AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052275

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052275.t1 MTR_4g075340 97.674 258 4 1 1 258 1 256 0.0 501
MS.gene052275.t1 MTR_3g117740 74.900 251 58 3 1 248 1 249 1.44e-125 357
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052275.t1 AT2G16595 56.466 232 93 3 19 248 17 242 9.12e-89 263
MS.gene052275.t1 AT2G21160 60.581 241 87 2 23 258 20 257 1.32e-84 253
MS.gene052275.t1 AT2G21160 59.412 170 61 2 23 187 20 186 1.02e-64 200

Find 59 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGCTTCCTTCAACTCCTTC+TGG 0.233654 4.2:+33436136 MS.gene052275:CDS
AGTTGCCCTCCTTGTCCTCC+TGG 0.292164 4.2:+33438496 MS.gene052275:CDS
TCTGTCTTTCTTGTTACTCT+TGG 0.333410 4.2:+33438473 MS.gene052275:CDS
TGCAGTCAGTAAATTCTTGC+AGG 0.339537 4.2:+33438213 MS.gene052275:CDS
CTCCTGGGGCTATGGATTCA+TGG 0.339701 4.2:+33438512 MS.gene052275:CDS
CTAGTTCCAACTTCAACTTT+AGG 0.354589 4.2:-33439252 None:intergenic
CTTGAATGTTGTTGCAATCA+AGG 0.356465 4.2:+33437483 MS.gene052275:CDS
CCCAAGACTTTGATAGCATT+GGG 0.358768 4.2:+33437041 MS.gene052275:CDS
ACACTTATTGTATCAATTCC+TGG 0.359626 4.2:-33437079 None:intergenic
ATCGCTTCACCGTTCATTCA+AGG 0.374920 4.2:+33436183 MS.gene052275:CDS
TACCAAAGCACCTTCTTTAA+CGG 0.376523 4.2:+33438407 MS.gene052275:CDS
GTTGCCCTCCTTGTCCTCCT+GGG 0.379518 4.2:+33438497 MS.gene052275:CDS
TCCTTGTCCTCCTGGGGCTA+TGG 0.384359 4.2:+33438504 MS.gene052275:CDS
TAGATATTACTACCTTAGAT+AGG 0.392887 4.2:-33438547 None:intergenic
ACTGAAGAAGGAAGTGATAT+TGG 0.393457 4.2:+33437001 MS.gene052275:CDS
TCAAATACAAAACCTATCTA+AGG 0.403503 4.2:+33438535 MS.gene052275:CDS
TTACTGACTGCAAATATGTA+TGG 0.407567 4.2:-33438202 None:intergenic
CCCCAATGCTATCAAAGTCT+TGG 0.420198 4.2:-33437042 None:intergenic
GCCCAAGACTTTGATAGCAT+TGG 0.431971 4.2:+33437040 MS.gene052275:CDS
TGTTCAAAATCTTACTGCTC+AGG 0.451766 4.2:+33437540 MS.gene052275:CDS
TGCCGTTAAAGAAGGTGCTT+TGG 0.453623 4.2:-33438409 None:intergenic
ATTGTATCAATTCCTGGAGC+AGG 0.479754 4.2:-33437073 None:intergenic
GAAAATCCAGAAGGAGTTGA+AGG 0.482193 4.2:-33436142 None:intergenic
ACAAGCAGACGATGATCAAC+AGG 0.483999 4.2:-33437520 None:intergenic
CCCAATGCTATCAAAGTCTT+GGG 0.484284 4.2:-33437041 None:intergenic
TTAAAGAAGGTGCTTTGGTA+TGG 0.496640 4.2:-33438404 None:intergenic
TGAATACAATGCAGTGGTTA+CGG 0.500104 4.2:+33437210 MS.gene052275:intron
AAAGTAGCTTGCGCTGAGGC+TGG 0.500206 4.2:-33438178 None:intergenic
TACAATGCAGTGGTTACGGC+TGG 0.500546 4.2:+33437214 MS.gene052275:intron
GGAAGTGATATTGGCGTCGT+TGG 0.517650 4.2:+33437010 MS.gene052275:CDS
TTCAACAGTGCCGTTAAAGA+AGG 0.525152 4.2:-33438417 None:intergenic
AGGGCTCCTAAAGTTGAAGT+TGG 0.526588 4.2:+33439246 MS.gene052275:CDS
ACTGTTGAAGTTGTTGAAGC+TGG 0.536900 4.2:+33438431 MS.gene052275:CDS
TCCATAGCCCCAGGAGGACA+AGG 0.542721 4.2:-33438505 None:intergenic
TTCAACAGACGAATGGCTAC+AGG 0.544409 4.2:+33439287 MS.gene052275:CDS
TACTGACTGCAAATATGTAT+GGG 0.546293 4.2:-33438201 None:intergenic
GCTAGGTGCCAGTCAGATGA+AGG 0.552570 4.2:+33436965 MS.gene052275:CDS
GATTGCAACAACATTCAAGC+TGG 0.561649 4.2:-33437479 None:intergenic
GACCATGAATCCATAGCCCC+AGG 0.564710 4.2:-33438514 None:intergenic
TTGCCCTCCTTGTCCTCCTG+GGG 0.570226 4.2:+33438498 MS.gene052275:CDS
