Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053182.t1 | KEH28565.1 | 82.9 | 129 | 21 | 1 | 1 | 129 | 1 | 128 | 1.80E-38 | 168.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053182.t1 | A0A072URR4 | 82.9 | 129 | 21 | 1 | 1 | 129 | 1 | 128 | 1.3e-38 | 168.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053182 | MS.gene29794 | 0.806134 | 9.53E-50 | -1.69E-46 |
| MS.gene053182 | MS.gene57704 | 0.806714 | 7.19E-50 | -1.69E-46 |
| MS.gene053182 | MS.gene58768 | 0.811686 | 6.18E-51 | -1.69E-46 |
| MS.gene053182 | MS.gene58769 | 0.810367 | 1.19E-50 | -1.69E-46 |
| MS.gene053182 | MS.gene58793 | 0.812689 | 3.74E-51 | -1.69E-46 |
| MS.gene053182 | MS.gene70277 | 0.804142 | 2.49E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053182.t1 | MTR_4g007570 | 84.211 | 133 | 15 | 3 | 1 | 129 | 1 | 131 | 1.29e-53 | 165 |
| MS.gene053182.t1 | MTR_4g007570 | 65.563 | 151 | 28 | 4 | 1 | 129 | 1 | 149 | 6.91e-46 | 146 |
| MS.gene053182.t1 | MTR_4g007580 | 87.597 | 129 | 15 | 1 | 1 | 129 | 1 | 128 | 1.73e-45 | 144 |
| MS.gene053182.t1 | MTR_4g007560 | 46.584 | 161 | 51 | 7 | 1 | 129 | 1 | 158 | 6.05e-18 | 75.1 |
| MS.gene053182.t1 | MTR_4g007590 | 38.926 | 149 | 64 | 6 | 1 | 129 | 1 | 142 | 4.27e-17 | 72.4 |
| MS.gene053182.t1 | MTR_4g007620 | 50.000 | 78 | 29 | 3 | 1 | 69 | 13 | 89 | 8.26e-12 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACAAGCACATGAACTTAA+TGG | 0.248141 | 4.2:+92225864 | MS.gene053182:CDS |
| GCACATGAACTTAATGGAAA+TGG | 0.373587 | 4.2:+92225870 | MS.gene053182:CDS |
| ATAAGAAAAGGAGGAGGAAT+AGG | 0.376818 | 4.2:+92225975 | MS.gene053182:CDS |
| ATTATCGGTGCTGGAGTTAT+CGG | 0.395260 | 4.2:+92226029 | MS.gene053182:CDS |
| TAATGATGATGCAGAACTAT+TGG | 0.399049 | 4.2:-92226084 | None:intergenic |
| GGAGGAATAGGCAGAGGAAT+TGG | 0.409519 | 4.2:+92225987 | MS.gene053182:CDS |
| GGAGCGGGTGTTGGAATTAT+CGG | 0.416578 | 4.2:+92226014 | MS.gene053182:CDS |
| AGAGGAATTGGCAGAGGAGC+GGG | 0.433946 | 4.2:+92225999 | MS.gene053182:CDS |
| ATCTTGTTCATTTGATATGT+TGG | 0.442923 | 4.2:-92225832 | None:intergenic |
| ATTGGCAGAGGAGCGGGTGT+TGG | 0.494874 | 4.2:+92226005 | MS.gene053182:CDS |
| CTATCTGTTACCATAAGAAA+AGG | 0.494904 | 4.2:+92225963 | MS.gene053182:CDS |
| GGTGTTGGAATTATCGGTGC+TGG | 0.499018 | 4.2:+92226020 | MS.gene053182:CDS |
| GGAGTGATGACTCCTGTCAT+AGG | 0.507291 | 4.2:-92226063 | None:intergenic |
| GAACCACAGAGTTGCGATGA+TGG | 0.507304 | 4.2:+92225917 | MS.gene053182:CDS |
| AATGTTGAGACACAAACATA+AGG | 0.523934 | 4.2:-92226116 | None:intergenic |
| GCGGGTCATCCGCCTATGAC+AGG | 0.527211 | 4.2:+92226051 | MS.gene053182:CDS |
| AAAGGAGGAGGAATAGGCAG+AGG | 0.534351 | 4.2:+92225981 | MS.gene053182:CDS |
| GAGCTCTTGAAATGATCAGA+AGG | 0.576553 | 4.2:-92225806 | None:intergenic |
| ATAGGCAGAGGAATTGGCAG+AGG | 0.585765 | 4.2:+92225993 | MS.gene053182:CDS |
| ATCGGTGCTGGAGTTATCGG+CGG | 0.587257 | 4.2:+92226032 | MS.gene053182:CDS |
| CAGAGGAATTGGCAGAGGAG+CGG | 0.605437 | 4.2:+92225998 | MS.gene053182:CDS |
| TCTGTTACCATAAGAAAAGG+AGG | 0.631131 | 4.2:+92225966 | MS.gene053182:CDS |
| ACTTAATGGAAATGGAAACA+TGG | 0.633060 | 4.2:+92225878 | MS.gene053182:CDS |
| TCGGTGCTGGAGTTATCGGC+GGG | 0.638526 | 4.2:+92226033 | MS.gene053182:CDS |
| TTTCCATCATCGCAACTCTG+TGG | 0.639342 | 4.2:-92225920 | None:intergenic |
