AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31282

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31282.t1 MTR_2g094570 97.711 699 9 1 1 692 1 699 0.0 1415
MS.gene31282.t1 MTR_2g094570 97.711 699 9 1 1 692 57 755 0.0 1415
MS.gene31282.t1 MTR_2g094570 97.243 653 11 1 1 646 1 653 0.0 1316
MS.gene31282.t1 MTR_4g058930 70.278 720 164 17 1 692 1 698 0.0 927
MS.gene31282.t1 MTR_4g058930 70.418 693 155 17 1 665 1 671 0.0 892
MS.gene31282.t1 MTR_1g064430 57.593 698 228 16 2 659 31 700 0.0 753
MS.gene31282.t1 MTR_1g064430 57.593 698 228 16 2 659 18 687 0.0 752
MS.gene31282.t1 MTR_1g094960 58.259 563 201 8 2 533 10 569 0.0 604
MS.gene31282.t1 MTR_7g101280 53.285 548 212 11 1 518 21 554 0.0 556
MS.gene31282.t1 MTR_0041s0110 53.285 548 212 11 1 518 21 554 0.0 556
MS.gene31282.t1 MTR_3g073420 44.134 537 268 12 7 524 15 538 9.80e-125 383
MS.gene31282.t1 MTR_7g101275 51.944 360 126 5 20 372 1 320 2.19e-121 368
MS.gene31282.t1 MTR_0041s0120 50.402 373 124 8 20 372 1 332 6.57e-116 354
MS.gene31282.t1 MTR_5g061220 40.289 484 232 14 11 475 19 464 8.20e-100 317
MS.gene31282.t1 MTR_2g024430 41.500 400 165 11 8 392 17 362 2.15e-91 294
MS.gene31282.t1 MTR_5g040880 37.223 497 232 15 1 471 1 443 1.63e-88 286
MS.gene31282.t1 MTR_5g082140 38.696 460 216 12 6 452 7 413 7.94e-87 280
MS.gene31282.t1 MTR_4g124900 40.250 400 198 9 6 399 18 382 5.10e-85 290
MS.gene31282.t1 MTR_4g124900 40.250 400 198 9 6 399 18 382 5.66e-85 290
MS.gene31282.t1 MTR_4g088210 41.885 382 169 9 4 368 31 376 2.45e-83 278
MS.gene31282.t1 MTR_4g088210 41.885 382 169 9 4 368 31 376 3.53e-83 278
MS.gene31282.t1 MTR_2g014770 39.900 401 186 9 11 391 53 418 4.28e-83 275
MS.gene31282.t1 MTR_5g076270 41.353 399 189 9 5 394 18 380 4.73e-83 280
MS.gene31282.t1 MTR_2g014770 39.651 401 187 9 11 391 53 418 1.98e-82 275
MS.gene31282.t1 MTR_8g100050 40.561 392 194 7 2 387 50 408 2.46e-82 276
MS.gene31282.t1 MTR_2g093740 41.687 403 187 11 11 397 56 426 5.74e-82 274
MS.gene31282.t1 MTR_2g018690 40.247 405 198 9 2 397 13 382 6.59e-82 278
MS.gene31282.t1 MTR_8g100050 40.561 392 194 7 2 387 50 408 1.33e-81 276
MS.gene31282.t1 MTR_2g093740 41.687 403 187 11 11 397 56 426 2.18e-81 274
MS.gene31282.t1 MTR_2g093740 41.687 403 187 11 11 397 56 426 5.40e-81 274
MS.gene31282.t1 MTR_3g064050 40.602 399 192 9 5 394 20 382 1.22e-80 274
MS.gene31282.t1 MTR_8g079492 39.604 404 201 9 2 397 13 381 2.31e-80 274
MS.gene31282.t1 MTR_2g043250 40.701 371 183 7 6 370 18 357 2.31e-80 277
MS.gene31282.t1 MTR_8g079492 39.604 404 201 9 2 397 13 381 2.33e-80 274
MS.gene31282.t1 MTR_2g006270 38.060 402 192 12 1 392 3 357 4.37e-80 262
MS.gene31282.t1 MTR_1g058210 35.566 433 225 11 6 427 7 396 2.29e-79 260
MS.gene31282.t1 MTR_7g062540 29.817 711 390 21 5 662 8 662 1.77e-78 264
MS.gene31282.t1 MTR_2g093740 40.385 416 187 12 11 397 56 439 4.09e-78 264
MS.gene31282.t1 MTR_4g060460 41.067 375 172 8 11 368 54 396 8.08e-78 265
MS.gene31282.t1 MTR_4g060460 41.067 375 172 8 11 368 54 396 1.04e-77 265
MS.gene31282.t1 MTR_2g093740 40.385 416 187 12 11 397 56 439 1.69e-77 263
MS.gene31282.t1 MTR_2g093740 40.385 416 187 12 11 397 56 439 2.82e-77 264
MS.gene31282.t1 MTR_4g021580 40.816 392 184 11 9 389 17 371 6.27e-77 259
MS.gene31282.t1 MTR_4g021580 40.816 392 184 11 9 389 17 371 7.16e-77 259
MS.gene31282.t1 MTR_1g024025 41.398 372 176 8 6 368 38 376 3.63e-76 266
MS.gene31282.t1 MTR_5g040740 40.822 365 153 10 8 358 9 324 3.94e-76 250
MS.gene31282.t1 MTR_8g101360 40.860 372 181 6 8 371 28 368 1.96e-75 263
MS.gene31282.t1 MTR_8g027440 32.249 676 359 21 11 655 29 636 2.16e-75 256
MS.gene31282.t1 MTR_2g005240 41.379 377 172 9 7 371 19 358 2.37e-75 256
MS.gene31282.t1 MTR_2g006380 35.829 374 177 11 8 369 17 339 1.07e-67 229
MS.gene31282.t1 MTR_8g079492 40.663 332 160 7 68 397 3 299 4.77e-63 225
MS.gene31282.t1 MTR_2g093740 41.748 309 149 6 89 397 19 296 3.32e-59 211
MS.gene31282.t1 MTR_2g093740 41.748 309 149 6 89 397 19 296 6.19e-59 211
MS.gene31282.t1 MTR_5g060780 36.022 372 184 12 8 369 9 336 1.55e-57 200
MS.gene31282.t1 MTR_5g061890 35.657 373 184 12 8 369 9 336 3.86e-57 199
MS.gene31282.t1 MTR_5g460920 35.753 372 185 12 8 369 9 336 2.79e-56 197
MS.gene31282.t1 MTR_2g093740 40.062 322 149 7 89 397 19 309 1.05e-55 201
MS.gene31282.t1 MTR_2g093740 40.062 322 149 7 89 397 19 309 2.27e-55 201
MS.gene31282.t1 MTR_5g060630 33.251 406 188 14 6 369 7 371 5.31e-55 197
MS.gene31282.t1 MTR_5g460740 33.251 406 188 14 6 369 7 371 5.31e-55 197
MS.gene31282.t1 MTR_5g062970 40.777 206 101 2 11 204 38 234 3.90e-49 172
MS.gene31282.t1 MTR_8g446900 31.232 349 192 6 50 398 5 305 5.34e-49 176
MS.gene31282.t1 MTR_5g062960 34.197 193 96 6 263 451 2 167 6.87e-22 94.7
MS.gene31282.t1 MTR_3g064050 40.157 127 68 4 270 394 25 145 5.84e-17 85.1
MS.gene31282.t1 MTR_5g060770 32.886 149 88 4 56 202 6 144 1.85e-16 82.0
