AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene57160


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57160.t1 MTR_5g016560 93.443 122 8 0 17 138 87 208 4.45e-79 232
MS.gene57160.t1 MTR_5g016560 92.308 104 8 0 17 120 87 190 9.48e-65 196
MS.gene57160.t1 MTR_4g094930 78.151 119 25 1 17 135 72 189 3.56e-62 189
MS.gene57160.t1 MTR_4g070400 68.868 106 33 0 17 122 76 181 1.97e-48 154
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57160.t1 AT4G39220 65.517 116 40 0 17 132 75 190 1.94e-54 169
MS.gene57160.t1 AT4G39220 65.517 116 40 0 17 132 75 190 1.94e-54 169
MS.gene57160.t1 AT2G21600 62.295 122 45 1 17 138 75 195 5.16e-52 163
MS.gene57160.t1 AT2G23310 67.925 106 33 1 27 132 107 211 7.82e-46 148
MS.gene57160.t1 AT2G23310 67.925 106 32 2 27 132 107 210 8.43e-44 143
MS.gene57160.t1 AT2G18240 62.136 103 38 1 17 119 79 180 1.28e-37 127
MS.gene57160.t1 AT2G18240 62.136 103 38 1 17 119 79 180 1.31e-37 127

Find 35 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCCTATACTCCTCTTCTAC+TGG 0.286487 5.2:+8283429 MS.gene57160:CDS
CGTTTGATATTCCAGTCTTC+TGG 0.311008 5.2:+8283408 MS.gene57160:CDS
AGATATGTACCGTTCAATTT+CGG 0.357992 5.2:+8283506 MS.gene57160:CDS
ACCGTTCAATTTCGGAAAAC+AGG 0.388365 5.2:+8283514 MS.gene57160:CDS
CTCCGCGTCTACTTCTTAGA+AGG 0.426734 5.2:+8281962 MS.gene57160:CDS
AACTCATCGGAAGCTCTAAT+AGG 0.427663 5.2:-8282097 None:intergenic
GCCTCCCTGAGTTCAAGTTC+TGG 0.451201 5.2:+8282137 MS.gene57160:CDS
TCAGCGGCGTCGGCGACGGC+GGG 0.451738 5.2:-8282061 None:intergenic
AGGAGTATAGGCCAGAAGAC+TGG 0.477362 5.2:-8283419 None:intergenic
GTCAGCGGCGTCGGCGACGG+CGG 0.477787 5.2:-8282062 None:intergenic
ACCGTCAGCGGCGTCGGCGA+CGG 0.481145 5.2:-8282065 None:intergenic
GCCGTCGCCGACGCCGCTGA+CGG 0.482016 5.2:+8282064 MS.gene57160:CDS
GCCTATACTCCTCTTCTACT+GGG 0.482573 5.2:+8283430 MS.gene57160:CDS
AACTGAAAATTTCAGTCCAC+AGG 0.507776 5.2:-8283712 None:intergenic
ACCCAGTAGAAGAGGAGTAT+AGG 0.512859 5.2:-8283431 None:intergenic
AGTAGGACCGTCAGCGGCGT+CGG 0.515054 5.2:-8282071 None:intergenic
TATTACCAGAACTTGAACTC+AGG 0.516687 5.2:-8282142 None:intergenic
TCAGGGAGGCGGCGAACGAA+AGG 0.517684 5.2:-8282124 None:intergenic
ACAAGACCACCCAGTAGAAG+AGG 0.526419 5.2:-8283439 None:intergenic
TACGTGCTACCGCGATGGAT+AGG 0.536752 5.2:+8281911 None:intergenic
TCTTGTTCTCGCTTACTATG+AGG 0.536784 5.2:+8283456 MS.gene57160:CDS
ACGTGCTACCGCGATGGATA+GGG 0.540998 5.2:+8281912 None:intergenic
GGAGGCGGCGAACGAAAGGA+CGG 0.592422 5.2:-8282120 None:intergenic
TGTTCTCGCTTACTATGAGG+AGG 0.593117 5.2:+8283459 MS.gene57160:CDS
