AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68821

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68821.t1 MTR_2g450310 83.451 284 47 0 1 284 1 284 5.60e-176 495
MS.gene68821.t1 MTR_2g450510 79.225 284 59 0 1 284 1 284 9.10e-168 475
MS.gene68821.t1 MTR_1088s0010 95.238 147 7 0 1 147 1 147 1.56e-103 298
MS.gene68821.t1 MTR_2g020630 38.328 287 171 4 1 284 10 293 3.41e-69 223
MS.gene68821.t1 MTR_4g124800 41.418 268 153 4 21 284 29 296 3.51e-64 210
MS.gene68821.t1 MTR_2g043850 38.148 270 161 4 4 270 13 279 3.09e-61 202
MS.gene68821.t1 MTR_2g044140 37.778 270 162 4 4 270 13 279 9.17e-61 201
MS.gene68821.t1 MTR_1372s0010 92.405 79 6 0 147 225 1 79 8.32e-49 157
MS.gene68821.t1 MTR_1308s0010 91.379 58 5 0 227 284 1 58 2.46e-32 117
MS.gene68821.t1 MTR_7g107710 27.985 268 153 11 2 265 3 234 8.99e-18 82.8
MS.gene68821.t1 MTR_8g037880 27.381 252 126 9 5 232 4 222 8.56e-17 80.1
MS.gene68821.t1 MTR_8g037910 26.190 252 128 9 5 232 4 221 2.62e-16 77.4
MS.gene68821.t1 MTR_8g037910 26.190 252 128 9 5 232 4 221 3.04e-16 77.8
MS.gene68821.t1 MTR_8g037910 25.397 252 130 8 5 232 4 221 8.32e-16 77.4
MS.gene68821.t1 MTR_1g069275 27.778 234 132 10 7 239 27 224 1.80e-15 76.3
MS.gene68821.t1 MTR_4g051642 26.866 268 157 11 1 265 3 234 1.21e-13 70.9
MS.gene68821.t1 MTR_1g046230 27.046 281 160 15 6 281 25 265 2.11e-13 70.1
MS.gene68821.t1 MTR_3g437580 26.587 252 136 8 3 229 11 238 5.70e-13 68.6
MS.gene68821.t1 MTR_3g437580 26.587 252 136 8 3 229 11 238 6.98e-13 68.6
MS.gene68821.t1 MTR_3g109520 25.105 239 135 9 6 239 40 239 3.96e-12 66.2
MS.gene68821.t1 MTR_1g008380 24.576 236 137 11 7 239 44 241 1.09e-11 65.1
MS.gene68821.t1 MTR_3g088925 24.815 270 160 10 1 265 3 234 6.70e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68821.t1 AT1G19250 37.895 285 169 5 4 284 12 292 1.29e-64 211
MS.gene68821.t1 AT5G45180 39.526 253 141 7 4 251 12 257 6.34e-59 194
MS.gene68821.t1 AT1G62560 25.197 254 131 10 3 229 11 232 1.56e-14 73.6
MS.gene68821.t1 AT1G62560 25.197 254 131 10 3 229 11 232 2.04e-14 73.2
MS.gene68821.t1 AT1G62620 25.000 252 135 9 4 229 12 235 3.18e-14 72.4
MS.gene68821.t1 AT1G62620 25.000 252 135 9 4 229 12 235 5.32e-14 72.0
MS.gene68821.t1 AT1G63370 25.000 252 135 7 4 229 12 235 6.83e-14 71.2
MS.gene68821.t1 AT1G63370 25.000 252 135 7 4 229 12 235 1.18e-13 71.2
