Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45193.t1 | XP_003613479.1 | 62.7 | 67 | 24 | 1 | 1 | 66 | 45 | 111 | 5.50E-12 | 79.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45193.t1 | G7K705 | 62.7 | 67 | 24 | 1 | 1 | 66 | 45 | 111 | 4.0e-12 | 79.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene45193 | 0.803898 | 2.80E-49 | -1.69E-46 |
| MS.gene049159 | MS.gene45193 | 0.827344 | 1.65E-54 | -1.69E-46 |
| MS.gene049272 | MS.gene45193 | 0.811657 | 6.27E-51 | -1.69E-46 |
| MS.gene049435 | MS.gene45193 | 0.833918 | 4.05E-56 | -1.69E-46 |
| MS.gene049501 | MS.gene45193 | 0.823471 | 1.36E-53 | -1.69E-46 |
| MS.gene049647 | MS.gene45193 | 0.806843 | 6.76E-50 | -1.69E-46 |
| MS.gene049850 | MS.gene45193 | 0.81848 | 1.92E-52 | -1.69E-46 |
| MS.gene049919 | MS.gene45193 | 0.804159 | 2.47E-49 | -1.69E-46 |
| MS.gene050020 | MS.gene45193 | 0.826696 | 2.35E-54 | -1.69E-46 |
| MS.gene050156 | MS.gene45193 | 0.81433 | 1.63E-51 | -1.69E-46 |
| MS.gene05016 | MS.gene45193 | 0.81445 | 1.53E-51 | -1.69E-46 |
| MS.gene050226 | MS.gene45193 | 0.804468 | 2.13E-49 | -1.69E-46 |
| MS.gene050442 | MS.gene45193 | 0.850421 | 1.72E-60 | -1.69E-46 |
| MS.gene050586 | MS.gene45193 | 0.801378 | 9.26E-49 | -1.69E-46 |
| MS.gene050629 | MS.gene45193 | -0.816192 | 6.28E-52 | -1.69E-46 |
| MS.gene050680 | MS.gene45193 | 0.826478 | 2.65E-54 | -1.69E-46 |
| MS.gene050719 | MS.gene45193 | 0.80706 | 6.08E-50 | -1.69E-46 |
| MS.gene050721 | MS.gene45193 | 0.804808 | 1.81E-49 | -1.69E-46 |
| MS.gene050724 | MS.gene45193 | 0.810474 | 1.13E-50 | -1.69E-46 |
| MS.gene050762 | MS.gene45193 | 0.82353 | 1.32E-53 | -1.69E-46 |
| MS.gene050763 | MS.gene45193 | 0.825365 | 4.88E-54 | -1.69E-46 |
| MS.gene050787 | MS.gene45193 | 0.848891 | 4.59E-60 | -1.69E-46 |
| MS.gene050807 | MS.gene45193 | 0.856348 | 3.42E-62 | -1.69E-46 |
| MS.gene050808 | MS.gene45193 | 0.815087 | 1.11E-51 | -1.69E-46 |
| MS.gene050815 | MS.gene45193 | 0.827455 | 1.55E-54 | -1.69E-46 |
| MS.gene050826 | MS.gene45193 | 0.84886 | 4.68E-60 | -1.69E-46 |
| MS.gene050844 | MS.gene45193 | 0.824416 | 8.17E-54 | -1.69E-46 |
| MS.gene050959 | MS.gene45193 | 0.837463 | 5.12E-57 | -1.69E-46 |
| MS.gene051147 | MS.gene45193 | 0.800264 | 1.56E-48 | -1.69E-46 |
| MS.gene051180 | MS.gene45193 | 0.845474 | 3.97E-59 | -1.69E-46 |
| MS.gene051210 | MS.gene45193 | 0.815497 | 8.97E-52 | -1.69E-46 |
