Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90334.t1 | XP_003623021.2 | 74.8 | 416 | 80 | 8 | 1 | 405 | 40 | 441 | 8.30E-159 | 570.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90334.t1 | Q9SD53 | 24.3 | 423 | 267 | 17 | 3 | 400 | 61 | 455 | 4.3e-15 | 84.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90334.t1 | G7L3D3 | 74.8 | 416 | 80 | 8 | 1 | 405 | 40 | 441 | 6.0e-159 | 570.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049194 | MS.gene90334 | 0.821141 | 4.73E-53 | -1.69E-46 |
| MS.gene05143 | MS.gene90334 | 0.814345 | 1.62E-51 | -1.69E-46 |
| MS.gene052142 | MS.gene90334 | 0.831651 | 1.48E-55 | -1.69E-46 |
| MS.gene052187 | MS.gene90334 | 0.86711 | 1.74E-65 | -1.69E-46 |
| MS.gene052456 | MS.gene90334 | 0.81785 | 2.66E-52 | -1.69E-46 |
| MS.gene053074 | MS.gene90334 | 0.8218 | 3.33E-53 | -1.69E-46 |
| MS.gene053383 | MS.gene90334 | 0.841336 | 5.05E-58 | -1.69E-46 |
| MS.gene053798 | MS.gene90334 | 0.81434 | 1.62E-51 | -1.69E-46 |
| MS.gene055359 | MS.gene90334 | 0.882286 | 1.18E-70 | -1.69E-46 |
| MS.gene058168 | MS.gene90334 | 0.816764 | 4.67E-52 | -1.69E-46 |
| MS.gene059006 | MS.gene90334 | 0.819493 | 1.13E-52 | -1.69E-46 |
| MS.gene059632 | MS.gene90334 | 0.839997 | 1.13E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90334.t1 | MTR_7g060220 | 74.760 | 416 | 80 | 8 | 1 | 405 | 40 | 441 | 0.0 | 560 |
| MS.gene90334.t1 | MTR_7g060310 | 64.222 | 450 | 113 | 6 | 3 | 405 | 44 | 492 | 0.0 | 542 |
| MS.gene90334.t1 | MTR_0182s0060 | 60.780 | 487 | 103 | 9 | 1 | 405 | 41 | 521 | 0.0 | 535 |
| MS.gene90334.t1 | MTR_7g059810 | 71.292 | 418 | 94 | 7 | 3 | 405 | 43 | 449 | 0.0 | 533 |
| MS.gene90334.t1 | MTR_7g060320 | 68.925 | 428 | 103 | 7 | 3 | 403 | 43 | 467 | 0.0 | 526 |
| MS.gene90334.t1 | MTR_7g060115 | 63.507 | 422 | 124 | 8 | 3 | 405 | 42 | 452 | 3.96e-177 | 501 |
| MS.gene90334.t1 | MTR_7g060370 | 62.727 | 440 | 122 | 7 | 1 | 404 | 41 | 474 | 5.37e-172 | 489 |
| MS.gene90334.t1 | MTR_7g059540 | 60.465 | 430 | 137 | 8 | 3 | 403 | 44 | 469 | 5.88e-171 | 486 |
| MS.gene90334.t1 | MTR_7g059540 | 59.318 | 440 | 136 | 8 | 3 | 403 | 43 | 478 | 3.06e-170 | 485 |
| MS.gene90334.t1 | MTR_7g060840 | 62.470 | 413 | 127 | 9 | 3 | 405 | 43 | 437 | 4.43e-169 | 480 |
| MS.gene90334.t1 | MTR_7g446270 | 62.100 | 438 | 117 | 9 | 4 | 396 | 47 | 480 | 2.06e-162 | 465 |
| MS.gene90334.t1 | MTR_7g060260 | 63.704 | 405 | 129 | 4 | 3 | 405 | 43 | 431 | 8.87e-161 | 459 |
| MS.gene90334.t1 | MTR_7g060020 | 58.273 | 417 | 130 | 8 | 3 | 405 | 46 | 432 | 1.57e-155 | 446 |
| MS.gene90334.t1 | MTR_7g060160 | 54.893 | 419 | 117 | 8 | 3 | 405 | 42 | 404 | 1.20e-139 | 404 |
| MS.gene90334.t1 | MTR_7g060330 | 52.609 | 460 | 147 | 7 | 3 | 405 | 47 | 492 | 1.61e-136 | 400 |
| MS.gene90334.t1 | MTR_7g059510 | 50.000 | 434 | 127 | 12 | 3 | 404 | 46 | 421 | 1.68e-121 | 359 |
| MS.gene90334.t1 | MTR_7g059525 | 54.861 | 288 | 82 | 5 | 158 | 405 | 9 | 288 | 1.68e-92 | 280 |