MS.gene68821.t1 AT4G28720 27.386 241 129 12 6 239 26 227 4.94e-13 69.3
MS.gene68821.t1 AT1G65860 22.327 318 173 11 3 279 11 295 7.91e-13 68.6
MS.gene68821.t1 AT4G32540 25.896 251 134 12 7 251 23 227 2.95e-12 66.6
MS.gene68821.t1 AT4G32540 25.896 251 134 12 7 251 23 227 3.11e-12 66.6
MS.gene68821.t1 AT1G48910 27.197 239 135 9 1 237 1 202 3.82e-12 66.2
MS.gene68821.t1 AT1G12200 25.197 254 135 7 3 229 12 237 4.46e-12 66.2
MS.gene68821.t1 AT1G12200 25.197 254 135 7 3 229 12 237 5.62e-12 66.2
MS.gene68821.t1 AT1G21430 27.706 231 128 8 1 226 1 197 3.93e-11 63.5
MS.gene68821.t1 AT4G13260 24.096 249 145 12 7 251 30 238 8.62e-11 62.0

Find 57 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTTACCTTTAAAGATTTC+TGG 0.118162 2.2:-33086366 None:intergenic
TGCAAATATGCTCTACAAAT+TGG 0.214406 2.2:+33085277 MS.gene68821:CDS
GGAAGTCTTGTTTGTTAGTT+TGG 0.241397 2.2:-33085375 None:intergenic
TGGAAGTCCATTTGGTTCTA+AGG 0.280378 2.2:+33085594 MS.gene68821:CDS
AGGTAAAGTTATGCATTCTT+TGG 0.285374 2.2:+33086380 MS.gene68821:CDS
AAATGAAGTCATGGGAATTA+TGG 0.290096 2.2:+33085563 MS.gene68821:CDS
GAAACCAGTGTGTAGTTCTT+TGG 0.303585 2.2:-33086661 None:intergenic
ACGATGGCATTGGTGGAATA+TGG 0.325013 2.2:+33085326 MS.gene68821:CDS
TCTTGCACTTGTTGATTACT+TGG 0.340440 2.2:-33085442 None:intergenic
AGCTGCAGTCTTGTTGTCTA+AGG 0.341399 2.2:-33086412 None:intergenic
GACATTGCGGGCATTAATCT+TGG 0.343163 2.2:+33086695 MS.gene68821:CDS
CTTTACCTTTAAAGATTTCT+GGG 0.345088 2.2:-33086365 None:intergenic
AAAGGCCCAGAAATCTTTAA+AGG 0.357841 2.2:+33086360 MS.gene68821:CDS
CCCATGGAAAATCTGTAAAC+TGG 0.363372 2.2:-33085396 None:intergenic
TCATTGATATTGATTATGTT+GGG 0.373374 2.2:+33085527 MS.gene68821:CDS
TTGGAATTGAATCTTATGTA+TGG 0.382205 2.2:-33085502 None:intergenic
TTGTTTGTTAGTTTGGAGTT+TGG 0.394924 2.2:-33085368 None:intergenic
GTCATTGATATTGATTATGT+TGG 0.404807 2.2:+33085526 MS.gene68821:CDS
CTTTGCATTGGAAAGTATAG+TGG 0.412036 2.2:+33086306 MS.gene68821:CDS
AATTTGTAGAGCATATTTGC+AGG 0.413362 2.2:-33085275 None:intergenic
ATGCCCGCAATGTCAAAATC+TGG 0.420705 2.2:-33086686 None:intergenic
CATTTGGTTCTAAGGGAACT+TGG 0.423541 2.2:+33085602 MS.gene68821:CDS
AGAGTTACAATAGTAGGCTC+AGG 0.434337 2.2:+33086453 MS.gene68821:CDS
ATAATCAATATCAATGACTT+TGG 0.438062 2.2:-33085521 None:intergenic
TGGGAATTATGGAGTGGTAA+TGG 0.443970 2.2:+33085574 MS.gene68821:CDS
