 2.2:-73755441 MS.gene047236:CDS
GAAATTGGGCTGGTTGACCA+AGG 0.645258 2.2:+73756177 None:intergenic
GTGACCCTGAACAAGGTTGG+GGG 0.650105 2.2:-73755590 MS.gene047236:CDS
AACCCCTTCACCTTAAATGG+AGG 0.653598 2.2:+73755500 None:intergenic
ATGACATGGAGCTCTATGAG+TGG 0.662196 2.2:-73755427 MS.gene047236:CDS
TGGGACATGTATGATCCAGA+GGG 0.668464 2.2:-73755668 MS.gene047236:CDS
AACAGAATGCCCGACCAAGT+AGG 0.668821 2.2:+73756306 None:intergenic
CTCAACATGAGGAAACTCTG+AGG 0.714280 2.2:+73756213 None:intergenic
AGGCCAGGTTAAGCAGACTG+AGG 0.721460 2.2:-73756143 MS.gene047236:CDS
GGAGCTCTATGAGTGGACCA+AGG 0.731462 2.2:-73755420 MS.gene047236:CDS
ACTTACTGGAGGAAAGTAGG+CGG 0.737958 2.2:+73756878 None:intergenic
CACCAAGAGGAACTTCCGTG+AGG 0.751991 2.2:-73755912 MS.gene047236:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAAAATAGATTTAATCT+TGG + chr2.2:73756160-73756179 None:intergenic 10.0%
!!! AAGATTAAATCTATTTTAAT+TGG - chr2.2:73756159-73756178 MS.gene047236:CDS 10.0%
!! ACCATATAATAATAAAACAA+AGG - chr2.2:73755871-73755890 MS.gene047236:intron 15.0%
!! CAATTATATAATATTTGAGT+TGG - chr2.2:73756484-73756503 MS.gene047236:intron 15.0%
!!! TATCCTTTTTGATATTTATT+GGG - chr2.2:73756114-73756133 MS.gene047236:CDS 15.0%
!! AAAACATTTGTGAATAATTG+AGG + chr2.2:73756510-73756529 None:intergenic 20.0%
!! TAGACACAAATTTCAAAAAA+GGG + chr2.2:73756630-73756649 None:intergenic 20.0%
!! TATATATAAACCACATACAA+GGG + chr2.2:73756194-73756213 None:intergenic 20.0%
!! TGAAAAAAAAAAGAAACTAC+AGG + chr2.2:73757397-73757416 None:intergenic 20.0%
!! TTAGACACAAATTTCAAAAA+AGG + chr2.2:73756631-73756650 None:intergenic 20.0%
!! TTTCCCAATAAATATCAAAA+AGG + chr2.2:73756120-73756139 None:intergenic 20.0%
!!! AATATACTATTCTCTGTTTT+TGG - chr2.2:73757464-73757483 MS.gene047236:intron 20.0%
!!! GCCTTTGTTTTATTATTATA+TGG + chr2.2:73755875-73755894 None:intergenic 20.0%
!!! GTATCCTTTTTGATATTTAT+TGG - chr2.2:73756113-73756132 MS.gene047236:CDS 20.0%
!!! TATTTGAATTTTGTTGTTTC+AGG - chr2.2:73756270-73756289 MS.gene047236:CDS 20.0%
!!! TTTTCTGAAATCAATTAGTT+TGG - chr2.2:73756526-73756545 MS.gene047236:intron 20.0%
! AAGGGAAAATATTCAGTAAA+AGG + chr2.2:73756835-73756854 None:intergenic 25.0%
! AGAGAATAGTATATTTCCAA+AGG + chr2.2:73757459-73757478 None:intergenic 25.0%
! AGAGATTCCAAAATTTATCA+TGG - chr2.2:73757796-73757815 MS.gene047236:CDS 25.0%
! AGGGAAAATATTCAGTAAAA+GGG + chr2.2:73756834-73756853 None:intergenic 25.0%
! CTATATATAAACCACATACA+AGG + chr2.2:73756195-73756214 None:intergenic 25.0%
! GTATGTGGTTTATATATAGA+GGG - chr2.2:73756196-73756215 MS.gene047236:CDS 25.0%
! TAATAATCAACAACAAGTAG+TGG + chr2.2:73755379-73755398 None:intergenic 25.0%