| GTTACCATAAGAAAAGGAGG+AGG | 0.643572 | 4.2:+92225969 | MS.gene053182:CDS |
| GTGATGACTCCTGTCATAGG+CGG | 0.711939 | 4.2:-92226060 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATTTTATGTTTGAGCTTT+TGG | + | chr4.2:92226138-92226157 | MS.gene053182:CDS | 20.0% |
| ! | ATCTTGTTCATTTGATATGT+TGG | - | chr4.2:92225835-92225854 | None:intergenic | 25.0% |
| AAACAAGCACATGAACTTAA+TGG | + | chr4.2:92225864-92225883 | MS.gene053182:CDS | 30.0% | |
| AATGTTGAGACACAAACATA+AGG | - | chr4.2:92226119-92226138 | None:intergenic | 30.0% | |
| ACTTAATGGAAATGGAAACA+TGG | + | chr4.2:92225878-92225897 | MS.gene053182:CDS | 30.0% | |
| CTATCTGTTACCATAAGAAA+AGG | + | chr4.2:92225963-92225982 | MS.gene053182:CDS | 30.0% | |
| ! | TAATGATGATGCAGAACTAT+TGG | - | chr4.2:92226087-92226106 | None:intergenic | 30.0% |
| ATAAGAAAAGGAGGAGGAAT+AGG | + | chr4.2:92225975-92225994 | MS.gene053182:CDS | 35.0% | |
| GCACATGAACTTAATGGAAA+TGG | + | chr4.2:92225870-92225889 | MS.gene053182:CDS | 35.0% | |
| TCTGTTACCATAAGAAAAGG+AGG | + | chr4.2:92225966-92225985 | MS.gene053182:CDS | 35.0% | |
| ! | TATTCCTCCTCCTTTTCTTA+TGG | - | chr4.2:92225976-92225995 | None:intergenic | 35.0% |
| GAGCTCTTGAAATGATCAGA+AGG | - | chr4.2:92225809-92225828 | None:intergenic | 40.0% | |
| GTTACCATAAGAAAAGGAGG+AGG | + | chr4.2:92225969-92225988 | MS.gene053182:CDS | 40.0% | |
| !! | ATTATCGGTGCTGGAGTTAT+CGG | + | chr4.2:92226029-92226048 | MS.gene053182:CDS | 40.0% |
| TTTCCATCATCGCAACTCTG+TGG | - | chr4.2:92225923-92225942 | None:intergenic | 45.0% | |
| AAAGGAGGAGGAATAGGCAG+AGG | + | chr4.2:92225981-92226000 | MS.gene053182:CDS | 50.0% | |
| ATAGGCAGAGGAATTGGCAG+AGG | + | chr4.2:92225993-92226012 | MS.gene053182:CDS | 50.0% | |
| GAACCACAGAGTTGCGATGA+TGG | + | chr4.2:92225917-92225936 | MS.gene053182:CDS | 50.0% | |
| GGAGGAATAGGCAGAGGAAT+TGG | + | chr4.2:92225987-92226006 | MS.gene053182:CDS | 50.0% | |
| GGAGTGATGACTCCTGTCAT+AGG | - | chr4.2:92226066-92226085 | None:intergenic | 50.0% | |
| GTGATGACTCCTGTCATAGG+CGG | - | chr4.2:92226063-92226082 | None:intergenic | 50.0% | |
| ! | GGTGTTGGAATTATCGGTGC+TGG | + | chr4.2:92226020-92226039 | MS.gene053182:CDS | 50.0% |
| !! | GGAGCGGGTGTTGGAATTAT+CGG | + | chr4.2:92226014-92226033 | MS.gene053182:CDS | 50.0% |
| AGAGGAATTGGCAGAGGAGC+GGG | + | chr4.2:92225999-92226018 | MS.gene053182:CDS | 55.0% | |
| CAGAGGAATTGGCAGAGGAG+CGG | + | chr4.2:92225998-92226017 | MS.gene053182:CDS | 55.0% | |
| !! | ATCGGTGCTGGAGTTATCGG+CGG | + | chr4.2:92226032-92226051 | MS.gene053182:CDS | 55.0% |
| ATTGGCAGAGGAGCGGGTGT+TGG | + | chr4.2:92226005-92226024 | MS.gene053182:CDS | 60.0% | |
| !! | TCGGTGCTGGAGTTATCGGC+GGG | + | chr4.2:92226033-92226052 | MS.gene053182:CDS | 60.0% |
| GCGGGTCATCCGCCTATGAC+AGG | + | chr4.2:92226051-92226070 | MS.gene053182:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 92225777 | 92226166 | 92225777 | ID=MS.gene053182 |
| chr4.2 | mRNA | 92225777 | 92226166 | 92225777 | ID=MS.gene053182.t1;Parent=MS.gene053182 |
| chr4.2 | exon | 92225777 | 92226166 | 92225777 | ID=MS.gene053182.t1.exon1;Parent=MS.gene053182.t1 |
| chr4.2 | CDS | 92225777 | 92226166 | 92225777 | ID=cds.MS.gene053182.t1;Parent=MS.gene053182.t1 |
| Gene Sequence |
| Protein sequence |