GTGGATACTACTACTGAAGA+AGG 0.575203 4.2:+33436989 MS.gene052275:CDS
TGATATTGGCGTCGTTGGTG+AGG 0.582105 4.2:+33437015 MS.gene052275:CDS
GAAGACACAGAGCTGCTTGT+GGG 0.585122 4.2:+33437238 MS.gene052275:CDS
TATCCACACCTTCATCTGAC+TGG 0.588486 4.2:-33436973 None:intergenic
AGAAGCAAACCTTGAATGAA+CGG 0.599882 4.2:-33436192 None:intergenic
GAAGGAGTTGAAGGAAGCCA+TGG 0.615324 4.2:-33436133 None:intergenic
CCGACAAGATCAAAACTCCC+AGG 0.627060 4.2:-33438353 None:intergenic
CAGATGCTTCAACAGACGAA+TGG 0.628599 4.2:+33439280 MS.gene052275:CDS
AGAAGACACAGAGCTGCTTG+TGG 0.639497 4.2:+33437237 MS.gene052275:CDS
TAGCCCCAGGAGGACAAGGA+GGG 0.644555 4.2:-33438501 None:intergenic
GTTGAAGTTGTTGAAGCTGG+TGG 0.646915 4.2:+33438434 MS.gene052275:CDS
GTGCCAGTCAGATGAAGGTG+TGG 0.647403 4.2:+33436970 MS.gene052275:CDS
GAATGAACGGTGAAGCGATG+AGG 0.649425 4.2:-33436179 None:intergenic
AGCGAGAGAGAAAATCCAGA+AGG 0.652741 4.2:-33436151 None:intergenic
CCAAGACTTTGATAGCATTG+GGG 0.668986 4.2:+33437042 MS.gene052275:CDS
ATAGCCCCAGGAGGACAAGG+AGG 0.677509 4.2:-33438502 None:intergenic
GCAGACGATGATCAACAGGA+AGG 0.726473 4.2:-33437516 None:intergenic
CATGAATCCATAGCCCCAGG+AGG 0.732654 4.2:-33438511 None:intergenic
TGGGAAAGTAGCTTGCGCTG+AGG 0.756303 4.2:-33438182 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAATTGGTTTTTTATTTT+GGG + chr4.2:33436366-33436385 MS.gene052275:intron 10.0%
!!! TAAAAATTGGTTTTTTATTT+TGG + chr4.2:33436365-33436384 MS.gene052275:intron 10.0%
!!! TATTTTATTTTTTGATCAAA+GGG + chr4.2:33436516-33436535 MS.gene052275:intron 10.0%
!!! TTATTTTATTTTTTGATCAA+AGG + chr4.2:33436515-33436534 MS.gene052275:intron 10.0%
!! AAAAATAAAAAAGAAGCAAT+TGG - chr4.2:33436298-33436317 None:intergenic 15.0%
!! AAAATAAAAAAGAAGCAATT+GGG - chr4.2:33436297-33436316 None:intergenic 15.0%
!! AAAATAGAAAATATTTCACA+AGG - chr4.2:33438720-33438739 None:intergenic 15.0%
!! AAAGAATGTAAAAAAAGAAA+AGG - chr4.2:33437373-33437392 None:intergenic 15.0%
!! GTATTCAATTTACAAAATTA+AGG + chr4.2:33438685-33438704 MS.gene052275:intron 15.0%
!! TATATTATCTCTATACTTAT+TGG + chr4.2:33439328-33439347 MS.gene052275:intron 15.0%
!!! AAAAAATTTTGCAAAATGTA+AGG + chr4.2:33437108-33437127 MS.gene052275:intron 15.0%
!!! AAAATTGGTTTTTTATTTTG+GGG + chr4.2:33436367-33436386 MS.gene052275:intron 15.0%
!!! AAAGATCACATTTTTAATAT+TGG + chr4.2:33436485-33436504 MS.gene052275:intron 15.0%
!!! AAGATCACATTTTTAATATT+GGG + chr4.2:33436486-33436505 MS.gene052275:intron 15.0%
!!! CTTTTAATATAGATTTCAAA+TGG + chr4.2:33436651-33436670 MS.gene052275:intron 15.0%
!!! TATTTTGATCTATTTTTGAA+AGG + chr4.2:33436596-33436615 MS.gene052275:intron 15.0%
!!! TTACATTTTGCAAAATTTTT+TGG - chr4.2:33437109-33437128 None:intergenic 15.0%
!!! TTCTTTTTTTACATTCTTTT+TGG + chr4.2:33437374-33437393 MS.gene052275:intron 15.0%
!! ATGAACATCTAAATTAAACT+TGG + chr4.2:33437576-33437595 MS.gene052275:intron 20.0%
!! TCAAATAATAGCAAATTATC+TGG - chr4.2:33438585-33438604 None:intergenic 20.0%
!!! ATTTTATCAGAAAACAAAGA+GGG + chr4.2:33439227-33439246 MS.gene052275:intron 20.0%
!!! TAAATCTGTATCTTTTTAGA+TGG + chr4.2:33436812-33436831 MS.gene052275:intron 20.0%
!!! TATTTTATCAGAAAACAAAG+AGG + chr4.2:33439226-33439245 MS.gene052275:intron 20.0%