! TATAATTGCTATCAATACTC+AGG + chr2.2:73756472-73756491 None:intergenic 25.0%
! TATTATTCCTTCTTTCTTTC+AGG - chr2.2:73755628-73755647 MS.gene047236:CDS 25.0%
! TCAGACACAAATTTCAAAAA+GGG + chr2.2:73756853-73756872 None:intergenic 25.0%
! TGAAATTTGTGTCTAAATGA+AGG - chr2.2:73756635-73756654 MS.gene047236:intron 25.0%
! TGAAATTTGTGTCTGAATAA+AGG - chr2.2:73756857-73756876 MS.gene047236:intron 25.0%
! TGTATGTGGTTTATATATAG+AGG - chr2.2:73756195-73756214 MS.gene047236:CDS 25.0%
! TTCAGACACAAATTTCAAAA+AGG + chr2.2:73756854-73756873 None:intergenic 25.0%
! TTTGGAAAGATGCTTATAAT+TGG - chr2.2:73757725-73757744 MS.gene047236:CDS 25.0%
!! ATCTAAACTAGAATCTTTTC+TGG + chr2.2:73756000-73756019 None:intergenic 25.0%
!! CATTCATCCATGATAAATTT+TGG + chr2.2:73757806-73757825 None:intergenic 25.0%
!! CTTGCTGAATATTTTCAAAA+GGG + chr2.2:73756706-73756725 None:intergenic 25.0%
!! TTGCTGAATATTTTCAAAAG+GGG + chr2.2:73756705-73756724 None:intergenic 25.0%
!!! ACTTGCTGAATATTTTCAAA+AGG + chr2.2:73756707-73756726 None:intergenic 25.0%
AATCAGAGAAAAAGTAAGTG+TGG + chr2.2:73756035-73756054 None:intergenic 30.0%
ACAACATCCAACTTGTATAT+TGG - chr2.2:73756990-73757009 MS.gene047236:intron 30.0%
ATAGATTTAATCTTGGCAGT+TGG + chr2.2:73756153-73756172 None:intergenic 30.0%
ATGTTGTTATAATGTCAGCA+AGG + chr2.2:73756977-73756996 None:intergenic 30.0%
GGGAAAATATTCAGTAAAAG+GGG + chr2.2:73756833-73756852 None:intergenic 30.0%
TATAGAGGGTAAATTTGCAA+AGG - chr2.2:73756210-73756229 MS.gene047236:CDS 30.0%
TCATCCAAAATCATCTTACT+TGG + chr2.2:73757302-73757321 None:intergenic 30.0%
TGATTGTAGCTTATATCCTT+TGG - chr2.2:73757440-73757459 MS.gene047236:CDS 30.0%
TGTTGTTATAATGTCAGCAA+GGG + chr2.2:73756976-73756995 None:intergenic 30.0%
TTTATCAACATGAACCCTAT+TGG - chr2.2:73755541-73755560 MS.gene047236:CDS 30.0%
! ATCTCTTTTAACATCTCAGT+TGG - chr2.2:73756768-73756787 MS.gene047236:intron 30.0%
! GGTTCATGTTGATAAATTGA+GGG + chr2.2:73755537-73755556 None:intergenic 30.0%
! GTTCATGTTGATAAATTGAG+GGG + chr2.2:73755536-73755555 None:intergenic 30.0%
! TGCTGAATATTTTCAAAAGG+GGG + chr2.2:73756704-73756723 None:intergenic 30.0%
!! AACATTTTTGTTTCCGTTGT+TGG + chr2.2:73755431-73755450 None:intergenic 30.0%
!! ACATTTTTGTTTCCGTTGTT+GGG + chr2.2:73755430-73755449 None:intergenic 30.0%
!!! TTTGGTTCATCTTTGGTTTT+GGG + chr2.2:73757185-73757204 None:intergenic 30.0%
AATAACTTCCATGAGAACAC+AGG + chr2.2:73756254-73756273 None:intergenic 35.0%
ACAAATTGTTGTGACCCAAT+CGG + chr2.2:73755813-73755832 None:intergenic 35.0%
ACAATACCTACCTTTCCAAT+AGG + chr2.2:73755559-73755578 None:intergenic 35.0%
AGCGAAACCAATATACAAGT+TGG + chr2.2:73757000-73757019 None:intergenic 35.0%
ATAACTTCCATGAGAACACA+GGG + chr2.2:73756253-73756272 None:intergenic 35.0%
