Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24211.t1 | PNX61034.1 | 72.9 | 96 | 22 | 1 | 1 | 96 | 1 | 92 | 6.10E-31 | 143.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24211.t1 | A0A2K3K421 | 72.9 | 96 | 22 | 1 | 1 | 96 | 1 | 92 | 4.4e-31 | 143.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049262 | MS.gene24211 | 0.824351 | 8.46E-54 | -1.69E-46 |
| MS.gene049853 | MS.gene24211 | 0.839754 | 1.31E-57 | -1.69E-46 |
| MS.gene049968 | MS.gene24211 | 0.803021 | 4.25E-49 | -1.69E-46 |
| MS.gene050611 | MS.gene24211 | 0.801751 | 7.77E-49 | -1.69E-46 |
| MS.gene052447 | MS.gene24211 | 0.815035 | 1.14E-51 | -1.69E-46 |
| MS.gene05329 | MS.gene24211 | 0.802365 | 5.81E-49 | -1.69E-46 |
| MS.gene053512 | MS.gene24211 | 0.810602 | 1.06E-50 | -1.69E-46 |
| MS.gene053875 | MS.gene24211 | 0.800474 | 1.42E-48 | -1.69E-46 |
| MS.gene053974 | MS.gene24211 | 0.828332 | 9.54E-55 | -1.69E-46 |
| MS.gene054174 | MS.gene24211 | 0.821385 | 4.15E-53 | -1.69E-46 |
| MS.gene054175 | MS.gene24211 | 0.826992 | 2.00E-54 | -1.69E-46 |
| MS.gene05435 | MS.gene24211 | 0.82179 | 3.35E-53 | -1.69E-46 |
| MS.gene054484 | MS.gene24211 | 0.807584 | 4.71E-50 | -1.69E-46 |
| MS.gene055017 | MS.gene24211 | 0.82584 | 3.76E-54 | -1.69E-46 |
| MS.gene055056 | MS.gene24211 | 0.84053 | 8.23E-58 | -1.69E-46 |
| MS.gene055058 | MS.gene24211 | 0.808199 | 3.48E-50 | -1.69E-46 |
| MS.gene05531 | MS.gene24211 | 0.804511 | 2.09E-49 | -1.69E-46 |
| MS.gene05762 | MS.gene24211 | 0.82247 | 2.33E-53 | -1.69E-46 |
| MS.gene058515 | MS.gene24211 | 0.806091 | 9.73E-50 | -1.69E-46 |
| MS.gene058580 | MS.gene24211 | 0.829169 | 5.98E-55 | -1.69E-46 |
| MS.gene05925 | MS.gene24211 | 0.800028 | 1.75E-48 | -1.69E-46 |
| MS.gene059276 | MS.gene24211 | 0.830379 | 3.04E-55 | -1.69E-46 |
| MS.gene059458 | MS.gene24211 | 0.830752 | 2.46E-55 | -1.69E-46 |
| MS.gene059459 | MS.gene24211 | 0.816637 | 4.99E-52 | -1.69E-46 |
| MS.gene059460 | MS.gene24211 | 0.817519 | 3.16E-52 | -1.69E-46 |
| MS.gene059584 | MS.gene24211 | 0.808376 | 3.19E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24211.t1 | MTR_1g009820 | 57.143 | 98 | 38 | 1 | 1 | 98 | 1 | 94 | 2.82e-38 | 124 |
| MS.gene24211.t1 | MTR_8g089760 | 55.952 | 84 | 33 | 1 | 1 | 84 | 78 | 157 | 2.34e-27 | 98.6 |