!!! TTTGCTAATTTTTCTCATAA+TGG + chr4.2:33438623-33438642 MS.gene052275:intron 20.0%
!!! TTTTTTTTTCACAGTAAACA+GGG + chr4.2:33438954-33438973 MS.gene052275:intron 20.0%
!!! TTTTTTTTTTCACAGTAAAC+AGG + chr4.2:33438953-33438972 MS.gene052275:intron 20.0%
! AAATATTTCACAAGGTAAAC+AGG - chr4.2:33438712-33438731 None:intergenic 25.0%
! AATATTTCACAAGGTAAACA+GGG - chr4.2:33438711-33438730 None:intergenic 25.0%
! AATGGAATTCCTAATTTAAG+TGG + chr4.2:33436669-33436688 MS.gene052275:intron 25.0%
! ATATATCCTAGCAAAATCTT+TGG - chr4.2:33437975-33437994 None:intergenic 25.0%
! ATATGAAAAACTGAACAACA+TGG - chr4.2:33439357-33439376 None:intergenic 25.0%
! ATTCAATCATATTGGATACT+TGG + chr4.2:33437278-33437297 MS.gene052275:intron 25.0%
! CAAATGGATCATAGATAAAA+CGG - chr4.2:33436237-33436256 None:intergenic 25.0%
! CAGATAGACTACAAATAAAA+GGG - chr4.2:33437424-33437443 None:intergenic 25.0%
! TAACGGTAATTCAATCATAT+TGG + chr4.2:33437270-33437289 MS.gene052275:intron 25.0%
! TAACTTCAAACTAGTAATGT+AGG - chr4.2:33436455-33436474 None:intergenic 25.0%
! TAGATATTACTACCTTAGAT+AGG - chr4.2:33438550-33438569 None:intergenic 25.0%
! TATTTGCAACCACTTAAATT+AGG - chr4.2:33436681-33436700 None:intergenic 25.0%
! TCAAATACAAAACCTATCTA+AGG + chr4.2:33438535-33438554 MS.gene052275:CDS 25.0%
! TCAGATAGACTACAAATAAA+AGG - chr4.2:33437425-33437444 None:intergenic 25.0%
! TTAGCAAATTCAAAATGTGA+AGG - chr4.2:33438611-33438630 None:intergenic 25.0%
!! TTATTTCTTGTGCTAATAAC+AGG + chr4.2:33437451-33437470 MS.gene052275:intron 25.0%
!!! GAACAAATATGTGCATTTTT+TGG + chr4.2:33438806-33438825 MS.gene052275:intron 25.0%
!!! TTTTCTCATAATGGTTTGTT+TGG + chr4.2:33438632-33438651 MS.gene052275:intron 25.0%
AAATCAAACGTCAGAAGTTT+AGG - chr4.2:33439097-33439116 None:intergenic 30.0%
AAATTCCCGTTTCGTATAAA+TGG - chr4.2:33437710-33437729 None:intergenic 30.0%
AATCAAACGTCAGAAGTTTA+GGG - chr4.2:33439096-33439115 None:intergenic 30.0%
ACACTTATTGTATCAATTCC+TGG - chr4.2:33437082-33437101 None:intergenic 30.0%
CATTTCTTGTGAAGGAAATA+CGG + chr4.2:33438041-33438060 MS.gene052275:intron 30.0%
CTTGTGGGTGTAAAAAATAA+CGG + chr4.2:33437253-33437272 MS.gene052275:CDS 30.0%
GAAGATTAAGATTGACTACA+AGG - chr4.2:33437666-33437685 None:intergenic 30.0%
GAGATCAATGAGTATATGTA+TGG - chr4.2:33438303-33438322 None:intergenic 30.0%
GATGAAGGAAATATCAAAGA+CGG - chr4.2:33439175-33439194 None:intergenic 30.0%
TAAAAAAGAAGCAATTGGGT+CGG - chr4.2:33436293-33436312 None:intergenic 30.0%
TAAACTTCTGACGTTTGATT+TGG + chr4.2:33439096-33439115 MS.gene052275:intron 30.0%
TGAAAGCCATTTATACGAAA+CGG + chr4.2:33437701-33437720 MS.gene052275:intron 30.0%
TTTGCTAGGATATATGATGT+CGG + chr4.2:33437980-33437999 MS.gene052275:intron 30.0%
TTTGTATGAATACAATGCAG+TGG + chr4.2:33437204-33437223 MS.gene052275:intron 30.0%
! AAACGTTTTCCAATACCAAA+AGG + chr4.2:33439070-33439089 MS.gene052275:intron 30.0%
! TACTGACTGCAAATATGTAT+GGG - chr4.2:33438204-33438223 None:intergenic 30.0%
! TATTGTGTACTGTCATTTAC+TGG - chr4.2:33438102-33438121 None:intergenic 30.0%
! TGATTTTGCATTTCTTGTGA+AGG + chr4.2:33438033-33438052 MS.gene052275:intron 30.0%
! TGCAATCTAATGCTCATTTT+TGG - chr4.2:33437923-33437942 None:intergenic 30.0%
