AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053206

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053206.t1 MTR_7g025610 65.158 221 40 3 116 299 368 588 3.45e-80 262
MS.gene053206.t1 MTR_7g025610 40.860 279 84 7 1 207 120 389 1.15e-44 166
MS.gene053206.t1 MTR_4g007150 67.204 186 41 3 208 392 19 185 2.29e-73 231
MS.gene053206.t1 MTR_7g025600 62.766 94 16 2 317 391 54 147 5.36e-28 109
MS.gene053206.t1 MTR_7g006590 33.721 172 84 4 22 186 4 152 5.77e-16 76.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053206.t1 AT1G09900 26.154 195 120 4 23 206 307 488 9.42e-13 70.9
MS.gene053206.t1 AT3G53700 28.144 167 87 3 23 156 293 459 4.29e-12 68.9

Find 84 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCATCAAACCGGTCTATTT+TGG 0.174032 4.2:+92493515 MS.gene053206:CDS
GTCCTAAAAGGTTTGGATTT+AGG 0.214804 4.2:+92492860 MS.gene053206:CDS
TTGGAATTTGTTGATTGATA+TGG 0.217593 4.2:+92492148 MS.gene053206:CDS
TCCATAGGCGTCTTAATTAA+AGG 0.235791 4.2:-92491333 None:intergenic
CAAAGGCCGAAAGTGTTATT+AGG 0.284034 4.2:+92493954 MS.gene053206:CDS
GTTGATTGATATGGGAGATT+TGG 0.296093 4.2:+92492157 MS.gene053206:CDS
TTGATTGATATGGGAGATTT+GGG 0.308902 4.2:+92492158 MS.gene053206:CDS
TGCGTAAATTTCAATGCAAT+TGG 0.310753 4.2:+92492129 MS.gene053206:CDS
CTTGAATCAATCATATCTTT+TGG 0.312627 4.2:-92492074 None:intergenic
CTAATAACACTTTCGGCCTT+TGG 0.313937 4.2:-92493953 None:intergenic
GAGAGAGTAAATGTCCTAAA+AGG 0.324760 4.2:+92492848 MS.gene053206:CDS
GGGGTATAAGTAACAGAATT+AGG 0.329322 4.2:-92491312 None:intergenic
CCAGACCATTGTTCTTCATC+TGG 0.335400 4.2:-92492008 None:intergenic
TCATCTGGTGATACAATGTT+CGG 0.337255 4.2:-92491993 None:intergenic
ATGTTGAAAGTGAGGAAATT+AGG 0.342683 4.2:-92490998 None:intergenic
TTGTAAATTCGGTAGGCTTA+AGG 0.347612 4.2:+92491245 MS.gene053206:intron
CCATAGGCGTCTTAATTAAA+GGG 0.352739 4.2:-92491332 None:intergenic
GATATCGAATGAGCTTAAAA+AGG 0.373317 4.2:+92491846 MS.gene053206:CDS
AGTAAATGTCCTAAAAGGTT+TGG 0.375306 4.2:+92492853 MS.gene053206:CDS
CTTATATTATTGTAGCCATA+TGG 0.375698 4.2:+92492806 MS.gene053206:intron
TAAGGCCTCATTCACCTTGT+TGG 0.381205 4.2:-92491358 None:intergenic
TAGAAGTGGAAGAACTTTAG+AGG 0.391431 4.2:+92491969 MS.gene053206:intron
TGGAATTTGTTGATTGATAT+GGG 0.396560 4.2:+92492149 MS.gene053206:CDS
GGCTTGATCTTTCTATGTGT+TGG 0.404381 4.2:-92493916 None:intergenic
GATCTTTCTATGTGTTGGTA+TGG 0.424563 4.2:-92493911 None:intergenic
AAAGGCTGGTCATTATCAAC+AGG 0.427342 4.2:+92491864 MS.gene053206:CDS
GCAACTGTTTAACCATTTGA+AGG 0.436003 4.2:-92492052 None:intergenic
AGTGAACCTAATAACACTTT+CGG 0.438434 4.2:-92493960 None:intergenic
ATGCTACACTCACTGAATAT+AGG 0.439534 4.2:+92493487 MS.gene053206:CDS
CCAGCAAAAGTATATTTATC+CGG 0.447772 4.2:-92491721 None:intergenic
AAAACCTTAAAAGCGTTCTT+CGG 0.450645 4.2:-92490959 None:intergenic
CATTCAGAACTGCGCTAAAG+TGG 0.451295 4.2:-92491113 None:intergenic
CAAAAGATATGATTGATTCA+AGG 0.455866 4.2:+92492075 MS.gene053206:CDS
CCCTTTAATTAAGACGCCTA+TGG 0.456363 4.2:+92491332 MS.gene053206:CDS
ATCTGAAATATCTTCAGAAC+TGG 0.456998 4.2:-92492264 None:intergenic
GCTCATTCGATATCACAGTA+TGG 0.457388 4.2:-92491837 None:intergenic
GGAGTAGGAGGCATGCGAGT+TGG 0.469507 4.2:+92492881 MS.gene053206:CDS
CATCTGGTGATACAATGTTC+GGG 0.473239 4.2:-92491992 None:intergenic
