Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70277.t1 | XP_003630173.1 | 64 | 125 | 37 | 2 | 1 | 117 | 1 | 125 | 1.40E-26 | 129 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70277.t1 | G7LC90 | 64.0 | 125 | 37 | 2 | 1 | 117 | 1 | 125 | 1.0e-26 | 129.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene70277 | 0.816743 | 4.73E-52 | -1.69E-46 |
| MS.gene04998 | MS.gene70277 | 0.853685 | 2.03E-61 | -1.69E-46 |
| MS.gene051226 | MS.gene70277 | 0.819039 | 1.43E-52 | -1.69E-46 |
| MS.gene051676 | MS.gene70277 | 0.814052 | 1.87E-51 | -1.69E-46 |
| MS.gene051975 | MS.gene70277 | 0.801659 | 8.11E-49 | -1.69E-46 |
| MS.gene052533 | MS.gene70277 | 0.805687 | 1.18E-49 | -1.69E-46 |
| MS.gene053182 | MS.gene70277 | 0.804142 | 2.49E-49 | -1.69E-46 |
| MS.gene053298 | MS.gene70277 | 0.852536 | 4.33E-61 | -1.69E-46 |
| MS.gene053340 | MS.gene70277 | 0.803643 | 3.16E-49 | -1.69E-46 |
| MS.gene053963 | MS.gene70277 | 0.829004 | 6.56E-55 | -1.69E-46 |
| MS.gene054222 | MS.gene70277 | 0.805393 | 1.36E-49 | -1.69E-46 |
| MS.gene054892 | MS.gene70277 | 0.849159 | 3.87E-60 | -1.69E-46 |
| MS.gene054893 | MS.gene70277 | 0.853909 | 1.75E-61 | -1.69E-46 |
| MS.gene055226 | MS.gene70277 | 0.804686 | 1.92E-49 | -1.69E-46 |
| MS.gene056986 | MS.gene70277 | 0.80485 | 1.77E-49 | -1.69E-46 |
| MS.gene057155 | MS.gene70277 | 0.813865 | 2.06E-51 | -1.69E-46 |
| MS.gene057622 | MS.gene70277 | 0.823912 | 1.07E-53 | -1.69E-46 |
| MS.gene057999 | MS.gene70277 | 0.83326 | 5.91E-56 | -1.69E-46 |
| MS.gene058592 | MS.gene70277 | 0.820976 | 5.16E-53 | -1.69E-46 |
| MS.gene06023 | MS.gene70277 | 0.810357 | 1.20E-50 | -1.69E-46 |
| MS.gene060259 | MS.gene70277 | 0.814282 | 1.67E-51 | -1.69E-46 |
| MS.gene060412 | MS.gene70277 | 0.823216 | 1.56E-53 | -1.69E-46 |
| MS.gene060840 | MS.gene70277 | 0.801519 | 8.67E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70277.t1 | MTR_8g092580 | 88.000 | 125 | 7 | 2 | 1 | 117 | 1 | 125 | 1.07e-71 | 210 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAAAGATACAAGAGATCTT+TGG | 0.301924 | 4.1:+9007281 | MS.gene70277:CDS |
| TAGCACCTTCTTCTTCTTGT+TGG | 0.330250 | 4.1:+9007069 | MS.gene70277:CDS |
| CGATGCAGCACAAAAGCTTA+TGG | 0.339207 | 4.1:+9007145 | MS.gene70277:CDS |
| CTCACCCTTGTATCATTAAT+AGG | 0.341960 | 4.1:-9007383 | None:intergenic |
| CTCCTTTGCTTCTTAACTAT+AGG | 0.354880 | 4.1:-9007323 | None:intergenic |
| CAAAGATACAAGAGATCTTT+GGG | 0.370184 | 4.1:+9007282 | MS.gene70277:CDS |
| TTCTCTTGTGAATATTTACA+TGG | 0.455958 | 4.1:+9007352 | MS.gene70277:CDS |
| CTACACCTATTAATGATACA+AGG | 0.466346 | 4.1:+9007378 | MS.gene70277:CDS |
| TTCCTATAGTTAAGAAGCAA+AGG | 0.492711 | 4.1:+9007321 | MS.gene70277:CDS |
| AAGGGCAACAACAACATTAG+AGG | 0.505052 | 4.1:+9007185 | MS.gene70277:CDS |