ATAATAAAACAAAGGCCGTG+TGG - chr2.2:73755879-73755898 MS.gene047236:intron 35.0%
ATCTCTTCTAACATCTCAGA+TGG - chr2.2:73756566-73756585 MS.gene047236:intron 35.0%
ATTTAATCTTGGCAGTTGGT+TGG + chr2.2:73756149-73756168 None:intergenic 35.0%
CAAAGATGAACCAAAGAAAG+TGG - chr2.2:73757190-73757209 MS.gene047236:intron 35.0%
CAATACCTACCTTTCCAATA+GGG + chr2.2:73755558-73755577 None:intergenic 35.0%
CAGGTACAACTTCATGATAT+TGG + chr2.2:73756341-73756360 None:intergenic 35.0%
CCTAATATCTTGCGGAAATT+GGG + chr2.2:73757095-73757114 None:intergenic 35.0%
GAGAAGAAAAATCAATCCAC+TGG + chr2.2:73756306-73756325 None:intergenic 35.0%
GAGAGTGAATATACTAACCA+AGG + chr2.2:73755326-73755345 None:intergenic 35.0%
GAGATGTTAGAAGAGATACA+TGG + chr2.2:73756563-73756582 None:intergenic 35.0%
TCCAAAATCATCTTACTTGG+AGG + chr2.2:73757299-73757318 None:intergenic 35.0%
TCCTCTTCAACTTCTACTTT+GGG + chr2.2:73757236-73757255 None:intergenic 35.0%
TGGATCATCTCTTATTGATC+AGG - chr2.2:73755834-73755853 MS.gene047236:intron 35.0%
TTGTGTCTGAATAAAGGTAG+AGG - chr2.2:73756863-73756882 MS.gene047236:intron 35.0%
TTTGTGTCTAAATGAAGGAC+TGG - chr2.2:73756640-73756659 MS.gene047236:intron 35.0%
! AACTTCTACTTTGGGCTTTT+CGG + chr2.2:73757228-73757247 None:intergenic 35.0%
! AGAAGAGATACATGGATTGT+TGG + chr2.2:73756555-73756574 None:intergenic 35.0%
! CACTTTCTTTGGTTCATCTT+TGG + chr2.2:73757192-73757211 None:intergenic 35.0%
! CTCATGTTGAGAGATACTTT+TGG - chr2.2:73757054-73757073 MS.gene047236:intron 35.0%
! GCTGAATATTTTCAAAAGGG+GGG + chr2.2:73756703-73756722 None:intergenic 35.0%
! GGGTTCATGTTGATAAATTG+AGG + chr2.2:73755538-73755557 None:intergenic 35.0%
! GTAAGATGATTTTGGATGAC+TGG - chr2.2:73757303-73757322 MS.gene047236:intron 35.0%
! TCCTCCAAGTAAGATGATTT+TGG - chr2.2:73757295-73757314 MS.gene047236:intron 35.0%
! TCTGATTTAGCCACTTTCTT+TGG + chr2.2:73757203-73757222 None:intergenic 35.0%
! TTCAAGCTTTTGTAGCTTCT+AGG - chr2.2:73757413-73757432 MS.gene047236:intron 35.0%
! TTGACTCCTGATTTTCAGAT+GGG - chr2.2:73755496-73755515 MS.gene047236:CDS 35.0%
! TTGGGTCACAACAATTTGTT+TGG - chr2.2:73755814-73755833 MS.gene047236:intron 35.0%
!! AATTCCTGTGTTTGATTTGC+AGG - chr2.2:73757560-73757579 MS.gene047236:intron 35.0%
!! ATTGACTCCTGATTTTCAGA+TGG - chr2.2:73755495-73755514 MS.gene047236:CDS 35.0%
!! CAAAAGTATCTCTCAACATG+AGG + chr2.2:73757056-73757075 None:intergenic 35.0%
!! CTTTGGTTCATCTTTGGTTT+TGG + chr2.2:73757186-73757205 None:intergenic 35.0%
!! GATAAATTGAGGGGTTTTGT+TGG + chr2.2:73755527-73755546 None:intergenic 35.0%
!! GTGGATTGATTTTTCTTCTC+TGG - chr2.2:73756306-73756325 MS.gene047236:CDS 35.0%
!! TATTGGTTTCGCTAAACTCT+TGG - chr2.2:73757007-73757026 MS.gene047236:intron 35.0%
