Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58793.t1 | GAU11971.1 | 83.9 | 149 | 18 | 4 | 1 | 146 | 1 | 146 | 4.30E-65 | 257.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58793.t1 | A0A392P6I4 | 84.6 | 149 | 17 | 4 | 1 | 146 | 1 | 146 | 1.2e-64 | 255.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04998 | MS.gene58793 | 0.899305 | 2.26E-77 | -1.69E-46 |
| MS.gene051226 | MS.gene58793 | 0.874203 | 8.11E-68 | -1.69E-46 |
| MS.gene051837 | MS.gene58793 | 0.822512 | 2.28E-53 | -1.69E-46 |
| MS.gene052296 | MS.gene58793 | 0.821081 | 4.88E-53 | -1.69E-46 |
| MS.gene052878 | MS.gene58793 | 0.807239 | 5.57E-50 | -1.69E-46 |
| MS.gene053182 | MS.gene58793 | 0.812689 | 3.74E-51 | -1.69E-46 |
| MS.gene053879 | MS.gene58793 | 0.810631 | 1.05E-50 | -1.69E-46 |
| MS.gene054892 | MS.gene58793 | 0.838559 | 2.68E-57 | -1.69E-46 |
| MS.gene054893 | MS.gene58793 | 0.834094 | 3.66E-56 | -1.69E-46 |
| MS.gene056992 | MS.gene58793 | 0.813023 | 3.16E-51 | -1.69E-46 |
| MS.gene057093 | MS.gene58793 | 0.888709 | 4.64E-73 | -1.69E-46 |
| MS.gene057167 | MS.gene58793 | 0.840901 | 6.58E-58 | -1.69E-46 |
| MS.gene057432 | MS.gene58793 | 0.81845 | 1.95E-52 | -1.69E-46 |
| MS.gene057622 | MS.gene58793 | 0.854651 | 1.07E-61 | -1.69E-46 |
| MS.gene057999 | MS.gene58793 | 0.809106 | 2.23E-50 | -1.69E-46 |
| MS.gene058592 | MS.gene58793 | 0.821194 | 4.60E-53 | -1.69E-46 |
| MS.gene06023 | MS.gene58793 | 0.8436 | 1.27E-58 | -1.69E-46 |
| MS.gene060259 | MS.gene58793 | 0.818045 | 2.41E-52 | -1.69E-46 |
| MS.gene060446 | MS.gene58793 | 0.821324 | 4.29E-53 | -1.69E-46 |
| MS.gene060964 | MS.gene58793 | 0.839525 | 1.50E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58793.t1 | MTR_4g116530 | 93.289 | 149 | 7 | 2 | 1 | 146 | 1 | 149 | 3.19e-100 | 284 |
| MS.gene58793.t1 | MTR_2g461190 | 59.459 | 148 | 49 | 6 | 1 | 145 | 1 | 140 | 8.60e-48 | 151 |
| MS.gene58793.t1 | MTR_4g106650 | 39.130 | 69 | 42 | 0 | 66 | 134 | 62 | 130 | 5.15e-12 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58793.t1 | AT5G25240 | 39.024 | 123 | 56 | 6 | 17 | 127 | 11 | 126 | 1.21e-20 | 82.0 |
| MS.gene58793.t1 | AT5G14895 | 38.202 | 89 | 42 | 3 | 61 | 136 | 60 | 148 | 6.44e-12 | 60.5 |
Find 39 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTAAGTGGAAAACGTTTCTT+AGG | 0.293276 | 8.1:-10485845 | MS.gene58793:CDS |
| CTTAGGAAAATTAGTGGGTA+TGG | 0.339302 | 8.1:-10485828 | MS.gene58793:CDS |
| TATGCTCTTAATTTCAATAG+TGG | 0.374345 | 8.1:-10485765 | MS.gene58793:CDS |
| TCATTCTGGCGACGTGCATC+TGG | 0.387586 | 8.1:+10485678 | None:intergenic |
| ATATATATCATGTTTCATTC+TGG | 0.388657 | 8.1:+10485664 | None:intergenic |
| AAACAACATGGCTGCCTATG+AGG | 0.423463 | 8.1:-10486096 | None:intergenic |
| CACCAATGGTCTTCCCTCAT+AGG | 0.431252 | 8.1:+10486082 | None:intergenic |
| ATGCTCTTAATTTCAATAGT+GGG | 0.436223 | 8.1:-10485764 | MS.gene58793:CDS |
| AACGGGTATGGATGCGGGTG+CGG | 0.443003 | 8.1:-10486023 | MS.gene58793:CDS |
| AAGACCATTGGTGATGGAAA+TGG | 0.450625 | 8.1:-10486072 | MS.gene58793:CDS |
| GAAAATAGTGAAGGAAATAG+AGG | 0.458494 | 8.1:-10485933 | MS.gene58793:CDS |
