Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79824.t1 | AET04582.1 | 74 | 50 | 13 | 0 | 2 | 51 | 164 | 213 | 6.60E-10 | 73.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79824.t1 | G7LBA2 | 74.0 | 50 | 13 | 0 | 2 | 51 | 164 | 213 | 4.8e-10 | 73.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049262 | MS.gene79824 | 0.805126 | 1.55E-49 | -1.69E-46 |
| MS.gene049853 | MS.gene79824 | 0.863589 | 2.23E-64 | -1.69E-46 |
| MS.gene052447 | MS.gene79824 | 0.836692 | 8.06E-57 | -1.69E-46 |
| MS.gene05329 | MS.gene79824 | 0.805148 | 1.54E-49 | -1.69E-46 |
| MS.gene053875 | MS.gene79824 | 0.803799 | 2.93E-49 | -1.69E-46 |
| MS.gene05404 | MS.gene79824 | 0.817922 | 2.56E-52 | -1.69E-46 |
| MS.gene054174 | MS.gene79824 | 0.873274 | 1.67E-67 | -1.69E-46 |
| MS.gene054175 | MS.gene79824 | 0.878315 | 3.10E-69 | -1.69E-46 |
| MS.gene054484 | MS.gene79824 | 0.845568 | 3.74E-59 | -1.69E-46 |
| MS.gene055017 | MS.gene79824 | 0.81024 | 1.27E-50 | -1.69E-46 |
| MS.gene055058 | MS.gene79824 | 0.822325 | 2.52E-53 | -1.69E-46 |
| MS.gene055952 | MS.gene79824 | 0.809545 | 1.79E-50 | -1.69E-46 |
| MS.gene05762 | MS.gene79824 | 0.806828 | 6.80E-50 | -1.69E-46 |
| MS.gene058515 | MS.gene79824 | 0.816111 | 6.54E-52 | -1.69E-46 |
| MS.gene058580 | MS.gene79824 | 0.809549 | 1.79E-50 | -1.69E-46 |
| MS.gene059276 | MS.gene79824 | 0.813574 | 2.39E-51 | -1.69E-46 |
| MS.gene059458 | MS.gene79824 | 0.811618 | 6.40E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79824.t1 | MTR_8g091850 | 64.062 | 64 | 20 | 1 | 2 | 65 | 164 | 224 | 1.36e-17 | 74.7 |
| MS.gene79824.t1 | MTR_8g091830 | 60.870 | 46 | 18 | 0 | 2 | 47 | 495 | 540 | 6.29e-11 | 57.0 |
| MS.gene79824.t1 | MTR_8g091830 | 60.870 | 46 | 18 | 0 | 2 | 47 | 497 | 542 | 6.77e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACATAGTGTACCTTTGATT+GGG | 0.371173 | 6.3:-50885906 | None:intergenic |
| TAACATAGTGTACCTTTGAT+TGG | 0.378122 | 6.3:-50885907 | None:intergenic |
| GCATGATAGATGTGACAGTA+TGG | 0.460512 | 6.3:+50886334 | MS.gene79824:CDS |
| GGTGTAAGCTATTTATTCGA+AGG | 0.521459 | 6.3:-50886613 | None:intergenic |
| TGCAGAATGCATGGAGCGAT+CGG | 0.529205 | 6.3:+50885746 | MS.gene79824:CDS |
| AAATTGGTTCAGGCGGATTG+AGG | 0.538560 | 6.3:+50886305 | MS.gene79824:intron |
| TATTCAATGTGCAGAATGCA+TGG | 0.546050 | 6.3:+50885737 | MS.gene79824:CDS |
| ACATAGTGTACCTTTGATTG+GGG | 0.574989 | 6.3:-50885905 | None:intergenic |
| GGTTCAGGCGGATTGAGGCA+TGG | 0.576094 | 6.3:+50886310 | MS.gene79824:intron |
| CATTGAATAATATTTCGAAG+AGG | 0.659476 | 6.3:-50885723 | None:intergenic |
| AGAATGCATGGAGCGATCGG+CGG | 0.668966 | 6.3:+50885749 | MS.gene79824:CDS |
