Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06479.t1 | XP_003602486.1 | 99.7 | 352 | 1 | 0 | 1 | 352 | 1 | 352 | 8.70E-201 | 709.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06479.t1 | Q9SVA6 | 91.8 | 353 | 28 | 1 | 1 | 352 | 1 | 353 | 3.2e-188 | 659.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06479.t1 | G7JAA5 | 99.7 | 352 | 1 | 0 | 1 | 352 | 1 | 352 | 6.2e-201 | 709.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049359 | MS.gene06479 | 0.924761 | 4.57E-90 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene072150 | MS.gene06479 | PPI |
| MS.gene25152 | MS.gene06479 | PPI |
| MS.gene06479 | MS.gene36568 | PPI |
| MS.gene011134 | MS.gene06479 | PPI |
| MS.gene06479 | MS.gene30367 | PPI |
| MS.gene07894 | MS.gene06479 | PPI |
| MS.gene06479 | MS.gene74304 | PPI |
| MS.gene06479 | MS.gene30893 | PPI |
| MS.gene029882 | MS.gene06479 | PPI |
| MS.gene83732 | MS.gene06479 | PPI |
| MS.gene92847 | MS.gene06479 | PPI |
| MS.gene073797 | MS.gene06479 | PPI |
| MS.gene06479 | MS.gene46830 | PPI |
| MS.gene06479 | MS.gene36673 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06479.t1 | MTR_3g094880 | 99.716 | 352 | 1 | 0 | 1 | 352 | 1 | 352 | 0.0 | 725 |
| MS.gene06479.t1 | MTR_8g020210 | 57.143 | 350 | 147 | 2 | 4 | 351 | 3 | 351 | 3.19e-139 | 403 |
| MS.gene06479.t1 | MTR_2g019790 | 35.652 | 115 | 71 | 2 | 65 | 176 | 319 | 433 | 1.11e-12 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06479.t1 | AT4G39520 | 91.218 | 353 | 30 | 1 | 1 | 352 | 1 | 353 | 0.0 | 658 |
| MS.gene06479.t1 | AT1G17470 | 56.286 | 350 | 150 | 2 | 4 | 351 | 3 | 351 | 1.28e-144 | 417 |
| MS.gene06479.t1 | AT1G17470 | 56.286 | 350 | 150 | 2 | 4 | 351 | 3 | 351 | 1.28e-144 | 417 |
| MS.gene06479.t1 | AT1G72660 | 55.143 | 350 | 154 | 2 | 4 | 351 | 3 | 351 | 1.30e-141 | 409 |
| MS.gene06479.t1 | AT1G72660 | 55.143 | 350 | 154 | 2 | 4 | 351 | 3 | 351 | 1.30e-141 | 409 |
| MS.gene06479.t1 | AT1G72660 | 55.143 | 350 | 154 | 2 | 4 | 351 | 3 | 351 | 1.30e-141 | 409 |
| MS.gene06479.t1 | AT1G72660 | 52.222 | 270 | 127 | 1 | 84 | 351 | 2 | 271 | 4.18e-103 | 308 |
| MS.gene06479.t1 | AT5G18570 | 27.513 | 189 | 114 | 4 | 65 | 230 | 379 | 567 | 3.00e-13 | 72.0 |
Find 102 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAATGCAGATATTACTTTA+AGG | 0.195713 | 3.2:+79346572 | MS.gene06479:CDS |
| ATAAGACGCTTATGAGTTAT+TGG | 0.217930 | 3.2:-79345086 | None:intergenic |