GGACATTTACTCTCTCAGAT+TGG 0.474452 4.2:-92492841 None:intergenic
TGAAGTAAAAGACCTTCAAA+TGG 0.489606 4.2:+92492040 MS.gene053206:CDS
TGCGCTAAAGTGGACGCGTT+AGG 0.492820 4.2:-92491103 None:intergenic
TAGCCATATGGATTTGATGT+AGG 0.493900 4.2:+92492818 MS.gene053206:intron
TCCTTTGCGACTGAATCTCC+TGG 0.495822 4.2:-92493572 None:intergenic
CAATGTGTTTCTTTGACGAA+AGG 0.496042 4.2:+92493547 MS.gene053206:CDS
GGTTTGGATTTAGGAGTAGG+AGG 0.498936 4.2:+92492869 MS.gene053206:CDS
AAAGGTTTGGATTTAGGAGT+AGG 0.499424 4.2:+92492866 MS.gene053206:CDS
CCAGATGAAGAACAATGGTC+TGG 0.502849 4.2:+92492008 MS.gene053206:CDS
GTAGGAGGCATGCGAGTTGG+AGG 0.503214 4.2:+92492884 MS.gene053206:CDS
TTTGGGTTTATTATCTTCCA+AGG 0.506364 4.2:+92492175 MS.gene053206:CDS
AGGCATGCGAGTTGGAGGCC+AGG 0.512087 4.2:+92492889 MS.gene053206:CDS
CCTTTGGTTTGTGACACGAC+AGG 0.520496 4.2:-92493937 None:intergenic
TGGCCTACATCAAATCCATA+TGG 0.522260 4.2:-92492821 None:intergenic
ATTCGATATCACAGTATGGA+TGG 0.522941 4.2:-92491833 None:intergenic
GATTTCTGAAGGTTTAAAGC+CGG 0.523099 4.2:+92491702 MS.gene053206:CDS
ACTTTCAGCATGCAACGCCT+TGG 0.525498 4.2:-92492192 None:intergenic
AATGCAGATAGAGTTTGCTC+AGG 0.527742 4.2:+92493343 MS.gene053206:CDS
TTTCTTTGACGAAAGGAACC+AGG 0.541643 4.2:+92493554 MS.gene053206:CDS
GGAATCCAACAAGGTGAATG+AGG 0.541802 4.2:+92491353 MS.gene053206:CDS
CTCAGGAAGTCGACATCACG+AGG 0.543884 4.2:+92493360 MS.gene053206:CDS
AAGTCCTTGCCAAAATAGAC+CGG 0.547409 4.2:-92493524 None:intergenic
TTCACCTTGTTGGATTCCAT+AGG 0.559983 4.2:-92491348 None:intergenic
CAATGTCACGGAAATTAGAA+AGG 0.564567 4.2:-92491033 None:intergenic
TTCGATATCACAGTATGGAT+GGG 0.574719 4.2:-92491832 None:intergenic
AATCCAACGTTGGAACTGAA+AGG 0.574876 4.2:+92494004 MS.gene053206:CDS
TACGAACGTATGATTTCTGA+AGG 0.575319 4.2:+92491691 MS.gene053206:CDS
CATAGGCGTCTTAATTAAAG+GGG 0.595822 4.2:-92491331 None:intergenic
ATGTTCTTAGAGAAATGTTG+AGG 0.595919 4.2:+92491068 MS.gene053206:CDS
ACCAGGAGATTCAGTCGCAA+AGG 0.605039 4.2:+92493571 MS.gene053206:CDS
GTTGAACAAGATTATACTGA+AGG 0.606144 4.2:+92493871 MS.gene053206:CDS
TTACCTTTCAGTTCCAACGT+TGG 0.612446 4.2:-92494007 None:intergenic
AAATCGTCGAGCTGTTAATG+AGG 0.613554 4.2:+92493601 MS.gene053206:CDS
GAGAGACATCAAAGTTCTCA+AGG 0.616542 4.2:+92493301 MS.gene053206:CDS
TGTCACGGAAATTAGAAAGG+TGG 0.623095 4.2:-92491030 None:intergenic
CATAGAATCTAATCCAACGT+TGG 0.624210 4.2:+92493994 MS.gene053206:CDS
ATGTAACATTGACAATGTCA+CGG 0.624741 4.2:-92491045 None:intergenic
AAAGCGCAATGTTGAAAGTG+AGG 0.626389 4.2:-92491006 None:intergenic
CATGTCATTAGAGAAACGTA+AGG 0.632409 4.2:-92491376 None:intergenic
AGTGTGTCGTGTTGTTACGA+TGG 0.636777 4.2:+92491792 MS.gene053206:CDS
GATATCACAGTATGGATGGG+AGG 0.657916 4.2:-92491829 None:intergenic
GACGCCTATGGAATCCAACA+AGG 0.667410 4.2:+92491344 MS.gene053206:CDS
CTATAGCAGAGGAATATCGA+AGG 0.675779 4.2:-92494044 None:intergenic
ACAATTGTTAGCTATAGCAG+AGG 0.676432 4.2:-92494055 None:intergenic
TATCACCAGATGAAGAACAA+TGG 0.690570 4.2:+92492003 MS.gene053206:CDS
CCTGTCGTGTCACAAACCAA+AGG 0.693419 4.2:+92493937 MS.gene053206:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAATTGAATGATTATA+TGG - chr4.2:92493094-92493113 None:intergenic 10.0%
!! AAATTGTTTAGATTTAGATT+TGG + chr4.2:92493188-92493207 MS.gene053206:intron 15.0%
