Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93738.t1 | XP_024629046.1 | 96.2 | 421 | 16 | 0 | 1 | 421 | 1 | 421 | 7.70E-208 | 733 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93738.t1 | Q9LVJ0 | 70.0 | 337 | 92 | 5 | 87 | 421 | 92 | 421 | 3.5e-124 | 446.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93738.t1 | A0A396GSD0 | 96.2 | 421 | 16 | 0 | 1 | 421 | 1 | 421 | 5.5e-208 | 733.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049232 | MS.gene93738 | 0.808984 | 2.37E-50 | -1.69E-46 |
| MS.gene049396 | MS.gene93738 | 0.811275 | 7.60E-51 | -1.69E-46 |
| MS.gene05010 | MS.gene93738 | 0.80482 | 1.80E-49 | -1.69E-46 |
| MS.gene050170 | MS.gene93738 | 0.891246 | 4.73E-74 | -1.69E-46 |
| MS.gene050323 | MS.gene93738 | 0.882514 | 9.78E-71 | -1.69E-46 |
| MS.gene050338 | MS.gene93738 | -0.806357 | 8.56E-50 | -1.69E-46 |
| MS.gene050496 | MS.gene93738 | 0.848241 | 6.95E-60 | -1.69E-46 |
| MS.gene050502 | MS.gene93738 | 0.805904 | 1.07E-49 | -1.69E-46 |
| MS.gene050697 | MS.gene93738 | 0.889463 | 2.37E-73 | -1.69E-46 |
| MS.gene050868 | MS.gene93738 | 0.849739 | 2.66E-60 | -1.69E-46 |
| MS.gene050871 | MS.gene93738 | 0.854178 | 1.46E-61 | -1.69E-46 |
| MS.gene050904 | MS.gene93738 | 0.818636 | 1.77E-52 | -1.69E-46 |
| MS.gene051268 | MS.gene93738 | 0.849333 | 3.46E-60 | -1.69E-46 |
| MS.gene051838 | MS.gene93738 | 0.817122 | 3.88E-52 | -1.69E-46 |
| MS.gene051941 | MS.gene93738 | 0.819945 | 8.90E-53 | -1.69E-46 |
| MS.gene052066 | MS.gene93738 | 0.802444 | 5.59E-49 | -1.69E-46 |
| MS.gene052100 | MS.gene93738 | 0.814174 | 1.76E-51 | -1.69E-46 |
| MS.gene052822 | MS.gene93738 | 0.806876 | 6.65E-50 | -1.69E-46 |
| MS.gene052823 | MS.gene93738 | 0.800394 | 1.47E-48 | -1.69E-46 |
| MS.gene053162 | MS.gene93738 | 0.807222 | 5.62E-50 | -1.69E-46 |
| MS.gene053479 | MS.gene93738 | 0.81798 | 2.49E-52 | -1.69E-46 |
| MS.gene053480 | MS.gene93738 | 0.822652 | 2.11E-53 | -1.69E-46 |
| MS.gene053626 | MS.gene93738 | 0.800552 | 1.37E-48 | -1.69E-46 |
| MS.gene053633 | MS.gene93738 | 0.830757 | 2.45E-55 | -1.69E-46 |
| MS.gene053824 | MS.gene93738 | 0.825406 | 4.77E-54 | -1.69E-46 |
| MS.gene054614 | MS.gene93738 | -0.803068 | 4.16E-49 | -1.69E-46 |
| MS.gene054941 | MS.gene93738 | -0.834787 | 2.45E-56 | -1.69E-46 |
| MS.gene05494 | MS.gene93738 | 0.819662 | 1.03E-52 | -1.69E-46 |
| MS.gene054954 | MS.gene93738 | 0.802468 | 5.53E-49 | -1.69E-46 |
| MS.gene055748 | MS.gene93738 | 0.804 | 2.67E-49 | -1.69E-46 |
| MS.gene055750 | MS.gene93738 | 0.817991 | 2.47E-52 | -1.69E-46 |
| MS.gene055751 | MS.gene93738 | 0.818088 | 2.35E-52 | -1.69E-46 |
| MS.gene055776 | MS.gene93738 | 0.810963 | 8.88E-51 | -1.69E-46 |
| MS.gene055847 | MS.gene93738 | 0.813243 | 2.83E-51 | -1.69E-46 |
| MS.gene05589 | MS.gene93738 | 0.864065 | 1.59E-64 | -1.69E-46 |
| MS.gene056046 | MS.gene93738 | 0.847735 | 9.58E-60 | -1.69E-46 |
| MS.gene056387 | MS.gene93738 | 0.928954 | 1.39E-92 | -1.69E-46 |
| MS.gene056504 | MS.gene93738 | 0.863673 | 2.11E-64 | -1.69E-46 |
| MS.gene056618 | MS.gene93738 | -0.847615 | 1.03E-59 | -1.69E-46 |
| MS.gene057086 | MS.gene93738 | 0.816489 | 5.39E-52 | -1.69E-46 |
| MS.gene05787 | MS.gene93738 | 0.830649 | 2.61E-55 | -1.69E-46 |
| MS.gene058157 | MS.gene93738 | 0.827927 | 1.19E-54 | -1.69E-46 |
| MS.gene058382 | MS.gene93738 | -0.803165 | 3.97E-49 | -1.69E-46 |
| MS.gene059667 | MS.gene93738 | 0.828827 | 7.24E-55 | -1.69E-46 |
| MS.gene059703 | MS.gene93738 | -0.812085 | 5.06E-51 | -1.69E-46 |
