Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57704.t1 | GAU11971.1 | 83.9 | 149 | 18 | 4 | 1 | 146 | 1 | 146 | 4.30E-65 | 257.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57704.t1 | A0A392P6I4 | 84.6 | 149 | 17 | 4 | 1 | 146 | 1 | 146 | 1.2e-64 | 255.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04998 | MS.gene57704 | 0.888105 | 7.93E-73 | -1.69E-46 |
| MS.gene051226 | MS.gene57704 | 0.865717 | 4.82E-65 | -1.69E-46 |
| MS.gene051837 | MS.gene57704 | 0.804916 | 1.72E-49 | -1.69E-46 |
| MS.gene052296 | MS.gene57704 | 0.801006 | 1.10E-48 | -1.69E-46 |
| MS.gene052878 | MS.gene57704 | 0.800834 | 1.20E-48 | -1.69E-46 |
| MS.gene053182 | MS.gene57704 | 0.806714 | 7.19E-50 | -1.69E-46 |
| MS.gene054892 | MS.gene57704 | 0.818263 | 2.15E-52 | -1.69E-46 |
| MS.gene054893 | MS.gene57704 | 0.814629 | 1.40E-51 | -1.69E-46 |
| MS.gene055551 | MS.gene57704 | 0.803315 | 3.70E-49 | -1.69E-46 |
| MS.gene057093 | MS.gene57704 | 0.889029 | 3.49E-73 | -1.69E-46 |
| MS.gene057167 | MS.gene57704 | 0.826381 | 2.80E-54 | -1.69E-46 |
| MS.gene057432 | MS.gene57704 | 0.806851 | 6.73E-50 | -1.69E-46 |
| MS.gene057622 | MS.gene57704 | 0.838514 | 2.75E-57 | -1.69E-46 |
| MS.gene058592 | MS.gene57704 | 0.805096 | 1.57E-49 | -1.69E-46 |
| MS.gene06023 | MS.gene57704 | 0.835389 | 1.73E-56 | -1.69E-46 |
| MS.gene060259 | MS.gene57704 | 0.809931 | 1.48E-50 | -1.69E-46 |
| MS.gene060446 | MS.gene57704 | 0.809289 | 2.04E-50 | -1.69E-46 |
| MS.gene060964 | MS.gene57704 | 0.830019 | 3.72E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57704.t1 | MTR_4g116530 | 93.289 | 149 | 7 | 2 | 1 | 146 | 1 | 149 | 3.19e-100 | 284 |
| MS.gene57704.t1 | MTR_2g461190 | 59.459 | 148 | 49 | 6 | 1 | 145 | 1 | 140 | 8.60e-48 | 151 |
| MS.gene57704.t1 | MTR_4g106650 | 39.130 | 69 | 42 | 0 | 66 | 134 | 62 | 130 | 5.15e-12 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57704.t1 | AT5G25240 | 39.024 | 123 | 56 | 6 | 17 | 127 | 11 | 126 | 1.21e-20 | 82.0 |
| MS.gene57704.t1 | AT5G14895 | 38.202 | 89 | 42 | 3 | 61 | 136 | 60 | 148 | 6.44e-12 | 60.5 |
Find 39 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTAAGTGGAAAACGTTTCTT+AGG | 0.293276 | 8.3:-9406286 | MS.gene57704:CDS |
| CTTAGGAAAATTAGTGGGTA+TGG | 0.339302 | 8.3:-9406269 | MS.gene57704:CDS |
| TATGCTCTTAATTTCAATAG+TGG | 0.374345 | 8.3:-9406206 | MS.gene57704:CDS |
| TCATTCTGGCGACGTGCATC+TGG | 0.387586 | 8.3:+9406119 | None:intergenic |
| ATATATATCATGTTTCATTC+TGG | 0.388657 | 8.3:+9406105 | None:intergenic |
| AAACAACATGGCTGCCTATG+AGG | 0.423463 | 8.3:-9406537 | None:intergenic |
| CACCAATGGTCTTCCCTCAT+AGG | 0.431252 | 8.3:+9406523 | None:intergenic |
| ATGCTCTTAATTTCAATAGT+GGG | 0.436223 | 8.3:-9406205 | MS.gene57704:CDS |
| AACGGGTATGGATGCGGGTG+CGG | 0.443003 | 8.3:-9406464 | MS.gene57704:CDS |
| AAGACCATTGGTGATGGAAA+TGG | 0.450625 | 8.3:-9406513 | MS.gene57704:CDS |
| GAAAATAGTGAAGGAAATAG+AGG | 0.458494 | 8.3:-9406374 | MS.gene57704:CDS |
| TGGCGACGTGCATCTGGAAA+AGG | 0.467598 | 8.3:+9406125 | None:intergenic |
