AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58945


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58945.t1 MTR_4g118800 92.593 189 9 2 1 186 1 187 6.26e-106 301
MS.gene58945.t1 MTR_7g108130 34.591 159 91 4 30 180 29 182 1.74e-24 94.7
MS.gene58945.t1 MTR_2g020060 33.735 166 93 3 32 183 25 187 1.30e-21 87.4
MS.gene58945.t1 MTR_4g118810 31.361 169 91 4 5 159 17 174 3.26e-19 81.3
MS.gene58945.t1 MTR_0004s0210 34.194 155 87 4 32 182 10 153 4.24e-18 77.4
MS.gene58945.t1 MTR_4g081880 30.247 162 100 5 28 180 27 184 9.55e-16 72.0
MS.gene58945.t1 MTR_8g089300 31.206 141 83 3 32 159 28 167 1.57e-15 71.6
MS.gene58945.t1 MTR_7g011090 31.098 164 102 6 28 183 26 186 2.01e-14 68.6
MS.gene58945.t1 MTR_5g041900 34.839 155 87 5 28 170 26 178 7.99e-14 66.6
MS.gene58945.t1 MTR_7g083120 29.936 157 101 4 5 153 2 157 3.69e-13 65.1
MS.gene58945.t1 MTR_2g026530 32.692 156 99 3 31 180 32 187 1.05e-11 61.2
MS.gene58945.t1 MTR_2g026530 32.692 156 99 3 31 180 12 167 1.41e-11 60.5
MS.gene58945.t1 MTR_0097s0070 32.203 118 75 4 61 176 71 185 5.75e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58945.t1 AT3G06390 47.399 173 74 3 1 156 1 173 2.51e-49 159
MS.gene58945.t1 AT4G15610 50.877 171 70 4 3 164 7 172 3.10e-49 158
MS.gene58945.t1 AT4G15610 62.766 94 35 0 71 164 23 116 1.21e-36 124
MS.gene58945.t1 AT4G15630 30.247 162 97 2 30 177 27 186 4.10e-20 83.6
MS.gene58945.t1 AT5G15290 31.618 136 84 4 31 159 29 162 8.50e-17 74.7
MS.gene58945.t1 AT4G15620 27.950 161 100 2 31 177 28 186 9.01e-17 74.7
MS.gene58945.t1 AT1G14160 29.487 156 102 4 28 177 49 202 1.67e-14 68.9
MS.gene58945.t1 AT2G36100 30.882 136 87 3 30 159 48 182 1.32e-12 63.9
MS.gene58945.t1 AT1G03700 28.758 153 97 3 32 180 11 155 1.24e-11 60.5

Find 45 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTGGGAGTGTTGCATATT+TGG 0.167909 8.3:+7875736 MS.gene58945:CDS
TTCTCCTCCATTTAATCTTC+TGG 0.173285 8.3:+7875569 MS.gene58945:CDS
GTGGCTGCATTCTCTGTTGC+TGG 0.248825 8.3:+7875478 MS.gene58945:CDS
TTGTCACTCTGTTGCTCATT+TGG 0.263577 8.3:+7875864 MS.gene58945:CDS
GGCTCCGGCTTCTTCGGCTT+CGG 0.290085 8.3:+7874427 MS.gene58945:CDS
CCGCATCCCAGAAGATTAAA+TGG 0.293656 8.3:-7875576 None:intergenic
GCTGGGAGTGTTGCATATTT+GGG 0.294630 8.3:+7875737 MS.gene58945:CDS
AGGGAAACAACCACACTAAT+TGG 0.296811 8.3:+7875765 MS.gene58945:CDS