| TCCTCTTCCCTTTCCATCCT+TGG | 0.232626 | 3.2:-79344272 | None:intergenic |
| AGCCACCAAACCTGACTTTC+AGG | 0.267365 | 3.2:+79346452 | MS.gene06479:CDS |
| TTCTTAGATGACAATATTAC+AGG | 0.277031 | 3.2:-79347265 | None:intergenic |
| CAAGGCTACCGCTCATCATT+TGG | 0.286238 | 3.2:+79341364 | MS.gene06479:CDS |
| AATGATGCAGTGCTCTGGTC+TGG | 0.288648 | 3.2:+79347869 | MS.gene06479:intron |
| ATGGGCTTCTGGATAAAGTT+TGG | 0.291273 | 3.2:+79347179 | MS.gene06479:CDS |
| GTGGAACCTTGATGGGCTTC+TGG | 0.293542 | 3.2:+79347168 | MS.gene06479:CDS |
| AAGGCTACCGCTCATCATTT+GGG | 0.320743 | 3.2:+79341365 | MS.gene06479:CDS |
| TAGTCAGGATTCATACCTTT+AGG | 0.323820 | 3.2:-79347235 | None:intergenic |
| ACTACACTAACTTGCATTCC+AGG | 0.339401 | 3.2:+79343271 | MS.gene06479:CDS |
| TTCCTCCTGAAAGTCAGGTT+TGG | 0.374686 | 3.2:-79346457 | None:intergenic |
| TTTCTTTCCTCCTGAAAGTC+AGG | 0.381939 | 3.2:-79346462 | None:intergenic |
| CTCCTGAAAGTCAGGTTTGG+TGG | 0.386899 | 3.2:-79346454 | None:intergenic |
| ATGATGCAGTGCTCTGGTCT+GGG | 0.389872 | 3.2:+79347870 | MS.gene06479:intron |
| TGGAAAGGGAAGAGGAAGGC+AGG | 0.392906 | 3.2:+79344279 | MS.gene06479:CDS |
| ATTACAGGGTCTTCGTAGTC+AGG | 0.396058 | 3.2:-79347250 | None:intergenic |
| TGGGCTTCTGGATAAAGTTT+GGG | 0.396911 | 3.2:+79347180 | MS.gene06479:CDS |
| TCAGATCACACTTGAGGAAC+TGG | 0.403962 | 3.2:+79346681 | MS.gene06479:CDS |
| TTGGCACCCTCAATAATTCC+AGG | 0.407131 | 3.2:-79344253 | None:intergenic |
| TCACGGTATCAAGATCTAGA+TGG | 0.410734 | 3.2:-79346522 | None:intergenic |
| ACTCCTTACACCTACAACTA+AGG | 0.413818 | 3.2:+79342039 | MS.gene06479:CDS |
| CCGCCACCTCCCTTAGTTGT+AGG | 0.416550 | 3.2:-79342049 | None:intergenic |
| CACCTGGAGTGGAACCTTGA+TGG | 0.417281 | 3.2:+79347160 | MS.gene06479:CDS |
| ACTTGAGGAACTGGAGATCT+TGG | 0.417927 | 3.2:+79346690 | MS.gene06479:CDS |
| ACCTGGAGTGGAACCTTGAT+GGG | 0.417928 | 3.2:+79347161 | MS.gene06479:CDS |
| TGCCCTCAATGACATCTATA+AGG | 0.420928 | 3.2:-79346615 | None:intergenic |
| AGCTAGAAGGATTTGGCATA+AGG | 0.430372 | 3.2:+79345117 | MS.gene06479:CDS |
| CTCGCTACATATAGCCTTCA+CGG | 0.438245 | 3.2:-79346539 | None:intergenic |
| TTCCTCATTACTGCCCTGTC+AGG | 0.441209 | 3.2:+79346719 | MS.gene06479:CDS |
| CTCCCTTAGTTGTAGGTGTA+AGG | 0.443291 | 3.2:-79342042 | None:intergenic |
| TTCCAAATGATGCAGTGCTC+TGG | 0.443660 | 3.2:+79347864 | MS.gene06479:intron |
| GCCCATCAAGGTTCCACTCC+AGG | 0.455391 | 3.2:-79347162 | None:intergenic |
| GACTTTCAGGAGGAAAGAAA+AGG | 0.462402 | 3.2:+79346465 | MS.gene06479:CDS |