MS.gene31282.t1 MTR_5g060770 31.163 215 112 11 243 443 122 314 3.59e-11 65.9
MS.gene31282.t1 MTR_5g032260 37.500 104 63 2 263 365 50 152 3.48e-13 72.4
MS.gene31282.t1 MTR_8g023990 38.614 101 60 2 103 203 167 265 4.09e-12 69.7
MS.gene31282.t1 MTR_1g058350 35.659 129 79 3 84 208 51 179 7.41e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31282.t1 AT2G28350 58.985 729 223 19 3 692 2 693 0.0 769
MS.gene31282.t1 AT4G30080 57.518 685 233 15 5 662 13 666 0.0 739
MS.gene31282.t1 AT2G28350 58.890 703 213 19 3 666 2 667 0.0 737
MS.gene31282.t1 AT1G77850 38.584 565 297 16 5 542 13 554 9.47e-113 352
MS.gene31282.t1 AT1G77850 38.584 565 297 16 5 542 13 554 1.24e-112 352
MS.gene31282.t1 AT5G37020 41.979 374 178 6 3 368 14 356 1.19e-84 283
MS.gene31282.t1 AT5G37020 41.979 374 178 6 3 368 14 356 2.25e-84 283
MS.gene31282.t1 AT1G59750 33.135 673 349 20 11 655 22 621 4.03e-83 276
MS.gene31282.t1 AT1G59750 33.135 673 349 20 11 655 22 621 4.03e-83 276
MS.gene31282.t1 AT1G59750 33.036 672 348 20 11 655 22 618 6.01e-83 276
MS.gene31282.t1 AT5G60450 40.796 402 187 8 2 387 32 398 8.01e-82 275
MS.gene31282.t1 AT1G19220 39.152 401 203 9 5 399 17 382 3.50e-81 279
MS.gene31282.t1 AT5G60450 41.221 393 180 8 11 387 65 422 3.81e-81 274
MS.gene31282.t1 AT2G33860 38.806 402 197 10 11 398 54 420 2.62e-80 267
MS.gene31282.t1 AT5G62000 39.900 401 186 9 4 387 48 410 1.45e-79 271
MS.gene31282.t1 AT1G30330 40.347 404 197 9 3 397 14 382 1.60e-79 272
MS.gene31282.t1 AT5G62000 39.900 401 186 9 4 387 48 410 1.63e-79 271
MS.gene31282.t1 AT5G62000 39.900 401 186 9 4 387 48 410 1.63e-79 271
MS.gene31282.t1 AT5G62000 39.900 401 186 9 4 387 48 410 1.63e-79 271
MS.gene31282.t1 AT5G62000 39.900 401 186 9 4 387 48 410 1.63e-79 271
MS.gene31282.t1 AT1G30330 39.900 401 198 8 5 397 19 384 1.77e-79 272
MS.gene31282.t1 AT1G30330 39.900 401 198 8 5 397 19 384 1.77e-79 272
MS.gene31282.t1 AT5G20730 38.750 400 204 9 6 399 19 383 7.40e-79 273
MS.gene31282.t1 AT4G23980 31.587 668 356 19 11 655 12 601 2.21e-78 263
MS.gene31282.t1 AT5G20730 38.653 401 204 10 6 399 19 384 1.67e-77 270
MS.gene31282.t1 AT5G20730 38.653 401 204 10 6 399 19 384 1.88e-77 269
MS.gene31282.t1 AT1G59750 40.355 394 193 8 11 397 22 380 5.04e-76 257
MS.gene31282.t1 AT1G19850 40.270 370 180 8 8 368 51 388 1.28e-75 261
MS.gene31282.t1 AT1G19850 39.683 378 187 8 8 376 40 385 1.43e-75 261
MS.gene31282.t1 AT4G23980 40.431 371 176 9 11 372 12 346 2.95e-73 249
MS.gene31282.t1 AT2G46530 39.119 386 170 11 11 383 21 354 4.71e-72 245
MS.gene31282.t1 AT2G46530 39.119 386 170 11 11 383 42 375 7.47e-72 245
MS.gene31282.t1 AT3G61830 38.798 366 180 9 11 368 25 354 1.93e-68 235
MS.gene31282.t1 AT1G34410 29.198 661 354 19 11 655 27 589 7.90e-67 231
MS.gene31282.t1 AT1G34310 28.896 661 356 19 11 655 27 589 1.65e-64 224
MS.gene31282.t1 AT1G35240 29.198 661 340 21 11 655 27 575 2.16e-63 221
MS.gene31282.t1 AT1G34390 28.293 668 363 21 11 662 27 594 8.73e-63 220
MS.gene31282.t1 AT1G35520 29.085 667 347 21 11 655 27 589 7.36e-62 217
MS.gene31282.t1 AT5G37020 44.238 269 117 5 102 368 24 261 1.95e-59 213
MS.gene31282.t1 AT1G35540 36.458 384 180 11 11 382 27 358 1.08e-57 206
MS.gene31282.t1 AT2G33860 40.476 294 140 6 108 398 2 263 2.48e-57 201
MS.gene31282.t1 AT3G61830 39.062 320 157 8 51 368 20 303 3.08e-56 201
MS.gene31282.t1 AT1G34170 34.908 381 192 13 11 382 25 358 6.58e-55 196
MS.gene31282.t1 AT2G46530 38.849 278 123 7 107 383 36 267 2.19e-48 178
MS.gene31282.t1 AT1G34170 33.071 381 173 13 11 382 25 332 6.78e-48 176
MS.gene31282.t1 AT1G34170 33.508 382 170 14 11 382 25 332 6.57e-47 175
MS.gene31282.t1 AT1G43950 41.176 204 105 5 11 207 27 222 4.38e-40 147
MS.gene31282.t1 AT2G36080 34.043 141 85 4 103 238 33 170 1.85e-12 68.2
MS.gene31282.t1 AT1G13260 35.714 126 73 4 84 205 166 287 1.92e-12 69.7
MS.gene31282.t1 AT2G36080 34.043 141 85 4 103 238 33 170 2.27e-12 68.2
MS.gene31282.t1 AT2G36080 37.838 111 63 3 103 208 33 142 2.55e-12 66.2
MS.gene31282.t1 AT2G36080 37.838 111 63 3 103 208 33 142 2.55e-12 66.2
MS.gene31282.t1 AT1G01030 35.455 110 65 2 103 207 51 159 1.60e-11 66.6
MS.gene31282.t1 AT3G25730 35.294 102 62 2 108 205 183 284 1.75e-11 66.6
MS.gene31282.t1 AT1G01030 35.455 110 65 2 103 207 51 159 2.25e-11 66.6
MS.gene31282.t1 AT2G46870 35.965 114 67 3 100 208 27 139 2.45e-11 65.9
MS.gene31282.t1 AT4G01500 38.462 91 51 2 108 197 36 122 2.54e-11 66.2

Find 143 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAATCGTCTCTCGATTATT+TGG 0.140943 2.4:-7904354 None:intergenic
GAAAATACTTCATCAGTTTC+AGG 0.152637 2.4:-7902418 None:intergenic
GCACAATCCAACGTTGATTT+TGG 0.154632 2.4:+7902331 MS.gene31282:CDS
CGCTCCTGCCGCAGGCATTC+AGG 0.178149 2.4:+7904254 MS.gene31282:CDS
ATACAACACAAAGGGTTAAA+AGG 0.216528 2.4:-7904210 None:intergenic
TTGAAACTGAAGATTCTTCT+AGG 0.237698 2.4:+7903145 MS.gene31282:CDS
GGGAACTATTTCTTCTGTTC+AGG 0.264499 2.4:+7903182 MS.gene31282:CDS