| MS.gene059837 | MS.gene93738 | 0.801717 | 7.89E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene93738 | 0.83074 | 2.48E-55 | -1.69E-46 |
| MS.gene05995 | MS.gene93738 | 0.830743 | 2.47E-55 | -1.69E-46 |
| MS.gene06000 | MS.gene93738 | 0.864734 | 9.83E-65 | -1.69E-46 |
| MS.gene060778 | MS.gene93738 | 0.865493 | 5.67E-65 | -1.69E-46 |
| MS.gene06097 | MS.gene93738 | 0.833585 | 4.90E-56 | -1.69E-46 |
| MS.gene060982 | MS.gene93738 | 0.856637 | 2.81E-62 | -1.69E-46 |
| MS.gene061218 | MS.gene93738 | 0.884805 | 1.40E-71 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene72908 | MS.gene93738 | PPI |
| MS.gene93738 | MS.gene028007 | PPI |
| MS.gene98452 | MS.gene93738 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93738.t1 | MTR_6g071465 | 64.377 | 393 | 124 | 5 | 30 | 421 | 41 | 418 | 2.59e-165 | 471 |
| MS.gene93738.t1 | MTR_5g028020 | 44.651 | 430 | 196 | 11 | 4 | 419 | 7 | 408 | 1.72e-110 | 331 |
| MS.gene93738.t1 | MTR_8g012420 | 31.880 | 367 | 191 | 10 | 74 | 416 | 36 | 367 | 6.09e-51 | 176 |
| MS.gene93738.t1 | MTR_7g053530 | 28.134 | 359 | 198 | 10 | 76 | 416 | 21 | 337 | 3.35e-28 | 114 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93738.t1 | AT3G17800 | 60.641 | 437 | 140 | 10 | 1 | 421 | 1 | 421 | 6.46e-163 | 465 |
| MS.gene93738.t1 | AT3G17800 | 67.680 | 362 | 108 | 5 | 62 | 421 | 73 | 427 | 2.85e-162 | 463 |
| MS.gene93738.t1 | AT1G48450 | 64.130 | 368 | 117 | 5 | 62 | 421 | 63 | 423 | 5.33e-162 | 462 |
| MS.gene93738.t1 | AT1G48450 | 59.176 | 267 | 94 | 5 | 62 | 320 | 63 | 322 | 3.09e-100 | 302 |
| MS.gene93738.t1 | AT1G48450 | 58.303 | 271 | 98 | 5 | 62 | 324 | 63 | 326 | 3.21e-99 | 300 |
| MS.gene93738.t1 | AT1G32160 | 43.392 | 401 | 175 | 11 | 36 | 419 | 39 | 404 | 1.50e-98 | 300 |
| MS.gene93738.t1 | AT3G07310 | 35.870 | 368 | 187 | 10 | 59 | 421 | 33 | 356 | 1.75e-54 | 185 |
| MS.gene93738.t1 | AT3G07310 | 36.842 | 285 | 135 | 8 | 142 | 421 | 8 | 252 | 3.58e-45 | 157 |
| MS.gene93738.t1 | AT5G48590 | 33.149 | 362 | 168 | 8 | 60 | 416 | 36 | 328 | 4.68e-45 | 159 |
Find 125 sgRNAs with CRISPR-Local
Find 150 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATTAGCTTTGGTGGTTTA+AGG | 0.185413 | 4.2:-97494 | MS.gene93738:CDS |
| CTTTCCTCCTTCTAATAATA+AGG | 0.195362 | 4.2:-98909 | MS.gene93738:intron |
| GAATGAGAGCCAAGTGGTTT+TGG | 0.255570 | 4.2:+98000 | None:intergenic |
| AACCACTTGGCTCTCATTCT+TGG | 0.279367 | 4.2:-97996 | MS.gene93738:CDS |
| CTCACGTGTGATAACCATTC+CGG | 0.313242 | 4.2:+97551 | None:intergenic |
| GATAGTACCTTATTATTAGA+AGG | 0.339689 | 4.2:+98902 | None:intergenic |
| ATCTTATTGTTGCGTATCTC+TGG | 0.348662 | 4.2:+97628 | None:intergenic |
| AAGCACACAGAGGCATTGTT+TGG | 0.370683 | 4.2:-97579 | MS.gene93738:CDS |
| TCGACCCTACCAGTTGCATC+TGG | 0.375048 | 4.2:+98086 | None:intergenic |
| ACTTCTCCAGCTGGAACCTT+TGG | 0.389283 | 4.2:+97868 | None:intergenic |
| GCAATCTTATGAGAAGAGTC+TGG | 0.407257 | 4.2:+98492 | None:intergenic |
| GAAACCATCAAAATTAGCTT+TGG | 0.409557 | 4.2:-97504 | MS.gene93738:CDS |
| ATGTGGTAGGAGAAGGATCT+TGG | 0.410740 | 4.2:+98331 | None:intergenic |
| ATACGCAACAATAAGATCTA+AGG | 0.415923 | 4.2:-97622 | MS.gene93738:CDS |
| GATGTCAGTCCTGGTGGATT+TGG | 0.419214 | 4.2:-97711 | MS.gene93738:CDS |
| ATTGGTTGTGCAAAAGTTCA+TGG | 0.419257 | 4.2:-98141 | MS.gene93738:CDS |
| ACCATCAAAATTAGCTTTGG+TGG | 0.419551 | 4.2:-97501 | MS.gene93738:CDS |
| CCACCGCAGCAGGCACAAGA+TGG | 0.439006 | 4.2:+98409 | None:intergenic |
| CCAGGCCAAGTAGATACTTC+AGG | 0.441052 | 4.2:+97738 | None:intergenic |
