Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049359.t1 | XP_003602486.1 | 99.7 | 352 | 1 | 0 | 1 | 352 | 1 | 352 | 8.70E-201 | 709.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049359.t1 | Q9SVA6 | 91.8 | 353 | 28 | 1 | 1 | 352 | 1 | 353 | 3.2e-188 | 659.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049359.t1 | G7JAA5 | 99.7 | 352 | 1 | 0 | 1 | 352 | 1 | 352 | 6.2e-201 | 709.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049359 | MS.gene06479 | 0.924761 | 4.57E-90 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene072150 | MS.gene049359 | PPI |
| MS.gene049359 | MS.gene74304 | PPI |
| MS.gene049359 | MS.gene30893 | PPI |
| MS.gene92847 | MS.gene049359 | PPI |
| MS.gene049359 | MS.gene46830 | PPI |
| MS.gene029882 | MS.gene049359 | PPI |
| MS.gene073797 | MS.gene049359 | PPI |
| MS.gene049359 | MS.gene36673 | PPI |
| MS.gene83732 | MS.gene049359 | PPI |
| MS.gene049359 | MS.gene36568 | PPI |
| MS.gene25152 | MS.gene049359 | PPI |
| MS.gene011134 | MS.gene049359 | PPI |
| MS.gene049359 | MS.gene30367 | PPI |
| MS.gene07894 | MS.gene049359 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049359.t1 | MTR_3g094880 | 99.716 | 352 | 1 | 0 | 1 | 352 | 1 | 352 | 0.0 | 725 |
| MS.gene049359.t1 | MTR_8g020210 | 57.143 | 350 | 147 | 2 | 4 | 351 | 3 | 351 | 3.19e-139 | 403 |
| MS.gene049359.t1 | MTR_2g019790 | 35.652 | 115 | 71 | 2 | 65 | 176 | 319 | 433 | 1.11e-12 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049359.t1 | AT4G39520 | 91.218 | 353 | 30 | 1 | 1 | 352 | 1 | 353 | 0.0 | 658 |
| MS.gene049359.t1 | AT1G17470 | 56.286 | 350 | 150 | 2 | 4 | 351 | 3 | 351 | 1.28e-144 | 417 |
| MS.gene049359.t1 | AT1G17470 | 56.286 | 350 | 150 | 2 | 4 | 351 | 3 | 351 | 1.28e-144 | 417 |
| MS.gene049359.t1 | AT1G72660 | 55.143 | 350 | 154 | 2 | 4 | 351 | 3 | 351 | 1.30e-141 | 409 |
| MS.gene049359.t1 | AT1G72660 | 55.143 | 350 | 154 | 2 | 4 | 351 | 3 | 351 | 1.30e-141 | 409 |
| MS.gene049359.t1 | AT1G72660 | 55.143 | 350 | 154 | 2 | 4 | 351 | 3 | 351 | 1.30e-141 | 409 |
| MS.gene049359.t1 | AT1G72660 | 52.222 | 270 | 127 | 1 | 84 | 351 | 2 | 271 | 4.18e-103 | 308 |
| MS.gene049359.t1 | AT5G18570 | 27.513 | 189 | 114 | 4 | 65 | 230 | 379 | 567 | 3.00e-13 | 72.0 |
Find 102 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAATGCAGATATTACTTTA+AGG | 0.195713 | 3.1:+75702806 | MS.gene049359:CDS |
| ATAAGACGCTTATGAGTTAT+TGG | 0.217930 | 3.1:-75701324 | None:intergenic |