| MS.gene90334.t1 | MTR_7g060080 | 63.731 | 193 | 68 | 2 | 215 | 405 | 4 | 196 | 5.95e-81 | 247 |
| MS.gene90334.t1 | MTR_8g042170 | 39.101 | 445 | 200 | 15 | 3 | 402 | 41 | 459 | 9.22e-81 | 256 |
| MS.gene90334.t1 | MTR_7g060060 | 62.844 | 218 | 69 | 3 | 10 | 218 | 1 | 215 | 5.11e-80 | 245 |
| MS.gene90334.t1 | MTR_7g059480 | 64.583 | 192 | 66 | 2 | 216 | 405 | 587 | 778 | 2.53e-77 | 254 |
| MS.gene90334.t1 | MTR_7g059480 | 65.072 | 209 | 56 | 3 | 2 | 193 | 41 | 249 | 4.56e-74 | 246 |
| MS.gene90334.t1 | MTR_8g037923 | 35.845 | 438 | 232 | 12 | 3 | 402 | 46 | 472 | 1.42e-76 | 245 |
| MS.gene90334.t1 | MTR_7g059475 | 53.689 | 244 | 88 | 5 | 31 | 249 | 1 | 244 | 5.44e-74 | 231 |
| MS.gene90334.t1 | MTR_7g059515 | 77.241 | 145 | 30 | 1 | 3 | 147 | 42 | 183 | 1.25e-70 | 221 |
| MS.gene90334.t1 | MTR_8g037920 | 34.346 | 428 | 224 | 13 | 9 | 402 | 7 | 411 | 2.87e-68 | 222 |
| MS.gene90334.t1 | MTR_7g059520 | 57.065 | 184 | 69 | 3 | 224 | 405 | 2 | 177 | 1.20e-65 | 207 |
| MS.gene90334.t1 | MTR_8g042400 | 35.967 | 367 | 178 | 9 | 70 | 402 | 3 | 346 | 1.46e-59 | 197 |
| MS.gene90334.t1 | MTR_7g060200 | 56.633 | 196 | 58 | 4 | 31 | 226 | 1 | 169 | 2.70e-48 | 163 |
| MS.gene90334.t1 | MTR_7g060200 | 71.739 | 46 | 11 | 1 | 359 | 404 | 156 | 199 | 2.25e-12 | 66.2 |
| MS.gene90334.t1 | MTR_1g048070 | 52.903 | 155 | 48 | 4 | 3 | 157 | 45 | 174 | 6.64e-38 | 137 |
| MS.gene90334.t1 | MTR_8g042070 | 41.837 | 196 | 97 | 4 | 225 | 403 | 71 | 266 | 2.71e-35 | 131 |
| MS.gene90334.t1 | MTR_8g042080 | 42.754 | 138 | 62 | 4 | 3 | 132 | 41 | 169 | 1.46e-26 | 105 |
| MS.gene90334.t1 | MTR_5g086340 | 26.128 | 421 | 253 | 14 | 3 | 400 | 47 | 432 | 9.35e-26 | 108 |
| MS.gene90334.t1 | MTR_5g016570 | 27.714 | 433 | 256 | 18 | 3 | 401 | 118 | 527 | 1.88e-24 | 105 |
| MS.gene90334.t1 | MTR_8g005235 | 25.248 | 404 | 271 | 11 | 5 | 392 | 44 | 432 | 4.01e-22 | 98.2 |
| MS.gene90334.t1 | MTR_5g086260 | 35.593 | 177 | 107 | 4 | 227 | 400 | 136 | 308 | 6.40e-22 | 95.9 |
| MS.gene90334.t1 | MTR_8g005325 | 27.053 | 414 | 252 | 14 | 5 | 392 | 73 | 462 | 8.36e-21 | 94.4 |
| MS.gene90334.t1 | MTR_8g006525 | 27.649 | 387 | 209 | 13 | 5 | 357 | 45 | 394 | 9.33e-19 | 88.2 |
| MS.gene90334.t1 | MTR_8g005225 | 24.719 | 445 | 256 | 19 | 2 | 402 | 52 | 461 | 3.91e-18 | 86.3 |
| MS.gene90334.t1 | MTR_8g042090 | 59.375 | 64 | 24 | 2 | 278 | 339 | 9 | 72 | 1.06e-16 | 75.9 |
| MS.gene90334.t1 | MTR_6g010810 | 26.392 | 413 | 243 | 16 | 5 | 400 | 39 | 407 | 1.34e-16 | 81.6 |
| MS.gene90334.t1 | MTR_5g087580 | 31.461 | 178 | 114 | 5 | 227 | 401 | 43 | 215 | 6.43e-16 | 76.6 |
| MS.gene90334.t1 | MTR_6g009590 | 25.959 | 339 | 203 | 12 | 5 | 334 | 42 | 341 | 7.67e-16 | 79.3 |
| MS.gene90334.t1 | MTR_2g036320 | 25.059 | 423 | 246 | 19 | 3 | 397 | 82 | 461 | 1.80e-15 | 78.6 |
| MS.gene90334.t1 | MTR_6g010920 | 24.694 | 409 | 247 | 16 | 5 | 397 | 42 | 405 | 7.27e-15 | 76.3 |