GCTCCGGCTTCTTCGGCTTC+GGG 0.310874 8.3:+7874428 MS.gene58945:CDS
TCAGTTGTTGTGATGGTTAC+TGG 0.316039 8.3:+7874515 MS.gene58945:CDS
GATTAAATGGAGGAGAAGTT+TGG 0.346130 8.3:-7875563 None:intergenic
CAACTTTCTTCAAATCTACT+TGG 0.347310 8.3:-7875918 None:intergenic
TCTCCTCCATTTAATCTTCT+GGG 0.353459 8.3:+7875570 MS.gene58945:CDS
TTTCTCTTGCAGGTGATGTT+GGG 0.377570 8.3:+7875689 MS.gene58945:intron
GGGATATTAGCTTCAGCAAC+AGG 0.379873 8.3:+7875710 MS.gene58945:CDS
AACTCCGGCTCCGGCTTCTT+CGG 0.390199 8.3:+7874421 MS.gene58945:CDS
GCTTCAGCAACAGGCACAGC+TGG 0.420135 8.3:+7875719 MS.gene58945:CDS
TCTGTAGCAGTGGGATTGTT+TGG 0.423026 8.3:+7875836 MS.gene58945:CDS
TGGCTGCATTCTCTGTTGCT+GGG 0.437451 8.3:+7875479 MS.gene58945:CDS
ATCATCTTCAACTCCGGCTC+CGG 0.452738 8.3:+7874412 MS.gene58945:CDS
CTAAGTTTGATTTGATCCTA+AGG 0.461173 8.3:+7874465 MS.gene58945:CDS
TGTTGCATATTTGGGTTTGA+AGG 0.466255 8.3:+7875745 MS.gene58945:CDS
ATTTCTCTTGCAGGTGATGT+TGG 0.478178 8.3:+7875688 MS.gene58945:intron
AACTTTCTTCAAATCTACTT+GGG 0.490912 8.3:-7875917 None:intergenic
GTTGAAGATGATTTGTACTC+AGG 0.496735 8.3:-7874401 None:intergenic
TGCAAAACTTACACAAAGGC+TGG 0.513124 8.3:-7874581 None:intergenic
ACACCCGAAGCCGAAGAAGC+CGG 0.514862 8.3:-7874431 None:intergenic
TCTGTTGTTTGCAGCATCAG+TGG 0.515368 8.3:+7874490 MS.gene58945:CDS
GGCTGGTGAGTATCTAAACT+TGG 0.516960 8.3:-7874564 None:intergenic
GAGCAAGACATGGCTTCTAC+TGG 0.517836 8.3:+7874374 None:intergenic
GAAGCCGAAGAAGCCGGAGC+CGG 0.518970 8.3:-7874425 None:intergenic
GTTGCATATTTGGGTTTGAA+GGG 0.522790 8.3:+7875746 MS.gene58945:CDS
TTTGAGGTTTGGCTTTCGGA+TGG 0.533858 8.3:-7875539 None:intergenic
CTGCAAACAACAGAAACCTT+AGG 0.533947 8.3:-7874481 None:intergenic
GGTGAGTATCTAAACTTGGC+TGG 0.534764 8.3:-7874560 None:intergenic
TTCTCTTGCAGGTGATGTTG+GGG 0.535972 8.3:+7875690 MS.gene58945:intron
CCATTTAATCTTCTGGGATG+CGG 0.538436 8.3:+7875576 MS.gene58945:CDS
TATGGGTGCCTCTGTAGCAG+TGG 0.553144 8.3:+7875826 MS.gene58945:CDS
GTACAAATCATCTTCAACTC+CGG 0.560040 8.3:+7874406 MS.gene58945:CDS
AAACAATCCCACTGCTACAG+AGG 0.565188 8.3:-7875834 None:intergenic
ATGGGTGCCTCTGTAGCAGT+GGG 0.565933 8.3:+7875827 MS.gene58945:CDS
CTTCAGCAACAGGCACAGCT+GGG 0.583484 8.3:+7875720 MS.gene58945:CDS
AGTGGCATCAGTTGTTGTGA+TGG 0.612540 8.3:+7874508 MS.gene58945:CDS
TGCAAAACTTGTCATAGATG+TGG 0.673434 8.3:-7875799 None:intergenic