ATTACCAGAACTTGAACTCA+GGG 0.599316 5.2:-8282141 None:intergenic
GAAGCTCTAATAGGAAGAGT+AGG 0.606900 5.2:-8282088 None:intergenic
AGGAAGAGTAGGACCGTCAG+CGG 0.634957 5.2:-8282077 None:intergenic
AGAAAGCACAAGTCTTCCTG+TGG 0.635231 5.2:+8283696 MS.gene57160:CDS
AGAACTTGAACTCAGGGAGG+CGG 0.636334 5.2:-8282135 None:intergenic
AACGAAAGGACGGAACTCAT+CGG 0.637257 5.2:-8282110 None:intergenic
TATACTCCTCTTCTACTGGG+TGG 0.667420 5.2:+8283433 MS.gene57160:CDS
AGCCTTCTAAGAAGTAGACG+CGG 0.688638 5.2:-8281964 None:intergenic
CTTCTAAGAAGTAGACGCGG+AGG 0.701782 5.2:-8281961 None:intergenic
ACCAGAACTTGAACTCAGGG+AGG 0.720457 5.2:-8282138 None:intergenic
GGGCAGGTACTCGATCACAG+TGG 0.793101 5.2:+8283349 MS.gene57160:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGTTTTTCATAAATTATAT+TGG - chr5.2:8283036-8283055 None:intergenic 10.0%
!! AAATTATATTGGATAGCTTA+TGG - chr5.2:8283025-8283044 None:intergenic 20.0%
!! TGCATAATTATTGTTAAATG+TGG + chr5.2:8282349-8282368 MS.gene57160:intron 20.0%
!!! ATCGTTTAATTGATTGATTT+TGG + chr5.2:8282185-8282204 MS.gene57160:intron 20.0%
!!! TAATTGATTGATTTTGGTAT+AGG + chr5.2:8282191-8282210 MS.gene57160:intron 20.0%
!!! TTCTCATCAAAATTTCTTTT+TGG - chr5.2:8282460-8282479 None:intergenic 20.0%
! AATTATGCACAATATCATCA+AGG - chr5.2:8282338-8282357 None:intergenic 25.0%
! ATTAAGTTCTTTATCCAAAG+AGG + chr5.2:8283155-8283174 MS.gene57160:intron 25.0%
! TATGGAAACAACTTATGATA+GGG + chr5.2:8282975-8282994 MS.gene57160:intron 25.0%
! TTACATATGTTGACATACAA+TGG - chr5.2:8282648-8282667 None:intergenic 25.0%
! TTATGGAAACAACTTATGAT+AGG + chr5.2:8282974-8282993 MS.gene57160:intron 25.0%
! TTTGTTAGATAGTTGTTAGT+TGG + chr5.2:8282599-8282618 MS.gene57160:intron 25.0%
!!! AGTTTCTTTTTCACTTAGTA+AGG + chr5.2:8283281-8283300 MS.gene57160:intron 25.0%
!!! TTGTGTTGATTGTTGTTATA+AGG + chr5.2:8283591-8283610 MS.gene57160:intron 25.0%
AGATATGTACCGTTCAATTT+CGG + chr5.2:8283506-8283525 MS.gene57160:CDS 30.0%
CCTAAAACAACATATCAATG+TGG - chr5.2:8283198-8283217 None:intergenic 30.0%
GTATATGATGAGAGTTTACT+GGG + chr5.2:8283128-8283147 MS.gene57160:intron 30.0%
TTAGATGATGTTGTCTTCTT+CGG + chr5.2:8283235-8283254 MS.gene57160:intron 30.0%
! TTCTCTTCATTTTCTTCACA+GGG + chr5.2:8282740-8282759 MS.gene57160:intron 30.0%
!!! CCACATTGATATGTTGTTTT+AGG + chr5.2:8283195-8283214 MS.gene57160:intron 30.0%
ACGAAGTGTGTATAAACTGA+CGG - chr5.2:8282281-8282300 None:intergenic 35.0%
ATGAAGAGAACCATAGAATG+TGG - chr5.2:8282730-8282749 None:intergenic 35.0%
ATTACCAGAACTTGAACTCA+GGG - chr5.2:8282144-8282163 None:intergenic 35.0%
CGTATATGATGAGAGTTTAC+TGG + chr5.2:8283127-8283146 MS.gene57160:intron 35.0%