| CAACTCAATTTAAGACGAAA+TGG | 0.445619 | 4.2:+97400 | None:intergenic |
| AGTTGCTGGTCCACCGCAGC+AGG | 0.446973 | 4.2:+98399 | None:intergenic |
| GTGAAGAAGGCGGCAGGATC+CGG | 0.448164 | 4.2:+98931 | None:intergenic |
| CCATCTTGTGCCTGCTGCGG+TGG | 0.450747 | 4.2:-98409 | MS.gene93738:CDS |
| CTCATCCTGAAGTATCTACT+TGG | 0.453032 | 4.2:-97743 | MS.gene93738:CDS |
| TCACGTGTGATAACCATTCC+GGG | 0.453796 | 4.2:+97552 | None:intergenic |
| TCATGCTTATGCCTAGGGAT+GGG | 0.466457 | 4.2:+98615 | None:intergenic |
| CAGGAGAATTGCTGAGGTCA+AGG | 0.467848 | 4.2:-98204 | MS.gene93738:CDS |
| CCGTCTTCTTCAATTACAAC+CGG | 0.472072 | 4.2:-98950 | MS.gene93738:CDS |
| CTTCAGGATGAGATGTCACT+TGG | 0.474439 | 4.2:+97754 | None:intergenic |
| ATTGGAGCTGGAGAAGTTGC+TGG | 0.477347 | 4.2:+98385 | None:intergenic |
| TAGAAGGAGGAAAGTGAAGA+AGG | 0.482490 | 4.2:+98918 | None:intergenic |
| CATGCTTATGCCTAGGGATG+GGG | 0.483080 | 4.2:+98616 | None:intergenic |
| AGTTGCATCTGGGGTTATAG+AGG | 0.486143 | 4.2:+98097 | None:intergenic |
| CACCGCAGCAGGCACAAGAT+GGG | 0.488056 | 4.2:+98410 | None:intergenic |
| AAGAAGACGGCGGTGATAAG+AGG | 0.488970 | 4.2:+98963 | None:intergenic |
| TGGGTCTGGCGAGGAGGAAG+AGG | 0.490825 | 4.2:+98512 | None:intergenic |
| GATTCATGGTCAAATGAAGA+TGG | 0.491027 | 4.2:-98065 | MS.gene93738:CDS |
| CTCATGCTTATGCCTAGGGA+TGG | 0.491888 | 4.2:+98614 | None:intergenic |
| CTCATTCTTGGTAACAGAGC+AGG | 0.493044 | 4.2:-97984 | MS.gene93738:CDS |
| AAATGAAAGGAGGACAGCTC+TGG | 0.495873 | 4.2:-98180 | MS.gene93738:CDS |
| TCTATAACCCCAGATGCAAC+TGG | 0.497553 | 4.2:-98095 | MS.gene93738:CDS |
| CAACTGGTAGGGTCGATTCA+TGG | 0.499248 | 4.2:-98079 | MS.gene93738:CDS |
| CGACCCTACCAGTTGCATCT+GGG | 0.501121 | 4.2:+98087 | None:intergenic |
| CCTGGTGGCGATGTCAGTCC+TGG | 0.502730 | 4.2:-97720 | MS.gene93738:CDS |
| CCTGAAGTATCTACTTGGCC+TGG | 0.503117 | 4.2:-97738 | MS.gene93738:CDS |
| CAAATTAGCAAACTGAGGAT+AGG | 0.505986 | 4.2:-97942 | MS.gene93738:CDS |
| TTATGAGAAGAGTCTGGGTC+TGG | 0.513057 | 4.2:+98498 | None:intergenic |
| GAGCAACATCACGATCAGAT+TGG | 0.514423 | 4.2:+98367 | None:intergenic |
| AACTATGTGATGTCCTTTGA+CGG | 0.517188 | 4.2:-97660 | MS.gene93738:CDS |
| GTCTCTCATGCTTATGCCTA+GGG | 0.517855 | 4.2:+98610 | None:intergenic |
| CAAAACAAGATCGGAGGATG+TGG | 0.519960 | 4.2:+98314 | None:intergenic |
| AAATTAGCAAACTGAGGATA+GGG | 0.522002 | 4.2:-97941 | MS.gene93738:CDS |
| TGAGAAAGAAACTGACCGAT+TGG | 0.524356 | 4.2:+98450 | None:intergenic |
| CTTCAGTTTCAGTCTCCAAT+CGG | 0.525591 | 4.2:-98465 | MS.gene93738:CDS |
| AGGAAAGTGAAGAAGGCGGC+AGG | 0.526126 | 4.2:+98925 | None:intergenic |
| TGATAAGAGGAGGACGGCTG+TGG | 0.526370 | 4.2:+98976 | None:intergenic |
| ACCGCAGCAGGCACAAGATG+GGG | 0.529440 | 4.2:+98411 | None:intergenic |
| AGCATGAGAGACTCTCTGTT+GGG | 0.532872 | 4.2:-98599 | MS.gene93738:CDS |
| TATGCAGCATCAGTGATGTA+TGG | 0.532897 | 4.2:-97912 | MS.gene93738:CDS |
| CAGTTTGCTAATTTGAGCAA+CGG | 0.532928 | 4.2:+97950 | None:intergenic |
| CATCACGATCAGATTGGAGC+TGG | 0.536635 | 4.2:+98373 | None:intergenic |
| CTGGAGGGTATCACCGTCAA+AGG | 0.539393 | 4.2:+97647 | None:intergenic |
| GAAGTGTCCACTTCACTACC+AGG | 0.539689 | 4.2:+97777 | None:intergenic |
| AGTCTCTCATGCTTATGCCT+AGG | 0.540729 | 4.2:+98609 | None:intergenic |
| GCACTTCCGTCCCCATCCCT+AGG | 0.541488 | 4.2:-98626 | MS.gene93738:CDS |
| AGTCCTGGTGGATTTGGCTA+TGG | 0.543579 | 4.2:-97705 | MS.gene93738:CDS |
| TGATGAATACTACTGTCGTC+TGG | 0.547484 | 4.2:+97822 | None:intergenic |
| TTATTGTTGCGTATCTCTGG+AGG | 0.549788 | 4.2:+97631 | None:intergenic |
