Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62748.t1 | XP_024633451.1 | 84.8 | 46 | 5 | 1 | 1 | 44 | 26 | 71 | 4.60E-09 | 70.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62748.t1 | G7L456 | 48.8 | 84 | 29 | 2 | 1 | 70 | 1 | 84 | 9.9e-06 | 59.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049262 | MS.gene62748 | 0.821606 | 3.69E-53 | -1.69E-46 |
| MS.gene049587 | MS.gene62748 | 0.825078 | 5.70E-54 | -1.69E-46 |
| MS.gene049853 | MS.gene62748 | 0.868719 | 5.30E-66 | -1.69E-46 |
| MS.gene049968 | MS.gene62748 | 0.811178 | 7.97E-51 | -1.69E-46 |
| MS.gene050430 | MS.gene62748 | 0.814828 | 1.26E-51 | -1.69E-46 |
| MS.gene050611 | MS.gene62748 | 0.802197 | 6.29E-49 | -1.69E-46 |
| MS.gene051932 | MS.gene62748 | 0.812052 | 5.15E-51 | -1.69E-46 |
| MS.gene052447 | MS.gene62748 | 0.811282 | 7.57E-51 | -1.69E-46 |
| MS.gene053512 | MS.gene62748 | 0.808739 | 2.67E-50 | -1.69E-46 |
| MS.gene053875 | MS.gene62748 | 0.803738 | 3.02E-49 | -1.69E-46 |
| MS.gene053974 | MS.gene62748 | 0.804055 | 2.60E-49 | -1.69E-46 |
| MS.gene054174 | MS.gene62748 | 0.852765 | 3.72E-61 | -1.69E-46 |
| MS.gene054175 | MS.gene62748 | 0.858015 | 1.10E-62 | -1.69E-46 |
| MS.gene05435 | MS.gene62748 | 0.80214 | 6.46E-49 | -1.69E-46 |
| MS.gene054484 | MS.gene62748 | 0.855251 | 7.15E-62 | -1.69E-46 |
| MS.gene055017 | MS.gene62748 | 0.830132 | 3.49E-55 | -1.69E-46 |
| MS.gene055056 | MS.gene62748 | 0.826264 | 2.99E-54 | -1.69E-46 |
| MS.gene055058 | MS.gene62748 | 0.833588 | 4.89E-56 | -1.69E-46 |
| MS.gene055952 | MS.gene62748 | 0.826887 | 2.12E-54 | -1.69E-46 |
| MS.gene05762 | MS.gene62748 | 0.834155 | 3.53E-56 | -1.69E-46 |
| MS.gene058515 | MS.gene62748 | 0.832784 | 7.76E-56 | -1.69E-46 |
| MS.gene058580 | MS.gene62748 | 0.808196 | 3.49E-50 | -1.69E-46 |
| MS.gene059276 | MS.gene62748 | 0.85138 | 9.21E-61 | -1.69E-46 |
| MS.gene059458 | MS.gene62748 | 0.815111 | 1.09E-51 | -1.69E-46 |
| MS.gene059459 | MS.gene62748 | 0.80523 | 1.48E-49 | -1.69E-46 |
| MS.gene059584 | MS.gene62748 | 0.810795 | 9.65E-51 | -1.69E-46 |
| MS.gene06044 | MS.gene62748 | 0.802131 | 6.49E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62748.t1 | MTR_7g012300 | 57.500 | 120 | 36 | 4 | 1 | 105 | 1 | 120 | 2.13e-27 | 98.6 |
| MS.gene62748.t1 | MTR_2g043940 | 72.414 | 58 | 16 | 0 | 59 | 116 | 305 | 362 | 9.51e-17 | 74.7 |
| MS.gene62748.t1 | MTR_7g056577 | 66.667 | 66 | 17 | 1 | 56 | 116 | 47 | 112 | 3.37e-16 | 69.7 |
| MS.gene62748.t1 | MTR_5g061300 | 54.545 | 99 | 37 | 3 | 21 | 112 | 229 | 326 | 8.79e-16 | 71.6 |
| MS.gene62748.t1 | MTR_5g061660 | 60.000 | 75 | 16 | 1 | 56 | 116 | 71 | 145 | 1.09e-13 | 63.9 |
| MS.gene62748.t1 | MTR_0039s0180 | 63.934 | 61 | 18 | 1 | 57 | 113 | 58 | 118 | 1.79e-13 | 62.4 |
| MS.gene62748.t1 | MTR_2g461540 | 60.000 | 75 | 16 | 2 | 56 | 116 | 3 | 77 | 1.10e-12 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAACCATTTCTTGAAGTT+GGG | 0.352117 | 7.3:+65343603 | None:intergenic |