GAAGACAACATCATCTAAGT+AGG - chr5.2:8283233-8283252 None:intergenic 35.0%
TACATCCTCAATCTTCTCAT+CGG + chr5.2:8282016-8282035 MS.gene57160:intron 35.0%
TATTACCAGAACTTGAACTC+AGG - chr5.2:8282145-8282164 None:intergenic 35.0%
TCAAATAAACCAACCCAAAC+AGG - chr5.2:8282766-8282785 None:intergenic 35.0%
TGACGGTAATGAATGATTGA+CGG - chr5.2:8282264-8282283 None:intergenic 35.0%
! ACCTGTTTTCCGAAATTGAA+CGG - chr5.2:8283518-8283537 None:intergenic 35.0%
! AGCTGCTCAATTTTCTTTTG+TGG + chr5.2:8283328-8283347 MS.gene57160:intron 35.0%
! CAATCTAGCTTGCAATATCA+AGG - chr5.2:8282393-8282412 None:intergenic 35.0%
! GCTGCTCAATTTTCTTTTGT+GGG + chr5.2:8283329-8283348 MS.gene57160:intron 35.0%
! GTTCTCTTCATTTTCTTCAC+AGG + chr5.2:8282739-8282758 MS.gene57160:intron 35.0%
! TTGTTGTTGTGATTGTTGTG+TGG + chr5.2:8283553-8283572 MS.gene57160:intron 35.0%
AATGTGGAAGACCCAAACTA+TGG - chr5.2:8283182-8283201 None:intergenic 40.0%
ACCGTTCAATTTCGGAAAAC+AGG + chr5.2:8283514-8283533 MS.gene57160:CDS 40.0%
ACTTCGTTCTCGTGTGATTA+AGG + chr5.2:8282294-8282313 MS.gene57160:intron 40.0%
AGAACCATAGAATGTGGTTG+AGG - chr5.2:8282724-8282743 None:intergenic 40.0%
AGACTGTTTCAGAGAGCTTA+TGG + chr5.2:8282957-8282976 MS.gene57160:intron 40.0%
ATCCAAAGAGGACCATAGTT+TGG + chr5.2:8283167-8283186 MS.gene57160:intron 40.0%
CGTTTGATATTCCAGTCTTC+TGG + chr5.2:8283408-8283427 MS.gene57160:CDS 40.0%
TCCAAAGAGGACCATAGTTT+GGG + chr5.2:8283168-8283187 MS.gene57160:intron 40.0%
TCTTGTTCTCGCTTACTATG+AGG + chr5.2:8283456-8283475 MS.gene57160:CDS 40.0%
! AACTCATCGGAAGCTCTAAT+AGG - chr5.2:8282100-8282119 None:intergenic 40.0%
! CTCAATTTTCTTTTGTGGGC+AGG + chr5.2:8283333-8283352 MS.gene57160:intron 40.0%
! GAAGCTCTAATAGGAAGAGT+AGG - chr5.2:8282091-8282110 None:intergenic 40.0%
! GATGTTGTCTTCTTCGGTTT+TGG + chr5.2:8283241-8283260 MS.gene57160:intron 40.0%
AACGAAAGGACGGAACTCAT+CGG - chr5.2:8282113-8282132 None:intergenic 45.0%
ACCCAAACTATGGTCCTCTT+TGG - chr5.2:8283172-8283191 None:intergenic 45.0%
CACTTAGTAAGGTCTGCAGA+AGG + chr5.2:8283292-8283311 MS.gene57160:intron 45.0%
GAGTCCTCAACCACATTCTA+TGG + chr5.2:8282717-8282736 MS.gene57160:intron 45.0%
GCCTATACTCCTCTTCTACT+GGG + chr5.2:8283430-8283449 MS.gene57160:CDS 45.0%
GGAAACCGATGAGAAGATTG+AGG - chr5.2:8282024-8282043 None:intergenic 45.0%
TATACTCCTCTTCTACTGGG+TGG + chr5.2:8283433-8283452 MS.gene57160:CDS 45.0%
TGCAAGCTAGATTGTGCATG+CGG + chr5.2:8282399-8282418 MS.gene57160:intron 45.0%
TGTTCTCGCTTACTATGAGG+AGG + chr5.2:8283459-8283478 MS.gene57160:CDS 45.0%
TTTCTTCACAGGGCCTGTTT+GGG + chr5.2:8282750-8282769 MS.gene57160:intron 45.0%