| MS.gene90334.t1 | MTR_0024s0080 | 24.927 | 341 | 199 | 11 | 5 | 334 | 42 | 336 | 3.10e-14 | 74.3 |
| MS.gene90334.t1 | MTR_6g009610 | 25.000 | 340 | 205 | 10 | 5 | 334 | 42 | 341 | 3.59e-14 | 73.9 |
| MS.gene90334.t1 | MTR_6g011050 | 25.212 | 353 | 200 | 14 | 5 | 340 | 41 | 346 | 1.17e-13 | 72.4 |
| MS.gene90334.t1 | MTR_6g010890 | 23.284 | 408 | 271 | 12 | 5 | 397 | 42 | 422 | 1.48e-13 | 72.4 |
| MS.gene90334.t1 | MTR_6g009170 | 25.575 | 348 | 220 | 11 | 3 | 344 | 40 | 354 | 1.75e-13 | 72.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90334.t1 | AT3G50120 | 28.345 | 441 | 250 | 21 | 2 | 405 | 117 | 528 | 3.70e-30 | 122 |
| MS.gene90334.t1 | AT3G50140 | 29.291 | 437 | 243 | 19 | 5 | 405 | 130 | 536 | 1.35e-29 | 120 |
| MS.gene90334.t1 | AT3G50150 | 27.816 | 435 | 249 | 17 | 3 | 405 | 105 | 506 | 9.25e-28 | 115 |
| MS.gene90334.t1 | AT3G50170 | 28.372 | 430 | 260 | 17 | 3 | 405 | 128 | 536 | 2.61e-27 | 114 |
| MS.gene90334.t1 | AT3G50180 | 24.766 | 428 | 254 | 15 | 3 | 401 | 193 | 581 | 1.67e-26 | 112 |
| MS.gene90334.t1 | AT3G50130 | 29.070 | 430 | 250 | 19 | 3 | 402 | 154 | 558 | 1.54e-25 | 108 |
| MS.gene90334.t1 | AT3G50160 | 27.400 | 427 | 229 | 17 | 3 | 400 | 119 | 493 | 2.45e-24 | 105 |
| MS.gene90334.t1 | AT3G50190 | 26.950 | 423 | 234 | 16 | 5 | 401 | 113 | 486 | 2.48e-22 | 99.4 |
| MS.gene90334.t1 | AT5G11290 | 25.616 | 406 | 256 | 15 | 5 | 400 | 50 | 419 | 3.69e-20 | 92.0 |
| MS.gene90334.t1 | AT5G22540 | 26.055 | 403 | 227 | 16 | 3 | 376 | 46 | 406 | 2.24e-19 | 90.1 |
| MS.gene90334.t1 | AT2G36430 | 25.459 | 436 | 242 | 16 | 2 | 403 | 59 | 445 | 6.46e-18 | 85.9 |
| MS.gene90334.t1 | AT3G47200 | 25.234 | 428 | 257 | 19 | 3 | 400 | 61 | 455 | 8.60e-18 | 85.5 |
| MS.gene90334.t1 | AT3G47200 | 25.234 | 428 | 257 | 19 | 3 | 400 | 61 | 455 | 8.60e-18 | 85.5 |
| MS.gene90334.t1 | AT3G47250 | 25.000 | 420 | 272 | 18 | 3 | 401 | 76 | 473 | 9.81e-16 | 79.3 |
| MS.gene90334.t1 | AT3G47250 | 25.000 | 420 | 272 | 18 | 3 | 401 | 76 | 473 | 9.81e-16 | 79.3 |
| MS.gene90334.t1 | AT3G47250 | 25.000 | 420 | 272 | 18 | 3 | 401 | 76 | 473 | 9.81e-16 | 79.3 |
| MS.gene90334.t1 | AT4G31980 | 22.983 | 409 | 261 | 14 | 5 | 400 | 310 | 677 | 1.44e-14 | 75.9 |
| MS.gene90334.t1 | AT5G11290 | 24.675 | 385 | 244 | 15 | 26 | 400 | 2 | 350 | 5.41e-13 | 70.1 |
| MS.gene90334.t1 | AT3G47210 | 24.093 | 386 | 181 | 15 | 5 | 343 | 110 | 430 | 3.04e-11 | 65.5 |
Find 74 sgRNAs with CRISPR-Local
Find 97 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGTGTTCTCCTTCTCCTTT+TGG | 0.218497 | 7.4:+43300977 | None:intergenic |
| CTCAATTCCTTCACATCTTC+AGG | 0.253403 | 7.4:+43300247 | None:intergenic |
| AAGTTGTTCACATTGATCTT+AGG | 0.291606 | 7.4:+43300379 | None:intergenic |
| TTGTTCTTATGTATCTTTCA+TGG | 0.304819 | 7.4:-43300287 | MS.gene90334:CDS |
| ATCTTTATGTGTAGTTCCTC+TGG | 0.345713 | 7.4:+43301379 | None:intergenic |
| TCATAGTCGTTCTCAAAATC+TGG | 0.352290 | 7.4:+43300313 | None:intergenic |
