AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene048867

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene048867.t1 MTR_8g098930 97.536 487 12 0 1 487 1 487 0.0 1000
MS.gene048867.t1 MTR_8g028600 64.792 480 158 3 10 486 9 480 0.0 669
MS.gene048867.t1 MTR_7g079080 65.377 491 158 4 1 486 1 484 0.0 669
MS.gene048867.t1 MTR_4g097540 55.011 449 194 3 10 450 2 450 0.0 542
MS.gene048867.t1 MTR_3g103550 53.571 448 199 4 11 451 1 446 0.0 527
MS.gene048867.t1 MTR_5g015020 54.503 433 191 4 10 436 2 434 0.0 525
MS.gene048867.t1 MTR_8g101750 56.132 424 177 2 12 435 12 426 0.0 518
MS.gene048867.t1 MTR_6g091760 55.581 430 186 3 12 441 14 438 0.0 514
MS.gene048867.t1 MTR_7g079080 63.636 385 129 3 107 486 1 379 1.99e-180 510
MS.gene048867.t1 MTR_8g101820 51.869 428 178 4 12 436 12 414 3.67e-164 471
MS.gene048867.t1 MTR_6g463260 41.882 425 243 2 12 435 104 525 2.25e-133 396
MS.gene048867.t1 MTR_5g011400 39.803 407 242 1 25 431 85 488 7.84e-117 353
MS.gene048867.t1 MTR_2g090730 47.312 93 45 1 65 157 12 100 7.39e-25 102
MS.gene048867.t1 MTR_8g023140 23.647 351 241 12 94 422 158 503 8.05e-18 86.7
MS.gene048867.t1 MTR_5g033230 24.781 343 233 13 87 408 100 438 2.63e-16 81.6
MS.gene048867.t1 MTR_5g033230 24.781 343 233 13 87 408 100 438 2.77e-16 81.6
MS.gene048867.t1 MTR_7g106830 21.972 355 240 14 95 436 135 465 5.41e-16 80.5
MS.gene048867.t1 MTR_4g011820 22.599 354 234 14 87 432 61 382 5.85e-15 77.0
MS.gene048867.t1 MTR_2g090615 23.947 380 219 17 89 438 77 416 1.26e-13 73.2
MS.gene048867.t1 MTR_1807s0010 27.041 196 127 6 95 289 69 249 3.63e-13 71.6
MS.gene048867.t1 MTR_8g021970 37.037 108 45 4 233 340 11 95 4.85e-13 65.5
MS.gene048867.t1 MTR_3g072670 22.613 398 248 15 45 422 85 442 5.02e-12 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene048867.t1 AT4G11280 65.784 491 145 4 11 487 14 495 0.0 656
MS.gene048867.t1 AT3G61510 61.856 485 171 3 10 485 9 488 0.0 642
MS.gene048867.t1 AT1G01480 59.839 498 185 6 1 485 1 496 0.0 638
MS.gene048867.t1 AT4G37770 58.275 429 172 3 11 435 3 428 0.0 549
MS.gene048867.t1 AT2G22810 55.349 430 190 2 12 439 4 433 0.0 516
MS.gene048867.t1 AT4G08040 53.846 429 196 2 11 439 1 427 0.0 514
MS.gene048867.t1 AT5G65800 54.128 436 197 2 12 445 4 438 1.21e-179 512
MS.gene048867.t1 AT3G49700 54.005 437 198 2 12 446 4 439 1.21e-179 512
MS.gene048867.t1 AT4G26200 55.765 425 186 2 12 435 18 441 6.18e-177 504
MS.gene048867.t1 AT1G01480 57.908 392 150 6 107 485 1 390 1.50e-167 478
MS.gene048867.t1 AT5G51690 42.579 411 232 2 25 434 82 489 1.01e-125 375
MS.gene048867.t1 AT1G62960 42.131 413 229 6 22 432 143 547 5.14e-122 368
MS.gene048867.t1 AT5G51690 43.539 356 198 1 79 434 14 366 2.06e-114 342
MS.gene048867.t1 AT2G22250 24.533 375 229 16 80 432 74 416 9.00e-20 92.0
MS.gene048867.t1 AT2G22250 24.533 375 229 16 80 432 121 463 1.57e-19 91.7
MS.gene048867.t1 AT2G22250 24.533 375 229 16 80 432 121 463 1.57e-19 91.7
MS.gene048867.t1 AT1G23310 25.000 348 226 15 87 408 100 438 1.69e-18 88.2
MS.gene048867.t1 AT1G17290 26.298 289 195 10 94 368 177 461 1.93e-18 88.6
