AlfalfaGEDB | MS.gene81193

M. sativa cultivar XinJiangDaYe / MS.gene81193

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF02362.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81193.t1	XP_024640148.1	59	100	41	0	8	107	59	158	1.60E-27	132.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81193.t1	G7K4G4	59.0	100	41	0	8	107	709	808	1.2e-27	132.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049853	MS.gene81193	0.817843	2.67E-52	-1.69E-46
MS.gene051932	MS.gene81193	0.86303	3.33E-64	-1.69E-46
MS.gene052447	MS.gene81193	0.806603	7.59E-50	-1.69E-46
MS.gene053491	MS.gene81193	0.817055	4.02E-52	-1.69E-46
MS.gene053875	MS.gene81193	0.803236	3.84E-49	-1.69E-46
MS.gene054174	MS.gene81193	0.844856	5.83E-59	-1.69E-46
MS.gene054175	MS.gene81193	0.851789	7.06E-61	-1.69E-46
MS.gene05435	MS.gene81193	0.825025	5.87E-54	-1.69E-46
MS.gene054484	MS.gene81193	0.812421	4.28E-51	-1.69E-46
MS.gene058515	MS.gene81193	0.810209	1.29E-50	-1.69E-46
MS.gene059458	MS.gene81193	0.826851	2.16E-54	-1.69E-46
MS.gene059459	MS.gene81193	0.810464	1.14E-50	-1.69E-46
MS.gene059460	MS.gene81193	0.80828	3.35E-50	-1.69E-46
MS.gene06044	MS.gene81193	0.840306	9.41E-58	-1.69E-46
MS.gene061245	MS.gene81193	0.838031	3.66E-57	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81193.t1	MTR_5g094740	59.000	100	41	0	8	107	709	808	2.26e-36	130
MS.gene81193.t1	MTR_1g019580	55.102	98	44	0	8	105	19	116	9.64e-36	119
MS.gene81193.t1	MTR_1g105780	54.000	100	46	0	8	107	162	261	2.55e-35	122
MS.gene81193.t1	MTR_1g105765	54.000	100	46	0	8	107	162	261	2.69e-35	122
MS.gene81193.t1	MTR_4g062370	61.290	93	36	0	3	95	160	252	2.97e-32	119
MS.gene81193.t1	MTR_3g452180	49.038	104	53	0	2	105	71	174	1.90e-31	110
MS.gene81193.t1	MTR_4g032560	49.515	103	52	0	3	105	185	287	2.22e-31	112
MS.gene81193.t1	MTR_4g032580	49.515	103	52	0	3	105	185	287	2.47e-31	112
MS.gene81193.t1	MTR_7g110090	49.038	104	53	0	2	105	155	258	3.65e-31	111
MS.gene81193.t1	MTR_1g069260	46.281	121	50	2	8	113	166	286	7.41e-28	103
MS.gene81193.t1	MTR_4g121040	47.573	103	54	0	2	104	59	161	7.41e-27	98.2
MS.gene81193.t1	MTR_1g087870	43.158	95	54	0	9	103	115	209	2.47e-25	95.9
MS.gene81193.t1	MTR_1g047540	45.192	104	57	0	2	105	140	243	1.95e-24	96.3
MS.gene81193.t1	MTR_8g042540	54.795	73	33	0	35	107	3	75	3.01e-24	88.6
MS.gene81193.t1	MTR_8g042540	56.522	69	30	0	37	105	17	85	1.67e-23	87.0
MS.gene81193.t1	MTR_0152s0060	48.810	84	43	0	24	107	228	311	2.40e-22	90.1
MS.gene81193.t1	MTR_1g084880	41.860	86	50	0	20	105	178	263	8.94e-19	79.0
MS.gene81193.t1	MTR_3g048970	41.860	86	50	0	20	105	178	263	1.02e-18	79.0
MS.gene81193.t1	MTR_3g070250	38.835	103	60	1	13	112	176	278	7.45e-18	76.6
MS.gene81193.t1	MTR_2g016390	38.710	93	57	0	11	103	602	694	5.08e-17	75.5
MS.gene81193.t1	MTR_4g057880	47.059	68	33	1	48	112	2	69	6.07e-16	67.0
MS.gene81193.t1	MTR_4g081330	50.000	62	31	0	38	99	1085	1146	6.27e-15	69.7
MS.gene81193.t1	MTR_3g085990	41.176	102	41	3	4	105	140	222	8.12e-15	67.8