| ACAAAGTGATGATATTGTAA+TGG | 0.520018 | 4.1:+9007259 | MS.gene70277:CDS |
| CGAAGCAACCGACATGAAGA+AGG | 0.528099 | 4.1:+9007227 | MS.gene70277:CDS |
| GGATCTTAGTGATGAAGAGA+AGG | 0.549113 | 4.1:+9007166 | MS.gene70277:CDS |
| TTGGCGTAAGATGAATATTG+TGG | 0.597025 | 4.1:+9007088 | MS.gene70277:CDS |
| GATCTTAGTGATGAAGAGAA+GGG | 0.605452 | 4.1:+9007167 | MS.gene70277:CDS |
| TTACGCCAACAAGAAGAAGA+AGG | 0.608118 | 4.1:-9007074 | None:intergenic |
| TACACCTATTAATGATACAA+GGG | 0.630937 | 4.1:+9007379 | MS.gene70277:CDS |
| TTAGAGGCAGAAGAAGCAAG+AGG | 0.689009 | 4.1:+9007201 | MS.gene70277:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACAAAGTGATGATATTGTAA+TGG | + | chr4.1:9007259-9007278 | MS.gene70277:CDS | 25.0% |
| ! | TACACCTATTAATGATACAA+GGG | + | chr4.1:9007379-9007398 | MS.gene70277:CDS | 25.0% |
| ! | TTCTCTTGTGAATATTTACA+TGG | + | chr4.1:9007352-9007371 | MS.gene70277:CDS | 25.0% |
| CAAAGATACAAGAGATCTTT+GGG | + | chr4.1:9007282-9007301 | MS.gene70277:CDS | 30.0% | |
| CTACACCTATTAATGATACA+AGG | + | chr4.1:9007378-9007397 | MS.gene70277:CDS | 30.0% | |
| TTCCTATAGTTAAGAAGCAA+AGG | + | chr4.1:9007321-9007340 | MS.gene70277:CDS | 30.0% | |
| CTCACCCTTGTATCATTAAT+AGG | - | chr4.1:9007386-9007405 | None:intergenic | 35.0% | |
| CTCCTTTGCTTCTTAACTAT+AGG | - | chr4.1:9007326-9007345 | None:intergenic | 35.0% | |
| GATCTTAGTGATGAAGAGAA+GGG | + | chr4.1:9007167-9007186 | MS.gene70277:CDS | 35.0% | |
| GCAAAGATACAAGAGATCTT+TGG | + | chr4.1:9007281-9007300 | MS.gene70277:CDS | 35.0% | |
| TTGGCGTAAGATGAATATTG+TGG | + | chr4.1:9007088-9007107 | MS.gene70277:CDS | 35.0% | |
| !!! | GCTTTTTTCCTTCTTCATGT+CGG | - | chr4.1:9007238-9007257 | None:intergenic | 35.0% |
| AAGGGCAACAACAACATTAG+AGG | + | chr4.1:9007185-9007204 | MS.gene70277:CDS | 40.0% | |
| GGATCTTAGTGATGAAGAGA+AGG | + | chr4.1:9007166-9007185 | MS.gene70277:CDS | 40.0% | |
| TAGCACCTTCTTCTTCTTGT+TGG | + | chr4.1:9007069-9007088 | MS.gene70277:CDS | 40.0% | |
| TTACGCCAACAAGAAGAAGA+AGG | - | chr4.1:9007077-9007096 | None:intergenic | 40.0% | |
| CGATGCAGCACAAAAGCTTA+TGG | + | chr4.1:9007145-9007164 | MS.gene70277:CDS | 45.0% | |
| TTAGAGGCAGAAGAAGCAAG+AGG | + | chr4.1:9007201-9007220 | MS.gene70277:CDS | 45.0% | |
| CGAAGCAACCGACATGAAGA+AGG | + | chr4.1:9007227-9007246 | MS.gene70277:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 9007059 | 9007412 | 9007059 | ID=MS.gene70277 |
| chr4.1 | mRNA | 9007059 | 9007412 | 9007059 | ID=MS.gene70277.t1;Parent=MS.gene70277 |
| chr4.1 | exon | 9007059 | 9007412 | 9007059 | ID=MS.gene70277.t1.exon1;Parent=MS.gene70277.t1 |
| chr4.1 | CDS | 9007059 | 9007412 | 9007059 | ID=cds.MS.gene70277.t1;Parent=MS.gene70277.t1 |
| Gene Sequence |
| Protein sequence |