MS.gene048867.t1 AT1G23310 25.000 348 226 15 87 408 100 438 2.62e-18 87.8
MS.gene048867.t1 AT1G70580 25.581 301 205 12 87 372 100 396 7.32e-18 86.7
MS.gene048867.t1 AT1G70580 25.581 301 205 12 87 372 100 396 7.32e-18 86.7
MS.gene048867.t1 AT1G70580 25.581 301 205 12 87 372 100 396 7.32e-18 86.7
MS.gene048867.t1 AT1G70580 25.581 301 205 12 87 372 100 396 7.32e-18 86.7
MS.gene048867.t1 AT1G72330 23.204 362 252 12 94 434 174 530 3.55e-17 84.7
MS.gene048867.t1 AT1G72330 22.400 375 252 12 94 434 174 543 1.64e-15 79.3
MS.gene048867.t1 AT5G36160 22.554 368 240 14 89 440 79 417 1.10e-14 76.3
MS.gene048867.t1 AT1G72330 26.809 235 163 7 94 323 174 404 3.14e-14 75.1
MS.gene048867.t1 AT1G77670 22.980 396 257 16 45 432 78 433 2.06e-12 69.3
MS.gene048867.t1 AT5G53970 22.025 395 260 16 68 440 40 408 8.30e-11 64.3

Find 92 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCTGATTTGGTTCAAAAT+TGG 0.164302 4.2:+5685150 MS.gene048867:CDS
CGTATATTTCATCGCTTATT+AGG 0.237518 4.2:-5686401 None:intergenic
AGAGTACAAATTGAGGCTTC+TGG 0.284872 4.2:-5685185 None:intergenic
TTTCCAGCTTACTGCTGATT+TGG 0.296356 4.2:+5685139 MS.gene048867:intron
TTTGCACTTTCGCAGATAAC+TGG 0.298899 4.2:-5686207 None:intergenic
AGAGATTTGAGGTGGAGAAC+AGG 0.302380 4.2:+5686171 MS.gene048867:CDS
GCGCGCAAAATGTCGAGTTT+TGG 0.341792 4.2:+5686603 MS.gene048867:CDS
AGCTACATTTGAATCAGAAT+TGG 0.358107 4.2:+5686830 MS.gene048867:CDS
ATTTGTGGTTCAGAATAATA+AGG 0.368943 4.2:+5686998 MS.gene048867:CDS
AGTACATAATTTCAAAGAAA+TGG 0.381772 4.2:+5685214 MS.gene048867:CDS
TCAGAATAATAAGGAGGTTA+TGG 0.384538 4.2:+5687007 MS.gene048867:CDS
AACACAGACACAATACTTGA+TGG 0.385707 4.2:+5686635 MS.gene048867:CDS
GCCTCAATTTGTACTCTAGA+AGG 0.386175 4.2:+5685191 MS.gene048867:CDS
ACAAGAGGTGACTGAGGAAA+TGG 0.387130 4.2:-5687125 None:intergenic
TCGTCATGGTGTTATCCAAA+TGG 0.389818 4.2:+5684985 MS.gene048867:CDS
TAAACAATTAATTCCAACTT+TGG 0.390119 4.2:-5686754 None:intergenic
TCATTTCATTGTTCTGAGCC+AGG 0.391963 4.2:+5686906 MS.gene048867:CDS
ACTAGTGGATTAACCAAAGT+TGG 0.392203 4.2:+5686741 MS.gene048867:CDS
GAGTACAAATTGAGGCTTCT+GGG 0.396801 4.2:-5685184 None:intergenic
GAAACATCATCTTACTTTGA+TGG 0.397210 4.2:+5684920 MS.gene048867:CDS
AATGGGTCTTGCAGAGAATC+AGG 0.399230 4.2:+5685003 MS.gene048867:CDS
GATTCTCTGCAAGACCCATT+TGG 0.400674 4.2:-5685000 None:intergenic
GTGCCTACTCCTTACTATCC+AGG 0.402112 4.2:+5685507 MS.gene048867:CDS
GGATTTGAGAGGACTTCTTA+AGG 0.406992 4.2:+5686806 MS.gene048867:CDS
TCAGATATGCTTATGAAACT+TGG 0.407331 4.2:-5686444 None:intergenic
TTGAATCAGAATTGGAACTT+TGG 0.414840 4.2:+5686838 MS.gene048867:CDS
CGGTCCATAACTGTGCCTAA+TGG 0.421374 4.2:-5686330 None:intergenic
CAACGGTTCTTAATGTGCTT+CGG 0.426255 4.2:-5686350 None:intergenic
GTCATGAGTGGTGGAGCAAC+TGG 0.428446 4.2:+5685432 MS.gene048867:CDS
AACTGTGCCTAATGGATTTG+AGG 0.440159 4.2:-5686322 None:intergenic
CTTGCTGGCATAGTAATTTG+AGG 0.441796 4.2:+5687051 MS.gene048867:CDS
ACAAAGGATTAGATCATTTG+TGG 0.445312 4.2:+5686983 MS.gene048867:CDS
