Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049919.t1 | GAU47155.1 | 90.2 | 112 | 11 | 0 | 1 | 112 | 121 | 232 | 1.90E-52 | 214.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049919.t1 | Q9M2A1 | 69.6 | 112 | 33 | 1 | 1 | 112 | 305 | 415 | 3.1e-40 | 165.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049919.t1 | A0A2Z6PDC7 | 90.2 | 112 | 11 | 0 | 1 | 112 | 121 | 232 | 1.3e-52 | 214.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049919 | MS.gene054379 | 0.805934 | 1.05E-49 | -1.69E-46 |
| MS.gene049919 | MS.gene059725 | 0.813259 | 2.80E-51 | -1.69E-46 |
| MS.gene049919 | MS.gene45193 | 0.804159 | 2.47E-49 | -1.69E-46 |
| MS.gene049919 | MS.gene49486 | 0.809171 | 2.16E-50 | -1.69E-46 |
| MS.gene049919 | MS.gene57160 | -0.803212 | 3.88E-49 | -1.69E-46 |
| MS.gene049919 | MS.gene57162 | -0.807951 | 3.93E-50 | -1.69E-46 |
| MS.gene049919 | MS.gene68874 | 0.801009 | 1.10E-48 | -1.69E-46 |
| MS.gene049919 | MS.gene81998 | 0.800881 | 1.17E-48 | -1.69E-46 |
| MS.gene049919 | MS.gene84388 | 0.803668 | 3.12E-49 | -1.69E-46 |
| MS.gene049919 | MS.gene87264 | 0.808718 | 2.70E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049919.t1 | MTR_1g051205 | 88.393 | 112 | 13 | 0 | 1 | 112 | 287 | 398 | 8.47e-71 | 216 |
| MS.gene049919.t1 | MTR_0004s0610 | 89.286 | 112 | 12 | 0 | 1 | 112 | 288 | 399 | 1.19e-70 | 216 |
| MS.gene049919.t1 | MTR_5g068170 | 87.500 | 96 | 12 | 0 | 16 | 111 | 215 | 310 | 4.23e-55 | 174 |
| MS.gene049919.t1 | MTR_6g034745 | 74.468 | 47 | 12 | 0 | 9 | 55 | 271 | 317 | 7.47e-18 | 77.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049919.t1 | AT3G42630 | 69.643 | 112 | 33 | 1 | 1 | 112 | 305 | 415 | 7.95e-52 | 168 |
Find 25 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCGAGAATGGCGTTGTTT+TGG | 0.214618 | 5.3:+53274424 | None:intergenic |
| TAGAAGGCTTGGAAGGAATT+TGG | 0.229388 | 5.3:+53274536 | MS.gene049919:CDS |
| ATCGGAGGGACCAAATATTT+TGG | 0.238103 | 5.3:+53274739 | MS.gene049919:CDS |
| GTTAGTAGTTCCAAAATATT+TGG | 0.264609 | 5.3:-53274749 | None:intergenic |
| TGAAAAGATGGTTCCTGATT+TGG | 0.287044 | 5.3:+53274479 | MS.gene049919:CDS |
| AACAGCATGAGTGGAGTTAT+TGG | 0.292095 | 5.3:+53274685 | MS.gene049919:CDS |
| ACAGCATGAGTGGAGTTATT+GGG | 0.381574 | 5.3:+53274686 | MS.gene049919:CDS |
| TCGATGCTTACTTGGATAGA+AGG | 0.404396 | 5.3:+53274520 | MS.gene049919:CDS |
| CCCTCCGATACTGTTTCTTA+AGG | 0.406605 | 5.3:-53274725 | None:intergenic |
| GACTGATCCGTTTGTGTTTG+AGG | 0.412453 | 5.3:+53274605 | MS.gene049919:CDS |
| CAACCACAAGTAACCAAATC+AGG | 0.433446 | 5.3:-53274492 | None:intergenic |
| AGGTGACTTTCACTTGAGTT+CGG | 0.460453 | 5.3:+53274638 | MS.gene049919:CDS |
| TTGTGTTGTCGATGCTTACT+TGG | 0.486590 | 5.3:+53274512 | MS.gene049919:CDS |
| GCTTACTTGGATAGAAGGCT+TGG | 0.489558 | 5.3:+53274525 | MS.gene049919:CDS |
| GTTCCTGATTTGGTTACTTG+TGG | 0.517330 | 5.3:+53274489 | MS.gene049919:CDS |
| CCTAAAACCTCAAACACAAA+CGG | 0.528045 | 5.3:-53274612 | None:intergenic |
| ACTTGGATAGAAGGCTTGGA+AGG | 0.547884 | 5.3:+53274529 | MS.gene049919:CDS |
| ACATATGAGAAGTGAAAAGA+TGG | 0.561414 | 5.3:+53274467 | MS.gene049919:CDS |
| ACAAGACACAACAGCATGAG+TGG | 0.583417 | 5.3:+53274676 | MS.gene049919:CDS |
| ACCTTAAGAAACAGTATCGG+AGG | 0.594441 | 5.3:+53274724 | MS.gene049919:CDS |
| AATACCTTAAGAAACAGTAT+CGG | 0.622014 | 5.3:+53274721 | MS.gene049919:CDS |