! ACCCAGTAGAAGAGGAGTAT+AGG - chr5.2:8283434-8283453 None:intergenic 45.0%
! AGAAAGCACAAGTCTTCCTG+TGG + chr5.2:8283696-8283715 MS.gene57160:CDS 45.0%
! AGCCTTCTAAGAAGTAGACG+CGG - chr5.2:8281967-8281986 None:intergenic 45.0%
! TTTTCTTCACAGGGCCTGTT+TGG + chr5.2:8282749-8282768 MS.gene57160:intron 45.0%
ACAAGACCACCCAGTAGAAG+AGG - chr5.2:8283442-8283461 None:intergenic 50.0%
ACCAGAACTTGAACTCAGGG+AGG - chr5.2:8282141-8282160 None:intergenic 50.0%
AGAACTTGAACTCAGGGAGG+CGG - chr5.2:8282138-8282157 None:intergenic 50.0%
CTCCGCGTCTACTTCTTAGA+AGG + chr5.2:8281962-8281981 MS.gene57160:CDS 50.0%
GGCCTATACTCCTCTTCTAC+TGG + chr5.2:8283429-8283448 MS.gene57160:CDS 50.0%
GGCTTCTACGTCGTTACGTA+CGG + chr5.2:8281983-8282002 MS.gene57160:intron 50.0%
GTGGTTGAGGACTCAGAAAC+TGG - chr5.2:8282711-8282730 None:intergenic 50.0%
TACGTCGTTACGTACGGTCT+TGG + chr5.2:8281989-8282008 MS.gene57160:intron 50.0%
! AGGAGTATAGGCCAGAAGAC+TGG - chr5.2:8283422-8283441 None:intergenic 50.0%
! CTTCTAAGAAGTAGACGCGG+AGG - chr5.2:8281964-8281983 None:intergenic 50.0%
AGGAAGAGTAGGACCGTCAG+CGG - chr5.2:8282080-8282099 None:intergenic 55.0%
TTCACAGGGCCTGTTTGGGT+TGG + chr5.2:8282754-8282773 MS.gene57160:intron 55.0%
! GCCTCCCTGAGTTCAAGTTC+TGG + chr5.2:8282137-8282156 MS.gene57160:CDS 55.0%
GGGCAGGTACTCGATCACAG+TGG + chr5.2:8283349-8283368 MS.gene57160:intron 60.0%
!! CATCGGTTTCCTCTCCCCTC+AGG + chr5.2:8282033-8282052 MS.gene57160:intron 60.0%
AGTAGGACCGTCAGCGGCGT+CGG - chr5.2:8282074-8282093 None:intergenic 65.0%
CGACGGCGGGATCAACCTGA+GGG - chr5.2:8282051-8282070 None:intergenic 65.0%
CGGGATCAACCTGAGGGGAG+AGG - chr5.2:8282045-8282064 None:intergenic 65.0%
GACGGCGGGATCAACCTGAG+GGG - chr5.2:8282050-8282069 None:intergenic 65.0%
GGAGGCGGCGAACGAAAGGA+CGG - chr5.2:8282123-8282142 None:intergenic 65.0%
TCAGGGAGGCGGCGAACGAA+AGG - chr5.2:8282127-8282146 None:intergenic 65.0%
GCGACGGCGGGATCAACCTG+AGG - chr5.2:8282052-8282071 None:intergenic 70.0%
!! ACCGTCAGCGGCGTCGGCGA+CGG - chr5.2:8282068-8282087 None:intergenic 75.0%
! GCCGTCGCCGACGCCGCTGA+CGG + chr5.2:8282064-8282083 MS.gene57160:CDS 80.0%
!!! GTCAGCGGCGTCGGCGACGG+CGG - chr5.2:8282065-8282084 None:intergenic 80.0%
!!! TCAGCGGCGTCGGCGACGGC+GGG - chr5.2:8282064-8282083 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 8281925 8283723 8281925 ID=MS.gene57160
chr5.2 mRNA 8281925 8283723 8281925 ID=MS.gene57160.t1;Parent=MS.gene57160
chr5.2 exon 8281925 8281999 8281925 ID=MS.gene57160.t1.exon1;Parent=MS.gene57160.t1
chr5.2 CDS 8281925 8281999 8281925 ID=cds.MS.gene57160.t1;Parent=MS.gene57160.t1