TTCAAGCTCTGAATTTCTTA+AGG 0.295501 2.4:-7902458 None:intergenic
TTGACAATTCTGATGGATTC+AGG 0.309644 2.4:+7905044 MS.gene31282:CDS
TTCCCCTTGGAGACTTCTTC+AGG 0.314727 2.4:+7903230 MS.gene31282:CDS
TTCACATTGTGTAGTAAATC+TGG 0.325303 2.4:-7904033 None:intergenic
AGGATGTTCACGGTGAGATT+TGG 0.326034 2.4:+7902662 MS.gene31282:CDS
AGAAGTCTCCAAGGGGAATT+AGG 0.326359 2.4:-7903225 None:intergenic
TTGAGAGAGGAGAATAAGTT+GGG 0.327890 2.4:+7902937 MS.gene31282:CDS
AATTGTGGTCTTGGACCTTA+TGG 0.329009 2.4:+7902895 MS.gene31282:CDS
AGAAAACTGGCTAAGATGTT+TGG 0.333734 2.4:+7904801 MS.gene31282:CDS
ATGAGAATTCAATGGTGTTC+TGG 0.334279 2.4:+7903102 MS.gene31282:CDS
TGAAGAACTTTATAGAAAAC+TGG 0.335874 2.4:+7904788 MS.gene31282:CDS
ACACCTTCTGGATGGAGTTC+TGG 0.337274 2.4:+7902865 MS.gene31282:CDS
TTTGAGAGAGGAGAATAAGT+TGG 0.338801 2.4:+7902936 MS.gene31282:CDS
GCGAAGAGAGGGATTGTTAA+TGG 0.339338 2.4:+7902835 MS.gene31282:CDS
GGAGCCAGGCATGCTCAAAT+TGG 0.343033 2.4:+7904276 MS.gene31282:CDS
TCAAGCTCTGAATTTCTTAA+GGG 0.365482 2.4:-7902457 None:intergenic
TTCTAGGATTAGTTGGTTCA+TGG 0.372148 2.4:+7903161 MS.gene31282:CDS
AATGGATTGGAAACACCTTC+TGG 0.376276 2.4:+7902853 MS.gene31282:CDS
AAGATTCTTCTAGGATTAGT+TGG 0.376904 2.4:+7903154 MS.gene31282:CDS
TCAAATTGGAATATCTTTAT+CGG 0.377207 2.4:+7904290 MS.gene31282:CDS
TTTCTTTGCTCTTATTATCA+TGG 0.379754 2.4:-7904401 None:intergenic
ACTGAAATTTGTTGTTGTTA+AGG 0.379784 2.4:-7904320 None:intergenic
AAAATGTTGGTATTTGAAAC+TGG 0.386775 2.4:-7904179 None:intergenic
TTTGTGTTGTATATCTTCTT+CGG 0.389379 2.4:+7904221 MS.gene31282:CDS
AAATTCTCACAAGAGAAGTT+TGG 0.389679 2.4:+7904449 MS.gene31282:CDS
GTACTCTATCGCGATGCAAC+AGG 0.393430 2.4:+7904852 MS.gene31282:CDS
TGCTAGTAATCAAACATTTG+AGG 0.416661 2.4:+7903014 MS.gene31282:CDS
ACGGTGAGATTTGGAAGTTT+AGG 0.419091 2.4:+7902671 MS.gene31282:CDS
GCTCCTGCCGCAGGCATTCA+GGG 0.419621 2.4:+7904255 MS.gene31282:CDS
AGGAGGCATTTGTTAACAAC+TGG 0.420761 2.4:+7902715 MS.gene31282:CDS
AAGTCCATGGTTGGTCGAAT+TGG 0.421393 2.4:+7904062 MS.gene31282:CDS
GCTCCCTGAATGCCTGCGGC+AGG 0.423621 2.4:-7904258 None:intergenic
ATATCATGTCTGTTGACTAT+AGG 0.423697 2.4:+7904426 MS.gene31282:CDS
ATTAGATACTGGTCATTGTA+AGG 0.424326 2.4:+7904707 MS.gene31282:CDS
GGTAATGGAAATTGTGGTCT+TGG 0.431909 2.4:+7902886 MS.gene31282:CDS
ATTTGCAGGGTTTGTATCAC+AGG 0.434573 2.4:+7905198 MS.gene31282:intron
GAGAGGGATTGTTAATGGAT+TGG 0.434835 2.4:+7902840 MS.gene31282:CDS
TGGCAACTTGGATTAGATAC+TGG 0.435835 2.4:+7904696 MS.gene31282:CDS
AACAACAACAAATTTCAGTT+AGG 0.435844 2.4:+7904324 MS.gene31282:CDS
TTCAATGGTGTTCTGGGATG+AGG 0.437710 2.4:+7903109 MS.gene31282:CDS
ACCAACACAAGACAAGTCTA+AGG 0.439710 2.4:-7904760 None:intergenic
AAAATACTTCATCAGTTTCA+GGG 0.442229 2.4:-7902417 None:intergenic
TGAGAATTCAATGGTGTTCT+GGG 0.446818 2.4:+7903103 MS.gene31282:CDS
TTCTTCCTTGGTGGTGAGAA+GGG 0.447097 2.4:-7904114 None:intergenic
TTTCTTCCTTGGTGGTGAGA+AGG 0.447588 2.4:-7904115 None:intergenic
CCGCAGGCATTCAGGGAGCC+AGG 0.455283 2.4:+7904262 MS.gene31282:CDS
TTCGTTGGCCTAATTCCCCT+TGG 0.456414 2.4:+7903217 MS.gene31282:CDS
AAAGCTTAGATCCACAACTA+TGG 0.458962 2.4:+7902227 MS.gene31282:CDS
ACCTTAGACTTGTCTTGTGT+TGG 0.459246 2.4:+7904759 MS.gene31282:CDS
CAACTATGGCACGCATGTGC+TGG 0.459997 2.4:+7902241 MS.gene31282:CDS
TGTGTTGTTGTCCAAGAAAA+AGG 0.460383 2.4:+7904572 MS.gene31282:CDS
TAACACAACTGCAGGTCACT+TGG 0.460425 2.4:+7904004 MS.gene31282:intron
ATTTGTTGCTCAGTGAGAAT+TGG 0.462091 2.4:-7904525 None:intergenic
TTCGCAATCACCGTCTGAAC+CGG 0.462939 2.4:-7902640 None:intergenic
GAAGAATCTTCAGTTTCAAA+TGG 0.465560 2.4:-7903141 None:intergenic
TTTGCTCTTATTATCATGGT+TGG 0.466889 2.4:-7904397 None:intergenic
TACAACACAAAGGGTTAAAA+GGG 0.478847 2.4:-7904209 None:intergenic
GTTGTTAACAAATGCCTCCT+TGG 0.484302 2.4:-7902712 None:intergenic
TATTCCAATTTGAGCATGCC+TGG 0.486937 2.4:-7904280 None:intergenic
CAGGCAGCAAAGATACTAGA+AGG 0.487275 2.4:+7905063 MS.gene31282:CDS
TCTAGGATTAGTTGGTTCAT+GGG 0.488794 2.4:+7903162 MS.gene31282:CDS
ACAGGTGCTGTTAAACAAAC+TGG 0.490968 2.4:+7904870 MS.gene31282:CDS
AATTATAACGCTCCTGCCGC+AGG 0.493589 2.4:+7904246 MS.gene31282:CDS
GGGGAATTAGGCCAACGAAT+AGG 0.495789 2.4:-7903213 None:intergenic
AAAGTCTTTGCAAAAGATGC+AGG 0.501554 2.4:-7902523 None:intergenic
AAGTTTAGGCATATCTATAG+AGG 0.501773 2.4:+7902685 MS.gene31282:CDS
AATCCGGATGTTGTGGAAAA+CGG 0.502333 2.4:-7904140 None:intergenic
ACAACATCGAGAGGGAAATC+CGG 0.506711 2.4:-7904156 None:intergenic
AAACGGAGTTTCTTCCTTGG+TGG 0.510040 2.4:-7904123 None:intergenic
TGATTACTCGGCTGAGCCAC+CGG 0.514373 2.4:+7902621 MS.gene31282:CDS
TCTTAAACAAAGATGCTCAT+AGG 0.516080 2.4:-7905267 None:intergenic
GCATGTTCTGCATGCCCTTG+TGG 0.516128 2.4:-7902310 None:intergenic
GGCATTTGTTAACAACTGGT+TGG 0.516900 2.4:+7902719 MS.gene31282:CDS
GGAAAACGGAGTTTCTTCCT+TGG 0.523736 2.4:-7904126 None:intergenic
ATCGTTTCGGCACAGTATCT+AGG 0.523934 2.4:-7902586 None:intergenic