ATTAATTGTTTACAAAGTAA+TGG 0.455659 4.2:+5686765 MS.gene048867:CDS
TGTACATACCCTGGATAGTA+AGG 0.457320 4.2:-5685516 None:intergenic
TTGAGGTTAAGCCTTAAAAC+AGG 0.470153 4.2:+5687068 MS.gene048867:CDS
AAATCCATTAGGCACAGTTA+TGG 0.479153 4.2:+5686326 MS.gene048867:CDS
TCAAAAGACATGGGATTCCC+TGG 0.480186 4.2:+5686531 MS.gene048867:CDS
ACTGTGCCTAATGGATTTGA+GGG 0.481893 4.2:-5686321 None:intergenic
CATCATCTTACTTTGATGGA+TGG 0.487153 4.2:+5684924 MS.gene048867:CDS
GATTCTGATGTTATCTTCTG+TGG 0.488021 4.2:-5686277 None:intergenic
AGAAGAACACTAAACCTTGC+TGG 0.489355 4.2:+5687036 MS.gene048867:CDS
CAGATATGCTTATGAAACTT+GGG 0.491656 4.2:-5686443 None:intergenic
GCAGATTTGATAGAGATTTG+AGG 0.492142 4.2:+5686160 MS.gene048867:intron
CATTTCATTGTTCTGAGCCA+GGG 0.492750 4.2:+5686907 MS.gene048867:CDS
ACCAGGATCTGCCAAACAAA+AGG 0.493747 4.2:-5685472 None:intergenic
CGTCATGGTGTTATCCAAAT+GGG 0.503114 4.2:+5684986 MS.gene048867:CDS
AAACTATGTGAACGAGGTTA+CGG 0.508352 4.2:-5686500 None:intergenic
TCTTAGACAACAATTGAGGT+TGG 0.527263 4.2:-5684874 None:intergenic
ACAAATCCCTCAAATCCATT+AGG 0.528896 4.2:+5686315 MS.gene048867:CDS
GCTATCTTAGACAACAATTG+AGG 0.540964 4.2:-5684878 None:intergenic
GAAGATAACATCAGAATCAA+AGG 0.542170 4.2:+5686282 MS.gene048867:CDS
TCTAAGATAGCCACTGGTGA+TGG 0.550202 4.2:+5684890 MS.gene048867:CDS
CCACTCACATTTCTGAACTC+TGG 0.552742 4.2:-5685263 None:intergenic
TGCCTACTCCTTACTATCCA+GGG 0.556150 4.2:+5685508 MS.gene048867:CDS
TCCTTCTAGAGTACAAATTG+AGG 0.558961 4.2:-5685192 None:intergenic
CAAGTAGTGGCTTTAACAAG+AGG 0.562133 4.2:-5687140 None:intergenic
ATGTGCAAATTGCTTTACAA+AGG 0.563606 4.2:+5686967 MS.gene048867:CDS
GATTTGATAGAGATTTGAGG+TGG 0.566695 4.2:+5686163 MS.gene048867:intron
CCACTCATGACAATACGATC+AGG 0.570761 4.2:-5685420 None:intergenic
ATCTGCCAAACAAAAGGCAG+TGG 0.571931 4.2:-5685466 None:intergenic
CTCAAATTACTATGCCAGCA+AGG 0.576441 4.2:-5687050 None:intergenic
TTGTTGTCTAAGATAGCCAC+TGG 0.576629 4.2:+5684884 MS.gene048867:CDS
TGGAGCAACTGGAGCACATG+AGG 0.576944 4.2:+5685443 MS.gene048867:CDS
GGTTAAGCCTTAAAACAGGA+AGG 0.579184 4.2:+5687072 MS.gene048867:CDS
GTTAAGATTAATGTTTCACC+TGG 0.580822 4.2:+5686879 MS.gene048867:CDS
AATTGTTTACAAAGTAATGG+TGG 0.584470 4.2:+5686768 MS.gene048867:CDS
TTATACCAACTCTAAAACCA+GGG 0.584528 4.2:-5686548 None:intergenic
TACAGTCTTTCAAAAGACAT+GGG 0.586545 4.2:+5686522 MS.gene048867:CDS
GACTGAGGAAATGGAGAATG+AGG 0.586929 4.2:-5687116 None:intergenic
AAATTCATGTCTAGAACAAG+AGG 0.590021 4.2:+5685378 MS.gene048867:CDS
ATGCTAAATAATTCAAGTAG+TGG 0.595412 4.2:-5687153 None:intergenic
TTACAGTCTTTCAAAAGACA+TGG 0.595760 4.2:+5686521 MS.gene048867:CDS
TTCATTGATGAAATTTACAA+CGG 0.599733 4.2:-5686367 None:intergenic
TAGAGTGTGTTATGCTAACA+TGG 0.616578 4.2:+5686935 MS.gene048867:CDS
ATTATACCAACTCTAAAACC+AGG 0.620538 4.2:-5686549 None:intergenic
TTCTTGCTGAAAGTGCAAAG+AGG 0.621704 4.2:+5686694 MS.gene048867:CDS
TTCATTGTTCTGAGCCAGGG+TGG 0.625682 4.2:+5686910 MS.gene048867:CDS
GATCGTATTGTCATGAGTGG+TGG 0.626364 4.2:+5685423 MS.gene048867:CDS
CGATGAAATATACGCTGCAA+CGG 0.630366 4.2:+5686410 MS.gene048867:CDS