| ACTAAGGGAGGTGGCGGTGC+TGG | 0.471973 | 3.2:+79342055 | MS.gene06479:CDS |
| GATCTCCCTGGAATTATTGA+GGG | 0.477457 | 3.2:+79344247 | MS.gene06479:CDS |
| TGTCAGTTGCTGGATCTCCC+TGG | 0.485451 | 3.2:+79344235 | MS.gene06479:intron |
| CTTGTTCACAACATAGATGC+AGG | 0.490496 | 3.2:-79346653 | None:intergenic |
| CGTATTTATACTAAACCTAA+AGG | 0.494629 | 3.2:+79347220 | MS.gene06479:CDS |
| GGAGGTGGCGGTGCTGGTGA+AGG | 0.497652 | 3.2:+79342061 | MS.gene06479:CDS |
| GGATCTCCCTGGAATTATTG+AGG | 0.497825 | 3.2:+79344246 | MS.gene06479:CDS |
| ACTCTGCTGAATAAATTGAC+TGG | 0.498625 | 3.2:+79342151 | MS.gene06479:CDS |
| CTCGTGGGCTTCCCGTCAGT+TGG | 0.504184 | 3.2:+79342121 | MS.gene06479:CDS |
| CTCTGCTGAATAAATTGACT+GGG | 0.511261 | 3.2:+79342152 | MS.gene06479:CDS |
| AGTTAGTGTAGTGAACTCAT+AGG | 0.513041 | 3.2:-79343260 | None:intergenic |
| CATCCTCTAGTATCTCATGC+AGG | 0.523981 | 3.2:-79348154 | None:intergenic |
| ATTATTGAGGGTGCCAAGGA+TGG | 0.524161 | 3.2:+79344259 | MS.gene06479:CDS |
| GCCAAGGATGGAAAGGGAAG+AGG | 0.527508 | 3.2:+79344271 | MS.gene06479:CDS |
| CCACGATATGTGATCACACC+TGG | 0.532149 | 3.2:-79343289 | None:intergenic |
| CTTTATCCAGAAGCCCATCA+AGG | 0.533921 | 3.2:-79347174 | None:intergenic |
| TCTGTTATTCAGTGCACACC+TGG | 0.544525 | 3.2:+79347144 | MS.gene06479:intron |
| TGGAATTATTGAGGGTGCCA+AGG | 0.547326 | 3.2:+79344255 | MS.gene06479:CDS |
| TGAGGGTGCCAAGGATGGAA+AGG | 0.549980 | 3.2:+79344264 | MS.gene06479:CDS |
| CACGGTATCAAGATCTAGAT+GGG | 0.550347 | 3.2:-79346521 | None:intergenic |
| GATGCAAGAGTCGGTCTCGT+GGG | 0.558118 | 3.2:+79342106 | MS.gene06479:CDS |
| TCATCATTTGGGGTTACTGA+AGG | 0.559084 | 3.2:+79341376 | MS.gene06479:CDS |
| CTCCTTACACCTACAACTAA+GGG | 0.561218 | 3.2:+79342040 | MS.gene06479:CDS |
| GGATGCAAGAGTCGGTCTCG+TGG | 0.561453 | 3.2:+79342105 | MS.gene06479:CDS |
| GAGGGTGCCAAGGATGGAAA+GGG | 0.568429 | 3.2:+79344265 | MS.gene06479:CDS |
| GAGTAGACTTGCCAACTGAC+GGG | 0.569689 | 3.2:-79342132 | None:intergenic |
| GACCTTATAGATGTCATTGA+GGG | 0.569803 | 3.2:+79346613 | MS.gene06479:CDS |
| AGAGTAGACTTGCCAACTGA+CGG | 0.570529 | 3.2:-79342133 | None:intergenic |
| TGATGCAGTGCTCTGGTCTG+GGG | 0.575620 | 3.2:+79347871 | MS.gene06479:intron |
| TGTTACAAGAATTACCTGAC+AGG | 0.583571 | 3.2:-79346733 | None:intergenic |
| CTAAACTTGCAAAACTGAGG+AGG | 0.585745 | 3.2:+79342014 | MS.gene06479:CDS |
| ATCCTCTAGTATCTCATGCA+GGG | 0.588051 | 3.2:-79348153 | None:intergenic |
