Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56490.t1 | KEH30814.1 | 77.3 | 75 | 6 | 1 | 11 | 74 | 26 | 100 | 4.90E-09 | 70.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56490.t1 | A0A072UM07 | 77.3 | 75 | 6 | 1 | 11 | 74 | 26 | 100 | 3.5e-09 | 70.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049170 | MS.gene56490 | 0.852649 | 4.02E-61 | -1.69E-46 |
| MS.gene050060 | MS.gene56490 | 0.828096 | 1.09E-54 | -1.69E-46 |
| MS.gene050133 | MS.gene56490 | -0.839997 | 1.13E-57 | -1.69E-46 |
| MS.gene050404 | MS.gene56490 | 0.84554 | 3.81E-59 | -1.69E-46 |
| MS.gene050406 | MS.gene56490 | 0.850387 | 1.76E-60 | -1.69E-46 |
| MS.gene050426 | MS.gene56490 | -0.876885 | 9.78E-69 | -1.69E-46 |
| MS.gene050464 | MS.gene56490 | 0.806773 | 6.99E-50 | -1.69E-46 |
| MS.gene050529 | MS.gene56490 | 0.838107 | 3.50E-57 | -1.69E-46 |
| MS.gene050642 | MS.gene56490 | 0.817316 | 3.51E-52 | -1.69E-46 |
| MS.gene051171 | MS.gene56490 | 0.812202 | 4.77E-51 | -1.69E-46 |
| MS.gene051242 | MS.gene56490 | 0.802641 | 5.09E-49 | -1.69E-46 |
| MS.gene051983 | MS.gene56490 | 0.811304 | 7.49E-51 | -1.69E-46 |
| MS.gene052011 | MS.gene56490 | -0.811929 | 5.47E-51 | -1.69E-46 |
| MS.gene052077 | MS.gene56490 | 0.85669 | 2.71E-62 | -1.69E-46 |
| MS.gene052134 | MS.gene56490 | 0.874185 | 8.23E-68 | -1.69E-46 |
| MS.gene052135 | MS.gene56490 | 0.869908 | 2.18E-66 | -1.69E-46 |
| MS.gene052136 | MS.gene56490 | 0.818495 | 1.90E-52 | -1.69E-46 |
| MS.gene052188 | MS.gene56490 | 0.832769 | 7.82E-56 | -1.69E-46 |
| MS.gene052253 | MS.gene56490 | 0.803141 | 4.02E-49 | -1.69E-46 |
| MS.gene052310 | MS.gene56490 | 0.806602 | 7.59E-50 | -1.69E-46 |
| MS.gene052340 | MS.gene56490 | 0.860148 | 2.53E-63 | -1.69E-46 |
| MS.gene052414 | MS.gene56490 | 0.814462 | 1.52E-51 | -1.69E-46 |
| MS.gene052469 | MS.gene56490 | 0.856227 | 3.71E-62 | -1.69E-46 |
| MS.gene052590 | MS.gene56490 | 0.815619 | 8.43E-52 | -1.69E-46 |
| MS.gene052754 | MS.gene56490 | 0.806922 | 6.50E-50 | -1.69E-46 |
| MS.gene052758 | MS.gene56490 | 0.832855 | 7.45E-56 | -1.69E-46 |
| MS.gene053330 | MS.gene56490 | 0.886169 | 4.32E-72 | -1.69E-46 |
| MS.gene053557 | MS.gene56490 | 0.818782 | 1.64E-52 | -1.69E-46 |
| MS.gene054102 | MS.gene56490 | 0.819837 | 9.42E-53 | -1.69E-46 |
| MS.gene054110 | MS.gene56490 | 0.811068 | 8.42E-51 | -1.69E-46 |
| MS.gene054349 | MS.gene56490 | 0.85761 | 1.45E-62 | -1.69E-46 |
| MS.gene055116 | MS.gene56490 | 0.815356 | 9.64E-52 | -1.69E-46 |
| MS.gene055370 | MS.gene56490 | 0.841468 | 4.66E-58 | -1.69E-46 |
| MS.gene055539 | MS.gene56490 | 0.820026 | 8.52E-53 | -1.69E-46 |
| MS.gene05553 | MS.gene56490 | 0.864274 | 1.37E-64 | -1.69E-46 |
| MS.gene056633 | MS.gene56490 | 0.877798 | 4.71E-69 | -1.69E-46 |
| MS.gene056659 | MS.gene56490 | 0.832765 | 7.84E-56 | -1.69E-46 |
