Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053875.t1 | GAU44054.1 | 62.1 | 87 | 31 | 1 | 1 | 85 | 166 | 252 | 2.20E-21 | 111.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053875.t1 | Q9LVU8 | 50.0 | 82 | 39 | 1 | 1 | 80 | 168 | 249 | 4.1e-16 | 85.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053875.t1 | A0A2Z6NHY1 | 62.1 | 87 | 31 | 1 | 1 | 85 | 166 | 252 | 1.6e-21 | 111.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene053875.t1 | TF | RWP-RK |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053875 | MS.gene24211 | 0.800474 | 1.42E-48 | -1.69E-46 |
| MS.gene053875 | MS.gene27720 | 0.802427 | 5.64E-49 | -1.69E-46 |
| MS.gene053875 | MS.gene62748 | 0.803738 | 3.02E-49 | -1.69E-46 |
| MS.gene053875 | MS.gene73540 | 0.823455 | 1.37E-53 | -1.69E-46 |
| MS.gene053875 | MS.gene76389 | 0.802937 | 4.43E-49 | -1.69E-46 |
| MS.gene053875 | MS.gene79824 | 0.803799 | 2.93E-49 | -1.69E-46 |
| MS.gene053875 | MS.gene81193 | 0.803236 | 3.84E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053875.t1 | MTR_3g091700 | 61.628 | 86 | 31 | 1 | 1 | 84 | 174 | 259 | 5.67e-30 | 107 |
| MS.gene053875.t1 | MTR_3g435550 | 43.820 | 89 | 42 | 2 | 1 | 81 | 79 | 167 | 3.61e-17 | 72.4 |
| MS.gene053875.t1 | MTR_5g024070 | 34.783 | 92 | 47 | 1 | 1 | 79 | 221 | 312 | 5.74e-12 | 59.7 |
| MS.gene053875.t1 | MTR_0140s0010 | 36.471 | 85 | 43 | 2 | 11 | 84 | 150 | 234 | 3.05e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053875.t1 | AT5G53040 | 50.000 | 82 | 39 | 1 | 1 | 80 | 168 | 249 | 9.40e-22 | 85.5 |
| MS.gene053875.t1 | AT5G66990 | 41.667 | 96 | 43 | 2 | 1 | 83 | 179 | 274 | 3.51e-17 | 73.6 |
| MS.gene053875.t1 | AT1G74480 | 40.476 | 84 | 40 | 1 | 1 | 74 | 155 | 238 | 1.47e-15 | 69.7 |
| MS.gene053875.t1 | AT1G18790 | 42.391 | 92 | 41 | 3 | 1 | 80 | 148 | 239 | 1.85e-15 | 68.9 |
| MS.gene053875.t1 | AT4G35590 | 36.458 | 96 | 46 | 2 | 1 | 83 | 262 | 355 | 5.54e-11 | 57.0 |
| MS.gene053875.t1 | AT4G35590 | 36.458 | 96 | 46 | 2 | 1 | 83 | 212 | 305 | 6.77e-11 | 56.6 |
Find 17 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCACTCATAAACGACTTTA+AGG | 0.218317 | 5.3:+43871303 | MS.gene053875:CDS |
| GTTTGTTTGATACAGGAATT+AGG | 0.292311 | 5.3:+43871694 | MS.gene053875:intron |
| GAATGTTGGGTTGACCCTTT+TGG | 0.340960 | 5.3:+43871219 | MS.gene053875:CDS |
| TTTGATACAGGAATTAGGTT+TGG | 0.375313 | 5.3:+43871699 | MS.gene053875:intron |
| AAGATGCTGGAGGAATTTCC+TGG | 0.410842 | 5.3:+43871754 | MS.gene053875:CDS |
| CTGCCAAGAATATGAATGTT+GGG | 0.520209 | 5.3:+43871206 | None:intergenic |