| MS.gene051247 | MS.gene45193 | 0.813139 | 2.98E-51 | -1.69E-46 |
| MS.gene051265 | MS.gene45193 | 0.851006 | 1.18E-60 | -1.69E-46 |
| MS.gene051344 | MS.gene45193 | 0.815319 | 9.82E-52 | -1.69E-46 |
| MS.gene051388 | MS.gene45193 | 0.800378 | 1.48E-48 | -1.69E-46 |
| MS.gene051719 | MS.gene45193 | 0.829226 | 5.80E-55 | -1.69E-46 |
| MS.gene051720 | MS.gene45193 | 0.820055 | 8.40E-53 | -1.69E-46 |
| MS.gene051800 | MS.gene45193 | 0.807931 | 3.97E-50 | -1.69E-46 |
| MS.gene052020 | MS.gene45193 | 0.83171 | 1.43E-55 | -1.69E-46 |
| MS.gene052042 | MS.gene45193 | 0.803154 | 3.99E-49 | -1.69E-46 |
| MS.gene052176 | MS.gene45193 | 0.801391 | 9.21E-49 | -1.69E-46 |
| MS.gene052190 | MS.gene45193 | 0.815325 | 9.79E-52 | -1.69E-46 |
| MS.gene052323 | MS.gene45193 | 0.811095 | 8.31E-51 | -1.69E-46 |
| MS.gene052384 | MS.gene45193 | 0.808568 | 2.91E-50 | -1.69E-46 |
| MS.gene052516 | MS.gene45193 | 0.819423 | 1.17E-52 | -1.69E-46 |
| MS.gene052519 | MS.gene45193 | 0.810387 | 1.18E-50 | -1.69E-46 |
| MS.gene052611 | MS.gene45193 | 0.826805 | 2.22E-54 | -1.69E-46 |
| MS.gene052613 | MS.gene45193 | 0.819686 | 1.02E-52 | -1.69E-46 |
| MS.gene052665 | MS.gene45193 | 0.832141 | 1.12E-55 | -1.69E-46 |
| MS.gene052773 | MS.gene45193 | 0.822562 | 2.22E-53 | -1.69E-46 |
| MS.gene052863 | MS.gene45193 | 0.859536 | 3.87E-63 | -1.69E-46 |
| MS.gene052896 | MS.gene45193 | 0.854299 | 1.35E-61 | -1.69E-46 |
| MS.gene053008 | MS.gene45193 | 0.839639 | 1.40E-57 | -1.69E-46 |
| MS.gene053166 | MS.gene45193 | 0.845626 | 3.61E-59 | -1.69E-46 |
| MS.gene053184 | MS.gene45193 | 0.818211 | 2.21E-52 | -1.69E-46 |
| MS.gene053221 | MS.gene45193 | 0.812987 | 3.22E-51 | -1.69E-46 |
| MS.gene053331 | MS.gene45193 | 0.843879 | 1.07E-58 | -1.69E-46 |
| MS.gene053565 | MS.gene45193 | 0.808526 | 2.97E-50 | -1.69E-46 |
| MS.gene053811 | MS.gene45193 | 0.801218 | 9.99E-49 | -1.69E-46 |
| MS.gene05415 | MS.gene45193 | 0.803173 | 3.96E-49 | -1.69E-46 |
| MS.gene054236 | MS.gene45193 | 0.810068 | 1.39E-50 | -1.69E-46 |
| MS.gene054291 | MS.gene45193 | -0.821134 | 4.74E-53 | -1.69E-46 |
| MS.gene054379 | MS.gene45193 | 0.833147 | 6.30E-56 | -1.69E-46 |
| MS.gene054433 | MS.gene45193 | 0.807375 | 5.21E-50 | -1.69E-46 |
| MS.gene054467 | MS.gene45193 | 0.861503 | 9.80E-64 | -1.69E-46 |
| MS.gene054468 | MS.gene45193 | 0.867757 | 1.08E-65 | -1.69E-46 |
| MS.gene054472 | MS.gene45193 | 0.840614 | 7.82E-58 | -1.69E-46 |
