Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58769.t1 | GAU11971.1 | 83.9 | 149 | 18 | 4 | 1 | 146 | 1 | 146 | 4.30E-65 | 257.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58769.t1 | A0A392P6I4 | 84.6 | 149 | 17 | 4 | 1 | 146 | 1 | 146 | 1.2e-64 | 255.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04998 | MS.gene58769 | 0.896609 | 3.13E-76 | -1.69E-46 |
| MS.gene051226 | MS.gene58769 | 0.872535 | 2.95E-67 | -1.69E-46 |
| MS.gene051837 | MS.gene58769 | 0.821123 | 4.77E-53 | -1.69E-46 |
| MS.gene052296 | MS.gene58769 | 0.8142 | 1.74E-51 | -1.69E-46 |
| MS.gene052878 | MS.gene58769 | 0.802683 | 4.99E-49 | -1.69E-46 |
| MS.gene053182 | MS.gene58769 | 0.810367 | 1.19E-50 | -1.69E-46 |
| MS.gene053879 | MS.gene58769 | 0.80468 | 1.92E-49 | -1.69E-46 |
| MS.gene054892 | MS.gene58769 | 0.835132 | 2.00E-56 | -1.69E-46 |
| MS.gene054893 | MS.gene58769 | 0.830228 | 3.30E-55 | -1.69E-46 |
| MS.gene055551 | MS.gene58769 | 0.800249 | 1.57E-48 | -1.69E-46 |
| MS.gene056992 | MS.gene58769 | 0.810805 | 9.60E-51 | -1.69E-46 |
| MS.gene057093 | MS.gene58769 | 0.892247 | 1.89E-74 | -1.69E-46 |
| MS.gene057167 | MS.gene58769 | 0.842473 | 2.53E-58 | -1.69E-46 |
| MS.gene057432 | MS.gene58769 | 0.812471 | 4.17E-51 | -1.69E-46 |
| MS.gene057622 | MS.gene58769 | 0.851015 | 1.17E-60 | -1.69E-46 |
| MS.gene057999 | MS.gene58769 | 0.801254 | 9.82E-49 | -1.69E-46 |
| MS.gene058592 | MS.gene58769 | 0.810898 | 9.17E-51 | -1.69E-46 |
| MS.gene06023 | MS.gene58769 | 0.839884 | 1.21E-57 | -1.69E-46 |
| MS.gene060259 | MS.gene58769 | 0.817242 | 3.65E-52 | -1.69E-46 |
| MS.gene060446 | MS.gene58769 | 0.816538 | 5.25E-52 | -1.69E-46 |
| MS.gene060964 | MS.gene58769 | 0.835268 | 1.85E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58769.t1 | MTR_4g116530 | 93.289 | 149 | 7 | 2 | 1 | 146 | 1 | 149 | 3.19e-100 | 284 |
| MS.gene58769.t1 | MTR_2g461190 | 59.459 | 148 | 49 | 6 | 1 | 145 | 1 | 140 | 8.60e-48 | 151 |
| MS.gene58769.t1 | MTR_4g106650 | 39.130 | 69 | 42 | 0 | 66 | 134 | 62 | 130 | 5.15e-12 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58769.t1 | AT5G25240 | 39.024 | 123 | 56 | 6 | 17 | 127 | 11 | 126 | 1.21e-20 | 82.0 |
| MS.gene58769.t1 | AT5G14895 | 38.202 | 89 | 42 | 3 | 61 | 136 | 60 | 148 | 6.44e-12 | 60.5 |
Find 39 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTAAGTGGAAAACGTTTCTT+AGG | 0.293276 | 8.3:-9449729 | MS.gene58769:CDS |
| CTTAGGAAAATTAGTGGGTA+TGG | 0.339302 | 8.3:-9449712 | MS.gene58769:CDS |
| TATGCTCTTAATTTCAATAG+TGG | 0.374345 | 8.3:-9449649 | MS.gene58769:CDS |
| TCATTCTGGCGACGTGCATC+TGG | 0.387586 | 8.3:+9449562 | None:intergenic |
| ATATATATCATGTTTCATTC+TGG | 0.388657 | 8.3:+9449548 | None:intergenic |
| AAACAACATGGCTGCCTATG+AGG | 0.423463 | 8.3:-9449980 | None:intergenic |
| CACCAATGGTCTTCCCTCAT+AGG | 0.431252 | 8.3:+9449966 | None:intergenic |
| ATGCTCTTAATTTCAATAGT+GGG | 0.436223 | 8.3:-9449648 | MS.gene58769:CDS |
| AACGGGTATGGATGCGGGTG+CGG | 0.443003 | 8.3:-9449907 | MS.gene58769:CDS |
| AAGACCATTGGTGATGGAAA+TGG | 0.450625 | 8.3:-9449956 | MS.gene58769:CDS |