TCTGGATGGAGTTCTGGTAA+TGG 0.531132 2.4:+7902871 MS.gene31282:CDS
AAGAAGATATACAACACAAA+GGG 0.535342 2.4:-7904218 None:intergenic
AGAGGGAAATCCGGATGTTG+TGG 0.540838 2.4:-7904147 None:intergenic
AAACTGGTGAAGAACCCTTC+AGG 0.541832 2.4:+7904886 MS.gene31282:CDS
GTATTCGTCGCGCGAAGAGA+GGG 0.550772 2.4:+7902824 MS.gene31282:CDS
AGGTTGTTGATCCTATTCGT+TGG 0.555272 2.4:+7903202 MS.gene31282:CDS
GGCTGAGCCACCGGTTCAGA+CGG 0.555712 2.4:+7902630 MS.gene31282:CDS
TTAGATGCATCAAATAAGAC+AGG 0.558939 2.4:+7905243 MS.gene31282:CDS
AAAGGGTTGCCTGAAAATGT+TGG 0.562489 2.4:-7904192 None:intergenic
GCACATGCGTGCCATAGTTG+TGG 0.562709 2.4:-7902238 None:intergenic
TCTGATGCAAACAATGGTGG+AGG 0.563598 2.4:+7902553 MS.gene31282:CDS
GATACCAATTCGACCAACCA+TGG 0.567114 2.4:-7904066 None:intergenic
AACACAACTGCAGGTCACTT+GGG 0.568086 2.4:+7904005 MS.gene31282:intron
CTATGGCACGCATGTGCTGG+AGG 0.570767 2.4:+7902244 MS.gene31282:CDS
GGGTTTGTATCACAGGAACT+AGG 0.573351 2.4:+7905205 MS.gene31282:intron
GTGTTTCTGAGAGGTGAAAG+TGG 0.574136 2.4:+7902787 MS.gene31282:CDS
GATTGGAAACACCTTCTGGA+TGG 0.576113 2.4:+7902857 MS.gene31282:CDS
TGAAACTGGACAACATCGAG+AGG 0.579890 2.4:-7904165 None:intergenic
GCAATCACCGTCTGAACCGG+TGG 0.583092 2.4:-7902637 None:intergenic
ACCACTCATCCTCTGTAGAG+TGG 0.584023 2.4:+7902372 MS.gene31282:CDS
TGAGAGAGGAGAATAAGTTG+GGG 0.584787 2.4:+7902938 MS.gene31282:CDS
GAAGTCTTAATACAAAACTC+AGG 0.592108 2.4:-7903063 None:intergenic
AGAATCTCCAAAATCAACGT+TGG 0.592692 2.4:-7902338 None:intergenic
GTGATTGCGAAGGATGTTCA+CGG 0.594396 2.4:+7902652 MS.gene31282:CDS
GAGAGAGGAGAATAAGTTGG+GGG 0.597200 2.4:+7902939 MS.gene31282:CDS
TGTGGTCTTGGACCTTATGG+TGG 0.601918 2.4:+7902898 MS.gene31282:CDS
AAAGTCAGCACTAGCACTAG+TGG 0.602500 2.4:-7904670 None:intergenic
AACTCAGGAGTACTTGCTCT+AGG 0.603509 2.4:-7903048 None:intergenic
GGTATTCGTCGCGCGAAGAG+AGG 0.605126 2.4:+7902823 MS.gene31282:CDS
GCACGCATGTGCTGGAGGGA+TGG 0.614096 2.4:+7902249 MS.gene31282:CDS
TTCTTGGAGTCAGAGGATGT+TGG 0.618317 2.4:+7904732 MS.gene31282:CDS
ATGTGAAGCGAGTAAGTCCA+TGG 0.621045 2.4:+7904049 MS.gene31282:CDS
CAATCTGATGCAAACAATGG+TGG 0.622207 2.4:+7902550 MS.gene31282:CDS
GCCACTCTACAGAGGATGAG+TGG 0.626388 2.4:-7902373 None:intergenic
TGTACCTGAAGAAGTCTCCA+AGG 0.627522 2.4:-7903234 None:intergenic
GAAGCGAGTAAGTCCATGGT+TGG 0.628522 2.4:+7904053 MS.gene31282:CDS
CTTGAAAATGATGATTCTGA+TGG 0.628837 2.4:+7902475 MS.gene31282:CDS
GGTTCAGACGGTGATTGCGA+AGG 0.635341 2.4:+7902642 MS.gene31282:CDS
GTACCTGAAGAAGTCTCCAA+GGG 0.636379 2.4:-7903233 None:intergenic
AGTGAGTGGTGAATCAGTGA+AGG 0.641115 2.4:+7902975 MS.gene31282:CDS
GAAGAAGATATACAACACAA+AGG 0.641708 2.4:-7904219 None:intergenic
CCTGGCTCCCTGAATGCCTG+CGG 0.642327 2.4:-7904262 None:intergenic
AGAGAGGAGAATAAGTTGGG+GGG 0.643897 2.4:+7902940 MS.gene31282:CDS
CTGATGCAAACAATGGTGGA+GGG 0.644135 2.4:+7902554 MS.gene31282:CDS
AGTTCTGGTAATGGAAATTG+TGG 0.651495 2.4:+7902880 MS.gene31282:CDS
AATGATGATTCTGATGGTGA+TGG 0.651505 2.4:+7902481 MS.gene31282:CDS
GGTGGAAGAGTGAAAGTGAG+TGG 0.652214 2.4:+7902961 MS.gene31282:CDS
TTACCAGAACTCCATCCAGA+AGG 0.662357 2.4:-7902868 None:intergenic
GATTCTATTGTGTTTCTGAG+AGG 0.663178 2.4:+7902778 MS.gene31282:CDS
GGAACTAGGAATGCTGAACG+TGG 0.666679 2.4:+7905219 MS.gene31282:CDS
TATGGCACGCATGTGCTGGA+GGG 0.669654 2.4:+7902245 MS.gene31282:CDS
GAAACTGGACAACATCGAGA+GGG 0.678914 2.4:-7904164 None:intergenic
TCACAGAAGCCACTCTACAG+AGG 0.682462 2.4:-7902381 None:intergenic
ACTCTACAGAGGATGAGTGG+TGG 0.690773 2.4:-7902370 None:intergenic
AAGCTGCTATGAGAATTCAA+TGG 0.696903 2.4:+7903094 MS.gene31282:CDS
ACACAATCTGATGCAAACAA+TGG 0.698582 2.4:+7902547 MS.gene31282:CDS
TCAGCCGAGTAATCAAGCCG+AGG 0.714702 2.4:-7902613 None:intergenic
TCGCACCCTTCTCACCACCA+AGG 0.717374 2.4:+7904109 MS.gene31282:CDS
TCTATAGAGGAACACCAAGG+AGG 0.745721 2.4:+7902698 MS.gene31282:CDS
TACCTGAAGAAGTCTCCAAG+GGG 0.745887 2.4:-7903232 None:intergenic
ATATCTATAGAGGAACACCA+AGG 0.792807 2.4:+7902695 MS.gene31282:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATGTAAAATAAAATTCT+TGG + chr2.4:7903683-7903702 MS.gene31282:intron 10.0%
!! GAAATATATTCATATTCATA+TGG - chr2.4:7904937-7904956 None:intergenic 15.0%
!! TTAATCCATGAATATATTTA+TGG + chr2.4:7903711-7903730 MS.gene31282:intron 15.0%
!!! TTCTGTTTTTTATTTTGTTT+TGG + chr2.4:7903293-7903312 MS.gene31282:intron 15.0%
!! ACACTAACTGAAATAATATT+TGG + chr2.4:7903529-7903548 MS.gene31282:intron 20.0%
!! ATTCAATATTTACTAGAAAC+TGG - chr2.4:7903486-7903505 None:intergenic 20.0%
!! TACAACCATAAATATATTCA+TGG - chr2.4:7903719-7903738 None:intergenic 20.0%
!! TATGTATAATGTATAACCAA+TGG + chr2.4:7903442-7903461 MS.gene31282:intron 20.0%
!! TCAAATTGGAATATCTTTAT+CGG + chr2.4:7904290-7904309 MS.gene31282:CDS 20.0%
!! TTGTTGAATTATTATTTGCA+GGG + chr2.4:7905185-7905204 MS.gene31282:intron 20.0%
!! TTTGTTGAATTATTATTTGC+AGG + chr2.4:7905184-7905203 MS.gene31282:intron 20.0%