CATCCCAGAAGATTAAATGG+AGG 0.717011 8.3:-7875573 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAGAAATTATATAAAAAAA+TGG + chr8.3:7874936-7874955 MS.gene58945:intron 10.0%
!!! AATATAATAATTTTCACTAT+TGG - chr8.3:7875095-7875114 None:intergenic 10.0%
!!! ATTATATAAAAAAATGGATT+TGG + chr8.3:7874942-7874961 MS.gene58945:intron 10.0%
!! AATTAATATCAATGAGAATA+AGG - chr8.3:7875408-7875427 None:intergenic 15.0%
!! AAGGTAAAAATGGAATTAAA+AGG - chr8.3:7875621-7875640 None:intergenic 20.0%
!! ATAACTATTAAGCATCTAAT+TGG + chr8.3:7874660-7874679 MS.gene58945:intron 20.0%
!! ATCATTGGATTATGTTTAAA+AGG - chr8.3:7875670-7875689 None:intergenic 20.0%
!! GAAGAAAATTGTAACATAAA+TGG - chr8.3:7874989-7875008 None:intergenic 20.0%
!! TAGTTATTTGCAATATTCTT+GGG + chr8.3:7874725-7874744 MS.gene58945:intron 20.0%
!! TTAGTTATTTGCAATATTCT+TGG + chr8.3:7874724-7874743 MS.gene58945:intron 20.0%
!!! TTATAATCACAGATATTTTG+TGG + chr8.3:7875459-7875478 MS.gene58945:intron 20.0%
! AGTATTTAGCAAGGTAAAAA+TGG - chr8.3:7875631-7875650 None:intergenic 25.0%
! ATAACAAACATAAAACTCAG+CGG - chr8.3:7874687-7874706 None:intergenic 25.0%
! ATGAGAATAAGGTCTTAATA+TGG - chr8.3:7875397-7875416 None:intergenic 25.0%
! GAGTATAATAGTATTTAGCA+AGG - chr8.3:7875640-7875659 None:intergenic 25.0%
! TCATAATTTATGAGACACTA+AGG + chr8.3:7875031-7875050 MS.gene58945:intron 25.0%
! TTAATTAGGAGAAAGAAAAG+CGG - chr8.3:7875247-7875266 None:intergenic 25.0%
!! AAATTTTATGCCAATTAGTG+TGG - chr8.3:7875778-7875797 None:intergenic 25.0%
AGAATAAGGTCTTAATATGG+TGG - chr8.3:7875394-7875413 None:intergenic 30.0%
ATCATGCAAAACTTACACAA+AGG - chr8.3:7874588-7874607 None:intergenic 30.0%
GAATAAGGTCTTAATATGGT+GGG - chr8.3:7875393-7875412 None:intergenic 30.0%
GTATCAATCAAATGGAGAAT+TGG - chr8.3:7875225-7875244 None:intergenic 30.0%
! CTAGCTGATAGAATTAGTTT+CGG - chr8.3:7874837-7874856 None:intergenic 30.0%
!! CTAAGTTTGATTTGATCCTA+AGG + chr8.3:7874465-7874484 MS.gene58945:CDS 30.0%
AATCCAATGATTTCTCTTGC+AGG + chr8.3:7875679-7875698 MS.gene58945:intron 35.0%
ACATGACCATATTCCATAGT+AGG - chr8.3:7874764-7874783 None:intergenic 35.0%
AGACACTAAGGATATTGTAG+GGG + chr8.3:7875043-7875062 MS.gene58945:intron 35.0%
ATTCCATAGTAGGAACTACT+AGG - chr8.3:7874754-7874773 None:intergenic 35.0%
CAGCTAGACTCAATTTCAAT+AGG + chr8.3:7874850-7874869 MS.gene58945:intron 35.0%