| CCCAGGGAAAATGAATATTA+AGG | 0.354844 | 7.4:-43301191 | MS.gene90334:CDS |
| GTGAAGGAATTGAGATCAAA+AGG | 0.357359 | 7.4:-43300238 | MS.gene90334:CDS |
| TCAGATTTGCATTGTTATGA+TGG | 0.360509 | 7.4:+43301464 | None:intergenic |
| GACACATCTCATATGCTATC+AGG | 0.391502 | 7.4:+43300335 | None:intergenic |
| GGAAAGTCTCTTCAAGGCTT+TGG | 0.393152 | 7.4:-43301298 | MS.gene90334:CDS |
| ATGTTCTTTACTACATATCT+TGG | 0.401693 | 7.4:-43301233 | MS.gene90334:CDS |
| AACAATGCAAATCTGAAGTT+AGG | 0.403937 | 7.4:-43301457 | MS.gene90334:CDS |
| TTCACCCGGCTAGCAAATAA+AGG | 0.417622 | 7.4:-43299970 | None:intergenic |
| TTTCCCTGGGTCATCACGCC+TGG | 0.419268 | 7.4:+43301204 | None:intergenic |
| AACTTCCTCATCACTCCAAC+AGG | 0.420645 | 7.4:+43300204 | None:intergenic |
| TCAACTGGAAAGTCTCTTCA+AGG | 0.441120 | 7.4:-43301304 | MS.gene90334:CDS |
| ATCACCTTTATTTGCTAGCC+GGG | 0.470948 | 7.4:+43299966 | None:intergenic |
| ACACCCAAGTTGGTATCAAT+AGG | 0.474116 | 7.4:-43301493 | MS.gene90334:CDS |
| ATTGTATTGTACCTTGGACT+TGG | 0.474271 | 7.4:+43300871 | None:intergenic |
| TCTATGCCCCTGTATGTAAT+CGG | 0.474293 | 7.4:+43300502 | None:intergenic |
| TTTGCATTGTTATGATGGAT+AGG | 0.480031 | 7.4:+43301469 | None:intergenic |
| TCGTGGATGTTGTTCGTGGA+TGG | 0.483877 | 7.4:-43301256 | MS.gene90334:CDS |
| ATGAAGTACTTAAGCTATTG+TGG | 0.484153 | 7.4:-43301111 | MS.gene90334:CDS |
| AGTTCAAGCCGATTACATAC+AGG | 0.485974 | 7.4:-43300510 | MS.gene90334:CDS |
| AATAGCTTAAGTACTTCATA+AGG | 0.489300 | 7.4:+43301115 | None:intergenic |
| TGCAACAAGGAATGCAATAA+CGG | 0.490835 | 7.4:+43300036 | None:intergenic |
| GAGAGAAGTACAAGCTTATG+TGG | 0.495648 | 7.4:-43301435 | MS.gene90334:CDS |
| ACTTTAATAAATACATGACT+TGG | 0.499247 | 7.4:-43300099 | MS.gene90334:CDS |
| TCATGTATTTATTAAAGTAA+TGG | 0.500420 | 7.4:+43300104 | None:intergenic |
| AATCACCTTTATTTGCTAGC+CGG | 0.501703 | 7.4:+43299965 | None:intergenic |
| GTAGGAATAAGACTTAAGTC+AGG | 0.510587 | 7.4:-43300466 | MS.gene90334:CDS |
| ATACATGACTTGGATAGCGC+TGG | 0.511496 | 7.4:-43300089 | MS.gene90334:CDS |
| TGAAGGAATTGAGATCAAAA+GGG | 0.519868 | 7.4:-43300237 | MS.gene90334:CDS |
| TTAAGTCAGGCTTGATACGC+AGG | 0.520908 | 7.4:-43300453 | MS.gene90334:CDS |
| TACATATCTTGGAGAAAGCC+AGG | 0.524353 | 7.4:-43301222 | MS.gene90334:CDS |
| AGTACCTATATTGAAAAGAC+TGG | 0.531460 | 7.4:+43300180 | None:intergenic |
| AGATGAGTCACAGTCAGATA+AGG | 0.536156 | 7.4:-43301014 | MS.gene90334:CDS |
| ATAGAAGATCTAAGAGCAGT+AGG | 0.542239 | 7.4:-43300484 | MS.gene90334:CDS |
| TGATAACATTAATGAACTGA+AGG | 0.542351 | 7.4:-43301353 | MS.gene90334:CDS |
| GAGAAGGTCATGGTTCCAAA+AGG | 0.543574 | 7.4:-43300992 | MS.gene90334:CDS |
| TCAGTGAAATCTATGTCTGT+TGG | 0.544449 | 7.4:+43300430 | None:intergenic |
| AGGACCTATTGATACCAACT+TGG | 0.550349 | 7.4:+43301489 | None:intergenic |
| AATGGTCACGTAATTTCACT+TGG | 0.552535 | 7.4:+43300122 | None:intergenic |
