AlfalfaGEDB | MS.gene052439

M. sativa cultivar XinJiangDaYe / MS.gene052439

KEGG: K03627
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF01381.25 PF08523.13

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052439.t1	AES76734.2	97.3	146	4	0	1	146	4	149	2.60E-70	274.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052439.t1	Q9LV58	71.6	148	40	1	1	146	1	148	8.2e-49	194.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052439.t1	G7KNH9	97.3	146	4	0	1	146	4	149	1.9e-70	274.6

TFs/TRs:

Gene ID	Type	Classification
MS.gene052439.t1	TR	MBF1

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene94585	MS.gene052439	PPI
MS.gene045483	MS.gene052439	PPI
MS.gene052439	MS.gene073154	PPI
MS.gene91241	MS.gene052439	PPI
MS.gene052439	MS.gene73865	PPI
MS.gene052439	MS.gene35285	PPI
MS.gene052439	MS.gene94585	PPI
MS.gene006697	MS.gene052439	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052439.t1	MTR_6g086280	97.260	146	4	0	1	146	4	149	1.78e-100	285
MS.gene052439.t1	MTR_6g086280	97.222	144	4	0	1	144	4	147	4.12e-99	281
MS.gene052439.t1	MTR_2g084220	50.000	138	69	0	6	143	2	139	4.64e-43	139
MS.gene052439.t1	MTR_4g080090	50.725	138	68	0	6	143	4	141	9.39e-42	136
MS.gene052439.t1	MTR_6g018330	42.105	133	61	1	11	143	26	142	1.70e-26	97.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052439.t1	AT3G24500	71.622	148	40	1	1	146	1	148	9.93e-69	204
MS.gene052439.t1	AT3G58680	52.174	138	66	0	6	143	4	141	4.19e-44	142
MS.gene052439.t1	AT2G42680	50.725	138	68	0	6	143	4	141	7.40e-43	139
MS.gene052439.t1	AT3G24500	58.947	95	37	1	1	93	1	95	8.16e-33	112

