AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68874


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68874.t1 MTR_4g132780 98.400 250 4 0 1 250 240 489 4.47e-175 503
MS.gene68874.t1 MTR_2g025130 46.957 230 77 4 21 250 51 235 5.56e-52 176
MS.gene68874.t1 MTR_1g012840 35.648 216 81 4 37 250 262 421 1.19e-34 132
MS.gene68874.t1 MTR_2g451540 38.117 223 87 6 28 250 272 443 9.60e-34 130
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68874.t1 AT1G62390 59.533 257 82 3 3 253 254 494 1.61e-77 250
MS.gene68874.t1 AT4G32070 37.383 214 89 5 37 250 318 486 2.03e-37 140
MS.gene68874.t1 AT4G32070 37.383 214 89 5 37 250 318 486 2.03e-37 140
MS.gene68874.t1 AT2G25290 38.249 217 83 5 37 250 281 449 8.01e-37 139
MS.gene68874.t1 AT2G25290 38.249 217 83 5 37 250 281 449 8.01e-37 139
MS.gene68874.t1 AT2G25290 38.249 217 83 5 37 250 281 449 8.01e-37 139
MS.gene68874.t1 AT2G25290 38.249 217 83 5 37 250 281 449 8.01e-37 139
MS.gene68874.t1 AT5G20360 37.383 214 79 5 37 250 270 428 3.54e-31 122
MS.gene68874.t1 AT5G20360 37.383 214 79 5 37 250 270 428 3.54e-31 122
MS.gene68874.t1 AT5G20360 37.383 214 79 5 37 250 300 458 5.74e-31 122
MS.gene68874.t1 AT5G20360 37.383 214 79 5 37 250 300 458 5.74e-31 122
MS.gene68874.t1 AT5G20360 37.383 214 79 5 37 250 350 508 8.26e-31 122
MS.gene68874.t1 AT5G20360 37.383 214 79 5 37 250 360 518 9.50e-31 121
MS.gene68874.t1 AT5G20360 37.383 214 79 5 37 250 360 518 9.50e-31 121

Find 67 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGCATTGGCATTCTTCAAC+TGG 0.265168 8.4:+1539066 MS.gene68874:CDS
ACAGCAGGACCATTATTAAA+AGG 0.297119 8.4:-1538289 None:intergenic
AAAGGCTTCAGGACAACTTT+AGG 0.315931 8.4:-1538271 None:intergenic
ATCTTATCAACCTCGGTATC+AGG 0.324081 8.4:-1538790 None:intergenic
GACCATTATTAAAAGGCTTC+AGG 0.343074 8.4:-1538282 None:intergenic
TAAGGCTGCTTCAAAGTTCC+AGG 0.355515 8.4:+1539037 MS.gene68874:CDS
AGATAAAAGTGATTCTATTT+CGG 0.361509 8.4:+1538603 MS.gene68874:CDS
CCGACAGATTCCTATCCTTC+TGG 0.372691 8.4:-1538336 None:intergenic
CAAGGATTGTGATGGTGATT+TGG 0.383636 8.4:+1538504 MS.gene68874:CDS
TGCAAGTCAATATGAGCATC+AGG 0.394649 8.4:-1538940 None:intergenic
AAAGAAAAGCCTGGAGCAAC+GGG 0.403258 8.4:+1538835 MS.gene68874:CDS
TCAGTGACTTCTGTAACTTC+AGG 0.407687 8.4:-1538763 None:intergenic
ATGCCAATGCCGCCACCTCC+TGG 0.407999 8.4:-1539055 None:intergenic
GGAGCAGGAGCCACCTTTGT+TGG 0.416587 8.4:+1538657 MS.gene68874:CDS
CATATTGACTTGCATGAGCT+TGG 0.418574 8.4:+1538949 MS.gene68874:CDS
TCATTATCTCCCGTTGCTCC+AGG 0.430465 8.4:-1538844 None:intergenic