| GACATTGTTTACAGTTCACC+CGG | 0.557924 | 7.4:-43299984 | MS.gene90334:CDS |
| TATCAGGTTGAGTAAAGTAC+AGG | 0.561957 | 7.4:+43300351 | None:intergenic |
| AAAGCCAGGCGTGATGACCC+AGG | 0.566752 | 7.4:-43301208 | MS.gene90334:CDS |
| TACATGACTTGGATAGCGCT+GGG | 0.567810 | 7.4:-43300088 | MS.gene90334:CDS |
| TTATTGGTACACTATGTTCG+AGG | 0.568112 | 7.4:+43301044 | None:intergenic |
| CGACCATCCTGAAGATGTGA+AGG | 0.581217 | 7.4:-43300254 | MS.gene90334:CDS |
| ACATAGATTTCACTGAAGGA+TGG | 0.582435 | 7.4:-43300423 | MS.gene90334:CDS |
| TCTGAATGAAAGTAAGAGCA+AGG | 0.590339 | 7.4:+43300005 | None:intergenic |
| ATTCCTTCACATCTTCAGGA+TGG | 0.602075 | 7.4:+43300251 | None:intergenic |
| AGGAATGCAATAACGGCGCA+AGG | 0.603049 | 7.4:+43300043 | None:intergenic |
| ATGGTCACGTAATTTCACTT+GGG | 0.608057 | 7.4:+43300123 | None:intergenic |
| TTTCAATATAGGTACTGACT+TGG | 0.608589 | 7.4:-43300173 | MS.gene90334:CDS |
| ACAGACATAGATTTCACTGA+AGG | 0.612530 | 7.4:-43300427 | MS.gene90334:CDS |
| GTTCAAGCCGATTACATACA+GGG | 0.613762 | 7.4:-43300509 | MS.gene90334:CDS |
| GGACCTATTGATACCAACTT+GGG | 0.615985 | 7.4:+43301490 | None:intergenic |
| GAAGCACTACACACCCAAGT+TGG | 0.617196 | 7.4:-43301503 | MS.gene90334:CDS |
| GCTGTCGTGGATGTTGTTCG+TGG | 0.618355 | 7.4:-43301260 | MS.gene90334:CDS |
| GCCTTGAAGAAAAGCTGTCG+TGG | 0.621035 | 7.4:-43301273 | MS.gene90334:CDS |
| GTCACAGTCAGATAAGGAGA+AGG | 0.621365 | 7.4:-43301008 | MS.gene90334:CDS |
| GTCATGGTTCCAAAAGGAGA+AGG | 0.623505 | 7.4:-43300986 | MS.gene90334:CDS |
| CATCACTACATCCAAGTCCA+AGG | 0.627091 | 7.4:-43300882 | MS.gene90334:intron |
| GTCAGATAAGGAGAAGGTCA+TGG | 0.628406 | 7.4:-43301002 | MS.gene90334:CDS |
| AGAGAAGTACAAGCTTATGT+GGG | 0.632538 | 7.4:-43301434 | MS.gene90334:CDS |
| CTGAATGAAAGTAAGAGCAA+GGG | 0.633213 | 7.4:+43300006 | None:intergenic |
| GATAACATTAATGAACTGAA+GGG | 0.633288 | 7.4:-43301352 | MS.gene90334:CDS |
| AAGCCAGGCGTGATGACCCA+GGG | 0.636539 | 7.4:-43301207 | MS.gene90334:CDS |
| GTGCACCTGTTGGAGTGATG+AGG | 0.638637 | 7.4:-43300209 | MS.gene90334:CDS |
| CAAGGGCTATAAATGCAACA+AGG | 0.657710 | 7.4:+43300023 | None:intergenic |
| TTCAAGCCGATTACATACAG+GGG | 0.699538 | 7.4:-43300508 | MS.gene90334:CDS |
| AAACATCAAACAGAATCCAG+AGG | 0.706183 | 7.4:-43301395 | MS.gene90334:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCATGTATTTATTAAAGTAA+TGG | + | chr7.4:43301380-43301399 | None:intergenic | 15.0% |
| !!! | ACAAAAGTGATTTTTATTAT+TGG | - | chr7.4:43300906-43300925 | MS.gene90334:CDS | 15.0% |
| !! | ACTTTAATAAATACATGACT+TGG | - | chr7.4:43301382-43301401 | MS.gene90334:CDS | 20.0% |
| !! | TTCTGATGAAAATATAGAAA+CGG | + | chr7.4:43300688-43300707 | None:intergenic | 20.0% |
| !!! | TGATTTTTATTATTGGACAA+AGG | - | chr7.4:43300913-43300932 | MS.gene90334:CDS | 20.0% |
| ! | AATAGCTTAAGTACTTCATA+AGG | + | chr7.4:43300369-43300388 | None:intergenic | 25.0% |
| ! | ATGTTCTTTACTACATATCT+TGG | - | chr7.4:43300248-43300267 | MS.gene90334:CDS | 25.0% |