!! TGTTTGATTTGCAGGATTCT+GGG - chr2.2:73757568-73757587 MS.gene047236:CDS 35.0%
!!! ATTTTGGAATCTCTTGTCCA+CGG + chr2.2:73757790-73757809 None:intergenic 35.0%
AAACATGGCACACAACTTAC+TGG + chr2.2:73756394-73756413 None:intergenic 40.0%
AAGAAGGTCAAGAGGATTCT+TGG + chr2.2:73757276-73757295 None:intergenic 40.0%
AATCAATCCACTGGTCAATG+TGG + chr2.2:73756297-73756316 None:intergenic 40.0%
ACAACTTACTGGAGGAAAGT+AGG + chr2.2:73756383-73756402 None:intergenic 40.0%
AGAAGGTCAAGAGGATTCTT+GGG + chr2.2:73757275-73757294 None:intergenic 40.0%
AGCATCTTCAATCATCTGAC+TGG + chr2.2:73757894-73757913 None:intergenic 40.0%
ATCAATCCACTGGTCAATGT+GGG + chr2.2:73756296-73756315 None:intergenic 40.0%
ATGAACCCTATTGGAAAGGT+AGG - chr2.2:73755550-73755569 MS.gene047236:CDS 40.0%
ATGGCATTACTCTCAAAGAC+AGG + chr2.2:73755680-73755699 None:intergenic 40.0%
CAAAGTAGAAGTTGAAGAGG+AGG - chr2.2:73757235-73757254 MS.gene047236:intron 40.0%
CAAATTGTTGTGACCCAATC+GGG + chr2.2:73755812-73755831 None:intergenic 40.0%
CAACATGAACCCTATTGGAA+AGG - chr2.2:73755546-73755565 MS.gene047236:CDS 40.0%
CATGGATGAATGCTATGACA+TGG - chr2.2:73757814-73757833 MS.gene047236:CDS 40.0%
CCCAATTTCCGCAAGATATT+AGG - chr2.2:73757092-73757111 MS.gene047236:intron 40.0%
CTCCTCTTCAACTTCTACTT+TGG + chr2.2:73757237-73757256 None:intergenic 40.0%
CTGATTGCTGCTGAATATGT+TGG - chr2.2:73755460-73755479 MS.gene047236:CDS 40.0%
GAAACACCCATCTGAAAATC+AGG + chr2.2:73755505-73755524 None:intergenic 40.0%
GAAGATGCTGAACCATTTGA+GGG - chr2.2:73757905-73757924 MS.gene047236:intron 40.0%
GAATCCTGCAAATCAAACAC+AGG + chr2.2:73757567-73757586 None:intergenic 40.0%
GCCTAATATCTTGCGGAAAT+TGG + chr2.2:73757096-73757115 None:intergenic 40.0%
TAACCTGGCCTAATATCTTG+CGG + chr2.2:73757103-73757122 None:intergenic 40.0%
TCAAGAGGATTCTTGGGTTT+GGG + chr2.2:73757269-73757288 None:intergenic 40.0%
TCATCTTACTTGGAGGAAGA+AGG + chr2.2:73757292-73757311 None:intergenic 40.0%
TCTTGCTCACAATACCTACT+TGG - chr2.2:73756935-73756954 MS.gene047236:CDS 40.0%
TGAAGATGCTGAACCATTTG+AGG - chr2.2:73757904-73757923 MS.gene047236:intron 40.0%
TTCAGTAAAAGGGGAAGAGT+TGG + chr2.2:73756824-73756843 None:intergenic 40.0%
TTCATCAGAGATGTCAACCT+TGG + chr2.2:73757855-73757874 None:intergenic 40.0%
TTGAAAACCAAGAGACCACA+CGG + chr2.2:73755897-73755916 None:intergenic 40.0%
! ACTTCTACTTTGGGCTTTTC+GGG + chr2.2:73757227-73757246 None:intergenic 40.0%
! CACAGGGATATGCAATCTTT+TGG + chr2.2:73756237-73756256 None:intergenic 40.0%
! TATTTTCAAAAGGGGGGAGT+TGG + chr2.2:73756697-73756716 None:intergenic 40.0%
! TGAGAGATACTTTTGGACCT+TGG - chr2.2:73757061-73757080 MS.gene047236:intron 40.0%