| TTTCAACCATTTCTTGAAGT+TGG | 0.356872 | 7.3:+65343602 | None:intergenic |
| CTAGTCCCAACTTCAAGAAA+TGG | 0.372385 | 7.3:-65343608 | MS.gene62748:intron |
| AAGCAATTGGATGTAGAAAC+TGG | 0.389575 | 7.3:-65343736 | MS.gene62748:CDS |
| ATGGGAAAGGAGTGTGTACC+AGG | 0.471236 | 7.3:-65343544 | MS.gene62748:intron |
| CTTGAGGACAACATAGGTGA+TGG | 0.475620 | 7.3:-65343095 | MS.gene62748:CDS |
| ACTTCAAGAAATGGTTGAAA+AGG | 0.483448 | 7.3:-65343599 | MS.gene62748:CDS |
| AGGAAGTAGAGGAAAGCAAT+TGG | 0.498254 | 7.3:-65343749 | MS.gene62748:CDS |
| ATGAAATTCAAGAGGATGAT+CGG | 0.537624 | 7.3:-65343022 | MS.gene62748:CDS |
| GAATTTCTTGAGGACAACAT+AGG | 0.539954 | 7.3:-65343101 | MS.gene62748:CDS |
| TCAAGAAATGGTTGAAAAGG+AGG | 0.541538 | 7.3:-65343596 | MS.gene62748:CDS |
| TTGAGGACAACATAGGTGAT+GGG | 0.545907 | 7.3:-65343094 | MS.gene62748:CDS |
| CGCATATCATGAATTTCTTG+AGG | 0.553600 | 7.3:-65343111 | MS.gene62748:CDS |
| GAAACTCGACAAGGAAGTAG+AGG | 0.581711 | 7.3:-65343760 | MS.gene62748:CDS |
| TATATATGATGAATTTCAAG+AGG | 0.613332 | 7.3:-65343057 | MS.gene62748:CDS |
| TCTAGATGATGAAATTCAAG+AGG | 0.613572 | 7.3:-65343030 | MS.gene62748:CDS |
| CATAGGTGATGGGTTTCAGA+AGG | 0.615780 | 7.3:-65343084 | MS.gene62748:CDS |
| CATAGATGAGGAATTTCAAG+AGG | 0.630357 | 7.3:-65343138 | MS.gene62748:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATGTAAAAATTTAAAGTTA+CGG | - | chr7.3:65343086-65343105 | MS.gene62748:CDS | 10.0% |
| !! | AAATTGTGTTTAACTAATAT+AGG | - | chr7.3:65343110-65343129 | MS.gene62748:CDS | 15.0% |
| !! | AAGTTGAAGAAACTTAAAAA+TGG | - | chr7.3:65343364-65343383 | MS.gene62748:intron | 20.0% |
| !! | AGTTGAAGAAACTTAAAAAT+GGG | - | chr7.3:65343365-65343384 | MS.gene62748:intron | 20.0% |
| !! | TATATATGATGAATTTCAAG+AGG | - | chr7.3:65343731-65343750 | MS.gene62748:intron | 20.0% |
| !!! | AATTATTTTCATATGCAACA+AGG | - | chr7.3:65343600-65343619 | MS.gene62748:CDS | 20.0% |
| !!! | TTTTATTTTCATATGCAACA+AGG | - | chr7.3:65343558-65343577 | MS.gene62748:CDS | 20.0% |
| ! | CTTTGAATCAACAATATCTA+CGG | - | chr7.3:65343522-65343541 | MS.gene62748:intron | 25.0% |
| ! | TGTAATGATAGAATTAGGAA+GGG | - | chr7.3:65343390-65343409 | MS.gene62748:intron | 25.0% |
| ! | TTGTAATGATAGAATTAGGA+AGG | - | chr7.3:65343389-65343408 | MS.gene62748:intron | 25.0% |
| ! | TTGTGTTTAACTAATATAGG+CGG | - | chr7.3:65343113-65343132 | MS.gene62748:CDS | 25.0% |
| ACTTCAAGAAATGGTTGAAA+AGG | - | chr7.3:65343189-65343208 | MS.gene62748:CDS | 30.0% | |
| ATGAAATTCAAGAGGATGAT+CGG | - | chr7.3:65343766-65343785 | MS.gene62748:CDS | 30.0% | |
| CAAAACAAATGTTCTACTTG+CGG | - | chr7.3:65343420-65343439 | MS.gene62748:intron | 30.0% | |
| TCTAGATGATGAAATTCAAG+AGG | - | chr7.3:65343758-65343777 | MS.gene62748:CDS | 30.0% | |
| TGAAAAAGATGACATAGATG+AGG | - | chr7.3:65343638-65343657 | MS.gene62748:intron | 30.0% | |
| TTCAACCATTTCTTGAAGTT+GGG | + | chr7.3:65343188-65343207 | None:intergenic | 30.0% | |