AAGTTTGTCATTCTTTGCAT+TGG 0.451719 2.2:+33086294 MS.gene68821:CDS
AACAAGAGAGTTACAATAGT+AGG 0.452094 2.2:+33086447 MS.gene68821:CDS
AGTGGTAATGGAAGTCCATT+TGG 0.462695 2.2:+33085586 MS.gene68821:CDS
GGAAGTCCATTTGGTTCTAA+GGG 0.467978 2.2:+33085595 MS.gene68821:CDS
TCGTCAGATTCGTAGACAAT+TGG 0.475201 2.2:-33085307 None:intergenic
GGCCACTTTACTTTCACCAT+TGG 0.477134 2.2:+33086790 MS.gene68821:CDS
GAATCTTATGTATGGAATGA+TGG 0.480663 2.2:-33085494 None:intergenic
AATGTTGCAATTGTTGGGGC+AGG 0.496768 2.2:+33085235 MS.gene68821:CDS
GAATCTGACGATGGCATTGG+TGG 0.504538 2.2:+33085319 MS.gene68821:CDS
CTCATGAAGAAATGAAGTCA+TGG 0.511087 2.2:+33085554 MS.gene68821:CDS
GAAATTCTTAGTATCTTCTA+CGG 0.519004 2.2:-33085632 None:intergenic
AAGTGGCCACAAGGCTAAGT+AGG 0.524274 2.2:-33086775 None:intergenic
CTAAGTAGGAATGGTTCTCC+AGG 0.528761 2.2:-33086761 None:intergenic
CAAGTTCCCTTAGAACCAAA+TGG 0.541301 2.2:-33085601 None:intergenic
ATTGTTGGGGCAGGAATAAG+TGG 0.555531 2.2:+33085244 MS.gene68821:CDS
CATTGATATTGATTATGTTG+GGG 0.562755 2.2:+33085528 MS.gene68821:CDS
TCATGAAGAAATGAAGTCAT+GGG 0.573176 2.2:+33085555 MS.gene68821:CDS
GCTGAATGTGCAAATGCAAA+TGG 0.573735 2.2:+33086498 MS.gene68821:CDS
TACCAATGGTGAAAGTAAAG+TGG 0.578127 2.2:-33086792 None:intergenic
GCCACAAGGCTAAGTAGGAA+TGG 0.579646 2.2:-33086770 None:intergenic
TACGAATCTGACGATGGCAT+TGG 0.589087 2.2:+33085316 MS.gene68821:CDS
GTACTAAAAGCTCCGCAAAA+CGG 0.593919 2.2:-33086733 None:intergenic
GAGTTACAATAGTAGGCTCA+GGG 0.602052 2.2:+33086454 MS.gene68821:CDS
ACCATTCCTACTTAGCCTTG+TGG 0.606013 2.2:+33086769 MS.gene68821:CDS
GTGAAAGTAAAGTGGCCACA+AGG 0.620197 2.2:-33086784 None:intergenic
TTTGTAAATTCAGGTTCACA+AGG 0.623646 2.2:+33086269 MS.gene68821:intron
GTTCTTTGGATAATTGTGCA+AGG 0.631927 2.2:-33086647 None:intergenic
AAGTCATGGGAATTATGGAG+TGG 0.662418 2.2:+33085568 MS.gene68821:CDS
TTCATATTTACATACCTCGG+TGG 0.678741 2.2:-33085656 None:intergenic
ATTGTCTACGAATCTGACGA+TGG 0.682809 2.2:+33085310 MS.gene68821:CDS
TTATCCAAAGAACTACACAC+TGG 0.687255 2.2:+33086657 MS.gene68821:CDS
TACTAAGAATTTCACCACCG+AGG 0.721507 2.2:+33085642 MS.gene68821:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAAATATTTAATTAGCTTA+AGG + chr2.2:33086185-33086204 MS.gene68821:intron 10.0%
!!! TTCCTTTTTTTTTTTTTTAT+GGG - chr2.2:33086219-33086238 None:intergenic 10.0%
