AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34190


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34190.t1 MTR_6g007180 97.849 93 2 0 1 93 208 300 9.29e-62 189
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34190.t1 AT3G55250 56.044 91 39 1 1 91 185 274 8.19e-24 91.7

Find 15 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATCTACGTTTGTTGCTT+CGG 0.330000 6.2:-2745777 MS.gene34190:CDS
AGAGATTCGGCGCATGGTTA+AGG 0.388940 6.2:-2745676 MS.gene34190:CDS
TCAACAGCTTCCTTTGATAG+AGG 0.406723 6.2:+2745597 None:intergenic
CTCTCATCTTCTGTCTCTAT+AGG 0.411361 6.2:+2745747 None:intergenic
GATAGAGGCTTAGCAGCATC+TGG 0.416100 6.2:+2745612 None:intergenic
GAAAGTAGAAGACGGAGGTA+TGG 0.445392 6.2:-2745643 MS.gene34190:CDS
TTCTCAACTCGAACCTCCAT+TGG 0.453422 6.2:+2745810 None:intergenic
AAAACAAGAGATTCGGCGCA+TGG 0.513137 6.2:-2745682 MS.gene34190:CDS
CTACGTTTGTTGCTTCGGAA+AGG 0.538343 6.2:-2745772 MS.gene34190:CDS
TCGGCGCATGGTTAAGGAGA+TGG 0.565322 6.2:-2745670 MS.gene34190:CDS
TGCTGCTAAGCCTCTATCAA+AGG 0.573332 6.2:-2745607 MS.gene34190:CDS
AATGGAGGTTCGAGTTGAGA+AGG 0.577559 6.2:-2745808 MS.gene34190:CDS
CTATAGAGACAGAAGATGAG+AGG 0.617615 6.2:-2745746 MS.gene34190:CDS
ATGGCAGAGAAAGTAGAAGA+CGG 0.643691 6.2:-2745651 MS.gene34190:CDS
GCAGAGAAAGTAGAAGACGG+AGG 0.701413 6.2:-2745648 MS.gene34190:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTGTTTGAGAAAGTTA+AGG + chr6.2:2745799-2745818 None:intergenic 20.0%
CAGTTCAAAAACAAGAGATT+CGG - chr6.2:2745666-2745685 MS.gene34190:CDS 30.0%
!!! ATCTCTTGTTTTTGAACTGA+TGG + chr6.2:2745665-2745684 None:intergenic 30.0%
ATGGCAGAGAAAGTAGAAGA+CGG - chr6.2:2745704-2745723 MS.gene34190:CDS 40.0%
CTATAGAGACAGAAGATGAG+AGG - chr6.2:2745609-2745628 MS.gene34190:CDS 40.0%
CTCTCATCTTCTGTCTCTAT+AGG + chr6.2:2745611-2745630 None:intergenic 40.0%
TCAACAGCTTCCTTTGATAG+AGG + chr6.2:2745761-2745780 None:intergenic 40.0%
! GCAATCTACGTTTGTTGCTT+CGG - chr6.2:2745578-2745597 MS.gene34190:CDS 40.0%
AAAACAAGAGATTCGGCGCA+TGG - chr6.2:2745673-2745692 MS.gene34190:CDS 45.0%
GAAAGTAGAAGACGGAGGTA+TGG - chr6.2:2745712-2745731 MS.gene34190:CDS 45.0%
TGCTGCTAAGCCTCTATCAA+AGG - chr6.2:2745748-2745767 MS.gene34190:CDS 45.0%
! CTACGTTTGTTGCTTCGGAA+AGG - chr6.2:2745583-2745602 MS.gene34190:CDS 45.0%
AGAGATTCGGCGCATGGTTA+AGG - chr6.2:2745679-2745698 MS.gene34190:CDS 50.0%
GATAGAGGCTTAGCAGCATC+TGG + chr6.2:2745746-2745765 None:intergenic 50.0%
GCAGAGAAAGTAGAAGACGG+AGG - chr6.2:2745707-2745726 MS.gene34190:CDS 50.0%
TCGGCGCATGGTTAAGGAGA+TGG - chr6.2:2745685-2745704 MS.gene34190:CDS 55.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 2745548 2745829 2745548 ID=MS.gene34190
chr6.2 mRNA 2745548 2745829 2745548 ID=MS.gene34190.t1;Parent=MS.gene34190
chr6.2 exon 2745548 2745829 2745548 ID=MS.gene34190.t1.exon1;Parent=MS.gene34190.t1
chr6.2 CDS 2745548 2745829 2745548 ID=cds.MS.gene34190.t1;Parent=MS.gene34190.t1
Gene Sequence

>MS.gene34190

ATGGAGGTTCGAGTTGAGAAGGCTCTTGATGCAATCTACGTTTGTTGCTTCGGAAAGGATCCTATAGAGACAGAAGATGAGAGGTTGCTGAGTACAATACTTAGTTCAGTTTTTCCATCAGTTCAAAAACAAGAGATTCGGCGCATGGTTAAGGAGATGGCAGAGAAAGTAGAAGACGGAGGTATGGATTATATTCCAGATGCTGCTAAGCCTCTATCAAAGGAAGCTGTTGAAATGCAAATGAAAGACCTTAACTTTCTCAAACAAAATAGTAACAATTAG

Protein sequence

>MS.gene34190.t1

MEVRVEKALDAIYVCCFGKDPIETEDERLLSTILSSVFPSVQKQEIRRMVKEMAEKVEDGGMDYIPDAAKPLSKEAVEMQMKDLNFLKQNSNN