| TGACTTTCACTTGAGTTCGG+AGG | 0.628971 | 5.3:+53274641 | MS.gene049919:CDS |
| CCTTAAGAAACAGTATCGGA+GGG | 0.648546 | 5.3:+53274725 | MS.gene049919:CDS |
| AACGGATCAGTCAACAACCG+CGG | 0.728087 | 5.3:-53274594 | None:intergenic |
| TGGATGTGGATGATTGTCCG+CGG | 0.790662 | 5.3:+53274577 | MS.gene049919:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATACCTTAAGAAACAGTAT+CGG | + | chr5.3:53274721-53274740 | MS.gene049919:CDS | 25.0% |
| !!! | GGAGTTTGTTTTGAATAAAA+TGG | + | chr5.3:53274557-53274576 | MS.gene049919:CDS | 25.0% |
| !!! | TGTTTTGAATAAAATGGATG+TGG | + | chr5.3:53274563-53274582 | MS.gene049919:CDS | 25.0% |
| ACATATGAGAAGTGAAAAGA+TGG | + | chr5.3:53274467-53274486 | MS.gene049919:CDS | 30.0% | |
| CCTAAAACCTCAAACACAAA+CGG | - | chr5.3:53274615-53274634 | None:intergenic | 35.0% | |
| TGAAAAGATGGTTCCTGATT+TGG | + | chr5.3:53274479-53274498 | MS.gene049919:CDS | 35.0% | |
| !!! | GTGTTTGAGGTTTTAGGTAA+AGG | + | chr5.3:53274618-53274637 | MS.gene049919:CDS | 35.0% |
| AACAGCATGAGTGGAGTTAT+TGG | + | chr5.3:53274685-53274704 | MS.gene049919:CDS | 40.0% | |
| ACAGCATGAGTGGAGTTATT+GGG | + | chr5.3:53274686-53274705 | MS.gene049919:CDS | 40.0% | |
| AGGTGACTTTCACTTGAGTT+CGG | + | chr5.3:53274638-53274657 | MS.gene049919:CDS | 40.0% | |
| CAACCACAAGTAACCAAATC+AGG | - | chr5.3:53274495-53274514 | None:intergenic | 40.0% | |
| TAGAAGGCTTGGAAGGAATT+TGG | + | chr5.3:53274536-53274555 | MS.gene049919:CDS | 40.0% | |
| TCGATGCTTACTTGGATAGA+AGG | + | chr5.3:53274520-53274539 | MS.gene049919:CDS | 40.0% | |
| TTGTGTTGTCGATGCTTACT+TGG | + | chr5.3:53274512-53274531 | MS.gene049919:CDS | 40.0% | |
| ! | ACCTTAAGAAACAGTATCGG+AGG | + | chr5.3:53274724-53274743 | MS.gene049919:CDS | 40.0% |
| ! | ATCGGAGGGACCAAATATTT+TGG | + | chr5.3:53274739-53274758 | MS.gene049919:CDS | 40.0% |
| ! | CCTTAAGAAACAGTATCGGA+GGG | + | chr5.3:53274725-53274744 | MS.gene049919:CDS | 40.0% |
| !! | CCGTTTGTGTTTGAGGTTTT+AGG | + | chr5.3:53274612-53274631 | MS.gene049919:CDS | 40.0% |
| !! | GTTCCTGATTTGGTTACTTG+TGG | + | chr5.3:53274489-53274508 | MS.gene049919:CDS | 40.0% |
| ACAAGACACAACAGCATGAG+TGG | + | chr5.3:53274676-53274695 | MS.gene049919:CDS | 45.0% | |
| ACTTGGATAGAAGGCTTGGA+AGG | + | chr5.3:53274529-53274548 | MS.gene049919:CDS | 45.0% | |
| GACTGATCCGTTTGTGTTTG+AGG | + | chr5.3:53274605-53274624 | MS.gene049919:CDS | 45.0% | |
| GCTTACTTGGATAGAAGGCT+TGG | + | chr5.3:53274525-53274544 | MS.gene049919:CDS | 45.0% | |
| TGACTTTCACTTGAGTTCGG+AGG | + | chr5.3:53274641-53274660 | MS.gene049919:CDS | 45.0% | |
| ! | CCCTCCGATACTGTTTCTTA+AGG | - | chr5.3:53274728-53274747 | None:intergenic | 45.0% |
| AACGGATCAGTCAACAACCG+CGG | - | chr5.3:53274597-53274616 | None:intergenic | 50.0% | |
| TGGATGTGGATGATTGTCCG+CGG | + | chr5.3:53274577-53274596 | MS.gene049919:CDS | 50.0% | |
| !!! | CTTGAGTTCGGAGGCGTTTT+TGG | + | chr5.3:53274650-53274669 | MS.gene049919:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 53274432 | 53274770 | 53274432 | ID=MS.gene049919 |
| chr5.3 | mRNA | 53274432 | 53274770 | 53274432 | ID=MS.gene049919.t1;Parent=MS.gene049919 |
| chr5.3 | exon | 53274432 | 53274770 | 53274432 | ID=MS.gene049919.t1.exon1;Parent=MS.gene049919.t1 |
| chr5.3 | CDS | 53274432 | 53274770 | 53274432 | ID=cds.MS.gene049919.t1;Parent=MS.gene049919.t1 |
| Gene Sequence |
| Protein sequence |