!!! TTTAAGACAAATTTTATAAT+GGG + chr2.2:33085865-33085884 MS.gene68821:intron 10.0%
!!! TTTCCTTTTTTTTTTTTTTA+TGG - chr2.2:33086220-33086239 None:intergenic 10.0%
!!! TTTTTTTATTCAAAATTTGA+AGG + chr2.2:33086015-33086034 MS.gene68821:intron 10.0%
!! TTCCCATAAAAAAAAAAAAA+AGG + chr2.2:33086214-33086233 MS.gene68821:intron 15.0%
!!! GTTTAAGACAAATTTTATAA+TGG + chr2.2:33085864-33085883 MS.gene68821:intron 15.0%
!!! TTCATATTTTTTTCTTGTTT+GGG + chr2.2:33085725-33085744 MS.gene68821:intron 15.0%
!!! TTTCATATTTTTTTCTTGTT+TGG + chr2.2:33085724-33085743 MS.gene68821:intron 15.0%
!! AAAAAAATGTTGCAATTGTT+GGG + chr2.2:33085230-33085249 MS.gene68821:CDS 20.0%
!! ATAATCAATATCAATGACTT+TGG - chr2.2:33085524-33085543 None:intergenic 20.0%
!! GTTTGAATATTTGTAAATTC+AGG + chr2.2:33086260-33086279 MS.gene68821:intron 20.0%
!!! CTTCTTTTTCTTTTTCATTT+TGG + chr2.2:33085697-33085716 MS.gene68821:intron 20.0%
!!! TCATTGATATTGATTATGTT+GGG + chr2.2:33085527-33085546 MS.gene68821:CDS 20.0%
! AAAAAATGTTGCAATTGTTG+GGG + chr2.2:33085231-33085250 MS.gene68821:CDS 25.0%
! AAGAAAAAATCAAATTGCAG+TGG + chr2.2:33085794-33085813 MS.gene68821:intron 25.0%
! ACTACACAAATAAACATGAA+AGG - chr2.2:33086094-33086113 None:intergenic 25.0%
! ATGTTTATTTGTGTAGTTGT+TGG + chr2.2:33086097-33086116 MS.gene68821:intron 25.0%
! ATTTATTCACGTGTATAGTA+TGG - chr2.2:33086146-33086165 None:intergenic 25.0%
! CAATTCATATTTACATACCT+CGG - chr2.2:33085662-33085681 None:intergenic 25.0%
! CTACACAAATAAACATGAAA+GGG - chr2.2:33086093-33086112 None:intergenic 25.0%
! GAAAAAAATGTTGCAATTGT+TGG + chr2.2:33085229-33085248 MS.gene68821:CDS 25.0%
! GAAATTCTTAGTATCTTCTA+CGG - chr2.2:33085635-33085654 None:intergenic 25.0%
! TTGGAATTGAATCTTATGTA+TGG - chr2.2:33085505-33085524 None:intergenic 25.0%
!! AATCAATTTTTCACCTCTAT+AGG + chr2.2:33086614-33086633 MS.gene68821:intron 25.0%
!! ACTTTACCTTTAAAGATTTC+TGG - chr2.2:33086369-33086388 None:intergenic 25.0%
!! CATTGATATTGATTATGTTG+GGG + chr2.2:33085528-33085547 MS.gene68821:CDS 25.0%
!! CTTTACCTTTAAAGATTTCT+GGG - chr2.2:33086368-33086387 None:intergenic 25.0%
!! GTCATTGATATTGATTATGT+TGG + chr2.2:33085526-33085545 MS.gene68821:CDS 25.0%
AAAAAATCAAATTGCAGTGG+TGG + chr2.2:33085797-33085816 MS.gene68821:intron 30.0%
AAATGAAGTCATGGGAATTA+TGG + chr2.2:33085563-33085582 MS.gene68821:CDS 30.0%