| ! | GATAACATTAATGAACTGAA+GGG | - | chr7.4:43300129-43300148 | MS.gene90334:CDS | 25.0% |
| ! | TGATAACATTAATGAACTGA+AGG | - | chr7.4:43300128-43300147 | MS.gene90334:CDS | 25.0% |
| ! | TTGTTCTTATGTATCTTTCA+TGG | - | chr7.4:43301194-43301213 | MS.gene90334:CDS | 25.0% |
| AAGTTGTTCACATTGATCTT+AGG | + | chr7.4:43301105-43301124 | None:intergenic | 30.0% | |
| AGTACCTATATTGAAAAGAC+TGG | + | chr7.4:43301304-43301323 | None:intergenic | 30.0% | |
| GTGTTAATTGTATTGTACCT+TGG | + | chr7.4:43300619-43300638 | None:intergenic | 30.0% | |
| TCAGATTTGCATTGTTATGA+TGG | + | chr7.4:43300020-43300039 | None:intergenic | 30.0% | |
| TGAAGGAATTGAGATCAAAA+GGG | - | chr7.4:43301244-43301263 | MS.gene90334:CDS | 30.0% | |
| ! | AACAATGCAAATCTGAAGTT+AGG | - | chr7.4:43300024-43300043 | MS.gene90334:CDS | 30.0% |
| ! | ACCTTAATATTCATTTTCCC+TGG | + | chr7.4:43300294-43300313 | None:intergenic | 30.0% |
| ! | ATGAAGTACTTAAGCTATTG+TGG | - | chr7.4:43300370-43300389 | MS.gene90334:CDS | 30.0% |
| ! | CCTTAATATTCATTTTCCCT+GGG | + | chr7.4:43300293-43300312 | None:intergenic | 30.0% |
| !! | TGATCAACTTTTTCTTGTGA+TGG | - | chr7.4:43300314-43300333 | MS.gene90334:CDS | 30.0% |
| !! | TTTCAATATAGGTACTGACT+TGG | - | chr7.4:43301308-43301327 | MS.gene90334:CDS | 30.0% |
| !! | TTTGCATTGTTATGATGGAT+AGG | + | chr7.4:43300015-43300034 | None:intergenic | 30.0% |
| AAACATCAAACAGAATCCAG+AGG | - | chr7.4:43300086-43300105 | MS.gene90334:CDS | 35.0% | |
| AATGGTCACGTAATTTCACT+TGG | + | chr7.4:43301362-43301381 | None:intergenic | 35.0% | |
| ACAGACATAGATTTCACTGA+AGG | - | chr7.4:43301054-43301073 | MS.gene90334:CDS | 35.0% | |
| ACATAGATTTCACTGAAGGA+TGG | - | chr7.4:43301058-43301077 | MS.gene90334:CDS | 35.0% | |
| ATAGAAGATCTAAGAGCAGT+AGG | - | chr7.4:43300997-43301016 | MS.gene90334:CDS | 35.0% | |
| ATCTTTATGTGTAGTTCCTC+TGG | + | chr7.4:43300105-43300124 | None:intergenic | 35.0% | |
| ATGGTCACGTAATTTCACTT+GGG | + | chr7.4:43301361-43301380 | None:intergenic | 35.0% | |
| ATTGTATTGTACCTTGGACT+TGG | + | chr7.4:43300613-43300632 | None:intergenic | 35.0% | |
| CCCAGGGAAAATGAATATTA+AGG | - | chr7.4:43300290-43300309 | MS.gene90334:CDS | 35.0% | |
| CTGAATGAAAGTAAGAGCAA+GGG | + | chr7.4:43301478-43301497 | None:intergenic | 35.0% | |
| GTAGGAATAAGACTTAAGTC+AGG | - | chr7.4:43301015-43301034 | MS.gene90334:CDS | 35.0% | |
| GTGAAGGAATTGAGATCAAA+AGG | - | chr7.4:43301243-43301262 | MS.gene90334:CDS | 35.0% | |
| TCAGTGAAATCTATGTCTGT+TGG | + | chr7.4:43301054-43301073 | None:intergenic | 35.0% | |
| TCATAGTCGTTCTCAAAATC+TGG | + | chr7.4:43301171-43301190 | None:intergenic | 35.0% | |
| TCTGAATGAAAGTAAGAGCA+AGG | + | chr7.4:43301479-43301498 | None:intergenic | 35.0% | |
| TGCAACAAGGAATGCAATAA+CGG | + | chr7.4:43301448-43301467 | None:intergenic | 35.0% | |
| TGTTTCAAATTCTGCCCTTT+GGG | + | chr7.4:43300651-43300670 | None:intergenic | 35.0% | |