| CTTTCCATTTCCATCACCAA+TGG | 0.472269 | 8.3:+9406509 | None:intergenic |
| TTAGGAAAATTAGTGGGTAT+GGG | 0.477370 | 8.3:-9406268 | MS.gene57704:CDS |
| GTGGCGTTGCCACGATTTCA+AGG | 0.486009 | 8.3:+9406424 | None:intergenic |
| TTCGAAGAGACCAACGGGTA+TGG | 0.486097 | 8.3:-9406476 | MS.gene57704:CDS |
| GAGACTTCAGAAGTGATTGC+TGG | 0.496587 | 8.3:-9406311 | MS.gene57704:CDS |
| GGTATGGATGCGGGTGCGGA+TGG | 0.500992 | 8.3:-9406460 | MS.gene57704:CDS |
| AAGATTTCGAAGAGACCAAC+GGG | 0.512584 | 8.3:-9406481 | MS.gene57704:CDS |
| GAGACCAACGGGTATGGATG+CGG | 0.514745 | 8.3:-9406470 | MS.gene57704:CDS |
| AGACCAACGGGTATGGATGC+GGG | 0.516345 | 8.3:-9406469 | MS.gene57704:CDS |
| AGGAGAAGAGACATGGATGA+TGG | 0.526434 | 8.3:-9406354 | MS.gene57704:CDS |
| TGCTCTTAATTTCAATAGTG+GGG | 0.526773 | 8.3:-9406204 | MS.gene57704:CDS |
| ACGTTTCTTAGGAAAATTAG+TGG | 0.531357 | 8.3:-9406275 | MS.gene57704:CDS |
| GGACAAGTTGAGGAAGATGA+AGG | 0.537471 | 8.3:-9406333 | MS.gene57704:CDS |
| AACAACATGGCTGCCTATGA+GGG | 0.543567 | 8.3:-9406536 | None:intergenic |
| TGGCAACGCCACGACGAAGA+AGG | 0.555768 | 8.3:-9406413 | MS.gene57704:CDS |
| GAAATAGAGGAGAAGAGACA+TGG | 0.562044 | 8.3:-9406361 | MS.gene57704:CDS |
| AAGTGATTGCTGGTCCTAAG+TGG | 0.571200 | 8.3:-9406301 | MS.gene57704:CDS |
| AAATATTCACCTTGAAATCG+TGG | 0.578098 | 8.3:-9406433 | MS.gene57704:CDS |
| CATGGATGATGGACAAGTTG+AGG | 0.582027 | 8.3:-9406343 | MS.gene57704:CDS |
| CGTTTCTTAGGAAAATTAGT+GGG | 0.584307 | 8.3:-9406274 | MS.gene57704:CDS |
| TGAGGGAAGACCATTGGTGA+TGG | 0.585967 | 8.3:-9406519 | MS.gene57704:CDS |
| CTACTTAATGAAAATAGTGA+AGG | 0.588080 | 8.3:-9406383 | MS.gene57704:CDS |
| TGCCTATGAGGGAAGACCAT+TGG | 0.600498 | 8.3:-9406525 | MS.gene57704:CDS |
| CAAGATTTCGAAGAGACCAA+CGG | 0.625236 | 8.3:-9406482 | MS.gene57704:CDS |
| GATGCTTACCTTCTTCGTCG+TGG | 0.640185 | 8.3:+9406405 | None:intergenic |
| AAAAGGATTAGAAAACCTTG+TGG | 0.651946 | 8.3:+9406142 | None:intergenic |
| GCACCCGCATCCATACCCGT+TGG | 0.669123 | 8.3:+9406466 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGCTCTTAATTTCAATAGT+GGG | - | chr8.3:9406437-9406456 | MS.gene57704:CDS | 25.0% |
| ! | CTACTTAATGAAAATAGTGA+AGG | - | chr8.3:9406259-9406278 | MS.gene57704:CDS | 25.0% |
| ! | TATGCTCTTAATTTCAATAG+TGG | - | chr8.3:9406436-9406455 | MS.gene57704:CDS | 25.0% |
| AAAAGGATTAGAAAACCTTG+TGG | + | chr8.3:9406503-9406522 | None:intergenic | 30.0% | |
| AAATATTCACCTTGAAATCG+TGG | - | chr8.3:9406209-9406228 | MS.gene57704:CDS | 30.0% | |
| ACGTTTCTTAGGAAAATTAG+TGG | - | chr8.3:9406367-9406386 | MS.gene57704:CDS | 30.0% | |
| CGTTTCTTAGGAAAATTAGT+GGG | - | chr8.3:9406368-9406387 | MS.gene57704:CDS | 30.0% | |
| GAAAATAGTGAAGGAAATAG+AGG | - | chr8.3:9406268-9406287 | MS.gene57704:CDS | 30.0% | |
| TGCTCTTAATTTCAATAGTG+GGG | - | chr8.3:9406438-9406457 | MS.gene57704:CDS | 30.0% | |
| ! | ATGGGAAAAAACAAAAGCAT+AGG | - | chr8.3:9406392-9406411 | MS.gene57704:CDS | 30.0% |
| ! | TTAGGAAAATTAGTGGGTAT+GGG | - | chr8.3:9406374-9406393 | MS.gene57704:CDS | 30.0% |
