Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048337.t1 | XP_013446118.1 | 99.4 | 348 | 2 | 0 | 1 | 348 | 1 | 348 | 1.00E-181 | 646 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048337.t1 | Q38942 | 84.8 | 349 | 52 | 1 | 1 | 348 | 1 | 349 | 3.6e-159 | 562.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048337.t1 | A0A072U2U6 | 99.4 | 348 | 2 | 0 | 1 | 348 | 1 | 348 | 7.4e-182 | 646.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene048337 | MS.gene55320 | PPI |
| MS.gene00617 | MS.gene048337 | PPI |
| MS.gene072058 | MS.gene048337 | PPI |
| MS.gene046944 | MS.gene048337 | PPI |
| MS.gene048337 | MS.gene85897 | PPI |
| MS.gene048337 | MS.gene066387 | PPI |
| MS.gene004979 | MS.gene048337 | PPI |
| MS.gene92360 | MS.gene048337 | PPI |
| MS.gene022537 | MS.gene048337 | PPI |
| MS.gene79186 | MS.gene048337 | PPI |
| MS.gene065809 | MS.gene048337 | PPI |
| MS.gene024963 | MS.gene048337 | PPI |
| MS.gene000251 | MS.gene048337 | PPI |
| MS.gene048337 | MS.gene047200 | PPI |
| MS.gene048337 | MS.gene046944 | PPI |
| MS.gene07517 | MS.gene048337 | PPI |
| MS.gene048337 | MS.gene61568 | PPI |
| MS.gene060071 | MS.gene048337 | PPI |
| MS.gene86831 | MS.gene048337 | PPI |
| MS.gene048337 | MS.gene40528 | PPI |
| MS.gene060069 | MS.gene048337 | PPI |
| MS.gene79188 | MS.gene048337 | PPI |
| MS.gene53114 | MS.gene048337 | PPI |
| MS.gene62514 | MS.gene048337 | PPI |
| MS.gene060067 | MS.gene048337 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048337.t1 | MTR_8g068875 | 99.138 | 348 | 3 | 0 | 1 | 348 | 1 | 348 | 0.0 | 733 |
| MS.gene048337.t1 | MTR_2g008410 | 85.075 | 335 | 49 | 1 | 15 | 348 | 17 | 351 | 0.0 | 626 |
| MS.gene048337.t1 | MTR_1g492860 | 86.207 | 348 | 46 | 1 | 1 | 348 | 1 | 346 | 0.0 | 626 |
| MS.gene048337.t1 | MTR_2g008410 | 80.597 | 335 | 46 | 2 | 15 | 348 | 17 | 333 | 0.0 | 582 |
| MS.gene048337.t1 | MTR_2g008410 | 88.070 | 285 | 34 | 0 | 64 | 348 | 2 | 286 | 0.0 | 555 |
| MS.gene048337.t1 | MTR_2g008410 | 82.807 | 285 | 31 | 1 | 64 | 348 | 2 | 268 | 0.0 | 510 |
| MS.gene048337.t1 | MTR_5g005120 | 34.524 | 336 | 197 | 7 | 18 | 342 | 15 | 338 | 2.78e-63 | 205 |
| MS.gene048337.t1 | MTR_5g043000 | 27.586 | 319 | 216 | 7 | 8 | 319 | 2 | 312 | 1.65e-28 | 113 |
| MS.gene048337.t1 | MTR_2g436510 | 24.516 | 310 | 191 | 11 | 13 | 309 | 159 | 438 | 2.85e-13 | 70.9 |
| MS.gene048337.t1 | MTR_6g004040 | 34.211 | 114 | 70 | 1 | 28 | 141 | 105 | 213 | 7.95e-12 | 67.0 |
| MS.gene048337.t1 | MTR_2g012630 | 33.333 | 114 | 71 | 1 | 28 | 141 | 104 | 212 | 1.24e-11 | 66.2 |
| MS.gene048337.t1 | MTR_2g012630 | 33.333 | 114 | 71 | 1 | 28 | 141 | 104 | 212 | 1.39e-11 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048337.t1 | AT1G80670 | 83.668 | 349 | 56 | 1 | 1 | 348 | 1 | 349 | 0.0 | 633 |