!! ATATTAGAAAAAACTAAATC+AGG - chr4.2:92491420-92491439 None:intergenic 15.0%
!! CAATTATATTAATATCTCTT+TGG + chr4.2:92492649-92492668 MS.gene053206:intron 15.0%
!!! AATAATTTTTAAACATTCAG+AGG + chr4.2:92493652-92493671 MS.gene053206:intron 15.0%
!!! ATTCATCAATTTTGTAAATT+CGG + chr4.2:92491234-92491253 MS.gene053206:intron 15.0%
!! AAATCGTTACAATTAGTAAA+TGG + chr4.2:92492967-92492986 MS.gene053206:intron 20.0%
!! AACAATAAGACAAACAATAA+GGG - chr4.2:92492539-92492558 None:intergenic 20.0%
!! AATTCCTATATCTTTAAACT+CGG + chr4.2:92492358-92492377 MS.gene053206:intron 20.0%
!! ATAATTGAAAAGCAGATATA+TGG - chr4.2:92492636-92492655 None:intergenic 20.0%
!! TAACAATAAGACAAACAATA+AGG - chr4.2:92492540-92492559 None:intergenic 20.0%
!! TGAAGTAAATGTATACAAAT+CGG - chr4.2:92491529-92491548 None:intergenic 20.0%
!! TTTGTAAAATGAATGTGTTT+CGG + chr4.2:92491139-92491158 MS.gene053206:intron 20.0%
!!! AATCATTTTGTTGAACAAAT+CGG - chr4.2:92491277-92491296 None:intergenic 20.0%
!!! ACCTATACTTTTGATAAAAT+GGG + chr4.2:92493162-92493181 MS.gene053206:intron 20.0%
!!! ACCTTTGAAAAGAAAATATA+TGG - chr4.2:92492738-92492757 None:intergenic 20.0%
!!! TACTGAATTTTTTTTCTCTA+GGG - chr4.2:92493126-92493145 None:intergenic 20.0%
!!! TCCATATATTTTCTTTTCAA+AGG + chr4.2:92492734-92492753 MS.gene053206:intron 20.0%
!!! TTTGTTTTATGTTGAATTGT+AGG + chr4.2:92493825-92493844 MS.gene053206:intron 20.0%
! ACAATAAGACAAACAATAAG+GGG - chr4.2:92492538-92492557 None:intergenic 25.0%
! CAACAAGATTTATTATTAGC+TGG - chr4.2:92492481-92492500 None:intergenic 25.0%
! CTTATATTATTGTAGCCATA+TGG + chr4.2:92492806-92492825 MS.gene053206:intron 25.0%
! GAAGTAAATGTATACAAATC+GGG - chr4.2:92491528-92491547 None:intergenic 25.0%
! GTTTAGATTTAGATTTGGAT+TGG + chr4.2:92493193-92493212 MS.gene053206:intron 25.0%
! TAATGAGGTATACAAAAATC+TGG + chr4.2:92493616-92493635 MS.gene053206:intron 25.0%
! TTGAATTGTCTGTTAATGTT+TGG + chr4.2:92491202-92491221 MS.gene053206:intron 25.0%
!! ATCAATTTTGTAAATTCGGT+AGG + chr4.2:92491238-92491257 MS.gene053206:intron 25.0%
!! CAAAAGATATGATTGATTCA+AGG + chr4.2:92492075-92492094 MS.gene053206:CDS 25.0%
!! CACCTATACTTTTGATAAAA+TGG + chr4.2:92493161-92493180 MS.gene053206:intron 25.0%
!! CTTGAATCAATCATATCTTT+TGG - chr4.2:92492077-92492096 None:intergenic 25.0%
!!! ACCCATTTTATCAAAAGTAT+AGG - chr4.2:92493166-92493185 None:intergenic 25.0%
!!! ATTTGTATTTTTTTTCTGCG+AGG + chr4.2:92492434-92492453 MS.gene053206:intron 25.0%
!!! GTACTGAATTTTTTTTCTCT+AGG - chr4.2:92493127-92493146 None:intergenic 25.0%
!!! TGGAATTTGTTGATTGATAT+GGG + chr4.2:92492149-92492168 MS.gene053206:CDS 25.0%
!!! TTGGAATTTGTTGATTGATA+TGG + chr4.2:92492148-92492167 MS.gene053206:CDS 25.0%
!!! TTTTTTTCTCTAGGGTATAA+AGG - chr4.2:92493118-92493137 None:intergenic 25.0%
ACTAACGAAATTGAGGTTTA+TGG - chr4.2:92493229-92493248 None:intergenic 30.0%
ACTCAATAGTATGCACAAAT+AGG - chr4.2:92493703-92493722 None:intergenic 30.0%
AGAGATTAAACTGTTTAGAC+TGG - chr4.2:92491569-92491588 None:intergenic 30.0%
AGGATATTTCTTAAGTGCTT+TGG - chr4.2:92491908-92491927 None:intergenic 30.0%
AGTAAATGTCCTAAAAGGTT+TGG + chr4.2:92492853-92492872 MS.gene053206:CDS 30.0%
AGTGAACCTAATAACACTTT+CGG - chr4.2:92493963-92493982 None:intergenic 30.0%
ATCAATGCATATCTTAGAAG+TGG + chr4.2:92491955-92491974 MS.gene053206:intron 30.0%