AACAAGAGAGTTACAATAGT+AGG + chr2.2:33086447-33086466 MS.gene68821:CDS 30.0%
AAGTTTGTCATTCTTTGCAT+TGG + chr2.2:33086294-33086313 MS.gene68821:CDS 30.0%
AATTTGTAGAGCATATTTGC+AGG - chr2.2:33085278-33085297 None:intergenic 30.0%
AGGTAAAGTTATGCATTCTT+TGG + chr2.2:33086380-33086399 MS.gene68821:CDS 30.0%
CTCTATACACTGTACAATTA+TGG - chr2.2:33086539-33086558 None:intergenic 30.0%
GAATCTTATGTATGGAATGA+TGG - chr2.2:33085497-33085516 None:intergenic 30.0%
TCATGAAGAAATGAAGTCAT+GGG + chr2.2:33085555-33085574 MS.gene68821:CDS 30.0%
TGCAAATATGCTCTACAAAT+TGG + chr2.2:33085277-33085296 MS.gene68821:CDS 30.0%
! CTTATATTTCAACCGTTTTG+CGG + chr2.2:33086721-33086740 MS.gene68821:CDS 30.0%
! GAAGGTTTAAAACCCTAATT+TGG + chr2.2:33086033-33086052 MS.gene68821:intron 30.0%
! TCTTTTATAGAAGAATCCCA+TGG - chr2.2:33085415-33085434 None:intergenic 30.0%
! TTGTTTGTTAGTTTGGAGTT+TGG - chr2.2:33085371-33085390 None:intergenic 30.0%
! TTTGTAAATTCAGGTTCACA+AGG + chr2.2:33086269-33086288 MS.gene68821:intron 30.0%
!! AAATGTTTCTCTAGCTTTGT+TGG - chr2.2:33085942-33085961 None:intergenic 30.0%
AAACCCTAATTTGGATTGCT+TGG + chr2.2:33086042-33086061 MS.gene68821:intron 35.0%
AAAGGCCCAGAAATCTTTAA+AGG + chr2.2:33086360-33086379 MS.gene68821:CDS 35.0%
CACAAATAAACATGAAAGGG+AGG - chr2.2:33086090-33086109 None:intergenic 35.0%
CTCATGAAGAAATGAAGTCA+TGG + chr2.2:33085554-33085573 MS.gene68821:CDS 35.0%
GATGGAAAAATGTTGAGCAT+AGG - chr2.2:33085479-33085498 None:intergenic 35.0%
GCTAGAGAAACATTTGTAGT+TGG + chr2.2:33085947-33085966 MS.gene68821:intron 35.0%
GTATAGTATGGTGTTCGATA+AGG - chr2.2:33086134-33086153 None:intergenic 35.0%
GTTCTTTGGATAATTGTGCA+AGG - chr2.2:33086650-33086669 None:intergenic 35.0%
TCTTAAACCTCCAAACATGT+TGG - chr2.2:33085852-33085871 None:intergenic 35.0%
TGGTGTTCGATAAGGTAAAA+TGG - chr2.2:33086126-33086145 None:intergenic 35.0%
TTATCCAAAGAACTACACAC+TGG + chr2.2:33086657-33086676 MS.gene68821:CDS 35.0%
TTCATATTTACATACCTCGG+TGG - chr2.2:33085659-33085678 None:intergenic 35.0%
! CCAGTTTACAGATTTTCCAT+GGG + chr2.2:33085396-33085415 MS.gene68821:CDS 35.0%
! CTTTGCATTGGAAAGTATAG+TGG + chr2.2:33086306-33086325 MS.gene68821:CDS 35.0%
! TCCAGTTTACAGATTTTCCA+TGG + chr2.2:33085395-33085414 MS.gene68821:CDS 35.0%
! TCTTGCACTTGTTGATTACT+TGG - chr2.2:33085445-33085464 None:intergenic 35.0%
!! AGCTTTGTTGGTTAGAAAGA+AGG - chr2.2:33085930-33085949 None:intergenic 35.0%