| TCCTCTTCCCTTTCCATCCT+TGG | 0.232626 | 3.1:-75700503 | None:intergenic |
| AGCCACCAAACCTGACTTTC+AGG | 0.267365 | 3.1:+75702686 | MS.gene049359:CDS |
| TTCTTAGATGACAATATTAC+AGG | 0.277031 | 3.1:-75703497 | None:intergenic |
| CAAGGCTACCGCTCATCATT+TGG | 0.286238 | 3.1:+75697602 | MS.gene049359:CDS |
| AATGATGCAGTGCTCTGGTC+TGG | 0.288648 | 3.1:+75704102 | MS.gene049359:intron |
| ATGGGCTTCTGGATAAAGTT+TGG | 0.291273 | 3.1:+75703411 | MS.gene049359:CDS |
| GTGGAACCTTGATGGGCTTC+TGG | 0.293542 | 3.1:+75703400 | MS.gene049359:CDS |
| AAGGCTACCGCTCATCATTT+GGG | 0.320743 | 3.1:+75697603 | MS.gene049359:CDS |
| TAGTCAGGATTCATACCTTT+AGG | 0.323820 | 3.1:-75703467 | None:intergenic |
| ACTACACTAACTTGCATTCC+AGG | 0.339401 | 3.1:+75699498 | MS.gene049359:CDS |
| TTCCTCCTGAAAGTCAGGTT+TGG | 0.374686 | 3.1:-75702691 | None:intergenic |
| TTTCTTTCCTCCTGAAAGTC+AGG | 0.381939 | 3.1:-75702696 | None:intergenic |
| CTCCTGAAAGTCAGGTTTGG+TGG | 0.386899 | 3.1:-75702688 | None:intergenic |
| ATGATGCAGTGCTCTGGTCT+GGG | 0.389872 | 3.1:+75704103 | MS.gene049359:intron |
| TGGAAAGGGAAGAGGAAGGC+AGG | 0.392906 | 3.1:+75700510 | MS.gene049359:CDS |
| ATTACAGGGTCTTCGTAGTC+AGG | 0.396058 | 3.1:-75703482 | None:intergenic |
| TGGGCTTCTGGATAAAGTTT+GGG | 0.396911 | 3.1:+75703412 | MS.gene049359:CDS |
| TCAGATCACACTTGAGGAAC+TGG | 0.403962 | 3.1:+75702915 | MS.gene049359:CDS |
| TTGGCACCCTCAATAATTCC+AGG | 0.407131 | 3.1:-75700484 | None:intergenic |
| TCACGGTATCAAGATCTAGA+TGG | 0.410734 | 3.1:-75702756 | None:intergenic |
| ACTCCTTACACCTACAACTA+AGG | 0.413818 | 3.1:+75698274 | MS.gene049359:CDS |
| CCGCCACCTCCCTTAGTTGT+AGG | 0.416550 | 3.1:-75698284 | None:intergenic |
| CACCTGGAGTGGAACCTTGA+TGG | 0.417281 | 3.1:+75703392 | MS.gene049359:CDS |
| ACTTGAGGAACTGGAGATCT+TGG | 0.417927 | 3.1:+75702924 | MS.gene049359:CDS |
| ACCTGGAGTGGAACCTTGAT+GGG | 0.417928 | 3.1:+75703393 | MS.gene049359:CDS |
| TGCCCTCAATGACATCTATA+AGG | 0.420928 | 3.1:-75702849 | None:intergenic |
| AGCTAGAAGGATTTGGCATA+AGG | 0.430372 | 3.1:+75701355 | MS.gene049359:CDS |
| CTCGCTACATATAGCCTTCA+CGG | 0.438245 | 3.1:-75702773 | None:intergenic |
| TTCCTCATTACTGCCCTGTC+AGG | 0.441209 | 3.1:+75702953 | MS.gene049359:CDS |
| CTCCCTTAGTTGTAGGTGTA+AGG | 0.443291 | 3.1:-75698277 | None:intergenic |
| TTCCAAATGATGCAGTGCTC+TGG | 0.443660 | 3.1:+75704097 | MS.gene049359:intron |
| GCCCATCAAGGTTCCACTCC+AGG | 0.455391 | 3.1:-75703394 | None:intergenic |
| GACTTTCAGGAGGAAAGAAA+AGG | 0.462402 | 3.1:+75702699 | MS.gene049359:CDS |