!!! ATCTAAAATACTCTTGTTTA+TGG + chr2.4:7903601-7903620 MS.gene31282:intron 20.0%
!!! TGTTTATATTTAAGACTAGA+GGG + chr2.4:7903877-7903896 MS.gene31282:intron 20.0%
!!! TTGTTTATATTTAAGACTAG+AGG + chr2.4:7903876-7903895 MS.gene31282:intron 20.0%
! AAAAAGATTGACAATTCTGA+TGG + chr2.4:7905037-7905056 MS.gene31282:CDS 25.0%
! AAAATACTTCATCAGTTTCA+GGG - chr2.4:7902420-7902439 None:intergenic 25.0%
! AAACAAGAAAAATACCTGAA+GGG - chr2.4:7904903-7904922 None:intergenic 25.0%
! AACAACAACAAATTTCAGTT+AGG + chr2.4:7904324-7904343 MS.gene31282:CDS 25.0%
! AACAACCTTGATTGAATTTA+TGG + chr2.4:7903978-7903997 MS.gene31282:intron 25.0%
! AAGAAGATATACAACACAAA+GGG - chr2.4:7904221-7904240 None:intergenic 25.0%
! AATATTTGGTATTTACCTAC+CGG + chr2.4:7903543-7903562 MS.gene31282:intron 25.0%
! GAAAAACAAATTCCAAACAT+AGG - chr2.4:7903276-7903295 None:intergenic 25.0%
! TATTGCCATAAATTCAATCA+AGG - chr2.4:7903986-7904005 None:intergenic 25.0%
! TGAAGAACTTTATAGAAAAC+TGG + chr2.4:7904788-7904807 MS.gene31282:CDS 25.0%
! TTTCTTTGCTCTTATTATCA+TGG - chr2.4:7904404-7904423 None:intergenic 25.0%
! TTTGTGTTGTATATCTTCTT+CGG + chr2.4:7904221-7904240 MS.gene31282:CDS 25.0%
!! AAAATGTTGGTATTTGAAAC+TGG - chr2.4:7904182-7904201 None:intergenic 25.0%
!! ACGTAAACTTTTTACATTGT+CGG + chr2.4:7904981-7905000 MS.gene31282:intron 25.0%
!! GTTTATATTTAAGACTAGAG+GGG + chr2.4:7903878-7903897 MS.gene31282:intron 25.0%
!! TTTCAAATACCAACATTTTC+AGG + chr2.4:7904183-7904202 MS.gene31282:CDS 25.0%
!!! AAAGACTCTTTTTTAGAGTT+TGG + chr2.4:7903399-7903418 MS.gene31282:intron 25.0%
!!! ACTGAAATTTGTTGTTGTTA+AGG - chr2.4:7904323-7904342 None:intergenic 25.0%
!!! ATTTTCAAGCTGTTTTGTTA+AGG + chr2.4:7903359-7903378 MS.gene31282:intron 25.0%
AAATTCTCACAAGAGAAGTT+TGG + chr2.4:7904449-7904468 MS.gene31282:CDS 30.0%
AAGTTTAGGCATATCTATAG+AGG + chr2.4:7902685-7902704 MS.gene31282:CDS 30.0%
AGCAAACATGCATATGTATT+TGG + chr2.4:7903823-7903842 MS.gene31282:intron 30.0%
ATACAACACAAAGGGTTAAA+AGG - chr2.4:7904213-7904232 None:intergenic 30.0%
ATTAGATACTGGTCATTGTA+AGG + chr2.4:7904707-7904726 MS.gene31282:CDS 30.0%
CTTGAAAATGATGATTCTGA+TGG + chr2.4:7902475-7902494 MS.gene31282:CDS 30.0%
GAAAATACTTCATCAGTTTC+AGG - chr2.4:7902421-7902440 None:intergenic 30.0%
GAAACAAGAAAAATACCTGA+AGG - chr2.4:7904904-7904923 None:intergenic 30.0%
GAAGAAGATATACAACACAA+AGG - chr2.4:7904222-7904241 None:intergenic 30.0%
GAAGAATCTTCAGTTTCAAA+TGG - chr2.4:7903144-7903163 None:intergenic 30.0%
GAAGTCTTAATACAAAACTC+AGG - chr2.4:7903066-7903085 None:intergenic 30.0%
GATATTCATGAATTAAGCCT+TGG - chr2.4:7903584-7903603 None:intergenic 30.0%
GTTGACCAAAAAGCAAAAAT+TGG - chr2.4:7904506-7904525 None:intergenic 30.0%
TACAACACAAAGGGTTAAAA+GGG - chr2.4:7904212-7904231 None:intergenic 30.0%
TGCTAGTAATCAAACATTTG+AGG + chr2.4:7903014-7903033 MS.gene31282:CDS 30.0%
TTAGATGCATCAAATAAGAC+AGG + chr2.4:7905243-7905262 MS.gene31282:CDS 30.0%
TTCACATTGTGTAGTAAATC+TGG - chr2.4:7904036-7904055 None:intergenic 30.0%
TTGAAACTGAAGATTCTTCT+AGG + chr2.4:7903145-7903164 MS.gene31282:CDS 30.0%
TTTGCTCTTATTATCATGGT+TGG - chr2.4:7904400-7904419 None:intergenic 30.0%
! AAGTGTTTTACTTTCCACAA+GGG + chr2.4:7902296-7902315 MS.gene31282:CDS 30.0%
! ATATCATGTCTGTTGACTAT+AGG + chr2.4:7904426-7904445 MS.gene31282:CDS 30.0%
! TCTTATTTCAAACAAGCGAA+TGG + chr2.4:7903642-7903661 MS.gene31282:intron 30.0%
!! AAAGTGTTTTACTTTCCACA+AGG + chr2.4:7902295-7902314 MS.gene31282:CDS 30.0%
!! AAGATTCTTCTAGGATTAGT+TGG + chr2.4:7903154-7903173 MS.gene31282:CDS 30.0%
!! AATACTCTTGTTCTAGAGTT+TGG + chr2.4:7903762-7903781 MS.gene31282:intron 30.0%
!! AGACACCAATTTTTGCTTTT+TGG + chr2.4:7904498-7904517 MS.gene31282:CDS 30.0%
!! CCTAATGTTTGTTGTTTACT+CGG + chr2.4:7903789-7903808 MS.gene31282:intron 30.0%
!! CTAATGTTTGTTGTTTACTC+GGG + chr2.4:7903790-7903809 MS.gene31282:intron 30.0%
!! TCAAGCTCTGAATTTCTTAA+GGG - chr2.4:7902460-7902479 None:intergenic 30.0%
!! TTCAAGCTCTGAATTTCTTA+AGG - chr2.4:7902461-7902480 None:intergenic 30.0%
!!! TTTTTCTGCTTTTTTGAGAG+AGG + chr2.4:7902924-7902943 MS.gene31282:CDS 30.0%
AAAGCTTAGATCCACAACTA+TGG + chr2.4:7902227-7902246 MS.gene31282:CDS 35.0%
AAAGTCTTTGCAAAAGATGC+AGG - chr2.4:7902526-7902545 None:intergenic 35.0%
AAGCATTTGAGCAAAACCAT+TGG - chr2.4:7903461-7903480 None:intergenic 35.0%
AAGCTGCTATGAGAATTCAA+TGG + chr2.4:7903094-7903113 MS.gene31282:CDS 35.0%
ACACAATCTGATGCAAACAA+TGG + chr2.4:7902547-7902566 MS.gene31282:CDS 35.0%
AGAAAAAGGACAAAGAGAAG+TGG + chr2.4:7904586-7904605 MS.gene31282:CDS 35.0%
AGAAAACTGGCTAAGATGTT+TGG + chr2.4:7904801-7904820 MS.gene31282:CDS 35.0%
AGAATCTCCAAAATCAACGT+TGG - chr2.4:7902341-7902360 None:intergenic 35.0%
AGGTCTTTAGATGTGTATGA+AGG + chr2.4:7905149-7905168 MS.gene31282:intron 35.0%
ATATCTATAGAGGAACACCA+AGG + chr2.4:7902695-7902714 MS.gene31282:CDS 35.0%
ATGAGAATTCAATGGTGTTC+TGG + chr2.4:7903102-7903121 MS.gene31282:CDS 35.0%
CCGAGTAAACAACAAACATT+AGG - chr2.4:7903792-7903811 None:intergenic 35.0%