| MS.gene24211.t1 | MTR_4g007830 | 55.952 | 84 | 33 | 1 | 1 | 84 | 261 | 340 | 3.42e-25 | 96.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAAGTTTCAAATCAACTTA+TGG | 0.227227 | 1.2:+1586555 | MS.gene24211:CDS |
| TAGGAATGATTGTGCGATTT+GGG | 0.304560 | 1.2:+1586747 | MS.gene24211:intron |
| TTTCATGGAAAACTTGTTGA+TGG | 0.314872 | 1.2:+1586509 | None:intergenic |
| ATCAAATGGATGATGGAAAA+AGG | 0.314997 | 1.2:+1586772 | MS.gene24211:CDS |
| TTAGGAATGATTGTGCGATT+TGG | 0.345924 | 1.2:+1586746 | MS.gene24211:intron |
| GGATTTGATCTTGCGGAATT+CGG | 0.384621 | 1.2:+1586971 | MS.gene24211:CDS |
| GTGCGATTTGGGTAATCAAA+TGG | 0.465432 | 1.2:+1586758 | MS.gene24211:CDS |
| ATGGATCCCTCTTTCTATGT+GGG | 0.495254 | 1.2:+1586528 | MS.gene24211:CDS |
| TTTGTTGCTCCACGTGGTTA+CGG | 0.500598 | 1.2:+1586600 | MS.gene24211:CDS |
| GATGGATCCCTCTTTCTATG+TGG | 0.506410 | 1.2:+1586527 | MS.gene24211:CDS |
| TTGGGTAATCAAATGGATGA+TGG | 0.515751 | 1.2:+1586765 | MS.gene24211:CDS |
| GATCCCTCTTTCTATGTGGG+TGG | 0.554391 | 1.2:+1586531 | MS.gene24211:CDS |
| TGAACCACCCACATAGAAAG+AGG | 0.556566 | 1.2:-1586535 | None:intergenic |
| TTACACTAACAGGTGGATGA+AGG | 0.560129 | 1.2:+1586894 | MS.gene24211:intron |
| CCACGTGGTTACGGAAGTCA+AGG | 0.569221 | 1.2:+1586609 | MS.gene24211:CDS |
| AAAAGAGGGATTTGATCTTG+CGG | 0.622365 | 1.2:+1586964 | MS.gene24211:CDS |
| TTTAAGTTTGTTGCTCCACG+TGG | 0.650344 | 1.2:+1586594 | MS.gene24211:CDS |
| GAACCACCCACATAGAAAGA+GGG | 0.662397 | 1.2:-1586534 | None:intergenic |
| CCTTGACTTCCGTAACCACG+TGG | 0.696038 | 1.2:-1586609 | None:intergenic |
| CACGTGGTTACGGAAGTCAA+GGG | 0.703622 | 1.2:+1586610 | MS.gene24211:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATACTCATAATTTTTTATTT+AGG | + | chr1.2:1586728-1586747 | MS.gene24211:intron | 10.0% |
| !!! | TTTTTTATATCTTACGAGTT+TGG | - | chr1.2:1586636-1586655 | None:intergenic | 20.0% |
| ! | TCAAGTTTCAAATCAACTTA+TGG | + | chr1.2:1586555-1586574 | MS.gene24211:CDS | 25.0% |
| !! | AAATCCCTCTTTTTATTGTA+AGG | - | chr1.2:1586957-1586976 | None:intergenic | 25.0% |
| GCATCCTTACAATAAAAAGA+GGG | + | chr1.2:1586950-1586969 | MS.gene24211:CDS | 30.0% | |
| TGCATCCTTACAATAAAAAG+AGG | + | chr1.2:1586949-1586968 | MS.gene24211:CDS | 30.0% | |
| TTTACCACAATTACACTAAC+AGG | + | chr1.2:1586884-1586903 | MS.gene24211:intron | 30.0% | |
| !! | ATCAAATGGATGATGGAAAA+AGG | + | chr1.2:1586772-1586791 | MS.gene24211:CDS | 30.0% |
| AAAAGAGGGATTTGATCTTG+CGG | + | chr1.2:1586964-1586983 | MS.gene24211:CDS | 35.0% | |
