AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene57162


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57162.t1 MTR_5g016560 93.443 122 8 0 17 138 87 208 4.45e-79 232
MS.gene57162.t1 MTR_5g016560 92.308 104 8 0 17 120 87 190 9.48e-65 196
MS.gene57162.t1 MTR_4g094930 78.151 119 25 1 17 135 72 189 3.56e-62 189
MS.gene57162.t1 MTR_4g070400 68.868 106 33 0 17 122 76 181 1.97e-48 154
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57162.t1 AT4G39220 65.517 116 40 0 17 132 75 190 1.94e-54 169
MS.gene57162.t1 AT4G39220 65.517 116 40 0 17 132 75 190 1.94e-54 169
MS.gene57162.t1 AT2G21600 62.295 122 45 1 17 138 75 195 5.16e-52 163
MS.gene57162.t1 AT2G23310 67.925 106 33 1 27 132 107 211 7.82e-46 148
MS.gene57162.t1 AT2G23310 67.925 106 32 2 27 132 107 210 8.43e-44 143
MS.gene57162.t1 AT2G18240 62.136 103 38 1 17 119 79 180 1.28e-37 127
MS.gene57162.t1 AT2G18240 62.136 103 38 1 17 119 79 180 1.31e-37 127

Find 35 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCCTATACTCCTCTTCTAC+TGG 0.286487 5.2:+8270128 MS.gene57162:CDS
CGTTTGATATTCCAGTCTTC+TGG 0.311008 5.2:+8270107 MS.gene57162:CDS
AGATATGTACCGTTCAATTT+CGG 0.357992 5.2:+8270205 MS.gene57162:CDS
ACCGTTCAATTTCGGAAAAC+AGG 0.388365 5.2:+8270213 MS.gene57162:CDS
CTCCGCGTCTACTTCTTAGA+AGG 0.426734 5.2:+8268662 MS.gene57162:CDS
AACTCATCGGAAGCTCTAAT+AGG 0.427663 5.2:-8268797 None:intergenic
GCCTCCCTGAGTTCAAGTTC+TGG 0.451201 5.2:+8268837 MS.gene57162:CDS
TCAGCGGCGTCGGCGACGGC+GGG 0.451738 5.2:-8268761 None:intergenic
AGGAGTATAGGCCAGAAGAC+TGG 0.477362 5.2:-8270118 None:intergenic
GTCAGCGGCGTCGGCGACGG+CGG 0.477787 5.2:-8268762 None:intergenic
ACCGTCAGCGGCGTCGGCGA+CGG 0.481145 5.2:-8268765 None:intergenic
GCCGTCGCCGACGCCGCTGA+CGG 0.482016 5.2:+8268764 MS.gene57162:CDS
GCCTATACTCCTCTTCTACT+GGG 0.482573 5.2:+8270129 MS.gene57162:CDS
AACTGAAAATTTCAGTCCAC+AGG 0.507776 5.2:-8270411 None:intergenic
ACCCAGTAGAAGAGGAGTAT+AGG 0.512859 5.2:-8270130 None:intergenic
AGTAGGACCGTCAGCGGCGT+CGG 0.515054 5.2:-8268771 None:intergenic
TATTACCAGAACTTGAACTC+AGG 0.516687 5.2:-8268842 None:intergenic
TCAGGGAGGCGGCGAACGAA+AGG 0.517684 5.2:-8268824 None:intergenic
ACAAGACCACCCAGTAGAAG+AGG 0.526419 5.2:-8270138 None:intergenic
TACGTGCTACCGCGATGGAT+AGG 0.536752 5.2:+8268611 None:intergenic
TCTTGTTCTCGCTTACTATG+AGG 0.536784 5.2:+8270155 MS.gene57162:CDS
ACGTGCTACCGCGATGGATA+GGG 0.540998 5.2:+8268612 None:intergenic
GGAGGCGGCGAACGAAAGGA+CGG 0.592422 5.2:-8268820 None:intergenic
TGTTCTCGCTTACTATGAGG+AGG 0.593117 5.2:+8270158 MS.gene57162:CDS