GAAATCGTCTCTCGATTATT+TGG - chr2.4:7904357-7904376 None:intergenic 35.0%
GATCAAATTGAGCTCAACTA+AGG - chr2.4:7903934-7903953 None:intergenic 35.0%
GTGTCTTTAGATGTGTATGA+AGG + chr2.4:7905129-7905148 MS.gene31282:intron 35.0%
TGAGAATTCAATGGTGTTCT+GGG + chr2.4:7903103-7903122 MS.gene31282:CDS 35.0%
TTCAGTTAGTGTTCGAACTT+GGG - chr2.4:7903521-7903540 None:intergenic 35.0%
TTGAGAGAGGAGAATAAGTT+GGG + chr2.4:7902937-7902956 MS.gene31282:CDS 35.0%
TTTCAGTTAGTGTTCGAACT+TGG - chr2.4:7903522-7903541 None:intergenic 35.0%
TTTGAGAGAGGAGAATAAGT+TGG + chr2.4:7902936-7902955 MS.gene31282:CDS 35.0%
! AACACTTTTGTGTTCACTGA+TGG - chr2.4:7902283-7902302 None:intergenic 35.0%
! AATGATGATTCTGATGGTGA+TGG + chr2.4:7902481-7902500 MS.gene31282:CDS 35.0%
! ATTTGTTGCTCAGTGAGAAT+TGG - chr2.4:7904528-7904547 None:intergenic 35.0%
! CTTCTCTTTGTCCTTTTTCT+TGG - chr2.4:7904586-7904605 None:intergenic 35.0%
! GAAAGTGGAGAACTTTTTGT+TGG + chr2.4:7902802-7902821 MS.gene31282:CDS 35.0%
! GATTCTATTGTGTTTCTGAG+AGG + chr2.4:7902778-7902797 MS.gene31282:CDS 35.0%
! TGGTCATTGTAAGGTTTTCT+TGG + chr2.4:7904716-7904735 MS.gene31282:CDS 35.0%
! TGTGTTGTTGTCCAAGAAAA+AGG + chr2.4:7904572-7904591 MS.gene31282:CDS 35.0%
!! AATCAGAAAAAGCTTGTTGC+AGG + chr2.4:7902754-7902773 MS.gene31282:CDS 35.0%
!! AGTTCTGGTAATGGAAATTG+TGG + chr2.4:7902880-7902899 MS.gene31282:CDS 35.0%
!! TCTAGGATTAGTTGGTTCAT+GGG + chr2.4:7903162-7903181 MS.gene31282:CDS 35.0%
!! TTCTAGGATTAGTTGGTTCA+TGG + chr2.4:7903161-7903180 MS.gene31282:CDS 35.0%
!! TTGACAATTCTGATGGATTC+AGG + chr2.4:7905044-7905063 MS.gene31282:CDS 35.0%
!!! TGTTTTGGAGAACATGTTTG+TGG + chr2.4:7903308-7903327 MS.gene31282:intron 35.0%
AAAGGGTTGCCTGAAAATGT+TGG - chr2.4:7904195-7904214 None:intergenic 40.0%
AAGCCGAGGAAAAATCGTTT+CGG - chr2.4:7902602-7902621 None:intergenic 40.0%
AATCCGGATGTTGTGGAAAA+CGG - chr2.4:7904143-7904162 None:intergenic 40.0%
ACCAACACAAGACAAGTCTA+AGG - chr2.4:7904763-7904782 None:intergenic 40.0%
ACCTTAGACTTGTCTTGTGT+TGG + chr2.4:7904759-7904778 MS.gene31282:CDS 40.0%
ACGGTGAGATTTGGAAGTTT+AGG + chr2.4:7902671-7902690 MS.gene31282:CDS 40.0%
AGACTAGAGGGGATTTCATT+TGG + chr2.4:7903889-7903908 MS.gene31282:intron 40.0%
AGGTTGTTGATCCTATTCGT+TGG + chr2.4:7903202-7903221 MS.gene31282:CDS 40.0%
ATTTGCAGGGTTTGTATCAC+AGG + chr2.4:7905198-7905217 MS.gene31282:intron 40.0%
CAATCTGATGCAAACAATGG+TGG + chr2.4:7902550-7902569 MS.gene31282:CDS 40.0%
CTACTCTCTTGTCCTATGTT+TGG + chr2.4:7903261-7903280 MS.gene31282:intron 40.0%
GTTGTTAACAAATGCCTCCT+TGG - chr2.4:7902715-7902734 None:intergenic 40.0%
TATGGCAATAACACAACTGC+AGG + chr2.4:7903996-7904015 MS.gene31282:intron 40.0%
TATTCCAATTTGAGCATGCC+TGG - chr2.4:7904283-7904302 None:intergenic 40.0%
TGAGAGAGGAGAATAAGTTG+GGG + chr2.4:7902938-7902957 MS.gene31282:CDS 40.0%
! AATTGTGGTCTTGGACCTTA+TGG + chr2.4:7902895-7902914 MS.gene31282:CDS 40.0%
! CTAGTGGTAGAAGATTTTGC+TGG - chr2.4:7904657-7904676 None:intergenic 40.0%
! GAGAGGGATTGTTAATGGAT+TGG + chr2.4:7902840-7902859 MS.gene31282:CDS 40.0%
! GGCATTTGTTAACAACTGGT+TGG + chr2.4:7902719-7902738 MS.gene31282:CDS 40.0%
! GGGAACTATTTCTTCTGTTC+AGG + chr2.4:7903182-7903201 MS.gene31282:CDS 40.0%
! TAAGGTTTTCTTGGAGTCAG+AGG + chr2.4:7904725-7904744 MS.gene31282:CDS 40.0%
! TGGCAACTTGGATTAGATAC+TGG + chr2.4:7904696-7904715 MS.gene31282:CDS 40.0%
!! AATGGATTGGAAACACCTTC+TGG + chr2.4:7902853-7902872 MS.gene31282:CDS 40.0%
!! ACAGGTGCTGTTAAACAAAC+TGG + chr2.4:7904870-7904889 MS.gene31282:CDS 40.0%
!! AGGAGGCATTTGTTAACAAC+TGG + chr2.4:7902715-7902734 MS.gene31282:CDS 40.0%
!! GGTAATGGAAATTGTGGTCT+TGG + chr2.4:7902886-7902905 MS.gene31282:CDS 40.0%
!! TTTTCCTCGGCTTGATTACT+CGG + chr2.4:7902609-7902628 MS.gene31282:CDS 40.0%
!!! GCACAATCCAACGTTGATTT+TGG + chr2.4:7902331-7902350 MS.gene31282:CDS 40.0%
AAACGGAGTTTCTTCCTTGG+TGG - chr2.4:7904126-7904145 None:intergenic 45.0%
AAACTGGTGAAGAACCCTTC+AGG + chr2.4:7904886-7904905 MS.gene31282:CDS 45.0%
AAAGTCAGCACTAGCACTAG+TGG - chr2.4:7904673-7904692 None:intergenic 45.0%
AACACAACTGCAGGTCACTT+GGG + chr2.4:7904005-7904024 MS.gene31282:intron 45.0%
ACAACATCGAGAGGGAAATC+CGG - chr2.4:7904159-7904178 None:intergenic 45.0%
AGAAGTCTCCAAGGGGAATT+AGG - chr2.4:7903228-7903247 None:intergenic 45.0%
AGGATGTTCACGGTGAGATT+TGG + chr2.4:7902662-7902681 MS.gene31282:CDS 45.0%
AGTGAGTGGTGAATCAGTGA+AGG + chr2.4:7902975-7902994 MS.gene31282:CDS 45.0%
ATCGTTTCGGCACAGTATCT+AGG - chr2.4:7902589-7902608 None:intergenic 45.0%
ATGTGAAGCGAGTAAGTCCA+TGG + chr2.4:7904049-7904068 MS.gene31282:CDS 45.0%
CAGGCAGCAAAGATACTAGA+AGG + chr2.4:7905063-7905082 MS.gene31282:CDS 45.0%
GAAACTGGACAACATCGAGA+GGG - chr2.4:7904167-7904186 None:intergenic 45.0%
GATACCAATTCGACCAACCA+TGG - chr2.4:7904069-7904088 None:intergenic 45.0%
GATTGGAAACACCTTCTGGA+TGG + chr2.4:7902857-7902876 MS.gene31282:CDS 45.0%
GCGAAGAGAGGGATTGTTAA+TGG + chr2.4:7902835-7902854 MS.gene31282:CDS 45.0%