| TAGATTCTCATCAACACTCG+CGG | 0.677378 | 6.3:-50886581 | None:intergenic |
| CATAGTGTACCTTTGATTGG+GGG | 0.683143 | 6.3:-50885904 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATACTTTCAGATTAAAAA+AGG | - | chr6.3:50886001-50886020 | None:intergenic | 15.0% |
| !! | TTTCTACATAAAGAAACAAA+TGG | - | chr6.3:50885856-50885875 | None:intergenic | 20.0% |
| !!! | TTGTTTCTTTATGTAGAAAA+AGG | + | chr6.3:50885857-50885876 | MS.gene79824:intron | 20.0% |
| ! | ACATAAAGAAACAAATGGAA+CGG | - | chr6.3:50885851-50885870 | None:intergenic | 25.0% |
| ! | ATTATGACTTCTTATATGCA+GGG | + | chr6.3:50886550-50886569 | MS.gene79824:intron | 25.0% |
| ! | CATTGAATAATATTTCGAAG+AGG | - | chr6.3:50885726-50885745 | None:intergenic | 25.0% |
| ! | GTAACTTAAAACTAACTGAA+GGG | - | chr6.3:50885936-50885955 | None:intergenic | 25.0% |
| ! | TGTAACTTAAAACTAACTGA+AGG | - | chr6.3:50885937-50885956 | None:intergenic | 25.0% |
| ! | TTTCTTTATGTAGAAAAAGG+AGG | + | chr6.3:50885860-50885879 | MS.gene79824:intron | 25.0% |
| !! | AATCAGGCTATAATTTTATC+AGG | - | chr6.3:50886533-50886552 | None:intergenic | 25.0% |
| !! | ATTTTCATCCTTGAGAAAAT+TGG | + | chr6.3:50886289-50886308 | MS.gene79824:intron | 25.0% |
| !! | TATAATTTTATCAGGCTATC+AGG | - | chr6.3:50886525-50886544 | None:intergenic | 25.0% |
| AAATCACTGAATGAAAGATG+TGG | - | chr6.3:50886201-50886220 | None:intergenic | 30.0% | |
| AACATAGTGTACCTTTGATT+GGG | - | chr6.3:50885909-50885928 | None:intergenic | 30.0% | |
| ATAATAGGACAGCAACAAAA+TGG | - | chr6.3:50886500-50886519 | None:intergenic | 30.0% | |
| CATTGACCAGAAAATAAATG+TGG | - | chr6.3:50885974-50885993 | None:intergenic | 30.0% | |
| GATTATGACTTCTTATATGC+AGG | + | chr6.3:50886549-50886568 | MS.gene79824:intron | 30.0% | |
| GCATATAAGAAGTCATAATC+AGG | - | chr6.3:50886549-50886568 | None:intergenic | 30.0% | |
| TAACATAGTGTACCTTTGAT+TGG | - | chr6.3:50885910-50885929 | None:intergenic | 30.0% | |
| TGTACAATAGTTCCTAACTT+GGG | - | chr6.3:50886405-50886424 | None:intergenic | 30.0% | |
| ! | TTGTTCAAAATTTTGCACGA+TGG | + | chr6.3:50886240-50886259 | MS.gene79824:intron | 30.0% |
| !! | CAAATGCCACATTTATTTTC+TGG | + | chr6.3:50885965-50885984 | MS.gene79824:intron | 30.0% |
| AAAGAAACAAATGGAACGGT+TGG | - | chr6.3:50885847-50885866 | None:intergenic | 35.0% | |
| AACTTGGGACTAAATAGAAG+AGG | - | chr6.3:50886390-50886409 | None:intergenic | 35.0% | |
| AAGATGTGGTGTATGTCATA+TGG | - | chr6.3:50886187-50886206 | None:intergenic | 35.0% | |
| ACATAGTGTACCTTTGATTG+GGG | - | chr6.3:50885908-50885927 | None:intergenic | 35.0% | |
| CATTGCTAGCATTTGACATT+AGG | + | chr6.3:50885802-50885821 | MS.gene79824:intron | 35.0% | |
| CTGTACAATAGTTCCTAACT+TGG | - | chr6.3:50886406-50886425 | None:intergenic | 35.0% | |
