Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58768.t1 | GAU11971.1 | 83.9 | 149 | 18 | 4 | 1 | 146 | 1 | 146 | 4.30E-65 | 257.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58768.t1 | A0A392P6I4 | 84.6 | 149 | 17 | 4 | 1 | 146 | 1 | 146 | 1.2e-64 | 255.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04998 | MS.gene58768 | 0.901848 | 1.77E-78 | -1.69E-46 |
| MS.gene051226 | MS.gene58768 | 0.876896 | 9.69E-69 | -1.69E-46 |
| MS.gene051837 | MS.gene58768 | 0.826249 | 3.01E-54 | -1.69E-46 |
| MS.gene052296 | MS.gene58768 | 0.823042 | 1.71E-53 | -1.69E-46 |
| MS.gene052878 | MS.gene58768 | 0.8113 | 7.50E-51 | -1.69E-46 |
| MS.gene053182 | MS.gene58768 | 0.811686 | 6.18E-51 | -1.69E-46 |
| MS.gene053879 | MS.gene58768 | 0.813011 | 3.18E-51 | -1.69E-46 |
| MS.gene054892 | MS.gene58768 | 0.840366 | 9.08E-58 | -1.69E-46 |
| MS.gene054893 | MS.gene58768 | 0.836037 | 1.18E-56 | -1.69E-46 |
| MS.gene055551 | MS.gene58768 | 0.802105 | 6.57E-49 | -1.69E-46 |
| MS.gene056992 | MS.gene58768 | 0.812506 | 4.10E-51 | -1.69E-46 |
| MS.gene057093 | MS.gene58768 | 0.892928 | 1.01E-74 | -1.69E-46 |
| MS.gene057167 | MS.gene58768 | 0.842006 | 3.37E-58 | -1.69E-46 |
| MS.gene057432 | MS.gene58768 | 0.82053 | 6.53E-53 | -1.69E-46 |
| MS.gene057622 | MS.gene58768 | 0.853896 | 1.76E-61 | -1.69E-46 |
| MS.gene057999 | MS.gene58768 | 0.807063 | 6.07E-50 | -1.69E-46 |
| MS.gene058592 | MS.gene58768 | 0.822588 | 2.19E-53 | -1.69E-46 |
| MS.gene06023 | MS.gene58768 | 0.846551 | 2.02E-59 | -1.69E-46 |
| MS.gene060259 | MS.gene58768 | 0.819761 | 9.80E-53 | -1.69E-46 |
| MS.gene060446 | MS.gene58768 | 0.825572 | 4.36E-54 | -1.69E-46 |
| MS.gene060964 | MS.gene58768 | 0.841888 | 3.61E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58768.t1 | MTR_4g116530 | 93.289 | 149 | 7 | 2 | 1 | 146 | 1 | 149 | 3.19e-100 | 284 |
| MS.gene58768.t1 | MTR_2g461190 | 59.459 | 148 | 49 | 6 | 1 | 145 | 1 | 140 | 8.60e-48 | 151 |
| MS.gene58768.t1 | MTR_4g106650 | 39.130 | 69 | 42 | 0 | 66 | 134 | 62 | 130 | 5.15e-12 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58768.t1 | AT5G25240 | 39.024 | 123 | 56 | 6 | 17 | 127 | 11 | 126 | 1.21e-20 | 82.0 |
| MS.gene58768.t1 | AT5G14895 | 38.202 | 89 | 42 | 3 | 61 | 136 | 60 | 148 | 6.44e-12 | 60.5 |
Find 39 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTAAGTGGAAAACGTTTCTT+AGG | 0.293276 | 8.1:-10419806 | MS.gene58768:CDS |
| CTTAGGAAAATTAGTGGGTA+TGG | 0.339302 | 8.1:-10419789 | MS.gene58768:CDS |
| TATGCTCTTAATTTCAATAG+TGG | 0.374345 | 8.1:-10419726 | MS.gene58768:CDS |
| TCATTCTGGCGACGTGCATC+TGG | 0.387586 | 8.1:+10419639 | None:intergenic |
| ATATATATCATGTTTCATTC+TGG | 0.388657 | 8.1:+10419625 | None:intergenic |
| AAACAACATGGCTGCCTATG+AGG | 0.423463 | 8.1:-10420057 | None:intergenic |
| CACCAATGGTCTTCCCTCAT+AGG | 0.431252 | 8.1:+10420043 | None:intergenic |
| ATGCTCTTAATTTCAATAGT+GGG | 0.436223 | 8.1:-10419725 | MS.gene58768:CDS |
| AACGGGTATGGATGCGGGTG+CGG | 0.443003 | 8.1:-10419984 | MS.gene58768:CDS |
| AAGACCATTGGTGATGGAAA+TGG | 0.450625 | 8.1:-10420033 | MS.gene58768:CDS |
| GAAAATAGTGAAGGAAATAG+AGG | 0.458494 | 8.1:-10419894 | MS.gene58768:CDS |