TGTGGTTCAGAATAATAAGG+AGG 0.634977 4.2:+5687001 MS.gene048867:CDS
GTGCAAAGAGGTTAGCACAA+AGG 0.640437 4.2:+5686706 MS.gene048867:CDS
TACCCTGGATAGTAAGGAGT+AGG 0.653482 4.2:-5685510 None:intergenic
CCTGATCGTATTGTCATGAG+TGG 0.663504 4.2:+5685420 MS.gene048867:CDS
GAACAATGAAATGAAACACC+AGG 0.667249 4.2:-5686897 None:intergenic
ATATGCTTATGAAACTTGGG+TGG 0.669331 4.2:-5686440 None:intergenic
TTCACCATGTCCATCACCAG+TGG 0.671313 4.2:-5684900 None:intergenic
GACTGTAAACTATGTGAACG+AGG 0.673179 4.2:-5686506 None:intergenic
TTTGTGTGGATGGATTTGAG+AGG 0.680530 4.2:+5686795 MS.gene048867:CDS
TTTGCGCGCACAATTTACAA+CGG 0.684836 4.2:-5686589 None:intergenic
ATAGCCACTGGTGATGGACA+TGG 0.685257 4.2:+5684896 MS.gene048867:CDS
GCTTTAACAAGAGGTGACTG+AGG 0.694413 4.2:-5687131 None:intergenic
TAACACACTCTAAACCACCC+TGG 0.698750 4.2:-5686924 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTAATATGAATTTTTAC+AGG + chr4.2:5685347-5685366 MS.gene048867:intron 10.0%
!! AAAAATAAAGATGAAGAAAA+GGG - chr4.2:5685559-5685578 None:intergenic 15.0%
!! ATTAATTGTTTACAAAGTAA+TGG + chr4.2:5686765-5686784 MS.gene048867:CDS 15.0%
!! GAATATTAAAGTAATAACTA+AGG - chr4.2:5685857-5685876 None:intergenic 15.0%
!! AAAATAAAGATGAAGAAAAG+GGG - chr4.2:5685558-5685577 None:intergenic 20.0%
!! AAAATAAATAATTGCTCTGA+TGG - chr4.2:5686001-5686020 None:intergenic 20.0%
!! AGTACATAATTTCAAAGAAA+TGG + chr4.2:5685214-5685233 MS.gene048867:CDS 20.0%
!! GAAAAATAAAGATGAAGAAA+AGG - chr4.2:5685560-5685579 None:intergenic 20.0%
!! TAAACAATTAATTCCAACTT+TGG - chr4.2:5686757-5686776 None:intergenic 20.0%
!! TAGAATATTATATGACTATG+TGG - chr4.2:5686091-5686110 None:intergenic 20.0%
!! TTCATTGATGAAATTTACAA+CGG - chr4.2:5686370-5686389 None:intergenic 20.0%
!!! AATGTTTTTGTTTTACTTTC+TGG - chr4.2:5686054-5686073 None:intergenic 20.0%
! AATTGTTTACAAAGTAATGG+TGG + chr4.2:5686768-5686787 MS.gene048867:CDS 25.0%
! ATTTGTGGTTCAGAATAATA+AGG + chr4.2:5686998-5687017 MS.gene048867:CDS 25.0%
! TAAAAATATACTGCATGGAT+AGG - chr4.2:5685089-5685108 None:intergenic 25.0%
!! TCAAGTTTAAGATATGATGA+TGG - chr4.2:5685636-5685655 None:intergenic 25.0%
!! TTTTTCATATGCATCTTCTA+AGG - chr4.2:5686256-5686275 None:intergenic 25.0%
!!! TTACTTTCTGGTTTGTATAT+AGG - chr4.2:5686042-5686061 None:intergenic 25.0%
ACAAAGGATTAGATCATTTG+TGG + chr4.2:5686983-5687002 MS.gene048867:CDS 30.0%
AGAGCTAAAAATATACTGCA+TGG - chr4.2:5685094-5685113 None:intergenic 30.0%
AGCTACATTTGAATCAGAAT+TGG + chr4.2:5686830-5686849 MS.gene048867:CDS 30.0%
ATGTGCAAATTGCTTTACAA+AGG + chr4.2:5686967-5686986 MS.gene048867:CDS 30.0%
CAGATATGCTTATGAAACTT+GGG - chr4.2:5686446-5686465 None:intergenic 30.0%
CGTATATTTCATCGCTTATT+AGG - chr4.2:5686404-5686423 None:intergenic 30.0%
GAAACATCATCTTACTTTGA+TGG + chr4.2:5684920-5684939 MS.gene048867:CDS 30.0%
GAAGATAACATCAGAATCAA+AGG + chr4.2:5686282-5686301 MS.gene048867:CDS 30.0%
GTTAAGATTAATGTTTCACC+TGG + chr4.2:5686879-5686898 MS.gene048867:CDS 30.0%
TAATAACTAAGGCTGATTAG+TGG - chr4.2:5685846-5685865 None:intergenic 30.0%