| TGGCGACGTGCATCTGGAAA+AGG | 0.467598 | 8.1:+10485684 | None:intergenic |
| CTTTCCATTTCCATCACCAA+TGG | 0.472269 | 8.1:+10486068 | None:intergenic |
| TTAGGAAAATTAGTGGGTAT+GGG | 0.477370 | 8.1:-10485827 | MS.gene58793:CDS |
| GTGGCGTTGCCACGATTTCA+AGG | 0.486009 | 8.1:+10485983 | None:intergenic |
| TTCGAAGAGACCAACGGGTA+TGG | 0.486097 | 8.1:-10486035 | MS.gene58793:CDS |
| GAGACTTCAGAAGTGATTGC+TGG | 0.496587 | 8.1:-10485870 | MS.gene58793:CDS |
| GGTATGGATGCGGGTGCGGA+TGG | 0.500992 | 8.1:-10486019 | MS.gene58793:CDS |
| AAGATTTCGAAGAGACCAAC+GGG | 0.512584 | 8.1:-10486040 | MS.gene58793:CDS |
| GAGACCAACGGGTATGGATG+CGG | 0.514745 | 8.1:-10486029 | MS.gene58793:CDS |
| AGACCAACGGGTATGGATGC+GGG | 0.516345 | 8.1:-10486028 | MS.gene58793:CDS |
| AGGAGAAGAGACATGGATGA+TGG | 0.526434 | 8.1:-10485913 | MS.gene58793:CDS |
| TGCTCTTAATTTCAATAGTG+GGG | 0.526773 | 8.1:-10485763 | MS.gene58793:CDS |
| ACGTTTCTTAGGAAAATTAG+TGG | 0.531357 | 8.1:-10485834 | MS.gene58793:CDS |
| GGACAAGTTGAGGAAGATGA+AGG | 0.537471 | 8.1:-10485892 | MS.gene58793:CDS |
| AACAACATGGCTGCCTATGA+GGG | 0.543567 | 8.1:-10486095 | None:intergenic |
| TGGCAACGCCACGACGAAGA+AGG | 0.555768 | 8.1:-10485972 | MS.gene58793:CDS |
| GAAATAGAGGAGAAGAGACA+TGG | 0.562044 | 8.1:-10485920 | MS.gene58793:CDS |
| AAGTGATTGCTGGTCCTAAG+TGG | 0.571200 | 8.1:-10485860 | MS.gene58793:CDS |
| AAATATTCACCTTGAAATCG+TGG | 0.578098 | 8.1:-10485992 | MS.gene58793:CDS |
| CATGGATGATGGACAAGTTG+AGG | 0.582027 | 8.1:-10485902 | MS.gene58793:CDS |
| CGTTTCTTAGGAAAATTAGT+GGG | 0.584307 | 8.1:-10485833 | MS.gene58793:CDS |
| TGAGGGAAGACCATTGGTGA+TGG | 0.585967 | 8.1:-10486078 | MS.gene58793:CDS |
| CTACTTAATGAAAATAGTGA+AGG | 0.588080 | 8.1:-10485942 | MS.gene58793:CDS |
| TGCCTATGAGGGAAGACCAT+TGG | 0.600498 | 8.1:-10486084 | MS.gene58793:CDS |
| CAAGATTTCGAAGAGACCAA+CGG | 0.625236 | 8.1:-10486041 | MS.gene58793:CDS |
| GATGCTTACCTTCTTCGTCG+TGG | 0.640185 | 8.1:+10485964 | None:intergenic |
| AAAAGGATTAGAAAACCTTG+TGG | 0.651946 | 8.1:+10485701 | None:intergenic |
| GCACCCGCATCCATACCCGT+TGG | 0.669123 | 8.1:+10486025 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGCTCTTAATTTCAATAGT+GGG | - | chr8.1:10485996-10486015 | MS.gene58793:CDS | 25.0% |
| ! | CTACTTAATGAAAATAGTGA+AGG | - | chr8.1:10485818-10485837 | MS.gene58793:CDS | 25.0% |
| ! | TATGCTCTTAATTTCAATAG+TGG | - | chr8.1:10485995-10486014 | MS.gene58793:CDS | 25.0% |
| AAAAGGATTAGAAAACCTTG+TGG | + | chr8.1:10486062-10486081 | None:intergenic | 30.0% | |
| AAATATTCACCTTGAAATCG+TGG | - | chr8.1:10485768-10485787 | MS.gene58793:CDS | 30.0% | |
| ACGTTTCTTAGGAAAATTAG+TGG | - | chr8.1:10485926-10485945 | MS.gene58793:CDS | 30.0% | |
| CGTTTCTTAGGAAAATTAGT+GGG | - | chr8.1:10485927-10485946 | MS.gene58793:CDS | 30.0% | |
| GAAAATAGTGAAGGAAATAG+AGG | - | chr8.1:10485827-10485846 | MS.gene58793:CDS | 30.0% | |
| TGCTCTTAATTTCAATAGTG+GGG | - | chr8.1:10485997-10486016 | MS.gene58793:CDS | 30.0% | |
| ! | ATGGGAAAAAACAAAAGCAT+AGG | - | chr8.1:10485951-10485970 | MS.gene58793:CDS | 30.0% |
| ! | TTAGGAAAATTAGTGGGTAT+GGG | - | chr8.1:10485933-10485952 | MS.gene58793:CDS | 30.0% |