| MS.gene054559 | MS.gene45193 | 0.818558 | 1.84E-52 | -1.69E-46 |
| MS.gene05462 | MS.gene45193 | 0.815939 | 7.15E-52 | -1.69E-46 |
| MS.gene05468 | MS.gene45193 | 0.824358 | 8.43E-54 | -1.69E-46 |
| MS.gene054775 | MS.gene45193 | 0.807135 | 5.86E-50 | -1.69E-46 |
| MS.gene054833 | MS.gene45193 | 0.805732 | 1.16E-49 | -1.69E-46 |
| MS.gene05510 | MS.gene45193 | 0.813608 | 2.35E-51 | -1.69E-46 |
| MS.gene055227 | MS.gene45193 | 0.823579 | 1.28E-53 | -1.69E-46 |
| MS.gene055356 | MS.gene45193 | 0.868215 | 7.70E-66 | -1.69E-46 |
| MS.gene055357 | MS.gene45193 | 0.827934 | 1.19E-54 | -1.69E-46 |
| MS.gene055358 | MS.gene45193 | 0.86287 | 3.73E-64 | -1.69E-46 |
| MS.gene055363 | MS.gene45193 | 0.827218 | 1.77E-54 | -1.69E-46 |
| MS.gene055465 | MS.gene45193 | 0.823175 | 1.60E-53 | -1.69E-46 |
| MS.gene055466 | MS.gene45193 | 0.824709 | 6.97E-54 | -1.69E-46 |
| MS.gene055468 | MS.gene45193 | 0.815087 | 1.11E-51 | -1.69E-46 |
| MS.gene055512 | MS.gene45193 | 0.815935 | 7.16E-52 | -1.69E-46 |
| MS.gene055608 | MS.gene45193 | 0.809413 | 1.92E-50 | -1.69E-46 |
| MS.gene055701 | MS.gene45193 | 0.826689 | 2.36E-54 | -1.69E-46 |
| MS.gene055778 | MS.gene45193 | 0.81493 | 1.20E-51 | -1.69E-46 |
| MS.gene055818 | MS.gene45193 | 0.857766 | 1.30E-62 | -1.69E-46 |
| MS.gene055926 | MS.gene45193 | 0.852793 | 3.66E-61 | -1.69E-46 |
| MS.gene05604 | MS.gene45193 | 0.863042 | 3.30E-64 | -1.69E-46 |
| MS.gene056069 | MS.gene45193 | -0.804891 | 1.74E-49 | -1.69E-46 |
| MS.gene056173 | MS.gene45193 | 0.827673 | 1.37E-54 | -1.69E-46 |
| MS.gene056506 | MS.gene45193 | 0.806026 | 1.00E-49 | -1.69E-46 |
| MS.gene056553 | MS.gene45193 | 0.801174 | 1.02E-48 | -1.69E-46 |
| MS.gene056589 | MS.gene45193 | 0.801278 | 9.71E-49 | -1.69E-46 |
| MS.gene056760 | MS.gene45193 | 0.800774 | 1.23E-48 | -1.69E-46 |
| MS.gene057026 | MS.gene45193 | 0.82602 | 3.41E-54 | -1.69E-46 |
| MS.gene057060 | MS.gene45193 | 0.808021 | 3.80E-50 | -1.69E-46 |
| MS.gene057194 | MS.gene45193 | 0.80326 | 3.79E-49 | -1.69E-46 |
| MS.gene057444 | MS.gene45193 | 0.80481 | 1.81E-49 | -1.69E-46 |
| MS.gene057469 | MS.gene45193 | 0.816258 | 6.07E-52 | -1.69E-46 |
| MS.gene057693 | MS.gene45193 | 0.808249 | 3.40E-50 | -1.69E-46 |
| MS.gene057869 | MS.gene45193 | 0.81744 | 3.29E-52 | -1.69E-46 |
| MS.gene057915 | MS.gene45193 | 0.837127 | 6.24E-57 | -1.69E-46 |
| MS.gene058146 | MS.gene45193 | 0.808735 | 2.68E-50 | -1.69E-46 |