| TGGCGACGTGCATCTGGAAA+AGG | 0.467598 | 8.1:+10419645 | None:intergenic |
| CTTTCCATTTCCATCACCAA+TGG | 0.472269 | 8.1:+10420029 | None:intergenic |
| TTAGGAAAATTAGTGGGTAT+GGG | 0.477370 | 8.1:-10419788 | MS.gene58768:CDS |
| GTGGCGTTGCCACGATTTCA+AGG | 0.486009 | 8.1:+10419944 | None:intergenic |
| TTCGAAGAGACCAACGGGTA+TGG | 0.486097 | 8.1:-10419996 | MS.gene58768:CDS |
| GAGACTTCAGAAGTGATTGC+TGG | 0.496587 | 8.1:-10419831 | MS.gene58768:CDS |
| GGTATGGATGCGGGTGCGGA+TGG | 0.500992 | 8.1:-10419980 | MS.gene58768:CDS |
| AAGATTTCGAAGAGACCAAC+GGG | 0.512584 | 8.1:-10420001 | MS.gene58768:CDS |
| GAGACCAACGGGTATGGATG+CGG | 0.514745 | 8.1:-10419990 | MS.gene58768:CDS |
| AGACCAACGGGTATGGATGC+GGG | 0.516345 | 8.1:-10419989 | MS.gene58768:CDS |
| AGGAGAAGAGACATGGATGA+TGG | 0.526434 | 8.1:-10419874 | MS.gene58768:CDS |
| TGCTCTTAATTTCAATAGTG+GGG | 0.526773 | 8.1:-10419724 | MS.gene58768:CDS |
| ACGTTTCTTAGGAAAATTAG+TGG | 0.531357 | 8.1:-10419795 | MS.gene58768:CDS |
| GGACAAGTTGAGGAAGATGA+AGG | 0.537471 | 8.1:-10419853 | MS.gene58768:CDS |
| AACAACATGGCTGCCTATGA+GGG | 0.543567 | 8.1:-10420056 | None:intergenic |
| TGGCAACGCCACGACGAAGA+AGG | 0.555768 | 8.1:-10419933 | MS.gene58768:CDS |
| GAAATAGAGGAGAAGAGACA+TGG | 0.562044 | 8.1:-10419881 | MS.gene58768:CDS |
| AAGTGATTGCTGGTCCTAAG+TGG | 0.571200 | 8.1:-10419821 | MS.gene58768:CDS |
| AAATATTCACCTTGAAATCG+TGG | 0.578098 | 8.1:-10419953 | MS.gene58768:CDS |
| CATGGATGATGGACAAGTTG+AGG | 0.582027 | 8.1:-10419863 | MS.gene58768:CDS |
| CGTTTCTTAGGAAAATTAGT+GGG | 0.584307 | 8.1:-10419794 | MS.gene58768:CDS |
| TGAGGGAAGACCATTGGTGA+TGG | 0.585967 | 8.1:-10420039 | MS.gene58768:CDS |
| CTACTTAATGAAAATAGTGA+AGG | 0.588080 | 8.1:-10419903 | MS.gene58768:CDS |
| TGCCTATGAGGGAAGACCAT+TGG | 0.600498 | 8.1:-10420045 | MS.gene58768:CDS |
| CAAGATTTCGAAGAGACCAA+CGG | 0.625236 | 8.1:-10420002 | MS.gene58768:CDS |
| GATGCTTACCTTCTTCGTCG+TGG | 0.640185 | 8.1:+10419925 | None:intergenic |
| AAAAGGATTAGAAAACCTTG+TGG | 0.651946 | 8.1:+10419662 | None:intergenic |
| GCACCCGCATCCATACCCGT+TGG | 0.669123 | 8.1:+10419986 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGCTCTTAATTTCAATAGT+GGG | - | chr8.1:10419957-10419976 | MS.gene58768:CDS | 25.0% |
| ! | CTACTTAATGAAAATAGTGA+AGG | - | chr8.1:10419779-10419798 | MS.gene58768:CDS | 25.0% |
| ! | TATGCTCTTAATTTCAATAG+TGG | - | chr8.1:10419956-10419975 | MS.gene58768:CDS | 25.0% |
| AAAAGGATTAGAAAACCTTG+TGG | + | chr8.1:10420023-10420042 | None:intergenic | 30.0% | |
| AAATATTCACCTTGAAATCG+TGG | - | chr8.1:10419729-10419748 | MS.gene58768:CDS | 30.0% | |
| ACGTTTCTTAGGAAAATTAG+TGG | - | chr8.1:10419887-10419906 | MS.gene58768:CDS | 30.0% | |
| CGTTTCTTAGGAAAATTAGT+GGG | - | chr8.1:10419888-10419907 | MS.gene58768:CDS | 30.0% | |
| GAAAATAGTGAAGGAAATAG+AGG | - | chr8.1:10419788-10419807 | MS.gene58768:CDS | 30.0% | |
| TGCTCTTAATTTCAATAGTG+GGG | - | chr8.1:10419958-10419977 | MS.gene58768:CDS | 30.0% | |
| ! | ATGGGAAAAAACAAAAGCAT+AGG | - | chr8.1:10419912-10419931 | MS.gene58768:CDS | 30.0% |
| ! | TTAGGAAAATTAGTGGGTAT+GGG | - | chr8.1:10419894-10419913 | MS.gene58768:CDS | 30.0% |