GGAAAACGGAGTTTCTTCCT+TGG - chr2.4:7904129-7904148 None:intergenic 45.0%
GGGTTTGTATCACAGGAACT+AGG + chr2.4:7905205-7905224 MS.gene31282:intron 45.0%
GTACCTGAAGAAGTCTCCAA+GGG - chr2.4:7903236-7903255 None:intergenic 45.0%
GTGATTGCGAAGGATGTTCA+CGG + chr2.4:7902652-7902671 MS.gene31282:CDS 45.0%
TAACACAACTGCAGGTCACT+TGG + chr2.4:7904004-7904023 MS.gene31282:intron 45.0%
TACCTGAAGAAGTCTCCAAG+GGG - chr2.4:7903235-7903254 None:intergenic 45.0%
TCTATAGAGGAACACCAAGG+AGG + chr2.4:7902698-7902717 MS.gene31282:CDS 45.0%
TCTGATGCAAACAATGGTGG+AGG + chr2.4:7902553-7902572 MS.gene31282:CDS 45.0%
TGAAACTGGACAACATCGAG+AGG - chr2.4:7904168-7904187 None:intergenic 45.0%
TGTACCTGAAGAAGTCTCCA+AGG - chr2.4:7903237-7903256 None:intergenic 45.0%
TTACCAGAACTCCATCCAGA+AGG - chr2.4:7902871-7902890 None:intergenic 45.0%
TTCTTGGAGTCAGAGGATGT+TGG + chr2.4:7904732-7904751 MS.gene31282:CDS 45.0%
TTGGCTATCAAGAGCTTGAC+CGG - chr2.4:7903565-7903584 None:intergenic 45.0%
! AAGTCCATGGTTGGTCGAAT+TGG + chr2.4:7904062-7904081 MS.gene31282:CDS 45.0%
! ACTCCGTTTTCCACAACATC+CGG + chr2.4:7904137-7904156 MS.gene31282:CDS 45.0%
! AGAGAGGAGAATAAGTTGGG+GGG + chr2.4:7902940-7902959 MS.gene31282:CDS 45.0%
! CTGATGCAAACAATGGTGGA+GGG + chr2.4:7902554-7902573 MS.gene31282:CDS 45.0%
! GAGAGAGGAGAATAAGTTGG+GGG + chr2.4:7902939-7902958 MS.gene31282:CDS 45.0%
! GTGCCGAAACGATTTTTCCT+CGG + chr2.4:7902596-7902615 MS.gene31282:CDS 45.0%
! GTGTTTCTGAGAGGTGAAAG+TGG + chr2.4:7902787-7902806 MS.gene31282:CDS 45.0%
! TCTGGATGGAGTTCTGGTAA+TGG + chr2.4:7902871-7902890 MS.gene31282:CDS 45.0%
!! AACTCAGGAGTACTTGCTCT+AGG - chr2.4:7903051-7903070 None:intergenic 45.0%
!! GCAGAAAAAGCACCACCATA+AGG - chr2.4:7902913-7902932 None:intergenic 45.0%
!! GCTGACTTTTGTTGGCAACT+TGG + chr2.4:7904684-7904703 MS.gene31282:CDS 45.0%
!! TTCAATGGTGTTCTGGGATG+AGG + chr2.4:7903109-7903128 MS.gene31282:CDS 45.0%
!! TTCTTCCTTGGTGGTGAGAA+GGG - chr2.4:7904117-7904136 None:intergenic 45.0%
!! TTTCTTCCTTGGTGGTGAGA+AGG - chr2.4:7904118-7904137 None:intergenic 45.0%
!!! GTGCTAGTGCTGACTTTTGT+TGG + chr2.4:7904676-7904695 MS.gene31282:CDS 45.0%
AATTATAACGCTCCTGCCGC+AGG + chr2.4:7904246-7904265 MS.gene31282:CDS 50.0%
ACACCTTCTGGATGGAGTTC+TGG + chr2.4:7902865-7902884 MS.gene31282:CDS 50.0%
ACCACTCATCCTCTGTAGAG+TGG + chr2.4:7902372-7902391 MS.gene31282:CDS 50.0%
ACTCTACAGAGGATGAGTGG+TGG - chr2.4:7902373-7902392 None:intergenic 50.0%
AGAGGGAAATCCGGATGTTG+TGG - chr2.4:7904150-7904169 None:intergenic 50.0%
CTATCAAGAGCTTGACCGGT+AGG - chr2.4:7903561-7903580 None:intergenic 50.0%
GAAGCGAGTAAGTCCATGGT+TGG + chr2.4:7904053-7904072 MS.gene31282:CDS 50.0%
GGAACTAGGAATGCTGAACG+TGG + chr2.4:7905219-7905238 MS.gene31282:CDS 50.0%
GGGGAATTAGGCCAACGAAT+AGG - chr2.4:7903216-7903235 None:intergenic 50.0%
GGTGGAAGAGTGAAAGTGAG+TGG + chr2.4:7902961-7902980 MS.gene31282:CDS 50.0%
GTACTCTATCGCGATGCAAC+AGG + chr2.4:7904852-7904871 MS.gene31282:CDS 50.0%
TCACAGAAGCCACTCTACAG+AGG - chr2.4:7902384-7902403 None:intergenic 50.0%
TTCCCCTTGGAGACTTCTTC+AGG + chr2.4:7903230-7903249 MS.gene31282:CDS 50.0%
TTCGCAATCACCGTCTGAAC+CGG - chr2.4:7902643-7902662 None:intergenic 50.0%
! GGTCAAGCTCTTGATAGCCA+AGG + chr2.4:7903564-7903583 MS.gene31282:intron 50.0%
! TGTGGTCTTGGACCTTATGG+TGG + chr2.4:7902898-7902917 MS.gene31282:CDS 50.0%
! TTCGTTGGCCTAATTCCCCT+TGG + chr2.4:7903217-7903236 MS.gene31282:CDS 50.0%
CAACTATGGCACGCATGTGC+TGG + chr2.4:7902241-7902260 MS.gene31282:CDS 55.0%
GCACATGCGTGCCATAGTTG+TGG - chr2.4:7902241-7902260 None:intergenic 55.0%
GCATGTTCTGCATGCCCTTG+TGG - chr2.4:7902313-7902332 None:intergenic 55.0%
GCCACTCTACAGAGGATGAG+TGG - chr2.4:7902376-7902395 None:intergenic 55.0%
GGAGCCAGGCATGCTCAAAT+TGG + chr2.4:7904276-7904295 MS.gene31282:CDS 55.0%
GGTTCAGACGGTGATTGCGA+AGG + chr2.4:7902642-7902661 MS.gene31282:CDS 55.0%
GTATTCGTCGCGCGAAGAGA+GGG + chr2.4:7902824-7902843 MS.gene31282:CDS 55.0%
TATGGCACGCATGTGCTGGA+GGG + chr2.4:7902245-7902264 MS.gene31282:CDS 55.0%
TCAGCCGAGTAATCAAGCCG+AGG - chr2.4:7902616-7902635 None:intergenic 55.0%
TGATTACTCGGCTGAGCCAC+CGG + chr2.4:7902621-7902640 MS.gene31282:CDS 55.0%
! GAGGAGAATAAGTTGGGGGG+TGG + chr2.4:7902943-7902962 MS.gene31282:CDS 55.0%
CTATGGCACGCATGTGCTGG+AGG + chr2.4:7902244-7902263 MS.gene31282:CDS 60.0%
GCAATCACCGTCTGAACCGG+TGG - chr2.4:7902640-7902659 None:intergenic 60.0%
GGTATTCGTCGCGCGAAGAG+AGG + chr2.4:7902823-7902842 MS.gene31282:CDS 60.0%
TCGCACCCTTCTCACCACCA+AGG + chr2.4:7904109-7904128 MS.gene31282:CDS 60.0%
CCTGGCTCCCTGAATGCCTG+CGG - chr2.4:7904265-7904284 None:intergenic 65.0%
GCACGCATGTGCTGGAGGGA+TGG + chr2.4:7902249-7902268 MS.gene31282:CDS 65.0%
GCTCCTGCCGCAGGCATTCA+GGG + chr2.4:7904255-7904274 MS.gene31282:CDS 65.0%
!! GGCTGAGCCACCGGTTCAGA+CGG + chr2.4:7902630-7902649 MS.gene31282:CDS 65.0%
CCGCAGGCATTCAGGGAGCC+AGG + chr2.4:7904262-7904281 MS.gene31282:CDS 70.0%