! TTACTGACTGCAAATATGTA+TGG - chr4.2:33438205-33438224 None:intergenic 30.0%
! TTTTTATGCTATGTGCATGA+TGG + chr4.2:33436910-33436929 MS.gene052275:intron 30.0%
!! TTTTCTTTAAAAGCACAAGC+TGG - chr4.2:33439136-33439155 None:intergenic 30.0%
!!! CTTTTTTTCTTTCTGATTGC+AGG + chr4.2:33438135-33438154 MS.gene052275:intron 30.0%
!!! TGCTTTTAGTTTCTGAACAA+GGG + chr4.2:33438260-33438279 MS.gene052275:intron 30.0%
ACATATAGAACTCCTTCAGA+TGG - chr4.2:33438006-33438025 None:intergenic 35.0%
ACTGAAGAAGGAAGTGATAT+TGG + chr4.2:33437001-33437020 MS.gene052275:CDS 35.0%
ACTGAGGTAGAGTTATTTGA+AGG + chr4.2:33436700-33436719 MS.gene052275:intron 35.0%
AGAAGATGAAATGCTAGCTA+GGG - chr4.2:33438851-33438870 None:intergenic 35.0%
AGAAGCAAACCTTGAATGAA+CGG - chr4.2:33436195-33436214 None:intergenic 35.0%
CTAGTTCCAACTTCAACTTT+AGG - chr4.2:33439255-33439274 None:intergenic 35.0%
CTTGAATGTTGTTGCAATCA+AGG + chr4.2:33437483-33437502 MS.gene052275:CDS 35.0%
GAAAGCCATTTATACGAAAC+GGG + chr4.2:33437702-33437721 MS.gene052275:intron 35.0%
GATATTTCGTTTCAGATGTC+TGG - chr4.2:33439398-33439417 None:intergenic 35.0%
GCAAATTCAAAATGTGAAGG+CGG - chr4.2:33438608-33438627 None:intergenic 35.0%
GCATTGTTTAATCTTGTGCA+TGG + chr4.2:33436538-33436557 MS.gene052275:intron 35.0%
GTATATTGTGTTCCCTTGAA+AGG + chr4.2:33437769-33437788 MS.gene052275:intron 35.0%
TCTGTCTTTCTTGTTACTCT+TGG + chr4.2:33438473-33438492 MS.gene052275:CDS 35.0%
TGAATACAATGCAGTGGTTA+CGG + chr4.2:33437210-33437229 MS.gene052275:intron 35.0%
TGTTCAAAATCTTACTGCTC+AGG + chr4.2:33437540-33437559 MS.gene052275:CDS 35.0%
TTAAGTGGTTGCAAATACTG+AGG + chr4.2:33436684-33436703 MS.gene052275:intron 35.0%
TTTGCATGCATTGTGATCTT+TGG + chr4.2:33436316-33436335 MS.gene052275:intron 35.0%
! AAGTAGCCAAAGATTTTGCT+AGG + chr4.2:33437966-33437985 MS.gene052275:intron 35.0%
! AGATGTCTGGTTTATGCTAT+AGG - chr4.2:33439385-33439404 None:intergenic 35.0%
! AGTAATTTGCAAAATCCGAC+AGG - chr4.2:33436879-33436898 None:intergenic 35.0%
! GAGGATTTGAAATTACTCCA+AGG - chr4.2:33437797-33437816 None:intergenic 35.0%
! GGTTTGTTTGGTTTATGAAG+AGG + chr4.2:33438644-33438663 MS.gene052275:intron 35.0%
! GTAATTTGCAAAATCCGACA+GGG - chr4.2:33436878-33436897 None:intergenic 35.0%
! TACCAAAGCACCTTCTTTAA+CGG + chr4.2:33438407-33438426 MS.gene052275:CDS 35.0%
! TGATTGCAGGTTTTCAACAA+TGG + chr4.2:33438148-33438167 MS.gene052275:intron 35.0%
!! TGCATGATGGTTTTTGATTG+TGG + chr4.2:33436923-33436942 MS.gene052275:intron 35.0%
!! TTAAAGAAGGTGCTTTGGTA+TGG - chr4.2:33438407-33438426 None:intergenic 35.0%
!! TTTTCCATTTGCTAGTTGCT+AGG + chr4.2:33436948-33436967 MS.gene052275:intron 35.0%
!!! CTGCTTTTAGTTTCTGAACA+AGG + chr4.2:33438259-33438278 MS.gene052275:intron 35.0%
!!! GGGGTATTTTGATCTTTGTT+GGG + chr4.2:33436386-33436405 MS.gene052275:intron 35.0%
!!! TAATGCTCATTTTTGGCACA+GGG - chr4.2:33437916-33437935 None:intergenic 35.0%
!!! TGGGGTATTTTGATCTTTGT+TGG + chr4.2:33436385-33436404 MS.gene052275:intron 35.0%
!!! TGTTATTTTACTTGCCCTGT+CGG + chr4.2:33436861-33436880 MS.gene052275:intron 35.0%
AATGTAAGGATCGTTCTGTC+TGG + chr4.2:33437122-33437141 MS.gene052275:intron 40.0%
ACTAAAAGCAGTTGCCTTCA+AGG - chr4.2:33438250-33438269 None:intergenic 40.0%
ACTGTTGAAGTTGTTGAAGC+TGG + chr4.2:33438431-33438450 MS.gene052275:CDS 40.0%