chr5.2 exon 8282055 8282158 8282055 ID=MS.gene57160.t1.exon2;Parent=MS.gene57160.t1
chr5.2 CDS 8282055 8282158 8282055 ID=cds.MS.gene57160.t1;Parent=MS.gene57160.t1
chr5.2 exon 8283355 8283535 8283355 ID=MS.gene57160.t1.exon3;Parent=MS.gene57160.t1
chr5.2 CDS 8283355 8283535 8283355 ID=cds.MS.gene57160.t1;Parent=MS.gene57160.t1
chr5.2 exon 8283667 8283723 8283667 ID=MS.gene57160.t1.exon4;Parent=MS.gene57160.t1
chr5.2 CDS 8283667 8283723 8283667 ID=cds.MS.gene57160.t1;Parent=MS.gene57160.t1
Gene Sequence

>MS.gene57160

ATGGATAGGGTTTTCGGTCGTCGCTTTCATCTACATCCTCCGCGTCTACTTCTTAGAAGGCTTCTACGTCGTTACGTACGGTCTTGGCATTTACATCCTCAATCTTCTCATCGGTTTCCTCTCCCCTCAGGTTGATCCCGCCGTCGCCGACGCCGCTGACGGTCCTACTCTTCCTATTAGAGCTTCCGATGAGTTCCGTCCTTTCGTTCGCCGCCTCCCTGAGTTCAAGTTCTGGTAATACTTCAATTTGATTATCAATTATCGTTTAATTGATTGATTTTGGTATAGGATTTAATTCATATACACTGTCAGTGTAAAGAGTTATGAGTTTTTACACCGTCAATCATTCATTACCGTCAGTTTATACACACTTCGTTCTCGTGTGATTAAGGTTATAGTTGCGTTATGATCCTTGATGATATTGTGCATAATTATTGTTAAATGTGGTTGATAATTGATGTTGTACCTTGATATTGCAAGCTAGATTGTGCATGCGGACATTCATTTAAAACGTTCTATGTGATGATGAGTTCCAAAAAGAAATTTTGATGAGAAGAATGTTTTGCTGTTAGTTTCTTATTATGATCTTTGTCGATGTATCGAAAACAATAATCATGTAATGTACTTCATTGCATGAAGCTATTGTTGAATCACAGAGTTAAGTTAATTATAATTTTGTTAGATAGTTGTTAGTTGGTTATGCTAGCAATCTGCTAACTACCATTGTATGTCAACATATGTAAGCTTATTGTTCTTGTATTCTTTTAACATTATTGAGTTGAACCAGTTTCTGAGTCCTCAACCACATTCTATGGTTCTCTTCATTTTCTTCACAGGGCCTGTTTGGGTTGGTTTATTTGAGTTTATCTATTAGCATCAACACATAAGCTTATTGTTGTCGTATTCTTTTAACGTTATTGAGTTGAATCAGTTTCTGAGTCCTCAACCACATTCTATGGTTCTCTTCATTTTCTTCACAGGGCCTGTTTGGGTTGGTTTATTTGAGTTTATCTATTAGCATTAACACACATGAGACTGTTTCAGAGAGCTTATGGAAACAACTTATGATAGGGCTATAATTTGTTTTCAGCTTATTTCCATAAGCTATCCAATATAATTTATGAAAAACAACTTATAGCTTATATGTAAACATTTTGATATTATCTTTTGTTAATGAAAGATTAACAACTTATACATAAGCACGTATATGATGAGAGTTTACTGGGTTTAATTAAGTTCTTTATCCAAAGAGGACCATAGTTTGGGTCTTCCACATTGATATGTTGTTTTAGGCATAAAAAAGTGCCTACTTAGATGATGTTGTCTTCTTCGGTTTTGGTGCTCTTAAGTTGTTGAAGTTTCTTTTTCACTTAGTAAGGTCTGCAGAAGGTTGCTTTTACTAAAGCTGCTCAATTTTCTTTTGTGGGCAGGTACTCGATCACAGTGGCATTTTGCATTGCAATTGTGATGACTTTCTTTAGTGCGTTTGATATTCCAGTCTTCTGGCCTATACTCCTCTTCTACTGGGTGGTCTTGTTCTCGCTTACTATGAGGAGGCAGATATCACATATGATCAAATACAGATATGTACCGTTCAATTTCGGAAAACAGGTAATTTAGATTCAAAATTGTTGTTGTGATTGTTGTGTGGCTATTGATTCAATGATTGTGTTGATTGTTGTTATAAGGATTACATCTTTTTTGCTATTACTAAGCTTTTTTTTTGTCTATTTTACTTTTAGCACTATCAGAGAAAGAGAGCATCAGAAGAAGAAAGCACAAGTCTTCCTGTGGACTGA

Protein sequence

>MS.gene57160.t1

MDRVFGRRFHLHPPRLLLRRLLRRYVDPAVADAADGPTLPIRASDEFRPFVRRLPEFKFWYSITVAFCIAIVMTFFSAFDIPVFWPILLFYWVVLFSLTMRRQISHMIKYRYVPFNFGKQHYQRKRASEEESTSLPVD