GTTCCAGGAGGTGGCGGCAT+TGG 0.433016 8.4:+1539052 MS.gene68874:CDS
AACAAAGGTGGCTCCTGCTC+CGG 0.440439 8.4:-1538655 None:intergenic
TTGTTTATGATCATGACATC+AGG 0.448132 8.4:+1538389 MS.gene68874:CDS
TTCGAGTTAGATTGCACAGC+AGG 0.450987 8.4:-1538304 None:intergenic
ATCTGTCGGAGGTTTCTATT+CGG 0.466887 8.4:+1538350 MS.gene68874:CDS
TGATCATAAACAAGCTTCAA+TGG 0.469664 8.4:-1538379 None:intergenic
AGATCCACTTTCATCTCCCT+TGG 0.469667 8.4:-1538716 None:intergenic
ATATTGACTTGCATGAGCTT+GGG 0.472334 8.4:+1538950 MS.gene68874:CDS
GCCACCTTTGTTGGAAGAAG+AGG 0.478587 8.4:+1538666 MS.gene68874:CDS
CAGTGACAAGTGAGAAGCTC+AGG 0.478949 8.4:+1539004 MS.gene68874:CDS
TGCTTCAAAGTTCCAGGAGG+TGG 0.481900 8.4:+1539043 MS.gene68874:CDS
AGCTCAGGATCTATTTGATA+AGG 0.490348 8.4:+1539019 MS.gene68874:CDS
CAGTGACTTCTGTAACTTCA+GGG 0.493370 8.4:-1538762 None:intergenic
TCTTCCTCTTCTTCCAACAA+AGG 0.496918 8.4:-1538670 None:intergenic
TCCTCTTCTTCCAACAAAGG+TGG 0.505242 8.4:-1538667 None:intergenic
GGCATTGGCATTCTTCAACT+GGG 0.505652 8.4:+1539067 MS.gene68874:CDS
TTCAGTTCTGATCAAGTACA+AGG 0.507255 8.4:+1538486 MS.gene68874:CDS
TGTAGAAGTTAGTCCGGAGC+AGG 0.507291 8.4:+1538642 MS.gene68874:CDS
ATCTACTGATGAACTCAGAT+TGG 0.510115 8.4:+1538537 MS.gene68874:CDS
AAAATGAGAGCCAGAAGGAT+AGG 0.530714 8.4:+1538326 MS.gene68874:CDS
GATGTTTCAAAAGAAAAGCC+TGG 0.534064 8.4:+1538826 MS.gene68874:CDS
AGTGGATCTCATTCTTCCCT+CGG 0.536060 8.4:+1538730 MS.gene68874:CDS
CATCAGGCTTGCTCAGATGC+CGG 0.537347 8.4:+1538405 MS.gene68874:CDS
ACATATTGTAGAAGTTAGTC+CGG 0.538956 8.4:+1538636 MS.gene68874:CDS
TGATAAGATAATAACAAAGA+AGG 0.539178 8.4:+1538804 MS.gene68874:CDS
AAAAGAAAAGCCTGGAGCAA+CGG 0.543981 8.4:+1538834 MS.gene68874:CDS
ATGTTCACATGTGCGCTGCT+AGG 0.546089 8.4:+1539093 MS.gene68874:CDS
AAGAAGTGGAGATGGATGAT+TGG 0.546355 8.4:+1538872 MS.gene68874:CDS
TTCAAAGTTCCAGGAGGTGG+CGG 0.547550 8.4:+1539046 MS.gene68874:CDS
TGTTATTATCTTATCAACCT+CGG 0.548455 8.4:-1538797 None:intergenic
GCATTGGCATTCTTCAACTG+GGG 0.567327 8.4:+1539068 MS.gene68874:CDS
TGAATGCAAAGAAGTGGAGA+TGG 0.567536 8.4:+1538864 MS.gene68874:CDS
GTGACCAAGGGAGATGAAAG+TGG 0.568869 8.4:+1538712 MS.gene68874:CDS
TGTCGGAGGTTTCTATTCGG+TGG 0.583849 8.4:+1538353 MS.gene68874:CDS
CACTGAAGTTCCTGATACCG+AGG 0.590229 8.4:+1538780 MS.gene68874:CDS
ACTCTGAAACTGCAGTTCAC+CGG 0.593018 8.4:-1538424 None:intergenic
TTTCAGAGTACTTAGAGATG+TGG 0.594565 8.4:+1538438 MS.gene68874:CDS
AGATAATGAATGCAAAGAAG+TGG 0.599861 8.4:+1538858 MS.gene68874:CDS
ATCAGAACTGAATTTGACGA+AGG 0.601722 8.4:-1538475 None:intergenic