TACAGTCTTTCAAAAGACAT+GGG + chr4.2:5686522-5686541 MS.gene048867:CDS 30.0%
TCAGAATAATAAGGAGGTTA+TGG + chr4.2:5687007-5687026 MS.gene048867:CDS 30.0%
TCAGATATGCTTATGAAACT+TGG - chr4.2:5686447-5686466 None:intergenic 30.0%
TTACAGTCTTTCAAAAGACA+TGG + chr4.2:5686521-5686540 MS.gene048867:CDS 30.0%
TTGAATCAGAATTGGAACTT+TGG + chr4.2:5686838-5686857 MS.gene048867:CDS 30.0%
! AAATTCATGTCTAGAACAAG+AGG + chr4.2:5685378-5685397 MS.gene048867:CDS 30.0%
! ATATGAATTTTTACAGGCTG+TGG + chr4.2:5685353-5685372 MS.gene048867:intron 30.0%
! CAAAGAAATGGCTAATTTTC+AGG + chr4.2:5685226-5685245 MS.gene048867:CDS 30.0%
!! ATTATACCAACTCTAAAACC+AGG - chr4.2:5686552-5686571 None:intergenic 30.0%
!! GCTAATTTTCAGGATTATCA+TGG + chr4.2:5685236-5685255 MS.gene048867:CDS 30.0%
!! TTATACCAACTCTAAAACCA+GGG - chr4.2:5686551-5686570 None:intergenic 30.0%
!!! GATTTTTGGTTGGATGAAAA+GGG - chr4.2:5684967-5684986 None:intergenic 30.0%
AAACTATGTGAACGAGGTTA+CGG - chr4.2:5686503-5686522 None:intergenic 35.0%
AAATCCATTAGGCACAGTTA+TGG + chr4.2:5686326-5686345 MS.gene048867:CDS 35.0%
AACACAGACACAATACTTGA+TGG + chr4.2:5686635-5686654 MS.gene048867:CDS 35.0%
ACAAATCCCTCAAATCCATT+AGG + chr4.2:5686315-5686334 MS.gene048867:CDS 35.0%
ATATGCTTATGAAACTTGGG+TGG - chr4.2:5686443-5686462 None:intergenic 35.0%
CAGGTATATATCAAGCTTTG+AGG + chr4.2:5685022-5685041 MS.gene048867:intron 35.0%
CATCATCTTACTTTGATGGA+TGG + chr4.2:5684924-5684943 MS.gene048867:CDS 35.0%
GAACAATGAAATGAAACACC+AGG - chr4.2:5686900-5686919 None:intergenic 35.0%
GATTCTGATGTTATCTTCTG+TGG - chr4.2:5686280-5686299 None:intergenic 35.0%
GATTTGATAGAGATTTGAGG+TGG + chr4.2:5686163-5686182 MS.gene048867:intron 35.0%
GCAGATTTGATAGAGATTTG+AGG + chr4.2:5686160-5686179 MS.gene048867:intron 35.0%
GCTATCTTAGACAACAATTG+AGG - chr4.2:5684881-5684900 None:intergenic 35.0%
TAGAGTGTGTTATGCTAACA+TGG + chr4.2:5686935-5686954 MS.gene048867:CDS 35.0%
TCACTGCACTGTAAAGTAAA+CGG + chr4.2:5685751-5685770 MS.gene048867:intron 35.0%
TCTTAGACAACAATTGAGGT+TGG - chr4.2:5684877-5684896 None:intergenic 35.0%
TGAATTGAATGTACATACCC+TGG - chr4.2:5685528-5685547 None:intergenic 35.0%
TGTGGTTCAGAATAATAAGG+AGG + chr4.2:5687001-5687020 MS.gene048867:CDS 35.0%
TTGAGGTTAAGCCTTAAAAC+AGG + chr4.2:5687068-5687087 MS.gene048867:CDS 35.0%
TTTATAGTGCAATCAGCTAC+AGG + chr4.2:5685672-5685691 MS.gene048867:intron 35.0%
! AGGTACAGAATTTTCACTAG+TGG + chr4.2:5686726-5686745 MS.gene048867:CDS 35.0%
! CATCAAACCTTCCTGTTTTA+AGG - chr4.2:5687082-5687101 None:intergenic 35.0%
! GATAACACCATGACGATTTT+TGG - chr4.2:5684981-5685000 None:intergenic 35.0%
!! ACTAGTGGATTAACCAAAGT+TGG + chr4.2:5686741-5686760 MS.gene048867:CDS 35.0%
!! AGGTAAAACATTTTCAAGCG+CGG + chr4.2:5685775-5685794 MS.gene048867:intron 35.0%
!! CTGCTGATTTGGTTCAAAAT+TGG + chr4.2:5685150-5685169 MS.gene048867:CDS 35.0%
!! TCCTTCTAGAGTACAAATTG+AGG - chr4.2:5685195-5685214 None:intergenic 35.0%
!!! CGATTTTTGGTTGGATGAAA+AGG - chr4.2:5684968-5684987 None:intergenic 35.0%
!!! GGAACTGACTTTTTCAACAA+GGG + chr4.2:5685913-5685932 MS.gene048867:intron 35.0%