| TTTCAACCATTTCTTGAAGT+TGG | + | chr7.3:65343189-65343208 | None:intergenic | 30.0% | |
| !! | CGTAGATATTGTTGATTCAA+AGG | + | chr7.3:65343524-65343543 | None:intergenic | 30.0% |
| !!! | ATGTTTTTTGAAACTCGACA+AGG | - | chr7.3:65343019-65343038 | MS.gene62748:CDS | 30.0% |
| !!! | ATTTGGTTTTTATTTGTGCG+AGG | - | chr7.3:65343471-65343490 | MS.gene62748:intron | 30.0% |
| !!! | GAAGCTTTTAAAGCTGTTAT+GGG | - | chr7.3:65343226-65343245 | MS.gene62748:intron | 30.0% |
| !!! | TTTTAAAGCTGTTATGGGAA+AGG | - | chr7.3:65343231-65343250 | MS.gene62748:intron | 30.0% |
| AAGCAATTGGATGTAGAAAC+TGG | - | chr7.3:65343052-65343071 | MS.gene62748:CDS | 35.0% | |
| CATAGATGAGGAATTTCAAG+AGG | - | chr7.3:65343650-65343669 | MS.gene62748:intron | 35.0% | |
| CGCATATCATGAATTTCTTG+AGG | - | chr7.3:65343677-65343696 | MS.gene62748:intron | 35.0% | |
| GAATTTCTTGAGGACAACAT+AGG | - | chr7.3:65343687-65343706 | MS.gene62748:intron | 35.0% | |
| GGGCTTGTAATGATAGAATT+AGG | - | chr7.3:65343385-65343404 | MS.gene62748:intron | 35.0% | |
| TCAAGAAATGGTTGAAAAGG+AGG | - | chr7.3:65343192-65343211 | MS.gene62748:CDS | 35.0% | |
| !! | GCGTGTTTTACTTGTTGATT+TGG | - | chr7.3:65343454-65343473 | MS.gene62748:intron | 35.0% |
| !!! | CGAAGCTTTTAAAGCTGTTA+TGG | - | chr7.3:65343225-65343244 | MS.gene62748:intron | 35.0% |
| AACACAATCAAAATCCGACC+TGG | + | chr7.3:65343265-65343284 | None:intergenic | 40.0% | |
| AGGAAGTAGAGGAAAGCAAT+TGG | - | chr7.3:65343039-65343058 | MS.gene62748:CDS | 40.0% | |
| CTAGTCCCAACTTCAAGAAA+TGG | - | chr7.3:65343180-65343199 | MS.gene62748:CDS | 40.0% | |
| TTGAGGACAACATAGGTGAT+GGG | - | chr7.3:65343694-65343713 | MS.gene62748:intron | 40.0% | |
| CTTGAGGACAACATAGGTGA+TGG | - | chr7.3:65343693-65343712 | MS.gene62748:intron | 45.0% | |
| GAAACTCGACAAGGAAGTAG+AGG | - | chr7.3:65343028-65343047 | MS.gene62748:CDS | 45.0% | |
| !! | CATAGGTGATGGGTTTCAGA+AGG | - | chr7.3:65343704-65343723 | MS.gene62748:intron | 45.0% |
| ! | ATGGGAAAGGAGTGTGTACC+AGG | - | chr7.3:65343244-65343263 | MS.gene62748:intron | 50.0% |
| ! | GAAAGGAGTGTGTACCAGGT+CGG | - | chr7.3:65343248-65343267 | MS.gene62748:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 65343019 | 65343791 | 65343019 | ID=MS.gene62748 |
| chr7.3 | mRNA | 65343019 | 65343791 | 65343019 | ID=MS.gene62748.t1;Parent=MS.gene62748 |
| chr7.3 | exon | 65343732 | 65343791 | 65343732 | ID=MS.gene62748.t1.exon1;Parent=MS.gene62748.t1 |
| chr7.3 | CDS | 65343732 | 65343791 | 65343732 | ID=cds.MS.gene62748.t1;Parent=MS.gene62748.t1 |
| chr7.3 | exon | 65343545 | 65343626 | 65343545 | ID=MS.gene62748.t1.exon2;Parent=MS.gene62748.t1 |
| chr7.3 | CDS | 65343545 | 65343626 | 65343545 | ID=cds.MS.gene62748.t1;Parent=MS.gene62748.t1 |
| chr7.3 | exon | 65343019 | 65343230 | 65343019 | ID=MS.gene62748.t1.exon3;Parent=MS.gene62748.t1 |
| chr7.3 | CDS | 65343019 | 65343230 | 65343019 | ID=cds.MS.gene62748.t1;Parent=MS.gene62748.t1 |
| Gene Sequence |
| Protein sequence |