TGACTTGCATGAGCTTGGGA+TGG 0.605109 8.4:+1538954 MS.gene68874:CDS
CCAGAAGGATAGGAATCTGT+CGG 0.613760 8.4:+1538336 MS.gene68874:CDS
CGTTGAGAATGAAGTGACCA+AGG 0.628866 8.4:+1538699 MS.gene68874:CDS
TGGGATGGAGCTATGTTCAG+AGG 0.646712 8.4:+1538969 MS.gene68874:CDS
GCAAGTCAATATGAGCATCA+GGG 0.659898 8.4:-1538939 None:intergenic
GTTGAGAATGAAGTGACCAA+GGG 0.663815 8.4:+1538700 MS.gene68874:CDS
TTCAGGGACAGAGTCACCGA+GGG 0.668184 8.4:-1538746 None:intergenic
GGTCAATACCAACATGTGAG+CGG 0.673952 8.4:-1538918 None:intergenic
ATCAAGTACAAGGATTGTGA+TGG 0.683892 8.4:+1538496 MS.gene68874:CDS
GAAGGATAGGAATCTGTCGG+AGG 0.709195 8.4:+1538339 MS.gene68874:CDS
CTTCAGGGACAGAGTCACCG+AGG 0.713125 8.4:-1538747 None:intergenic
GGCTGCTTCAAAGTTCCAGG+AGG 0.723223 8.4:+1539040 MS.gene68874:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGATAAAAGTGATTCTATTT+CGG + chr8.4:1538603-1538622 MS.gene68874:CDS 20.0%
!! TGATAAGATAATAACAAAGA+AGG + chr8.4:1538804-1538823 MS.gene68874:CDS 20.0%
! TGTTATTATCTTATCAACCT+CGG - chr8.4:1538800-1538819 None:intergenic 25.0%
ACATATTGTAGAAGTTAGTC+CGG + chr8.4:1538636-1538655 MS.gene68874:CDS 30.0%
AGATAATGAATGCAAAGAAG+TGG + chr8.4:1538858-1538877 MS.gene68874:CDS 30.0%
TGATCATAAACAAGCTTCAA+TGG - chr8.4:1538382-1538401 None:intergenic 30.0%
TTGTTTATGATCATGACATC+AGG + chr8.4:1538389-1538408 MS.gene68874:CDS 30.0%
ACAGCAGGACCATTATTAAA+AGG - chr8.4:1538292-1538311 None:intergenic 35.0%
AGCTCAGGATCTATTTGATA+AGG + chr8.4:1539019-1539038 MS.gene68874:CDS 35.0%
ATCAAGTACAAGGATTGTGA+TGG + chr8.4:1538496-1538515 MS.gene68874:CDS 35.0%
ATCAGAACTGAATTTGACGA+AGG - chr8.4:1538478-1538497 None:intergenic 35.0%
ATCTACTGATGAACTCAGAT+TGG + chr8.4:1538537-1538556 MS.gene68874:CDS 35.0%
GACCATTATTAAAAGGCTTC+AGG - chr8.4:1538285-1538304 None:intergenic 35.0%
GATGTTTCAAAAGAAAAGCC+TGG + chr8.4:1538826-1538845 MS.gene68874:CDS 35.0%
! GTCCTGAAGCCTTTTAATAA+TGG + chr8.4:1538280-1538299 MS.gene68874:CDS 35.0%
! TTCAGTTCTGATCAAGTACA+AGG + chr8.4:1538486-1538505 MS.gene68874:CDS 35.0%
! TTTCAGAGTACTTAGAGATG+TGG + chr8.4:1538438-1538457 MS.gene68874:CDS 35.0%
!! ATATTGACTTGCATGAGCTT+GGG + chr8.4:1538950-1538969 MS.gene68874:CDS 35.0%
AAAAGAAAAGCCTGGAGCAA+CGG + chr8.4:1538834-1538853 MS.gene68874:CDS 40.0%
AAAATGAGAGCCAGAAGGAT+AGG + chr8.4:1538326-1538345 MS.gene68874:CDS 40.0%
AAAGGCTTCAGGACAACTTT+AGG - chr8.4:1538274-1538293 None:intergenic 40.0%
AAGAAGTGGAGATGGATGAT+TGG + chr8.4:1538872-1538891 MS.gene68874:CDS 40.0%
ATCTGTCGGAGGTTTCTATT+CGG + chr8.4:1538350-1538369 MS.gene68874:CDS 40.0%