| MS.gene048337.t1 | AT1G15850 | 72.143 | 140 | 37 | 1 | 1 | 138 | 1 | 140 | 5.39e-68 | 210 |
| MS.gene048337.t1 | AT3G19590 | 35.362 | 345 | 200 | 8 | 9 | 342 | 2 | 334 | 2.02e-64 | 208 |
| MS.gene048337.t1 | AT1G49910 | 33.724 | 341 | 203 | 8 | 13 | 342 | 5 | 333 | 3.75e-60 | 197 |
| MS.gene048337.t1 | AT1G69400 | 26.059 | 307 | 207 | 7 | 18 | 315 | 7 | 302 | 5.68e-29 | 114 |
| MS.gene048337.t1 | AT1G69400 | 25.197 | 254 | 166 | 7 | 18 | 258 | 7 | 249 | 1.09e-21 | 93.6 |
| MS.gene048337.t1 | AT1G11160 | 34.211 | 114 | 70 | 1 | 28 | 141 | 104 | 212 | 1.85e-13 | 72.0 |
| MS.gene048337.t1 | AT1G11160 | 24.583 | 240 | 160 | 8 | 68 | 299 | 12 | 238 | 8.00e-13 | 70.1 |
| MS.gene048337.t1 | AT1G11160 | 34.211 | 114 | 70 | 1 | 28 | 141 | 62 | 170 | 1.86e-13 | 72.0 |
| MS.gene048337.t1 | AT1G11160 | 34.211 | 114 | 70 | 1 | 28 | 141 | 104 | 212 | 1.91e-13 | 72.0 |
| MS.gene048337.t1 | AT1G11160 | 24.583 | 240 | 160 | 8 | 68 | 299 | 12 | 238 | 7.88e-13 | 70.1 |
| MS.gene048337.t1 | AT1G11160 | 34.211 | 114 | 70 | 1 | 28 | 141 | 104 | 212 | 1.99e-13 | 72.0 |
| MS.gene048337.t1 | AT1G11160 | 24.583 | 240 | 160 | 8 | 68 | 299 | 12 | 238 | 9.40e-13 | 70.1 |
| MS.gene048337.t1 | AT1G61210 | 34.211 | 114 | 70 | 1 | 28 | 141 | 104 | 212 | 3.23e-13 | 71.2 |
| MS.gene048337.t1 | AT4G15900 | 23.871 | 310 | 193 | 10 | 13 | 309 | 165 | 444 | 3.50e-13 | 70.9 |
| MS.gene048337.t1 | AT1G61210 | 34.211 | 114 | 70 | 1 | 28 | 141 | 104 | 212 | 5.10e-13 | 70.9 |
| MS.gene048337.t1 | AT1G61210 | 34.211 | 114 | 70 | 1 | 28 | 141 | 104 | 212 | 5.15e-13 | 70.9 |
| MS.gene048337.t1 | AT5G23430 | 33.628 | 113 | 70 | 1 | 29 | 141 | 106 | 213 | 3.09e-12 | 68.2 |
| MS.gene048337.t1 | AT5G23430 | 33.628 | 113 | 70 | 1 | 29 | 141 | 106 | 213 | 3.32e-12 | 68.2 |
| MS.gene048337.t1 | AT5G23430 | 33.628 | 113 | 70 | 1 | 29 | 141 | 106 | 213 | 3.32e-12 | 68.2 |
| MS.gene048337.t1 | AT5G67320 | 21.656 | 314 | 213 | 7 | 13 | 303 | 254 | 557 | 3.53e-12 | 67.8 |
| MS.gene048337.t1 | AT3G16650 | 22.876 | 306 | 200 | 9 | 13 | 309 | 159 | 437 | 4.07e-12 | 67.4 |
| MS.gene048337.t1 | AT5G08390 | 33.628 | 113 | 70 | 1 | 29 | 141 | 106 | 213 | 5.08e-12 | 67.8 |
| MS.gene048337.t1 | AT5G08390 | 24.583 | 240 | 160 | 8 | 68 | 299 | 13 | 239 | 4.83e-11 | 64.7 |
Find 82 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACACCTTGTCTGATATTTC+AGG | 0.213527 | 8.4:+34454095 | None:intergenic |
| TATTACCGGATGGAAATTTA+AGG | 0.288149 | 8.4:+34453397 | None:intergenic |
| GTCAACAGACCGAGTATAAA+AGG | 0.294586 | 8.4:-34454129 | MS.gene048337:intron |
| GCATACCGCGTAAGCATATA+TGG | 0.318779 | 8.4:+34453030 | None:intergenic |
| CCGATCAACATGTCTTCTTT+CGG | 0.324051 | 8.4:-34457347 | None:intergenic |
| GTCTATAGGTTTGCTATGAT+TGG | 0.329481 | 8.4:-34450187 | MS.gene048337:intron |