| MS.gene058168 | MS.gene45193 | 0.812653 | 3.81E-51 | -1.69E-46 |
| MS.gene058399 | MS.gene45193 | 0.802399 | 5.72E-49 | -1.69E-46 |
| MS.gene058625 | MS.gene45193 | 0.839588 | 1.45E-57 | -1.69E-46 |
| MS.gene058636 | MS.gene45193 | 0.823102 | 1.66E-53 | -1.69E-46 |
| MS.gene058829 | MS.gene45193 | 0.820847 | 5.53E-53 | -1.69E-46 |
| MS.gene058979 | MS.gene45193 | 0.802676 | 5.01E-49 | -1.69E-46 |
| MS.gene059128 | MS.gene45193 | 0.841602 | 4.30E-58 | -1.69E-46 |
| MS.gene059161 | MS.gene45193 | 0.823947 | 1.05E-53 | -1.69E-46 |
| MS.gene059272 | MS.gene45193 | 0.811273 | 7.60E-51 | -1.69E-46 |
| MS.gene059561 | MS.gene45193 | 0.802281 | 6.04E-49 | -1.69E-46 |
| MS.gene059607 | MS.gene45193 | 0.807547 | 4.79E-50 | -1.69E-46 |
| MS.gene059713 | MS.gene45193 | 0.832645 | 8.40E-56 | -1.69E-46 |
| MS.gene059725 | MS.gene45193 | 0.824114 | 9.62E-54 | -1.69E-46 |
| MS.gene059789 | MS.gene45193 | 0.820824 | 5.59E-53 | -1.69E-46 |
| MS.gene059842 | MS.gene45193 | 0.807101 | 5.96E-50 | -1.69E-46 |
| MS.gene059937 | MS.gene45193 | 0.813895 | 2.03E-51 | -1.69E-46 |
| MS.gene060137 | MS.gene45193 | -0.826884 | 2.12E-54 | -1.69E-46 |
| MS.gene060167 | MS.gene45193 | 0.810602 | 1.06E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene45193 | 0.864026 | 1.64E-64 | -1.69E-46 |
| MS.gene06052 | MS.gene45193 | 0.82142 | 4.08E-53 | -1.69E-46 |
| MS.gene060683 | MS.gene45193 | 0.85078 | 1.36E-60 | -1.69E-46 |
| MS.gene060685 | MS.gene45193 | 0.85269 | 3.91E-61 | -1.69E-46 |
| MS.gene060802 | MS.gene45193 | 0.84 | 1.13E-57 | -1.69E-46 |
| MS.gene060803 | MS.gene45193 | 0.859839 | 3.13E-63 | -1.69E-46 |
| MS.gene060998 | MS.gene45193 | 0.842107 | 3.16E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45193.t1 | MTR_5g037140 | 98.507 | 67 | 0 | 1 | 1 | 66 | 45 | 111 | 3.31e-43 | 135 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 14 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAACAAACCTGTTGTGAAT+AGG | 0.400300 | 5.1:-27654261 | None:intergenic |
| TTAGTTAGTGGTGGAGGAAC+AGG | 0.433994 | 5.1:+27654090 | MS.gene45193:CDS |
| TTTAGTAGCTTAGTTAGTGG+TGG | 0.443725 | 5.1:+27654081 | MS.gene45193:CDS |
| TGTCAACCATGATGCACAAT+TGG | 0.454537 | 5.1:+27654194 | MS.gene45193:CDS |
| GACCAGATCCTATTCACAAC+AGG | 0.455835 | 5.1:+27654253 | MS.gene45193:CDS |
| CATGATGAACAAGTTGTTGT+TGG | 0.456308 | 5.1:+27654153 | MS.gene45193:CDS |
| TGAATAGGATCTGGTCCATT+GGG | 0.478116 | 5.1:-27654246 | None:intergenic |