ATCTTATCAACCTCGGTATC+AGG - chr8.4:1538793-1538812 None:intergenic 40.0%
CAGTGACTTCTGTAACTTCA+GGG - chr8.4:1538765-1538784 None:intergenic 40.0%
GCAAGTCAATATGAGCATCA+GGG - chr8.4:1538942-1538961 None:intergenic 40.0%
GTTGAGAATGAAGTGACCAA+GGG + chr8.4:1538700-1538719 MS.gene68874:CDS 40.0%
TCAGTGACTTCTGTAACTTC+AGG - chr8.4:1538766-1538785 None:intergenic 40.0%
TCTTCCTCTTCTTCCAACAA+AGG - chr8.4:1538673-1538692 None:intergenic 40.0%
TGAATGCAAAGAAGTGGAGA+TGG + chr8.4:1538864-1538883 MS.gene68874:CDS 40.0%
TGCAAGTCAATATGAGCATC+AGG - chr8.4:1538943-1538962 None:intergenic 40.0%
! CAAGGATTGTGATGGTGATT+TGG + chr8.4:1538504-1538523 MS.gene68874:CDS 40.0%
!! CATATTGACTTGCATGAGCT+TGG + chr8.4:1538949-1538968 MS.gene68874:CDS 40.0%
AAAGAAAAGCCTGGAGCAAC+GGG + chr8.4:1538835-1538854 MS.gene68874:CDS 45.0%
AGATCCACTTTCATCTCCCT+TGG - chr8.4:1538719-1538738 None:intergenic 45.0%
AGTGGATCTCATTCTTCCCT+CGG + chr8.4:1538730-1538749 MS.gene68874:CDS 45.0%
CCAGAAGGATAGGAATCTGT+CGG + chr8.4:1538336-1538355 MS.gene68874:CDS 45.0%
CGTTGAGAATGAAGTGACCA+AGG + chr8.4:1538699-1538718 MS.gene68874:CDS 45.0%
CTCGAAAAATGAGAGCCAGA+AGG + chr8.4:1538321-1538340 MS.gene68874:CDS 45.0%
GGTCAATACCAACATGTGAG+CGG - chr8.4:1538921-1538940 None:intergenic 45.0%
TAAGGCTGCTTCAAAGTTCC+AGG + chr8.4:1539037-1539056 MS.gene68874:CDS 45.0%
TCCTCTTCTTCCAACAAAGG+TGG - chr8.4:1538670-1538689 None:intergenic 45.0%
! GCATTGGCATTCTTCAACTG+GGG + chr8.4:1539068-1539087 MS.gene68874:CDS 45.0%
! TTCGAGTTAGATTGCACAGC+AGG - chr8.4:1538307-1538326 None:intergenic 45.0%
!! ACTCTGAAACTGCAGTTCAC+CGG - chr8.4:1538427-1538446 None:intergenic 45.0%
!! GGCATTGGCATTCTTCAACT+GGG + chr8.4:1539067-1539086 MS.gene68874:CDS 45.0%
ATGTTCACATGTGCGCTGCT+AGG + chr8.4:1539093-1539112 MS.gene68874:CDS 50.0%
CACTGAAGTTCCTGATACCG+AGG + chr8.4:1538780-1538799 MS.gene68874:CDS 50.0%
CAGTGACAAGTGAGAAGCTC+AGG + chr8.4:1539004-1539023 MS.gene68874:CDS 50.0%
CCGACAGATTCCTATCCTTC+TGG - chr8.4:1538339-1538358 None:intergenic 50.0%
GAAGGATAGGAATCTGTCGG+AGG + chr8.4:1538339-1538358 MS.gene68874:CDS 50.0%
GCCACCTTTGTTGGAAGAAG+AGG + chr8.4:1538666-1538685 MS.gene68874:CDS 50.0%
GTGACCAAGGGAGATGAAAG+TGG + chr8.4:1538712-1538731 MS.gene68874:CDS 50.0%
TCATTATCTCCCGTTGCTCC+AGG - chr8.4:1538847-1538866 None:intergenic 50.0%
TGCTTCAAAGTTCCAGGAGG+TGG + chr8.4:1539043-1539062 MS.gene68874:CDS 50.0%
TGGGATGGAGCTATGTTCAG+AGG + chr8.4:1538969-1538988 MS.gene68874:CDS 50.0%
TGTAGAAGTTAGTCCGGAGC+AGG + chr8.4:1538642-1538661 MS.gene68874:CDS 50.0%