| GAAAATAGTGAAGGAAATAG+AGG | 0.458494 | 8.3:-9449817 | MS.gene58769:CDS |
| TGGCGACGTGCATCTGGAAA+AGG | 0.467598 | 8.3:+9449568 | None:intergenic |
| CTTTCCATTTCCATCACCAA+TGG | 0.472269 | 8.3:+9449952 | None:intergenic |
| TTAGGAAAATTAGTGGGTAT+GGG | 0.477370 | 8.3:-9449711 | MS.gene58769:CDS |
| GTGGCGTTGCCACGATTTCA+AGG | 0.486009 | 8.3:+9449867 | None:intergenic |
| TTCGAAGAGACCAACGGGTA+TGG | 0.486097 | 8.3:-9449919 | MS.gene58769:CDS |
| GAGACTTCAGAAGTGATTGC+TGG | 0.496587 | 8.3:-9449754 | MS.gene58769:CDS |
| GGTATGGATGCGGGTGCGGA+TGG | 0.500992 | 8.3:-9449903 | MS.gene58769:CDS |
| AAGATTTCGAAGAGACCAAC+GGG | 0.512584 | 8.3:-9449924 | MS.gene58769:CDS |
| GAGACCAACGGGTATGGATG+CGG | 0.514745 | 8.3:-9449913 | MS.gene58769:CDS |
| AGACCAACGGGTATGGATGC+GGG | 0.516345 | 8.3:-9449912 | MS.gene58769:CDS |
| AGGAGAAGAGACATGGATGA+TGG | 0.526434 | 8.3:-9449797 | MS.gene58769:CDS |
| TGCTCTTAATTTCAATAGTG+GGG | 0.526773 | 8.3:-9449647 | MS.gene58769:CDS |
| ACGTTTCTTAGGAAAATTAG+TGG | 0.531357 | 8.3:-9449718 | MS.gene58769:CDS |
| GGACAAGTTGAGGAAGATGA+AGG | 0.537471 | 8.3:-9449776 | MS.gene58769:CDS |
| AACAACATGGCTGCCTATGA+GGG | 0.543567 | 8.3:-9449979 | None:intergenic |
| TGGCAACGCCACGACGAAGA+AGG | 0.555768 | 8.3:-9449856 | MS.gene58769:CDS |
| GAAATAGAGGAGAAGAGACA+TGG | 0.562044 | 8.3:-9449804 | MS.gene58769:CDS |
| AAGTGATTGCTGGTCCTAAG+TGG | 0.571200 | 8.3:-9449744 | MS.gene58769:CDS |
| AAATATTCACCTTGAAATCG+TGG | 0.578098 | 8.3:-9449876 | MS.gene58769:CDS |
| CATGGATGATGGACAAGTTG+AGG | 0.582027 | 8.3:-9449786 | MS.gene58769:CDS |
| CGTTTCTTAGGAAAATTAGT+GGG | 0.584307 | 8.3:-9449717 | MS.gene58769:CDS |
| TGAGGGAAGACCATTGGTGA+TGG | 0.585967 | 8.3:-9449962 | MS.gene58769:CDS |
| CTACTTAATGAAAATAGTGA+AGG | 0.588080 | 8.3:-9449826 | MS.gene58769:CDS |
| TGCCTATGAGGGAAGACCAT+TGG | 0.600498 | 8.3:-9449968 | MS.gene58769:CDS |
| CAAGATTTCGAAGAGACCAA+CGG | 0.625236 | 8.3:-9449925 | MS.gene58769:CDS |
| GATGCTTACCTTCTTCGTCG+TGG | 0.640185 | 8.3:+9449848 | None:intergenic |
| AAAAGGATTAGAAAACCTTG+TGG | 0.651946 | 8.3:+9449585 | None:intergenic |
| GCACCCGCATCCATACCCGT+TGG | 0.669123 | 8.3:+9449909 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGCTCTTAATTTCAATAGT+GGG | - | chr8.3:9449880-9449899 | MS.gene58769:CDS | 25.0% |
| ! | CTACTTAATGAAAATAGTGA+AGG | - | chr8.3:9449702-9449721 | MS.gene58769:CDS | 25.0% |
| ! | TATGCTCTTAATTTCAATAG+TGG | - | chr8.3:9449879-9449898 | MS.gene58769:CDS | 25.0% |
| AAAAGGATTAGAAAACCTTG+TGG | + | chr8.3:9449946-9449965 | None:intergenic | 30.0% | |
| AAATATTCACCTTGAAATCG+TGG | - | chr8.3:9449652-9449671 | MS.gene58769:CDS | 30.0% | |
| ACGTTTCTTAGGAAAATTAG+TGG | - | chr8.3:9449810-9449829 | MS.gene58769:CDS | 30.0% | |
| CGTTTCTTAGGAAAATTAGT+GGG | - | chr8.3:9449811-9449830 | MS.gene58769:CDS | 30.0% | |
| GAAAATAGTGAAGGAAATAG+AGG | - | chr8.3:9449711-9449730 | MS.gene58769:CDS | 30.0% | |
| TGCTCTTAATTTCAATAGTG+GGG | - | chr8.3:9449881-9449900 | MS.gene58769:CDS | 30.0% | |
| ! | ATGGGAAAAAACAAAAGCAT+AGG | - | chr8.3:9449835-9449854 | MS.gene58769:CDS | 30.0% |