GAAAAGCGGTATCAATCAAA+TGG - chr8.3:7875233-7875252 None:intergenic 35.0%
GAGACACTAAGGATATTGTA+GGG + chr8.3:7875042-7875061 MS.gene58945:intron 35.0%
GATTAAATGGAGGAGAAGTT+TGG - chr8.3:7875566-7875585 None:intergenic 35.0%
GTACAAATCATCTTCAACTC+CGG + chr8.3:7874406-7874425 MS.gene58945:CDS 35.0%
GTAGTTCCTACTATGGAATA+TGG + chr8.3:7874755-7874774 MS.gene58945:intron 35.0%
TCTCCTCCATTTAATCTTCT+GGG + chr8.3:7875570-7875589 MS.gene58945:CDS 35.0%
TGAGACACTAAGGATATTGT+AGG + chr8.3:7875041-7875060 MS.gene58945:intron 35.0%
TGCAAAACTTGTCATAGATG+TGG - chr8.3:7875802-7875821 None:intergenic 35.0%
TTCTCCTCCATTTAATCTTC+TGG + chr8.3:7875569-7875588 MS.gene58945:CDS 35.0%
TTTCCTAGTAGTTCCTACTA+TGG + chr8.3:7874748-7874767 MS.gene58945:intron 35.0%
! ACATCTATGACAAGTTTTGC+AGG + chr8.3:7875801-7875820 MS.gene58945:CDS 35.0%
! GAAAGACTCTACTCTTTTGA+AGG + chr8.3:7875315-7875334 MS.gene58945:intron 35.0%
! GTTGAAGATGATTTGTACTC+AGG - chr8.3:7874404-7874423 None:intergenic 35.0%
! GTTGCATATTTGGGTTTGAA+GGG + chr8.3:7875746-7875765 MS.gene58945:CDS 35.0%
! TAATATGGTGGGAACATATG+TGG - chr8.3:7875382-7875401 None:intergenic 35.0%
! TGTTGCATATTTGGGTTTGA+AGG + chr8.3:7875745-7875764 MS.gene58945:CDS 35.0%
! TTTGAAGTATGCATCTCTCT+CGG + chr8.3:7874783-7874802 MS.gene58945:intron 35.0%
!! CTACTCTTTTGAAGGCTATA+AGG + chr8.3:7875323-7875342 MS.gene58945:intron 35.0%
!!! AGAAGTTTGGTTTTGAGGTT+TGG - chr8.3:7875553-7875572 None:intergenic 35.0%
AGGGAAACAACCACACTAAT+TGG + chr8.3:7875765-7875784 MS.gene58945:CDS 40.0%
ATTTCTCTTGCAGGTGATGT+TGG + chr8.3:7875688-7875707 MS.gene58945:intron 40.0%
CATCCCAGAAGATTAAATGG+AGG - chr8.3:7875576-7875595 None:intergenic 40.0%
CTGCAAACAACAGAAACCTT+AGG - chr8.3:7874484-7874503 None:intergenic 40.0%
TCACCTGCAAGAGAAATCAT+TGG - chr8.3:7875685-7875704 None:intergenic 40.0%
TCAGTTGTTGTGATGGTTAC+TGG + chr8.3:7874515-7874534 MS.gene58945:CDS 40.0%
TGCAAAACTTACACAAAGGC+TGG - chr8.3:7874584-7874603 None:intergenic 40.0%
TTGTCACTCTGTTGCTCATT+TGG + chr8.3:7875864-7875883 MS.gene58945:CDS 40.0%
TTTCTCTTGCAGGTGATGTT+GGG + chr8.3:7875689-7875708 MS.gene58945:intron 40.0%
! GACAAGTTTTGCAGGCATAT+GGG + chr8.3:7875809-7875828 MS.gene58945:CDS 40.0%
! TGACAAGTTTTGCAGGCATA+TGG + chr8.3:7875808-7875827 MS.gene58945:CDS 40.0%
!! CCATTTAATCTTCTGGGATG+CGG + chr8.3:7875576-7875595 MS.gene58945:CDS 40.0%