! TTCTTTTGTGACCCTGAACA+AGG - chr2.2:73757658-73757677 MS.gene047236:intron 40.0%
!! GTGTTTGATTTGCAGGATTC+TGG - chr2.2:73757567-73757586 MS.gene047236:CDS 40.0%
!! TCCTTCTTTCTTTCAGGTTC+CGG - chr2.2:73755634-73755653 MS.gene047236:CDS 40.0%
AAAAGAGCCTCTCCCTCAAA+TGG + chr2.2:73757920-73757939 None:intergenic 45.0%
AAATCCCCCAACCTTGTTCA+GGG + chr2.2:73757672-73757691 None:intergenic 45.0%
AACCCCTTCACCTTAAATGG+AGG + chr2.2:73757758-73757777 None:intergenic 45.0%
AAGTTGTACCTGCCAAGACT+TGG - chr2.2:73756349-73756368 MS.gene047236:CDS 45.0%
ACCGGAACCTGAAAGAAAGA+AGG + chr2.2:73755638-73755657 None:intergenic 45.0%
ACTTACTGGAGGAAAGTAGG+CGG + chr2.2:73756380-73756399 None:intergenic 45.0%
AGTAATGCCATTGCTCGCTA+TGG - chr2.2:73755689-73755708 MS.gene047236:intron 45.0%
ATATCTTGCGGAAATTGGGC+TGG + chr2.2:73757091-73757110 None:intergenic 45.0%
ATGACATGGAGCTCTATGAG+TGG - chr2.2:73757828-73757847 MS.gene047236:CDS 45.0%
ATTTCTGCAGAGGATGGATC+TGG - chr2.2:73757688-73757707 MS.gene047236:intron 45.0%
CACAACCCCTTCACCTTAAA+TGG + chr2.2:73757761-73757780 None:intergenic 45.0%
CTCAACATGAGGAAACTCTG+AGG + chr2.2:73757045-73757064 None:intergenic 45.0%
CTCACAATACCTACTTGGTC+GGG - chr2.2:73756940-73756959 MS.gene047236:CDS 45.0%
GACTCTACTCTAACACCAAG+AGG - chr2.2:73757330-73757349 MS.gene047236:intron 45.0%
GCCCAAAGTAGAAGTTGAAG+AGG - chr2.2:73757232-73757251 MS.gene047236:intron 45.0%
GCTCACAATACCTACTTGGT+CGG - chr2.2:73756939-73756958 MS.gene047236:CDS 45.0%
GTCAAGAGGATTCTTGGGTT+TGG + chr2.2:73757270-73757289 None:intergenic 45.0%
TACCTTTGATTGCGACCTCA+CGG + chr2.2:73757361-73757380 None:intergenic 45.0%
TGCATATCCCTGTGTTCTCA+TGG - chr2.2:73756243-73756262 MS.gene047236:CDS 45.0%
TGCTTAGCAGATGGAATAGG+TGG + chr2.2:73757143-73757162 None:intergenic 45.0%
TGGATCAGAGCCTCCATTTA+AGG - chr2.2:73757745-73757764 MS.gene047236:CDS 45.0%
TGGGACATGTATGATCCAGA+GGG - chr2.2:73757587-73757606 MS.gene047236:CDS 45.0%
TTGTGACCCTGAACAAGGTT+GGG - chr2.2:73757663-73757682 MS.gene047236:intron 45.0%
TTTCCGCAAGATATTAGGCC+AGG - chr2.2:73757097-73757116 MS.gene047236:intron 45.0%
TTTGTGACCCTGAACAAGGT+TGG - chr2.2:73757662-73757681 MS.gene047236:intron 45.0%
! AGAGCCTCCATTTAAGGTGA+AGG - chr2.2:73757751-73757770 MS.gene047236:CDS 45.0%
! AGCCTCCATTTAAGGTGAAG+GGG - chr2.2:73757753-73757772 MS.gene047236:CDS 45.0%
! ATTTGAGGGAGAGGCTCTTT+TGG - chr2.2:73757919-73757938 MS.gene047236:intron 45.0%
! CATTTAAGGTGAAGGGGTTG+TGG - chr2.2:73757759-73757778 MS.gene047236:CDS 45.0%
! GAGCCTCCATTTAAGGTGAA+GGG - chr2.2:73757752-73757771 MS.gene047236:CDS 45.0%
! GTTGAAGCATCCCTTGTATG+TGG - chr2.2:73756181-73756200 MS.gene047236:CDS 45.0%
! TGCTGCTTAGCAGATGGAAT+AGG + chr2.2:73757146-73757165 None:intergenic 45.0%