| ATTACCTCATAGGATTTGTT+TGG | 0.347710 | 8.4:+34457302 | None:intergenic |
| TGAAGTAGCGACAAGGAAAT+TGG | 0.350526 | 8.4:+34457155 | None:intergenic |
| GAACAGGAGACTGAGGTTAA+AGG | 0.352225 | 8.4:-34449871 | MS.gene048337:intron |
| ATTTCCTTGTCGCTACTTCA+TGG | 0.367802 | 8.4:-34457152 | MS.gene048337:CDS |
| ACTGAAAGAAAGACTTGAAA+TGG | 0.382134 | 8.4:+34457185 | None:intergenic |
| GCGACAAGGAAATTGGCTTT+AGG | 0.385636 | 8.4:+34457162 | None:intergenic |
| ATGTAGTTCTTTGCTGTAGT+TGG | 0.387737 | 8.4:+34450140 | None:intergenic |
| TTCGTAATAGGTTCGGTGTT+GGG | 0.411339 | 8.4:-34457046 | MS.gene048337:intron |
| GACATTGTATAACATCGATC+AGG | 0.430312 | 8.4:+34455077 | None:intergenic |
| ATTGAATGTACCACAAGGTA+TGG | 0.430485 | 8.4:+34453063 | None:intergenic |
| AAGCCGCGAGCTGGATCAAC+AGG | 0.435624 | 8.4:-34449847 | MS.gene048337:CDS |
| GCGTTAGGTATTGGGATACT+AGG | 0.449325 | 8.4:-34455130 | MS.gene048337:intron |
| TTCTGGAGGTTGTGACAAGC+AGG | 0.452789 | 8.4:-34456812 | MS.gene048337:CDS |
| TGAAGGTGTTGTGCTCTGCT+TGG | 0.460595 | 8.4:-34456858 | MS.gene048337:intron |
| AGAAAGTAATGAGATATATT+CGG | 0.462052 | 8.4:-34453426 | MS.gene048337:CDS |
| ATCATGCGAAATTGAAGCCT+TGG | 0.463596 | 8.4:+34456987 | None:intergenic |
| ATCCATGCAATCTCTTTGAT+AGG | 0.470416 | 8.4:+34456730 | None:intergenic |
| TGTGGTACATTCAATAATGA+TGG | 0.470459 | 8.4:-34453055 | MS.gene048337:CDS |
| AATTGAAGCCTTGGGTGTAC+TGG | 0.471129 | 8.4:+34456996 | None:intergenic |
| ACACCTTGTCTGATATTTCA+GGG | 0.476154 | 8.4:+34454096 | None:intergenic |
| CTTCAACTTGCAGAATCCTC+AGG | 0.486798 | 8.4:-34455003 | MS.gene048337:intron |
| AACGCATAACATTACCTCAT+AGG | 0.487242 | 8.4:+34457292 | None:intergenic |
| GGGAGTTGTTGAGTATGCAC+TGG | 0.487395 | 8.4:+34455098 | None:intergenic |
| TTTCCTTGTCGCTACTTCAT+GGG | 0.488482 | 8.4:-34457151 | MS.gene048337:CDS |
| TGCTCTGCTTGGAAAGATGA+TGG | 0.491720 | 8.4:-34456847 | MS.gene048337:CDS |
| TTTCGTAATAGGTTCGGTGT+TGG | 0.491966 | 8.4:-34457047 | MS.gene048337:intron |
| GTTAAAGGTAAGCCGCGAGC+TGG | 0.502221 | 8.4:-34449856 | MS.gene048337:CDS |
| CAAAGAGATTGCATGGATAC+CGG | 0.503787 | 8.4:-34456725 | MS.gene048337:CDS |
| CGCTACTTCATGGGACAACC+AGG | 0.504108 | 8.4:-34457142 | MS.gene048337:intron |
| AGAAAGACTTGAAATGGAAT+CGG | 0.504515 | 8.4:+34457191 | None:intergenic |
| TGCTTTGTTGTGCATCATCA+AGG | 0.506986 | 8.4:+34453474 | None:intergenic |
| CTCCTATCAAAGAGATTGCA+TGG | 0.517842 | 8.4:-34456732 | MS.gene048337:CDS |
| GGCTCAATAGAGGGAAGGGT+TGG | 0.523184 | 8.4:-34453506 | MS.gene048337:CDS |
| CTGTAGGTTGGCTCAATAGA+GGG | 0.523662 | 8.4:-34453515 | MS.gene048337:intron |
| CCTGTAGGTTGGCTCAATAG+AGG | 0.529343 | 8.4:-34453516 | MS.gene048337:intron |
| GAACTCCTTAAATTTCCATC+CGG | 0.534710 | 8.4:-34453402 | MS.gene048337:intron |