| CTTCTATTTAGTCCCAAGTT+AGG | + | chr6.3:50886390-50886409 | MS.gene79824:intron | 35.0% | |
| TATTCAATGTGCAGAATGCA+TGG | + | chr6.3:50885737-50885756 | MS.gene79824:CDS | 35.0% | |
| TTCAATAGCAAGAGACTGTA+AGG | - | chr6.3:50886086-50886105 | None:intergenic | 35.0% | |
| ! | ATCCTTGAGAAAATTGGTTC+AGG | + | chr6.3:50886295-50886314 | MS.gene79824:intron | 35.0% |
| AAACATCATCCCCCAATCAA+AGG | + | chr6.3:50885895-50885914 | MS.gene79824:CDS | 40.0% | |
| ATGTGGTGTATGTCATATGG+TGG | - | chr6.3:50886184-50886203 | None:intergenic | 40.0% | |
| CATAGTGTACCTTTGATTGG+GGG | - | chr6.3:50885907-50885926 | None:intergenic | 40.0% | |
| GACTTCTTATATGCAGGGAT+TGG | + | chr6.3:50886555-50886574 | MS.gene79824:intron | 40.0% | |
| GCATGATAGATGTGACAGTA+TGG | + | chr6.3:50886334-50886353 | MS.gene79824:CDS | 40.0% | |
| TAGATTCTCATCAACACTCG+CGG | - | chr6.3:50886584-50886603 | None:intergenic | 40.0% | |
| TAGTGCACTTCTTGACAACA+TGG | - | chr6.3:50886270-50886289 | None:intergenic | 40.0% | |
| TCAGGCTATCAGGCTATAAT+AGG | - | chr6.3:50886515-50886534 | None:intergenic | 40.0% | |
| ! | CTTGAGAAAATTGGTTCAGG+CGG | + | chr6.3:50886298-50886317 | MS.gene79824:intron | 40.0% |
| ! | CGCCTGAACCAATTTTCTCA+AGG | - | chr6.3:50886300-50886319 | None:intergenic | 45.0% |
| !! | AAATTGGTTCAGGCGGATTG+AGG | + | chr6.3:50886305-50886324 | MS.gene79824:intron | 45.0% |
| TGCAGAATGCATGGAGCGAT+CGG | + | chr6.3:50885746-50885765 | MS.gene79824:CDS | 50.0% | |
| AGAATGCATGGAGCGATCGG+CGG | + | chr6.3:50885749-50885768 | MS.gene79824:CDS | 55.0% | |
| !! | GGTTCAGGCGGATTGAGGCA+TGG | + | chr6.3:50886310-50886329 | MS.gene79824:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 50885672 | 50886627 | 50885672 | ID=MS.gene79824 |
| chr6.3 | mRNA | 50885672 | 50886627 | 50885672 | ID=MS.gene79824.t1;Parent=MS.gene79824 |
| chr6.3 | exon | 50885672 | 50885770 | 50885672 | ID=MS.gene79824.t1.exon1;Parent=MS.gene79824.t1 |
| chr6.3 | CDS | 50885672 | 50885770 | 50885672 | ID=cds.MS.gene79824.t1;Parent=MS.gene79824.t1 |
| chr6.3 | exon | 50885873 | 50885916 | 50885873 | ID=MS.gene79824.t1.exon2;Parent=MS.gene79824.t1 |
| chr6.3 | CDS | 50885873 | 50885916 | 50885873 | ID=cds.MS.gene79824.t1;Parent=MS.gene79824.t1 |
| chr6.3 | exon | 50886317 | 50886371 | 50886317 | ID=MS.gene79824.t1.exon3;Parent=MS.gene79824.t1 |
| chr6.3 | CDS | 50886317 | 50886371 | 50886317 | ID=cds.MS.gene79824.t1;Parent=MS.gene79824.t1 |
| chr6.3 | exon | 50886571 | 50886627 | 50886571 | ID=MS.gene79824.t1.exon4;Parent=MS.gene79824.t1 |
| chr6.3 | CDS | 50886571 | 50886627 | 50886571 | ID=cds.MS.gene79824.t1;Parent=MS.gene79824.t1 |
| Gene Sequence |
| Protein sequence |