ATCCTTTACATTTCATGTTG+CGG + chr4.2:92492563-92492582 MS.gene053206:intron 30.0%
ATGTAACATTGACAATGTCA+CGG - chr4.2:92491048-92491067 None:intergenic 30.0%
ATGTTCTTAGAGAAATGTTG+AGG + chr4.2:92491068-92491087 MS.gene053206:CDS 30.0%
ATGTTGAAAGTGAGGAAATT+AGG - chr4.2:92491001-92491020 None:intergenic 30.0%
ATTGATTGTCTTTCAAAGTC+CGG + chr4.2:92491444-92491463 MS.gene053206:intron 30.0%
ATTGCCGAGTTTAAAGATAT+AGG - chr4.2:92492365-92492384 None:intergenic 30.0%
CTCTTATTGTTACTATGTTG+TGG + chr4.2:92492309-92492328 MS.gene053206:intron 30.0%
GATATCGAATGAGCTTAAAA+AGG + chr4.2:92491846-92491865 MS.gene053206:CDS 30.0%
GCATAAAACTAACGAAATTG+AGG - chr4.2:92493236-92493255 None:intergenic 30.0%
GTTGAACAAGATTATACTGA+AGG + chr4.2:92493871-92493890 MS.gene053206:CDS 30.0%
TAGAGAATTTCAGTTGTTGT+TGG + chr4.2:92491485-92491504 MS.gene053206:intron 30.0%
TGAAGTAAAAGACCTTCAAA+TGG + chr4.2:92492040-92492059 MS.gene053206:CDS 30.0%
TGCGTAAATTTCAATGCAAT+TGG + chr4.2:92492129-92492148 MS.gene053206:CDS 30.0%
TGTCTACTTCTTTATTGAGA+AGG + chr4.2:92493425-92493444 MS.gene053206:intron 30.0%
TTGATTGATATGGGAGATTT+GGG + chr4.2:92492158-92492177 MS.gene053206:CDS 30.0%
! ATCTGAAATATCTTCAGAAC+TGG - chr4.2:92492267-92492286 None:intergenic 30.0%
! ATTTTTAAACATTCAGAGGC+AGG + chr4.2:92493656-92493675 MS.gene053206:intron 30.0%
! CCAGCAAAAGTATATTTATC+CGG - chr4.2:92491724-92491743 None:intergenic 30.0%
! CGGATAAATATACTTTTGCT+GGG + chr4.2:92491722-92491741 MS.gene053206:CDS 30.0%
! TCTTTTAATCTCCGATGTTA+AGG + chr4.2:92491647-92491666 MS.gene053206:intron 30.0%
! TTGTTGTTGATTGATTACTG+AGG + chr4.2:92492778-92492797 MS.gene053206:intron 30.0%
! TTTGGGTTTATTATCTTCCA+AGG + chr4.2:92492175-92492194 MS.gene053206:CDS 30.0%
!! AAAACCTTAAAAGCGTTCTT+CGG - chr4.2:92490962-92490981 None:intergenic 30.0%
!! ATATCTCTTTGGAATTCTTG+AGG + chr4.2:92492660-92492679 MS.gene053206:intron 30.0%
!! ATGAGATTGATTTTCACATG+AGG + chr4.2:92493787-92493806 MS.gene053206:intron 30.0%
!!! CTTTTAAGGTTTTCAGACTA+AGG + chr4.2:92490969-92490988 MS.gene053206:CDS 30.0%
AAAGGTTTGGATTTAGGAGT+AGG + chr4.2:92492866-92492885 MS.gene053206:CDS 35.0%
ATGCTACACTCACTGAATAT+AGG + chr4.2:92493487-92493506 MS.gene053206:CDS 35.0%
ATTCGATATCACAGTATGGA+TGG - chr4.2:92491836-92491855 None:intergenic 35.0%
CAATGTCACGGAAATTAGAA+AGG - chr4.2:92491036-92491055 None:intergenic 35.0%
CAATGTGTTTCTTTGACGAA+AGG + chr4.2:92493547-92493566 MS.gene053206:CDS 35.0%
CATAGAATCTAATCCAACGT+TGG + chr4.2:92493994-92494013 MS.gene053206:CDS 35.0%
CATAGGCGTCTTAATTAAAG+GGG - chr4.2:92491334-92491353 None:intergenic 35.0%
CATGTCATTAGAGAAACGTA+AGG - chr4.2:92491379-92491398 None:intergenic 35.0%
CCATAGGCGTCTTAATTAAA+GGG - chr4.2:92491335-92491354 None:intergenic 35.0%
GAGAGAGTAAATGTCCTAAA+AGG + chr4.2:92492848-92492867 MS.gene053206:CDS 35.0%
GCAACTGTTTAACCATTTGA+AGG - chr4.2:92492055-92492074 None:intergenic 35.0%
GGGGTATAAGTAACAGAATT+AGG - chr4.2:92491315-92491334 None:intergenic 35.0%
GTCCTAAAAGGTTTGGATTT+AGG + chr4.2:92492860-92492879 MS.gene053206:CDS 35.0%
GTTGATTGATATGGGAGATT+TGG + chr4.2:92492157-92492176 MS.gene053206:CDS 35.0%
TACGAACGTATGATTTCTGA+AGG + chr4.2:92491691-92491710 MS.gene053206:CDS 35.0%