| CCAGGACTGACATCGCCACC+AGG | 0.556642 | 4.2:+97720 | None:intergenic |
| ATTGTTTGGAAGACCCGGAA+TGG | 0.558540 | 4.2:-97565 | MS.gene93738:CDS |
| AGTTGACCAAAGGTTCCAGC+TGG | 0.559976 | 4.2:-97874 | MS.gene93738:CDS |
| ATTCATGGTCAAATGAAGAT+GGG | 0.560883 | 4.2:-98064 | MS.gene93738:CDS |
| TCGGAGGATGTGGTAGGAGA+AGG | 0.562860 | 4.2:+98324 | None:intergenic |
| TTCTTAAGCGAGTTGACCAA+AGG | 0.564871 | 4.2:-97884 | MS.gene93738:CDS |
| CTTTCATCGACTTCTCCAGC+TGG | 0.565573 | 4.2:+97859 | None:intergenic |
| CAATCTTATGAGAAGAGTCT+GGG | 0.567804 | 4.2:+98493 | None:intergenic |
| GAGGCATTGTTTGGAAGACC+CGG | 0.570792 | 4.2:-97570 | MS.gene93738:CDS |
| CTAACCTAGCCGTATGGACG+TGG | 0.574142 | 4.2:-99002 | None:intergenic |
| AAGCATGAGAGACTCTCTGT+TGG | 0.574311 | 4.2:-98600 | MS.gene93738:CDS |
| CATCACATAGTTACGCAACC+TGG | 0.581846 | 4.2:+97671 | None:intergenic |
| TAGTATTCATCAAACTATTG+TGG | 0.584247 | 4.2:-97811 | MS.gene93738:CDS |
| TAGCAAACTGAGGATAGGGC+AGG | 0.587895 | 4.2:-97937 | MS.gene93738:CDS |
| GACCCTACCAGTTGCATCTG+GGG | 0.591341 | 4.2:+98088 | None:intergenic |
| AACCCCAGATGCAACTGGTA+GGG | 0.592069 | 4.2:-98090 | MS.gene93738:CDS |
| AGAGTCTGGGTCTGGCGAGG+AGG | 0.592281 | 4.2:+98506 | None:intergenic |
| TAACCCCAGATGCAACTGGT+AGG | 0.592551 | 4.2:-98091 | MS.gene93738:CDS |
| GTGCTTCTCAATAATGCTCA+CGG | 0.595139 | 4.2:+97596 | None:intergenic |
| TATTGTTGCGTATCTCTGGA+GGG | 0.599151 | 4.2:+97632 | None:intergenic |
| GGAAGAGATCTTGTACACAT+TGG | 0.602232 | 4.2:-98159 | MS.gene93738:CDS |
| AGTACCTTATTATTAGAAGG+AGG | 0.610361 | 4.2:+98905 | None:intergenic |
| CCGCAGCAGGCACAAGATGG+GGG | 0.617352 | 4.2:+98412 | None:intergenic |
| AATGGTTATCACACGTGAGG+GGG | 0.623377 | 4.2:-97547 | MS.gene93738:CDS |
| CGCAACAATAAGATCTAAGG+AGG | 0.623407 | 4.2:-97619 | MS.gene93738:CDS |
| AGAAGAGTCTGGGTCTGGCG+AGG | 0.623678 | 4.2:+98503 | None:intergenic |
| ATACCATAGCCAAATCCACC+AGG | 0.624211 | 4.2:+97702 | None:intergenic |
| GGAATGGTTATCACACGTGA+GGG | 0.627905 | 4.2:-97549 | MS.gene93738:CDS |
| GTTGCATCTGGGGTTATAGA+GGG | 0.629178 | 4.2:+98098 | None:intergenic |
| ACAAGATCGGAGGATGTGGT+AGG | 0.629560 | 4.2:+98318 | None:intergenic |
| TTTGCTAATTTGAGCAACGG+AGG | 0.631175 | 4.2:+97953 | None:intergenic |
| ATTGGAGACTGAAACTGAAG+AGG | 0.632400 | 4.2:+98468 | None:intergenic |
| AAACCTGTACAAAACAAGAT+CGG | 0.634696 | 4.2:+98305 | None:intergenic |
| GGTGGCGATGTCAGTCCTGG+TGG | 0.636075 | 4.2:-97717 | MS.gene93738:CDS |
| TTCAGGATGAGATGTCACTT+GGG | 0.636764 | 4.2:+97755 | None:intergenic |
| CGGAATGGTTATCACACGTG+AGG | 0.638347 | 4.2:-97550 | MS.gene93738:CDS |
| TGAGATGATCCAAAACCACT+TGG | 0.639402 | 4.2:-98009 | MS.gene93738:CDS |
| CTGAGGTCAAGGCAAATGAA+AGG | 0.640671 | 4.2:-98193 | MS.gene93738:CDS |
| GCTATGGTATAAAGCCGACC+AGG | 0.641906 | 4.2:-97689 | MS.gene93738:CDS |
| TGCAAGACCTGGTAGTGAAG+TGG | 0.643713 | 4.2:-97784 | MS.gene93738:CDS |
| TTGAGAGCTACGTGGACTCG+AGG | 0.645363 | 4.2:-97425 | MS.gene93738:CDS |
| AAGACGGCGGTGATAAGAGG+AGG | 0.647164 | 4.2:+98966 | None:intergenic |
| CCGGTTGTAATTGAAGAAGA+CGG | 0.652339 | 4.2:+98950 | None:intergenic |
| GAAGTATCTACTTGGCCTGG+TGG | 0.652940 | 4.2:-97735 | MS.gene93738:CDS |
| GAATGGTTATCACACGTGAG+GGG | 0.665737 | 4.2:-97548 | MS.gene93738:CDS |
| ACATAGTTACGCAACCTGGT+CGG | 0.667626 | 4.2:+97675 | None:intergenic |
| CCTGTACAAAACAAGATCGG+AGG | 0.668029 | 4.2:+98308 | None:intergenic |
| TACCAAGAATGAGAGCCAAG+TGG | 0.671508 | 4.2:+97994 | None:intergenic |
| CGGCGGTGATAAGAGGAGGA+CGG | 0.675716 | 4.2:+98970 | None:intergenic |