| TTATTGGTACACTATGTTCG+AGG | + | chr7.4:43300440-43300459 | None:intergenic | 35.0% | |
| ! | AGAGAAGTACAAGCTTATGT+GGG | - | chr7.4:43300047-43300066 | MS.gene90334:CDS | 35.0% |
| ! | GTTGCCAGTCTTTTCAATAT+AGG | - | chr7.4:43301297-43301316 | MS.gene90334:CDS | 35.0% |
| !! | AACACTAAAAAGCACCCAAA+GGG | - | chr7.4:43300634-43300653 | MS.gene90334:intron | 35.0% |
| !! | GACTCATCTGCTTTTGTTAT+TGG | + | chr7.4:43300456-43300475 | None:intergenic | 35.0% |
| !! | TAACACTAAAAAGCACCCAA+AGG | - | chr7.4:43300633-43300652 | MS.gene90334:intron | 35.0% |
| !! | TATCAGGTTGAGTAAAGTAC+AGG | + | chr7.4:43301133-43301152 | None:intergenic | 35.0% |
| AGATGAGTCACAGTCAGATA+AGG | - | chr7.4:43300467-43300486 | MS.gene90334:CDS | 40.0% | |
| AGGACCTATTGATACCAACT+TGG | + | chr7.4:43299995-43300014 | None:intergenic | 40.0% | |
| AGTTCAAGCCGATTACATAC+AGG | - | chr7.4:43300971-43300990 | MS.gene90334:CDS | 40.0% | |
| ATTCCTTCACATCTTCAGGA+TGG | + | chr7.4:43301233-43301252 | None:intergenic | 40.0% | |
| CAAGGGCTATAAATGCAACA+AGG | + | chr7.4:43301461-43301480 | None:intergenic | 40.0% | |
| CTCAATTCCTTCACATCTTC+AGG | + | chr7.4:43301237-43301256 | None:intergenic | 40.0% | |
| CTGTTTCAAATTCTGCCCTT+TGG | + | chr7.4:43300652-43300671 | None:intergenic | 40.0% | |
| GACACATCTCATATGCTATC+AGG | + | chr7.4:43301149-43301168 | None:intergenic | 40.0% | |
| GACATTGTTTACAGTTCACC+CGG | - | chr7.4:43301497-43301516 | MS.gene90334:CDS | 40.0% | |
| GGACCTATTGATACCAACTT+GGG | + | chr7.4:43299994-43300013 | None:intergenic | 40.0% | |
| GTTCAAGCCGATTACATACA+GGG | - | chr7.4:43300972-43300991 | MS.gene90334:CDS | 40.0% | |
| TACATATCTTGGAGAAAGCC+AGG | - | chr7.4:43300259-43300278 | MS.gene90334:CDS | 40.0% | |
| TCAACTGGAAAGTCTCTTCA+AGG | - | chr7.4:43300177-43300196 | MS.gene90334:CDS | 40.0% | |
| TCTATGCCCCTGTATGTAAT+CGG | + | chr7.4:43300982-43301001 | None:intergenic | 40.0% | |
| TTCAAGCCGATTACATACAG+GGG | - | chr7.4:43300973-43300992 | MS.gene90334:CDS | 40.0% | |
| ! | GAGAGAAGTACAAGCTTATG+TGG | - | chr7.4:43300046-43300065 | MS.gene90334:CDS | 40.0% |
| ! | GATGGTAGATGTGCTTTTGT+TGG | - | chr7.4:43300332-43300351 | MS.gene90334:CDS | 40.0% |
| !! | ACACCCAAGTTGGTATCAAT+AGG | - | chr7.4:43299988-43300007 | MS.gene90334:CDS | 40.0% |
| !!! | GACGAGGTTTTAACTTCAAC+TGG | - | chr7.4:43300162-43300181 | MS.gene90334:CDS | 40.0% |
| AACTTCCTCATCACTCCAAC+AGG | + | chr7.4:43301280-43301299 | None:intergenic | 45.0% | |
| ATGTTATCTCCGAGTGACAC+CGG | + | chr7.4:43300861-43300880 | None:intergenic | 45.0% | |
| CATCACTACATCCAAGTCCA+AGG | - | chr7.4:43300599-43300618 | MS.gene90334:intron | 45.0% | |
| GAGAAGGTCATGGTTCCAAA+AGG | - | chr7.4:43300489-43300508 | MS.gene90334:CDS | 45.0% | |
| GGAAAGTCTCTTCAAGGCTT+TGG | - | chr7.4:43300183-43300202 | MS.gene90334:CDS | 45.0% | |
| GTCACAGTCAGATAAGGAGA+AGG | - | chr7.4:43300473-43300492 | MS.gene90334:CDS | 45.0% | |
| GTCAGATAAGGAGAAGGTCA+TGG | - | chr7.4:43300479-43300498 | MS.gene90334:CDS | 45.0% | |