MS.gene81193.t1	MTR_2g100360	37.234	94	58	1	4	97	163	255	2.34e-14	67.0
MS.gene81193.t1	MTR_6g016445	50.000	60	30	0	42	101	13	72	1.76e-13	61.6
MS.gene81193.t1	MTR_1g037600	39.326	89	53	1	13	100	71	159	7.19e-13	63.2
MS.gene81193.t1	MTR_4g117740	49.123	57	29	0	6	62	141	197	1.59e-12	61.2
MS.gene81193.t1	MTR_2g072800	57.447	47	20	0	59	105	222	268	3.57e-12	61.2
MS.gene81193.t1	MTR_1g023840	37.209	86	53	1	16	100	11	96	9.76e-12	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 31 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GACTTTGTTGAGATCGATTA+TGG	0.218247	6.1:-55800912	MS.gene81193:CDS
TTGATGAATGTGGTTGTATA+TGG	0.268575	6.1:-55800733	MS.gene81193:CDS
AGATCCTTGCTATCTGTCTT+AGG	0.281334	6.1:-55800497	None:intergenic
ATGCAGGATTTGGCTCATAT+TGG	0.290632	6.1:-55800803	MS.gene81193:intron
TGAGATCGATTATGGATTCT+TGG	0.320901	6.1:-55800904	MS.gene81193:intron
TGCAGGATTTGGCTCATATT+GGG	0.361232	6.1:-55800802	MS.gene81193:intron
GGTCAAGGCTAGAAGGTTAA+AGG	0.364042	6.1:-55800642	MS.gene81193:CDS
TTCTCATTGACACCGATATA+TGG	0.377396	6.1:+55800593	None:intergenic
GACGGTGACAGTGTCGTTAT+CGG	0.398058	6.1:-55800620	MS.gene81193:CDS
TGCACAAATGGCACAGGAAA+AGG	0.399613	6.1:+55800975	None:intergenic
GTTATCGGTGCTCCATATAT+CGG	0.401878	6.1:-55800605	MS.gene81193:intron
CCTCATGCTCACTTTAAGAT+TGG	0.409855	6.1:-55800683	MS.gene81193:CDS
AGATTGGCGGTGGCTGGAAC+AGG	0.416190	6.1:-55800667	MS.gene81193:CDS
ACTTTAAGATTGGCGGTGGC+TGG	0.418939	6.1:-55800673	MS.gene81193:CDS
TGAAACTGAGAATCTGAGTT+TGG	0.418987	6.1:-55800756	MS.gene81193:CDS
TTAAATTCATTATGCACAAA+TGG	0.431684	6.1:+55800963	None:intergenic
CATGAGGGCGTGTAACAAAC+TGG	0.441451	6.1:+55800699	None:intergenic
ACAGGATGGTCAAGGCTAGA+AGG	0.443921	6.1:-55800649	MS.gene81193:CDS
CATGCTCACTTTAAGATTGG+CGG	0.482279	6.1:-55800680	MS.gene81193:CDS
CTGAGTTTGGTTGATGAATG+TGG	0.483514	6.1:-55800743	MS.gene81193:CDS
TGATGAATGTGGTTGTATAT+GGG	0.484155	6.1:-55800732	MS.gene81193:CDS
GCTCACTTTAAGATTGGCGG+TGG	0.568885	6.1:-55800677	MS.gene81193:CDS
CCAATCTTAAAGTGAGCATG+AGG	0.578221	6.1:+55800683	None:intergenic
TCATTATGCACAAATGGCAC+AGG	0.601819	6.1:+55800969	None:intergenic
AAATCCTAAGACAGATAGCA+AGG	0.605831	6.1:+55800493	None:intergenic
TGGCGGTGGCTGGAACAGGA+TGG	0.649155	6.1:-55800663	MS.gene81193:CDS
TGGCTGGAACAGGATGGTCA+AGG	0.676417	6.1:-55800657	MS.gene81193:CDS
AGGGCGTGTAACAAACTGGA+GGG	0.676724	6.1:+55800703	None:intergenic
CAATCTTAAAGTGAGCATGA+GGG	0.680650	6.1:+55800684	None:intergenic
AAGGCTAGAAGGTTAAAGGA+CGG	0.693112	6.1:-55800638	MS.gene81193:CDS
GAGGGCGTGTAACAAACTGG+AGG	0.699235	6.1:+55800702	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTAAATTCATTATGCACAAA+TGG	+	chr6.1:55800528-55800547	None:intergenic	20.0%
!!!	TGTTAAAAAAGATATTGACT+TGG	-	chr6.1:55800618-55800637	MS.gene81193:CDS	20.0%
!!!	ATATGATGTTTTGATTATGC+AGG	-	chr6.1:55800669-55800688	MS.gene81193:CDS	25.0%
!!!	TTTTGTTGTTGTTCGTTATT+AGG	-	chr6.1:55800926-55800945	MS.gene81193:CDS	25.0%