!! GGAAGTCTTGTTTGTTAGTT+TGG - chr2.2:33085378-33085397 None:intergenic 35.0%
!! TCTTCCAGATTTTGACATTG+CGG + chr2.2:33086682-33086701 MS.gene68821:CDS 35.0%
AAGTCATGGGAATTATGGAG+TGG + chr2.2:33085568-33085587 MS.gene68821:CDS 40.0%
AATATTCCTAAGTTCCCCCT+TGG + chr2.2:33086336-33086355 MS.gene68821:CDS 40.0%
AGAGTTACAATAGTAGGCTC+AGG + chr2.2:33086453-33086472 MS.gene68821:CDS 40.0%
AGGGGGAACTTAGGAATATT+TGG - chr2.2:33086336-33086355 None:intergenic 40.0%
ATTGTCTACGAATCTGACGA+TGG + chr2.2:33085310-33085329 MS.gene68821:CDS 40.0%
CAAGTTCCCTTAGAACCAAA+TGG - chr2.2:33085604-33085623 None:intergenic 40.0%
CAGTGGTGGTTAGAACTATT+CGG + chr2.2:33085811-33085830 MS.gene68821:intron 40.0%
CCCATGGAAAATCTGTAAAC+TGG - chr2.2:33085399-33085418 None:intergenic 40.0%
GAAACCAGTGTGTAGTTCTT+TGG - chr2.2:33086664-33086683 None:intergenic 40.0%
GAGTTACAATAGTAGGCTCA+GGG + chr2.2:33086454-33086473 MS.gene68821:CDS 40.0%
GCTGAATGTGCAAATGCAAA+TGG + chr2.2:33086498-33086517 MS.gene68821:CDS 40.0%
GTACTAAAAGCTCCGCAAAA+CGG - chr2.2:33086736-33086755 None:intergenic 40.0%
TACTAAGAATTTCACCACCG+AGG + chr2.2:33085642-33085661 MS.gene68821:CDS 40.0%
TAGAACTATTCGGACTCACA+TGG + chr2.2:33085821-33085840 MS.gene68821:intron 40.0%
TCGTCAGATTCGTAGACAAT+TGG - chr2.2:33085310-33085329 None:intergenic 40.0%
TGGGAATTATGGAGTGGTAA+TGG + chr2.2:33085574-33085593 MS.gene68821:CDS 40.0%
!! AGTGGTAATGGAAGTCCATT+TGG + chr2.2:33085586-33085605 MS.gene68821:CDS 40.0%
!! CATTTGGTTCTAAGGGAACT+TGG + chr2.2:33085602-33085621 MS.gene68821:CDS 40.0%
!! CTTCCAGATTTTGACATTGC+GGG + chr2.2:33086683-33086702 MS.gene68821:CDS 40.0%
!! GGAAGTCCATTTGGTTCTAA+GGG + chr2.2:33085595-33085614 MS.gene68821:CDS 40.0%
!! TGGAAGTCCATTTGGTTCTA+AGG + chr2.2:33085594-33085613 MS.gene68821:CDS 40.0%
AACCTCCAAACATGTTGGAC+CGG - chr2.2:33085847-33085866 None:intergenic 45.0%
AATGTTGCAATTGTTGGGGC+AGG + chr2.2:33085235-33085254 MS.gene68821:CDS 45.0%
AGGATGAGTGACTCCTATAG+AGG - chr2.2:33086630-33086649 None:intergenic 45.0%
ATGAAAGGGAGGTCGTGTTA+TGG - chr2.2:33086079-33086098 None:intergenic 45.0%
ATGCCCGCAATGTCAAAATC+TGG - chr2.2:33086689-33086708 None:intergenic 45.0%
ATTGTTGGGGCAGGAATAAG+TGG + chr2.2:33085244-33085263 MS.gene68821:CDS 45.0%
CTAAGTAGGAATGGTTCTCC+AGG - chr2.2:33086764-33086783 None:intergenic 45.0%