| CTAAGTGGAAAACGTTTCTT+AGG | - | chr8.1:10485915-10485934 | MS.gene58793:CDS | 35.0% | |
| GAAAACCTTGTGGAAAAACT+TGG | + | chr8.1:10486052-10486071 | None:intergenic | 35.0% | |
| ! | CTAAGAAACGTTTTCCACTT+AGG | + | chr8.1:10485917-10485936 | None:intergenic | 35.0% |
| ! | CTTAGGAAAATTAGTGGGTA+TGG | - | chr8.1:10485932-10485951 | MS.gene58793:CDS | 35.0% |
| AACCTTGTGGAAAAACTTGG+AGG | + | chr8.1:10486049-10486068 | None:intergenic | 40.0% | |
| AAGATTTCGAAGAGACCAAC+GGG | - | chr8.1:10485720-10485739 | MS.gene58793:CDS | 40.0% | |
| CAAGATTTCGAAGAGACCAA+CGG | - | chr8.1:10485719-10485738 | MS.gene58793:CDS | 40.0% | |
| CTTTCCATTTCCATCACCAA+TGG | + | chr8.1:10485695-10485714 | None:intergenic | 40.0% | |
| GAAATAGAGGAGAAGAGACA+TGG | - | chr8.1:10485840-10485859 | MS.gene58793:CDS | 40.0% | |
| !! | AAGACCATTGGTGATGGAAA+TGG | - | chr8.1:10485688-10485707 | MS.gene58793:CDS | 40.0% |
| AAGTGATTGCTGGTCCTAAG+TGG | - | chr8.1:10485900-10485919 | MS.gene58793:CDS | 45.0% | |
| AGGAGAAGAGACATGGATGA+TGG | - | chr8.1:10485847-10485866 | MS.gene58793:CDS | 45.0% | |
| GAGACTTCAGAAGTGATTGC+TGG | - | chr8.1:10485890-10485909 | MS.gene58793:CDS | 45.0% | |
| ! | GGACAAGTTGAGGAAGATGA+AGG | - | chr8.1:10485868-10485887 | MS.gene58793:CDS | 45.0% |
| ! | TGCCTCCAAGTTTTTCCACA+AGG | - | chr8.1:10486044-10486063 | MS.gene58793:CDS | 45.0% |
| !! | CATGGATGATGGACAAGTTG+AGG | - | chr8.1:10485858-10485877 | MS.gene58793:CDS | 45.0% |
| CACCAATGGTCTTCCCTCAT+AGG | + | chr8.1:10485681-10485700 | None:intergenic | 50.0% | |
| GATGCTTACCTTCTTCGTCG+TGG | + | chr8.1:10485799-10485818 | None:intergenic | 50.0% | |
| TGAGGGAAGACCATTGGTGA+TGG | - | chr8.1:10485682-10485701 | MS.gene58793:CDS | 50.0% | |
| TGCCTATGAGGGAAGACCAT+TGG | - | chr8.1:10485676-10485695 | MS.gene58793:CDS | 50.0% | |
| TTCGAAGAGACCAACGGGTA+TGG | - | chr8.1:10485725-10485744 | MS.gene58793:CDS | 50.0% | |
| AGACCAACGGGTATGGATGC+GGG | - | chr8.1:10485732-10485751 | MS.gene58793:CDS | 55.0% | |
| GAGACCAACGGGTATGGATG+CGG | - | chr8.1:10485731-10485750 | MS.gene58793:CDS | 55.0% | |
| GTGGCGTTGCCACGATTTCA+AGG | + | chr8.1:10485780-10485799 | None:intergenic | 55.0% | |
| TCATTCTGGCGACGTGCATC+TGG | + | chr8.1:10486085-10486104 | None:intergenic | 55.0% | |
| TGGCGACGTGCATCTGGAAA+AGG | + | chr8.1:10486079-10486098 | None:intergenic | 55.0% | |
| AACGGGTATGGATGCGGGTG+CGG | - | chr8.1:10485737-10485756 | MS.gene58793:CDS | 60.0% | |
| TGGCAACGCCACGACGAAGA+AGG | - | chr8.1:10485788-10485807 | MS.gene58793:CDS | 60.0% | |
| GCACCCGCATCCATACCCGT+TGG | + | chr8.1:10485738-10485757 | None:intergenic | 65.0% | |
| GGTATGGATGCGGGTGCGGA+TGG | - | chr8.1:10485741-10485760 | MS.gene58793:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 10485671 | 10486111 | 10485671 | ID=MS.gene58793 |
| chr8.1 | mRNA | 10485671 | 10486111 | 10485671 | ID=MS.gene58793.t1;Parent=MS.gene58793 |
| chr8.1 | exon | 10485671 | 10486111 | 10485671 | ID=MS.gene58793.t1.exon1;Parent=MS.gene58793.t1 |
| chr8.1 | CDS | 10485671 | 10486111 | 10485671 | ID=cds.MS.gene58793.t1;Parent=MS.gene58793.t1 |
| Gene Sequence |
| Protein sequence |