Find 45 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTTCGGAGGGCTTGGTTTA+TGG	0.142766	6.1:-75129326	None:intergenic
GGGGAAAATGGAGAAGGTTT+TGG	0.237217	6.1:+75129615	MS.gene052439:CDS
TGTTATTAATCAAGGTGTTT+TGG	0.256794	6.1:+75129594	MS.gene052439:CDS
GTTAAACTTAGGGGTAAAAT+TGG	0.277746	6.1:+75129640	MS.gene052439:CDS
CTGCTCCGGTTCGGAGGGCT+TGG	0.299889	6.1:-75129333	None:intergenic
AGGGCTTGGTTTATGGCCTT+TGG	0.306456	6.1:-75129319	None:intergenic
TTACGAAGGTCTTGTGCTTT+GGG	0.313592	6.1:-75129295	None:intergenic
GTTACGAAGGTCTTGTGCTT+TGG	0.324998	6.1:-75129296	None:intergenic
TGAGAGGGTTCAGGTTGTTC+AGG	0.327268	6.1:+75129552	MS.gene052439:CDS
CGCGATACAGAAAGCGCGTT+TGG	0.332684	6.1:+75129489	MS.gene052439:CDS
GAAGATGAGTCAGGGTGATT+TGG	0.359012	6.1:+75129516	MS.gene052439:CDS
AAGGTCTTGTGCTTTGGGTT+TGG	0.379299	6.1:-75129290	None:intergenic
ATTGATTAATGAGAGGGTTC+AGG	0.382828	6.1:+75129543	MS.gene052439:CDS
GGGCTTGGTTTATGGCCTTT+GGG	0.397588	6.1:-75129318	None:intergenic
AATTTCCTCGCGTTTAAAAC+CGG	0.402472	6.1:-75129412	None:intergenic
ATTAGATGAAGCGGCTGAAC+CGG	0.409634	6.1:+75129432	MS.gene052439:CDS
CTGAACCGGCAGCGTTGGAG+CGG	0.424143	6.1:+75129446	MS.gene052439:CDS
GCAGCGTTGGAGCGGGTTGG+TGG	0.463582	6.1:+75129454	MS.gene052439:CDS
AGCGGCTGAACCGGCAGCGT+TGG	0.470397	6.1:+75129441	MS.gene052439:CDS
ATAAACCAAGCCCTCCGAAC+CGG	0.471149	6.1:+75129328	MS.gene052439:CDS
TTTGGATAAGAAGATGAGTC+AGG	0.478923	6.1:+75129507	MS.gene052439:CDS
GGTAAAGCTGTTATTAATCA+AGG	0.485331	6.1:+75129586	MS.gene052439:CDS
TGGGAACAATAAAACAAGAC+TGG	0.494179	6.1:+75129245	MS.gene052439:CDS
GACTGTTAAGACCTCTGCTC+CGG	0.499262	6.1:-75129347	None:intergenic
TTTAAAACCGGTCCGCTCGT+TGG	0.502945	6.1:-75129400	None:intergenic
TTAAGACCTCTGCTCCGGTT+CGG	0.505245	6.1:-75129342	None:intergenic
GACCTCTGCTCCGGTTCGGA+GGG	0.511130	6.1:-75129338	None:intergenic
TGAACCGGCAGCGTTGGAGC+GGG	0.513996	6.1:+75129447	MS.gene052439:CDS
GGCGAAATTGATTAATGAGA+GGG	0.515808	6.1:+75129537	MS.gene052439:CDS
AGACCTCTGCTCCGGTTCGG+AGG	0.519624	6.1:-75129339	None:intergenic
ATTGTTCCCACGGTTCGTGT+CGG	0.534812	6.1:-75129232	None:intergenic
CGCGAGGAAATTAGATGAAG+CGG	0.544491	6.1:+75129423	MS.gene052439:CDS
CCAACCCGCTCCAACGCTGC+CGG	0.561045	6.1:-75129451	None:intergenic
GTTGTTCAGGAGTATGAGAA+TGG	0.565499	6.1:+75129565	MS.gene052439:CDS
TACGATGCCGACACGAACCG+TGG	0.582591	6.1:+75129225	None:intergenic
AGCCCTCCGAACCGGAGCAG+AGG	0.582656	6.1:+75129336	MS.gene052439:CDS
CCGGCAGCGTTGGAGCGGGT+TGG	0.582916	6.1:+75129451	MS.gene052439:CDS
TGGCGAAATTGATTAATGAG+AGG	0.583756	6.1:+75129536	MS.gene052439:CDS
TTATGGCCTTTGGGTTACGA+AGG	0.594530	6.1:-75129309	None:intergenic
GGGAACAATAAAACAAGACT+GGG	0.610956	6.1:+75129246	MS.gene052439:CDS
AAAACAAGACTGGGAACCAG+TGG	0.626445	6.1:+75129255	MS.gene052439:CDS
ACAGTCAAGAAACCAACAGC+CGG	0.641212	6.1:+75129364	MS.gene052439:CDS
TTGGATAAGAAGATGAGTCA+GGG	0.654305	6.1:+75129508	MS.gene052439:CDS
ACGATGCCGACACGAACCGT+GGG	0.663582	6.1:+75129226	None:intergenic
ACAAGACCTTCGTAACCCAA+AGG	0.679598	6.1:+75129303	MS.gene052439:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	GTTATTAATCAAGGTGTTTT+GGG	+	chr6.1:75129595-75129614	MS.gene052439:CDS	25.0%
!!!	TGTTATTAATCAAGGTGTTT+TGG	+	chr6.1:75129594-75129613	MS.gene052439:CDS	25.0%
!!!	TTATTAATCAAGGTGTTTTG+GGG	+	chr6.1:75129596-75129615	MS.gene052439:CDS	25.0%
	GGTAAAGCTGTTATTAATCA+AGG	+	chr6.1:75129586-75129605	MS.gene052439:CDS	30.0%
	GTTAAACTTAGGGGTAAAAT+TGG	+	chr6.1:75129640-75129659	MS.gene052439:CDS	30.0%
	ATTGATTAATGAGAGGGTTC+AGG	+	chr6.1:75129543-75129562	MS.gene052439:CDS	35.0%
	GGCGAAATTGATTAATGAGA+GGG	+	chr6.1:75129537-75129556	MS.gene052439:CDS	35.0%
	GGGAACAATAAAACAAGACT+GGG	+	chr6.1:75129246-75129265	MS.gene052439:CDS	35.0%
	TGGCGAAATTGATTAATGAG+AGG	+	chr6.1:75129536-75129555	MS.gene052439:CDS	35.0%
	TGGGAACAATAAAACAAGAC+TGG	+	chr6.1:75129245-75129264	MS.gene052439:CDS	35.0%
	TTGGATAAGAAGATGAGTCA+GGG	+	chr6.1:75129508-75129527	MS.gene052439:CDS	35.0%
	TTTGGATAAGAAGATGAGTC+AGG	+	chr6.1:75129507-75129526	MS.gene052439:CDS	35.0%
!	AATTTCCTCGCGTTTAAAAC+CGG	-	chr6.1:75129415-75129434	None:intergenic	35.0%