GACATTGCGGGCATTAATCT+TGG + chr2.2:33086695-33086714 MS.gene68821:CDS 45.0%
GGGCCAAGCAATCCAAATTA+GGG - chr2.2:33086048-33086067 None:intergenic 45.0%
GTGAAAGTAAAGTGGCCACA+AGG - chr2.2:33086787-33086806 None:intergenic 45.0%
TACGAATCTGACGATGGCAT+TGG + chr2.2:33085316-33085335 MS.gene68821:CDS 45.0%
TGAAAGGGAGGTCGTGTTAT+GGG - chr2.2:33086078-33086097 None:intergenic 45.0%
! GAGCTTTTAGTACACAAGCC+TGG + chr2.2:33086743-33086762 MS.gene68821:CDS 45.0%
!! ACCATTCCTACTTAGCCTTG+TGG + chr2.2:33086769-33086788 MS.gene68821:CDS 45.0%
!! ACGATGGCATTGGTGGAATA+TGG + chr2.2:33085326-33085345 MS.gene68821:CDS 45.0%
!! AGATTTCTGGGCCTTTTCCA+AGG - chr2.2:33086356-33086375 None:intergenic 45.0%
!! AGCTGCAGTCTTGTTGTCTA+AGG - chr2.2:33086415-33086434 None:intergenic 45.0%
!! ATTTCTGGGCCTTTTCCAAG+GGG - chr2.2:33086354-33086373 None:intergenic 45.0%
!! GATTTCTGGGCCTTTTCCAA+GGG - chr2.2:33086355-33086374 None:intergenic 45.0%
!!! TTTTTTTCATTTATTTTATA+AGG - chr2.2:33085892-33085911 None:intergenic 5.0%
AAGTGGCCACAAGGCTAAGT+AGG - chr2.2:33086778-33086797 None:intergenic 50.0%
ACTATTCGGACTCACATGGC+CGG + chr2.2:33085825-33085844 MS.gene68821:intron 50.0%
AGGTCGTGTTATGGGTTCGT+TGG - chr2.2:33086070-33086089 None:intergenic 50.0%
CCTAAGTTCCCCCTTGGAAA+AGG + chr2.2:33086342-33086361 MS.gene68821:CDS 50.0%
GCCACAAGGCTAAGTAGGAA+TGG - chr2.2:33086773-33086792 None:intergenic 50.0%
GGGGCCAAGCAATCCAAATT+AGG - chr2.2:33086049-33086068 None:intergenic 50.0%
GGTCGTGTTATGGGTTCGTT+GGG - chr2.2:33086069-33086088 None:intergenic 50.0%
GTCGTGTTATGGGTTCGTTG+GGG - chr2.2:33086068-33086087 None:intergenic 50.0%
! CCTTTTCCAAGGGGGAACTT+AGG - chr2.2:33086345-33086364 None:intergenic 50.0%
! GAATCTGACGATGGCATTGG+TGG + chr2.2:33085319-33085338 MS.gene68821:CDS 50.0%
! TTTCTGGGCCTTTTCCAAGG+GGG - chr2.2:33086353-33086372 None:intergenic 50.0%
CATGGCCGGTCCAACATGTT+TGG + chr2.2:33085839-33085858 MS.gene68821:intron 55.0%
GGCCGGTCCAACATGTTTGG+AGG + chr2.2:33085842-33085861 MS.gene68821:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 33085226 33086811 33085226 ID=MS.gene68821
chr2.2 mRNA 33085226 33086811 33085226 ID=MS.gene68821.t1;Parent=MS.gene68821
chr2.2 exon 33085226 33085663 33085226 ID=MS.gene68821.t1.exon1;Parent=MS.gene68821.t1
chr2.2 CDS 33085226 33085663 33085226 ID=cds.MS.gene68821.t1;Parent=MS.gene68821.t1