TAGAAGTGGAAGAACTTTAG+AGG + chr4.2:92491969-92491988 MS.gene053206:intron 35.0%
TAGCCATATGGATTTGATGT+AGG + chr4.2:92492818-92492837 MS.gene053206:intron 35.0%
TATCACCAGATGAAGAACAA+TGG + chr4.2:92492003-92492022 MS.gene053206:CDS 35.0%
TCATCTGGTGATACAATGTT+CGG - chr4.2:92491996-92492015 None:intergenic 35.0%
TCCATAGGCGTCTTAATTAA+AGG - chr4.2:92491336-92491355 None:intergenic 35.0%
TCTAATGACATGAAGTCTGT+TGG + chr4.2:92491387-92491406 MS.gene053206:intron 35.0%
TCTTCACTTGCGATACTAAT+CGG + chr4.2:92492995-92493014 MS.gene053206:intron 35.0%
TGAACCTTTCAATCAGATGT+CGG - chr4.2:92492335-92492354 None:intergenic 35.0%
TTCGATATCACAGTATGGAT+GGG - chr4.2:92491835-92491854 None:intergenic 35.0%
TTGTAAATTCGGTAGGCTTA+AGG + chr4.2:92491245-92491264 MS.gene053206:intron 35.0%
! AGTGAGTGTAGCATACTTTT+TGG - chr4.2:92493481-92493500 None:intergenic 35.0%
! ATAGGAGTTTTGCATCAAAC+CGG + chr4.2:92493505-92493524 MS.gene053206:CDS 35.0%
! CCGGATAAATATACTTTTGC+TGG + chr4.2:92491721-92491740 MS.gene053206:CDS 35.0%
! CGGCAAGTTTATCAGATTTT+GGG + chr4.2:92491159-92491178 MS.gene053206:intron 35.0%
! CTCCTAAATCCAAACCTTTT+AGG - chr4.2:92492865-92492884 None:intergenic 35.0%
! GATCTTTCTATGTGTTGGTA+TGG - chr4.2:92493914-92493933 None:intergenic 35.0%
! GATTTCTGAAGGTTTAAAGC+CGG + chr4.2:92491702-92491721 MS.gene053206:CDS 35.0%
! TCGGCAAGTTTATCAGATTT+TGG + chr4.2:92491158-92491177 MS.gene053206:intron 35.0%
!!! GTATTTTTTTTCTGCGAGGA+GGG + chr4.2:92492438-92492457 MS.gene053206:intron 35.0%
!!! TGTATTTTTTTTCTGCGAGG+AGG + chr4.2:92492437-92492456 MS.gene053206:intron 35.0%
AAAGCGCAATGTTGAAAGTG+AGG - chr4.2:92491009-92491028 None:intergenic 40.0%
AAATCGTCGAGCTGTTAATG+AGG + chr4.2:92493601-92493620 MS.gene053206:CDS 40.0%
AAGTCCTTGCCAAAATAGAC+CGG - chr4.2:92493527-92493546 None:intergenic 40.0%
AATCCAACGTTGGAACTGAA+AGG + chr4.2:92494004-92494023 MS.gene053206:CDS 40.0%
AATCGGCAACTGCATTGAAT+TGG + chr4.2:92493012-92493031 MS.gene053206:intron 40.0%
AGAAGTAGACAGACTTCAGA+TGG - chr4.2:92493416-92493435 None:intergenic 40.0%
AGGTCGAGAGGCTAATAATA+AGG + chr4.2:92492680-92492699 MS.gene053206:intron 40.0%
ATCTATCCACAAACTGGTAC+TGG + chr4.2:92492383-92492402 MS.gene053206:intron 40.0%
CACCGCAACATGAAATGTAA+AGG - chr4.2:92492568-92492587 None:intergenic 40.0%
CATCTGGTGATACAATGTTC+GGG - chr4.2:92491995-92492014 None:intergenic 40.0%
CCCTTTAATTAAGACGCCTA+TGG + chr4.2:92491332-92491351 MS.gene053206:CDS 40.0%
CTAATAACACTTTCGGCCTT+TGG - chr4.2:92493956-92493975 None:intergenic 40.0%
CTATAGCAGAGGAATATCGA+AGG - chr4.2:92494047-92494066 None:intergenic 40.0%
CTTTACATTTCATGTTGCGG+TGG + chr4.2:92492566-92492585 MS.gene053206:intron 40.0%
GAGAGACATCAAAGTTCTCA+AGG + chr4.2:92493301-92493320 MS.gene053206:CDS 40.0%
GCACAAATAGGAACTCTAGT+AGG - chr4.2:92493691-92493710 None:intergenic 40.0%
GCTCATTCGATATCACAGTA+TGG - chr4.2:92491840-92491859 None:intergenic 40.0%
GGACATTTACTCTCTCAGAT+TGG - chr4.2:92492844-92492863 None:intergenic 40.0%
GGCTTGATCTTTCTATGTGT+TGG - chr4.2:92493919-92493938 None:intergenic 40.0%
TAATCTCCGATGTTAAGGCT+GGG + chr4.2:92491652-92491671 MS.gene053206:intron 40.0%
TCGAATGAGCTTAAAAAGGC+TGG + chr4.2:92491850-92491869 MS.gene053206:CDS 40.0%
TGATAACCAGTACCAGTTTG+TGG - chr4.2:92492392-92492411 None:intergenic 40.0%