| CTTATGCCTAGGGATGGGGA+CGG | 0.676237 | 4.2:+98620 | None:intergenic |
| CTGGGATGTTGAGAGCTACG+TGG | 0.676347 | 4.2:-97433 | MS.gene93738:CDS |
| AGGTCAAGGCAAATGAAAGG+AGG | 0.687801 | 4.2:-98190 | MS.gene93738:CDS |
| ATTGTGGATGATGCAAGACC+TGG | 0.688318 | 4.2:-97795 | MS.gene93738:CDS |
| TTGCTCAAATTAGCAAACTG+AGG | 0.691447 | 4.2:-97947 | MS.gene93738:CDS |
| AAGGAGGAAAGTGAAGAAGG+CGG | 0.701835 | 4.2:+98921 | None:intergenic |
| CATTATTGAGAAGCACACAG+AGG | 0.764856 | 4.2:-97589 | MS.gene93738:CDS |
| GTTGTAATTGAAGAAGACGG+CGG | 0.772972 | 4.2:+98953 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTGCAAGATATTATACA+GGG | + | chr4.2:97683-97702 | None:intergenic | 20.0% |
| !! | TAAATTGCAAGATATTATAC+AGG | + | chr4.2:97684-97703 | None:intergenic | 20.0% |
| ! | GATAGTACCTTATTATTAGA+AGG | + | chr4.2:97494-97513 | None:intergenic | 25.0% |
| ! | TAGTATTCATCAAACTATTG+TGG | - | chr4.2:98582-98601 | MS.gene93738:CDS | 25.0% |
| ! | TCATATATTCTGTTTGTTTG+TGG | + | chr4.2:97716-97735 | None:intergenic | 25.0% |
| AAACCTGTACAAAACAAGAT+CGG | + | chr4.2:98091-98110 | None:intergenic | 30.0% | |
| AAATTAGCAAACTGAGGATA+GGG | - | chr4.2:98452-98471 | MS.gene93738:CDS | 30.0% | |
| AGTACCTTATTATTAGAAGG+AGG | + | chr4.2:97491-97510 | None:intergenic | 30.0% | |
| ATACGCAACAATAAGATCTA+AGG | - | chr4.2:98771-98790 | MS.gene93738:intron | 30.0% | |
| ATGAACAAGCAAGAAATAGT+AGG | + | chr4.2:98138-98157 | None:intergenic | 30.0% | |
| ATTCATGGTCAAATGAAGAT+GGG | - | chr4.2:98329-98348 | MS.gene93738:CDS | 30.0% | |
| CTTTCCTCCTTCTAATAATA+AGG | - | chr4.2:97484-97503 | MS.gene93738:CDS | 30.0% | |
| GACAATAGATTGATGAATTG+AGG | + | chr4.2:98116-98135 | None:intergenic | 30.0% | |
| TAATAATTACCACTAGTTGC+TGG | - | chr4.2:97593-97612 | MS.gene93738:CDS | 30.0% | |
| TGAAATATTGATCCATTCCT+TGG | - | chr4.2:97733-97752 | MS.gene93738:CDS | 30.0% | |
| ! | ACCTAAACAAAGCATTAGTT+TGG | + | chr4.2:97627-97646 | None:intergenic | 30.0% |
| ! | GAAACCATCAAAATTAGCTT+TGG | - | chr4.2:98889-98908 | MS.gene93738:intron | 30.0% |
| !! | AAATTAGCTTTGGTGGTTTA+AGG | - | chr4.2:98899-98918 | MS.gene93738:intron | 30.0% |
| !! | ACCAAACTAATGCTTTGTTT+AGG | - | chr4.2:97623-97642 | MS.gene93738:CDS | 30.0% |
| !! | ACTATCTTCTCAACTTTTTC+AGG | - | chr4.2:98170-98189 | MS.gene93738:CDS | 30.0% |
| !!! | TTTAAGGAGACTTGTTTTAG+AGG | - | chr4.2:98915-98934 | MS.gene93738:CDS | 30.0% |
| AACTATGTGATGTCCTTTGA+CGG | - | chr4.2:98733-98752 | MS.gene93738:intron | 35.0% | |
| AAGTTGAGAAGATAGTGCAA+TGG | + | chr4.2:98166-98185 | None:intergenic | 35.0% | |
| ATATTGATCCATTCCTTGGT+TGG | - | chr4.2:97737-97756 | MS.gene93738:CDS | 35.0% | |
| ATCTTATTGTTGCGTATCTC+TGG | + | chr4.2:98768-98787 | None:intergenic | 35.0% | |
| ATTGGTTGTGCAAAAGTTCA+TGG | - | chr4.2:98252-98271 | MS.gene93738:intron | 35.0% | |
| CAAATTAGCAAACTGAGGAT+AGG | - | chr4.2:98451-98470 | MS.gene93738:CDS | 35.0% | |
| CAATCTTATGAGAAGAGTCT+GGG | + | chr4.2:97903-97922 | None:intergenic | 35.0% | |
| GATTCATGGTCAAATGAAGA+TGG | - | chr4.2:98328-98347 | MS.gene93738:CDS | 35.0% | |
| TTGCTCAAATTAGCAAACTG+AGG | - | chr4.2:98446-98465 | MS.gene93738:CDS | 35.0% | |
| ! | ACCACCAAAGCTAATTTTGA+TGG | + | chr4.2:98896-98915 | None:intergenic | 35.0% |
| !! | ACCATCAAAATTAGCTTTGG+TGG | - | chr4.2:98892-98911 | MS.gene93738:intron | 35.0% |
| !! | CAGTTTGCTAATTTGAGCAA+CGG | + | chr4.2:98446-98465 | None:intergenic | 35.0% |
| !! | GACTTTTGGTTGTTTTCTCT+GGG | - | chr4.2:98942-98961 | MS.gene93738:CDS | 35.0% |