| ACTAAGGGAGGTGGCGGTGC+TGG | 0.471973 | 3.1:+75698290 | MS.gene049359:CDS |
| GATCTCCCTGGAATTATTGA+GGG | 0.477457 | 3.1:+75700478 | MS.gene049359:CDS |
| TGTCAGTTGCTGGATCTCCC+TGG | 0.485451 | 3.1:+75700466 | MS.gene049359:intron |
| CTTGTTCACAACATAGATGC+AGG | 0.490496 | 3.1:-75702887 | None:intergenic |
| CGTATTTATACTAAACCTAA+AGG | 0.494629 | 3.1:+75703452 | MS.gene049359:CDS |
| GGAGGTGGCGGTGCTGGTGA+AGG | 0.497652 | 3.1:+75698296 | MS.gene049359:CDS |
| GGATCTCCCTGGAATTATTG+AGG | 0.497825 | 3.1:+75700477 | MS.gene049359:CDS |
| ACTCTGCTGAATAAATTGAC+TGG | 0.498625 | 3.1:+75698386 | MS.gene049359:CDS |
| CTCGTGGGCTTCCCGTCAGT+TGG | 0.504184 | 3.1:+75698356 | MS.gene049359:CDS |
| CTCTGCTGAATAAATTGACT+GGG | 0.511261 | 3.1:+75698387 | MS.gene049359:CDS |
| AGTTAGTGTAGTGAACTCAT+AGG | 0.513041 | 3.1:-75699487 | None:intergenic |
| CATCCTCTAGTATCTCATGC+AGG | 0.523981 | 3.1:-75704387 | None:intergenic |
| ATTATTGAGGGTGCCAAGGA+TGG | 0.524161 | 3.1:+75700490 | MS.gene049359:CDS |
| GCCAAGGATGGAAAGGGAAG+AGG | 0.527508 | 3.1:+75700502 | MS.gene049359:CDS |
| CCACGATATGTGATCACACC+TGG | 0.532149 | 3.1:-75699516 | None:intergenic |
| CTTTATCCAGAAGCCCATCA+AGG | 0.533921 | 3.1:-75703406 | None:intergenic |
| TCTGTTATTCAGTGCACACC+TGG | 0.544525 | 3.1:+75703376 | MS.gene049359:intron |
| TGGAATTATTGAGGGTGCCA+AGG | 0.547326 | 3.1:+75700486 | MS.gene049359:CDS |
| TGAGGGTGCCAAGGATGGAA+AGG | 0.549980 | 3.1:+75700495 | MS.gene049359:CDS |
| CACGGTATCAAGATCTAGAT+GGG | 0.550347 | 3.1:-75702755 | None:intergenic |
| GATGCAAGAGTCGGTCTCGT+GGG | 0.558118 | 3.1:+75698341 | MS.gene049359:CDS |
| TCATCATTTGGGGTTACTGA+AGG | 0.559084 | 3.1:+75697614 | MS.gene049359:CDS |
| CTCCTTACACCTACAACTAA+GGG | 0.561218 | 3.1:+75698275 | MS.gene049359:CDS |
| GGATGCAAGAGTCGGTCTCG+TGG | 0.561453 | 3.1:+75698340 | MS.gene049359:CDS |
| GAGGGTGCCAAGGATGGAAA+GGG | 0.568429 | 3.1:+75700496 | MS.gene049359:CDS |
| GAGTAGACTTGCCAACTGAC+GGG | 0.569689 | 3.1:-75698367 | None:intergenic |
| GACCTTATAGATGTCATTGA+GGG | 0.569803 | 3.1:+75702847 | MS.gene049359:CDS |
| AGAGTAGACTTGCCAACTGA+CGG | 0.570529 | 3.1:-75698368 | None:intergenic |
| TGATGCAGTGCTCTGGTCTG+GGG | 0.575620 | 3.1:+75704104 | MS.gene049359:intron |
| TGTTACAAGAATTACCTGAC+AGG | 0.582982 | 3.1:-75702967 | None:intergenic |
| CTAAACTTGCAAAACTGAGG+AGG | 0.585745 | 3.1:+75698249 | MS.gene049359:CDS |
| ATCCTCTAGTATCTCATGCA+GGG | 0.588051 | 3.1:-75704386 | None:intergenic |