chr2.2 exon 33086282 33086519 33086282 ID=MS.gene68821.t1.exon2;Parent=MS.gene68821.t1
chr2.2 CDS 33086282 33086519 33086282 ID=cds.MS.gene68821.t1;Parent=MS.gene68821.t1
chr2.2 exon 33086636 33086811 33086636 ID=MS.gene68821.t1.exon3;Parent=MS.gene68821.t1
chr2.2 CDS 33086636 33086811 33086636 ID=cds.MS.gene68821.t1;Parent=MS.gene68821.t1
Gene Sequence

>MS.gene68821

ATGGAAAAAAATGTTGCAATTGTTGGGGCAGGAATAAGTGGTATACTTGCCTGCAAATATGCTCTACAAATTGGTTTTCATCCAATTGTCTACGAATCTGACGATGGCATTGGTGGAATATGGAGACATACTATTCAGTCTACCAAACTCCAAACTAACAAACAAGACTTCCAGTTTACAGATTTTCCATGGGATTCTTCTATAAAAGAAGATTTTCCAAGTAATCAACAAGTGCAAGATTATCTTAACTCCTATGCTCAACATTTTTCCATCATTCCATACATAAGATTCAATTCCAAAGTCATTGATATTGATTATGTTGGGGAGTCTCATGAAGAAATGAAGTCATGGGAATTATGGAGTGGTAATGGAAGTCCATTTGGTTCTAAGGGAACTTGGCATCTAACCGTAGAAGATACTAAGAATTTCACCACCGAGGTATGTAAATATGAATTGTAATTTTGTGTGTAACTTCTTTTTCTTTTTCATTTTGGTTTATTTCATATTTTTTTCTTGTTTGGGCAAAATCTTTATGCTTACAATTTCTATTTTCACTGTCTCAATAAAAAAGAAAAAATCAAATTGCAGTGGTGGTTAGAACTATTCGGACTCACATGGCCGGTCCAACATGTTTGGAGGTTTAAGACAAATTTTATAATGGGACCTTATAAAATAAATGAAAAAAATCAATTTACATGCGACCTTCTTTCTAACCAACAAAGCTAGAGAAACATTTGTAGTTGGATTATCATATTACATATTTATAACTAAATTGAAATTTTTTGACACTTTTTTTATTCAAAATTTGAAGGTTTAAAACCCTAATTTGGATTGCTTGGCCCCAACGAACCCATAACACGACCTCCCTTTCATGTTTATTTGTGTAGTTGTTGGACGCCATTTTACCTTATCGAACACCATACTATACACGTGAATAAATTTTGTAGTTTGAAAATGATATAAATATTTAATTAGCTTAAGGAAAAAGTTCCCATAAAAAAAAAAAAAAGGAAAAGCAACAATACATTAAACTAGTTTGAATATTTGTAAATTCAGGTTCACAAGGCTAAGTTTGTCATTCTTTGCATTGGAAAGTATAGTGGCTTTCCAAATATTCCTAAGTTCCCCCTTGGAAAAGGCCCAGAAATCTTTAAAGGTAAAGTTATGCATTCTTTGGATTATTCTGCCTTAGACAACAAGACTGCAGCTGAAATGATAAAAAACAAGAGAGTTACAATAGTAGGCTCAGGGAAATCTGCTTTTGATATTGCTGCTGAATGTGCAAATGCAAATGGTAAGAATATATTTGTCCATAATTGTACAGTGTATAGAGAATTCGTTATTTATAGTTTTCAATTTTAAATAACTACTTCAATTTATTTTTTTAAAATCAATTTTTCACCTCTATAGGAGTCACTCATCCTTGCACAATTATCCAAAGAACTACACACTGGTTTCTTCCAGATTTTGACATTGCGGGCATTAATCTTGGATACTTATATTTCAACCGTTTTGCGGAGCTTTTAGTACACAAGCCTGGAGAACCATTCCTACTTAGCCTTGTGGCCACTTTACTTTCACCATTG
Protein sequence

>MS.gene68821.t1

MEKNVAIVGAGISGILACKYALQIGFHPIVYESDDGIGGIWRHTIQSTKLQTNKQDFQFTDFPWDSSIKEDFPSNQQVQDYLNSYAQHFSIIPYIRFNSKVIDIDYVGESHEEMKSWELWSGNGSPFGSKGTWHLTVEDTKNFTTEVHKAKFVILCIGKYSGFPNIPKFPLGKGPEIFKGKVMHSLDYSALDNKTAAEMIKNKRVTIVGSGKSAFDIAAECANANGVTHPCTIIQRTTHWFLPDFDIAGINLGYLYFNRFAELLVHKPGEPFLLSLVATLLSPL