| !! | TGACTTTTGGTTGTTTTCTC+TGG | - | chr4.2:98941-98960 | MS.gene93738:CDS | 35.0% |
| ATTGGAGACTGAAACTGAAG+AGG | + | chr4.2:97928-97947 | None:intergenic | 40.0% | |
| CCGGTTGTAATTGAAGAAGA+CGG | + | chr4.2:97446-97465 | None:intergenic | 40.0% | |
| CCGTCTTCTTCAATTACAAC+CGG | - | chr4.2:97443-97462 | MS.gene93738:CDS | 40.0% | |
| CGCAACAATAAGATCTAAGG+AGG | - | chr4.2:98774-98793 | MS.gene93738:intron | 40.0% | |
| CTTCAGTTTCAGTCTCCAAT+CGG | - | chr4.2:97928-97947 | MS.gene93738:CDS | 40.0% | |
| GCAATCTTATGAGAAGAGTC+TGG | + | chr4.2:97904-97923 | None:intergenic | 40.0% | |
| GGAAGAGATCTTGTACACAT+TGG | - | chr4.2:98234-98253 | MS.gene93738:intron | 40.0% | |
| GGGGGCAATTGATTATTCAA+AGG | - | chr4.2:98864-98883 | MS.gene93738:intron | 40.0% | |
| GTGCTTCTCAATAATGCTCA+CGG | + | chr4.2:98800-98819 | None:intergenic | 40.0% | |
| GTTGTAATTCCAGCAACTAG+TGG | + | chr4.2:97605-97624 | None:intergenic | 40.0% | |
| GTTGTAATTGAAGAAGACGG+CGG | + | chr4.2:97443-97462 | None:intergenic | 40.0% | |
| TAGAAGGAGGAAAGTGAAGA+AGG | + | chr4.2:97478-97497 | None:intergenic | 40.0% | |
| TATGCAGCATCAGTGATGTA+TGG | - | chr4.2:98481-98500 | MS.gene93738:CDS | 40.0% | |
| TATTGTTGCGTATCTCTGGA+GGG | + | chr4.2:98764-98783 | None:intergenic | 40.0% | |
| TGAGAAAGAAACTGACCGAT+TGG | + | chr4.2:97946-97965 | None:intergenic | 40.0% | |
| TGAGATGATCCAAAACCACT+TGG | - | chr4.2:98384-98403 | MS.gene93738:CDS | 40.0% | |
| TGATGAATACTACTGTCGTC+TGG | + | chr4.2:98574-98593 | None:intergenic | 40.0% | |
| TTATTGTTGCGTATCTCTGG+AGG | + | chr4.2:98765-98784 | None:intergenic | 40.0% | |
| TTTGCTAATTTGAGCAACGG+AGG | + | chr4.2:98443-98462 | None:intergenic | 40.0% | |
| ! | CATTATTGAGAAGCACACAG+AGG | - | chr4.2:98804-98823 | MS.gene93738:intron | 40.0% |
| ! | CTCATCCTGAAGTATCTACT+TGG | - | chr4.2:98650-98669 | MS.gene93738:intron | 40.0% |
| ! | TTCAGGATGAGATGTCACTT+GGG | + | chr4.2:98641-98660 | None:intergenic | 40.0% |
| ! | TTCTTAAGCGAGTTGACCAA+AGG | - | chr4.2:98509-98528 | MS.gene93738:CDS | 40.0% |
| !!! | CTTTTTCAGGAGAATTGCTG+AGG | - | chr4.2:98183-98202 | MS.gene93738:CDS | 40.0% |
| !!! | GAGAAGGATCTTGGTTTTGT+TGG | + | chr4.2:98056-98075 | None:intergenic | 40.0% |
| !!! | GTTTTAGAGGCTGTGACTTT+TGG | - | chr4.2:98928-98947 | MS.gene93738:CDS | 40.0% |
| AAATGAAAGGAGGACAGCTC+TGG | - | chr4.2:98213-98232 | MS.gene93738:intron | 45.0% | |
| AAGCATGAGAGACTCTCTGT+TGG | - | chr4.2:97793-97812 | MS.gene93738:CDS | 45.0% | |
| AAGGAGGAAAGTGAAGAAGG+CGG | + | chr4.2:97475-97494 | None:intergenic | 45.0% | |
| AATGGTTATCACACGTGAGG+GGG | - | chr4.2:98846-98865 | MS.gene93738:intron | 45.0% | |
| ACATAGTTACGCAACCTGGT+CGG | + | chr4.2:98721-98740 | None:intergenic | 45.0% | |
| AGCATGAGAGACTCTCTGTT+GGG | - | chr4.2:97794-97813 | MS.gene93738:CDS | 45.0% | |
| AGGTCAAGGCAAATGAAAGG+AGG | - | chr4.2:98203-98222 | MS.gene93738:CDS | 45.0% | |
| AGTCTCTCATGCTTATGCCT+AGG | + | chr4.2:97787-97806 | None:intergenic | 45.0% | |
| ATACCATAGCCAAATCCACC+AGG | + | chr4.2:98694-98713 | None:intergenic | 45.0% | |
| ATGTGGTAGGAGAAGGATCT+TGG | + | chr4.2:98065-98084 | None:intergenic | 45.0% | |
| ATTGTGGATGATGCAAGACC+TGG | - | chr4.2:98598-98617 | MS.gene93738:CDS | 45.0% | |
| ATTGTTTGGAAGACCCGGAA+TGG | - | chr4.2:98828-98847 | MS.gene93738:intron | 45.0% | |
| CAAAACAAGATCGGAGGATG+TGG | + | chr4.2:98082-98101 | None:intergenic | 45.0% | |
| CATCACATAGTTACGCAACC+TGG | + | chr4.2:98725-98744 | None:intergenic | 45.0% | |
| CCTGTACAAAACAAGATCGG+AGG | + | chr4.2:98088-98107 | None:intergenic | 45.0% | |