AGCAAACACGAGAATATCAG+AGG - chr4.2:33439198-33439217 None:intergenic 40.0%
ATTGTATCAATTCCTGGAGC+AGG - chr4.2:33437076-33437095 None:intergenic 40.0%
CAAACTAGTAATGTAGGTGC+AGG - chr4.2:33436449-33436468 None:intergenic 40.0%
CATGCAGAAAACGAAGCAAA+TGG - chr4.2:33436253-33436272 None:intergenic 40.0%
CCCAATGCTATCAAAGTCTT+GGG - chr4.2:33437044-33437063 None:intergenic 40.0%
CTTACTGCTCAGGTAGTAAT+TGG + chr4.2:33437550-33437569 MS.gene052275:intron 40.0%
GAAAATCCAGAAGGAGTTGA+AGG - chr4.2:33436145-33436164 None:intergenic 40.0%
GAAGGAAATATCAAAGACGG+AGG - chr4.2:33439172-33439191 None:intergenic 40.0%
GAGAAGATGAAATGCTAGCT+AGG - chr4.2:33438852-33438871 None:intergenic 40.0%
GATCTACACGTGACAAAGAT+TGG + chr4.2:33437822-33437841 MS.gene052275:intron 40.0%
GATTGCAACAACATTCAAGC+TGG - chr4.2:33437482-33437501 None:intergenic 40.0%
GCAAACACGAGAATATCAGA+GGG - chr4.2:33439197-33439216 None:intergenic 40.0%
GTCATTTACTGGATCAGAAC+AGG - chr4.2:33438091-33438110 None:intergenic 40.0%
GTGGATACTACTACTGAAGA+AGG + chr4.2:33436989-33437008 MS.gene052275:CDS 40.0%
TAAAAGCCATCTCCTACAGA+TGG - chr4.2:33437856-33437875 None:intergenic 40.0%
TCATCTTCTCTCGATCACAA+TGG + chr4.2:33438862-33438881 MS.gene052275:intron 40.0%
TGCAGTCAGTAAATTCTTGC+AGG + chr4.2:33438213-33438232 MS.gene052275:CDS 40.0%
TGGAAGCTTGATTCAGCATT+TGG + chr4.2:33436832-33436851 MS.gene052275:intron 40.0%
TTACTATCCAACCTCTTTCC+AGG - chr4.2:33437741-33437760 None:intergenic 40.0%
TTGCCTTGTATTGTCATCCA+GGG + chr4.2:33439423-33439442 MS.gene052275:intron 40.0%
TTTCAAATCCTCCTGGAAAG+AGG + chr4.2:33437727-33437746 MS.gene052275:intron 40.0%
TTTGCCTTGTATTGTCATCC+AGG + chr4.2:33439422-33439441 MS.gene052275:intron 40.0%
TTTGTCACGTGTAGATCAAC+AGG - chr4.2:33437819-33437838 None:intergenic 40.0%
! AGGTATTGCCATTACCTTGA+AGG + chr4.2:33438233-33438252 MS.gene052275:intron 40.0%
! CCAAGACTTTGATAGCATTG+GGG + chr4.2:33437042-33437061 MS.gene052275:CDS 40.0%
! CCCAAGACTTTGATAGCATT+GGG + chr4.2:33437041-33437060 MS.gene052275:CDS 40.0%
! TACTCCAAGGTACCTTTCAA+GGG - chr4.2:33437784-33437803 None:intergenic 40.0%
! TTACTCCAAGGTACCTTTCA+AGG - chr4.2:33437785-33437804 None:intergenic 40.0%
! TTCAACAGTGCCGTTAAAGA+AGG - chr4.2:33438420-33438439 None:intergenic 40.0%
!! AGTTTAGGGCCTTTTGGTAT+TGG - chr4.2:33439082-33439101 None:intergenic 40.0%
!!! CTAATGCTCATTTTTGGCAC+AGG - chr4.2:33437917-33437936 None:intergenic 40.0%
AAATCCTCCTGGAAAGAGGT+TGG + chr4.2:33437731-33437750 MS.gene052275:intron 45.0%
ACAAGCAGACGATGATCAAC+AGG - chr4.2:33437523-33437542 None:intergenic 45.0%
ACGGGAATTTCAAATCCTCC+TGG + chr4.2:33437720-33437739 MS.gene052275:intron 45.0%
ACTGAGTATATGCAGTTCCC+TGG - chr4.2:33439443-33439462 None:intergenic 45.0%
AGCAGTTGCCTTCAAGGTAA+TGG - chr4.2:33438244-33438263 None:intergenic 45.0%
AGCGAGAGAGAAAATCCAGA+AGG - chr4.2:33436154-33436173 None:intergenic 45.0%
AGGGCTCCTAAAGTTGAAGT+TGG + chr4.2:33439246-33439265 MS.gene052275:CDS 45.0%
ATATGATGTCGGCCATCTGA+AGG + chr4.2:33437991-33438010 MS.gene052275:intron 45.0%
ATCGCTTCACCGTTCATTCA+AGG + chr4.2:33436183-33436202 MS.gene052275:CDS 45.0%
CAGATGCTTCAACAGACGAA+TGG + chr4.2:33439280-33439299 MS.gene052275:CDS 45.0%