!! GGTCGCAATCAAAGGTATTG+TGG - chr2.2:73757364-73757383 MS.gene047236:intron 45.0%
!! GTTTTGGGCTTAGACTCCTT+AGG + chr2.2:73757170-73757189 None:intergenic 45.0%
!! TTTCTTTCAGGTTCCGGTCT+TGG - chr2.2:73755640-73755659 MS.gene047236:CDS 45.0%
!! AAAAAAAAAAAAAAAAAACA+TGG + chr2.2:73756409-73756428 None:intergenic 5.0%
AACAGAATGCCCGACCAAGT+AGG + chr2.2:73756952-73756971 None:intergenic 50.0%
AGGATTCTTGGGTTTGGGCT+TGG + chr2.2:73757264-73757283 None:intergenic 50.0%
ATCCAGAGGGATACTCTCTC+TGG - chr2.2:73757600-73757619 MS.gene047236:CDS 50.0%
ATCCTTCACGCTCCCAACAA+CGG - chr2.2:73755415-73755434 MS.gene047236:CDS 50.0%
ATCTGCTAAGCAGCAGCCTA+AGG - chr2.2:73757151-73757170 MS.gene047236:intron 50.0%
CAGCATACCATAGCGAGCAA+TGG + chr2.2:73755699-73755718 None:intergenic 50.0%
CATGGCACACAACTTACTGG+AGG + chr2.2:73756391-73756410 None:intergenic 50.0%
CCATCAGGAGTTTCCAAGAC+CGG + chr2.2:73755656-73755675 None:intergenic 50.0%
CTCTCAAAGACAGGACCATC+AGG + chr2.2:73755671-73755690 None:intergenic 50.0%
CTGGGACATGTATGATCCAG+AGG - chr2.2:73757586-73757605 MS.gene047236:CDS 50.0%
CTTGGAGGAAGAAGGTCAAG+AGG + chr2.2:73757284-73757303 None:intergenic 50.0%
GAAATCCCCCAACCTTGTTC+AGG + chr2.2:73757673-73757692 None:intergenic 50.0%
GGATTCTTGGGTTTGGGCTT+GGG + chr2.2:73757263-73757282 None:intergenic 50.0%
TAGCCTCAGTCTGCTTAACC+TGG + chr2.2:73757118-73757137 None:intergenic 50.0%
TAGGCGGCATATCCAAGTCT+TGG + chr2.2:73756364-73756383 None:intergenic 50.0%
TGCTGAACCATTTGAGGGAG+AGG - chr2.2:73757910-73757929 MS.gene047236:intron 50.0%
TGTGACCCTGAACAAGGTTG+GGG - chr2.2:73757664-73757683 MS.gene047236:intron 50.0%
TTCCGTGAGGTCGCAATCAA+AGG - chr2.2:73757356-73757375 MS.gene047236:intron 50.0%
TTTCCTGCAGTTGCCCGATT+GGG - chr2.2:73755796-73755815 MS.gene047236:intron 50.0%
! TTAGGCTGCTGCTTAGCAGA+TGG + chr2.2:73757152-73757171 None:intergenic 50.0%
!! AACCAGAGAGAGTATCCCTC+TGG + chr2.2:73757605-73757624 None:intergenic 50.0%
!! CTGGCTCGCAAGTATGCTTT+TGG - chr2.2:73757707-73757726 MS.gene047236:intron 50.0%
!! GAAATTGGGCTGGTTGACCA+AGG + chr2.2:73757081-73757100 None:intergenic 50.0%
!! TTCGCTCAAGAGAGCACTTG+AGG - chr2.2:73756899-73756918 MS.gene047236:CDS 50.0%
ACAAAGGCCGTGTGGTCTCT+TGG - chr2.2:73755887-73755906 MS.gene047236:intron 55.0%
AGGCCAGGTTAAGCAGACTG+AGG - chr2.2:73757112-73757131 MS.gene047236:intron 55.0%
AGGTTGGGGGATTTCTGCAG+AGG - chr2.2:73757678-73757697 MS.gene047236:intron 55.0%
CACCAAGAGGAACTTCCGTG+AGG - chr2.2:73757343-73757362 MS.gene047236:intron 55.0%
CCGGTCTTGGAAACTCCTGA+TGG - chr2.2:73755653-73755672 MS.gene047236:CDS 55.0%
CGGCATATCCAAGTCTTGGC+AGG + chr2.2:73756360-73756379 None:intergenic 55.0%