| CTAAGTGGAAAACGTTTCTT+AGG | - | chr8.1:10419876-10419895 | MS.gene58768:CDS | 35.0% | |
| GAAAACCTTGTGGAAAAACT+TGG | + | chr8.1:10420013-10420032 | None:intergenic | 35.0% | |
| ! | CTAAGAAACGTTTTCCACTT+AGG | + | chr8.1:10419878-10419897 | None:intergenic | 35.0% |
| ! | CTTAGGAAAATTAGTGGGTA+TGG | - | chr8.1:10419893-10419912 | MS.gene58768:CDS | 35.0% |
| AACCTTGTGGAAAAACTTGG+AGG | + | chr8.1:10420010-10420029 | None:intergenic | 40.0% | |
| AAGATTTCGAAGAGACCAAC+GGG | - | chr8.1:10419681-10419700 | MS.gene58768:CDS | 40.0% | |
| CAAGATTTCGAAGAGACCAA+CGG | - | chr8.1:10419680-10419699 | MS.gene58768:CDS | 40.0% | |
| CTTTCCATTTCCATCACCAA+TGG | + | chr8.1:10419656-10419675 | None:intergenic | 40.0% | |
| GAAATAGAGGAGAAGAGACA+TGG | - | chr8.1:10419801-10419820 | MS.gene58768:CDS | 40.0% | |
| !! | AAGACCATTGGTGATGGAAA+TGG | - | chr8.1:10419649-10419668 | MS.gene58768:CDS | 40.0% |
| AAGTGATTGCTGGTCCTAAG+TGG | - | chr8.1:10419861-10419880 | MS.gene58768:CDS | 45.0% | |
| AGGAGAAGAGACATGGATGA+TGG | - | chr8.1:10419808-10419827 | MS.gene58768:CDS | 45.0% | |
| GAGACTTCAGAAGTGATTGC+TGG | - | chr8.1:10419851-10419870 | MS.gene58768:CDS | 45.0% | |
| ! | GGACAAGTTGAGGAAGATGA+AGG | - | chr8.1:10419829-10419848 | MS.gene58768:CDS | 45.0% |
| ! | TGCCTCCAAGTTTTTCCACA+AGG | - | chr8.1:10420005-10420024 | MS.gene58768:CDS | 45.0% |
| !! | CATGGATGATGGACAAGTTG+AGG | - | chr8.1:10419819-10419838 | MS.gene58768:CDS | 45.0% |
| CACCAATGGTCTTCCCTCAT+AGG | + | chr8.1:10419642-10419661 | None:intergenic | 50.0% | |
| GATGCTTACCTTCTTCGTCG+TGG | + | chr8.1:10419760-10419779 | None:intergenic | 50.0% | |
| TGAGGGAAGACCATTGGTGA+TGG | - | chr8.1:10419643-10419662 | MS.gene58768:CDS | 50.0% | |
| TGCCTATGAGGGAAGACCAT+TGG | - | chr8.1:10419637-10419656 | MS.gene58768:CDS | 50.0% | |
| TTCGAAGAGACCAACGGGTA+TGG | - | chr8.1:10419686-10419705 | MS.gene58768:CDS | 50.0% | |
| AGACCAACGGGTATGGATGC+GGG | - | chr8.1:10419693-10419712 | MS.gene58768:CDS | 55.0% | |
| GAGACCAACGGGTATGGATG+CGG | - | chr8.1:10419692-10419711 | MS.gene58768:CDS | 55.0% | |
| GTGGCGTTGCCACGATTTCA+AGG | + | chr8.1:10419741-10419760 | None:intergenic | 55.0% | |
| TCATTCTGGCGACGTGCATC+TGG | + | chr8.1:10420046-10420065 | None:intergenic | 55.0% | |
| TGGCGACGTGCATCTGGAAA+AGG | + | chr8.1:10420040-10420059 | None:intergenic | 55.0% | |
| AACGGGTATGGATGCGGGTG+CGG | - | chr8.1:10419698-10419717 | MS.gene58768:CDS | 60.0% | |
| TGGCAACGCCACGACGAAGA+AGG | - | chr8.1:10419749-10419768 | MS.gene58768:CDS | 60.0% | |
| GCACCCGCATCCATACCCGT+TGG | + | chr8.1:10419699-10419718 | None:intergenic | 65.0% | |
| GGTATGGATGCGGGTGCGGA+TGG | - | chr8.1:10419702-10419721 | MS.gene58768:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 10419632 | 10420072 | 10419632 | ID=MS.gene58768 |
| chr8.1 | mRNA | 10419632 | 10420072 | 10419632 | ID=MS.gene58768.t1;Parent=MS.gene58768 |
| chr8.1 | exon | 10419632 | 10420072 | 10419632 | ID=MS.gene58768.t1.exon1;Parent=MS.gene58768.t1 |
| chr8.1 | CDS | 10419632 | 10420072 | 10419632 | ID=cds.MS.gene58768.t1;Parent=MS.gene58768.t1 |
| Gene Sequence |
| Protein sequence |