ATTACCAGAACTTGAACTCA+GGG 0.599316 5.2:-8268841 None:intergenic
GAAGCTCTAATAGGAAGAGT+AGG 0.606900 5.2:-8268788 None:intergenic
AGGAAGAGTAGGACCGTCAG+CGG 0.634957 5.2:-8268777 None:intergenic
AGAAAGCACAAGTCTTCCTG+TGG 0.635231 5.2:+8270395 MS.gene57162:CDS
AGAACTTGAACTCAGGGAGG+CGG 0.636334 5.2:-8268835 None:intergenic
AACGAAAGGACGGAACTCAT+CGG 0.637257 5.2:-8268810 None:intergenic
TATACTCCTCTTCTACTGGG+TGG 0.667420 5.2:+8270132 MS.gene57162:CDS
AGCCTTCTAAGAAGTAGACG+CGG 0.688638 5.2:-8268664 None:intergenic
CTTCTAAGAAGTAGACGCGG+AGG 0.701782 5.2:-8268661 None:intergenic
ACCAGAACTTGAACTCAGGG+AGG 0.720457 5.2:-8268838 None:intergenic
GGGCAGGTACTCGATCACAG+TGG 0.793101 5.2:+8270048 MS.gene57162:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGTTTTTCATAAATTATAT+TGG - chr5.2:8269735-8269754 None:intergenic 10.0%
!! AAATTATATTGGATAGCTTA+TGG - chr5.2:8269724-8269743 None:intergenic 20.0%
!! TGCATAATTATTGTTAAATG+TGG + chr5.2:8269049-8269068 MS.gene57162:intron 20.0%
!!! ATCGTTTAATTGATTGATTT+TGG + chr5.2:8268885-8268904 MS.gene57162:intron 20.0%
!!! TAATTGATTGATTTTGGTAT+AGG + chr5.2:8268891-8268910 MS.gene57162:intron 20.0%
! AATTATGCACAATATCATCA+AGG - chr5.2:8269038-8269057 None:intergenic 25.0%
! ATTAAGTTCTTTATCCAAAG+AGG + chr5.2:8269854-8269873 MS.gene57162:intron 25.0%
! TATGGAAACAACTTATGATA+GGG + chr5.2:8269674-8269693 MS.gene57162:intron 25.0%
! TTACATATGTTGACATACAA+TGG - chr5.2:8269347-8269366 None:intergenic 25.0%
! TTATGGAAACAACTTATGAT+AGG + chr5.2:8269673-8269692 MS.gene57162:intron 25.0%
! TTTGTTAGATAGTTGTTAGT+TGG + chr5.2:8269298-8269317 MS.gene57162:intron 25.0%
!! CTTCTCATCAAAATTTCTTT+TGG - chr5.2:8269160-8269179 None:intergenic 25.0%
!!! AGTTTCTTTTTCACTTAGTA+AGG + chr5.2:8269980-8269999 MS.gene57162:intron 25.0%
!!! TTGTGTTGATTGTTGTTATA+AGG + chr5.2:8270290-8270309 MS.gene57162:intron 25.0%
AGATATGTACCGTTCAATTT+CGG + chr5.2:8270205-8270224 MS.gene57162:CDS 30.0%
CCTAAAACAACATATCAATG+TGG - chr5.2:8269897-8269916 None:intergenic 30.0%
GTATATGATGAGAGTTTACT+GGG + chr5.2:8269827-8269846 MS.gene57162:intron 30.0%
TTAGATGATGTTGTCTTCTT+CGG + chr5.2:8269934-8269953 MS.gene57162:intron 30.0%
! TTCTCTTCATTTTCTTCACA+GGG + chr5.2:8269439-8269458 MS.gene57162:intron 30.0%
!!! CCACATTGATATGTTGTTTT+AGG + chr5.2:8269894-8269913 MS.gene57162:intron 30.0%
ACGAAGTGTGTATAAACTGA+CGG - chr5.2:8268981-8269000 None:intergenic 35.0%
ATGAAGAGAACCATAGAATG+TGG - chr5.2:8269429-8269448 None:intergenic 35.0%
ATTACCAGAACTTGAACTCA+GGG - chr5.2:8268844-8268863 None:intergenic 35.0%
CGTATATGATGAGAGTTTAC+TGG + chr5.2:8269826-8269845 MS.gene57162:intron 35.0%