| TTTGCAACTGCTGGATCTGA+CGG | 0.543216 | 8.4:-34453257 | MS.gene048337:CDS |
| GTTTGCTATGATTGGAGCAA+AGG | 0.546194 | 8.4:-34450179 | MS.gene048337:CDS |
| GAGACATCCTCTGATGGTTG+TGG | 0.546885 | 8.4:-34455051 | MS.gene048337:CDS |
| AAATCCAAACAAATCCTATG+AGG | 0.547226 | 8.4:-34457306 | MS.gene048337:intron |
| TCATGCGAAATTGAAGCCTT+GGG | 0.556441 | 8.4:+34456988 | None:intergenic |
| TCATGCATAGCAACGGTCAC+GGG | 0.559382 | 8.4:+34456757 | None:intergenic |
| TTGAATGTACCACAAGGTAT+GGG | 0.565862 | 8.4:+34453064 | None:intergenic |
| ATCATGCATAGCAACGGTCA+CGG | 0.570899 | 8.4:+34456756 | None:intergenic |
| CGCCCCTGAAATATCAGACA+AGG | 0.571242 | 8.4:-34454099 | MS.gene048337:CDS |
| GAAGACAATCAGATTCCTAT+CGG | 0.589539 | 8.4:+34455022 | None:intergenic |
| GTGGCTAAAAGACTCATCTC+CGG | 0.590432 | 8.4:+34456706 | None:intergenic |
| CATATATATGCACTTACCAC+AGG | 0.593393 | 8.4:-34450120 | MS.gene048337:intron |
| AAGACTTGAAATGGAATCGG+TGG | 0.593663 | 8.4:+34457194 | None:intergenic |
| CTGATGTGAACAGGAGACTG+AGG | 0.607452 | 8.4:-34449878 | MS.gene048337:intron |
| GGTTGGCTCAATAGAGGGAA+GGG | 0.612759 | 8.4:-34453510 | MS.gene048337:CDS |
| AGACATCCTCTGATGGTTGT+GGG | 0.614239 | 8.4:-34455050 | MS.gene048337:CDS |
| AGGTTGGCTCAATAGAGGGA+AGG | 0.615075 | 8.4:-34453511 | MS.gene048337:CDS |
| TTGTTGAGTATGCACTGGGT+TGG | 0.621738 | 8.4:+34455103 | None:intergenic |
| GCGGTTCCCACAACCATCAG+AGG | 0.624752 | 8.4:+34455044 | None:intergenic |
| TGTTGTTACCAGTACACCCA+AGG | 0.625135 | 8.4:-34457004 | MS.gene048337:CDS |
| GGAGTTGTTGAGTATGCACT+GGG | 0.626401 | 8.4:+34455099 | None:intergenic |
| ACATTGTATAACATCGATCA+GGG | 0.631495 | 8.4:+34455078 | None:intergenic |
| CTTCCTGTTGATCCAGCTCG+CGG | 0.634887 | 8.4:+34449844 | None:intergenic |
| CTATGATTGGAGCAAAGGTG+CGG | 0.636918 | 8.4:-34450174 | MS.gene048337:CDS |
| GACAATCAGATTCCTATCGG+CGG | 0.637783 | 8.4:+34455025 | None:intergenic |
| TGGTTGTGGGAACCGCCGAT+AGG | 0.641214 | 8.4:-34455037 | MS.gene048337:CDS |
| GGACAGTAAACAGAGACTCA+AGG | 0.644768 | 8.4:-34453213 | MS.gene048337:intron |
| CACCTTGTCTGATATTTCAG+GGG | 0.645235 | 8.4:+34454097 | None:intergenic |
| AGACTTGAAATGGAATCGGT+GGG | 0.645745 | 8.4:+34457195 | None:intergenic |
| AGGAGCATCATGCATAGCAA+CGG | 0.647992 | 8.4:+34456750 | None:intergenic |
| ATATCTCATTACTTTCTCTG+TGG | 0.654168 | 8.4:+34453432 | None:intergenic |
| GGTTGCCCTCCAGACAAAAG+CGG | 0.656877 | 8.4:+34456778 | None:intergenic |
| TGTAGTCAGCCCATACCTTG+TGG | 0.658844 | 8.4:-34453073 | MS.gene048337:CDS |
| GTCAGTGAGACATCCTCTGA+TGG | 0.664484 | 8.4:-34455057 | MS.gene048337:CDS |
| GTGACAAGCAGGCTAAGATG+TGG | 0.674018 | 8.4:-34456801 | MS.gene048337:CDS |
| TGTTGGGAGATATCAAAGAA+CGG | 0.679052 | 8.4:-34457030 | MS.gene048337:CDS |