TGGCCTACATCAAATCCATA+TGG - chr4.2:92492824-92492843 None:intergenic 40.0%
TGGGTGTAATGCTTGGTTTA+AGG + chr4.2:92491178-92491197 MS.gene053206:intron 40.0%
TGTCACGGAAATTAGAAAGG+TGG - chr4.2:92491033-92491052 None:intergenic 40.0%
TTAATCTCCGATGTTAAGGC+TGG + chr4.2:92491651-92491670 MS.gene053206:intron 40.0%
TTACCTTTCAGTTCCAACGT+TGG - chr4.2:92494010-92494029 None:intergenic 40.0%
TTCACCTTGTTGGATTCCAT+AGG - chr4.2:92491351-92491370 None:intergenic 40.0%
TTTCTTTGACGAAAGGAACC+AGG + chr4.2:92493554-92493573 MS.gene053206:CDS 40.0%
! AAAGGCTGGTCATTATCAAC+AGG + chr4.2:92491864-92491883 MS.gene053206:CDS 40.0%
! AATGCAGATAGAGTTTGCTC+AGG + chr4.2:92493343-92493362 MS.gene053206:CDS 40.0%
! TCAACCGAAGAACGCTTTTA+AGG + chr4.2:92490955-92490974 MS.gene053206:CDS 40.0%
! TGCATCAAACCGGTCTATTT+TGG + chr4.2:92493515-92493534 MS.gene053206:CDS 40.0%
! TTTTCAGCACAAAGTGCACT+TGG - chr4.2:92491751-92491770 None:intergenic 40.0%
!! CAAAGGCCGAAAGTGTTATT+AGG + chr4.2:92493954-92493973 MS.gene053206:CDS 40.0%
!! CAGATTTTGGGTGTAATGCT+TGG + chr4.2:92491171-92491190 MS.gene053206:intron 40.0%
!! GTGTAGCATACTTTTTGGCA+TGG - chr4.2:92493476-92493495 None:intergenic 40.0%
ACAATAAGGGGTAGCAACAC+AGG - chr4.2:92492526-92492545 None:intergenic 45.0%
CATTCAGAACTGCGCTAAAG+TGG - chr4.2:92491116-92491135 None:intergenic 45.0%
CCAGACCATTGTTCTTCATC+TGG - chr4.2:92492011-92492030 None:intergenic 45.0%
CCAGATGAAGAACAATGGTC+TGG + chr4.2:92492008-92492027 MS.gene053206:CDS 45.0%
CCATATGCTACACTGTCAAG+AGG - chr4.2:92491928-92491947 None:intergenic 45.0%
CCTCTTGACAGTGTAGCATA+TGG + chr4.2:92491925-92491944 MS.gene053206:intron 45.0%
GACAGTGTAGCATATGGTGT+TGG + chr4.2:92491931-92491950 MS.gene053206:intron 45.0%
GATATCACAGTATGGATGGG+AGG - chr4.2:92491832-92491851 None:intergenic 45.0%
GGAATCCAACAAGGTGAATG+AGG + chr4.2:92491353-92491372 MS.gene053206:CDS 45.0%
GGTTTGGATTTAGGAGTAGG+AGG + chr4.2:92492869-92492888 MS.gene053206:CDS 45.0%
GTTAATTGTCCCTTCTGAGC+TGG + chr4.2:92493263-92493282 MS.gene053206:intron 45.0%
TAAGGCCTCATTCACCTTGT+TGG - chr4.2:92491361-92491380 None:intergenic 45.0%
TCGGCAATCTATCCACAAAC+TGG + chr4.2:92492377-92492396 MS.gene053206:intron 45.0%
TGCTAGAATCACTTCTCACC+TGG - chr4.2:92492910-92492929 None:intergenic 45.0%
TTGGAATTCTTGAGGTCGAG+AGG + chr4.2:92492668-92492687 MS.gene053206:intron 45.0%
! AGTGTGTCGTGTTGTTACGA+TGG + chr4.2:92491792-92491811 MS.gene053206:CDS 45.0%
! TCGCAGCGTTGAACTAATTC+AGG - chr4.2:92491594-92491613 None:intergenic 45.0%
! TTCAAAGTCCGGAAAGCATG+AGG + chr4.2:92491455-92491474 MS.gene053206:intron 45.0%
!!! CAAACCGGTCTATTTTGGCA+AGG + chr4.2:92493520-92493539 MS.gene053206:CDS 45.0%
ACCAGGAGATTCAGTCGCAA+AGG + chr4.2:92493571-92493590 MS.gene053206:CDS 50.0%
ACTTTCAGCATGCAACGCCT+TGG - chr4.2:92492195-92492214 None:intergenic 50.0%
CCTGTCGTGTCACAAACCAA+AGG + chr4.2:92493937-92493956 MS.gene053206:CDS 50.0%
GACGCCTATGGAATCCAACA+AGG + chr4.2:92491344-92491363 MS.gene053206:CDS 50.0%
GCACTACCCAGCCTTAACAT+CGG - chr4.2:92491661-92491680 None:intergenic 50.0%
GGGGTAGCAACACAGGATAT+AGG - chr4.2:92492519-92492538 None:intergenic 50.0%
GTGGCCGACATCTGATTGAA+AGG + chr4.2:92492328-92492347 MS.gene053206:intron 50.0%
TCCTTTGCGACTGAATCTCC+TGG - chr4.2:92493575-92493594 None:intergenic 50.0%