| GTCATGGTTCCAAAAGGAGA+AGG | - | chr7.4:43300495-43300514 | MS.gene90334:CDS | 45.0% | |
| TTAAGTCAGGCTTGATACGC+AGG | - | chr7.4:43301028-43301047 | MS.gene90334:CDS | 45.0% | |
| ! | AAGGGATTTTGTGCACCTGT+TGG | - | chr7.4:43301262-43301281 | MS.gene90334:CDS | 45.0% |
| ! | AATTTTGGATCCTAAGCCCC+CGG | - | chr7.4:43300839-43300858 | MS.gene90334:intron | 45.0% |
| ! | ATACATGACTTGGATAGCGC+TGG | - | chr7.4:43301392-43301411 | MS.gene90334:CDS | 45.0% |
| ! | CTGTGTTCTCCTTCTCCTTT+TGG | + | chr7.4:43300507-43300526 | None:intergenic | 45.0% |
| ! | TACATGACTTGGATAGCGCT+GGG | - | chr7.4:43301393-43301412 | MS.gene90334:CDS | 45.0% |
| ! | TCCACGACAGCTTTTCTTCA+AGG | + | chr7.4:43300212-43300231 | None:intergenic | 45.0% |
| !!! | ATAAAATAATTTCTTAATTT+TGG | - | chr7.4:43300824-43300843 | MS.gene90334:intron | 5.0% |
| AGGAATGCAATAACGGCGCA+AGG | + | chr7.4:43301441-43301460 | None:intergenic | 50.0% | |
| CGACCATCCTGAAGATGTGA+AGG | - | chr7.4:43301227-43301246 | MS.gene90334:CDS | 50.0% | |
| GCCTTGAAGAAAAGCTGTCG+TGG | - | chr7.4:43300208-43300227 | MS.gene90334:CDS | 50.0% | |
| TCGTGGATGTTGTTCGTGGA+TGG | - | chr7.4:43300225-43300244 | MS.gene90334:CDS | 50.0% | |
| TGTTATCTCCGAGTGACACC+GGG | + | chr7.4:43300860-43300879 | None:intergenic | 50.0% | |
| ! | GAAGCACTACACACCCAAGT+TGG | - | chr7.4:43299978-43299997 | MS.gene90334:CDS | 50.0% |
| GCTGTCGTGGATGTTGTTCG+TGG | - | chr7.4:43300221-43300240 | MS.gene90334:CDS | 55.0% | |
| GTGCACCTGTTGGAGTGATG+AGG | - | chr7.4:43301272-43301291 | MS.gene90334:CDS | 55.0% | |
| GTTATCTCCGAGTGACACCG+GGG | + | chr7.4:43300859-43300878 | None:intergenic | 55.0% | |
| TTATCTCCGAGTGACACCGG+GGG | + | chr7.4:43300858-43300877 | None:intergenic | 55.0% | |
| !! | GAAGGGTCGTTTTAGCGACG+AGG | - | chr7.4:43300146-43300165 | MS.gene90334:CDS | 55.0% |
| AAAGCCAGGCGTGATGACCC+AGG | - | chr7.4:43300273-43300292 | MS.gene90334:CDS | 60.0% | |
| AAGCCAGGCGTGATGACCCA+GGG | - | chr7.4:43300274-43300293 | MS.gene90334:CDS | 60.0% | |
| TTTCCCTGGGTCATCACGCC+TGG | + | chr7.4:43300280-43300299 | None:intergenic | 60.0% | |
| !! | CCTAAGCCCCCGGTGTCACT+CGG | - | chr7.4:43300849-43300868 | MS.gene90334:intron | 65.0% |
| CCGAGTGACACCGGGGGCTT+AGG | + | chr7.4:43300852-43300871 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 43299973 | 43301530 | 43299973 | ID=MS.gene90334 |
| chr7.4 | mRNA | 43299973 | 43301530 | 43299973 | ID=MS.gene90334.t1;Parent=MS.gene90334 |
| chr7.4 | exon | 43300883 | 43301530 | 43300883 | ID=MS.gene90334.t1.exon1;Parent=MS.gene90334.t1 |
| chr7.4 | CDS | 43300883 | 43301530 | 43300883 | ID=cds.MS.gene90334.t1;Parent=MS.gene90334.t1 |
| chr7.4 | exon | 43299973 | 43300542 | 43299973 | ID=MS.gene90334.t1.exon2;Parent=MS.gene90334.t1 |
| chr7.4 | CDS | 43299973 | 43300542 | 43299973 | ID=cds.MS.gene90334.t1;Parent=MS.gene90334.t1 |
| Gene Sequence |
| Protein sequence |