| CTAAGTGGAAAACGTTTCTT+AGG | - | chr8.3:9406356-9406375 | MS.gene57704:CDS | 35.0% | |
| GAAAACCTTGTGGAAAAACT+TGG | + | chr8.3:9406493-9406512 | None:intergenic | 35.0% | |
| ! | CTAAGAAACGTTTTCCACTT+AGG | + | chr8.3:9406358-9406377 | None:intergenic | 35.0% |
| ! | CTTAGGAAAATTAGTGGGTA+TGG | - | chr8.3:9406373-9406392 | MS.gene57704:CDS | 35.0% |
| AACCTTGTGGAAAAACTTGG+AGG | + | chr8.3:9406490-9406509 | None:intergenic | 40.0% | |
| AAGATTTCGAAGAGACCAAC+GGG | - | chr8.3:9406161-9406180 | MS.gene57704:CDS | 40.0% | |
| CAAGATTTCGAAGAGACCAA+CGG | - | chr8.3:9406160-9406179 | MS.gene57704:CDS | 40.0% | |
| CTTTCCATTTCCATCACCAA+TGG | + | chr8.3:9406136-9406155 | None:intergenic | 40.0% | |
| GAAATAGAGGAGAAGAGACA+TGG | - | chr8.3:9406281-9406300 | MS.gene57704:CDS | 40.0% | |
| !! | AAGACCATTGGTGATGGAAA+TGG | - | chr8.3:9406129-9406148 | MS.gene57704:CDS | 40.0% |
| AAGTGATTGCTGGTCCTAAG+TGG | - | chr8.3:9406341-9406360 | MS.gene57704:CDS | 45.0% | |
| AGGAGAAGAGACATGGATGA+TGG | - | chr8.3:9406288-9406307 | MS.gene57704:CDS | 45.0% | |
| GAGACTTCAGAAGTGATTGC+TGG | - | chr8.3:9406331-9406350 | MS.gene57704:CDS | 45.0% | |
| ! | GGACAAGTTGAGGAAGATGA+AGG | - | chr8.3:9406309-9406328 | MS.gene57704:CDS | 45.0% |
| ! | TGCCTCCAAGTTTTTCCACA+AGG | - | chr8.3:9406485-9406504 | MS.gene57704:CDS | 45.0% |
| !! | CATGGATGATGGACAAGTTG+AGG | - | chr8.3:9406299-9406318 | MS.gene57704:CDS | 45.0% |
| CACCAATGGTCTTCCCTCAT+AGG | + | chr8.3:9406122-9406141 | None:intergenic | 50.0% | |
| GATGCTTACCTTCTTCGTCG+TGG | + | chr8.3:9406240-9406259 | None:intergenic | 50.0% | |
| TGAGGGAAGACCATTGGTGA+TGG | - | chr8.3:9406123-9406142 | MS.gene57704:CDS | 50.0% | |
| TGCCTATGAGGGAAGACCAT+TGG | - | chr8.3:9406117-9406136 | MS.gene57704:CDS | 50.0% | |
| TTCGAAGAGACCAACGGGTA+TGG | - | chr8.3:9406166-9406185 | MS.gene57704:CDS | 50.0% | |
| AGACCAACGGGTATGGATGC+GGG | - | chr8.3:9406173-9406192 | MS.gene57704:CDS | 55.0% | |
| GAGACCAACGGGTATGGATG+CGG | - | chr8.3:9406172-9406191 | MS.gene57704:CDS | 55.0% | |
| GTGGCGTTGCCACGATTTCA+AGG | + | chr8.3:9406221-9406240 | None:intergenic | 55.0% | |
| TCATTCTGGCGACGTGCATC+TGG | + | chr8.3:9406526-9406545 | None:intergenic | 55.0% | |
| TGGCGACGTGCATCTGGAAA+AGG | + | chr8.3:9406520-9406539 | None:intergenic | 55.0% | |
| AACGGGTATGGATGCGGGTG+CGG | - | chr8.3:9406178-9406197 | MS.gene57704:CDS | 60.0% | |
| TGGCAACGCCACGACGAAGA+AGG | - | chr8.3:9406229-9406248 | MS.gene57704:CDS | 60.0% | |
| GCACCCGCATCCATACCCGT+TGG | + | chr8.3:9406179-9406198 | None:intergenic | 65.0% | |
| GGTATGGATGCGGGTGCGGA+TGG | - | chr8.3:9406182-9406201 | MS.gene57704:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 9406112 | 9406552 | 9406112 | ID=MS.gene57704 |
| chr8.3 | mRNA | 9406112 | 9406552 | 9406112 | ID=MS.gene57704.t1;Parent=MS.gene57704 |
| chr8.3 | exon | 9406112 | 9406552 | 9406112 | ID=MS.gene57704.t1.exon1;Parent=MS.gene57704.t1 |
| chr8.3 | CDS | 9406112 | 9406552 | 9406112 | ID=cds.MS.gene57704.t1;Parent=MS.gene57704.t1 |
| Gene Sequence |
| Protein sequence |