TGTCTCTCCCTTGCTTCCAT+AGG - chr4.2:92493289-92493308 None:intergenic 50.0%
TTCCATAGGCCAGCTCAGAA+GGG - chr4.2:92493275-92493294 None:intergenic 50.0%
! AAAGTCCGGAAAGCATGAGG+AGG + chr4.2:92491458-92491477 MS.gene053206:intron 50.0%
!! CCTTTGGTTTGTGACACGAC+AGG - chr4.2:92493940-92493959 None:intergenic 50.0%
AACCGCCTCCTCATGCTTTC+CGG - chr4.2:92491466-92491485 None:intergenic 55.0%
CTCAGGAAGTCGACATCACG+AGG + chr4.2:92493360-92493379 MS.gene053206:CDS 55.0%
CTTCCATAGGCCAGCTCAGA+AGG - chr4.2:92493276-92493295 None:intergenic 55.0%
TGCGCTAAAGTGGACGCGTT+AGG - chr4.2:92491106-92491125 None:intergenic 55.0%
! GAGCTGGCCTATGGAAGCAA+GGG + chr4.2:92493279-92493298 MS.gene053206:intron 55.0%
! TGAGCTGGCCTATGGAAGCA+AGG + chr4.2:92493278-92493297 MS.gene053206:intron 55.0%
! GGAGTAGGAGGCATGCGAGT+TGG + chr4.2:92492881-92492900 MS.gene053206:CDS 60.0%
! GTAGGAGGCATGCGAGTTGG+AGG + chr4.2:92492884-92492903 MS.gene053206:CDS 60.0%
! GTCCCTTCTGAGCTGGCCTA+TGG + chr4.2:92493270-92493289 MS.gene053206:intron 60.0%
! GTCCGGAAAGCATGAGGAGG+CGG + chr4.2:92491461-92491480 MS.gene053206:intron 60.0%
! AGGCATGCGAGTTGGAGGCC+AGG + chr4.2:92492889-92492908 MS.gene053206:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 92490932 92494066 92490932 ID=MS.gene053206
chr4.2 mRNA 92490932 92494066 92490932 ID=MS.gene053206.t1;Parent=MS.gene053206
chr4.2 exon 92490932 92491125 92490932 ID=MS.gene053206.t1.exon1;Parent=MS.gene053206.t1
chr4.2 CDS 92490932 92491125 92490932 ID=cds.MS.gene053206.t1;Parent=MS.gene053206.t1
chr4.2 exon 92491260 92491400 92491260 ID=MS.gene053206.t1.exon2;Parent=MS.gene053206.t1
chr4.2 CDS 92491260 92491400 92491260 ID=cds.MS.gene053206.t1;Parent=MS.gene053206.t1
chr4.2 exon 92491669 92491894 92491669 ID=MS.gene053206.t1.exon3;Parent=MS.gene053206.t1
chr4.2 CDS 92491669 92491894 92491669 ID=cds.MS.gene053206.t1;Parent=MS.gene053206.t1
chr4.2 exon 92491972 92492298 92491972 ID=MS.gene053206.t1.exon4;Parent=MS.gene053206.t1
chr4.2 CDS 92491972 92492298 92491972 ID=cds.MS.gene053206.t1;Parent=MS.gene053206.t1
chr4.2 exon 92492821 92492910 92492821 ID=MS.gene053206.t1.exon5;Parent=MS.gene053206.t1
chr4.2 CDS 92492821 92492910 92492821 ID=cds.MS.gene053206.t1;Parent=MS.gene053206.t1
chr4.2 exon 92493296 92493381 92493296 ID=MS.gene053206.t1.exon6;Parent=MS.gene053206.t1
chr4.2 CDS 92493296 92493381 92493296 ID=cds.MS.gene053206.t1;Parent=MS.gene053206.t1
chr4.2 exon 92493464 92493622 92493464 ID=MS.gene053206.t1.exon7;Parent=MS.gene053206.t1
chr4.2 CDS 92493464 92493622 92493464 ID=cds.MS.gene053206.t1;Parent=MS.gene053206.t1
chr4.2 exon 92493847 92494066 92493847 ID=MS.gene053206.t1.exon8;Parent=MS.gene053206.t1
chr4.2 CDS 92493847 92494066 92493847 ID=cds.MS.gene053206.t1;Parent=MS.gene053206.t1
Gene Sequence

>MS.gene053206