!! CTCTTTTGAAGGCTATAAGG+AGG + chr8.3:7875326-7875345 MS.gene58945:intron 40.0%
!!! TGGTTTTGAGGTTTGGCTTT+CGG - chr8.3:7875546-7875565 None:intergenic 40.0%
AAACAATCCCACTGCTACAG+AGG - chr8.3:7875837-7875856 None:intergenic 45.0%
AGTGGCATCAGTTGTTGTGA+TGG + chr8.3:7874508-7874527 MS.gene58945:CDS 45.0%
CCGCATCCCAGAAGATTAAA+TGG - chr8.3:7875579-7875598 None:intergenic 45.0%
GGCTGGTGAGTATCTAAACT+TGG - chr8.3:7874567-7874586 None:intergenic 45.0%
GGTGAGTATCTAAACTTGGC+TGG - chr8.3:7874563-7874582 None:intergenic 45.0%
TCTGTAGCAGTGGGATTGTT+TGG + chr8.3:7875836-7875855 MS.gene58945:CDS 45.0%
TTCTCTTGCAGGTGATGTTG+GGG + chr8.3:7875690-7875709 MS.gene58945:intron 45.0%
! GGGATATTAGCTTCAGCAAC+AGG + chr8.3:7875710-7875729 MS.gene58945:CDS 45.0%
! TCTGTTGTTTGCAGCATCAG+TGG + chr8.3:7874490-7874509 MS.gene58945:CDS 45.0%
!! AGCTGGGAGTGTTGCATATT+TGG + chr8.3:7875736-7875755 MS.gene58945:CDS 45.0%
!! GCTGGGAGTGTTGCATATTT+GGG + chr8.3:7875737-7875756 MS.gene58945:CDS 45.0%
!! TTTGAGGTTTGGCTTTCGGA+TGG - chr8.3:7875542-7875561 None:intergenic 45.0%
!!! GGAGGAGAAGTTTGGTTTTG+AGG - chr8.3:7875558-7875577 None:intergenic 45.0%
!! TAAATAAATAAATGATATAT+TGG - chr8.3:7875180-7875199 None:intergenic 5.0%
!! TTTGTTTATTAAATTTAATT+AGG - chr8.3:7875261-7875280 None:intergenic 5.0%
ATCATCTTCAACTCCGGCTC+CGG + chr8.3:7874412-7874431 MS.gene58945:CDS 50.0%
TGGCTGCATTCTCTGTTGCT+GGG + chr8.3:7875479-7875498 MS.gene58945:CDS 50.0%
ATGGGTGCCTCTGTAGCAGT+GGG + chr8.3:7875827-7875846 MS.gene58945:CDS 55.0%
CTTCAGCAACAGGCACAGCT+GGG + chr8.3:7875720-7875739 MS.gene58945:CDS 55.0%
GTGGCTGCATTCTCTGTTGC+TGG + chr8.3:7875478-7875497 MS.gene58945:CDS 55.0%
TATGGGTGCCTCTGTAGCAG+TGG + chr8.3:7875826-7875845 MS.gene58945:CDS 55.0%
AACTCCGGCTCCGGCTTCTT+CGG + chr8.3:7874421-7874440 MS.gene58945:CDS 60.0%
ACACCCGAAGCCGAAGAAGC+CGG - chr8.3:7874434-7874453 None:intergenic 60.0%
GCTTCAGCAACAGGCACAGC+TGG + chr8.3:7875719-7875738 MS.gene58945:CDS 60.0%
GAAGCCGAAGAAGCCGGAGC+CGG - chr8.3:7874428-7874447 None:intergenic 65.0%
GCTCCGGCTTCTTCGGCTTC+GGG + chr8.3:7874428-7874447 MS.gene58945:CDS 65.0%
GGCTCCGGCTTCTTCGGCTT+CGG + chr8.3:7874427-7874446 MS.gene58945:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 7874383 7875925 7874383 ID=MS.gene58945
chr8.3 mRNA 7874383 7875925 7874383 ID=MS.gene58945.t1;Parent=MS.gene58945