!!!	GTCTTGTTTTATTGTTCCCA+CGG	-	chr6.1:75129245-75129264	None:intergenic	35.0%
	TCGAACAAAAAACCAACGAG+CGG	+	chr6.1:75129388-75129407	MS.gene052439:CDS	40.0%
!	GGAAAATGGAGAAGGTTTTG+GGG	+	chr6.1:75129617-75129636	MS.gene052439:CDS	40.0%
!	GGGAAAATGGAGAAGGTTTT+GGG	+	chr6.1:75129616-75129635	MS.gene052439:CDS	40.0%
!	GTTGTTCAGGAGTATGAGAA+TGG	+	chr6.1:75129565-75129584	MS.gene052439:CDS	40.0%
!	TTACGAAGGTCTTGTGCTTT+GGG	-	chr6.1:75129298-75129317	None:intergenic	40.0%
!!	GAAAATGGAGAAGGTTTTGG+GGG	+	chr6.1:75129618-75129637	MS.gene052439:CDS	40.0%
!!!	AGGTTTTGGGGGTTAAACTT+AGG	+	chr6.1:75129629-75129648	MS.gene052439:CDS	40.0%
!!!	CGTTGGTTTTTTGTTCGAAC+CGG	-	chr6.1:75129386-75129405	None:intergenic	40.0%
!!!	GGTTTTGGGGGTTAAACTTA+GGG	+	chr6.1:75129630-75129649	MS.gene052439:CDS	40.0%
!!!	GTTTTGGGGGTTAAACTTAG+GGG	+	chr6.1:75129631-75129650	MS.gene052439:CDS	40.0%
!!!	TCAAGGTGTTTTGGGGAAAA+TGG	+	chr6.1:75129603-75129622	MS.gene052439:CDS	40.0%
!!!	TGTTTTGGGGAAAATGGAGA+AGG	+	chr6.1:75129609-75129628	MS.gene052439:CDS	40.0%
	AAAACAAGACTGGGAACCAG+TGG	+	chr6.1:75129255-75129274	MS.gene052439:CDS	45.0%
	ACAAGACCTTCGTAACCCAA+AGG	+	chr6.1:75129303-75129322	MS.gene052439:CDS	45.0%
	ACAGTCAAGAAACCAACAGC+CGG	+	chr6.1:75129364-75129383	MS.gene052439:CDS	45.0%
	ATTAGATGAAGCGGCTGAAC+CGG	+	chr6.1:75129432-75129451	MS.gene052439:CDS	45.0%
	CGCGAGGAAATTAGATGAAG+CGG	+	chr6.1:75129423-75129442	MS.gene052439:CDS	45.0%
	GAAGATGAGTCAGGGTGATT+TGG	+	chr6.1:75129516-75129535	MS.gene052439:CDS	45.0%
!	AAGGTCTTGTGCTTTGGGTT+TGG	-	chr6.1:75129293-75129312	None:intergenic	45.0%
!	GGGGAAAATGGAGAAGGTTT+TGG	+	chr6.1:75129615-75129634	MS.gene052439:CDS	45.0%
!	GTTACGAAGGTCTTGTGCTT+TGG	-	chr6.1:75129299-75129318	None:intergenic	45.0%
!	TTATGGCCTTTGGGTTACGA+AGG	-	chr6.1:75129312-75129331	None:intergenic	45.0%
!	TTTTTGTTCGAACCGGCTGT+TGG	-	chr6.1:75129379-75129398	None:intergenic	45.0%
!!!	GTGCTTTGGGTTTGGTTTTG+TGG	-	chr6.1:75129285-75129304	None:intergenic	45.0%
!!!	TTGGTTTTGTGGAGAACCAC+TGG	-	chr6.1:75129274-75129293	None:intergenic	45.0%
	ATAAACCAAGCCCTCCGAAC+CGG	+	chr6.1:75129328-75129347	MS.gene052439:CDS	50.0%
	ATTGTTCCCACGGTTCGTGT+CGG	-	chr6.1:75129235-75129254	None:intergenic	50.0%
	CAAAAAACCAACGAGCGGAC+CGG	+	chr6.1:75129393-75129412	MS.gene052439:CDS	50.0%
	GACTGTTAAGACCTCTGCTC+CGG	-	chr6.1:75129350-75129369	None:intergenic	50.0%
	TTAAGACCTCTGCTCCGGTT+CGG	-	chr6.1:75129345-75129364	None:intergenic	50.0%
!	AGGGCTTGGTTTATGGCCTT+TGG	-	chr6.1:75129322-75129341	None:intergenic	50.0%
!	GGGCTTGGTTTATGGCCTTT+GGG	-	chr6.1:75129321-75129340	None:intergenic	50.0%
!	TTTAAAACCGGTCCGCTCGT+TGG	-	chr6.1:75129403-75129422	None:intergenic	50.0%
!!	TGAGAGGGTTCAGGTTGTTC+AGG	+	chr6.1:75129552-75129571	MS.gene052439:CDS	50.0%
!	CGCGATACAGAAAGCGCGTT+TGG	+	chr6.1:75129489-75129508	MS.gene052439:CDS	55.0%
!	GGTTCGGAGGGCTTGGTTTA+TGG	-	chr6.1:75129329-75129348	None:intergenic	55.0%
!!!	GCGGACCGGTTTTAAACGCG+AGG	+	chr6.1:75129407-75129426	MS.gene052439:CDS	60.0%
!	CTGAACCGGCAGCGTTGGAG+CGG	+	chr6.1:75129446-75129465	MS.gene052439:CDS	65.0%
!	TGAACCGGCAGCGTTGGAGC+GGG	+	chr6.1:75129447-75129466	MS.gene052439:CDS	65.0%
!!	AGACCTCTGCTCCGGTTCGG+AGG	-	chr6.1:75129342-75129361	None:intergenic	65.0%
!!	GACCTCTGCTCCGGTTCGGA+GGG	-	chr6.1:75129341-75129360	None:intergenic	65.0%
	AGCCCTCCGAACCGGAGCAG+AGG	+	chr6.1:75129336-75129355	MS.gene052439:CDS	70.0%
	AGCGGCTGAACCGGCAGCGT+TGG	+	chr6.1:75129441-75129460	MS.gene052439:CDS	70.0%
	CCAACCCGCTCCAACGCTGC+CGG	-	chr6.1:75129454-75129473	None:intergenic	70.0%
!!	CTGCTCCGGTTCGGAGGGCT+TGG	-	chr6.1:75129336-75129355	None:intergenic	70.0%
!!	GCAGCGTTGGAGCGGGTTGG+TGG	+	chr6.1:75129454-75129473	MS.gene052439:CDS	70.0%
!	CCGGCAGCGTTGGAGCGGGT+TGG	+	chr6.1:75129451-75129470	MS.gene052439:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.1	gene	75129229	75129669	75129229	ID=MS.gene052439
chr6.1	mRNA	75129229	75129669	75129229	ID=MS.gene052439.t1;Parent=MS.gene052439
chr6.1	exon	75129229	75129669	75129229	ID=MS.gene052439.t1.exon1;Parent=MS.gene052439.t1
chr6.1	CDS	75129229	75129669	75129229	ID=cds.MS.gene052439.t1;Parent=MS.gene052439.t1