!!! TGGAACTGACTTTTTCAACA+AGG + chr4.2:5685912-5685931 MS.gene048867:intron 35.0%
AGAAGAACACTAAACCTTGC+TGG + chr4.2:5687036-5687055 MS.gene048867:CDS 40.0%
AGAGTACAAATTGAGGCTTC+TGG - chr4.2:5685188-5685207 None:intergenic 40.0%
CAACGGTTCTTAATGTGCTT+CGG - chr4.2:5686353-5686372 None:intergenic 40.0%
CATCCAACCAAAAATCGTCA+TGG + chr4.2:5684971-5684990 MS.gene048867:CDS 40.0%
CGATGAAATATACGCTGCAA+CGG + chr4.2:5686410-5686429 MS.gene048867:CDS 40.0%
CTCAAATTACTATGCCAGCA+AGG - chr4.2:5687053-5687072 None:intergenic 40.0%
CTTGCTGGCATAGTAATTTG+AGG + chr4.2:5687051-5687070 MS.gene048867:CDS 40.0%
GACTGTAAACTATGTGAACG+AGG - chr4.2:5686509-5686528 None:intergenic 40.0%
GAGTACAAATTGAGGCTTCT+GGG - chr4.2:5685187-5685206 None:intergenic 40.0%
GCCTCAATTTGTACTCTAGA+AGG + chr4.2:5685191-5685210 MS.gene048867:CDS 40.0%
GGATTTGAGAGGACTTCTTA+AGG + chr4.2:5686806-5686825 MS.gene048867:CDS 40.0%
GGTTAAGCCTTAAAACAGGA+AGG + chr4.2:5687072-5687091 MS.gene048867:CDS 40.0%
TCCATCAAAATTCACTACCC+TGG - chr4.2:5685896-5685915 None:intergenic 40.0%
TGTACATACCCTGGATAGTA+AGG - chr4.2:5685519-5685538 None:intergenic 40.0%
TTTGCACTTTCGCAGATAAC+TGG - chr4.2:5686210-5686229 None:intergenic 40.0%
TTTGCGCGCACAATTTACAA+CGG - chr4.2:5686592-5686611 None:intergenic 40.0%
! CATTTCATTGTTCTGAGCCA+GGG + chr4.2:5686907-5686926 MS.gene048867:CDS 40.0%
! TCATTTCATTGTTCTGAGCC+AGG + chr4.2:5686906-5686925 MS.gene048867:CDS 40.0%
! TTCTTGCTGAAAGTGCAAAG+AGG + chr4.2:5686694-5686713 MS.gene048867:CDS 40.0%
! TTGTTGTCTAAGATAGCCAC+TGG + chr4.2:5684884-5684903 MS.gene048867:CDS 40.0%
! TTTCCAGCTTACTGCTGATT+TGG + chr4.2:5685139-5685158 MS.gene048867:intron 40.0%
! TTTGTGTGGATGGATTTGAG+AGG + chr4.2:5686795-5686814 MS.gene048867:CDS 40.0%
!! AACTGTGCCTAATGGATTTG+AGG - chr4.2:5686325-5686344 None:intergenic 40.0%
!! ACTGTGCCTAATGGATTTGA+GGG - chr4.2:5686324-5686343 None:intergenic 40.0%
!! CAAGTAGTGGCTTTAACAAG+AGG - chr4.2:5687143-5687162 None:intergenic 40.0%
!! CGTCATGGTGTTATCCAAAT+GGG + chr4.2:5684986-5685005 MS.gene048867:CDS 40.0%
!! TCGTCATGGTGTTATCCAAA+TGG + chr4.2:5684985-5685004 MS.gene048867:CDS 40.0%
!!! ACACCATGACGATTTTTGGT+TGG - chr4.2:5684977-5684996 None:intergenic 40.0%
!!! AGATCCTGGTGATGCTTTTT+TGG + chr4.2:5685485-5685504 MS.gene048867:CDS 40.0%
!!! GTAATGGTGGACTTTTTGTG+TGG + chr4.2:5686781-5686800 MS.gene048867:CDS 40.0%
AATGGGTCTTGCAGAGAATC+AGG + chr4.2:5685003-5685022 MS.gene048867:CDS 45.0%
ACAAGAGGTGACTGAGGAAA+TGG - chr4.2:5687128-5687147 None:intergenic 45.0%
ACCAGGATCTGCCAAACAAA+AGG - chr4.2:5685475-5685494 None:intergenic 45.0%
ATCTGCCAAACAAAAGGCAG+TGG - chr4.2:5685469-5685488 None:intergenic 45.0%
CCACTCACATTTCTGAACTC+TGG - chr4.2:5685266-5685285 None:intergenic 45.0%
CCACTCATGACAATACGATC+AGG - chr4.2:5685423-5685442 None:intergenic 45.0%
CCAGAGTTCAGAAATGTGAG+TGG + chr4.2:5685263-5685282 MS.gene048867:intron 45.0%
CCAGTCATCATTAGAAGCCA+GGG + chr4.2:5685876-5685895 MS.gene048867:intron 45.0%
CCTGATCGTATTGTCATGAG+TGG + chr4.2:5685420-5685439 MS.gene048867:CDS 45.0%