| TACCTGACAGGGCAGTAATG+AGG | 0.589669 | 3.1:-75702955 | None:intergenic |
| ACTTTCAGGAGGAAAGAAAA+GGG | 0.590692 | 3.1:+75702700 | MS.gene049359:CDS |
| TCAGGAGGAAAGAAAAGGGT+GGG | 0.592993 | 3.1:+75702704 | MS.gene049359:CDS |
| TGACCTTATAGATGTCATTG+AGG | 0.593679 | 3.1:+75702846 | MS.gene049359:CDS |
| AATCAAAGACATCGAAGATG+AGG | 0.596340 | 3.1:+75697154 | MS.gene049359:CDS |
| ATCTATAAGGTCATCTGCAG+TGG | 0.597376 | 3.1:-75702836 | None:intergenic |
| TATTCAGTGCACACCTGGAG+TGG | 0.599235 | 3.1:+75703381 | MS.gene049359:intron |
| AGTATCTCATGCAGGGAATG+TGG | 0.603443 | 3.1:-75704379 | None:intergenic |
| TTTCTGCGATCGCATTCACA+AGG | 0.607440 | 3.1:+75703535 | MS.gene049359:CDS |
| TAAACTTGCAAAACTGAGGA+GGG | 0.608244 | 3.1:+75698250 | MS.gene049359:CDS |
| TCGTTCTTCAACAATGGCGA+CGG | 0.609637 | 3.1:+75697121 | None:intergenic |
| AGGCTACCGCTCATCATTTG+GGG | 0.610664 | 3.1:+75697604 | MS.gene049359:CDS |
| TCTTAGATGACAATATTACA+GGG | 0.622650 | 3.1:-75703496 | None:intergenic |
| ACACCTACAACTAAGGGAGG+TGG | 0.623374 | 3.1:+75698281 | MS.gene049359:CDS |
| CAAGCCTCAGAGAGTCGGCA+AGG | 0.623563 | 3.1:+75704142 | MS.gene049359:CDS |
| AAACACAAGCCTCAGAGAGT+CGG | 0.626180 | 3.1:+75704137 | MS.gene049359:CDS |
| TCTCATGCAGGGAATGTGGT+CGG | 0.637938 | 3.1:-75704375 | None:intergenic |
| GATTGATCAGATCACACTTG+AGG | 0.641269 | 3.1:+75702909 | MS.gene049359:CDS |
| CCAGGTGTGATCACATATCG+TGG | 0.641756 | 3.1:+75699516 | MS.gene049359:CDS |
| TGGCACCCTCAATAATTCCA+GGG | 0.644735 | 3.1:-75700483 | None:intergenic |
| AAAAGTGGGGATGCAAGAGT+CGG | 0.647820 | 3.1:+75698332 | MS.gene049359:CDS |
| CACAGGTTATTAGTACTGCA+AGG | 0.648199 | 3.1:+75701262 | MS.gene049359:intron |
| TCTAGATCTTGATACCGTGA+AGG | 0.652544 | 3.1:+75702759 | MS.gene049359:CDS |
| TTCAGGAGGAAAGAAAAGGG+TGG | 0.654637 | 3.1:+75702703 | MS.gene049359:CDS |
| TCTACCTTGCCGACTCTCTG+AGG | 0.654923 | 3.1:-75704146 | None:intergenic |
| GTTACAAGAATTACCTGACA+GGG | 0.655869 | 3.1:-75702966 | None:intergenic |
| CCTACAACTAAGGGAGGTGG+CGG | 0.656986 | 3.1:+75698284 | MS.gene049359:CDS |
| AGGCTAAACTTGCAAAACTG+AGG | 0.657417 | 3.1:+75698246 | MS.gene049359:intron |
| TTGTTCACAACATAGATGCA+GGG | 0.658113 | 3.1:-75702886 | None:intergenic |
| TTCCCTGCATGAGATACTAG+AGG | 0.664860 | 3.1:+75704384 | MS.gene049359:CDS |
| GGCGAAAACTCAAAAGAACA+AGG | 0.674392 | 3.1:+75697584 | MS.gene049359:CDS |
| CACCAAACCTGACTTTCAGG+AGG | 0.677415 | 3.1:+75702689 | MS.gene049359:CDS |
| AGGATGGAAAGGGAAGAGGA+AGG | 0.692562 | 3.1:+75700506 | MS.gene049359:CDS |