CCCCAATGCTATCAAAGTCT+TGG - chr4.2:33437045-33437064 None:intergenic 45.0%
CGAAGCCAAGAGTCAAATCA+GGG + chr4.2:33437397-33437416 MS.gene052275:intron 45.0%
CGAGAATATCAGAGGGATGA+AGG - chr4.2:33439190-33439209 None:intergenic 45.0%
GAAGCTTGAAATCACAGTGC+TGG - chr4.2:33437309-33437328 None:intergenic 45.0%
GTTCCCTGGATGACAATACA+AGG - chr4.2:33439429-33439448 None:intergenic 45.0%
GTTGAAGTTGTTGAAGCTGG+TGG + chr4.2:33438434-33438453 MS.gene052275:CDS 45.0%
TACTTGCCATCTGTAGGAGA+TGG + chr4.2:33437847-33437866 MS.gene052275:intron 45.0%
TATCCACACCTTCATCTGAC+TGG - chr4.2:33436976-33436995 None:intergenic 45.0%
TTCAACAGACGAATGGCTAC+AGG + chr4.2:33439287-33439306 MS.gene052275:CDS 45.0%
TTGAAGGAACGACGACAACA+TGG + chr4.2:33436716-33436735 MS.gene052275:intron 45.0%
TTGGTCTACTTGCCATCTGT+AGG + chr4.2:33437841-33437860 MS.gene052275:intron 45.0%
! GCCCAAGACTTTGATAGCAT+TGG + chr4.2:33437040-33437059 MS.gene052275:CDS 45.0%
!! CCTGGGAGTTTTGATCTTGT+CGG + chr4.2:33438353-33438372 MS.gene052275:CDS 45.0%
!! GTCAGAAGTTTAGGGCCTTT+TGG - chr4.2:33439088-33439107 None:intergenic 45.0%
!! TCTGACGTTTGATTTGGTCC+TGG + chr4.2:33439102-33439121 MS.gene052275:intron 45.0%
!! TGCCGTTAAAGAAGGTGCTT+TGG - chr4.2:33438412-33438431 None:intergenic 45.0%
!! TGTTCCCTTGAAAGGTACCT+TGG + chr4.2:33437777-33437796 MS.gene052275:intron 45.0%
!!! TTATAATTTGTATTAAAAAT+TGG + chr4.2:33436352-33436371 MS.gene052275:intron 5.0%
!!! TTTTTTTTTTTTTTTTTTTG+AGG + chr4.2:33436570-33436589 MS.gene052275:intron 5.0%
AGAAGACACAGAGCTGCTTG+TGG + chr4.2:33437237-33437256 MS.gene052275:CDS 50.0%
CACAAGCTGGAGCAATAACC+AGG - chr4.2:33439123-33439142 None:intergenic 50.0%
CAGACGAATGGCTACAGGTA+TGG + chr4.2:33439292-33439311 MS.gene052275:intron 50.0%
CCGACAAGATCAAAACTCCC+AGG - chr4.2:33438356-33438375 None:intergenic 50.0%
CTATCCAACCTCTTTCCAGG+AGG - chr4.2:33437738-33437757 None:intergenic 50.0%
GAAGACACAGAGCTGCTTGT+GGG + chr4.2:33437238-33437257 MS.gene052275:CDS 50.0%
GAATGAACGGTGAAGCGATG+AGG - chr4.2:33436182-33436201 None:intergenic 50.0%
GCAGACGATGATCAACAGGA+AGG - chr4.2:33437519-33437538 None:intergenic 50.0%
GCGAAGCCAAGAGTCAAATC+AGG + chr4.2:33437396-33437415 MS.gene052275:intron 50.0%
GGAAGTGATATTGGCGTCGT+TGG + chr4.2:33437010-33437029 MS.gene052275:CDS 50.0%
GGCACCTAGCAACTAGCAAA+TGG - chr4.2:33436955-33436974 None:intergenic 50.0%
GTCACGTGTAGATCAACAGG+AGG - chr4.2:33437816-33437835 None:intergenic 50.0%
TACAATGCAGTGGTTACGGC+TGG + chr4.2:33437214-33437233 MS.gene052275:intron 50.0%
TGATATTGGCGTCGTTGGTG+AGG + chr4.2:33437015-33437034 MS.gene052275:CDS 50.0%
TGGCTTCCTTCAACTCCTTC+TGG + chr4.2:33436136-33436155 MS.gene052275:CDS 50.0%
TTTGGCACAGGGCAGTAACT+GGG - chr4.2:33437905-33437924 None:intergenic 50.0%
! AGGGACCCTGATTTGACTCT+TGG - chr4.2:33437405-33437424 None:intergenic 50.0%
! GATTTTTCCGTGTGCAGCCT+GGG + chr4.2:33438336-33438355 MS.gene052275:intron 50.0%
! TGATTTTTCCGTGTGCAGCC+TGG + chr4.2:33438335-33438354 MS.gene052275:intron 50.0%
! TTTTGGCACAGGGCAGTAAC+TGG - chr4.2:33437906-33437925 None:intergenic 50.0%
CAAAACTCCCAGGCTGCACA+CGG - chr4.2:33438346-33438365 None:intergenic 55.0%
CATGAATCCATAGCCCCAGG+AGG - chr4.2:33438514-33438533 None:intergenic 55.0%