GAACCAAATCAGCAGTAAGC+TGG - chr4.2:5685145-5685164 None:intergenic 45.0%
GACTGAGGAAATGGAGAATG+AGG - chr4.2:5687119-5687138 None:intergenic 45.0%
GATCGTATTGTCATGAGTGG+TGG + chr4.2:5685423-5685442 MS.gene048867:CDS 45.0%
GATTCTCTGCAAGACCCATT+TGG - chr4.2:5685003-5685022 None:intergenic 45.0%
GCTTTAACAAGAGGTGACTG+AGG - chr4.2:5687134-5687153 None:intergenic 45.0%
GTGCAAAGAGGTTAGCACAA+AGG + chr4.2:5686706-5686725 MS.gene048867:CDS 45.0%
TAACACACTCTAAACCACCC+TGG - chr4.2:5686927-5686946 None:intergenic 45.0%
TACCCTGGATAGTAAGGAGT+AGG - chr4.2:5685513-5685532 None:intergenic 45.0%
TCAAAAGACATGGGATTCCC+TGG + chr4.2:5686531-5686550 MS.gene048867:CDS 45.0%
TCCAGTCATCATTAGAAGCC+AGG + chr4.2:5685875-5685894 MS.gene048867:intron 45.0%
TGCACTGTAAAGTAAACGGC+AGG + chr4.2:5685755-5685774 MS.gene048867:intron 45.0%
TGCCTACTCCTTACTATCCA+GGG + chr4.2:5685508-5685527 MS.gene048867:CDS 45.0%
! AGAGATTTGAGGTGGAGAAC+AGG + chr4.2:5686171-5686190 MS.gene048867:CDS 45.0%
! GCCAGGGTAGTGAATTTTGA+TGG + chr4.2:5685892-5685911 MS.gene048867:intron 45.0%
! TCTAAGATAGCCACTGGTGA+TGG + chr4.2:5684890-5684909 MS.gene048867:CDS 45.0%
!!! GGATTCCCTGGTTTTAGAGT+TGG + chr4.2:5686543-5686562 MS.gene048867:CDS 45.0%
!!! TGGTGGACTTTTTGTGTGGA+TGG + chr4.2:5686785-5686804 MS.gene048867:CDS 45.0%
!!! TAATTGATAATATTTATAAT+TGG - chr4.2:5685582-5685601 None:intergenic 5.0%
CCCTGGCTTCTAATGATGAC+TGG - chr4.2:5685879-5685898 None:intergenic 50.0%
CGGTCCATAACTGTGCCTAA+TGG - chr4.2:5686333-5686352 None:intergenic 50.0%
GTGCCTACTCCTTACTATCC+AGG + chr4.2:5685507-5685526 MS.gene048867:CDS 50.0%
TTCACCATGTCCATCACCAG+TGG - chr4.2:5684903-5684922 None:intergenic 50.0%
! GCGCGCAAAATGTCGAGTTT+TGG + chr4.2:5686603-5686622 MS.gene048867:CDS 50.0%
! TGAGGCCACTGCCTTTTGTT+TGG + chr4.2:5685461-5685480 MS.gene048867:CDS 50.0%
! TTCATTGTTCTGAGCCAGGG+TGG + chr4.2:5686910-5686929 MS.gene048867:CDS 50.0%
!! ATAGCCACTGGTGATGGACA+TGG + chr4.2:5684896-5684915 MS.gene048867:CDS 50.0%
!! GCCTTTTGTTTGGCAGATCC+TGG + chr4.2:5685471-5685490 MS.gene048867:CDS 50.0%
!! GGCACCAAAAAAGCATCACC+AGG - chr4.2:5685492-5685511 None:intergenic 50.0%
CAGTGCAGTGAGCTGCAAGT+CGG - chr4.2:5685742-5685761 None:intergenic 55.0%
TGGAGCAACTGGAGCACATG+AGG + chr4.2:5685443-5685462 MS.gene048867:CDS 55.0%
! GTCATGAGTGGTGGAGCAAC+TGG + chr4.2:5685432-5685451 MS.gene048867:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 5684854 5687163 5684854 ID=MS.gene048867
chr4.2 mRNA 5684854 5687163 5684854 ID=MS.gene048867.t1;Parent=MS.gene048867
chr4.2 exon 5684854 5685024 5684854 ID=MS.gene048867.t1.exon1;Parent=MS.gene048867.t1
chr4.2 CDS 5684854 5685024 5684854 ID=cds.MS.gene048867.t1;Parent=MS.gene048867.t1
chr4.2 exon 5685146 5685277 5685146 ID=MS.gene048867.t1.exon2;Parent=MS.gene048867.t1
chr4.2 CDS 5685146 5685277 5685146 ID=cds.MS.gene048867.t1;Parent=MS.gene048867.t1
chr4.2 exon 5685369 5685529 5685369 ID=MS.gene048867.t1.exon3;Parent=MS.gene048867.t1
chr4.2 CDS 5685369 5685529 5685369 ID=cds.MS.gene048867.t1;Parent=MS.gene048867.t1
chr4.2 exon 5686164 5687163 5686164 ID=MS.gene048867.t1.exon4;Parent=MS.gene048867.t1