GAAGACAACATCATCTAAGT+AGG - chr5.2:8269932-8269951 None:intergenic 35.0%
TACATCCTCAATCTTCTCAT+CGG + chr5.2:8268716-8268735 MS.gene57162:intron 35.0%
TATTACCAGAACTTGAACTC+AGG - chr5.2:8268845-8268864 None:intergenic 35.0%
TCAAATAAACCAACCCAAAC+AGG - chr5.2:8269465-8269484 None:intergenic 35.0%
TGACGGTAATGAATGATTGA+CGG - chr5.2:8268964-8268983 None:intergenic 35.0%
! ACCTGTTTTCCGAAATTGAA+CGG - chr5.2:8270217-8270236 None:intergenic 35.0%
! AGCTGCTCAATTTTCTTTTG+TGG + chr5.2:8270027-8270046 MS.gene57162:intron 35.0%
! CAATCTAGCTTGCAATATCA+AGG - chr5.2:8269093-8269112 None:intergenic 35.0%
! GCTGCTCAATTTTCTTTTGT+GGG + chr5.2:8270028-8270047 MS.gene57162:intron 35.0%
! GTTCTCTTCATTTTCTTCAC+AGG + chr5.2:8269438-8269457 MS.gene57162:intron 35.0%
! TTGTTGTTGTGATTGTTGTG+TGG + chr5.2:8270252-8270271 MS.gene57162:intron 35.0%
AATGTGGAAGACCCAAACTA+TGG - chr5.2:8269881-8269900 None:intergenic 40.0%
ACCGTTCAATTTCGGAAAAC+AGG + chr5.2:8270213-8270232 MS.gene57162:CDS 40.0%
ACTTCGTTCTCGTGTGATTA+AGG + chr5.2:8268994-8269013 MS.gene57162:intron 40.0%
AGAACCATAGAATGTGGTTG+AGG - chr5.2:8269423-8269442 None:intergenic 40.0%
AGACTGTTTCAGAGAGCTTA+TGG + chr5.2:8269656-8269675 MS.gene57162:intron 40.0%
ATCCAAAGAGGACCATAGTT+TGG + chr5.2:8269866-8269885 MS.gene57162:intron 40.0%
CGTTTGATATTCCAGTCTTC+TGG + chr5.2:8270107-8270126 MS.gene57162:CDS 40.0%
TCCAAAGAGGACCATAGTTT+GGG + chr5.2:8269867-8269886 MS.gene57162:intron 40.0%
TCTTGTTCTCGCTTACTATG+AGG + chr5.2:8270155-8270174 MS.gene57162:CDS 40.0%
! AACTCATCGGAAGCTCTAAT+AGG - chr5.2:8268800-8268819 None:intergenic 40.0%
! CTCAATTTTCTTTTGTGGGC+AGG + chr5.2:8270032-8270051 MS.gene57162:intron 40.0%
! GAAGCTCTAATAGGAAGAGT+AGG - chr5.2:8268791-8268810 None:intergenic 40.0%
! GATGTTGTCTTCTTCGGTTT+TGG + chr5.2:8269940-8269959 MS.gene57162:intron 40.0%
AACGAAAGGACGGAACTCAT+CGG - chr5.2:8268813-8268832 None:intergenic 45.0%
ACCCAAACTATGGTCCTCTT+TGG - chr5.2:8269871-8269890 None:intergenic 45.0%
CACTTAGTAAGGTCTGCAGA+AGG + chr5.2:8269991-8270010 MS.gene57162:intron 45.0%
GAGTCCTCAACCACATTCTA+TGG + chr5.2:8269416-8269435 MS.gene57162:intron 45.0%
GCCTATACTCCTCTTCTACT+GGG + chr5.2:8270129-8270148 MS.gene57162:CDS 45.0%
GGAAACCGATGAGAAGATTG+AGG - chr5.2:8268724-8268743 None:intergenic 45.0%
TATACTCCTCTTCTACTGGG+TGG + chr5.2:8270132-8270151 MS.gene57162:CDS 45.0%
TGCAAGCTAGATTGTGCATG+CGG + chr5.2:8269099-8269118 MS.gene57162:intron 45.0%
TGTTCTCGCTTACTATGAGG+AGG + chr5.2:8270158-8270177 MS.gene57162:CDS 45.0%
TTTCTTCACAGGGCCTGTTT+GGG + chr5.2:8269449-8269468 MS.gene57162:intron 45.0%