| CTCACGTGTGATAACCATTC+CGG | + | chr4.2:98845-98864 | None:intergenic | 45.0% | |
| CTGAGGTCAAGGCAAATGAA+AGG | - | chr4.2:98200-98219 | MS.gene93738:CDS | 45.0% | |
| CTTCAGGATGAGATGTCACT+TGG | + | chr4.2:98642-98661 | None:intergenic | 45.0% | |
| GAATGGTTATCACACGTGAG+GGG | - | chr4.2:98845-98864 | MS.gene93738:intron | 45.0% | |
| GAGCAACATCACGATCAGAT+TGG | + | chr4.2:98029-98048 | None:intergenic | 45.0% | |
| GGAATGGTTATCACACGTGA+GGG | - | chr4.2:98844-98863 | MS.gene93738:intron | 45.0% | |
| GTCTCTCATGCTTATGCCTA+GGG | + | chr4.2:97786-97805 | None:intergenic | 45.0% | |
| TACCAAGAATGAGAGCCAAG+TGG | + | chr4.2:98402-98421 | None:intergenic | 45.0% | |
| TCACGTGTGATAACCATTCC+GGG | + | chr4.2:98844-98863 | None:intergenic | 45.0% | |
| TCATGCTTATGCCTAGGGAT+GGG | + | chr4.2:97781-97800 | None:intergenic | 45.0% | |
| TCTATAACCCCAGATGCAAC+TGG | - | chr4.2:98298-98317 | MS.gene93738:intron | 45.0% | |
| TGATCCATTCCTTGGTTGGT+AGG | - | chr4.2:97741-97760 | MS.gene93738:CDS | 45.0% | |
| TTATGAGAAGAGTCTGGGTC+TGG | + | chr4.2:97898-97917 | None:intergenic | 45.0% | |
| ! | AACCACTTGGCTCTCATTCT+TGG | - | chr4.2:98397-98416 | MS.gene93738:CDS | 45.0% |
| ! | AGTTGCATCTGGGGTTATAG+AGG | + | chr4.2:98299-98318 | None:intergenic | 45.0% |
| ! | CTCATTCTTGGTAACAGAGC+AGG | - | chr4.2:98409-98428 | MS.gene93738:CDS | 45.0% |
| ! | GAATGAGAGCCAAGTGGTTT+TGG | + | chr4.2:98396-98415 | None:intergenic | 45.0% |
| ! | GTTGCATCTGGGGTTATAGA+GGG | + | chr4.2:98298-98317 | None:intergenic | 45.0% |
| ! | TGATTGTTGTTGCTTGCTGC+TGG | - | chr4.2:97548-97567 | MS.gene93738:CDS | 45.0% |
| !! | AAGCACACAGAGGCATTGTT+TGG | - | chr4.2:98814-98833 | MS.gene93738:intron | 45.0% |
| !!! | CCTCCGATCTTGTTTTGTAC+AGG | - | chr4.2:98085-98104 | MS.gene93738:CDS | 45.0% |
| AACCCCAGATGCAACTGGTA+GGG | - | chr4.2:98303-98322 | MS.gene93738:intron | 50.0% | |
| AAGAAGACGGCGGTGATAAG+AGG | + | chr4.2:97433-97452 | None:intergenic | 50.0% | |
| AAGTGCACACCTACCAACCA+AGG | + | chr4.2:97753-97772 | None:intergenic | 50.0% | |
| ACAAGATCGGAGGATGTGGT+AGG | + | chr4.2:98078-98097 | None:intergenic | 50.0% | |
| ACTTCTCCAGCTGGAACCTT+TGG | + | chr4.2:98528-98547 | None:intergenic | 50.0% | |
| AGTTGACCAAAGGTTCCAGC+TGG | - | chr4.2:98519-98538 | MS.gene93738:CDS | 50.0% | |
| ATTGGAGCTGGAGAAGTTGC+TGG | + | chr4.2:98011-98030 | None:intergenic | 50.0% | |
| CACACCTACCAACCAAGGAA+TGG | + | chr4.2:97748-97767 | None:intergenic | 50.0% | |
| CAGGAGAATTGCTGAGGTCA+AGG | - | chr4.2:98189-98208 | MS.gene93738:CDS | 50.0% | |
| CATCACGATCAGATTGGAGC+TGG | + | chr4.2:98023-98042 | None:intergenic | 50.0% | |
| CATGCTTATGCCTAGGGATG+GGG | + | chr4.2:97780-97799 | None:intergenic | 50.0% | |
| CCAGGCCAAGTAGATACTTC+AGG | + | chr4.2:98658-98677 | None:intergenic | 50.0% | |
| CGGAATGGTTATCACACGTG+AGG | - | chr4.2:98843-98862 | MS.gene93738:intron | 50.0% | |
| CTCATGCTTATGCCTAGGGA+TGG | + | chr4.2:97782-97801 | None:intergenic | 50.0% | |
| GCTATGGTATAAAGCCGACC+AGG | - | chr4.2:98704-98723 | MS.gene93738:intron | 50.0% | |
| TAACCCCAGATGCAACTGGT+AGG | - | chr4.2:98302-98321 | MS.gene93738:intron | 50.0% | |
| TAGCAAACTGAGGATAGGGC+AGG | - | chr4.2:98456-98475 | MS.gene93738:CDS | 50.0% | |
| ! | CAACTGGTAGGGTCGATTCA+TGG | - | chr4.2:98314-98333 | MS.gene93738:CDS | 50.0% |
| ! | CCTGAAGTATCTACTTGGCC+TGG | - | chr4.2:98655-98674 | MS.gene93738:intron | 50.0% |
| ! | GAAGTATCTACTTGGCCTGG+TGG | - | chr4.2:98658-98677 | MS.gene93738:intron | 50.0% |