	TGATGAATGTGGTTGTATAT+GGG	-	chr6.1:55800756-55800775	MS.gene81193:CDS	30.0%
	TTGATGAATGTGGTTGTATA+TGG	-	chr6.1:55800755-55800774	MS.gene81193:CDS	30.0%
!!!	TGTTTTGATTATGCAGGATT+TGG	-	chr6.1:55800675-55800694	MS.gene81193:CDS	30.0%
	CAATCTTAAAGTGAGCATGA+GGG	+	chr6.1:55800807-55800826	None:intergenic	35.0%
	GACTTTGTTGAGATCGATTA+TGG	-	chr6.1:55800576-55800595	MS.gene81193:intron	35.0%
	TGAAACTGAGAATCTGAGTT+TGG	-	chr6.1:55800732-55800751	MS.gene81193:CDS	35.0%
	TGAGATCGATTATGGATTCT+TGG	-	chr6.1:55800584-55800603	MS.gene81193:intron	35.0%
	TTCTCATTGACACCGATATA+TGG	+	chr6.1:55800898-55800917	None:intergenic	35.0%
	AAGGCTAGAAGGTTAAAGGA+CGG	-	chr6.1:55800850-55800869	MS.gene81193:intron	40.0%
	CCAATCTTAAAGTGAGCATG+AGG	+	chr6.1:55800808-55800827	None:intergenic	40.0%
	TCATTATGCACAAATGGCAC+AGG	+	chr6.1:55800522-55800541	None:intergenic	40.0%
!	ATGCAGGATTTGGCTCATAT+TGG	-	chr6.1:55800685-55800704	MS.gene81193:CDS	40.0%
!	GTTATCGGTGCTCCATATAT+CGG	-	chr6.1:55800883-55800902	MS.gene81193:intron	40.0%
!	TGCAGGATTTGGCTCATATT+GGG	-	chr6.1:55800686-55800705	MS.gene81193:CDS	40.0%
!!	CATGCTCACTTTAAGATTGG+CGG	-	chr6.1:55800808-55800827	MS.gene81193:intron	40.0%
!!	CCTCATGCTCACTTTAAGAT+TGG	-	chr6.1:55800805-55800824	MS.gene81193:intron	40.0%
!!	CTGAGTTTGGTTGATGAATG+TGG	-	chr6.1:55800745-55800764	MS.gene81193:CDS	40.0%
	GGTCAAGGCTAGAAGGTTAA+AGG	-	chr6.1:55800846-55800865	MS.gene81193:intron	45.0%
	TGCACAAATGGCACAGGAAA+AGG	+	chr6.1:55800516-55800535	None:intergenic	45.0%
	ACAGGATGGTCAAGGCTAGA+AGG	-	chr6.1:55800839-55800858	MS.gene81193:intron	50.0%
	AGGGCGTGTAACAAACTGGA+GGG	+	chr6.1:55800788-55800807	None:intergenic	50.0%
!	CATGAGGGCGTGTAACAAAC+TGG	+	chr6.1:55800792-55800811	None:intergenic	50.0%
!	GACGGTGACAGTGTCGTTAT+CGG	-	chr6.1:55800868-55800887	MS.gene81193:intron	50.0%
!!	ACTTTAAGATTGGCGGTGGC+TGG	-	chr6.1:55800815-55800834	MS.gene81193:intron	50.0%
!!	GCTCACTTTAAGATTGGCGG+TGG	-	chr6.1:55800811-55800830	MS.gene81193:intron	50.0%
	TGGCTGGAACAGGATGGTCA+AGG	-	chr6.1:55800831-55800850	MS.gene81193:intron	55.0%
!	GAGGGCGTGTAACAAACTGG+AGG	+	chr6.1:55800789-55800808	None:intergenic	55.0%
	AGATTGGCGGTGGCTGGAAC+AGG	-	chr6.1:55800821-55800840	MS.gene81193:intron	60.0%
	TGGCGGTGGCTGGAACAGGA+TGG	-	chr6.1:55800825-55800844	MS.gene81193:intron	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.1	gene	55800498	55801012	55800498	ID=MS.gene81193
chr6.1	mRNA	55800498	55801012	55800498	ID=MS.gene81193.t1;Parent=MS.gene81193
chr6.1	exon	55800905	55801012	55800905	ID=MS.gene81193.t1.exon1;Parent=MS.gene81193.t1
chr6.1	CDS	55800905	55801012	55800905	ID=cds.MS.gene81193.t1;Parent=MS.gene81193.t1
chr6.1	exon	55800606	55800819	55800606	ID=MS.gene81193.t1.exon2;Parent=MS.gene81193.t1
chr6.1	CDS	55800606	55800819	55800606	ID=cds.MS.gene81193.t1;Parent=MS.gene81193.t1
chr6.1	exon	55800498	55800517	55800498	ID=MS.gene81193.t1.exon3;Parent=MS.gene81193.t1
chr6.1	CDS	55800498	55800517	55800498	ID=cds.MS.gene81193.t1;Parent=MS.gene81193.t1