chr4.2 CDS 5686164 5687163 5686164 ID=cds.MS.gene048867.t1;Parent=MS.gene048867.t1
Gene Sequence

>MS.gene048867

ATGGGACTTATGAGCATGAACCAACCTCAATTGTTGTCTAAGATAGCCACTGGTGATGGACATGGTGAAACATCATCTTACTTTGATGGATGGAAAGCTTATGATAAAGACCCTTTTCATCCAACCAAAAATCGTCATGGTGTTATCCAAATGGGTCTTGCAGAGAATCAGGTATATATCAAGCTTTGAGGTTTCATGTTCAAACTTTAAGAACTTGCATAAGTATTATTTTCCTATCCATGCAGTATATTTTTAGCTCTAAATTCTAATTCAATTATTTTTATATTTCCAGCTTACTGCTGATTTGGTTCAAAATTGGATAATGAGTAACCCAGAAGCCTCAATTTGTACTCTAGAAGGAGTACATAATTTCAAAGAAATGGCTAATTTTCAGGATTATCATGGTCTACCAGAGTTCAGAAATGTGAGTGGTGATAACAGTACATACAATAAGAATTTTGTATGAAATTTGATCTATATATAAACTAATATAAATTTAATATGAATTTTTACAGGCTGTGGCTAAATTCATGTCTAGAACAAGAGGAAATAGAGTGACGTTTAATCCTGATCGTATTGTCATGAGTGGTGGAGCAACTGGAGCACATGAGGCCACTGCCTTTTGTTTGGCAGATCCTGGTGATGCTTTTTTGGTGCCTACTCCTTACTATCCAGGGTATGTACATTCAATTCAATTTCAACCCCTTTTCTTCATCTTTATTTTTCCAATTATAAATATTATCAATTATTATTTTAATTTAGAAGATAGTGAAATGTAGCCATCATCATATCTTAAACTTGAATTATCTGTAGTTTGTTTTATAGTGCAATCAGCTACAGGTCATTAGATTAAGAGCGATCAAATGTATATCTTTCTTGATCTAACCGACTTGCAGCTCACTGCACTGTAAAGTAAACGGCAGGTAAAACATTTTCAAGCGCGGCATCATGTTCGTACGCATGTTCTTCAATTAAAATAGAATTAATCTCCACTAATCAGCCTTAGTTATTACTTTAATATTCCAGTCATCATTAGAAGCCAGGGTAGTGAATTTTGATGGAACTGACTTTTTCAACAAGGGAATAATTTTACAACATTTCAATTTCAACTGTGTTTGCAACAGTCAACTATCATATTCAAAAGCCATCAGAGCAATTATTTATTTTTGTTGACTTCGTATTCATCCTATATACAAACCAGAAAGTAAAACAAAAACATTTGTGCTTAAACTAACCACATAGTCATATAATATTCTAATATATCTACATGTTAATTATTTTCTAATGAGATGTTTGATTTCTTAATGCAGATTTGATAGAGATTTGAGGTGGAGAACAGGAGTTAAACTTGTTCCAGTTATCTGCGAAAGTGCAAACAATTTCAAATTAACAAAACAAGCCTTAGAAGATGCATATGAAAAAGCCACAGAAGATAACATCAGAATCAAAGGTTTACTCATAACAAATCCCTCAAATCCATTAGGCACAGTTATGGACCGAAGCACATTAAGAACCGTTGTAAATTTCATCAATGAAAAGCGTATTCACCTAATAAGCGATGAAATATACGCTGCAACGGTTTTTAGCCACCCAAGTTTCATAAGCATATCTGAAATATTAGAACATGACACAGACATTGAATGTGACCGTAACCTCGTTCACATAGTTTACAGTCTTTCAAAAGACATGGGATTCCCTGGTTTTAGAGTTGGTATAATATACTCTTACAATGATACCGTTGTAAATTGTGCGCGCAAAATGTCGAGTTTTGGATTAGTTTCAACACAGACACAATACTTGATGGCAAAAATGCTATCTGATGATGAATTTGTTGAAAAGTTTCTTGCTGAAAGTGCAAAGAGGTTAGCACAAAGGTACAGAATTTTCACTAGTGGATTAACCAAAGTTGGAATTAATTGTTTACAAAGTAATGGTGGACTTTTTGTGTGGATGGATTTGAGAGGACTTCTTAAGGAAGCTACATTTGAATCAGAATTGGAACTTTGGAGAGTGATTATTCATGAAGTTAAGATTAATGTTTCACCTGGTGTTTCATTTCATTGTTCTGAGCCAGGGTGGTTTAGAGTGTGTTATGCTAACATGGATGATAGAGATGTGCAAATTGCTTTACAAAGGATTAGATCATTTGTGGTTCAGAATAATAAGGAGGTTATGGTGTCTGAGAAGAACACTAAACCTTGCTGGCATAGTAATTTGAGGTTAAGCCTTAAAACAGGAAGGTTTGATGATATTGTAATGTCACCTCATTCTCCATTTCCTCAGTCACCTCTTGTTAAAGCCACTACTTGA
Protein sequence

>MS.gene048867.t1

MGLMSMNQPQLLSKIATGDGHGETSSYFDGWKAYDKDPFHPTKNRHGVIQMGLAENQLTADLVQNWIMSNPEASICTLEGVHNFKEMANFQDYHGLPEFRNAVAKFMSRTRGNRVTFNPDRIVMSGGATGAHEATAFCLADPGDAFLVPTPYYPGFDRDLRWRTGVKLVPVICESANNFKLTKQALEDAYEKATEDNIRIKGLLITNPSNPLGTVMDRSTLRTVVNFINEKRIHLISDEIYAATVFSHPSFISISEILEHDTDIECDRNLVHIVYSLSKDMGFPGFRVGIIYSYNDTVVNCARKMSSFGLVSTQTQYLMAKMLSDDEFVEKFLAESAKRLAQRYRIFTSGLTKVGINCLQSNGGLFVWMDLRGLLKEATFESELELWRVIIHEVKINVSPGVSFHCSEPGWFRVCYANMDDRDVQIALQRIRSFVVQNNKEVMVSEKNTKPCWHSNLRLSLKTGRFDDIVMSPHSPFPQSPLVKATT