| TAGGAATGATTGTGCGATTT+GGG | + | chr1.2:1586747-1586766 | MS.gene24211:intron | 35.0% | |
| TTAGGAATGATTGTGCGATT+TGG | + | chr1.2:1586746-1586765 | MS.gene24211:intron | 35.0% | |
| TTGGGTAATCAAATGGATGA+TGG | + | chr1.2:1586765-1586784 | MS.gene24211:CDS | 35.0% | |
| ACCACAATTACACTAACAGG+TGG | + | chr1.2:1586887-1586906 | MS.gene24211:intron | 40.0% | |
| ATGGATCCCTCTTTCTATGT+GGG | + | chr1.2:1586528-1586547 | MS.gene24211:CDS | 40.0% | |
| GGATTTGATCTTGCGGAATT+CGG | + | chr1.2:1586971-1586990 | MS.gene24211:CDS | 40.0% | |
| TCCACCTGTTAGTGTAATTG+TGG | - | chr1.2:1586891-1586910 | None:intergenic | 40.0% | |
| TTACACTAACAGGTGGATGA+AGG | + | chr1.2:1586894-1586913 | MS.gene24211:intron | 40.0% | |
| ! | GTGCGATTTGGGTAATCAAA+TGG | + | chr1.2:1586758-1586777 | MS.gene24211:CDS | 40.0% |
| !! | TTTAAGTTTGTTGCTCCACG+TGG | + | chr1.2:1586594-1586613 | MS.gene24211:CDS | 40.0% |
| GAACCACCCACATAGAAAGA+GGG | - | chr1.2:1586537-1586556 | None:intergenic | 45.0% | |
| GATGGATCCCTCTTTCTATG+TGG | + | chr1.2:1586527-1586546 | MS.gene24211:CDS | 45.0% | |
| TGAACCACCCACATAGAAAG+AGG | - | chr1.2:1586538-1586557 | None:intergenic | 45.0% | |
| TTTGTTGCTCCACGTGGTTA+CGG | + | chr1.2:1586600-1586619 | MS.gene24211:CDS | 45.0% | |
| CACGTGGTTACGGAAGTCAA+GGG | + | chr1.2:1586610-1586629 | MS.gene24211:CDS | 50.0% | |
| GATCCCTCTTTCTATGTGGG+TGG | + | chr1.2:1586531-1586550 | MS.gene24211:CDS | 50.0% | |
| CCACGTGGTTACGGAAGTCA+AGG | + | chr1.2:1586609-1586628 | MS.gene24211:CDS | 55.0% | |
| CCTTGACTTCCGTAACCACG+TGG | - | chr1.2:1586612-1586631 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 1586513 | 1587007 | 1586513 | ID=MS.gene24211 |
| chr1.2 | mRNA | 1586513 | 1587007 | 1586513 | ID=MS.gene24211.t1;Parent=MS.gene24211 |
| chr1.2 | exon | 1586513 | 1586640 | 1586513 | ID=MS.gene24211.t1.exon1;Parent=MS.gene24211.t1 |
| chr1.2 | CDS | 1586513 | 1586640 | 1586513 | ID=cds.MS.gene24211.t1;Parent=MS.gene24211.t1 |
| chr1.2 | exon | 1586750 | 1586816 | 1586750 | ID=MS.gene24211.t1.exon2;Parent=MS.gene24211.t1 |
| chr1.2 | CDS | 1586750 | 1586816 | 1586750 | ID=cds.MS.gene24211.t1;Parent=MS.gene24211.t1 |
| chr1.2 | exon | 1586906 | 1587007 | 1586906 | ID=MS.gene24211.t1.exon3;Parent=MS.gene24211.t1 |
| chr1.2 | CDS | 1586906 | 1587007 | 1586906 | ID=cds.MS.gene24211.t1;Parent=MS.gene24211.t1 |
| Gene Sequence |
| Protein sequence |