| ! | TTAGGAAAATTAGTGGGTAT+GGG | - | chr8.3:9449817-9449836 | MS.gene58769:CDS | 30.0% |
| CTAAGTGGAAAACGTTTCTT+AGG | - | chr8.3:9449799-9449818 | MS.gene58769:CDS | 35.0% | |
| GAAAACCTTGTGGAAAAACT+TGG | + | chr8.3:9449936-9449955 | None:intergenic | 35.0% | |
| ! | CTAAGAAACGTTTTCCACTT+AGG | + | chr8.3:9449801-9449820 | None:intergenic | 35.0% |
| ! | CTTAGGAAAATTAGTGGGTA+TGG | - | chr8.3:9449816-9449835 | MS.gene58769:CDS | 35.0% |
| AACCTTGTGGAAAAACTTGG+AGG | + | chr8.3:9449933-9449952 | None:intergenic | 40.0% | |
| AAGATTTCGAAGAGACCAAC+GGG | - | chr8.3:9449604-9449623 | MS.gene58769:CDS | 40.0% | |
| CAAGATTTCGAAGAGACCAA+CGG | - | chr8.3:9449603-9449622 | MS.gene58769:CDS | 40.0% | |
| CTTTCCATTTCCATCACCAA+TGG | + | chr8.3:9449579-9449598 | None:intergenic | 40.0% | |
| GAAATAGAGGAGAAGAGACA+TGG | - | chr8.3:9449724-9449743 | MS.gene58769:CDS | 40.0% | |
| !! | AAGACCATTGGTGATGGAAA+TGG | - | chr8.3:9449572-9449591 | MS.gene58769:CDS | 40.0% |
| AAGTGATTGCTGGTCCTAAG+TGG | - | chr8.3:9449784-9449803 | MS.gene58769:CDS | 45.0% | |
| AGGAGAAGAGACATGGATGA+TGG | - | chr8.3:9449731-9449750 | MS.gene58769:CDS | 45.0% | |
| GAGACTTCAGAAGTGATTGC+TGG | - | chr8.3:9449774-9449793 | MS.gene58769:CDS | 45.0% | |
| ! | GGACAAGTTGAGGAAGATGA+AGG | - | chr8.3:9449752-9449771 | MS.gene58769:CDS | 45.0% |
| ! | TGCCTCCAAGTTTTTCCACA+AGG | - | chr8.3:9449928-9449947 | MS.gene58769:CDS | 45.0% |
| !! | CATGGATGATGGACAAGTTG+AGG | - | chr8.3:9449742-9449761 | MS.gene58769:CDS | 45.0% |
| CACCAATGGTCTTCCCTCAT+AGG | + | chr8.3:9449565-9449584 | None:intergenic | 50.0% | |
| GATGCTTACCTTCTTCGTCG+TGG | + | chr8.3:9449683-9449702 | None:intergenic | 50.0% | |
| TGAGGGAAGACCATTGGTGA+TGG | - | chr8.3:9449566-9449585 | MS.gene58769:CDS | 50.0% | |
| TGCCTATGAGGGAAGACCAT+TGG | - | chr8.3:9449560-9449579 | MS.gene58769:CDS | 50.0% | |
| TTCGAAGAGACCAACGGGTA+TGG | - | chr8.3:9449609-9449628 | MS.gene58769:CDS | 50.0% | |
| AGACCAACGGGTATGGATGC+GGG | - | chr8.3:9449616-9449635 | MS.gene58769:CDS | 55.0% | |
| GAGACCAACGGGTATGGATG+CGG | - | chr8.3:9449615-9449634 | MS.gene58769:CDS | 55.0% | |
| GTGGCGTTGCCACGATTTCA+AGG | + | chr8.3:9449664-9449683 | None:intergenic | 55.0% | |
| TCATTCTGGCGACGTGCATC+TGG | + | chr8.3:9449969-9449988 | None:intergenic | 55.0% | |
| TGGCGACGTGCATCTGGAAA+AGG | + | chr8.3:9449963-9449982 | None:intergenic | 55.0% | |
| AACGGGTATGGATGCGGGTG+CGG | - | chr8.3:9449621-9449640 | MS.gene58769:CDS | 60.0% | |
| TGGCAACGCCACGACGAAGA+AGG | - | chr8.3:9449672-9449691 | MS.gene58769:CDS | 60.0% | |
| GCACCCGCATCCATACCCGT+TGG | + | chr8.3:9449622-9449641 | None:intergenic | 65.0% | |
| GGTATGGATGCGGGTGCGGA+TGG | - | chr8.3:9449625-9449644 | MS.gene58769:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 9449555 | 9449995 | 9449555 | ID=MS.gene58769 |
| chr8.3 | mRNA | 9449555 | 9449995 | 9449555 | ID=MS.gene58769.t1;Parent=MS.gene58769 |
| chr8.3 | exon | 9449555 | 9449995 | 9449555 | ID=MS.gene58769.t1.exon1;Parent=MS.gene58769.t1 |
| chr8.3 | CDS | 9449555 | 9449995 | 9449555 | ID=cds.MS.gene58769.t1;Parent=MS.gene58769.t1 |
| Gene Sequence |
| Protein sequence |