TGTCGGAGGTTTCTATTCGG+TGG + chr8.4:1538353-1538372 MS.gene68874:CDS 50.0%
TTCAAAGTTCCAGGAGGTGG+CGG + chr8.4:1539046-1539065 MS.gene68874:CDS 50.0%
! CAGCTTTTCCGCTCACATGT+TGG + chr8.4:1538910-1538929 MS.gene68874:CDS 50.0%
! TGACTTGCATGAGCTTGGGA+TGG + chr8.4:1538954-1538973 MS.gene68874:CDS 50.0%
!! CGGCATTGGCATTCTTCAAC+TGG + chr8.4:1539066-1539085 MS.gene68874:CDS 50.0%
AACAAAGGTGGCTCCTGCTC+CGG - chr8.4:1538658-1538677 None:intergenic 55.0%
GGCTGCTTCAAAGTTCCAGG+AGG + chr8.4:1539040-1539059 MS.gene68874:CDS 55.0%
TTCAGGGACAGAGTCACCGA+GGG - chr8.4:1538749-1538768 None:intergenic 55.0%
! CATCAGGCTTGCTCAGATGC+CGG + chr8.4:1538405-1538424 MS.gene68874:CDS 55.0%
CTTCAGGGACAGAGTCACCG+AGG - chr8.4:1538750-1538769 None:intergenic 60.0%
GGAGCAGGAGCCACCTTTGT+TGG + chr8.4:1538657-1538676 MS.gene68874:CDS 60.0%
ATGCCAATGCCGCCACCTCC+TGG - chr8.4:1539058-1539077 None:intergenic 65.0%
! GTTCCAGGAGGTGGCGGCAT+TGG + chr8.4:1539052-1539071 MS.gene68874:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 1538268 1539131 1538268 ID=MS.gene68874
chr8.4 mRNA 1538268 1539131 1538268 ID=MS.gene68874.t1;Parent=MS.gene68874
chr8.4 exon 1538268 1539131 1538268 ID=MS.gene68874.t1.exon1;Parent=MS.gene68874.t1
chr8.4 CDS 1538268 1539131 1538268 ID=cds.MS.gene68874.t1;Parent=MS.gene68874.t1
Gene Sequence

>MS.gene68874

ATGCCTAAAGTTGTCCTGAAGCCTTTTAATAATGGTCCTGCTGTGCAATCTAACTCGAAAAATGAGAGCCAGAAGGATAGGAATCTGTCGGAGGTTTCTATTCGGTGGAGACCATTGAAGCTTGTTTATGATCATGACATCAGGCTTGCTCAGATGCCGGTGAACTGCAGTTTCAGAGTACTTAGAGATGTGGTAAGCAAAAGATTTCCTTCGTCAAATTCAGTTCTGATCAAGTACAAGGATTGTGATGGTGATTTGGTTACTATAACATCTACTGATGAACTCAGATTGGCAGAGTCGTTTGTCGATAGCAATCTGCTGAAAGAACTTGAATCAGATAAAAGTGATTCTATTTCGGTGCTGAGACTACATATTGTAGAAGTTAGTCCGGAGCAGGAGCCACCTTTGTTGGAAGAAGAGGAAGAAAAACTCGTTGAGAATGAAGTGACCAAGGGAGATGAAAGTGGATCTCATTCTTCCCTCGGTGACTCTGTCCCTGAAGTTACAGAAGTCACTGAAGTTCCTGATACCGAGGTTGATAAGATAATAACAAAGAAGGATGTTTCAAAAGAAAAGCCTGGAGCAACGGGAGATAATGAATGCAAAGAAGTGGAGATGGATGATTGGCTCTTTGAATTTGCTCAGCTTTTCCGCTCACATGTTGGTATTGACCCTGATGCTCATATTGACTTGCATGAGCTTGGGATGGAGCTATGTTCAGAGGCACTTGAAGAAACAGTGACAAGTGAGAAGCTCAGGATCTATTTGATAAGGCTGCTTCAAAGTTCCAGGAGGTGGCGGCATTGGCATTCTTCAACTGGGGTAATGTTCACATGTGCGCTGCTAGGAAGCGAATTCCCTTAG

Protein sequence

>MS.gene68874.t1

MPKVVLKPFNNGPAVQSNSKNESQKDRNLSEVSIRWRPLKLVYDHDIRLAQMPVNCSFRVLRDVVSKRFPSSNSVLIKYKDCDGDLVTITSTDELRLAESFVDSNLLKELESDKSDSISVLRLHIVEVSPEQEPPLLEEEEEKLVENEVTKGDESGSHSSLGDSVPEVTEVTEVPDTEVDKIITKKDVSKEKPGATGDNECKEVEMDDWLFEFAQLFRSHVGIDPDAHIDLHELGMELCSEALEETVTSEKLRIYLIRLLQSSRRWRHWHSSTGVMFTCALLGSEFP