CGCTCCTGCCGCAGGCATTC+AGG + chr2.4:7904254-7904273 MS.gene31282:CDS 70.0%
GCTCCCTGAATGCCTGCGGC+AGG - chr2.4:7904261-7904280 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 7902211 7905287 7902211 ID=MS.gene31282
chr2.4 mRNA 7902211 7905287 7902211 ID=MS.gene31282.t1;Parent=MS.gene31282
chr2.4 exon 7902211 7903251 7902211 ID=MS.gene31282.t1.exon1;Parent=MS.gene31282.t1
chr2.4 CDS 7902211 7903251 7902211 ID=cds.MS.gene31282.t1;Parent=MS.gene31282.t1
chr2.4 exon 7904018 7904907 7904018 ID=MS.gene31282.t1.exon2;Parent=MS.gene31282.t1
chr2.4 CDS 7904018 7904907 7904018 ID=cds.MS.gene31282.t1;Parent=MS.gene31282.t1
chr2.4 exon 7905019 7905084 7905019 ID=MS.gene31282.t1.exon3;Parent=MS.gene31282.t1
chr2.4 CDS 7905019 7905084 7905019 ID=cds.MS.gene31282.t1;Parent=MS.gene31282.t1
chr2.4 exon 7905206 7905287 7905206 ID=MS.gene31282.t1.exon4;Parent=MS.gene31282.t1
chr2.4 CDS 7905206 7905287 7905206 ID=cds.MS.gene31282.t1;Parent=MS.gene31282.t1
Gene Sequence

>MS.gene31282

ATGAAAGAAGCTGAGAAAAGCTTAGATCCACAACTATGGCACGCATGTGCTGGAGGGATGGTTCAAATGCCATCAGTGAACACAAAAGTGTTTTACTTTCCACAAGGGCATGCAGAACATGCACAATCCAACGTTGATTTTGGAGATTCTTTCAGAATTCCACCACTCATCCTCTGTAGAGTGGCTTCTGTGAAATTCTTAGCTGACCCTGAAACTGATGAAGTATTTTCAAAGATAACTTTGATTCCCTTAAGAAATTCAGAGCTTGAAAATGATGATTCTGATGGTGATGGTTCAGAGAATTCAGAAAAGCCTGCATCTTTTGCAAAGACTTTGACACAATCTGATGCAAACAATGGTGGAGGGTTCTCTGTTCCTAGATACTGTGCCGAAACGATTTTTCCTCGGCTTGATTACTCGGCTGAGCCACCGGTTCAGACGGTGATTGCGAAGGATGTTCACGGTGAGATTTGGAAGTTTAGGCATATCTATAGAGGAACACCAAGGAGGCATTTGTTAACAACTGGTTGGAGTAGTTTTGTGAATCAGAAAAAGCTTGTTGCAGGTGATTCTATTGTGTTTCTGAGAGGTGAAAGTGGAGAACTTTTTGTTGGTATTCGTCGCGCGAAGAGAGGGATTGTTAATGGATTGGAAACACCTTCTGGATGGAGTTCTGGTAATGGAAATTGTGGTCTTGGACCTTATGGTGGTGCTTTTTCTGCTTTTTTGAGAGAGGAGAATAAGTTGGGGGGTGGAAGAGTGAAAGTGAGTGGTGAATCAGTGAAGGAAGCTATGAGACTTGCTGCTAGTAATCAAACATTTGAGGTTGTTTATTATCCTAGAGCAAGTACTCCTGAGTTTTGTATTAAGACTTCAGCTGTGAAAGCTGCTATGAGAATTCAATGGTGTTCTGGGATGAGGTTTAAGATGCCATTTGAAACTGAAGATTCTTCTAGGATTAGTTGGTTCATGGGAACTATTTCTTCTGTTCAGGTTGTTGATCCTATTCGTTGGCCTAATTCCCCTTGGAGACTTCTTCAGGTACAACTACTACTCTCTTGTCCTATGTTTGGAATTTGTTTTTCTGTTTTTTATTTTGTTTTGGAGAACATGTTTGTGGTAATAAGTTTCATTTGAATTTTGATGATATTTTCAAGCTGTTTTGTTAAGGTTCATTTCTTTGAATTGAAAGACTCTTTTTTAGAGTTTGGTTATGTTAATATGTGTATAATATGTATAATGTATAACCAATGGTTTTGCTCAAATGCTTAACCAGTTTCTAGTAAATATTGAATTGATCGAGAGTACCCAAGTTCGAACACTAACTGAAATAATATTTGGTATTTACCTACCGGTCAAGCTCTTGATAGCCAAGGCTTAATTCATGAATATCTAAAATACTCTTGTTTATGGTTGTACTTATCGCTTGTTTCTTATTTCAAACAAGCGAATGGTTAAAACAAAACAAAAAAATTATGTAAAATAAAATTCTTGGAGCTCTTAATCCATGAATATATTTATGGTTGTACTTATTGCTTGTTTCTATGTAAAAATACTCTTGTTCTAGAGTTTGGAATACCTAATGTTTGTTGTTTACTCGGGTTTGTATTTAAGCAAACATGCATATGTATTTGGTCTAAATATAGATCAAATATATTTATATTATTGTTTATATTTAAGACTAGAGGGGATTTCATTTGGTGATTTGATTGTATTCAAACCTTAGTTGAGCTCAATTTGATCATAAAGAAATGCTTGTCATGCAACAACAACCTTGATTGAATTTATGGCAATAACACAACTGCAGGTCACTTGGGACGAACCAGATTTACTACACAATGTGAAGCGAGTAAGTCCATGGTTGGTCGAATTGGTATCAAACATGTCGATTATTCATCTCGCACCCTTCTCACCACCAAGGAAGAAACTCCGTTTTCCACAACATCCGGATTTCCCTCTCGATGTTGTCCAGTTTCAAATACCAACATTTTCAGGCAACCCTTTTAACCCTTTGTGTTGTATATCTTCTTCGGATAATTATAACGCTCCTGCCGCAGGCATTCAGGGAGCCAGGCATGCTCAAATTGGAATATCTTTATCGGATCTTCACCTTAACAACAACAAATTTCAGTTAGGTGTGTTTCCAAATAATCGAGAGACGATTTCTAATGTTAGCAACATAACTACCAACCATGATAATAAGAGCAAAGAAAGTATATCATGTCTGTTGACTATAGGAAATTCTCACAAGAGAAGTTTGGAGATTAAATCTGATAATAATGATAAGAGACACCAATTTTTGCTTTTTGGTCAACCAATTCTCACTGAGCAACAAATCTCTAGAAAATTTTCTAGTGATGATGTGTTGTTGTCCAAGAAAAAGGACAAAGAGAAGTGGTTTTTCAGTGATACTACTCAATCTTCTATTTCTGAACAGTTTTCACCAGCAAAATCTTCTACCACTAGTGCTAGTGCTGACTTTTGTTGGCAACTTGGATTAGATACTGGTCATTGTAAGGTTTTCTTGGAGTCAGAGGATGTTGGAAGAACCTTAGACTTGTCTTGTGTTGGTTCTTATGAAGAACTTTATAGAAAACTGGCTAAGATGTTTGGAATAGAAAGATCTGAGATGTTGAGTCGTGTACTCTATCGCGATGCAACAGGTGCTGTTAAACAAACTGGTGAAGAACCCTTCAGGTATTTTTCTTGTTTCATCTTTTTATCCATATGAATATGAATATATTTCGAGTTTAATTTATATGCATCGTCGACGTAAACTTTTTACATTGTCGGTTAATTACATTGTAGTGACTTCATGAAGACAGCAAAAAGATTGACAATTCTGATGGATTCAGGCAGCAAAGATACTAGAAGGTGTGATTAAAAAAAAAAACATTGTTATATTTAATTATTAGTTAGTGTCTTTAGATGTGTATGAAGGTCTTTAGATGTGTATGAAGGTACTAAACATTTTTTGTTGAATTATTATTTGCAGGGTTTGTATCACAGGAACTAGGAATGCTGAACGTGGATTAGATGCATCAAATAAGACAGGACCTATGAGCATCTTTGTTTAA
Protein sequence

>MS.gene31282.t1

MKEAEKSLDPQLWHACAGGMVQMPSVNTKVFYFPQGHAEHAQSNVDFGDSFRIPPLILCRVASVKFLADPETDEVFSKITLIPLRNSELENDDSDGDGSENSEKPASFAKTLTQSDANNGGGFSVPRYCAETIFPRLDYSAEPPVQTVIAKDVHGEIWKFRHIYRGTPRRHLLTTGWSSFVNQKKLVAGDSIVFLRGESGELFVGIRRAKRGIVNGLETPSGWSSGNGNCGLGPYGGAFSAFLREENKLGGGRVKVSGESVKEAMRLAASNQTFEVVYYPRASTPEFCIKTSAVKAAMRIQWCSGMRFKMPFETEDSSRISWFMGTISSVQVVDPIRWPNSPWRLLQVTWDEPDLLHNVKRVSPWLVELVSNMSIIHLAPFSPPRKKLRFPQHPDFPLDVVQFQIPTFSGNPFNPLCCISSSDNYNAPAAGIQGARHAQIGISLSDLHLNNNKFQLGVFPNNRETISNVSNITTNHDNKSKESISCLLTIGNSHKRSLEIKSDNNDKRHQFLLFGQPILTEQQISRKFSSDDVLLSKKKDKEKWFFSDTTQSSISEQFSPAKSSTTSASADFCWQLGLDTGHCKVFLESEDVGRTLDLSCVGSYEELYRKLAKMFGIERSEMLSRVLYRDATGAVKQTGEEPFSDFMKTAKRLTILMDSGSKDTRRVCITGTRNAERGLDASNKTGPMSIFV