! ACCCAGTAGAAGAGGAGTAT+AGG - chr5.2:8270133-8270152 None:intergenic 45.0%
! AGAAAGCACAAGTCTTCCTG+TGG + chr5.2:8270395-8270414 MS.gene57162:CDS 45.0%
! AGCCTTCTAAGAAGTAGACG+CGG - chr5.2:8268667-8268686 None:intergenic 45.0%
! TTTTCTTCACAGGGCCTGTT+TGG + chr5.2:8269448-8269467 MS.gene57162:intron 45.0%
ACAAGACCACCCAGTAGAAG+AGG - chr5.2:8270141-8270160 None:intergenic 50.0%
ACCAGAACTTGAACTCAGGG+AGG - chr5.2:8268841-8268860 None:intergenic 50.0%
AGAACTTGAACTCAGGGAGG+CGG - chr5.2:8268838-8268857 None:intergenic 50.0%
CTCCGCGTCTACTTCTTAGA+AGG + chr5.2:8268662-8268681 MS.gene57162:CDS 50.0%
GGCCTATACTCCTCTTCTAC+TGG + chr5.2:8270128-8270147 MS.gene57162:CDS 50.0%
GGCTTCTACGTCGTTACGTA+CGG + chr5.2:8268683-8268702 MS.gene57162:intron 50.0%
GTGGTTGAGGACTCAGAAAC+TGG - chr5.2:8269410-8269429 None:intergenic 50.0%
TACGTCGTTACGTACGGTCT+TGG + chr5.2:8268689-8268708 MS.gene57162:intron 50.0%
! AGGAGTATAGGCCAGAAGAC+TGG - chr5.2:8270121-8270140 None:intergenic 50.0%
! CTTCTAAGAAGTAGACGCGG+AGG - chr5.2:8268664-8268683 None:intergenic 50.0%
AGGAAGAGTAGGACCGTCAG+CGG - chr5.2:8268780-8268799 None:intergenic 55.0%
TTCACAGGGCCTGTTTGGGT+TGG + chr5.2:8269453-8269472 MS.gene57162:intron 55.0%
! GCCTCCCTGAGTTCAAGTTC+TGG + chr5.2:8268837-8268856 MS.gene57162:CDS 55.0%
GGGCAGGTACTCGATCACAG+TGG + chr5.2:8270048-8270067 MS.gene57162:intron 60.0%
!! CATCGGTTTCCTCTCCCCTC+AGG + chr5.2:8268733-8268752 MS.gene57162:intron 60.0%
AGTAGGACCGTCAGCGGCGT+CGG - chr5.2:8268774-8268793 None:intergenic 65.0%
CGACGGCGGGATCAACCTGA+GGG - chr5.2:8268751-8268770 None:intergenic 65.0%
CGGGATCAACCTGAGGGGAG+AGG - chr5.2:8268745-8268764 None:intergenic 65.0%
GACGGCGGGATCAACCTGAG+GGG - chr5.2:8268750-8268769 None:intergenic 65.0%
GGAGGCGGCGAACGAAAGGA+CGG - chr5.2:8268823-8268842 None:intergenic 65.0%
TCAGGGAGGCGGCGAACGAA+AGG - chr5.2:8268827-8268846 None:intergenic 65.0%
GCGACGGCGGGATCAACCTG+AGG - chr5.2:8268752-8268771 None:intergenic 70.0%
!! ACCGTCAGCGGCGTCGGCGA+CGG - chr5.2:8268768-8268787 None:intergenic 75.0%
! GCCGTCGCCGACGCCGCTGA+CGG + chr5.2:8268764-8268783 MS.gene57162:CDS 80.0%
!!! GTCAGCGGCGTCGGCGACGG+CGG - chr5.2:8268765-8268784 None:intergenic 80.0%
!!! TCAGCGGCGTCGGCGACGGC+GGG - chr5.2:8268764-8268783 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 8268625 8270422 8268625 ID=MS.gene57162
chr5.2 mRNA 8268625 8270422 8268625 ID=MS.gene57162.t1;Parent=MS.gene57162
chr5.2 exon 8268625 8268699 8268625 ID=MS.gene57162.t1.exon1;Parent=MS.gene57162.t1
chr5.2 CDS 8268625 8268699 8268625 ID=cds.MS.gene57162.t1;Parent=MS.gene57162.t1