| MS.gene056664 | MS.gene56490 | 0.825054 | 5.78E-54 | -1.69E-46 |
| MS.gene056723 | MS.gene56490 | 0.803135 | 4.03E-49 | -1.69E-46 |
| MS.gene056899 | MS.gene56490 | -0.804629 | 1.97E-49 | -1.69E-46 |
| MS.gene056979 | MS.gene56490 | 0.801275 | 9.73E-49 | -1.69E-46 |
| MS.gene05702 | MS.gene56490 | 0.815543 | 8.76E-52 | -1.69E-46 |
| MS.gene057396 | MS.gene56490 | 0.827362 | 1.63E-54 | -1.69E-46 |
| MS.gene058142 | MS.gene56490 | 0.837666 | 4.54E-57 | -1.69E-46 |
| MS.gene058233 | MS.gene56490 | 0.808777 | 2.62E-50 | -1.69E-46 |
| MS.gene058276 | MS.gene56490 | 0.840216 | 9.94E-58 | -1.69E-46 |
| MS.gene058710 | MS.gene56490 | 0.819899 | 9.11E-53 | -1.69E-46 |
| MS.gene058743 | MS.gene56490 | 0.814273 | 1.68E-51 | -1.69E-46 |
| MS.gene058746 | MS.gene56490 | 0.860881 | 1.52E-63 | -1.69E-46 |
| MS.gene058928 | MS.gene56490 | 0.802529 | 5.37E-49 | -1.69E-46 |
| MS.gene059011 | MS.gene56490 | 0.833317 | 5.72E-56 | -1.69E-46 |
| MS.gene059247 | MS.gene56490 | 0.806994 | 6.28E-50 | -1.69E-46 |
| MS.gene059285 | MS.gene56490 | 0.847436 | 1.16E-59 | -1.69E-46 |
| MS.gene059511 | MS.gene56490 | 0.818762 | 1.66E-52 | -1.69E-46 |
| MS.gene059512 | MS.gene56490 | 0.810178 | 1.31E-50 | -1.69E-46 |
| MS.gene059717 | MS.gene56490 | 0.833284 | 5.83E-56 | -1.69E-46 |
| MS.gene05984 | MS.gene56490 | 0.803626 | 3.19E-49 | -1.69E-46 |
| MS.gene059894 | MS.gene56490 | 0.826589 | 2.50E-54 | -1.69E-46 |
| MS.gene060407 | MS.gene56490 | 0.83358 | 4.92E-56 | -1.69E-46 |
| MS.gene061049 | MS.gene56490 | 0.822725 | 2.03E-53 | -1.69E-46 |
| MS.gene061154 | MS.gene56490 | 0.807865 | 4.10E-50 | -1.69E-46 |
| MS.gene061159 | MS.gene56490 | 0.814293 | 1.66E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56490.t1 | MTR_4g082883 | 77.333 | 75 | 6 | 1 | 11 | 74 | 26 | 100 | 2.38e-32 | 107 |
| MS.gene56490.t1 | MTR_4g082900 | 55.172 | 58 | 26 | 0 | 16 | 73 | 24 | 81 | 8.77e-15 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCGGGTTTGGCTGGCTCTTC+TGG | 0.238096 | 4.4:+30142288 | None:intergenic |
| TTTGGCTGGCTCTTCTGGTT+TGG | 0.325140 | 4.4:+30142293 | None:intergenic |
| CTGGCTCTTCTGGTTTGGAC+TGG | 0.372877 | 4.4:+30142298 | None:intergenic |
| ACCAATCACACCTTCGGGTT+TGG | 0.373855 | 4.4:+30142275 | None:intergenic |
| AGCACCACCAATCACACCTT+CGG | 0.387425 | 4.4:+30142269 | None:intergenic |
| GCTTCAGCACAAACAACTAT+AGG | 0.435825 | 4.4:-30142384 | MS.gene56490:CDS |
| GCACCACCAATCACACCTTC+GGG | 0.442267 | 4.4:+30142270 | None:intergenic |
| AAAGAAACAATGGCATCAAC+TGG | 0.442857 | 4.4:-30142447 | None:intergenic |
| ATCACACCTTCGGGTTTGGC+TGG | 0.446616 | 4.4:+30142279 | None:intergenic |
| GCCAAACCCGAAGGTGTGAT+TGG | 0.484923 | 4.4:-30142276 | MS.gene56490:CDS |
| CTGGACAAAATCAGCAGCCT+TGG | 0.492880 | 4.4:+30142317 | None:intergenic |