| TGTCCCATGAAGTAGCGACA+AGG | 0.689683 | 8.4:+34457148 | None:intergenic |
| CTTGAAATGGAATCGGTGGG+TGG | 0.706485 | 8.4:+34457198 | None:intergenic |
| TGGTTCCATATATGCTTACG+CGG | 0.709671 | 8.4:-34453035 | MS.gene048337:intron |
| TCATTATTGAATGTACCACA+AGG | 0.728862 | 8.4:+34453058 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 34449837 | 34457360 | 34449837 | ID=MS.gene048337 |
| chr8.4 | mRNA | 34449837 | 34457360 | 34449837 | ID=MS.gene048337.t1;Parent=MS.gene048337 |
| chr8.4 | exon | 34457307 | 34457360 | 34457307 | ID=MS.gene048337.t1.exon1;Parent=MS.gene048337.t1 |
| chr8.4 | CDS | 34457307 | 34457360 | 34457307 | ID=cds.MS.gene048337.t1;Parent=MS.gene048337.t1 |
| chr8.4 | exon | 34457143 | 34457229 | 34457143 | ID=MS.gene048337.t1.exon2;Parent=MS.gene048337.t1 |
| chr8.4 | CDS | 34457143 | 34457229 | 34457143 | ID=cds.MS.gene048337.t1;Parent=MS.gene048337.t1 |
| chr8.4 | exon | 34456981 | 34457058 | 34456981 | ID=MS.gene048337.t1.exon3;Parent=MS.gene048337.t1 |
| chr8.4 | CDS | 34456981 | 34457058 | 34456981 | ID=cds.MS.gene048337.t1;Parent=MS.gene048337.t1 |
| chr8.4 | exon | 34456682 | 34456875 | 34456682 | ID=MS.gene048337.t1.exon4;Parent=MS.gene048337.t1 |
| chr8.4 | CDS | 34456682 | 34456875 | 34456682 | ID=cds.MS.gene048337.t1;Parent=MS.gene048337.t1 |
| chr8.4 | exon | 34455004 | 34455145 | 34455004 | ID=MS.gene048337.t1.exon5;Parent=MS.gene048337.t1 |
| chr8.4 | CDS | 34455004 | 34455145 | 34455004 | ID=cds.MS.gene048337.t1;Parent=MS.gene048337.t1 |
| chr8.4 | exon | 34454063 | 34454143 | 34454063 | ID=MS.gene048337.t1.exon6;Parent=MS.gene048337.t1 |
| chr8.4 | CDS | 34454063 | 34454143 | 34454063 | ID=cds.MS.gene048337.t1;Parent=MS.gene048337.t1 |
| chr8.4 | exon | 34453403 | 34453531 | 34453403 | ID=MS.gene048337.t1.exon7;Parent=MS.gene048337.t1 |
| chr8.4 | CDS | 34453403 | 34453531 | 34453403 | ID=cds.MS.gene048337.t1;Parent=MS.gene048337.t1 |
| chr8.4 | exon | 34453214 | 34453291 | 34453214 | ID=MS.gene048337.t1.exon8;Parent=MS.gene048337.t1 |
| chr8.4 | CDS | 34453214 | 34453291 | 34453214 | ID=cds.MS.gene048337.t1;Parent=MS.gene048337.t1 |
| chr8.4 | exon | 34453036 | 34453107 | 34453036 | ID=MS.gene048337.t1.exon9;Parent=MS.gene048337.t1 |
| chr8.4 | CDS | 34453036 | 34453107 | 34453036 | ID=cds.MS.gene048337.t1;Parent=MS.gene048337.t1 |
| chr8.4 | exon | 34450121 | 34450201 | 34450121 | ID=MS.gene048337.t1.exon10;Parent=MS.gene048337.t1 |
| chr8.4 | CDS | 34450121 | 34450201 | 34450121 | ID=cds.MS.gene048337.t1;Parent=MS.gene048337.t1 |
| chr8.4 | exon | 34449837 | 34449887 | 34449837 | ID=MS.gene048337.t1.exon11;Parent=MS.gene048337.t1 |
| chr8.4 | CDS | 34449837 | 34449887 | 34449837 | ID=cds.MS.gene048337.t1;Parent=MS.gene048337.t1 |
| Gene Sequence |
| Protein sequence |