| AGTAGCTTAGTTAGTGGTGG+AGG | 0.503163 | 5.1:+27654084 | MS.gene45193:CDS |
| AACCTGTTGTGAATAGGATC+TGG | 0.513984 | 5.1:-27654255 | None:intergenic |
| GTGAATAGGATCTGGTCCAT+TGG | 0.561168 | 5.1:-27654247 | None:intergenic |
| TGAAGTCCAATTGTGCATCA+TGG | 0.582511 | 5.1:-27654200 | None:intergenic |
| AGTTGTTGTTGGTAAAGACA+AGG | 0.593670 | 5.1:+27654164 | MS.gene45193:CDS |
| GACAAACAACTACAAGACAT+TGG | 0.623172 | 5.1:+27654112 | MS.gene45193:CDS |
| AACTACAAGACATTGGTCAG+AGG | 0.649811 | 5.1:+27654119 | MS.gene45193:CDS |
| GTTGTTGTTGGTAAAGACAA+GGG | 0.652393 | 5.1:+27654165 | MS.gene45193:CDS |
| AGTAAAAGAAGAGTTCCCAA+TGG | 0.653384 | 5.1:+27654231 | MS.gene45193:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTTTTTAGTAGCTTAGTTAG+TGG | + | chr5.1:27654078-27654097 | MS.gene45193:CDS | 30.0% |
| AGTAAAAGAAGAGTTCCCAA+TGG | + | chr5.1:27654231-27654250 | MS.gene45193:CDS | 35.0% | |
| CATGATGAACAAGTTGTTGT+TGG | + | chr5.1:27654153-27654172 | MS.gene45193:CDS | 35.0% | |
| GACAAACAACTACAAGACAT+TGG | + | chr5.1:27654112-27654131 | MS.gene45193:CDS | 35.0% | |
| TTTAGTAGCTTAGTTAGTGG+TGG | + | chr5.1:27654081-27654100 | MS.gene45193:CDS | 35.0% | |
| ! | AGTTGTTGTTGGTAAAGACA+AGG | + | chr5.1:27654164-27654183 | MS.gene45193:CDS | 35.0% |
| ! | GTTGTTGTTGGTAAAGACAA+GGG | + | chr5.1:27654165-27654184 | MS.gene45193:CDS | 35.0% |
| AACTACAAGACATTGGTCAG+AGG | + | chr5.1:27654119-27654138 | MS.gene45193:CDS | 40.0% | |
| TGAAGTCCAATTGTGCATCA+TGG | - | chr5.1:27654203-27654222 | None:intergenic | 40.0% | |
| TGAATAGGATCTGGTCCATT+GGG | - | chr5.1:27654249-27654268 | None:intergenic | 40.0% | |
| TGTCAACCATGATGCACAAT+TGG | + | chr5.1:27654194-27654213 | MS.gene45193:CDS | 40.0% | |
| AGTAGCTTAGTTAGTGGTGG+AGG | + | chr5.1:27654084-27654103 | MS.gene45193:CDS | 45.0% | |
| GTGAATAGGATCTGGTCCAT+TGG | - | chr5.1:27654250-27654269 | None:intergenic | 45.0% | |
| ! | TTAGTTAGTGGTGGAGGAAC+AGG | + | chr5.1:27654090-27654109 | MS.gene45193:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 27654075 | 27654274 | 27654075 | ID=MS.gene45193 |
| chr5.1 | mRNA | 27654075 | 27654274 | 27654075 | ID=MS.gene45193.t1;Parent=MS.gene45193 |
| chr5.1 | exon | 27654075 | 27654274 | 27654075 | ID=MS.gene45193.t1.exon1;Parent=MS.gene45193.t1 |
| chr5.1 | CDS | 27654075 | 27654274 | 27654075 | ID=cds.MS.gene45193.t1;Parent=MS.gene45193.t1 |
| Gene Sequence |
| Protein sequence |