| ACGATGAGTTCGAGAAACTA+AGG | 0.525507 | 5.3:+43871788 | MS.gene053875:CDS |
| TCATCGTTCAATTCCATCCC+AGG | 0.546218 | 5.3:-43871772 | None:intergenic |
| GTGAAGAAGTTCAACGATGG+AGG | 0.550347 | 5.3:-43871849 | None:intergenic |
| AGTCGTTTATGAGTGACCTC+AGG | 0.551618 | 5.3:-43871297 | None:intergenic |
| GCTGGAGGAATTTCCTGGGA+TGG | 0.564591 | 5.3:+43871759 | MS.gene053875:CDS |
| AGATGCTGGAGGAATTTCCT+GGG | 0.585900 | 5.3:+43871755 | MS.gene053875:CDS |
| GCTGCCAAGAATATGAATGT+TGG | 0.589287 | 5.3:+43871205 | None:intergenic |
| ATAGAAAATTTAAGAGCCTG+AGG | 0.606382 | 5.3:+43871281 | MS.gene053875:CDS |
| TGAAGAAGTTCAACGATGGA+GGG | 0.613260 | 5.3:-43871848 | None:intergenic |
| AGGAGTTCAACATAACAAAG+TGG | 0.670723 | 5.3:+43871254 | MS.gene053875:CDS |
| ATGGTGAAGAAGTTCAACGA+TGG | 0.684820 | 5.3:-43871852 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTAATCTTAAATGATCAA+TGG | + | chr5.3:43871654-43871673 | MS.gene053875:intron | 15.0% |
| !! | GCTTAAAAGTTAAAAATGTT+AGG | - | chr5.3:43871361-43871380 | None:intergenic | 20.0% |
| !!! | ATTCTTATGTTGTTTGATTA+TGG | + | chr5.3:43871329-43871348 | MS.gene053875:intron | 20.0% |
| ! | CCAAATTAAAGACTATTAGT+AGG | + | chr5.3:43871620-43871639 | MS.gene053875:intron | 25.0% |
| ! | CCTACTAATAGTCTTTAATT+TGG | - | chr5.3:43871623-43871642 | None:intergenic | 25.0% |
| ! | TCAATTCACAACAAAATAGA+TGG | - | chr5.3:43871403-43871422 | None:intergenic | 25.0% |
| !! | AGGCTCTTAAATTTTCTATA+AGG | - | chr5.3:43871280-43871299 | None:intergenic | 25.0% |
| !! | ATCAATGGTTTCAAAGTATT+TGG | + | chr5.3:43871669-43871688 | MS.gene053875:intron | 25.0% |
| !! | ATGCCATAATTGTTTTCAAT+TGG | + | chr5.3:43871484-43871503 | MS.gene053875:intron | 25.0% |
| ATAGAAAATTTAAGAGCCTG+AGG | + | chr5.3:43871281-43871300 | MS.gene053875:CDS | 30.0% | |
| CTACAAAGAAAAAATGAGCT+AGG | - | chr5.3:43871551-43871570 | None:intergenic | 30.0% | |
| GTTTGTTTGATACAGGAATT+AGG | + | chr5.3:43871694-43871713 | MS.gene053875:intron | 30.0% | |
| TGTTGTGAATTGAGCAATTT+AGG | + | chr5.3:43871410-43871429 | MS.gene053875:intron | 30.0% | |
| TTTGATACAGGAATTAGGTT+TGG | + | chr5.3:43871699-43871718 | MS.gene053875:intron | 30.0% | |
| TTTGGTTGTTTGTTTGATAC+AGG | + | chr5.3:43871687-43871706 | MS.gene053875:intron | 30.0% | |
| !!! | TAGCATTTTGAGTTATGGAA+TGG | + | chr5.3:43871594-43871613 | MS.gene053875:intron | 30.0% |
| !!! | TTCTGTAGCATTTTGAGTTA+TGG | + | chr5.3:43871589-43871608 | MS.gene053875:intron | 30.0% |
| AGGAGTTCAACATAACAAAG+TGG | + | chr5.3:43871254-43871273 | MS.gene053875:CDS | 35.0% | |
| GCGCCAATTGAAAACAATTA+TGG | - | chr5.3:43871490-43871509 | None:intergenic | 35.0% | |