chr5.2 exon 8268755 8268858 8268755 ID=MS.gene57162.t1.exon2;Parent=MS.gene57162.t1
chr5.2 CDS 8268755 8268858 8268755 ID=cds.MS.gene57162.t1;Parent=MS.gene57162.t1
chr5.2 exon 8270054 8270234 8270054 ID=MS.gene57162.t1.exon3;Parent=MS.gene57162.t1
chr5.2 CDS 8270054 8270234 8270054 ID=cds.MS.gene57162.t1;Parent=MS.gene57162.t1
chr5.2 exon 8270366 8270422 8270366 ID=MS.gene57162.t1.exon4;Parent=MS.gene57162.t1
chr5.2 CDS 8270366 8270422 8270366 ID=cds.MS.gene57162.t1;Parent=MS.gene57162.t1
Gene Sequence

>MS.gene57162

ATGGATAGGGTTTTCGGTCGTCGCTTTCATCTACATCCTCCGCGTCTACTTCTTAGAAGGCTTCTACGTCGTTACGTACGGTCTTGGCATTTACATCCTCAATCTTCTCATCGGTTTCCTCTCCCCTCAGGTTGATCCCGCCGTCGCCGACGCCGCTGACGGTCCTACTCTTCCTATTAGAGCTTCCGATGAGTTCCGTCCTTTCGTTCGCCGCCTCCCTGAGTTCAAGTTCTGGTAATACTTCAATTTGATTATCAATTATCGTTTAATTGATTGATTTTGGTATAGGATTTAATTCATATACACTGTCAGTGTAAAGAGTTATGAGTTTTTACACCGTCAATCATTCATTACCGTCAGTTTATACACACTTCGTTCTCGTGTGATTAAGGTTATAGTTGCGTTATGATCCTTGATGATATTGTGCATAATTATTGTTAAATGTGGTTGATAATTGATGTTGTACCTTGATATTGCAAGCTAGATTGTGCATGCGGACATTCATTTAAAACGTTCTATGTGATGATGAGTTCCAAAAGAAATTTTGATGAGAAGAATGTTTTGCTGTTAGTTTCTTATTATGATCTTTGTCGATGTATCGAAAACAATAATCATGTAATGTACTTCATTGCATGAAGCTATTGTTGAATCACAGAGTTAAGTTAATTATAATTTTGTTAGATAGTTGTTAGTTGGTTATGCTAGCAATCTGCTAACTACCATTGTATGTCAACATATGTAAGCTTATTGTTCTTGTATTCTTTTAACATTATTGAGTTGAACCAGTTTCTGAGTCCTCAACCACATTCTATGGTTCTCTTCATTTTCTTCACAGGGCCTGTTTGGGTTGGTTTATTTGAGTTTATCTATTAGCATCAACACATAAGCTTATTGTTGTCGTATTCTTTTAACGTTATTGAGTTGAATCAGTTTCTGAGTCCTCAACCACATTCTATGGTTCTCTTCATTTTCTTCACAGGGCCTGTTTGGGTTGGTTTATTTGAGTTTATCTATTAGCATTAACACACATGAGACTGTTTCAGAGAGCTTATGGAAACAACTTATGATAGGGCTATAATTTGTTTTCAGCTTATTTCCATAAGCTATCCAATATAATTTATGAAAAACAACTTATAGCTTATATGTAAACATTTTGATATTATCTTTTGTTAATGAAAGATTAACAACTTATACATAAGCACGTATATGATGAGAGTTTACTGGGTTTAATTAAGTTCTTTATCCAAAGAGGACCATAGTTTGGGTCTTCCACATTGATATGTTGTTTTAGGCATAAAAAAGTGCCTACTTAGATGATGTTGTCTTCTTCGGTTTTGGTGCTCTTAAGTTGTTGAAGTTTCTTTTTCACTTAGTAAGGTCTGCAGAAGGTTGCTTTTACTAAAGCTGCTCAATTTTCTTTTGTGGGCAGGTACTCGATCACAGTGGCATTTTGCATTGCAATTGTGATGACTTTCTTTAGTGCGTTTGATATTCCAGTCTTCTGGCCTATACTCCTCTTCTACTGGGTGGTCTTGTTCTCGCTTACTATGAGGAGGCAGATATCACATATGATCAAATACAGATATGTACCGTTCAATTTCGGAAAACAGGTAATTTAGATTCAAAATTGTTGTTGTGATTGTTGTGTGGCTATTGATTCAATGATTGTGTTGATTGTTGTTATAAGGATTACATCTTTTTTGCTATTACTAAGCTTTTTTTTTGTCTATTTTACTTTTAGCACTATCAGAGAAAGAGAGCATCAGAAGAAGAAAGCACAAGTCTTCCTGTGGACTGA

Protein sequence

>MS.gene57162.t1

MDRVFGRRFHLHPPRLLLRRLLRRYVDPAVADAADGPTLPIRASDEFRPFVRRLPEFKFWYSITVAFCIAIVMTFFSAFDIPVFWPILLFYWVVLFSLTMRRQISHMIKYRYVPFNFGKQHYQRKRASEEESTSLPVD