| ! | GAAGTGTCCACTTCACTACC+AGG | + | chr4.2:98619-98638 | None:intergenic | 50.0% |
| ! | TGCAAGACCTGGTAGTGAAG+TGG | - | chr4.2:98609-98628 | MS.gene93738:CDS | 50.0% |
| !! | AGTCCTGGTGGATTTGGCTA+TGG | - | chr4.2:98688-98707 | MS.gene93738:intron | 50.0% |
| !! | CTTTCATCGACTTCTCCAGC+TGG | + | chr4.2:98537-98556 | None:intergenic | 50.0% |
| !! | GAGGCATTGTTTGGAAGACC+CGG | - | chr4.2:98823-98842 | MS.gene93738:intron | 50.0% |
| !! | GATGTCAGTCCTGGTGGATT+TGG | - | chr4.2:98682-98701 | MS.gene93738:intron | 50.0% |
| AAGACGGCGGTGATAAGAGG+AGG | + | chr4.2:97430-97449 | None:intergenic | 55.0% | |
| AGGAAAGTGAAGAAGGCGGC+AGG | + | chr4.2:97471-97490 | None:intergenic | 55.0% | |
| CGACCCTACCAGTTGCATCT+GGG | + | chr4.2:98309-98328 | None:intergenic | 55.0% | |
| CTGGGATGTTGAGAGCTACG+TGG | - | chr4.2:98960-98979 | MS.gene93738:CDS | 55.0% | |
| CTTATGCCTAGGGATGGGGA+CGG | + | chr4.2:97776-97795 | None:intergenic | 55.0% | |
| GACCCTACCAGTTGCATCTG+GGG | + | chr4.2:98308-98327 | None:intergenic | 55.0% | |
| TCGACCCTACCAGTTGCATC+TGG | + | chr4.2:98310-98329 | None:intergenic | 55.0% | |
| TCGGAGGATGTGGTAGGAGA+AGG | + | chr4.2:98072-98091 | None:intergenic | 55.0% | |
| TGATAAGAGGAGGACGGCTG+TGG | + | chr4.2:97420-97439 | None:intergenic | 55.0% | |
| TTGAGAGCTACGTGGACTCG+AGG | - | chr4.2:98968-98987 | MS.gene93738:CDS | 55.0% | |
| !! | CTGGAGGGTATCACCGTCAA+AGG | + | chr4.2:98749-98768 | None:intergenic | 55.0% |
| ACCGCAGCAGGCACAAGATG+GGG | + | chr4.2:97985-98004 | None:intergenic | 60.0% | |
| AGAAGAGTCTGGGTCTGGCG+AGG | + | chr4.2:97893-97912 | None:intergenic | 60.0% | |
| CACCGCAGCAGGCACAAGAT+GGG | + | chr4.2:97986-98005 | None:intergenic | 60.0% | |
| CGGCGGTGATAAGAGGAGGA+CGG | + | chr4.2:97426-97445 | None:intergenic | 60.0% | |
| GTGAAGAAGGCGGCAGGATC+CGG | + | chr4.2:97465-97484 | None:intergenic | 60.0% | |
| AGAGTCTGGGTCTGGCGAGG+AGG | + | chr4.2:97890-97909 | None:intergenic | 65.0% | |
| AGTTGCTGGTCCACCGCAGC+AGG | + | chr4.2:97997-98016 | None:intergenic | 65.0% | |
| CCACCGCAGCAGGCACAAGA+TGG | + | chr4.2:97987-98006 | None:intergenic | 65.0% | |
| CCAGGACTGACATCGCCACC+AGG | + | chr4.2:98676-98695 | None:intergenic | 65.0% | |
| CCATCTTGTGCCTGCTGCGG+TGG | - | chr4.2:97984-98003 | MS.gene93738:CDS | 65.0% | |
| CCCCCATCTTGTGCCTGCTG+CGG | - | chr4.2:97981-98000 | MS.gene93738:CDS | 65.0% | |
| CCGCAGCAGGCACAAGATGG+GGG | + | chr4.2:97984-98003 | None:intergenic | 65.0% | |
| GCACTTCCGTCCCCATCCCT+AGG | - | chr4.2:97767-97786 | MS.gene93738:CDS | 65.0% | |
| GGTGGCGATGTCAGTCCTGG+TGG | - | chr4.2:98676-98695 | MS.gene93738:intron | 65.0% | |
| TGGGTCTGGCGAGGAGGAAG+AGG | + | chr4.2:97884-97903 | None:intergenic | 65.0% | |
| ! | CCTGGTGGCGATGTCAGTCC+TGG | - | chr4.2:98673-98692 | MS.gene93738:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 97404 | 99011 | 97404 | ID=MS.gene93738 |
| chr4.2 | mRNA | 97404 | 99011 | 97404 | ID=MS.gene93738.t1;Parent=MS.gene93738 |
| chr4.2 | exon | 98910 | 99011 | 98910 | ID=MS.gene93738.t1.exon1;Parent=MS.gene93738.t1 |
| chr4.2 | CDS | 98910 | 99011 | 98910 | ID=cds.MS.gene93738.t1;Parent=MS.gene93738.t1 |
| chr4.2 | exon | 98309 | 98652 | 98309 | ID=MS.gene93738.t1.exon2;Parent=MS.gene93738.t1 |
| chr4.2 | CDS | 98309 | 98652 | 98309 | ID=cds.MS.gene93738.t1;Parent=MS.gene93738.t1 |
| chr4.2 | exon | 97404 | 98223 | 97404 | ID=MS.gene93738.t1.exon3;Parent=MS.gene93738.t1 |
| chr4.2 | CDS | 97404 | 98223 | 97404 | ID=cds.MS.gene93738.t1;Parent=MS.gene93738.t1 |
| Gene Sequence |
| Protein sequence |