| TACCTGACAGGGCAGTAATG+AGG | 0.589669 | 3.2:-79346721 | None:intergenic |
| ACTTTCAGGAGGAAAGAAAA+GGG | 0.590692 | 3.2:+79346466 | MS.gene06479:CDS |
| TCAGGAGGAAAGAAAAGGGT+GGG | 0.592993 | 3.2:+79346470 | MS.gene06479:CDS |
| TGACCTTATAGATGTCATTG+AGG | 0.593679 | 3.2:+79346612 | MS.gene06479:CDS |
| AATCAAAGACATCGAAGATG+AGG | 0.596340 | 3.2:+79340913 | MS.gene06479:CDS |
| ATCTATAAGGTCATCTGCAG+TGG | 0.597376 | 3.2:-79346602 | None:intergenic |
| TATTCAGTGCACACCTGGAG+TGG | 0.599235 | 3.2:+79347149 | MS.gene06479:intron |
| AGTATCTCATGCAGGGAATG+TGG | 0.603443 | 3.2:-79348146 | None:intergenic |
| TTTCTGCGATCGCATTCACA+AGG | 0.607440 | 3.2:+79347303 | MS.gene06479:CDS |
| TAAACTTGCAAAACTGAGGA+GGG | 0.608244 | 3.2:+79342015 | MS.gene06479:CDS |
| TCGTTCTTCAACAATGGCGA+CGG | 0.609637 | 3.2:+79340880 | None:intergenic |
| AGGCTACCGCTCATCATTTG+GGG | 0.610664 | 3.2:+79341366 | MS.gene06479:CDS |
| TCTTAGATGACAATATTACA+GGG | 0.622650 | 3.2:-79347264 | None:intergenic |
| ACACCTACAACTAAGGGAGG+TGG | 0.623374 | 3.2:+79342046 | MS.gene06479:CDS |
| CAAGCCTCAGAGAGTCGGCA+AGG | 0.623563 | 3.2:+79347909 | MS.gene06479:CDS |
| AAACACAAGCCTCAGAGAGT+CGG | 0.626180 | 3.2:+79347904 | MS.gene06479:CDS |
| TCTCATGCAGGGAATGTGGT+CGG | 0.637938 | 3.2:-79348142 | None:intergenic |
| GATTGATCAGATCACACTTG+AGG | 0.641269 | 3.2:+79346675 | MS.gene06479:CDS |
| CCAGGTGTGATCACATATCG+TGG | 0.641756 | 3.2:+79343289 | MS.gene06479:CDS |
| TGGCACCCTCAATAATTCCA+GGG | 0.644735 | 3.2:-79344252 | None:intergenic |
| AAAAGTGGGGATGCAAGAGT+CGG | 0.647820 | 3.2:+79342097 | MS.gene06479:CDS |
| CACAGGTTATTAGTACTGCA+AGG | 0.648199 | 3.2:+79345024 | MS.gene06479:intron |
| TCTAGATCTTGATACCGTGA+AGG | 0.652544 | 3.2:+79346525 | MS.gene06479:CDS |
| TTCAGGAGGAAAGAAAAGGG+TGG | 0.654637 | 3.2:+79346469 | MS.gene06479:CDS |
| TCTACCTTGCCGACTCTCTG+AGG | 0.654923 | 3.2:-79347913 | None:intergenic |
| CCTACAACTAAGGGAGGTGG+CGG | 0.656986 | 3.2:+79342049 | MS.gene06479:CDS |
| AGGCTAAACTTGCAAAACTG+AGG | 0.657417 | 3.2:+79342011 | MS.gene06479:intron |
| TTGTTCACAACATAGATGCA+GGG | 0.658113 | 3.2:-79346652 | None:intergenic |
| TTCCCTGCATGAGATACTAG+AGG | 0.664860 | 3.2:+79348151 | MS.gene06479:CDS |
| GGCGAAAACTCAAAAGAACA+AGG | 0.674392 | 3.2:+79341346 | MS.gene06479:CDS |
| CACCAAACCTGACTTTCAGG+AGG | 0.677415 | 3.2:+79346455 | MS.gene06479:CDS |
| AGGATGGAAAGGGAAGAGGA+AGG | 0.692562 | 3.2:+79344275 | MS.gene06479:CDS |