CTTTCCTGCAGTTGCCCGAT+TGG - chr2.2:73755795-73755814 MS.gene047236:intron 55.0%
GACCTCACGGAAGTTCCTCT+TGG + chr2.2:73757348-73757367 None:intergenic 55.0%
GGAGCTCTATGAGTGGACCA+AGG - chr2.2:73757835-73757854 MS.gene047236:CDS 55.0%
GTGACCCTGAACAAGGTTGG+GGG - chr2.2:73757665-73757684 MS.gene047236:intron 55.0%
TGGGGGATTTCTGCAGAGGA+TGG - chr2.2:73757682-73757701 MS.gene047236:intron 55.0%
TTCAGGCCCACATTGACCAG+TGG - chr2.2:73756287-73756306 MS.gene047236:CDS 55.0%
! AAGGGGTTGTGGCTTTTCCG+TGG - chr2.2:73757770-73757789 MS.gene047236:CDS 55.0%
! TTCCGTTGTTGGGAGCGTGA+AGG + chr2.2:73755420-73755439 None:intergenic 55.0%
TGACCCAATCGGGCAACTGC+AGG + chr2.2:73755802-73755821 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 73755316 73757961 73755316 ID=MS.gene047236
chr2.2 mRNA 73755316 73757961 73755316 ID=MS.gene047236.t1;Parent=MS.gene047236
chr2.2 exon 73757950 73757961 73757950 ID=MS.gene047236.t1.exon1;Parent=MS.gene047236.t1
chr2.2 CDS 73757950 73757961 73757950 ID=cds.MS.gene047236.t1;Parent=MS.gene047236.t1
chr2.2 exon 73757710 73757862 73757710 ID=MS.gene047236.t1.exon2;Parent=MS.gene047236.t1
chr2.2 CDS 73757710 73757862 73757710 ID=cds.MS.gene047236.t1;Parent=MS.gene047236.t1
chr2.2 exon 73757567 73757627 73757567 ID=MS.gene047236.t1.exon3;Parent=MS.gene047236.t1
chr2.2 CDS 73757567 73757627 73757567 ID=cds.MS.gene047236.t1;Parent=MS.gene047236.t1
chr2.2 exon 73757422 73757471 73757422 ID=MS.gene047236.t1.exon4;Parent=MS.gene047236.t1
chr2.2 CDS 73757422 73757471 73757422 ID=cds.MS.gene047236.t1;Parent=MS.gene047236.t1
chr2.2 exon 73756884 73756985 73756884 ID=MS.gene047236.t1.exon5;Parent=MS.gene047236.t1
chr2.2 CDS 73756884 73756985 73756884 ID=cds.MS.gene047236.t1;Parent=MS.gene047236.t1
chr2.2 exon 73755900 73756398 73755900 ID=MS.gene047236.t1.exon6;Parent=MS.gene047236.t1
chr2.2 CDS 73755900 73756398 73755900 ID=cds.MS.gene047236.t1;Parent=MS.gene047236.t1
chr2.2 exon 73755316 73755695 73755316 ID=MS.gene047236.t1.exon7;Parent=MS.gene047236.t1
chr2.2 CDS 73755316 73755695 73755316 ID=cds.MS.gene047236.t1;Parent=MS.gene047236.t1
Gene Sequence

>MS.gene047236

ATGGCGGCCTTGGTTAGTATATTCACTCTCTCTTTGTTCTTCGTTTTCTATTTTCAATTACCACTACTTGTTGTTGATTATTATGATTTTACAATTTAGATCCTTCACGCTCCCAACAACGGAAACAAAAATGTTTACAAAACTCTGATTGCTGCTGAATATGTTGGTGTTCAAGTTCAATTGACTCCTGATTTTCAGATGGGTGTTTCCAACAAAACCCCTCAATTTATCAACATGAACCCTATTGGAAAGGTAGGTATTGTTTATTATTCTTTCTTTCATTATATAATCAATTTCTATCTAAATTATTATTATTATTCCTTCTTTCTTTCAGGTTCCGGTCTTGGAAACTCCTGATGGTCCTGTCTTTGAGAGTAATGCCATTGCTCGCTATGGTATGCTGCTTTTTTCACTTTACTTGATGTTACATTATCTCTCTTTTTTTTTTTTTTTGTTCATTTACTCATTCTTGTAACTTACTTTCCTGCAGTTGCCCGATTGGGTCACAACAATTTGTTTGGATCATCTCTTATTGATCAGGTAACTTACATGAATACCATATAATAATAAAACAAAGGCCGTGTGGTCTCTTGGTTTTCAATTCTAAATTAACTGTTACTACATTCAAATATTTTTATATTTAATTTATTAAGTGAAAAAAACTGTTTTCAAGTAATTTTGCCAGAAAAGATTCTAGTTTAGATGAAATCTTAAATCCACACTTACTTTTTCTCTGATTACTATTGCAATTGAAAAAAAAAACTGTTTTCATATGAAACTTAATTAAATTTACAGAAGTATCCTTTTTGATATTTATTGGGAAAATTCAACCAACCAACTGCCAAGATTAAATCTATTTTAATTGGTTGAAGCATCCCTTGTATGTGGTTTATATATAGAGGGTAAATTTGCAAAGGACCAAAAGATTGCATATCCCTGTGTTCTCATGGAAGTTATTTGAATTTTGTTGTTTCAGGCCCACATTGACCAGTGGATTGATTTTTCTTCTCTGGAGATTGATGCCAATATCATGAAGTTGTACCTGCCAAGACTTGGATATGCCGCCTACTTTCCTCCAGTAAGTTGTGTGCCATGTTTTTTTTTTTTTTTTTTCAATATTAAATTGTTTTTTATCTTGTTTGAAGTTTCTAGTCCTGAGTATTGATAGCAATTATATAATATTTGAGTTGGCCTCAATTATTCACAAATGTTTTCTGAAATCAATTAGTTTGGTTACCAACAATCCATGTATCTCTTCTAACATCTCAGATGGTACACTGTTATTATTCGAGTCTTTGCAATACAAACTTTCCCTTTTTTGAAATTTGTGTCTAAATGAAGGACTGGTAAATCAATTACTCAAAGTGTTTTTGCAATACCAACTCCCCCCTTTTGAAAATATTCAGCAAGTGCTTTTTGAAATCATTTAGTTTGATCATTAACAATCAATGTATCTCTTTTAACATCTCAGTTGGTACATGTTTATTATTTGCGTTTTTACAATACCAACTCTTCCCCTTTTACTGAATATTTTCCCTTTTTGAAATTTGTGTCTGAATAAAGGTAGAGGAGAATGCAATTTCTTCGCTCAAGAGAGCACTTGAGGCATTGAACACTCATCTTGCTCACAATACCTACTTGGTCGGGCATTCTGTTACCCTTGCTGACATTATAACAACATCCAACTTGTATATTGGTTTCGCTAAACTCTTGGTTAAAAGCTTCACCTCAGAGTTTCCTCATGTTGAGAGATACTTTTGGACCTTGGTCAACCAGCCCAATTTCCGCAAGATATTAGGCCAGGTTAAGCAGACTGAGGCTATGCCACCTATTCCATCTGCTAAGCAGCAGCCTAAGGAGTCTAAGCCCAAAACCAAAGATGAACCAAAGAAAGTGGCTAAATCAGAGCCCGAAAAGCCCAAAGTAGAAGTTGAAGAGGAGGCACCCAAGCCCAAACCCAAGAATCCTCTTGACCTTCTTCCTCCAAGTAAGATGATTTTGGATGACTGGAAAAGACTCTACTCTAACACCAAGAGGAACTTCCGTGAGGTCGCAATCAAAGGTATTGTGGTCACATTCCTGTAGTTTCTTTTTTTTTTCAAGCTTTTGTAGCTTCTAGGATTATGATTGTAGCTTATATCCTTTGGAAATATACTATTCTCTGTTTTTGGAAACTTGATTCTTTTTATCATGACTGTACTGTATCAAAGAATGTCAAAATTTGTTAATGAGATTCAATGATCTAATTCCTGTGTTTGATTTGCAGGATTCTGGGACATGTATGATCCAGAGGGATACTCTCTCTGGTTCTGTGATTACAAATACAATGATGAGAACACTGTTTCTTTTGTGACCCTGAACAAGGTTGGGGGATTTCTGCAGAGGATGGATCTGGCTCGCAAGTATGCTTTTGGAAAGATGCTTATAATTGGATCAGAGCCTCCATTTAAGGTGAAGGGGTTGTGGCTTTTCCGTGGACAAGAGATTCCAAAATTTATCATGGATGAATGCTATGACATGGAGCTCTATGAGTGGACCAAGGTTGACATCTCTGATGAAGTTCAGAAAGAGCGTGCCAGTCAGATGATTGAAGATGCTGAACCATTTGAGGGAGAGGCTCTTTTGGATGCCAAGTGCTTTAAGTGA
Protein sequence

>MS.gene047236.t1

MAALILHAPNNGNKNVYKTLIAAEYVGVQVQLTPDFQMGVSNKTPQFINMNPIGKVPVLETPDGPVFESNAIARYVARLGHNNLFGSSLIDQAHIDQWIDFSSLEIDANIMKLYLPRLGYAAYFPPVEENAISSLKRALEALNTHLAHNTYLVGHSVTLADIITTSNLYIGFAKLLVKSFTSEFPHVERYFWTLVNQPNFRKILGQVKQTEAMPPIPSAKQQPKESKPKTKDEPKKVAKSEPEKPKVEVEEEAPKPKPKNPLDLLPPSKMILDDWKRLYSNTKRNFREVAIKGFWDMYDPEGYSLWFCDYKYNDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLIIGSEPPFKVKGLWLFRGQEIPKFIMDECYDMELYEWTKVDISDEVQKERASQMIEDAEPFEGEALLDAKCFK