| CAGTAACCCCAAATGATGAG+CGG | 0.699908 | 3.1:-75697610 | None:intergenic |
| CTTACACCTACAACTAAGGG+AGG | 0.707070 | 3.1:+75698278 | MS.gene049359:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 75697134 | 75704414 | 75697134 | ID=MS.gene049359 |
| chr3.1 | mRNA | 75697134 | 75704414 | 75697134 | ID=MS.gene049359.t1;Parent=MS.gene049359 |
| chr3.1 | exon | 75697134 | 75697175 | 75697134 | ID=MS.gene049359.t1.exon1;Parent=MS.gene049359.t1 |
| chr3.1 | CDS | 75697134 | 75697175 | 75697134 | ID=cds.MS.gene049359.t1;Parent=MS.gene049359.t1 |
| chr3.1 | exon | 75697582 | 75697635 | 75697582 | ID=MS.gene049359.t1.exon2;Parent=MS.gene049359.t1 |
| chr3.1 | CDS | 75697582 | 75697635 | 75697582 | ID=cds.MS.gene049359.t1;Parent=MS.gene049359.t1 |
| chr3.1 | exon | 75698248 | 75698421 | 75698248 | ID=MS.gene049359.t1.exon3;Parent=MS.gene049359.t1 |
| chr3.1 | CDS | 75698248 | 75698421 | 75698248 | ID=cds.MS.gene049359.t1;Parent=MS.gene049359.t1 |
| chr3.1 | exon | 75699480 | 75699551 | 75699480 | ID=MS.gene049359.t1.exon4;Parent=MS.gene049359.t1 |
| chr3.1 | CDS | 75699480 | 75699551 | 75699480 | ID=cds.MS.gene049359.t1;Parent=MS.gene049359.t1 |
| chr3.1 | exon | 75700472 | 75700531 | 75700472 | ID=MS.gene049359.t1.exon5;Parent=MS.gene049359.t1 |
| chr3.1 | CDS | 75700472 | 75700531 | 75700472 | ID=cds.MS.gene049359.t1;Parent=MS.gene049359.t1 |
| chr3.1 | exon | 75701267 | 75701376 | 75701267 | ID=MS.gene049359.t1.exon6;Parent=MS.gene049359.t1 |
| chr3.1 | CDS | 75701267 | 75701376 | 75701267 | ID=cds.MS.gene049359.t1;Parent=MS.gene049359.t1 |
| chr3.1 | exon | 75702675 | 75702974 | 75702675 | ID=MS.gene049359.t1.exon7;Parent=MS.gene049359.t1 |
| chr3.1 | CDS | 75702675 | 75702974 | 75702675 | ID=cds.MS.gene049359.t1;Parent=MS.gene049359.t1 |
| chr3.1 | exon | 75703388 | 75703579 | 75703388 | ID=MS.gene049359.t1.exon8;Parent=MS.gene049359.t1 |
| chr3.1 | CDS | 75703388 | 75703579 | 75703388 | ID=cds.MS.gene049359.t1;Parent=MS.gene049359.t1 |
| chr3.1 | exon | 75704112 | 75704201 | 75704112 | ID=MS.gene049359.t1.exon9;Parent=MS.gene049359.t1 |
| chr3.1 | CDS | 75704112 | 75704201 | 75704112 | ID=cds.MS.gene049359.t1;Parent=MS.gene049359.t1 |
| chr3.1 | exon | 75704303 | 75704414 | 75704303 | ID=MS.gene049359.t1.exon10;Parent=MS.gene049359.t1 |
| chr3.1 | CDS | 75704303 | 75704414 | 75704303 | ID=cds.MS.gene049359.t1;Parent=MS.gene049359.t1 |
| Gene Sequence |
| Protein sequence |