| CAGTAACCCCAAATGATGAG+CGG | 0.699908 | 3.2:-79341372 | None:intergenic |
| GTTACAAGAATTACCTGACA+GGG | 0.706732 | 3.2:-79346732 | None:intergenic |
| CTTACACCTACAACTAAGGG+AGG | 0.707070 | 3.2:+79342043 | MS.gene06479:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 79340893 | 79348181 | 79340893 | ID=MS.gene06479 |
| chr3.2 | mRNA | 79340893 | 79348181 | 79340893 | ID=MS.gene06479.t1;Parent=MS.gene06479 |
| chr3.2 | exon | 79340893 | 79340934 | 79340893 | ID=MS.gene06479.t1.exon1;Parent=MS.gene06479.t1 |
| chr3.2 | CDS | 79340893 | 79340934 | 79340893 | ID=cds.MS.gene06479.t1;Parent=MS.gene06479.t1 |
| chr3.2 | exon | 79341344 | 79341397 | 79341344 | ID=MS.gene06479.t1.exon2;Parent=MS.gene06479.t1 |
| chr3.2 | CDS | 79341344 | 79341397 | 79341344 | ID=cds.MS.gene06479.t1;Parent=MS.gene06479.t1 |
| chr3.2 | exon | 79342013 | 79342186 | 79342013 | ID=MS.gene06479.t1.exon3;Parent=MS.gene06479.t1 |
| chr3.2 | CDS | 79342013 | 79342186 | 79342013 | ID=cds.MS.gene06479.t1;Parent=MS.gene06479.t1 |
| chr3.2 | exon | 79343253 | 79343324 | 79343253 | ID=MS.gene06479.t1.exon4;Parent=MS.gene06479.t1 |
| chr3.2 | CDS | 79343253 | 79343324 | 79343253 | ID=cds.MS.gene06479.t1;Parent=MS.gene06479.t1 |
| chr3.2 | exon | 79344241 | 79344300 | 79344241 | ID=MS.gene06479.t1.exon5;Parent=MS.gene06479.t1 |
| chr3.2 | CDS | 79344241 | 79344300 | 79344241 | ID=cds.MS.gene06479.t1;Parent=MS.gene06479.t1 |
| chr3.2 | exon | 79345029 | 79345138 | 79345029 | ID=MS.gene06479.t1.exon6;Parent=MS.gene06479.t1 |
| chr3.2 | CDS | 79345029 | 79345138 | 79345029 | ID=cds.MS.gene06479.t1;Parent=MS.gene06479.t1 |
| chr3.2 | exon | 79346441 | 79346740 | 79346441 | ID=MS.gene06479.t1.exon7;Parent=MS.gene06479.t1 |
| chr3.2 | CDS | 79346441 | 79346740 | 79346441 | ID=cds.MS.gene06479.t1;Parent=MS.gene06479.t1 |
| chr3.2 | exon | 79347156 | 79347347 | 79347156 | ID=MS.gene06479.t1.exon8;Parent=MS.gene06479.t1 |
| chr3.2 | CDS | 79347156 | 79347347 | 79347156 | ID=cds.MS.gene06479.t1;Parent=MS.gene06479.t1 |
| chr3.2 | exon | 79347879 | 79347968 | 79347879 | ID=MS.gene06479.t1.exon9;Parent=MS.gene06479.t1 |
| chr3.2 | CDS | 79347879 | 79347968 | 79347879 | ID=cds.MS.gene06479.t1;Parent=MS.gene06479.t1 |
| chr3.2 | exon | 79348070 | 79348181 | 79348070 | ID=MS.gene06479.t1.exon10;Parent=MS.gene06479.t1 |
| chr3.2 | CDS | 79348070 | 79348181 | 79348070 | ID=cds.MS.gene06479.t1;Parent=MS.gene06479.t1 |
| Gene Sequence |
| Protein sequence |