chr8.3 exon 7874383 7874591 7874383 ID=MS.gene58945.t1.exon1;Parent=MS.gene58945.t1
chr8.3 CDS 7874383 7874591 7874383 ID=cds.MS.gene58945.t1;Parent=MS.gene58945.t1
chr8.3 exon 7875471 7875597 7875471 ID=MS.gene58945.t1.exon2;Parent=MS.gene58945.t1
chr8.3 CDS 7875471 7875597 7875471 ID=cds.MS.gene58945.t1;Parent=MS.gene58945.t1
chr8.3 exon 7875701 7875925 7875701 ID=MS.gene58945.t1.exon3;Parent=MS.gene58945.t1
chr8.3 CDS 7875701 7875925 7875701 ID=cds.MS.gene58945.t1;Parent=MS.gene58945.t1
Gene Sequence

>MS.gene58945

ATGGCTTCTACTGGTGATCCTGAGTACAAATCATCTTCAACTCCGGCTCCGGCTTCTTCGGCTTCGGGTGTTGTTGATCACTCTAAGTTTGATTTGATCCTAAGGTTTCTGTTGTTTGCAGCATCAGTGGCATCAGTTGTTGTGATGGTTACTGGTAATCAAACAGAATATTCTCGACCAGCCAAGTTTAGATACTCACCAGCCTTTGTGTAAGTTTTGCATGATAATATTTTTAGTTTTTCAAAATTCAAATCAATTCAGTAATTAGTTTTGCATGATAACTATTAAGCATCTAATTGGTCCGCTGAGTTTTATGTTTGTTATATATGTGTCTGCTTATTTTAGTTATTTGCAATATTCTTGGGTTTCCTAGTAGTTCCTACTATGGAATATGGTCATGTTTGAAGTATGCATCTCTCTCGGTTGAAATTCAAACTTTAATTCGACACGTCCGAAACTAATTCTATCAGCTAGACTCAATTTCAATAGGCAATTTACAAATCAGACACACACACAAACACAATATTATGTATGTATGTATGTATACGTGTGTGAAGAAATTATATAAAAAAATGGATTTGGATAATAAGCTTAACAACTTTTCCATTTATGTTACAATTTTCTTCTTTTTTTCTTGAATAAATTGTTTCATAATTTATGAGACACTAAGGATATTGTAGGGGTATGAAATTATATGTGCTTCTCTTTGCCAATAGTGAAAATTATTATATTTGTCTCATATTATGTGTGTTATTTAGAATATTTTTATGTTTCAAATTTGTAATTTTGCAATACCAATATATCATTTATTTATTTATTTATTTTTTAAATGTAAGAAGCCAATTCTCCATTTGATTGATACCGCTTTTCTTTCTCCTAATTAAATTTAATAAACAAATAAATATTTCAAGTTTTGCAAGTTGTGAACTAAAGAAAGACTCTACTCTTTTGAAGGCTATAAGGAGGCAAAGTCATCGATGATGACTTTGTTTAAGACCACATATGTTCCCACCATATTAAGACCTTATTCTCATTGATATTAATTGTTTTTTATCAACTTGCAATTAATTTGACATTTATAATCACAGATATTTTGTGGCTGCATTCTCTGTTGCTGGGCTTTATAGTATCATTACAACTTTTGTATCACTCTCTGCCATCCGAAAGCCAAACCTCAAAACCAAACTTCTCCTCCATTTAATCTTCTGGGATGCGGTAATTAATCAATCAATTAGCCTTTTAATTCCATTTTTACCTTGCTAAATACTATTATACTCATCTTCTCCTTTTAAACATAATCCAATGATTTCTCTTGCAGGTGATGTTGGGGATATTAGCTTCAGCAACAGGCACAGCTGGGAGTGTTGCATATTTGGGTTTGAAGGGAAACAACCACACTAATTGGCATAAAATTTGCCACATCTATGACAAGTTTTGCAGGCATATGGGTGCCTCTGTAGCAGTGGGATTGTTTGGTAGCATTGTCACTCTGTTGCTCATTTGGATTTCAGCTCACAGCATTCATAGTCGAGTTCCCAAGTAG

Protein sequence

>MS.gene58945.t1

MASTGDPEYKSSSTPAPASSASGVVDHSKFDLILRFLLFAASVASVVVMVTGNQTEYSRPAKFRYSPAFVYFVAAFSVAGLYSIITTFVSLSAIRKPNLKTKLLLHLIFWDAVMLGILASATGTAGSVAYLGLKGNNHTNWHKICHIYDKFCRHMGASVAVGLFGSIVTLLLIWISAHSIHSRVPK