Gene Sequence

>MS.gene052439

ATGCCGACACGAACCGTGGGAACAATAAAACAAGACTGGGAACCAGTGGTTCTCCACAAAACCAAACCCAAAGCACAAGACCTTCGTAACCCAAAGGCCATAAACCAAGCCCTCCGAACCGGAGCAGAGGTCTTAACAGTCAAGAAACCAACAGCCGGTTCGAACAAAAAACCAACGAGCGGACCGGTTTTAAACGCGAGGAAATTAGATGAAGCGGCTGAACCGGCAGCGTTGGAGCGGGTTGGTGGAGAAGTGAGACACGCGATACAGAAAGCGCGTTTGGATAAGAAGATGAGTCAGGGTGATTTGGCGAAATTGATTAATGAGAGGGTTCAGGTTGTTCAGGAGTATGAGAATGGTAAAGCTGTTATTAATCAAGGTGTTTTGGGGAAAATGGAGAAGGTTTTGGGGGTTAAACTTAGGGGTAAAATTGGAAAATAA

Protein sequence

>MS.gene052439.t1

MPTRTVGTIKQDWEPVVLHKTKPKAQDLRNPKAINQALRTGAEVLTVKKPTAGSNKKPTSGPVLNARKLDEAAEPAALERVGGEVRHAIQKARLDKKMSQGDLAKLINERVQVVQEYENGKAVINQGVLGKMEKVLGVKLRGKIGK