| GGAATTAGGTTTGGAAAAAG+AGG | + | chr5.3:43871708-43871727 | MS.gene053875:intron | 35.0% | |
| GTCACTCATAAACGACTTTA+AGG | + | chr5.3:43871303-43871322 | MS.gene053875:CDS | 35.0% | |
| ACGATGAGTTCGAGAAACTA+AGG | + | chr5.3:43871788-43871807 | MS.gene053875:CDS | 40.0% | |
| ATCTTGTACCAGTGCTTACT+TGG | + | chr5.3:43871510-43871529 | MS.gene053875:intron | 40.0% | |
| GAAGCAAAAAAAGATGCTGG+AGG | + | chr5.3:43871744-43871763 | MS.gene053875:CDS | 40.0% | |
| GGAGAAGCAAAAAAAGATGC+TGG | + | chr5.3:43871741-43871760 | MS.gene053875:CDS | 40.0% | |
| !!! | AATGTTGGGTTGACCCTTTT+GGG | + | chr5.3:43871220-43871239 | MS.gene053875:CDS | 40.0% |
| AAGATGCTGGAGGAATTTCC+TGG | + | chr5.3:43871754-43871773 | MS.gene053875:CDS | 45.0% | |
| AGATGCTGGAGGAATTTCCT+GGG | + | chr5.3:43871755-43871774 | MS.gene053875:CDS | 45.0% | |
| AGTCGTTTATGAGTGACCTC+AGG | - | chr5.3:43871300-43871319 | None:intergenic | 45.0% | |
| GGAAAAAGAGGTTGCTACGT+TGG | + | chr5.3:43871720-43871739 | MS.gene053875:CDS | 45.0% | |
| TACCAGTGCTTACTTGGTAG+CGG | + | chr5.3:43871516-43871535 | MS.gene053875:intron | 45.0% | |
| TCATCGTTCAATTCCATCCC+AGG | - | chr5.3:43871775-43871794 | None:intergenic | 45.0% | |
| ! | CTTGCACCTTTTCCCCAAAA+GGG | - | chr5.3:43871236-43871255 | None:intergenic | 45.0% |
| !!! | ATGTTGGGTTGACCCTTTTG+GGG | + | chr5.3:43871221-43871240 | MS.gene053875:CDS | 45.0% |
| !!! | GAATGTTGGGTTGACCCTTT+TGG | + | chr5.3:43871219-43871238 | MS.gene053875:CDS | 45.0% |
| ATCCGCTACCAAGTAAGCAC+TGG | - | chr5.3:43871521-43871540 | None:intergenic | 50.0% | |
| ! | CCTTGCACCTTTTCCCCAAA+AGG | - | chr5.3:43871237-43871256 | None:intergenic | 50.0% |
| !! | CCTTTTGGGGAAAAGGTGCA+AGG | + | chr5.3:43871234-43871253 | MS.gene053875:CDS | 50.0% |
| !! | GGTTGACCCTTTTGGGGAAA+AGG | + | chr5.3:43871227-43871246 | MS.gene053875:CDS | 50.0% |
| GCTGGAGGAATTTCCTGGGA+TGG | + | chr5.3:43871759-43871778 | MS.gene053875:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 43871217 | 43871861 | 43871217 | ID=MS.gene053875 |
| chr5.3 | mRNA | 43871217 | 43871861 | 43871217 | ID=MS.gene053875.t1;Parent=MS.gene053875 |
| chr5.3 | exon | 43871217 | 43871324 | 43871217 | ID=MS.gene053875.t1.exon1;Parent=MS.gene053875.t1 |
| chr5.3 | CDS | 43871217 | 43871324 | 43871217 | ID=cds.MS.gene053875.t1;Parent=MS.gene053875.t1 |
| chr5.3 | exon | 43871709 | 43871861 | 43871709 | ID=MS.gene053875.t1.exon2;Parent=MS.gene053875.t1 |
| chr5.3 | CDS | 43871709 | 43871861 | 43871709 | ID=cds.MS.gene053875.t1;Parent=MS.gene053875.t1 |
| Gene Sequence |
| Protein sequence |