| CTTGGTAGCATACTCTCCTA+CGG | 0.606883 | 4.4:+30142335 | None:intergenic |
| GAAGAGCCAGCCAAACCCGA+AGG | 0.622241 | 4.4:-30142285 | MS.gene56490:CDS |
| CGTAGGAGAGTATGCTACCA+AGG | 0.632082 | 4.4:-30142334 | MS.gene56490:CDS |
| GCTAATGTTCAGAAGACCGT+AGG | 0.649202 | 4.4:-30142351 | MS.gene56490:CDS |
| AAACCCGAAGGTGTGATTGG+TGG | 0.660272 | 4.4:-30142273 | MS.gene56490:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACCTGCCAAAAAATCGTTAT+TGG | + | chr4.4:30142255-30142274 | None:intergenic | 35.0% | |
| CCTGCCAAAAAATCGTTATT+GGG | + | chr4.4:30142254-30142273 | None:intergenic | 35.0% | |
| !!! | TGGACCCAATAACGATTTTT+TGG | - | chr4.4:30142247-30142266 | MS.gene56490:CDS | 35.0% |
| GCTTCAGCACAAACAACTAT+AGG | - | chr4.4:30142290-30142309 | MS.gene56490:CDS | 40.0% | |
| !!! | CCCAATAACGATTTTTTGGC+AGG | - | chr4.4:30142251-30142270 | MS.gene56490:CDS | 40.0% |
| GCTAATGTTCAGAAGACCGT+AGG | - | chr4.4:30142323-30142342 | MS.gene56490:CDS | 45.0% | |
| GGTGCTAAAAATGCTGTTGG+AGG | - | chr4.4:30142422-30142441 | MS.gene56490:CDS | 45.0% | |
| ! | CTTGGTAGCATACTCTCCTA+CGG | + | chr4.4:30142342-30142361 | None:intergenic | 45.0% |
| ! | GGTGGTGCTAAAAATGCTGT+TGG | - | chr4.4:30142419-30142438 | MS.gene56490:CDS | 45.0% |
| !!! | CGATTTTTTGGCAGGTGCAA+AGG | - | chr4.4:30142259-30142278 | MS.gene56490:CDS | 45.0% |
| AGCACCACCAATCACACCTT+CGG | + | chr4.4:30142408-30142427 | None:intergenic | 50.0% | |
| CTGGACAAAATCAGCAGCCT+TGG | + | chr4.4:30142360-30142379 | None:intergenic | 50.0% | |
| ! | AAACCCGAAGGTGTGATTGG+TGG | - | chr4.4:30142401-30142420 | MS.gene56490:CDS | 50.0% |
| ! | ACCAATCACACCTTCGGGTT+TGG | + | chr4.4:30142402-30142421 | None:intergenic | 50.0% |
| ! | CGTAGGAGAGTATGCTACCA+AGG | - | chr4.4:30142340-30142359 | MS.gene56490:CDS | 50.0% |
| ! | TTTGGCTGGCTCTTCTGGTT+TGG | + | chr4.4:30142384-30142403 | None:intergenic | 50.0% |
| GCACCACCAATCACACCTTC+GGG | + | chr4.4:30142407-30142426 | None:intergenic | 55.0% | |
| ! | ATCACACCTTCGGGTTTGGC+TGG | + | chr4.4:30142398-30142417 | None:intergenic | 55.0% |
| ! | GCCAAACCCGAAGGTGTGAT+TGG | - | chr4.4:30142398-30142417 | MS.gene56490:CDS | 55.0% |
| !! | CTGGCTCTTCTGGTTTGGAC+TGG | + | chr4.4:30142379-30142398 | None:intergenic | 55.0% |
| GAAGAGCCAGCCAAACCCGA+AGG | - | chr4.4:30142389-30142408 | MS.gene56490:CDS | 60.0% | |
| ! | TCGGGTTTGGCTGGCTCTTC+TGG | + | chr4.4:30142389-30142408 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 30142236 | 30142460 | 30142236 | ID=MS.gene56490 |
| chr4.4 | mRNA | 30142236 | 30142460 | 30142236 | ID=MS.gene56490.t1;Parent=MS.gene56490 |
| chr4.4 | exon | 30142236 | 30142460 | 30142236 | ID=MS.gene56490.t1.exon1;Parent=MS.gene56490.t1 |
| chr4.4 | CDS | 30142236 | 30142460 | 30142236 | ID=cds.MS.gene56490.t1;Parent=MS.gene56490.t1 |
| Gene Sequence |
| Protein sequence |