Gene Sequence

>MS.gene81193

ATGCATTTGTTCAATCCTTTTCCTGTGCCATTTGTGCATAATGAATTTAACTTTCAACTGACATATGAGAAAAAGCTTGACTTTGTTGAGATCGATTATGGATTCTTGGTATGTTTTATGTGTTAAAAAAGATATTGACTTGGTGTTGCAATTGATTCGACTTCAACTAATATATGATGTTTTGATTATGCAGGATTTGGCTCATATTGGGTTCTGTCAGCTTGCTTTGAACACTGAAACTGAGAATCTGAGTTTGGTTGATGAATGTGGTTGTATATGGGATTGCACCCTCCAGTTTGTTACACGCCCTCATGCTCACTTTAAGATTGGCGGTGGCTGGAACAGGATGGTCAAGGCTAGAAGGTTAAAGGACGGTGACAGTGTCGTTATCGGTGCTCCATATATCGGTGTCAATGAGAAGCTCTTTTTTTTGTTGTTGTTCGTTATTAGGAAGTTGCTATCTATTTACGTTTTCTGTCATTTGTTGTTTCACAGATCCTTGCTATCTGTCTTAG

Protein sequence

>MS.gene81193.t1

MHLFNPFPVPFVHNEFNFQLTYEKKLDFVEIDYGFLDLAHIGFCQLALNTETENLSLVDECGCIWDCTLQFVTRPHAHFKIGGGWNRMVKARRLKDGDSVVIGAPYIDPCYLS