CTCCTGGGGCTATGGATTCA+TGG + chr4.2:33438512-33438531 MS.gene052275:CDS 55.0%
GACCATGAATCCATAGCCCC+AGG - chr4.2:33438517-33438536 None:intergenic 55.0%
GCTAGGTGCCAGTCAGATGA+AGG + chr4.2:33436965-33436984 MS.gene052275:CDS 55.0%
GTGCCAGTCAGATGAAGGTG+TGG + chr4.2:33436970-33436989 MS.gene052275:CDS 55.0%
! AAAGTAGCTTGCGCTGAGGC+TGG - chr4.2:33438181-33438200 None:intergenic 55.0%
! TGGGAAAGTAGCTTGCGCTG+AGG - chr4.2:33438185-33438204 None:intergenic 55.0%
AGTTGCCCTCCTTGTCCTCC+TGG + chr4.2:33438496-33438515 MS.gene052275:CDS 60.0%
ATAGCCCCAGGAGGACAAGG+AGG - chr4.2:33438505-33438524 None:intergenic 60.0%
GTTGCCCTCCTTGTCCTCCT+GGG + chr4.2:33438497-33438516 MS.gene052275:CDS 60.0%
TAGCCCCAGGAGGACAAGGA+GGG - chr4.2:33438504-33438523 None:intergenic 60.0%
TCCATAGCCCCAGGAGGACA+AGG - chr4.2:33438508-33438527 None:intergenic 60.0%
TCCTTGTCCTCCTGGGGCTA+TGG + chr4.2:33438504-33438523 MS.gene052275:CDS 60.0%
TTGCCCTCCTTGTCCTCCTG+GGG + chr4.2:33438498-33438517 MS.gene052275:CDS 60.0%
! GGGGACTTTTCTCCTGCTCC+AGG + chr4.2:33437061-33437080 MS.gene052275:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 33436135 33439494 33436135 ID=MS.gene052275
chr4.2 mRNA 33436135 33439494 33436135 ID=MS.gene052275.t1;Parent=MS.gene052275
chr4.2 exon 33436135 33436204 33436135 ID=MS.gene052275.t1.exon1;Parent=MS.gene052275.t1
chr4.2 CDS 33436135 33436204 33436135 ID=cds.MS.gene052275.t1;Parent=MS.gene052275.t1
chr4.2 exon 33436963 33437124 33436963 ID=MS.gene052275.t1.exon2;Parent=MS.gene052275.t1
chr4.2 CDS 33436963 33437124 33436963 ID=cds.MS.gene052275.t1;Parent=MS.gene052275.t1
chr4.2 exon 33437224 33437274 33437224 ID=MS.gene052275.t1.exon3;Parent=MS.gene052275.t1
chr4.2 CDS 33437224 33437274 33437224 ID=cds.MS.gene052275.t1;Parent=MS.gene052275.t1
chr4.2 exon 33437473 33437561 33437473 ID=MS.gene052275.t1.exon4;Parent=MS.gene052275.t1
chr4.2 CDS 33437473 33437561 33437473 ID=cds.MS.gene052275.t1;Parent=MS.gene052275.t1
chr4.2 exon 33438157 33438234 33438157 ID=MS.gene052275.t1.exon5;Parent=MS.gene052275.t1
chr4.2 CDS 33438157 33438234 33438157 ID=cds.MS.gene052275.t1;Parent=MS.gene052275.t1
chr4.2 exon 33438353 33438556 33438353 ID=MS.gene052275.t1.exon6;Parent=MS.gene052275.t1
chr4.2 CDS 33438353 33438556 33438353 ID=cds.MS.gene052275.t1;Parent=MS.gene052275.t1
chr4.2 exon 33439237 33439308 33439237 ID=MS.gene052275.t1.exon7;Parent=MS.gene052275.t1
chr4.2 CDS 33439237 33439308 33439237 ID=cds.MS.gene052275.t1;Parent=MS.gene052275.t1
chr4.2 exon 33439444 33439494 33439444 ID=MS.gene052275.t1.exon8;Parent=MS.gene052275.t1
chr4.2 CDS 33439444 33439494 33439444 ID=cds.MS.gene052275.t1;Parent=MS.gene052275.t1
Gene Sequence

>MS.gene052275

ATGGCTTCCTTCAACTCCTTCTGGATTTTCTCTCTCGCTCTTCTCCTCATCGCTTCACCGTTCATTCAAGGTTTGCTTCTCTACATTATAGATCTAGATCCGTTTTATCTATGATCCATTTGCTTCGTTTTCTGCATGTTACGATCACTTTTGTGCCGACCCAATTGCTTCTTTTTTATTTTTTGCATGCATTGTGATCTTTGGTCTGATTTATAATTTATAATTTGTATTAAAAATTGGTTTTTTATTTTGGGGTATTTTGATCTTTGTTGGGAATATATGTGTTTTGTTATGATTTTATGATTTCATTGCCTGCACCTACATTACTAGTTTGAAGTTATATAGAGTGAAAAGATCACATTTTTAATATTGGGTATTGTTTATTTTATTTTTTGATCAAAGGGCATTGTTTAATCTTGTGCATGGCACTTTAAGTTTTTTTTTTTTTTTTTTTGAGGTTGTATTTTGATCTATTTTTGAAAGGATTCTTTGTATTTAAGTAGATTCAATGATGAACTTTTAATATAGATTTCAAATGGAATTCCTAATTTAAGTGGTTGCAAATACTGAGGTAGAGTTATTTGAAGGAACGACGACAACATGGTGCAATTGAATGAATTCTGTATTAGTCTAGTTCTGTTAATTTAATTTAATCTAAATTTCACTGTTCAGTAATCTAAATCTGTATCTTTTTAGATGGAAGCTTGATTCAGCATTTGGTTTATGTGTTATTTTACTTGCCCTGTCGGATTTTGCAAATTACTTGATAAGAATGTTTTTATGCTATGTGCATGATGGTTTTTGATTGTGGTTTTTTCCATTTGCTAGTTGCTAGGTGCCAGTCAGATGAAGGTGTGGATACTACTACTGAAGAAGGAAGTGATATTGGCGTCGTTGGTGAGGAAGCCCAAGACTTTGATAGCATTGGGGACTTTTCTCCTGCTCCAGGAATTGATACAATAAGTGTATTTCCAAAAAATTTTGCAAAATGTAAGGATCGTTCTGTCTGGTACAAATAATTTAGCTTTAGTTTTGTTTTCTGTATTTGATAATGTGTTGATTTTGTCTGTTTGTATGAATACAATGCAGTGGTTACGGCTGGAGAAGACACAGAGCTGCTTGTGGGTGTAAAAAATAACGGTAATTCAATCATATTGGATACTTGGTGTATCCAGCACTGTGATTTCAAGCTTCTTAAATGTTTTGTTCTCGTTGTTTAGTATTTATATTTTATGCCTTTTCTTTTTTTACATTCTTTTTGGCGAAGCCAAGAGTCAAATCAGGGTCCCTTTTATTTGTAGTCTATCTGACTTCGTTTATTTCTTGTGCTAATAACAGGAGACTCCAGCTTGAATGTTGTTGCAATCAAGGCTAGTGTTCACCTTCCTGTTGATCATCGTCTGCTTGTTCAAAATCTTACTGCTCAGGTAGTAATTGGTTAATGAACATCTAAATTAAACTTGGCTATTTATTTGTCAAAAATTGTACTGCTCATGTTAGTTTAAATGATTATTATTTATGCTATCTTCCTTGTAGTCAATCTTAATCTTCTAATGCTCAAATTGATGAAAGCCATTTATACGAAACGGGAATTTCAAATCCTCCTGGAAAGAGGTTGGATAGTAATTAGTAAAGTATATTGTGTTCCCTTGAAAGGTACCTTGGAGTAATTTCAAATCCTCCTGTTGATCTACACGTGACAAAGATTGGTCTACTTGCCATCTGTAGGAGATGGCTTTTAATCGTGTATGAATGTCATAGTGCTGACCCAGTTACTGCCCTGTGCCAAAAATGAGCATTAGATTGCACTTGTGATATTTTTGATACATGCAAGTAGCCAAAGATTTTGCTAGGATATATGATGTCGGCCATCTGAAGGAGTTCTATATGTTTGTACATGATTTTGCATTTCTTGTGAAGGAAATACGGTTCTGCAATGTTGTGTCAGTATCACCTGTTCTGATCCAGTAAATGACAGTACACAATAAATATAAAGCAAACTTTTTTTCTTTCTGATTGCAGGTTTTCAACAATGGTTCTGTACCAGCCTCAGCGCAAGCTACTTTCCCATACATATTTGCAGTCAGTAAATTCTTGCAGGTATTGCCATTACCTTGAAGGCAACTGCTTTTAGTTTCTGAACAAGGGCAAGATAGACAGCACTTCCATACATATACTCATTGATCTCATAACAACTTAATGATTTTTCCGTGTGCAGCCTGGGAGTTTTGATCTTGTCGGCACTATTATTTATGAGATAGACGAGCAACCATACCAAAGCACCTTCTTTAACGGCACTGTTGAAGTTGTTGAAGCTGGTGGTATTCTTAGCATCGAATCTGTCTTTCTTGTTACTCTTGGAGTTGCCCTCCTTGTCCTCCTGGGGCTATGGATTCATGGTCAAATACAAAACCTATCTAAGGTAGTAATATCTATATTTTTTTTTTCCAGATAATTTGCTATTATTTGACCGCCTTCACATTTTGAATTTGCTAATTTTTCTCATAATGGTTTGTTTGGTTTATGAAGAGGTTTTTGTTTTCATTTCTTGTATTCAATTTACAAAATTAAGGCCCTGTTTACCTTGTGAAATATTTTCTATTTTCATTTTCTAAAATATGTGTTAATTTTACTTGCTAGCAAAAACATAATATTGATAGACTCTTGAAGTGAACAAATATGTGCATTTTTTGGAAACAACGAAATGCATCATCCCTAGCTAGCATTTCATCTTCTCTCGATCACAATGGTTCACTCTAAGAGTCTCACATATTCTTATTAGCAAGTTAAATGAGCACATATTTTAGAAAATTAATTTTTTTTTTTTTCACAGTAAACAGGGCTTAAACTGCACACTGTACAGCAGTTGTGTTCAATGCTTTCAGTACAAACATTTGAAAACAAAAAATAGAGTGAAAAATAAAATGAAAATAACAAACGTTTTCCAATACCAAAAGGCCCTAAACTTCTGACGTTTGATTTGGTCCTGGTTATTGCTCCAGCTTGTGCTTTTAAAGAAAATCTTCAAAGAAAACCTCCGTCTTTGATATTTCCTTCATCCCTCTGATATTCTCGTGTTTGCTTATATGAATATTTTATCAGAAAACAAAGAGGGCTCCTAAAGTTGAAGTTGGAACTAGATCTACAGATGCTTCAACAGACGAATGGCTACAGGTATGGTCTTATGTTGCTGTATATTATCTCTATACTTATTGGATGCCATGTTGTTCAGTTTTTCATATTTAAACCTATAGCATAAACCAGACATCTGAAACGAAATATCTTTTTTTGCCTTGTATTGTCATCCAGGGAACTGCATATACTCAGTCTCTGTCGAGCAAATCGAAGAAGAAGAAGTAG
Protein sequence

>MS.gene052275.t1

MASFNSFWIFSLALLLIASPFIQVARCQSDEGVDTTTEEGSDIGVVGEEAQDFDSIGDFSPAPGIDTISVFPKNFAKLVTAGEDTELLVGVKNNGDSSLNVVAIKASVHLPVDHRLLVQNLTAQVFNNGSVPASAQATFPYIFAVSKFLQPGSFDLVGTIIYEIDEQPYQSTFFNGTVEVVEAGGILSIESVFLVTLGVALLVLLGLWIHGQIQNLSKKTKRAPKVEVGTRSTDASTDEWLQGTAYTQSLSSKSKKKK