ATGGAATCTCTGTTTAAAGCTTCTCAACCGAAGAACGCTTTTAAGGTTTTCAGACTAAGGAAATCACCTAATTTCCTCACTTTCAACATTGCGCTTTTCCACCTTTCTAATTTCCGTGACATTGTCAATGTTACATATGTTCTTAGAGAAATGTTGAGGTTGCGTTATTATCCTAACGCGTCCACTTTAGCGCAGTTCTGAATGTGTTTTGTAAAATGAATGTGTTTCGGCAAGTTTATCAGATTTTGGGTGTAATGCTTGGTTTAAGGATTGAATTGTCTGTTAATGTTTGGACTGTACTTATTCATCAATTTTGTAAATTCGGTAGGCTTAAGGTTGCTACCGATTTGTTCAACAAAATGATTCGAAGCAATTGTTCTCCTAATTCTGTTACTTATACCCCTTTAATTAAGACGCCTATGGAATCCAACAAGGTGAATGAGGCCTTACGTTTCTCTAATGACATGAAGTCTGTTGGTATAGTTCCTGATTTAGTTTTTTCTAATATGTTAATTGATTGTCTTTCAAAGTCCGGAAAGCATGAGGAGGCGGTTAGAGAATTTCAGTTGTTGTTGGAGCAAAAAAACTTTAAACCCGATTTGTATACATTTACTTCATTGTTGTCTGTAATCTGCCAGTCTAAACAGTTTAATCTCTTACCTGAATTAGTTCAACGCTGCGAACATATACGTCGTGATTTAGTGTTCTGCAATGCTCTTTTAATCTCCGATGTTAAGGCTGGGTAGTGCTCTTGAACTCTACGAACGTATGATTTCTGAAGGTTTAAAGCCGGATAAATATACTTTTGCTGGGCTACCAAGTGCACTTTGTGCTGAAAACAGAAATGATGAAGCAGTTAAAGTGTGTCGTGTTGTTACGATGGATCACGCGAATGATCCTCCCATCCATACTGTGATATCGAATGAGCTTAAAAAGGCTGGTCATTATCAACAGGCTTCTGATGTTTTTATATCCAAAGCACTTAAGAAATATCCTCTTGACAGTGTAGCATATGGTGTTGGCATCAATGCATATCTTAGAAGTGGAAGAACTTTAGAGGCCCGAACATTGTATCACCAGATGAAGAACAATGGTCTGGAAGTTCATTTGAAGTAAAAGACCTTCAAATGGTTAAACAGTTGCCAAAAGATATGATTGATTCAAGGATCAAGTTGAGTGACAGAAATTTCTTCAACTTGCGTAAATTTCAATGCAATTGGAATTTGTTGATTGATATGGGAGATTTGGGTTTATTATCTTCCAAGGCGTTGCATGCTGAAAGTGTAAATGCAAATTATAAACATTGTGCTGAAGTTGATACAGAATGCAACGCCAGTTCTGAAGATATTTCAGATGTAGCTGTGTCAGTTTGTTGAGCTCTTATTGTTACTATGTTGTGGCCGACATCTGATTGAAAGGTTCATTCAATTCCTATATCTTTAAACTCGGCAATCTATCCACAAACTGGTACTGGTTATCACTTATCACGTCTTATATTCTTAATTTGTATTTTTTTTCTGCGAGGAGGGATAAATATTATTATTTTCCAGCTAATAATAAATCTTGTTGTAGTGCTTGTGTCTTCCTATATCCTGTGTTGCTACCCCTTATTGTTTGTCTTATTGTTACTTATCCTTTACATTTCATGTTGCGGTGGATCTGAAGATTTTTGACTCATCTCTTCTATAACACTAGTACTTTCCATATATCTGCTTTTCAATTATATTAATATCTCTTTGGAATTCTTGAGGTCGAGAGGCTAATAATAAGGACAAACTCTGATAAATATAACACTAGTACTTTCCATATATTTTCTTTTCAAAGGTAGTCTCAATTAACTAAAATTTTGTTGTTGATTGATTACTGAGGACATTCTTATATTATTGTAGCCATATGGATTTGATGTAGGCCAATCTGAGAGAGTAAATGTCCTAAAAGGTTTGGATTTAGGAGTAGGAGGCATGCGAGTTGGAGGCCAGGTGAGAAGTGATTCTAGCAGCAAGTATATCTATCTGTTCTGTTACAAATTTAATTGAAATCGTTACAATTAGTAAATGGAAGCTTCTTCACTTGCGATACTAATCGGCAACTGCATTGAATTGGAACTTATCTTACTCAAGATTTTTGTTTTGTTTTACTTCATTTCGTTTAGTTAACTGCCATATAATCATTCAATTATTTTACCTTTATACCCTAGAGAAAAAAAATTCAGTACACTATTAGCATTTGCACCTATACTTTTGATAAAATGGGTGAAAATTGTTTAGATTTAGATTTGGATTGGATAATATGCTCCATAAACCTCAATTTCGTTAGTTTTATGCAGAAACTGTTAATTGTCCCTTCTGAGCTGGCCTATGGAAGCAAGGGAGAGACATCAAAGTTCTCAAGGCTGAGCATGTAAGTTCAGAAATGCAGATAGAGTTTGCTCAGGAAGTCGACATCACGAGGTTTGTTGTTTTAAATTTTCTCTTTTTTTTACCATCTGAAGTCTGTCTACTTCTTTATTGAGAAGGTAATTTTGCATGACAGAACTGTTAACCATGCCAAAAAGTATGCTACACTCACTGAATATAGGAGTTTTGCATCAAACCGGTCTATTTTGGCAAGGACTTCAATGTGTTTCTTTGACGAAAGGAACCAGGAGATTCAGTCGCAAAGGAATATGAAAATCGTCGAGCTGTTAATGAGGTATACAAAAATCTGGTTATTGTTTTTTTAATAATTTTTAAACATTCAGAGGCAGGTTATAGTTGCCTACTAGAGTTCCTATTTGTGCATACTATTGAGTTAGATATTTGCTTTTATGCTTGCTTTTTAGTTGTGCTGAACAAGATTGTATTTCAGATGTACAGATGAGATTGATTTTCACATGAGGCATTAATTTATCTTGTTTGTTTTATGTTGAATTGTAGGATGTGTCTTCTGATGTGTTATACGTTGAACAAGATTATACTGAAGGAGTTGAACTATATTAGACCATACCAACACATAGAAAGATCAAGCCTGTCGTGTCACAAACCAAAGGCCGAAAGTGTTATTAGGTTCACTGTTGTTGTCATCATAGAATCTAATCCAACGTTGGAACTGAAAGGTAAATTTACTCGTTTCACCTTCGATATTCCTCTGCTATAG
Protein sequence

>MS.gene053206.t1

MESLFKASQPKNAFKVFRLRKSPNFLTFNIALFHLSNFRDIVNVTYVLREMLRLRYYPNASTLAQLKVATDLFNKMIRSNCSPNSVTYTPLIKTPMESNKVNEALRFSNDMKLGSALELYERMISEGLKPDKYTFAGLPSALCAENRNDEAVKVCRVVTMDHANDPPIHTVISNELKKAGHYQQASDKWKNFRGPNIVSPDEEQWSGSSFEVKDLQMVKQLPKDMIDSRIKLSDRNFFNLRKFQCNWNLLIDMGDLGLLSSKALHAESVNANYKHCAEVDTECNASSEDISDVAVSPYGFDVGQSERVNVLKGLDLGVGGMRVGGQQGRDIKVLKAEHVSSEMQIEFAQEVDITRTVNHAKKYATLTEYRSFASNRSILARTSMCFFDERNQEIQSQRNMKIVELLMRMCLLMCYTLNKIILKELNYIRPYQHIERSSLSCHKPKAESVIRFTVVVIIESNPTLELKGKFTRFTFDIPLL